LOCUS       NZ_CP017157            10990 bp    DNA     linear   CON 31-MAY-2024
DEFINITION  Streptomyces lydicus strain 103 chromosome, complete genome.
ACCESSION   NZ_CP017157
VERSION     NZ_CP017157.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces lydicus
  ORGANISM  Streptomyces lydicus
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 8201357)
  AUTHORS   Jia,N., Ding,M.-Z., Gao,F. and Yuan,Y.-J.
  TITLE     Complete genome sequencing of Streptomyces lydicus 103 and metabolic
            pathways analysis of antibiotic biosynthesis
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8201357)
  AUTHORS   Jia,N., Ding,M.-Z., Gao,F. and Yuan,Y.-J.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2016) Key Laboratory of Systems Bioengineering
            (Ministry of Education), Tianjin University, Tianjin 300072, PR
            China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SMRT Analysis v. 2.3.0
            Genome Coverage                   :: 100x
            Sequencing Technology             :: PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_001729485.1-RS_2024_05_31
            Annotation Date                   :: 05/31/2024 02:23:15
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,288
            CDSs (total)                      :: 7,195
            Genes (coding)                    :: 7,061
            CDSs (with protein)               :: 7,061
            Genes (RNA)                       :: 93
            rRNAs                             :: 7, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 47 of 134
            Pseudo Genes (incomplete)         :: 104 of 134
            Pseudo Genes (internal stop)      :: 11 of 134
            Pseudo Genes (multiple problems)  :: 26 of 134
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 01:52:31
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 6060070
            Orig. end                         :: 6071060
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP017157.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Source DNA is available from Prof. Ying-Jin Yuan.
            Annotation modified by submitter.
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10990
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="butyrolactone"
                     /region_number="26"
                     /rules="AfsA"
                     /tool="antismash"
     cand_cluster    1..10990
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="AfsA"
                     /kind="single"
                     /product="butyrolactone"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10990
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5990](+)"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5990
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
     gene            245..1096
                     /locus_tag="SL103_RS26325"
                     /old_locus_tag="SL103_26325"
     CDS             245..1096
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006602520.1"
                     /locus_tag="SL103_RS26325"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SL103_26325"
                     /product="PaaX family transcriptional regulator C-terminal
                     domain-containing protein"
                     /protein_id="WP_069571407.1"
                     /transl_table=11
                     /translation="MTDQQTQRTPRSLIVTFYGAYGRGGPGTAGPATGAVPVAALIRLL
                     GVLGVDPPSVRSAVSRLKRRGLLVPGRTDAGAAAYGLSEPARQLLEDGDRRIFGRPAAR
                     LSDGWVLAVFSVPEEERHKRHLLRSRLARLGFGTAAPGVWIAPAQLYEETRHTLERLQL
                     SPYVDLFTGTHAGFAPTADAVARWWDLDAVAAQHRAFLAEQEPVLRRWSRRRTVPPEAA
                     YHDYLLALDAWRRLPYADPGLPSPLLPEDWPGGRAAEVFGRLHGTLQDAGARYVREVTT
                     GP"
     gene            complement(1108..1899)
                     /locus_tag="SL103_RS26330"
                     /old_locus_tag="SL103_26330"
     CDS             complement(1108..1899)
                     /EC_number="3.-.-.-"
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) HAD_2"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1115: HAD-superfamily hydrolase, subfamily IA,
                     variant"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016574418.1"
                     /locus_tag="SL103_RS26330"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SL103_26330"
                     /product="HAD family hydrolase"
                     /protein_id="WP_069571408.1"
                     /sec_met_domain="HAD_2 (E-value: 4.7e-28, bitscore: 97.2,
                     seeds: 50, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRVATEVGERPARGARGAAGGETIRAAVFDLDGTLANTLPAISKL
                     LVKVASEQGSSVTARQAATAIGKPPGPAFGRLLGRPEDDERVQAAISRYRELFAYEVLC
                     QGPQLLFPGVTAGLSALRAHGVELAVATSKTTRSAEALLDVMGIRDLVGPVIGQDMVRR
                     GKPHPEMVLRAAGRLGVAPWQSAYVGDTVGDMRMAVTARMEPVAVTYGVGTFGELAAVA
                     GTRMCSSFKDVVSVIAAARPRAAAGMPHRPAVAMGGPARRR"
     misc_feature    complement(2064..2078)
                     /note="TFBS match to IolR, Regulator of the inositol
                     catabolism, confidence: medium, score: 20.04"
                     /tool="antismash"
     misc_feature    complement(2125..2141)
                     /note="TFBS match to ZuR_variant_2, Zinc-responsive
                     repressor, confidence: weak, score: 17.33"
                     /tool="antismash"
     misc_feature    2127..2139
                     /note="TFBS match to MatP, Cell cycle regulator,
                     confidence: weak, score: 20.49"
                     /tool="antismash"
     misc_feature    complement(2299..2312)
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 12.61"
                     /tool="antismash"
     gene            2352..2993
                     /locus_tag="SL103_RS26335"
                     /old_locus_tag="SL103_26335"
     CDS             2352..2993
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1057: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018088830.1"
                     /locus_tag="SL103_RS26335"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SL103_26335"
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="WP_069571409.1"
                     /transl_table=11
                     /translation="MPTQHRAIQSRQALIRSAAETFLEKGVPAAGMVEISRRARLSKGA
                     LYFHFTSKDDLTLAVRDAALATLQEIEDAFTRSPQPLTTAVQDFAVELFERVESDAVLR
                     AGLRLRPETAPGLGIYALEQRWYALFLDKAVTCGTGGLGRPAAHAPDAEPRRTAQLLTS
                     IVVGLLHLGSEDGTWWDKEAVVGLWSLLPAAPGRITAAGIPAPDRLPAAG"
     gene            complement(3042..4070)
                     /locus_tag="SL103_RS26340"
                     /old_locus_tag="SL103_26340"
     CDS             complement(3042..4070)
                     /NRPS_PKS="Domain: PKS_ER (29-340). E-value: 1.1e-18.
