LOCUS       NZ_CP014859            10393 bp    DNA     circular CON 20-NOV-2024
DEFINITION  Actinoalloteichus hymeniacidonis strain HPA177 chromosome, complete
            genome.
ACCESSION   NZ_CP014859
VERSION     NZ_CP014859.1
KEYWORDS    RefSeq.
SOURCE      Actinoalloteichus hymeniacidonis
  ORGANISM  Actinoalloteichus hymeniacidonis
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Pseudonocardiales; Pseudonocardiaceae; Actinoalloteichus.
REFERENCE   1  (bases 1 to 6306386)
  AUTHORS   Schaffert,L., Albersmeier,A., Winkler,A., Kalinowski,J.,
            Zotchev,S.B. and Ruckert,C.
  TITLE     Complete genome sequence of the actinomycete Actinoalloteichus
            hymeniacidonis type strain HPA 177T isolated from a marine sponge
  JOURNAL   Stand Genomic Sci 11, 91 (2016)
   PUBMED   28031775
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6306386)
  AUTHORS   Schaffert,L., Albersmeier,A., Winkler,A., Kalinowski,J., Zotchev,S.
            and Ruckert,C.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAR-2016) CeBiTec, Bielefeld University, Sequenz 1,
            Bielefeld, NRW 33615, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Newbler v. 2.8
            Genome Coverage                   :: 159x
            Sequencing Technology             :: Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_001747425.1-RS_2024_11_18
            Annotation Date                   :: 11/18/2024 01:17:00
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,434
            CDSs (total)                      :: 5,374
            Genes (coding)                    :: 5,337
            CDSs (with protein)               :: 5,337
            Genes (RNA)                       :: 60
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 48
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 3 of 37
            Pseudo Genes (incomplete)         :: 34 of 37
            Pseudo Genes (internal stop)      :: 1 of 37
            Pseudo Genes (multiple problems)  :: 1 of 37
            CRISPR Arrays                     :: 13
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 14:46:53
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 409211
            Orig. end                         :: 419604
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP014859.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10393
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="ectoine"
                     /region_number="1"
                     /rules="ectoine_synt"
                     /tool="antismash"
     cand_cluster    1..10393
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="ectoine_synt"
                     /kind="single"
                     /product="ectoine"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10393
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5393](+)"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5393
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
     misc_feature    242..254
                     /note="TFBS match to MatP, Cell cycle regulator,
                     confidence: weak, score: 19.11"
                     /tool="antismash"
     misc_feature    complement(771..785)
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: weak, score: 18.0"
                     /tool="antismash"
     misc_feature    complement(1039..1053)
                     /note="TFBS match to MexT, Global virulence regulator,
                     confidence: medium, score: 18.79"
