LOCUS       NZ_CP014859            10414 bp    DNA     circular CON 20-NOV-2024
DEFINITION  Actinoalloteichus hymeniacidonis strain HPA177 chromosome, complete
            genome.
ACCESSION   NZ_CP014859
VERSION     NZ_CP014859.1
KEYWORDS    RefSeq.
SOURCE      Actinoalloteichus hymeniacidonis
  ORGANISM  Actinoalloteichus hymeniacidonis
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Pseudonocardiales; Pseudonocardiaceae; Actinoalloteichus.
REFERENCE   1  (bases 1 to 6306386)
  AUTHORS   Schaffert,L., Albersmeier,A., Winkler,A., Kalinowski,J.,
            Zotchev,S.B. and Ruckert,C.
  TITLE     Complete genome sequence of the actinomycete Actinoalloteichus
            hymeniacidonis type strain HPA 177T isolated from a marine sponge
  JOURNAL   Stand Genomic Sci 11, 91 (2016)
   PUBMED   28031775
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6306386)
  AUTHORS   Schaffert,L., Albersmeier,A., Winkler,A., Kalinowski,J., Zotchev,S.
            and Ruckert,C.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAR-2016) CeBiTec, Bielefeld University, Sequenz 1,
            Bielefeld, NRW 33615, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Newbler v. 2.8
            Genome Coverage                   :: 159x
            Sequencing Technology             :: Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_001747425.1-RS_2024_11_18
            Annotation Date                   :: 11/18/2024 01:17:00
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,434
            CDSs (total)                      :: 5,374
            Genes (coding)                    :: 5,337
            CDSs (with protein)               :: 5,337
            Genes (RNA)                       :: 60
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 3 of 37
            Pseudo Genes (incomplete)         :: 34 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            CRISPR Arrays                     :: 13
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 14:46:53
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 1526873
            Orig. end                         :: 1537287
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP014859.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10414
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="ectoine"
                     /region_number="6"
                     /rules="ectoine_synt"
                     /tool="antismash"
     cand_cluster    1..10414
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="ectoine_synt"
                     /kind="single"
                     /product="ectoine"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10414
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5414](+)"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5414
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
     misc_feature    complement(370..384)
                     /note="TFBS match to MexT, Global virulence regulator,
                     confidence: weak, score: 17.3"
                     /tool="antismash"
     gene            complement(657..1622)
                     /locus_tag="TL08_RS07075"
                     /old_locus_tag="TL08_07175"
     CDS             complement(657..1622)
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF017085.5"
                     /locus_tag="TL08_RS07075"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="TL08_07175"
