LOCUS       NZ_CP017157            11222 bp    DNA     linear   CON 31-MAY-2024
DEFINITION  Streptomyces lydicus strain 103 chromosome, complete genome.
ACCESSION   NZ_CP017157
VERSION     NZ_CP017157.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces lydicus
  ORGANISM  Streptomyces lydicus
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 8201357)
  AUTHORS   Jia,N., Ding,M.-Z., Gao,F. and Yuan,Y.-J.
  TITLE     Complete genome sequencing of Streptomyces lydicus 103 and metabolic
            pathways analysis of antibiotic biosynthesis
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8201357)
  AUTHORS   Jia,N., Ding,M.-Z., Gao,F. and Yuan,Y.-J.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2016) Key Laboratory of Systems Bioengineering
            (Ministry of Education), Tianjin University, Tianjin 300072, PR
            China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SMRT Analysis v. 2.3.0
            Genome Coverage                   :: 100x
            Sequencing Technology             :: PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_001729485.1-RS_2024_05_31
            Annotation Date                   :: 05/31/2024 02:23:15
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,288
            CDSs (total)                      :: 7,195
            Genes (coding)                    :: 7,061
            CDSs (with protein)               :: 7,061
            Genes (RNA)                       :: 93
            rRNAs                             :: 7, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 47 of 134
            Pseudo Genes (incomplete)         :: 104 of 134
            Pseudo Genes (internal stop)      :: 11 of 134
            Pseudo Genes (multiple problems)  :: 26 of 134
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 01:52:31
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 5993230
            Orig. end                         :: >6004452
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP017157.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Source DNA is available from Prof. Ying-Jin Yuan.
            Annotation modified by submitter.
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..>11222
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="25"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..>11222
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..>11222
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:>6222](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..>6222
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            complement(485..895)
                     /locus_tag="SL103_RS26070"
                     /old_locus_tag="SL103_26070"
     CDS             complement(485..895)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="SL103_RS26070"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="SL103_26070"
                     /product="hypothetical protein"
                     /protein_id="WP_069571364.1"
                     /transl_table=11
                     /translation="MSGTHARPAPAPAPRPYPGPTAPTAPTECYADHDATSTATPVTAT
                     PGNETPGTVTPGNETPGTAATSNAALSNETTSNAAPGTAALSNATTSTDAASPPADCLQ