                     /tool="antismash"
     tRNA            1443..1515
                     /anticodon=(pos:410687..410689,aa:Thr,seq:tgt)
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /locus_tag="TL08_RS01925"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /old_locus_tag="TL08_t01980"
                     /product="tRNA-Thr"
     gene            1443..1515
                     /locus_tag="TL08_RS01925"
                     /old_locus_tag="TL08_t01980"
     misc_feature    complement(1470..1483)
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 15.54"
                     /tool="antismash"
     gene            1783..2766
                     /gene="mgrA"
                     /locus_tag="TL08_RS01930"
                     /old_locus_tag="TL08_01985"
     CDS             1783..2766
                     /EC_number="1.1.1.-"
                     /GO_function="GO:0047834 - D-threo-aldose 1-dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /gene="mgrA"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1039: aldo/keto reductase family oxidoreductase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009945347.1"
                     /locus_tag="TL08_RS01930"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="TL08_01985"
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="WP_069846129.1"
                     /transl_table=11
                     /translation="MSYQAADRRYDDMVYRRTGRSGLLLPAISLGLWQNFGADRPYETG
                     RAVARRAFDLGITHFDLANNYGPPYGAAETTFGRLLADDLRPYRDELIVSTKAGYDMWP
                     GPYGNWGSRKYLLASLDQSLARTGLDYVDIFYSHRFDPDTPLDETVGALISAYQQGKAL
                     YVGVSNYSPVATREATDVLRRAGIPLLVHQPSYSILNRWIEPDLLAAIGELGVGCVVFS
                     PLAQGLLTDRYLRGVPAGSRAARNEGLADTLTSEQLDRLRRLDEVASARDQTLAQLALS
                     WVLRDPRVTSALIGASSVAQLEQNVGALAAPPLTDAELAEIDSIVA"
     misc_feature    2801..2818
                     /note="TFBS match to ArcA, Repressor of carbon oxidation,
                     confidence: weak, score: 16.42"
                     /tool="antismash"
     gene            2950..3495
                     /gene="ectA"
                     /locus_tag="TL08_RS01935"
                     /old_locus_tag="TL08_01990"
     CDS             2950..3495
                     /EC_number="2.3.1.178"
                     /GO_function="GO:0033816 - diaminobutyrate
                     acetyltransferase activity [Evidence IEA]"
                     /GO_process="GO:0019491 - ectoine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="ectA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005441530.1"
                     /locus_tag="TL08_RS01935"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="TL08_01990"
                     /product="diaminobutyrate acetyltransferase"
                     /protein_id="WP_069853184.1"
                     /transl_table=11
                     /translation="MTKAHQEPPNDESGSGTRVVIEVPARTDGAAMWRIARDSGSLDLN
                     SPYAYVLWGRDFAATSVVARIGDRVGGFITGYLRPEAPEVLMVWQVAVDAEHRGQGLAG
                     RMLATLVDRAIALGARFVETTITADNAASIRLFTGLARDRDTTIERSDLFAAELLGDGH
                     AAEDLYRIGPFGTVGSTL"
     misc_feature    3490..3492
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            3649..4905
                     /gene="ectB"
                     /locus_tag="TL08_RS01940"
                     /old_locus_tag="TL08_01995"
     CDS             3649..4905