                     /product="CHAD domain-containing protein"
                     /protein_id="WP_069847562.1"
                     /transl_table=11
                     /translation="MTTETPQVSPSTTRIDKKSSAGEVVLAYLHEQAAALRSWEDAFRR
                     GEPDAVHRMRIAVRRMRSALQAFGEVVDREQTRGLTSELRWLAEVLGESRDVEVVYALL
                     KERTERTPPELLLGAVDQHITRDLARRQADSDQAVSEALDGPRHRELTESIAGLLAQPP
                     LTPLAAERARTVLPASVARTYRKLDRAARAVGAPPWDRASIALDDADTDQDQRLHETRK
                     LAKRMRYAVEVVRPITGKKVRRLGRAVRRLQRTLGTRQDIVLTRQALRRLGAQATATGG
                     NGFTFGLWYGHDHGRAAALEQRFAEDWRRVTRSRSARWTG"
     misc_feature    complement(1428..1430)
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     misc_feature    1641..1656
                     /note="TFBS match to RutR, Regulator of the pyrimidine and
                     purine metabolism, confidence: weak, score: 20.8"
                     /tool="antismash"
     gene            1932..2192
                     /locus_tag="TL08_RS29750"
                     /old_locus_tag="TL08_07180"
     CDS             1932..2192
                     /GO_process="GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009945846.1"
                     /locus_tag="TL08_RS29750"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="TL08_07180"
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="WP_069847564.1"
                     /transl_table=11
                     /translation="MFGLGATELLIIAGVLVLLFGATKLPQMARSLGQSARILKAETKG
                     FRGDKGETPAVEAAGSEAPGVQAAQVQQPVQTAPSAPVDKS"
     gene            2279..3223
                     /gene="tatC"
                     /locus_tag="TL08_RS07085"
                     /old_locus_tag="TL08_07185"
     CDS             2279..3223
                     /GO_component="GO:0005886 - plasma membrane [Evidence IEA]"
                     /GO_process="GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /gene="tatC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016698017.1"
                     /locus_tag="TL08_RS07085"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="TL08_07185"
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="WP_236750530.1"
                     /transl_table=11
                     /translation="MIRWIRSLRWLKVLRFGKGGRRGNPDGTMPLIEHLYELRYRLALA
                     FGALMIGAVIGFIWFDYGPGPIPTLSSLITGPYCSLPTNMRFQPNDGACQLMQTTAFEV
                     VSLRIQLALASGAVLSSPMWLYQIWAFITPGLYSKERKFAGIFVGCASVLFIFGAVLAY
                     LVAPEGLYFMASFGAGNFFTALTGGAYVGFILLLLLIFGISFELPLLVVMLNRAGILPY
                     AKLKQWWRGIVFGLFVFAAFATPGQDPLSMVVLATALVLLFGAASQFARIHDKRKAKKL
                     AAAGLAELDPDQASEIDTKPSELPKPDRYDDAT"
     misc_feature    3471..3484
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 15.68"
                     /tool="antismash"
     gene            3744..4937
                     /locus_tag="TL08_RS07090"
                     /old_locus_tag="TL08_07190"
     CDS             3744..4937
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /GO_process="GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009947881.1"
                     /locus_tag="TL08_RS07090"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="TL08_07190"
                     /product="Glu/Leu/Phe/Val dehydrogenase dimerization
                     domain-containing protein"