                     CGEPTEYPVEQPGIVLCPVCEWQEAQRSACSG"
     gene            complement(1288..1956)
                     /locus_tag="SL103_RS38405"
                     /old_locus_tag="SL103_26075"
     CDS             complement(1288..1956)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006602441.1"
                     /locus_tag="SL103_RS38405"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SL103_26075"
                     /product="ABC transporter"
                     /protein_id="WP_069571365.1"
                     /transl_table=11
                     /translation="MIALLRYQAALFIGSHRWLPPVIFHAAFLAVGIQSGGPILDAFGW
                     AAGGLLPVAVWLTRVCVTNEPPAARSCTAAAAGPGRVHLASVLTAAGAGVLLALLGALV
                     VLLLSDPHSGDHRVAVPVLPATVAGLLAALACLLLGTAIGTLCNWPVVRNPGWGIPTGL
                     TAALLLLVLGASPANAAVTGLVTGSAHGTVNTPWLPLAATVAIAAATTAAACALTSRRT
                     "
     gene            complement(1953..2954)
                     /locus_tag="SL103_RS38410"
                     /old_locus_tag="SL103_26080"
     CDS             complement(1953..2954)
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018536943.1"
                     /locus_tag="SL103_RS38410"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SL103_26080"
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="WP_079146300.1"
                     /transl_table=11
                     /translation="MRLDGVGRRYGVRGPWVLRDVHLEVPRGALLRVEGTNGSGKSTLL
                     RLLAGIDHPSTGRITGRPRSAYVPERFPVALPFTAVGYLTHLGRVHGLRGAEAARRAAE
                     WLERFGAAGHARTRLAELSKGTSQKVAVAQALLAEPTLLVLDEAWTGLDRGAREVLDRA
                     VAERVADGGTVVFVDHDPQRLAGAADASYRVAEGRLLPHEAAGPAAGSETATGPRVIIE
                     AEGAPGAVPPVFAGAPGLPGGPEITPGPDGTTRLTVAAVHSDALLRTLLAAHPPWHIRS
                     LTPAAPRTPTPPSPAPTSGSRPDAHSEAHPDSTSTSAFTSTSASDSDEAPLP"
     gene            3289..4053
                     /locus_tag="SL103_RS26085"
                     /old_locus_tag="SL103_26085"
     CDS             3289..4053
                     /EC_number="3.1.1.29"
                     /GO_function="GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006602443.1"
                     /locus_tag="SL103_RS26085"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SL103_26085"
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="WP_069571366.1"
                     /transl_table=11
                     /translation="MTSTETAGEPGNSTGSPFRHEETDRDAAPQFVLPLVARIERAEPP
                     ARTDALETAARAVLVLLADERSAGDGEWAQAVRDWQDARIRKVVRRARGAEWRRAEALP
                     GITVGGRDAEVRVFPPVPLDGWPKELARLQVSGTELDDPLPPEAPADGLPVLWLNPELE
                     MSAGKEMAQVGHGAQLAWWELSEEERAAWRGTGFALAVRSAEPAEWAALTRSGLPVVRD
                     AGFTEIAPGSCTVVADHPALRGALPYEGPQGG"
     gene            4195..4875
                     /locus_tag="SL103_RS26090"
                     /old_locus_tag="SL103_26090"
     CDS             4195..4875
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1116:
                     Homoserine/threonine efflux protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016578488.1"