                     Score: 59.4. Matches aSDomain:
                     nrpspksdomains_SL103_RS26340_PKS_ER.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) ADH_zinc_N"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1028: crotonyl-CoA reductase / alcohol dehydrogenase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018088829.1"
                     /locus_tag="SL103_RS26340"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SL103_26340"
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="WP_069571410.1"
                     /sec_met_domain="ADH_zinc_N (E-value: 1.6e-16, bitscore:
                     59.2, seeds: 86, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTPTAVPDVPAVQREVRLAARAEGQLTTRHFTLAEVPVPQPGPGQ
                     VLVRTRVMAVTAAMRTQMTGAPLPMPSFVPGQALWGSAVGEVVAAPGGGFAPGELVHHP
                     YGWREFAVVDEGRLRRLAPDALPTPAAHLSQGATAWGALTRAVEVRPGDTVFVTGAAGG
                     VGSLAGQLARRLGAGRVVGSTGSERKAARLRAELGYDDTIVRGAGPIDRQLRRAAPDGI
                     DVLLDTVGGEQLTAALAAARPDARFALVGALATQLGGDGGAVAPVELDAGLIITRRVVL
                     RGFGLHAHPDLPQEWTKEFGQGLRDGSLVFPHALLKGIEQAPRALCELTQGHHIGAVLV
                     EL"
     aSDomain        complement(3051..3983)
                     /aSDomain="PKS_ER"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_SL103_RS26340_PKS_ER.1"
                     /evalue="1.10E-18"
                     /label="SL103_RS26340_PKS_ER.1"
                     /locus_tag="SL103_RS26340"
                     /protein_end="340"
                     /protein_start="29"
                     /score="59.4"
                     /tool="antismash"
                     /translation="HFTLAEVPVPQPGPGQVLVRTRVMAVTAAMRTQMTGAPLPMPSFV
                     PGQALWGSAVGEVVAAPGGGFAPGELVHHPYGWREFAVVDEGRLRRLAPDALPTPAAHL
                     SQGATAWGALTRAVEVRPGDTVFVTGAAGGVGSLAGQLARRLGAGRVVGSTGSERKAAR
                     LRAELGYDDTIVRGAGPIDRQLRRAAPDGIDVLLDTVGGEQLTAALAAARPDARFALVG
                     ALATQLGGDGGAVAPVELDAGLIITRRVVLRGFGLHAHPDLPQEWTKEFGQGLRDGSLV
                     FPHALLKGIEQAPRALCELTQGHHIGAVLV"
     CDS_motif       complement(3555..3617)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_SL103_RS26340_0001"
                     /evalue="1.50E-03"
                     /label="PKSI-ER_m5"
                     /locus_tag="SL103_RS26340"
                     /protein_end="172"
                     /protein_start="151"
                     /score="11.3"
                     /tool="antismash"
                     /translation="GDTVFVTGAAGGVGSLAGQLA"
     gene            4237..4854
                     /locus_tag="SL103_RS26345"
                     /old_locus_tag="SL103_26345"
     CDS             4237..4854
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1057: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006602525.1"
                     /locus_tag="SL103_RS26345"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SL103_26345"
                     /product="ScbR family autoregulator-binding transcription
                     factor"
                     /protein_id="WP_033269160.1"
                     /transl_table=11
                     /translation="MVKQERAVRTRRAVLEAAAHVIGTRGYQAATMAEIIQRAGVTKGA
                     VYFHFTSKDALARAVITEQTDPFLPQVSESRLQDAIDFTHQVALALRSDPLLQAGTRIA
                     VETTFSDEPLVPYQAWTDIITTMFSEARDNGELLPGVAPDRAAEFFVAAYMGVQLFSRA
                     ATNRADLPERVTALWKHTLPGLASPGAMSHLDPHGRAVQVSV"
     misc_feature    4645..4647