                     /protein_id="WP_069853370.1"
                     /transl_table=11
                     /translation="MALIDDDPLVRVTWTDRETGVRGYLVVHTLVSGLATGGTRMRAGC
                     TLSEVEDLARGMAIKAAVFDLPVGGAKGGIDCDPKDPRARGVLRRFVQAMRPWLDDHWV
                     TAEDLGVPQHVIDEIFGELGLDQSYHAAIHRSVNPALTLRRVRAGLNSPVPGGYLLGDV
                     VGGYGVAQSCLGVAAARGWRTSETTVAVQGVGTMGGGAAWYLHEAGLRIVMVADAAGAL
                     YDPAGLDIPALLAARDRFGEIDRSRVPERVARLPKESVLSVEVDLLVPAAVSYAITEQN
                     ADAVAAGVVVEAANVATTPAAERRLAERGVPVMPDFVVNAGAAAWAWWLLLGRVDADPA
                     LSFSVLRQEMLARIAPLVSAWDHGAVPPRTTALAAADLQVERHRAAEADGRRLVTIP"
     gene            5001..5414
                     /locus_tag="TL08_RS07095"
                     /old_locus_tag="TL08_07195"
     CDS             5001..5414
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     ectoine: ectoine_synt"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009947387.1"
                     /locus_tag="TL08_RS07095"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="TL08_07195"
                     /product="ectoine synthase"
                     /protein_id="WP_069853371.1"
                     /sec_met_domain="ectoine_synt (E-value: 1.2e-51, bitscore:
                     172.2, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIVRSVVDIEDGDRDVKTPNWRSKRIVLAKEGVGFSVHETTLYAG
                     TVNDFWYANHVEAVFVVEGEGELLNKETGEVHALSPGTLYLLDGNERHQLRPTTLMRTV
                     CVFNPPVTGAEVHDENGVYPLIVEPAPDPAPSA"
     tRNA            complement(5521..5593)
                     /anticodon=(pos:complement(1532431..1532433),aa:Arg,
                     seq:ccg)
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /locus_tag="TL08_RS07100"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /old_locus_tag="TL08_t07200"
                     /product="tRNA-Arg"
     gene            complement(5521..5593)
                     /locus_tag="TL08_RS07100"
                     /old_locus_tag="TL08_t07200"
     gene            5826..6755
                     /locus_tag="TL08_RS07105"
                     /old_locus_tag="TL08_07205"
     CDS             5826..6755
                     /GO_function="GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009948448.1"
                     /locus_tag="TL08_RS07105"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="TL08_07205"
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="WP_069847566.1"
                     /transl_table=11
                     /translation="MGHGHGHGVSAVSASQKQLKRLGWAFGVLLVFLVVEVIVGLATSS
                     LALLSDAGHMLTDVLGIGMALAAITAARTSAKKARGGGSSRTFGLYRLEVLAALANAVL
                     LLGVAGFILVEAVGRLADPPEIAAGPVMIAAAAGLAANLVAFALLRPAASESINMRGAY
                     LEVLADTIASIGVLLGTATSLFFGWTLADPIVAVAIAVFVVPRTLRLAGQALRILVQQA
                     PADLDIGELTDRLRAVPGVAEVHDLHVWTLTSGMEVLAAHLTVDAETRTDDVLASAQRM
                     LAERYEIEHATLQVESTTSAVRCQELTW"
     misc_feature    6267..6269
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            complement(6823..7269)
                     /locus_tag="TL08_RS07110"
                     /old_locus_tag="TL08_07210"
     CDS             complement(6823..7269)
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013224776.1"
                     /locus_tag="TL08_RS07110"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="TL08_07210"