                     /EC_number="2.6.1.76"
                     /GO_function="GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /GO_function="GO:0047307 - diaminobutyrate-pyruvate
                     transaminase activity [Evidence IEA]"
                     /GO_process="GO:0019491 - ectoine biosynthetic process
                     [Evidence IEA]"
                     /NRPS_PKS="Domain: Aminotran_3 (21-355). E-value: 4.2e-72.
                     Score: 234.9. Matches aSDomain:
                     nrpspksdomains_TL08_RS01940_Aminotran_3.1"
                     /codon_start=1
                     /gene="ectB"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_3"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1013: aminotransferase class-III"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009947388.1"
                     /locus_tag="TL08_RS01940"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="TL08_01995"
                     /product="diaminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="WP_172803717.1"
                     /sec_met_domain="Aminotran_3 (E-value: 1.1e-73, bitscore:
                     246.5, seeds: 13, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNVFETLESEVRSYSRSWPTVFDRANGSWLHDESGKPYLDFFAGA
                     GALNYGHNNPILKRALIEYIERDGVTHSLDMGTVAKRDFLNTFKQVVLEPRGLDYKVQF
                     PGPTGANSVESALKLVRKVTGRESVINFTNAFHGMTLGALSVTGNSMKRGGAGIPLVHA
                     TPMPYDNFLDGQTPDFLWLERLLEDSGSGLNEPAAVIVETLQGEGGINVARVEWLRALS
                     ELCKRHGMLLIVDDVQMGCGRTGPFFSFEAAGIVPDIVCLSKSIGGYGLPMALTLLRPE
                     LDVWEPGEHNGTFRGNNPAFITAAAALQEYWTSDLLEKGTLAKGEHIGRALESLAETIP
                     GLEARGRGLARGLAFDDAEQAGQVCRAAFDRGLLMETSGPESEVAKLMPALTITDEEVD
                     KGLAIVTDAVHAVCGTKSS"
     aSDomain        3712..4713
                     /aSDomain="Aminotran_3"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_TL08_RS01940_Aminotran_3.1"
                     /evalue="4.20E-72"
                     /label="TL08_RS01940_Aminotran_3.1"
                     /locus_tag="TL08_RS01940"
                     /protein_end="355"
                     /protein_start="21"
                     /score="234.9"
                     /tool="antismash"
                     /translation="FDRANGSWLHDESGKPYLDFFAGAGALNYGHNNPILKRALIEYIE
                     RDGVTHSLDMGTVAKRDFLNTFKQVVLEPRGLDYKVQFPGPTGANSVESALKLVRKVTG
                     RESVINFTNAFHGMTLGALSVTGNSMKRGGAGIPLVHATPMPYDNFLDGQTPDFLWLER
                     LLEDSGSGLNEPAAVIVETLQGEGGINVARVEWLRALSELCKRHGMLLIVDDVQMGCGR
                     TGPFFSFEAAGIVPDIVCLSKSIGGYGLPMALTLLRPELDVWEPGEHNGTFRGNNPAFI
                     TAAAALQEYWTSDLLEKGTLAKGEHIGRALESLAETIPGLEARGRGLARGLAF"
     gene            5001..5393
                     /locus_tag="TL08_RS01945"
                     /old_locus_tag="TL08_02000"
     CDS             5001..5393
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     ectoine: ectoine_synt"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005165143.1"
                     /locus_tag="TL08_RS01945"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="TL08_02000"
                     /product="ectoine synthase"
                     /protein_id="WP_069846132.1"
                     /sec_met_domain="ectoine_synt (E-value: 4e-53, bitscore:
                     176.9, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIVRSLGEIEETDRDIKTPNWRSKRIVLAKEGVGFSVHETTIYAG