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     misc_feature    4891..4906
                     /note="TFBS match to CopR, Copper-responsive regulator,
                     confidence: weak, score: 16.79"
                     /tool="antismash"
     misc_feature    4892..4908
                     /note="TFBS match to ZuR_variant_2, Zinc-responsive
                     repressor, confidence: weak, score: 15.66"
                     /tool="antismash"
     gene            5001..5990
                     /locus_tag="SL103_RS26350"
                     /old_locus_tag="SL103_26350"
     CDS             5001..5990
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     butyrolactone: AfsA"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006602526.1"
                     /locus_tag="SL103_RS26350"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SL103_26350"
                     /product="ScbA/BarX family gamma-butyrolactone biosynthesis
                     protein"
                     /protein_id="WP_069571411.1"
                     /sec_met_domain="AfsA (E-value: 7e-23, bitscore: 79.2,
                     seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MASATLMPSWRDQRHDDRGRFADGNRPSALTTTVPREYVHRTAVS
                     EVFLTNWRRGTADSWVVSAQWPRAHSFYGPVGGLHDPLLLIETMRQAGILLSHVAHRVP
                     LDHPIIWQRVRYDLTPQALRAADEPAEVELHITDHDVLRRGKRLISARQHFRIRCDGAD
                     LATASLDYSCHSPAVYRRLRGKYSDLTLANAQRLPLPEPVTPRLVGRDRERDVVLSPTN
                     RPDRWQLRVDTSHPVLFDHPVDHAPGMLMIEAVRQAAQAAAPGLTLPTAMECDFERYAE
                     LDAPCWVQARAADGPRPAAADGGRQIEVSVEQHGKPVTAARITSVPTS"
     misc_feature    5250..5252
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     misc_feature    5283..5285
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            complement(6061..8379)
                     /locus_tag="SL103_RS26355"
                     /old_locus_tag="SL103_26355"
     CDS             complement(6061..8379)
                     /GO_function="GO:0003959 - NADPH dehydrogenase activity
                     [Evidence IEA]"
                     /GO_function="GO:0010181 - FMN binding [Evidence IEA]"
                     /GO_function="GO:0050661 - NADP binding [Evidence IEA]"
                     /GO_function="GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1050: monooxygenase FAD-binding"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018088826.1"
                     /locus_tag="SL103_RS26355"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SL103_26355"
                     /product="bifunctional salicylyl-CoA
                     5-hydroxylase/oxidoreductase"
                     /protein_id="WP_069571412.1"
                     /transl_table=11
                     /translation="MRVAVIGGGPGGLYAAVLLKRLDPTREITVWERNAPDDTFGFGVV
                     LSDETLGGIEHADPEVYRALQREFVRWDDIDIVHRGHTLTSGGHGFAALGRRRLLGILH
                     RRCRDLGIELRFRTEAPPAAELARTHDLVIAADGVHSATRTAHADAFGPRLTTHRCRYI
                     WLAADFALDAFRFEIAETAHGVVQLHAYPYSRPAPDSRPGAAPAGPRHPGGASTVIVEM
                     REEVWRAAGLDRADERESAEWCAKVFADALSGRPLRSNNSSWIAFRTVVNERWSHGNTV
                     LLGDAAHTAHFSIGSGTKLAVEDALALAACLQEQPDTAAALAAYEAERRPVVASTQRAA
                     RASLAWFEELATYVDQPPRQFAFNLLTRSRRVTHDNLRLRDPGFVAAVEDEAAVPAGTP
                     PMFTPFRLRGLTLRNRVVVSPMDMYSAQDGVPGDFHLVHLGARALGGAGLVMTEMVCVS
                     ERGRITPGCAGLYTPEQAAAWRRVTDFVHAQAPGAAIGLQLGHAGRKGSTKLMWDGIDE
                     PLDDGNWPLVAPSPLPYKPGSQRPQELTPDELDGIREQFAAAARRAVDCGFDLLELHCA