                     /product="nitroreductase/quinone reductase family protein"
                     /protein_id="WP_069847568.1"
                     /transl_table=11
                     /translation="MKPPPPSSPYWKFHRQLARFNKVVFRRTKGRLGGTFAGSPVLLLD
                     HVGRRSGQDRTSPLIYLDDSPNLVIVASKGGVDAHPSWFHNVMAMDSTEVELPGGIRRR
                     VRPRVAEGAERESLWARLVDLYKPYEAYATHTERRIPVVVLEPA"
     misc_feature    7336..7349
                     /note="TFBS match to LexA_variant_2, Repressor of DNA
                     damage response, confidence: weak, score: 18.09"
                     /tool="antismash"
     misc_feature    complement(7336..7349)
                     /note="TFBS match to PsrA, Oleic acid responsive regulator,
                     confidence: weak, score: 18.52"
                     /tool="antismash"
     gene            7375..8022
                     /locus_tag="TL08_RS07115"
                     /old_locus_tag="TL08_07215"
     CDS             7375..8022
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1215: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013224777.1"
                     /locus_tag="TL08_RS07115"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="TL08_07215"
                     /product="TetR family transcriptional regulator"
                     /protein_id="WP_069847571.1"
                     /transl_table=11
                     /translation="MTEPGLRERKKQRTRDALTEAAYVLFDRKGFEATTIDEIADAVEV
                     SPRTFLRYFNSKEDVALTSANQQLTAMIENLAARPSDESVLTALRRSVVEVLEACETGR
                     DGFDRDRYQRTRTLLARTPTLLAASLEQSAIRLDEVATLIGQRMGVDHTVDRRPYLVAA
                     VALNAVQTGLNAWRAHHPAALASETVGELFDLLGEGIDYPAAVPPVSDDEPG"
     gene            complement(8091..8885)
                     /locus_tag="TL08_RS07120"
                     /old_locus_tag="TL08_07220"
     CDS             complement(8091..8885)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009948449.1"
                     /locus_tag="TL08_RS07120"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="TL08_07220"
                     /product="DUF305 domain-containing protein"
                     /protein_id="WP_236750532.1"
                     /transl_table=11
                     /translation="MTDGEIEDASARRGSAADAAEPVADDRADRESDDVEPERASSSGG
                     PNWVRILVASAAVLTLLLLGAAAGMLIGLPGAANRAAPPADSVDVGFAQDMSVHHRQAT
                     QMATLYRANGTQDDILTLAFDIDTNQRAQIGWMQGWLNLWDRPLNPPGEYMAWMSEGGG
                     HGHGGAEPGASPEEAMPGMASQEELERLRSLRGTELDIFFLQLMLRHHEGGTEMAAYAA
                     ERAAIPQVSNLASNMLNHQTAESRKMTGMLSDLGAEPLPFEG"
     gene            complement(8901..9863)
                     /locus_tag="TL08_RS07125"
                     /old_locus_tag="TL08_07225"
     CDS             complement(8901..9863)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016699862.1"
                     /locus_tag="TL08_RS07125"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="TL08_07225"
                     /product="DUF3105 domain-containing protein"
                     /protein_id="WP_084642672.1"
                     /transl_table=11
                     /translation="MASGKSAKALRNARAAMVTEKQRPWGTVAAVAAVVVFAAGVFGYV
                     YVRYDATEDIRAFTPTAENQDPSTAIEGIVIEEFEAQQHVVAPTRVAYESSPPFGGPHD
                     GVWAGCNGEIYDEAVRTENLIHSLEHGAVWIAYNPDQVEGEQLGLLEDRVRNQAAMVMS