                     /locus_tag="SL103_RS26090"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SL103_26090"
                     /product="LysE family translocator"
                     /protein_id="WP_069571367.1"
                     /transl_table=11
                     /translation="MLTSLVGFTAVAALLTVSPGPDFAVVLRTALSSGRRAALCSALGI
                     AAGCFVWGLAGAVGLTAMLSASQAAYHALRVAGALYLMWLGVQALRSARRLRPARADSA
                     APAHVRAPHESTVAPDSSAGSVTPLRAFRTGLLTNVLNPKVGVVYISLLPQFIPHGAPV
                     VATTVLLVAVHAALGVLWLGGIAVAVHRARAVFQRPGVRRRLDQATGGVLLALGAAVGF
                     ETAR"
     gene            5001..>6222
                     /locus_tag="SL103_RS38415"
                     /old_locus_tag="SL103_26095"
                     /pseudo=""
     CDS             5001..>6222
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: DUF692"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014675658.1"
                     /locus_tag="SL103_RS38415"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /old_locus_tag="SL103_26095"
                     /product="DUF692 domain-containing protein"
                     /pseudo=""
                     /sec_met_domain="DUF692 (E-value: 1.3e-97, bitscore: 324.9,
                     seeds: 7, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MERLGTGIGWRPEIAADIAELPGVDWVEVVAENLCPGHLPPALQQ
                     LRERGTTVVPHGVSLGLGGADRPDPGRLTALAERAEALGAPLVTEHIAFVRAGGPLTAS
                     PVMEAGHLLPVPRTRDALDVLCENVRIAQDALPVPLALENIAALVNWPGEEMTEGQFLS
                     ELVERTGVRLLIDVANLHTNHVNRGEDPAVALDELPVEAIAYVHVAGGVERDGVWHDSH
                     AHPVTQPVLDVLAALCARVTPPGVLLERDDDFPEGPELAGELDAIRTVLREARHAYAA"
     gene            6465..7469
                     /locus_tag="SL103_RS26100"
                     /old_locus_tag="SL103_26100"
     CDS             6465..7469
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018100704.1"
                     /locus_tag="SL103_RS26100"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SL103_26100"
                     /product="TIGR04222 domain-containing membrane protein"
                     /protein_id="WP_069571368.1"
                     /transl_table=11
                     /translation="MSFAEWVYLGLGVSSLVLVIGTVVLRLRTPSVRRADALAHDVWET
                     AFLAGGPARVVDAAVAGMHEDGRLAVGGPGVVTVRQATARDAVEAAVLDAVARTPGGAL
                     AALRAEAMRSPAVQDVGDRLAARGLMRHPRLGRGWLLWARAHQAACGVLFVVGMVLSVP
                     GDDGNDGQTVVMILPALIGGLVVGSLCRKAFRRRITRAGSAALRSARAAHPQGDVGGAW
                     QAAGLVVALGGTALLADELLRQQFEEAHRASALAASGAGSSSSGGSSSGFDSGGGSGDG
                     GSWCGSSDGGSGSSCGGSSCGAGSSCGGGSSCGGGSSCGGGSSCGGGSGCGGG"
     misc_feature    complement(7600..7614)
                     /note="TFBS match to SypG, Regulator of biofilm formation
                     and host colonization, confidence: weak, score: 16.24"
                     /tool="antismash"
     gene            7656..8474
                     /locus_tag="SL103_RS26105"
                     /old_locus_tag="SL103_26105"
     CDS             7656..8474
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018088889.1"