                     TVNDFWYANHVEAVFVTEGHGELTDKETGEVYKLAPGTLYLLDGNERHQVRAETEIKTV
                     CVFNPPVTGAEVHDENGVYPLIVEPV"
     gene            5620..6522
                     /gene="thpD"
                     /locus_tag="TL08_RS01950"
                     /old_locus_tag="TL08_02005"
     CDS             5620..6522
                     /EC_number="1.14.11.55"
                     /GO_function="GO:0016706 - 2-oxoglutarate-dependent
                     dioxygenase activity [Evidence IEA]"
                     /codon_start=1
                     /gene="thpD"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PhyH"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019806918.1"
                     /locus_tag="TL08_RS01950"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="TL08_02005"
                     /product="ectoine hydroxylase"
                     /protein_id="WP_069846134.1"
                     /sec_met_domain="PhyH (E-value: 1.4e-31, bitscore: 108.7,
                     seeds: 39, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTVVDVETGDLYPTRVGDKPSLISRQDPVVWPSNESGPIDQRTLA
                     SYDEKGYLTVDGLLTPAEVQQFWQELMDLTNDAGVKADERTIVEKSSDEVRSIFEVHKI
                     SSAIGKLATDPRVLDRARQIVGSDVYIHQSRVNLMPGYRGTGFYWHSDFETWHAEDGMP
                     RMRAVSMSIALTDNYPFNGGLMLMPGAHRTFVSCVGETPEDNYKASLKAQKIGVPDEDS
                     ITKLAAEHGIDQFTGSAGSALMFDSNSMHGSGNNITPFPRSNIFLVFNSVENTLQDPYG
                     AAAPRPTFVADRAFVPVQR"
     gene            complement(6595..7695)
                     /locus_tag="TL08_RS01955"
                     /old_locus_tag="TL08_02010"
     CDS             complement(6595..7695)
                     /EC_number="3.2.2.-"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016700307.1"
                     /locus_tag="TL08_RS01955"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="TL08_02010"
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="WP_236750457.1"
                     /transl_table=11
                     /translation="MTAAAQPTVVDRAAGCLLAGAMGDSLGAPVEFSRLAEIRQAYGPD
                     GIVELPKPGLITDDTQLTLFTAEGYISAWVRGKHLQTWNPAQEVWKAYRRWLSTQQGRG
                     PGDNPYGLLAERRLYANRSPGLATLRALAHEQPPTDDIQYETANGCGGVVRTAPAGFAP
                     TGELAYRMGCQFAALTHGHPEGWAPAGSLALLVHLLAVRRRTLSDAVDQVTGRVLRDDP
                     TTASLLAAAVKAAERDNEDARAAREARAFGKPPHTGGPSADSIAELGAGWIAPETLAIA
                     VYSALTHRKSAQFPDALRLAANHSGDSDSTAALTGNILGALHGTAVLPKPWLARLELAD
                     VIADIGYDLGATCAGEEFEEGRYLVA"
     misc_feature    8152..8168
                     /note="TFBS match to NuR, Nickel-responsive regulator,
                     confidence: weak, score: 21.09"
                     /tool="antismash"
     misc_feature    8174..8187
                     /note="TFBS match to ArgR, Regulator of arginine
                     biosynthesis genes, confidence: weak, score: 16.58"
                     /tool="antismash"
     misc_feature    complement(8664..8678)
                     /note="TFBS match to VqsM, Regulator of quorum-sensing
                     signalling systems, confidence: medium, score: 17.8"
                     /tool="antismash"
     misc_feature    9025..9041
                     /note="TFBS match to RicR, Copper-responsive regulator,
                     confidence: weak, score: 21.62"
                     /tool="antismash"
     misc_feature    complement(9072..9087)
                     /note="TFBS match to AfsQ1, Two-component system AfsQ1-Q2,