                     PYPGLDSPISLQAWGHQLKLDDASDERIDQFIMALQLNAYTYPEVGATCEANPQAFDVD
                     NPPPFDPSEPGPDAMPMNGNPEDFGGFTGEGDQDMSQIDPNAPVDPDAPADGGEGDADT
                     EGGDGAEGGEDAENPEGSEDAPADGEDGSEPADGTETND"
ORIGIN
        1 cgccccggtg accagcatca acatcgagaa gaacttcgcc gcggctacac cggtgtagag
       61 atcgcggatg atggctcttg cgatgacgag tccggccgca ccggccatgc cctgaatgaa
      121 gcgcaggccc accagcgcgg cggtcgacgg cgcgaccgcg caaagcagcg atgcgaccag
      181 gtacacggcc acgccgacca gcagcggtcg tctgcggccc agggcatcgc tgagtggtcc
      241 cgccacgagc tggccgaagg ccaacccgat cagacacgcg gtgagggtca gttggatctg
      301 ggccgccgtc gagctgaact cgacactgag atcggggaag gcgggcaggt acatgtcgat
      361 ggacagggga ccgacggcgg tgatggtgcc caggatgagg gccagcttcc accgggatgg
      421 tgtggatcga gcggttcggg tcgtgtcggt gagttgaccg gtcaagctca acgaattctc
      481 cagacgggta caggaggtct cgggtgaagc gcataccccc ggccgtccaa cacgacgctg
      541 gttcgagtcc atctcgaaag tgacgtatct aacccgtttg gcggactatc tttccgatgg
      601 ttgggaactc acggcgactt ttgctcgttt cgtcgcccga tggcgagagc gtggcttcac
      661 cccgtccagc gcgccgatcg ggaccgggta acccgtcgcc agtcctcggc gaagcgctgc
      721 tccagcgctg cggcacggcc gtggtcgtgg ccgtaccaga ggccgaaggt gaagccgttc
      781 ccaccggtcg ccgtcgcctg ggcgcccagc cgacgcaggg cctgtctggt cagcacgatg
      841 tcctgccggg ttcccagggt gcgctgcaag cgtcgcaccg ctcggccgag acgacgcacc
      901 ttcttaccgg tgatgggacg gacgacctcg accgcgtagc gcatccgctt ggccagcttc
      961 ctggtctcgt gcaggcgctg gtcctggtcg gtatcggcat cgtcgagcgc gatcgaggcc
     1021 cggtcccacg gcggagcgcc cacggcccgt gcggcccggt ccagcttccg ataggtacgg
     1081 gccaccgagg cgggcagcac cgtgcgggcc cgctcggccg cgagcggggt cagcggcggc
     1141 tgggcgagca atcccgcgat cgactcggtg agttcgcggt gccgggggcc gtccagcgcc
     1201 tcggacaccg cctgatccga gtcggcctgc ctgcgggcca ggtctcgggt gatgtgctgg
     1261 tcgacggcgc cgagcagcag ttcgggcggg gtgcgctcgg tgcgctcctt gagcagcgca
     1321 tagacgacct cgacatccct ggactccccg agcacctccg ccagccatct cagctcggac
     1381 gtcaaccctc gggtctgctc tcgatccacg acctcgccga aggcctgtaa ggcactgcgc
     1441 atccgccgca cggcgatccg catccgatgg accgcatccg gctcgccccg gcggaaggcg
     1501 tcctcccacg atcggagggc ggcggcctgc tcgtgcaggt aggcgaggac gacctctccc
     1561 gcactggact tcttgtcgat ccgggtcgtc gacgggctga cctgcggtgt ctccgtagtc
     1621 atctgcgctc ctgccctgct tggacggact ggtctgctgc cctgcaggta cccgatgggc
     1681 acgccgatga cacggtcgag atcaccggat cagacgacta tcgtcgaacc ctcctacctg
     1741 ggaaaatgcg gtgacttgcc ggtatgtgct cgacgtcgtc gtgcgccggg tcgatcgaga
     1801 tggtctgcgc gagcacgcag atcggcagtg gctccgaagg aaagtcgtgg acaacgttgg
     1861 gtcaacgccc tcgctctcga ccgggtcgaa tgcgtacgat cggctcgagc cactgattgg
     1921 aggccccccg aatgttcggt ctcggtgcta ctgagctgct gatcatcgca ggtgttctcg
     1981 tgctgctgtt cggcgcgacc aagctgccgc agatggcgcg gtcgctcggt cagtccgcgc
     2041 gcatcctcaa ggctgagacg aagggattcc gcggcgacaa gggtgagacc ccagccgtgg
     2101 aggccgccgg ttccgaggcg ccgggagtcc aggccgccca ggtccagcag ccagtccaga
     2161 ccgccccctc ggcgccggtc gacaagagct gagtcgtctc gcccggcctg acggccggtc
     2221 gcgggagatc ggctcttcag ccgatcgtta ggaagacaga gaaggcaggc ccctggtcgt
     2281 gatccgctgg attcggtcgc tgcgctggtt gaaggtgctt cgtttcggca agggcggcag
     2341 gcgggggaat cccgacggca cgatgccgct catcgagcac ctctacgaac tgcgctatcg
     2401 attggcgttg gccttcggcg ccctgatgat cggcgcggtc atcggtttca tctggttcga