                     HGYLLSGFLSPLTNQRTDAYGGSLSRRLRFPLEVFDTVRALWPADKPMTVRISATDWAD
                     GGTTAEDAVTVAAAFAEHGADAIDVSTGQVVADERPDFGRSYQTPFADRIRNSLGVPVI
                     AVGAISSWDDVNSLVLAGRADLCALARPHLYDPHWTLHAAAEQGYAGQGAPWPLPYQAG
                     SRTPPTGRTDAPKPRLTLR"
     gene            complement(8382..9209)
                     /locus_tag="SL103_RS26360"
                     /old_locus_tag="SL103_26360"
     CDS             complement(8382..9209)
                     /NRPS_PKS="Domain: ECH (19-267). E-value: 1.1e-50. Score:
                     164.2. Matches aSDomain:
                     nrpspksdomains_SL103_RS26360_ECH.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1023: enoyl-CoA hydratase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004982203.1"
                     /locus_tag="SL103_RS26360"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SL103_26360"
                     /product="enoyl-CoA hydratase family protein"
                     /protein_id="WP_069571413.1"
                     /transl_table=11
                     /translation="MSPFAGSAHRTDPWQHIRVTRQDGVATVTLARPEKLNALTFEAYA
                     DLRDLLAELSRERSVRALVLAGEGRGFCSGGDVEEIIGATLGMDTGQLLDFNRMTGQVV
                     RALRECPFPVIAAVHGVAAGAGAVLALAADFRVADPSARFAFLFTRVGLSGGDMGAAYL
                     LPRVIGLGHATRLLMLGDTVRAPEAERLGLISELAEEGRADEAAAALARRLAEGPALAH
                     AQTKALLTAELDMPLAAAVEMDAATQALLMNSADYAEFHAAFTEKRTPKWQGK"
     aSDomain        complement(8409..9152)
                     /aSDomain="ECH"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_SL103_RS26360_ECH.1"
                     /evalue="1.10E-50"
                     /label="SL103_RS26360_ECH.1"
                     /locus_tag="SL103_RS26360"
                     /protein_end="267"
                     /protein_start="19"
                     /score="164.2"
                     /tool="antismash"
                     /translation="TRQDGVATVTLARPEKLNALTFEAYADLRDLLAELSRERSVRALV
                     LAGEGRGFCSGGDVEEIIGATLGMDTGQLLDFNRMTGQVVRALRECPFPVIAAVHGVAA
                     GAGAVLALAADFRVADPSARFAFLFTRVGLSGGDMGAAYLLPRVIGLGHATRLLMLGDT
                     VRAPEAERLGLISELAEEGRADEAAAALARRLAEGPALAHAQTKALLTAELDMPLAAAV
                     EMDAATQALLMNSADYAEFHAAFTEK"
     misc_feature    complement(9282..9297)
                     /note="TFBS match to CRP, Cyclic AMP receptor protein,
                     confidence: medium, score: 16.38"
                     /tool="antismash"
     gene            9421..9870
                     /locus_tag="SL103_RS26365"
                     /old_locus_tag="SL103_26365"
     CDS             9421..9870
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020114168.1"
                     /locus_tag="SL103_RS26365"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SL103_26365"
                     /product="ATP-binding protein"
                     /protein_id="WP_069571414.1"
                     /transl_table=11
                     /translation="MQDLPAPTTWHIALPHGATAVPVARAMVRTALQDLRATADRTTAQ
                     LLTAELVANALKHAREGAPIELVVVRGPADCRVEVHDGDPVLVEGLGEPPDLSSYGIHV
                     AVPAAAGGRGLLLIRSLSSASGCRATAGGKAVWFTLPEQQRMLQH"
     misc_feature    10340..10351
                     /note="TFBS match to AfsR, Pleiotropic regulatory for
                     antibiotic production, confidence: medium, score: 18.76"
                     /tool="antismash"
     misc_feature    10380..10395
                     /note="TFBS match to ClgR, Caseinolytic protease gene