                     activator of antibiotic production, confidence: medium,
                     score: 17.28"
                     /tool="antismash"
     misc_feature    complement(9113..9126)
                     /note="TFBS match to ANR, Anaerobic transcriptional
                     regulator, confidence: weak, score: 18.84"
                     /tool="antismash"
     misc_feature    complement(9612..9626)
                     /note="TFBS match to MexT, Global virulence regulator,
                     confidence: weak, score: 17.51"
                     /tool="antismash"
ORIGIN
        1 cgcacccgcc gcggccgcta ccagcaacga acgcaacggt gcgtaactgg cgagcgcgaa
       61 acacgcggca gtggcggctg cggcggtgat catgagcggt agatcgccga ccaccggtgg
      121 aaggcgttcg gccacggccg attcggaggc gccgagggcc accccgatcc gcagcgcgag
      181 cacgacgccg gtgaccagac ccgccgtcat caacagcgtc tcgcccgccc ggccggtggc
      241 ggtgacgttg tatccgctga ccgcgtcctg cgcgacaccg acgaccgaga gcccggacaa
      301 caggacgacg aggttggcgc ccaccgcgag cgaggtcgtc gcatcgccga ggagatccga
      361 ctcgtagacg gccatcgcgg cgccggtggc gatggccccg ccgaccacct gttggaagaa
      421 gaacgggagc gctcgcctat tgagctgtcg cccgatctgg tcgatcacga tcgtgatcag
      481 cgcggccagc atcaccagga acggacccgc accgatcaag acggcgatgg ccgccgccat
      541 tccaccccag gccagcgtcg agacccagcg cgggtagggg tgcctggcgg tggtgatccg
      601 ctgcagttcg gagtgggcgt cgccgacgct gatctcaccc gaggtgatcc gccggaccag
      661 gtcctcggtg tccgagagcc tgctgtagtc catggacctg ctgcgcacca cccgcagcgt
      721 cgtgatcggc gcctggtcga caccgaggtg acagcacacg gtgatcgagg tgtagatgac
      781 gtccacctcg cagtgcggca gaccgaaggc gttggtcacc gcgaggatcg tcgcggtggc
      841 gtccgccgca cccgctccgc tggccatctg gacctcgccg atccgcagcg cgagatcgag
      901 caccagatgg atcgtcgggg catcgggaag ggcaggcccg tattcgatgt gcgcgtcggt
      961 gaccggcgcc gggttcttgc ggcgcagtac gtcccgagcc cgctgggcga gacccacggg
     1021 ccttcctcct gttccttgat cgccgtggtt gatggtggtg cggcaggccg gtggtgcgcc
     1081 ctgcggcctg cccggggcgt acgggttagc cgcccgggtc atcggcgcgg atgacgtcgc
     1141 cgttgcaggt gctcggtagt ggcgttggac acgtttcgtc ggtcgtggtc ctctgtgatc
     1201 ctccgccgga ggaccgcgtg ctgaccacgg taggcttcgg ggcagaacgc acaggtcaga
     1261 gaccttgttc ccacactgat aggtgacgcg cgactcacgg gtcgcctgcg aggattccca
     1321 cgcgggcggg cggcgtgtcg cgaccggtgc cttgggtgct gtcccacccc cggcttagga
     1381 tgtgggcgac cgctgcgcgc ggggcgagtc gatcgtccga gcacggtgcg cagacgggca
     1441 acgccgacgt agctcaactg gcagagcact tgtcttgtaa acaagaggtc aggggttcga
     1501 gccccctcgt cggctcagcg ttgatcagca cgaacggccc cggtggtatt ccgccggggc
     1561 cgttcgggtc tcggttcggg gcgcgtctgg cgagtaacgg atggcggggc cgggcgatgc
     1621 cacgcagtgg gaagcaccat gcgcggtccg cggctggatg cgaggctcga ctctgatccg
     1681 gtccaagatc cgcttggtcg cgacgatgac gacggcttca cccggcccgt ggtagaaccc
     1741 acgaaatctc gacctgcgcc cgccgaactg agaggaaccc gcatgtccta tcaggcggcc
     1801 gaccggcgtt acgacgacat ggtctaccgg cggaccggtc gaagcgggct gctgttgccc
     1861 gcgatctcgc tcgggctgtg gcagaacttc ggcgcggacc ggccgtacga gaccggccga
     1921 gcggtggctc gccgggcctt cgacctcggc atcacccact tcgacctcgc caacaactac
     1981 ggaccgccct acggcgccgc ggagacgacc ttcgggcgcc tgctggccga cgacctgcgg
     2041 ccgtaccggg acgagctgat cgtctcgacc aaggcgggct acgacatgtg gcccggcccc
     2101 tacggcaact gggggtcgcg caagtatctg ttggcctcgt tggaccaatc gctggcgcgc
     2161 accggcctgg actacgtcga catcttctat tcgcaccggt tcgacccgga cacgccgctg