     2461 ctacgggccg ggaccgattc cgacgctgtc ctcgttgatc acggggccgt actgctcgct
     2521 gccgaccaac atgcggttcc agcccaacga cggcgcctgc cagctcatgc agaccacggc
     2581 gttcgaggtg gtctcgctgc gaatccaact cgccctggcc tccggtgcgg tgctgtcctc
     2641 accgatgtgg ctctaccaga tctgggcgtt catcacgcca ggcctgtact ccaaggaacg
     2701 caagttcgcg ggcatcttcg tcggctgcgc gagcgtgctg ttcatcttcg gtgcggtgct
     2761 cgcctacctg gtcgcccccg agggcctcta cttcatggcc agcttcggcg cggggaactt
     2821 cttcacggcg ctgaccggtg gtgcctatgt cggcttcatc ctgctgttgc ttctgatctt
     2881 cggcatcagc ttcgaactgc ccttgctggt ggtcatgctc aaccgcgcgg gcatcctgcc
     2941 ctacgcgaag ttgaagcagt ggtggcgcgg catcgtcttc gggctgttcg tcttcgccgc
     3001 gttcgctacc cccggtcagg acccactgtc gatggtggtg ctcgcgaccg cgctggtcct
     3061 gctgttcggt gcggcaagtc agttcgcccg gatacacgac aagcgcaagg cgaagaagct
     3121 ggccgccgcg ggcttggccg agttggatcc cgatcaggcc tcggagatcg acaccaagcc
     3181 cagcgagttg cccaagcccg accgttacga cgacgccacc tgatcgatcc ccgtcggcgt
     3241 ccgacgtcga caccccgcat cgccgcgccc gtggcccgaa ccaggggccg ccggcgtttc
     3301 ggcgttcgcg ccctgctgtg gcgccgcgcg ggctagccgc ctgcgccgcg gacggtctgc
     3361 tcgattcgtc cgagtgggtg gatctcatcg aaccccgtta tcgaaatgag cccgtgacgt
     3421 gctgcgcagg gtttgtgggt ttcacctgca cgattttcgg ggatcgtcag attgttgaca
     3481 ataaatcggc tgcggagcta gctttcggtg acgccccgga ggcagagcgt ggaggtgcca
     3541 agccatggct atcactagtg gtcaatgggc ctggctctgc ctactgcggg tcttctcatc
     3601 tcggctgcag tagcccgtct cgggcgcagc ggcgtgcggc ggcacagctt ggtgccgccg
     3661 gtgcacgcac ccctcatcag cttgggtcgg gtcgcttggc gggcgctcgg cggctgttcg
     3721 gcataggaga ggaggttcgg ctggtggctc tcatcgacga cgacccgctg gtgcgggtga
     3781 catggactga ccgggagacc ggggtacgcg gctatctggt ggtgcacacc ctggtatccg
     3841 gtctagccac cgggggcacc aggatgcgcg cgggctgcac cctgtcggag gtcgaggacc
     3901 tggccagggg gatggcgatc aaggccgccg tgttcgatct gccggtgggc ggtgccaagg
     3961 gcggtatcga ctgcgacccg aaggacccgc gagcccgcgg ggtactgcga cgtttcgtac
     4021 aggcgatgcg gccctggctg gacgatcatt gggtcacggc cgaggacctc ggcgttccgc
     4081 agcacgtgat cgacgagatc ttcggcgaac tggggctcga ccagtcttac cacgcggcca
     4141 tccaccggtc ggtcaacccc gcgctgaccc tgcgacgggt tcgggccgga ttgaactccc
     4201 cggtcccggg cggctacctg ctcggcgacg tggtgggcgg ttacggcgtc gcgcagagct
     4261 gcctcggggt ggccgccgcc cgtggctggc ggacctcgga gacgacggtg gccgtgcagg
     4321 gcgtcggcac catgggcggc ggtgcggcct ggtatttgca tgaggcggga ctgcggatcg
     4381 tgatggtcgc cgatgcggcc ggggccctct acgacccggc cggactggac atacccgcgt
     4441 tgttggcggc gcgggaccgc ttcggtgaga tcgaccgaag ccgggtgccg gagcgggtgg
     4501 ctcggctgcc caaggaatcg gtgctcagcg tggaggtgga tctcctcgtc ccggcggcgg
     4561 tctcctacgc gatcaccgaa cagaacgccg acgcggtggc cgcaggcgtg gtcgtcgagg
     4621 cagccaacgt cgcgaccacc ccggcggcgg agcgcaggct cgccgagcgc ggagtccccg
     4681 tcatgccgga cttcgtggtc aacgcgggcg cagcggcctg ggcctggtgg ctgctgctcg
     4741 gcagggtgga cgccgatccc gcgttgtcgt tctcggtgtt gcgtcaggag atgctggcca
     4801 ggatcgcgcc gctggtctcg gcgtgggacc acggcgcggt gccgcctagg accacggcgc
     4861 tggcggcggc cgatctacag gtggaacggc atcgagcggc cgaggcggac ggccgtcgat
     4921 tggtgaccat tccatgacgg ggttgacggc cccatgacgg agttgacggc aatgttcggc
     4981 gcaagcgagt ggagacggcg atgatcgtgc gttccgtggt cgatatcgag gacggcgacc
     5041 gggatgtgaa gacccccaac tggcgcagca agcgcatcgt gctggccaag gagggcgtcg