                     regulator, confidence: weak, score: 22.22"
                     /tool="antismash"
ORIGIN
        1 accgaggccg cggtggtcgg acgggccgac gaggagcgcg ggcaggtcgt cgtcgcgcac
       61 gtcgtgctgc gggacggcgt cccgcgcggg gaggacaccg tcgccgcgct gcgggccttc
      121 accaaggccc gtatcgcgcc ctacaaatgc acgcgcgaca tcgtcttcca cgccgccctg
      181 ccgcgcaccc ccaccggcaa gctccagcgc ttccggctgc gcgcacccga tctagagtga
      241 agcggtgacc gaccagcaga cgcagcgcac cccacggtcc ctcatcgtca cgttctacgg
      301 cgcctacggg cgcggcgggc ccggcaccgc ggggcccgcg accggggcgg tgccggtcgc
      361 cgcgctgatc cggctgctcg gcgtgctggg ggtggacccg ccgtcggtgc gctccgcggt
      421 ctcccggctc aagcggcgcg gcctcctcgt ccccgggcgc accgacgccg gggcggccgc
      481 ctacgggctg tcggagccgg cccgccagct gctggaggac ggcgaccggc ggatcttcgg
      541 ccgaccggcc gcccggctgt ccgacggctg ggtgctggcc gtcttctccg tccccgagga
      601 ggagcggcac aagcgtcacc tgctgcgctc gcgcctggcc cgcctcggct tcggcaccgc
      661 ggcaccgggt gtgtggatcg cccccgccca gctctacgag gagacccggc acaccctgga
      721 gcggctccaa ctgagcccgt acgtcgacct cttcaccggt acgcacgccg gtttcgcccc
      781 gacggcggac gcggtggcgc gctggtggga cctggacgcc gtcgccgcgc agcaccgcgc
      841 cttcctggcg gagcaggagc cggtgctgcg ccgctggtcc cgacggcgca cggtgccgcc
      901 ggaagcggcc taccacgact acctgctggc gctggacgcc tggcgccgtc tcccgtacgc
      961 ggatcccggg ctgccctctc ccctcctgcc ggaggactgg ccgggcggcc gcgccgcgga
     1021 ggtgttcggc cggttgcacg gcacgctcca ggacgcgggg gcccgctacg tgcgcgaggt
     1081 gacgaccggg ccgtgagggc gccgccctca gcggcgtcgc gccggacccc ccatggcgac
     1141 ggccggacgg tgcggcatgc cggccgccgc ccgcggtcgg gcggcggcga tcaccgacac
     1201 cacgtccttg aacgagctgc acatccgggt gccggcgacc gcggcgagct cgccgaacgt
     1261 accgacgccg taggtcaccg ccaccggctc catgcgcgcg gtgaccgcca tcctcatgtc
     1321 ccccaccgtg tccccgacat aggcgctctg ccagggtgcg acgcccaggc ggccggcggc
     1381 tcgcagcacc atctccggat gcggcttgcc gcgccggacc atgtcctggc cgatgaccgg
     1441 cccgaccagg tcgcggatgc ccatgacgtc cagcagcgcc tcggcgctgc gggtggtctt
     1501 ggacgtggcg acggccagct cgacgccgtg ggcgcgcagc gcggagagcc ccgcggtcac
     1561 gccggggaag agcagctgcg ggccctggca caggacttcg taggcgaaca gctcgcggta
     1621 gcgggaaatg gcggcctgca cccgttcgtc gtcctcgggc cggcccagca gccggccgaa
     1681 ggcggggccg gggggcttgc cgatggcggt ggccgcctgc cgggcagtca ccgaactacc
     1741 ctgttcagag gccaccttga ccagtagttt gctgatcgcc gggagggtgt tggcaagcgt
     1801 cccgtccagg tcgaagacgg cggccctgat cgtctcacca ccggcggcac cccgtgcacc
     1861 tctcgcggga cgttcgccga cttccgttgc tacgcgcatg gctggagacc tttccggatt
     1921 ccgatgaggg gtgcggtgtt cggtgacggg tgctggctcg gctggtgggg cggcctgcgg
     1981 tcggtgacag gtgaaggcag aggcggtctt gccatggcgc accgctgtcc tgggacggca
     2041 cgcgaccgtc gcggcggcgg caacggaagg gatcgtcccg tttaccgcct tccgagattt
     2101 aaaaaccgcc tgcgctgttt gtcaatgtgc ctttgtcaca tcctgacggg tcgacaacac
     2161 tcccgtgggg cgccagactg aggaaacacc gtcccgcggg tccccgtaag ggccgccgga
     2221 gcgggcggac atcgggttcg cgtcagggtg tgccgattcc ggccattcac ctgaagaata
     2281 tcgaaccgca tggttggttt tctatgtaga ctgcaatcag ctcgcagccg tactagagac
     2341 gggacggaac cgtgcccacc cagcatcgcg ccatccagag ccgccaggca ctcatccgct
     2401 cggccgcaga gaccttcctg gagaaggggg ttcccgccgc gggcatggtc gagatcagcc
     2461 ggcgggcccg cctgagcaag ggagccctgt acttccactt cacgtccaag gacgacctca
     2521 ccctcgcggt gcgcgacgcc gcgctcgcca ccctgcagga gatcgaggac gccttcaccc
     2581 gttccccgca gccgctgacc accgcggtgc aggacttcgc cgtcgagctg ttcgagcggg