     2221 gatgagaccg ttggcgcgtt gatttcggcg tatcaacagg gcaaggcgtt gtatgtcggt
     2281 gtttcaaatt attccccggt ggctacccga gaggcaacgg acgtgttgcg tcgggcgggt
     2341 attccattgc ttgttcacca accgtcgtac tcgatattga atcggtggat cgaacccgat
     2401 ctgctggccg ccatcggcga actcggggtt ggttgtgtgg ttttctcacc attggcgcag
     2461 ggattgctca ccgaccgcta tctgcgcggc gtcccggcgg gctctcgggc cgcgaggaac
     2521 gaggggctgg cggacacgct gaccagcgaa caactcgatc ggctgcgcag gctcgacgag
     2581 gtggcctcgg ctcgggacca gaccctggcc cagctggcgc tcagctgggt gctgcgcgac
     2641 ccgagggtga cgtcggccct gatcggggcc agctcggtgg ctcagctgga gcagaacgtc
     2701 ggagcgctcg ctgccccgcc gctgaccgac gcggagctag ccgagatcga ctcgatcgtc
     2761 gcctgagttc accctttcga ggggtgcctg ccgagtcgat ttgttgatgt gctattaaca
     2821 ccggcttgtc gccagaagca acctttttgt tgtacttgtc accgggtttt ctgagaagct
     2881 cctggtagat gagtgttcgg agtgtcgttg acctggcact tttcggtggc tctgttacgt
     2941 tggctcatca tgaccaaggc tcatcaagaa ccaccgaatg atgaaagcgg gtcaggtacg
     3001 cgtgtggtga tcgaggtgcc tgcacggacc gatggcgccg cgatgtggag aattgcgcgt
     3061 gattccggct cgctcgacct gaactcccct tacgcctatg tgctctgggg acgagacttc
     3121 gcagccacct cggtggtggc gcgcatcggg gaccgtgtcg gcggcttcat aactgggtac
     3181 cttcgaccgg aagctccgga ggttctcatg gtgtggcagg tggcggtcga cgcggaacat
     3241 cgtggtcagg gcttggccgg tcggatgctg gccaccttgg tggaccgagc gatcgcgctg
     3301 ggggcccgct tcgtggagac caccatcacg gcggacaacg ctgcctcgat ccggttgttc
     3361 accggtctgg cgcgcgaccg cgacaccacc atcgagcggt cggatctctt cgccgcggaa
     3421 ttgctcggcg acggccacgc tgccgaggat ctttaccgaa ttggcccatt cggaacggtt
     3481 gggtcgacct tatagacctt ttcgcaacca agggcttgtt ggagttcgca taagcggact
     3541 tctcagccgg gagtggcgtt gctgcgtatt gtcggggatc gatcttttcc tggttcagtg
     3601 ccgtcgcgtc ggttggtaaa acgttgacca tagatcggac ggagtatcgt gaacgtcttc
     3661 gaaaccttgg aatccgaggt tcgtagctac tcgaggagct ggcccacggt tttcgaccgg
     3721 gctaacggaa gctggctgca tgacgagtcg ggaaaaccct atcttgactt cttcgcaggc
     3781 gccggggcgt tgaactacgg ccacaacaac ccgatcttga agcgagcctt gatcgaatac
     3841 atcgagcgcg acggggtcac ccacagtctc gatatgggca ccgtggcgaa acgtgacttc
     3901 ttgaacactt tcaaacaggt ggtgctcgag ccgcgcggtc tcgactacaa ggtccagttc
     3961 cccggaccga cgggtgccaa ctcggtggag tctgccctga aactggttcg caaggtcacc
     4021 ggtcgcgaat ccgtcatcaa cttcacgaat gcatttcacg gtatgacgct gggtgccctc
     4081 tcggtgaccg gcaactcgat gaagcgtggc ggcgcgggca tcccgctggt gcacgccacc
     4141 ccgatgccct acgacaactt cctcgacggc cagacccccg acttcctgtg gttggagcgg
     4201 ctgctggagg acagcggttc cgggctcaac gagcccgctg ccgtgatcgt cgagacgctg
     4261 cagggcgagg gcgggatcaa cgtcgcgcgg gtcgagtggc tgcgcgcgct gtccgagctg
     4321 tgcaagcggc acggaatgct gctcatcgtc gacgacgtgc agatgggctg tggccgaacc
     4381 gggccgttct tcagcttcga ggccgcgggc atcgttcccg acatcgtctg cctgtcgaag
     4441 tccatcggcg gctacggcct gccgatggcg ctgacactgc tgcgccccga gctcgacgtc
     4501 tgggagccgg gcgagcacaa cggcaccttc cgaggcaaca acccggcctt catcaccgcc
     4561 gccgccgcgc tgcaggagta ctggaccagc gacctgctgg agaagggcac cctagccaag
     4621 ggcgagcaca tcggccgggc cctcgaatcg ctcgcggaga ccattcccgg tctggaagcc
     4681 cgaggccgag gcctggcgcg tgggctcgcc ttcgacgacg cggagcaggc aggccaggtg
     4741 tgccgggcgg ccttcgaccg cggcctgctg atggagacct ccggcccgga gagcgaggtc
     4801 gccaagctga tgcccgcgct caccatcacc gacgaagagg tcgacaaggg cctggccatc
     4861 gtcaccgacg ccgtccacgc ggtctgtggg acgaagtcga gctgacccgt cttcgtgggc
     4921 tcgccggcgc cggtccgata ccggcccggt gaccggggag cccattgatc caattcgatt