                     /locus_tag="SL103_RS26105"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SL103_26105"
                     /product="TIGR04222 domain-containing membrane protein"
                     /protein_id="WP_069571369.1"
                     /transl_table=11
                     /translation="MWLLFLLVACVAAALSCAQLCRAAVAVARATGPAPRPAPAPVPDT
                     AAPDGSAAGLTLYEMAYLSGGPHRLADVVLVLMAQQRRLLLAHTGWATVVDPVGQDPVE
                     RATLSAIGPDGQRRIPAIREALVADEAVRAVGDRLVAAGLALPAAARGLRNAVRQVQGA
                     TVLALLCAAAAYATAPAGEDRGQLLIWFTLPLILTLGTLAVARFELHPYSDWATDAGEE
                     RLPGRPGFTTFTPSSPAPVAPLASLMTLALHGPRALTDPALRAALHSSGA"
     misc_feature    8530..8543
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 12.88"
                     /tool="antismash"
     misc_feature    complement(8531..8544)
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 12.32"
                     /tool="antismash"
     misc_feature    complement(8532..8545)
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 13.17"
                     /tool="antismash"
     gene            8560..10245
                     /locus_tag="SL103_RS26110"
                     /old_locus_tag="SL103_26110"
     CDS             8560..10245
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /NRPS_PKS="Domain: Abhydrolase_1 (93-507). E-value:
                     1.3e-16. Score: 52.9. Matches aSDomain:
                     nrpspksdomains_SL103_RS26110_Abhydrolase_1.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF00561"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006602445.1"
                     /locus_tag="SL103_RS26110"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SL103_26110"
                     /product="alpha/beta hydrolase"
                     /protein_id="WP_244304047.1"
                     /sec_met_domain="PF00561 (E-value: 2.5e-23, bitscore: 81.7,
                     seeds: 48, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRALALYGTIGSLIVTALATAPAGGATEAPDAPPAPVAHRIGFGR
                     CAPVEQLPASVECGKLAVPLDYARPDGKKIELTVSRIRATVPGERQGSLVFNPGGPGAS
                     SLEFPLYGGLPKWSKVARAYDFVGYAPRGVGRSAPLSCQDPKEFAKVPTDSRMHPDAAF
                     KRAKVAQARAYARGCARRAGADLPFYNSVNNARDLDMLRAALGEKKLTYMGASYGTYFG
                     AVYATLFPGHVRRMIFDSVVNPEPRQIWYRNNLDQNIAFERRWGDWLRWVAKHDDHYHL
                     GATRQAVQRAYDQAAAKLRRDPLDGKIGPGQLQSAFLKTGYNDAFWPMRAEALSGYLHG
                     DPKALIAQALPQSSGARDEENANAVYTAVECNDGPWPRSWQTWDRDNTEVARIAPFETW
                     DNAWMNLPCAFWPDRSESAEAGVEQAARRAEHGNGQELADATGHAARTAMDGALHGPRG
                     PLDVHTEPGALPPVLLLAAERDAATPYKGAVELQHRLPGSSLVTENGAGTHGVAFNDNA
                     CATRYAEAYLLQGKIPSHRVYCAPRAEPVPGQKAVRAQLARPGK"
     aSDomain        8839..10080
                     /aSDomain="Abhydrolase_1"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_SL103_RS26110_Abhydrolase_1.1"
                     /evalue="1.30E-16"
                     /label="SL103_RS26110_Abhydrolase_1.1"
                     /locus_tag="SL103_RS26110"
                     /protein_end="507"
                     /protein_start="93"
                     /score="52.9"
                     /tool="antismash"