     2641 tggagtccga cgcggtactg cgcgccggac tgcggctgcg gccggagacc gcaccgggcc
     2701 tcgggatcta cgccctggag cagcgctggt acgcgctctt cctcgacaag gcggtgacct
     2761 gcggcaccgg cgggctcggc aggccggcgg cgcacgcgcc ggacgccgaa ccgcgccgca
     2821 ccgcccagct gttgacgtcg atcgtggtgg gtctgctgca cctgggcagc gaggacggca
     2881 catggtggga caaggaggcc gtggtcggcc tctggtccct gctgccggcc gcgccgggac
     2941 ggatcacggc cgccggaatc ccggcaccgg accggctgcc ggcggccggc tgaccgggcc
     3001 cgccgctcgg cgccgtacgt ccgccgccgc gcgtacgccg gtcacaactc caccagtacg
     3061 gcgccgatgt ggtgcccctg ggtgagctcg cacagcgcgc gcggcgcctg ctcgatcccc
     3121 ttcagcaagg cgtgcgggaa gaccagggag ccgtcccgca ggccctggcc gaactccttc
     3181 gtccactcct gcggcagatc cgggtgggcg tgcagcccga agccccgcag caccacccgc
     3241 cgggtgatga tcagccccgc gtccagctcc accggcgcga cggcgccccc gtcaccgccc
     3301 agttgggtcg cgagcgcgcc gaccagcgcg aaccgcgcgt cggggcgggc cgccgcgagc
     3361 gcggcggtga gctgctcgcc gcccacggtg tccagcagta cgtcgatgcc gtccggggcc
     3421 gcccggcgca gctgccggtc gatcggtccg gccccgcgca cgatcgtgtc gtcgtacccc
     3481 agctcggcgc gcagccgggc ggccttgcgc tccgatccgg tgctgcccac gacccggccg
     3541 gcccccagcc gccgggcgag ctgcccggcc aggctgccca ccccgcccgc cgcgccggtg
     3601 acgaacacgg tgtcaccggg gcggacctcc accgcgcggg tcagcgcacc ccaggcggtc
     3661 gccccctgtg acaggtgcgc ggcgggggtg ggcagcgcgt ccggggccag ccggcgcagc
     3721 cgcccctcgt cgaccaccgc gaactcccgc cagccgtagg ggtggtggac gagctcaccg
     3781 ggcgcgaagc cgccgccggg ggccgccacg acctcaccga ccgcggagcc ccacagcgcc
     3841 tggccgggca cgaaggaggg catcggcagc ggggcgccgg tcatctgggt ccgcatcgcc
     3901 gcggtcaccg ccatcacccg cgtccgcacc aggacctgac cggggcccgg ctgcggcacc
     3961 ggcacctcgg cgagcgtgaa gtgccgcgtg gtcagctgcc cttcggcgcg ggcggcgagc
     4021 cgcacctccc gctgaacggc gggtacgtcg ggaacggcgg tgggggtcat cgggggcggc
     4081 tcctcacatt cgtggccgcg gacggcgcgg cgcggcacgc tgcagcttca ataccgcatg
     4141 gttggcttct ttatgatgcg caaatagact gcgcggccac ggcggcgggt cgcactcggg
     4201 cgcaacaact ccaagagagc atacggagga tgatcggtgg tcaagcagga acgcgcagtg
     4261 cgcacccggc gcgccgtcct cgaagccgcg gctcatgtca tcggcacccg cggctaccag
     4321 gccgccacga tggccgagat catccagcgc gccggcgtca ccaagggagc ggtgtacttt
     4381 cacttcacgt cgaaggacgc actggcccgc gcggtgatca ccgagcagac cgatcccttc
     4441 ctgccgcagg tcagcgagtc ccggctgcag gacgccatcg acttcaccca ccaggtggcg
     4501 ctggcgctgc gcagcgaccc gttgttgcag gccggcaccc gtatcgcggt cgagacgacc
     4561 ttcagcgacg agcccctggt gccgtaccag gcgtggaccg acatcatcac cacgatgttc
     4621 agcgaggccc gggacaacgg ggaattactg ccgggggtgg cgcccgaccg ggcggccgag
     4681 ttcttcgtcg ccgcttatat gggcgtgcag ttgttctcgc gggcggcgac caaccgcgcg
     4741 gatcttcccg agcgggtcac cgcgctgtgg aaacacaccc tgccgggcct tgcctcgccc
     4801 ggcgcgatga gtcatctcga tccgcacggc cgcgccgtgc aggtctccgt ctgacggcag
     4861 cgcccccttt cggggcggag gaaccggttc attggcacta gaaaacaaac cacatggtcc
     4921 gtatcttggt gcggcagggc cgcggcagtg agtctcagac cctcaggacg ctgagcgccg
     4981 cacttcagaa ggggatacct atggccagcg ccaccctcat gccgtcctgg cgcgatcagc
     5041 gccatgacga cagaggccgg ttcgccgacg gaaaccgacc gtccgctttg accacgaccg
     5101 tgccgcggga gtacgtccac cgcaccgccg tgtccgaggt gttcctgacg aactggcggc
     5161 gcggcaccgc tgacagctgg gtcgtgagcg cccagtggcc gcgcgcccac agcttctacg
     5221 gcccggtggg cggcctgcac gatccgctgt tactgatcga gacgatgcgg caggccggga
     5281 tattactgag ccatgtcgcg caccgcgtgc cgctcgacca ccccatcatc tggcagcggg
     5341 tccgctacga cctcaccccg caggccctgc gcgccgccga cgaaccggcc gaggtggagc
     5401 tgcacatcac cgaccatgac gtgctccgcc gcggcaagcg gctgatcagc gcccgtcagc