     4981 caactgctag gagtgaccct gtgatcgttc gttcgctcgg tgagatcgag gagaccgacc
     5041 gggacatcaa gacgcccaac tggcgcagca agcgcatcgt gctggcgaaa gagggcgtcg
     5101 ggttctcggt ccacgagacc accatctacg cgggcacggt gaacgacttc tggtacgcca
     5161 accacgtcga ggcggtgttc gtcaccgagg gacacggcga gctgaccgac aaggagaccg
     5221 gcgaggtcta caagttggcg cccggcacgc tctacctgct cgacggcaac gagcggcacc
     5281 aggtccgggc cgagaccgag atcaagaccg tgtgcgtgtt caacccgccg gtgaccggcg
     5341 ccgaggtgca cgacgagaac ggggtctacc cgctgatcgt cgagcccgtc tagttcggcg
     5401 tcaccggccg aagacccgcc agcaggcccg atcgggcctg ctggcgtagt agtccgacca
     5461 cacagcagga ggcgattgcc caggaacctc gaagggtatg gattccggta ggagaccgga
     5521 catgccgcag acccgaaccc accacgaacc aagagagcgg ctcgtgcaga ccatcgcgcg
     5581 ggccggtgtc ggcaattcgc cacggagatt ggagagcgca tgactgtcgt cgacgtcgag
     5641 accggggatc tctaccccac ccgagtgggt gataaaccat cgctcatcag ccggcaggac
     5701 ccggtggtat ggccgagcaa cgagtcgggc ccgatcgatc agcggacgct cgcgagctac
     5761 gacgagaagg gctacctgac ggtcgacggc ctgctcaccc cggccgaggt tcagcagttc
     5821 tggcaggagc tgatggatct caccaacgac gcaggcgtca aggcagacga gcggaccatc
     5881 gtcgagaaga gttctgacga ggtcagatcc atcttcgagg tgcacaagat cagctcggcg
     5941 atcggcaagc tcgccaccga tccgagggtg ctggaccggg ctcggcagat cgtcggttcc
     6001 gatgtctaca tccatcagag ccgggtcaac ctgatgccgg ggtatcgagg tacgggcttc
     6061 tactggcact cggacttcga gacctggcac gccgaggacg gcatgcctcg gatgcgcgcg
     6121 gtcagcatgt cgatcgcgct caccgacaac tacccgttca acggcgggct catgttgatg
     6181 cccggtgcgc atcggacctt cgtgtcctgc gtcggcgaga cgcccgagga caactacaag
     6241 gcctcgctca aggcacagaa gatcggtgtt cccgacgagg acagcatcac caagctggct
     6301 gcggaacacg gcatcgacca gttcaccggc tcggctggtt cggccttgat gttcgactcg
     6361 aactccatgc acggttcggg caacaacatc acgccgttcc cgaggtcgaa catcttcctg
     6421 gtgttcaaca gcgtggagaa cacgctgcaa gacccgtacg gtgcggccgc cccgcgaccg
     6481 acgttcgtgg cggaccgggc cttcgttccc gtccagcgct gacgccgacg gacgacgcgg
     6541 gtcgcctggg gccgcccgcg tcgtccgggt ctgagaaggc gatcccgctg cggctcacgc
     6601 caccaggtag cggccctcct cgaattcctc gcccgcgcag gtcgccccga ggtcgtatcc
     6661 gatgtccgcg atcacatcgg ccaactccag ccgggccagc catggcttgg gcaacaccgc
     6721 ggtgccgtgc agcgcgccga ggatgttccc ggtgagcgcc gccgtcgaat cgctgtcgcc
     6781 cgagtggttg gccgccagcc gcagggcgtc cgggaactgg gcggacttgc gatgggtcaa
     6841 cgccgagtac acggcgatgg ccagcgtctc cggtgcgatc caacccgcgc cgagttccgc
     6901 gatggagtcg gcggagggcc ctcccgtgtg cggtggcttc ccgaaggccc gggcctccct
     6961 ggcagccctg gcgtcctcgt tgtcgcgttc ggcggccttg accgccgccg ccagcaggct
     7021 cgcggtggtc gggtcgtcgc gcagcacgcg gccggtcacc tgatcgaccg catcgctgag
     7081 cgtgcgacgg cgcaccgcca gcaggtgcac caacagcgcg agcgacccgg cgggcgccca
     7141 gccctcgggg tgtccatggg tcagggcggc gaactggcag cccatccgat aggccagttc
     7201 gccggtcggc gcgaacccgg cgggcgcggt gcgcaccacc ccgccgcagc cgttcgccgt
     7261 ctcgtactgg atatcgtcgg tcggcggctg ctcgtgggcc aacgcccgca gcgtggccag
     7321 tcccggcgag cggttggcgt agagcctgcg ctcggccagc aggccatagg ggttgtcgcc
     7381 gggccccctg ccctgctgtg tcgacagcca gcgccggtac gccttccaca cctcctgcgc
     7441 cggattccag gtctgcaggt gcttacctcg gacccacgcg ctgatatagc cctcggcggt
     7501 gaacagggtg agctgggtgt cgtcggtgat gaggccgggt ttgggcagtt cgacgatccc
     7561 atccgggccg taggcctggc ggatctcggc gaggcgggag aactccaccg gggctcccag
     7621 cgagtcgccc atggcaccgg ccaacagaca gcccgccgca cggtccacca cggtgggctg