     5101 gtttctcggt gcacgagacg accctctacg cggggacggt gaacgacttc tggtacgcca
     5161 atcacgtcga agccgtcttc gtcgtggagg gcgagggcga gctgctgaac aaggagaccg
     5221 gcgaggtgca cgccctgtcg cccggcaccc tgtatctcct ggacggcaac gaacgtcatc
     5281 agctccgccc gacgacgctg atgcgtaccg tctgcgtgtt caacccgccg gtgaccggcg
     5341 cggaggtgca cgacgagaac ggtgtgtacc cgttgatcgt ggagcctgcg ccggatccgg
     5401 caccatcggc ctgagtcggg gcaaccgaag acatcggctg ccttagaggg atcgagtccg
     5461 agaacgaaaa tgcccatgag cgcggcgtgg cggctcatgg gcattttcgt gtttccggtg
     5521 tgccgtcggc aggaatcgaa cctgcgacac ctgcctccgg agggcagtgc tctatcccct
     5581 gagctacgac ggcatgtcgt ttgtggcgac gggagtaatc ctagcgcacg cccagcagtg
     5641 ctttgctcgg gcctgccctg ttgggttgcg caagacgatc tcgctggtca gggccgtgtc
     5701 ggttcggtgg cggcgatggg ggtccggttc tggtgccgcg cacccgatgg cgccgtccgg
     5761 gtggcgtgct cggaatgaga gcgacatcga ttgcattcat gcgcatgtca ctatatgttc
     5821 tggccatggg tcacggtcat gggcacggtg tgtccgcagt cagcgcatcg cagaagcagc
     5881 tgaaacgact cggctgggca ttcggcgtcc tgctcgtgtt cctagtggtc gaggtgatcg
     5941 tcggcctggc cacgtcctcg ttggcgctgt tgtccgatgc gggacacatg ctcaccgacg
     6001 tgctgggcat cggcatggcg ctggccgcca tcaccgcggc tcgtacctcg gccaagaagg
     6061 cacgcggtgg aggttcttcg cgcaccttcg ggctctatcg gctggaggtg ctcgcggcgc
     6121 tggccaacgc ggtgctgctg ctcggcgtcg caggcttcat cctggtggag gcggtcgggc
     6181 gactcgccga tccgccggag atcgccgcgg gtccggtgat gatcgcggcg gccgcaggcc
     6241 tggcggccaa cctggtggcc ttcgcgttat tgcgtccggc ggcctcggag agcatcaaca
     6301 tgcgaggtgc ctacctggag gttctcgccg acaccatcgc ctccatcggg gtgctgctgg
     6361 gcacggcgac gagcctgttc ttcggctgga cgctggcgga cccgatcgtc gcggtggcca
     6421 tcgccgtctt cgtggtgccg cgcaccctgc gactcgcggg ccaagcgctg cgcatcctcg
     6481 tccagcaggc accggctgat ctggatatcg gcgagttgac cgaccgcctt cgcgccgtgc
     6541 ccggcgtcgc ggaggtgcat gacctgcacg tctggacgct cacctccggg atggaggtgc
     6601 tcgccgcgca cctcaccgtc gacgccgaga cccgcacgga cgacgtgctg gcctcggcgc
     6661 aacggatgct ggcggagcga tacgagatcg aacacgccac cctgcaggtc gagtcgacga
     6721 cgagcgcggt ccgctgtcag gagctgacct ggtgatcggg cggctgcgga ctcgccgtcg
     6781 gtccgtcgat ggattggatg acgcagctgg cgtgagttcg ggtcaggccg gttcgagcac
     6841 gaccaccggg atccggcgct cggtgtgggt ggcgtaggcc tcgtacggct tgtacagatc
     6901 gacgagccgt gcccagagtg attcgcgctc ggcgccctcg gcgaccctcg ggcgcaccct
     6961 cctgcggatc ccgcccggca gctcgacctc ggtgctgtcc atcgccatca cgttgtggaa
     7021 ccaggacggg tgcgcgtcca ccccgccctt ggacgcgacg atgaccaggt tcgggctgtc
     7081 gtcgagatag atgagcgggc tcgtcctgtc ctgcccgctc cggcgaccga cgtggtcgag
     7141 caggagcacc ggcgacccgg cgaacgtccc gccgaggcgg cccttggttc gacggaagac
     7201 gaccttgttg aaccgggcga gctggcggtg gaacttccag tacggcgagg acggtggcgg
     7261 tggcttcatt gcactattct gtcggcaagt gacaaatgtc gtcaatcggc tttagtttta
     7321 cagtggcata agtcacgaac aggcgttttg gctgacgcga cctatgctgt tgcggtgacg
     7381 gaaccagggc tgcgcgaacg gaagaagcag cggacacgag acgcgttgac cgaagccgcg
     7441 tacgtcctgt tcgaccgcaa gggcttcgag gcgacgacca tcgacgagat cgccgacgcc
     7501 gtcgaggtct cgccgaggac cttcctgcgc tacttcaact cgaaagagga tgtggcgctg
     7561 acctcggcga accagcagct cacggcgatg atcgagaatc tcgccgcgcg cccgtcggac
     7621 gaatcggttc tcacggcgtt gcgccgctcc gtcgtcgagg tcctggaagc ctgcgagaca
     7681 ggcagagacg gcttcgaccg agatcggtat caacgaacca gaacgctgtt ggcgaggact