     5461 acttccgcat ccggtgtgac ggcgcggacc tggccaccgc gtcgctcgac tactcctgcc
     5521 acagtcccgc cgtctaccgc cggctgcgcg gcaagtacag cgacctgacg ctggccaacg
     5581 cccagcggct gccgctgccc gagccggtga ccccgcggct ggtgggccgg gaccgggagc
     5641 gcgacgtggt gctctccccc acgaaccggc ccgaccgctg gcagctgcgg gtggacacct
     5701 cccacccggt gctcttcgac catcccgtcg accacgcccc cggcatgctg atgatcgagg
     5761 ccgtccggca ggccgcgcag gccgcggcgc cggggctgac cctgccgacg gcgatggagt
     5821 gcgacttcga gcggtacgcc gaactggacg ccccctgctg ggtgcaggcg cgcgccgcgg
     5881 acgggccccg gccggcggcc gctgacggcg gccggcagat cgaggtgtcc gtggaacagc
     5941 acggcaaacc ggtgacggcg gcgcggatca ccagcgtgcc cacctcctga caccgccctg
     6001 aggagccgcc gccggatcgg cgccccgaaa cggccccgcc ccgctgcccg tgcctccccg
     6061 tcaccgcagc gtaaggcggg gcttcggcgc gtcggtacgc ccggtgggcg gggtgcggct
     6121 gcccgcctgg tagggcagcg gccagggggc gccctgtccc gcgtacccct gttccgcggc
     6181 ggcgtgcagc gtccagtgcg ggtcgtacag gtgcggcctg gccagcgcgc acaggtccgc
     6241 ccggccggcc aggaccaggg agttgacgtc gtcccaggag gagatcgcgc cgaccgcgat
     6301 gaccggtacg ccgaggctgt tgcggatccg gtcggcgaac ggcgtctggt acgaccgtcc
     6361 gaagtcggga cgctcgtcgg cgacgacctg cccggtggag acgtcgatgg cgtcggcgcc
     6421 gtgctcggcg aaggccgccg cgacggtgac cgcgtcctcg gcggtggtgc cgccgtcggc
     6481 ccagtcggtg gcggagatgc ggaccgtcat cggcttgtcg gccggccaca gcgcacggac
     6541 ggtgtcgaag acctccaggg ggaagcggag ccggcgggag agggaacctc cgtaggcgtc
     6601 ggtgcgttga ttggtcagcg gggagaggaa tccggacagc agatagccgt gggcgcagtg
     6661 cagttcgagc aggtcgaagc cgcagtcgac ggcgcggcgc gcggcggcgg cgaactgctc
     6721 ccggatgccg tcgagttcgt ccggcgtcag ctcctggggg cgctggctgc cgggcttgta
     6781 gggaaggggg gagggggcga cgaggggcca gttgccgtcg tcgagcggtt cgtcgatgcc
     6841 gtcccacatc agcttcgtgg agcccttgcg gccggcgtgg ccgagctgga gtccgatcgc
     6901 ggcaccgggc gcctgcgcgt gcacgaagtc cgtgacccgg cgccaggccg cggcctgctc
     6961 aggggtgtag agcccggcgc agcccggggt gatgcggccc cgttcgctga cgcagaccat
     7021 ctcggtcatg accagccccg cgccgcccag ggcccgggcg ccgaggtgga cgaggtggaa
     7081 gtcgccgggg acgccgtcct gcgccgagta catgtccatg ggcgagacca cgacgcggtt
     7141 gcgcagggtc aggccgcgca gccggaacgg cgtgaacatc ggcggggtcc cggccgggac
     7201 ggccgcctcg tcctcgaccg ccgcgacgaa tccggggtcg cgcagccgca ggttgtcgtg
     7261 ggtgacgcgg cggctgcggg tgaggaggtt gaaggcgaac tggcggggcg gctggtcgac
     7321 gtaggtggcc agctcctcga accacgcgag gctggcgcgg gcggcccgct gggtggaggc
     7381 gacgacgggg cggcgctccg cctcgtacgc ggccagtgcg gcggcggtgt cgggctgctc
     7441 ctggaggcag gcggccagcg ccagggcgtc ctcgacggcg agcttggtgc cggagccgat
     7501 ggagaagtgc gcggtgtgcg cggcatcgcc gagcagcacc gtgttgccgt gcgaccagcg
     7561 ctcgttgacg accgtgcgga aggcgatcca gctggagttg ttggagcgca gcggccggcc
     7621 gctcagggca tcggcgaaga ccttggcgca ccactccgcg gattcgcgct cgtcggcgcg
     7681 gtcgagcccg gcggcccgcc acacctcctc gcgcatctcg acgatgacgg tgctggcgcc
     7741 gcccggatgc cgcggcccgg cgggcgccgc cccggggcgc gagtcgggtg ccgggcggga
     7801 gtaggggtag gcgtgcagct ggacgacgcc gtgcgcggtc tcggcgatct cgaagcggaa
     7861 ggcgtcgagg gcgaagtcgg cggcgagcca gatgtagcgg cagcggtggg tggtcagccg
     7921 ggggccgaag gcgtcggcgt gtgcggtgcg ggtggcgctg tgcaccccgt ccgcggcgat
     7981 caccaggtcg tgggtgcggg ccagttcggc ggcgggcggc gcctcggtcc ggaagcgcag
     8041 ctcgatgccg aggtcgcggc agcgccggtg caggatgccc agcagccggc gccggccgag
     8101 cgccgcgaag ccgtgtccgc cggaggtcag ggtgtggccc cggtgcacga tgtcgatgtc
     8161 gtcccagcgg acgaactccc gctggagcgc gcggtagacc tccgggtcgg cgtgctcgat