                     /translation="LVFNPGGPGASSLEFPLYGGLPKWSKVARAYDFVGYAPRGVGRSA
                     PLSCQDPKEFAKVPTDSRMHPDAAFKRAKVAQARAYARGCARRAGADLPFYNSVNNARD
                     LDMLRAALGEKKLTYMGASYGTYFGAVYATLFPGHVRRMIFDSVVNPEPRQIWYRNNLD
                     QNIAFERRWGDWLRWVAKHDDHYHLGATRQAVQRAYDQAAAKLRRDPLDGKIGPGQLQS
                     AFLKTGYNDAFWPMRAEALSGYLHGDPKALIAQALPQSSGARDEENANAVYTAVECNDG
                     PWPRSWQTWDRDNTEVARIAPFETWDNAWMNLPCAFWPDRSESAEAGVEQAARRAEHGN
                     GQELADATGHAARTAMDGALHGPRGPLDVHTEPGALPPVLLLAAERDAATPYKGAVELQ
                     HRLPGSSLVTENGAG"
     gene            complement(10335..11087)
                     /gene="hemQ"
                     /locus_tag="SL103_RS26115"
                     /old_locus_tag="SL103_26115"
     CDS             complement(10335..11087)
                     /EC_number="1.3.98.5"
                     /codon_start=1
                     /gene="hemQ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003984746.1"
                     /locus_tag="SL103_RS26115"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SL103_26115"
                     /product="hydrogen peroxide-dependent heme synthase"
                     /protein_id="WP_069574119.1"
                     /transl_table=11
                     /translation="MTDASTPASAPQAPAAPEKAPNAGKKAKDLNEVIRYTLWSVFKLR
                     DVLPEDRSGYADEVEELFAQLAAKDVVVRGTYDVSGLRADADLMIWWHSESSDALQEAY
                     NLFRRTRLGRALAPVWSNMALHRPAEFNKSHIPAFLADEQPRNYVSVYPFVRSYEWYLL
                     PDEDRRRMLADHGKMARGFPDVRANTVPSFSLGDYEWVLAFEADELYRIVDLMRHLRGS
                     EARMHVREEIPFYTGRRKPVAELVAGLA"
ORIGIN
        1 cgcgcaccgc gccgccggct cggcggccga ccactacggc caggtctgga agtacgaccc
       61 cgaacggcgc cggctcaccc aagtcgtggt cttcggcccg gacagcgacc tccaactgcc
      121 cggcgagtca cccgacaaca tctgcctcgc ccccagcggc ggcctgatgg tctgcgagga
      181 cggcgacggc gcccagcacg tcttcggcct cacccgccgc ggcgaggtct acgcgatggc
      241 gcgcggccgg cagaacatcg gcaccccgga gaagccggaa tggggcgagt tcgcgggcgt
      301 gacgttcgcc cccgaccacc gcaccatgta cgtcaactgc tacaccccgg gcacgacgtt
      361 cgccgtgacg ggtccttggg ggcactgagg cgggtgccct gacgcggacc ccggggcggg
      421 ggcccggcgc cgggcggatg gaactgcctg cgtacggccg ggcgcggtgc ccggcaccgc
      481 cgcgtcagcc ggagcaggcg gagcgctggg cctcctgcca ctcgcacacg gggcagagca
      541 cgatgccggg ctgctcgacc gggtactcgg tgggctcccc gcactgaagg cagtcggccg
      601 gcggggaggc ggcgtcggtg ctggtcgtcg cgttgctgag cgctgcggtg ccgggcgctg
      661 cgttgctggt cgtctcgttg ctgagcgctg cgttgctggt cgctgcggtg cccggcgtct
      721 cgttgccggg cgtcacggtg ccgggcgtct cgttgccggg cgttgcggtg accggcgtcg
      781 cggtgctcgt cgcgtcgtgg tccgcgtagc actcggtggg ggccgtgggt gccgtggggc
      841 ccgggtacgg gcggggcgcg ggggcggggg cggggcgggc gtgggtgccg gacatggtgt
      901 cgagcgtacg ccggtgcctc cccccccacc cccgaaccca ccccccgccc cctcccccga
      961 agggggaggg ggggaggggg cggggggagc gccgatgagc cggcgacggc ggcggatggg
     1021 cgaagcgtcc atgagccggc gacggcggcg gatcggcgaa gcatccatga gcccgcgagg
     1081 gcggcggatc ggcgaagcat ccatgagccc gcgagggcgg cggatcggcg aagcatccat
     1141 gagcccgcga gggcggcgga tcggcgaagc gtccatgaca cgggaaaggc ggacggggca