     7681 tgctgctgct gtcacgttct cctccatgcg ctgccaatgc ccaccgccgc gatgtacgcg
     7741 ggacacgaca gtagcctcta cccaacgggt gaatcgcggt tcgtcggaat ggtcgagatc
     7801 cgaccggtgc ggacgtcggt catgctcgcg ggcggcgccg acgcggctcg acgaccaaga
     7861 ttgcgattgc acctttcccc accaggatca ggcgagcacg atctaggttg gcgaggttcg
     7921 acgaacgacg tcgacacgaa caggtcgagg aggcaggccg tccatggacc ctgggacgcc
     7981 gcctgtcggc gcgaaccgcc tcgaattccg cctgttaggg gcgttcgagg tgctcagttg
     8041 cggacggccg atcgtgctcg gcagtcgggc gatgcgcagg ctgatggcgt tgttgacgct
     8101 caatgccaat caggtcgtgt ccgtcgaccg gatcatcaag gtgctctggg acggcgaacc
     8161 acccgcaact gtacgcacga tcttgcaggg atatgtgtca cgcctacgta aactgatcgt
     8221 cggtgcggcc ggggacgtcg gcgatgttgc gatcatcacc cgctcgcccg gctatctgct
     8281 gcggatcgac cccggccgga tcgatctgca ccgggcgcgg gagctggcca agaaggccgc
     8341 gctgctgacc ggccgttccc gcgcggtggc gttgcgagcg gcgctggagt tgtggcgggg
     8401 tcacaccctg gccgacatgg acgatctgcc cgccctgcga gccatcgcga tgtcgatcga
     8461 ggaactccgg ctcagcctgg tcgaggaccg gatcgccgcc gaactggccg aggggccgag
     8521 aaccggcctg gtcaccgagt tggacgaatt ggtcgcggcg cacccgctgc gcgaacgcct
     8581 caccgaccaa ctcatgatgg cgctgcatca caccgggcgg cgcgccgatg cactgcggcg
     8641 ctaccacgca ctgcgcgagc ggttggccga ggaactcggc gtcgatccgt ccccgcagct
     8701 gcagctgacg tatcaacgga ttctgcagga cgaggccacc gtggtggccc cggagacagc
     8761 cgagcccgcg tcgaccgatt cgaacgagcc cgccgtcgtg cagcccgtgg cagggcacac
     8821 ccaggtggtg cccgcgcaac tcccgccccg gatcgggggc ttcgtgggca gggacgagga
     8881 gttcatcgtc ctggactcgc tgcggccgag caggcgcgac gagggcggat ccacgatcgc
     8941 cgtggtcagc ggcgaggccg gcctcggtaa gagcgctctg gccgtgaact gggcgcatcg
     9001 ggtgagcaac ggccccgaca gcgaataccc ggatgggcaa ctcttcgcct cgctgcgcgc
     9061 cgtcgacccc tgtgacccac cggtgtcgcc tgcggacgtg ttgagacagt tcttgatcgg
     9121 cctcggcctg acctccgaac agattcccac cgggttgacc gaacggacgg cgctgtaccg
     9181 ctcgatcacg gcgaaacggc ggatgctcgt gctgctcgac gacgccgtcg accacctgca
     9241 ggtgtcgcca ctgctgcccg cctcgccgtc ttgcacggtg gtgatcacca gcagacggat
     9301 gctcgacggc ctgctgatca ccaatggcgc ccggctgatc cgactcggtc cgctgtccgc
     9361 cgcccaggcg ggtgagctga tcaaacgcct cgccgtcggg gtcgagccga ccgcgagcga
     9421 cctggaccgg ctgaccgagc tgtgcggcaa tctgccgctg gccctgcgga tcgccgctgc
     9481 ccggatgacg ggcgacccgg aactctccat cggcgatctg atcgacgaac tggccgacga
     9541 gcgccgtcgg ctcggagcac tgcgggcggg cgacccgcag atcagcgtcc gaggggcgct
     9601 cgaactcacc tatcgacggc tccgatccga cgccgcccgg ttgttccgcc tcatcggcgt
     9661 ccatcccggt gtggagctga gccgctccgt gttggcggcg atgtcgggca gcgccttcgc
     9721 ccaggtcgcc gagcaactgc acgtcctgga atcggtgcat ctgatctcgt cgcgtgagca
     9781 tggtcgattc ggcagtcacg acctcgtccg gctctacgcc agggaactcg ccaccgacga
     9841 actcgacgac gcggagcggc tcgccgccga gacggcgatg gtggacttct acttggccgt
     9901 cgccgaacgg gggcgggacc tgatccgttc caccccgggc agtccggggc cgtacgcacc
     9961 cggcttcgaa ctgccggaga cgacgagtct ggaccaggcc gtcggctggt tcgatcggga
    10021 gtggaacaac ctgatgcacg cggcccggac cgccgtccgc aggcagcggc acgaggcagc
    10081 ctggcgcatc gcgtggggcg ccgcgccgta cgtgctgttc cgacacctgc tggacgagtc
    10141 gaccgagttg ttcgatcagg ggctcgcctc ggcgcgaacc tcgggcgacc ggttcggcga
    10201 atgtctgatg ctgcgctgcc gcgtcaacgt cgccctctac cgctcggcca agtcggagga
    10261 ggcgctggag cacgcggtcg aagccctggc gatcgccgag gaactgggca agctggaaat
    10321 cctcgcacct gcggtgagca gtgtcttcac ctcgcaggcc aacctcggtc gctacgagga
    10381 cgcccggcag ggc
//