     8221 gccgccgagg gtctcgtcgg agagcaccac gccgaagccg aaggtgtcgt cgggcgcgtt
     8281 gcgctcccaa acggtgatct cgcgggtggg gtcgaggcgc ttgaggagga cggcggcgta
     8341 gagcccgccg gggcctccgc cgatgacggc gacccgcatg gctatttccc ctgccacttg
     8401 ggcgtccgct tctcggtgaa ggcggcgtgg aactccgcgt agtcggcgct gttcatcagc
     8461 agcgcctggg tggcggcgtc catctcgacg gcggcggcga gcggcatgtc gagttcggcg
     8521 gtgagcagcg ccttggtctg ggcgtgcgcg agcgcgggcc cctcggcgag ccgccgggcg
     8581 agggcggcgg ccgcctcgtc ggcccggccc tcctcggcca actcgctgat cagtcccagc
     8641 cgttcggcct ccggggcgcg gacggtgtcg ccgagcatca gcagccgggt ggcgtggccg
     8701 agaccgatca cgcggggcag caggtaggcc gcgcccatgt caccgccgga cagtccgacg
     8761 cgggtgaaga ggaaggcgaa gcgggcggag gggtcggcga cgcggaagtc cgcggccagg
     8821 gccagtaccg cgccggcgcc ggccgcgacg ccgtgcaccg ccgcgatcac cgggaacggg
     8881 cactcccgca gcgcccgtac gacctggccg gtcatccggt tgaagtccag cagctggccg
     8941 gtgtccatac caagggtggc gccgatgatc tcctcgacat cgccgccgga gcagaatccg
     9001 cgcccctccc ccgcgagcac cagggcgcgt accgagcgtt ccctggacag ctcggcgagc
     9061 agatcgcgga ggtcggcgta cgcctcgaag gtgagcgcgt tgagcttctc ggggcgtgcc
     9121 agggtgacgg tggcgacccc gtcctgcctg gtcacgcgga tgtgctgcca ggggtctgtg
     9181 cggtgggcgg atcctgcgaa cgggctcatc gggctgcctg ccccctttgg cgggtcggcc
     9241 ggtggatgtt ctccggatgc ccttcgaagt tatcaccgct tcgtgactgc cgtcacgagg
     9301 gcgcgatacg cgcgccggac ggctgggccg aaggtcccgt cacccgtaag acgtatggca
     9361 ccccgaagcc ggccgctcgg ctctgcagcc gggccgcccg cgctccctac cgtgaagggc
     9421 atgcaggacc tgccggcccc cacgacctgg cacatcgcgc tgccgcacgg cgccaccgcg
     9481 gtgccagtgg cgcgcgccat ggtcagaacc gcgctgcagg acctgcgggc caccgccgac
     9541 cggaccaccg ctcagctgct gaccgccgag ctggtcgcca acgcgctcaa gcacgcccgc
     9601 gagggcgccc cgatcgagct ggtggtggtg cgcgggccgg cggactgccg ggtggaggtg
     9661 cacgacggcg atccggtgct cgtcgagggg ctcggcgaac cgcccgacct gtcctcttac
     9721 gggatccacg tcgccgtgcc ggctgcggcc ggcggccgcg gtctgctgct gatccgctcc
     9781 ctcagctcgg cgtccggctg ccgggcgacg gcgggcggca aggcggtctg gttcaccctt
     9841 cccgagcagc agcggatgct ccagcactga cggcggcccg ggccggcccg gccggcgact
     9901 gccgctcctg gcgcgccgcc tgctgcccga gccgagagtg gcgccgcccg tagagcgcgt
     9961 agaccagcgc accggccagc aggaagccgg cgaactgcag ccaggtcgcg aggccggtcc
    10021 cgtagatcag gtaggcgcag aagccgatgc cgaggaccgg gccggccggg aagagcggga
    10081 cgcggaagcg gcgcggcagg tcggggcggg tgcgccggag caccagcacg ctgaggttga
    10141 ccaccgccat gaccgccagc gtcccgatgg tggtcaggtt catcaccacg tccaacggaa
    10201 cgaccgcggc cggcacggcg aacaccgccg cgacgatcca ggtgttggcg accggggtgg
    10261 acgtccgggg cgagacccgc tcgaagatcc gcgggaccag cccgtcccgc gccatcgaca
    10321 tcaggatgcg ggtctggccg tacatgaccg cgagcacgac ggaggcgatg gcgacgacgg
    10381 cgccgaaggc gatcaccccg ccgccgagcg tcgagtgggt gacgcggttg acgatcagcg
    10441 acagcgcggc gggccggtcg gcgacctggt cggcggagag cgcaccgacg gcggcgagtg
    10501 cgaccaggca gtacaggacg gtgaccacac cgatgcagac catgatcgcg acggggatgt
    10561 tgcgccgggg gttgcgcacc tcttcgccgg ccgtggtgat cgcgtcgaag ccgatgtacg
    10621 agaagaacgc gaccgacgcg cccgaggtga tcccggcgat gctctgcggt gcgagcggcg
    10681 agaggtggcc ggcccggaag gcgctgaagc cgatcacgca gaacagcacc aggaccgcga
    10741 gcttgaggac cgccatcgcg gcggtggcac gggcgctctc gcggatgccg cgcaccagca
    10801 gcagggcggc gagcagcatc acgaccacgg ccggcacatt gacgacgccg cccgcccccg
    10861 gcggattgga gagcgcggcc ggcagctgcc ggccgatcac gctgccgagc agttcgttga
    10921 ggtactggcc ccagccgacg gcgaccgccg agaccgagat gccgtattcg agcagcaggc
    10981 accagccgac
//