     1201 agtgctgggt gagcggtgcg taggccggga aagccgcgat ctacgcagga caccggggcg
     1261 ggcacgtgcg cgggcggggg ccggcgctca agtgcggcgg gaggtgaggg cgcaggcggc
     1321 tgccgtggtg gcggcggcga tcgcgacggt cgcggcgagc gggagccagg gggtgttgac
     1381 ggtgccgtgg gcggagccgg tgaccaggcc ggtgacggcg gcgttggcgg gcgacgcacc
     1441 gaggacgagc agcagcagcg cggcggtcag tcccgtgggg atgccccagc ccgggttgcg
     1501 tacgaccggc cagttgcaca gcgtgccgat cgcggtgccc agcagcaggc aggccagcgc
     1561 ggcgagcagc cccgcgacgg tcgcgggcag gaccgggacg gcgacgcggt ggtcaccgga
     1621 gtgcgggtcg gacagcagca gcaccaccag cgctcccagg agcgcgagca gcaccccggc
     1681 accggccgcg gtcagcacgg acgcgaggtg cacccggccc ggcccggcgg cggcagcggt
     1741 gcaactgcgg gcggccgggg gctcgttggt gacgcagacg cgggtgagcc atacggcgac
     1801 cggcagcaag cctcccgcgg cccagccgaa cgcgtccagg atcgggccac cgctctggat
     1861 accgacggcg aggaacgccg cgtggaagat gaccggcggc agccagcggt gcgagccgat
     1921 gaacagcgct gcctggtagc ggagaagagc gatcacggaa gcggcgcctc gtcggagtcg
     1981 gaagcggaag tggaagtaaa agcggaagtg gaagtggaat cggggtgggc ctccgagtgg
     2041 gcgtccgggc gtgatcccga ggtgggcgcg gggctcggag gggtcggggt ccggggtgcg
     2101 gcaggggtca gcgagcggat gtgccagggc gggtgggccg cgagcagggt gcgcagcagc
     2161 gcgtcggagt ggacggccgc gacggtcagc cgggtcgtac cgtccggccc cggggtgatc
     2221 tccgggccac cgggcagccc cggcgctccg gcgaagacgg gcggtacggc gcccggggcg
     2281 ccctcggcct cgatgatcac gcgggggccg gtcgcggtct ccgatccggc tgccggcccg
     2341 gccgcctcgt ggggcagcag ccgcccctcc gccacccggt agctggcgtc ggccgcgccg
     2401 gccagccgct gcggatcgtg gtcgacgaag acgacggtgc cgccgtccgc gacccgttcg
     2461 gcgaccgcgc ggtccagtac ctcgcgcgcg ccgcggtcca gcccggtcca tgcctcgtcc
     2521 agcaccagga gcgtgggctc ggcgagcagc gcctgcgcca cggcgacctt ctggctggtg
     2581 cccttcgaca gctcggccag ccgcgtacgg gcgtgtccgg ccgcgccgaa gcgttcgagc
     2641 cactcggcgg cgcggcgggc ggcttcggcg ccgcgcaggc cgtggacgcg accgaggtgg
     2701 gtgaggtagc cgaccgcggt gaacggcagc gccaccggga agcgttcggg gacgtacgcg
     2761 ctgcgcgggc gtccggtgat gcggccggtg ctggggtggt cgatgccggc gagcagccgc
     2821 agcagggtgg actttccgct gccgttggtc ccctccaccc gcagcagcgc ccctcggggc
     2881 acctccaggt gtacgtcccg cagcacccag gggccgcgca ccccgtagcg ccgccccacc
     2941 ccgtccagcc tcatggccgg cgccacatcc ggcactccgg cacctcccca gcccgtgtcc
     3001 tgacgccccc cggcgtctcc tggcgtcccg gcgccgacgg tagccggggc ggcgggacgg
     3061 cgcccgcccg ggcacgcgtg gtgcgacatc cccgcgcggg cgcgcagggg gaacgcgtac
     3121 ggcacggcca acgcgccaac gccacgacgg aggtgggaag ttggccgccg gccggttggg
     3181 tcccggccgg gtggccgtgg cttgccgggc ggaagccgtc ggccggtggc ctcgggcggg
     3241 cgccggtccc ggcccggcgt tgtcggtggc ggctggcaga ctgggcgagt gaccagcacc
     3301 gagaccgccg gagagcccgg caacagcacc ggcagcccgt tccggcacga agagaccgac
     3361 cgggacgcgg cgccccagtt cgtgctgccg ctcgtcgcgc ggatcgagcg ggccgagccg
     3421 ccggcgcgta cggatgcgct ggagacggcg gcgcgggcgg tgctggtgct gctggccgac
     3481 gagcggtcgg ccggcgacgg tgagtgggcc caggcggtac gggactggca ggacgcccgg
     3541 atccggaagg tggtgcggcg ggcgcgcggc gcggagtggc ggcgggccga ggcgctcccg
     3601 ggaatcacgg tcggcggccg cgacgccgag gtgcgggtct tccccccggt gccgctcgac