     7741 ccgaccctgc tggccgcatc actggaacag agtgccatcc ggctcgacga ggtggccacg
     7801 ttgatcgggc agcggatggg cgtcgaccat accgtcgatc gcaggccgta tctcgtggcc
     7861 gccgtcgcgc tgaacgcggt ccagaccgga ctcaatgcct ggcgggccca ccatccggcg
     7921 gcactggcct ccgagacggt aggcgagttg ttcgatctgc tcggcgaggg catcgactac
     7981 ccggctgcgg tcccgccggt cagtgacgac gaaccggggt aggccatggt ggcgtacccc
     8041 ggtcggtgtc gttcagcagc ctgctcgccg tacgagccgg tggcgggccg tcaaccctcg
     8101 aagggcaacg gctcggcgcc cagatcggac agcatgccgg tcatctttcg ggactccgca
     8161 gtctgatgat tgagcatgtt ggacgccagg ttgctcacct ggggaatggc agcccgctcg
     8221 gccgcgtacg cggccatctc ggtgccgccc tcgtgatgcc gcagcatcaa ctgcaggaag
     8281 aagatgtcga gctcggtgcc gcgcagcgaa cgcaaccgct ccagctcctc ctgcgacgcc
     8341 atacccggca tggcctcctc gggactggcc cccggctcgg cgccgccgtg cccatgaccg
     8401 ccgccctcgc tcatccaggc catgtactcg ccagggggat tcagcggccg gtcccacagg
     8461 ttcaaccagc cctgcatcca accgatctgc gctcgctggt tggtgtcgat gtcgaaggcg
     8521 agcgtgagga tgtcgtcctg cgttccattg gcccggtaga gcgtcgccat ctgcgtcgcc
     8581 tgccggtggt gcaccgacat gtcctgagcg aagccgacgt ctaccgagtc cgccgggggt
     8641 gcggcccgat tcgccgcccc gggcaatccg atgagcatcc cggccgccgc accgagtagc
     8701 agcagggtga gcacggctgc cgaggcgacc aggatgcgca cccagttggg cccgccggag
     8761 gacgaggccc gttcgggttc gacgtcatcg ctctcgcggt cggcccggtc gtcggcgacg
     8821 ggctcggccg cgtctgcggc cgagccacgg cgtgcggagg cgtcctcgat ctcgccatcg
     8881 gtcacggtgt aagccggtcc ctagtcgttg gtctcggtgc cgtcggcagg ctcggacccg
     8941 tcctcgccgt ccgcaggagc gtcctccgaa ccctcggggt tctcggcgtc ctcgccaccc
     9001 tcggcgccgt caccgccttc ggtgtccgca tcgccctcgc cgccatcggc gggtgcatcg
     9061 gggtcgaccg gggcgttcgg gtcgatctgg ctcatgtcct ggtcgccctc gccggtgaag
     9121 ccaccgaagt cctcgggatt gccgttcatc ggcatcgcgt ccggaccggg ctcgctcggg
     9181 tcgaacggcg gcgggttgtc gacgtcgaac gcctgcgggt tggcttcgca ggtcgcgccg
     9241 acctcggggt aggtgtaggc gttgagctgc aaggccatga tgaactggtc gatgcgctcg
     9301 tccgaggcat cgtccagctt gagctggtgg ccccaggcct gcagcgagat cgggctgtcc
     9361 aacccggggt acggcgacat caccatcgcg gcctggttcc ggacccggtc ctcgagcagt
     9421 ccgagctgct cgccctcgac ctggtcgggg ttgtaggcga tccacaccgc gccgtgttcg
     9481 agcgagtgga tcaggttctc ggtgcgcacg gcctcgtcgt agatctcgcc gttgcaaccc
     9541 gcccacacac cgtcatgcgg accgccgaac ggcggcgacg actcgtaggc gacgcgcgtc
     9601 ggcgcgacga cgtgctgctg cgcctcgaat tcctcgatga cgatgccctc gatagcggtc
     9661 gaggggtcct ggttctccgc cgtcggagtg aaggccctga tgtcttccgt cgcgtcgtag
     9721 cgcacgtaca cataaccgaa gacacctgcc gcgaagacga cgacggcggc gactgctgcc
     9781 acggtgcccc acggacgttg tttctcggtc accattgccg ctcgggcgtt tcgcagtgcc
     9841 ttggcactct ttccgctggc catccctgcc ggacctcgtc ttccgtaccg cgcacatccc
     9901 tgacgttgcc gggcgccagt gtagggatgt cttgttggaa gagactcagc tagatggacc
     9961 aatggcggcg ccgaggcctg acaggctgcg atgagcagca ggcgtcatgg cgcccatccc
    10021 agatgatgag aagcggataa gggcttgctg agcagcgaag actcgactct agaatccatc
    10081 aggtgaacgc cgacgagctg acggagctgg ttcgggtcgc cgcgttgcgc gtggcatccg
    10141 accgggggct cgacgcctcg gcgctgccga cgacggttcg gctgcgacgc ggttcggcaa
    10201 ccgacggcga ttacacgacc accctggcgt tgcaggcggc ccggccgttg ggcgtcgagg
    10261 cgcaggcatt ggcggactgg cttgccgagg cccttgcgag gtgccccgga atccgatcgg
    10321 cgacggctgc gggccccggc ttcgtcaatc tcgtcgtcga gccacccacg ggtcgtgacc
    10381 tgctgcacga catcgtggcg gggggcgccg aata
//