     3661 ggctggccga aggaactggc gcgcctccag gtctccggca cggagctgga cgatccgctt
     3721 ccgcccgagg cgcccgccga cgggcttccg gtgctgtggc tcaatccgga gctggagatg
     3781 tcggcgggca aggagatggc gcaggtcggc cacggcgcgc agctggcgtg gtgggagctg
     3841 tccgaggagg aacgggcggc ctggcgcggg acgggtttcg cgctggcggt ccgttcggcg
     3901 gagcctgcgg agtgggcggc gctgacccgc agcgggctgc ccgtggtgcg ggacgccggg
     3961 ttcacggaga tcgcaccggg ctcgtgcacg gtcgtcgcgg accaccccgc actgcgcggg
     4021 gcactgccgt acgagggccc gcagggcggc tgaacgacat ccggcccgga gtcacgcccg
     4081 gcccggcccg cccactcccc cgtccgggag cgacgcccgg ctcttcctgg cccccgtgct
     4141 ttccgcagtg cccgggctct ccgcagtgcc cgccactgcg cataccgttg cgccatgctc
     4201 acctcactcg tcggcttcac cgccgtggcc gccctgctga ccgtctcccc cggacccgac
     4261 ttcgccgtgg tgctgcggac cgcgctcagc tcggggcggc gcgccgcgct gtgcagtgcg
     4321 ctcggcatag ccgccgggtg cttcgtctgg gggctggccg gtgcggtcgg gctgacggcg
     4381 atgctctcgg cgtcgcaggc ggcgtaccac gccctgcggg tggccggtgc gctgtatctg
     4441 atgtggctcg gggtacaggc gttgcgcagt gcccggcggc ttcgccccgc gcgcgccgac
     4501 agcgccgccc cggcccacgt tcgtgccccc cacgagagca ccgtggcacc ggactcgtcc
     4561 gccggctcgg tcacgccgct gcgcgcgttc cgcaccggac tgctcaccaa cgtgctcaac
     4621 cccaaggtcg gggtggtcta catctcgctg ctgccgcagt tcatcccgca cggcgcgccg
     4681 gtcgtcgcga ccacggtcct gctggtcgcc gtgcatgccg cgctgggcgt gctgtggctg
     4741 ggcgggattg ccgtcgcggt gcaccgtgcg cgggccgtct tccagcggcc gggggtccgc
     4801 cggcggctcg atcaggccac cggcggagtg ctgctcgcgc tgggcgccgc ggtcggattc
     4861 gagaccgccc gctgacgggc cgcggcggtg tgcggcaccg cgggggaagc tcaaatcaag
     4921 ctctgaaccc ggccggtgcg gggttcgttc cgtcgcgacg gggcgatgag taccccgacc
     4981 acggagcgag agggggaggc atggagcgcc tggggacggg gatcggctgg cggccggaaa
     5041 tcgccgcaga catcgcggaa ctgccggggg tggactgggt cgaggtggtg gcggagaacc
     5101 tctgtccggg ccatctcccg cccgccctgc agcaattgcg tgagcgcggc acgaccgtcg
     5161 tgccgcacgg ggtctcgctc ggcctcggcg gcgcggaccg cccggacccc ggccggctga
     5221 ccgccctcgc ggaacgcgcc gaggcactgg gcgcgccgct ggtcaccgag cacatcgcgt
     5281 tcgtacgggc cggcgggccg ctgaccgcct cgccggtgat ggaggccggg cacctgctgc
     5341 ccgtaccgcg gacccgggat gcgctggacg tgctctgcga gaacgtccgg atcgcccagg
     5401 acgcgctgcc ggtgccgctg gcactggaga acatcgccgc cctggtcaac tggcccggcg
     5461 aggagatgac cgaaggccag ttcctgtccg agctggtcga gcggaccggt gtgcggctgc
     5521 tcatcgatgt cgccaacctg cacaccaacc acgtcaaccg gggcgaggac ccggccgtcg
     5581 cgctggacga gctgccggtc gaggccatcg cgtacgtcca tgtcgcgggc ggggtggagc
     5641 gggacggggt gtggcacgac agccacgccc acccggtgac ccagccggtg ctggacgtgc
     5701 tcgccgcgct gtgtgcccgc gtcaccccgc ccggggtgct gctggaacgg gacgacgact
     5761 tcccggaggg ccccgagctg gccggcgaac tggacgcgat ccgcacggtg ttgagggagg
     5821 cccgtcatgc gtacgcggct tgaggcggta cgggaggcgc ccgtacggga gaccccggca
     5881 gtggagtcgt ccctgccgga accgtccgta ccggagacgg ccgtacccga gacggccgta
     5941 cgtgagtcgt ccgcagccgg ggcgccctcc tcccggacac ccgccaccgg cacggacgcc
     6001 ggtgccgacg ccggcaccga cgccgctcgt cagcggctcg ccctggcgca ggccgcgctg
     6061 ctgtccgcgc tggtcgccgg gacgccggcg cccgagggct tcgaccgcac ccggcttcag
     6121 gtgcagagcc aggcgctgac cgccaagcgg gcctcggtgg tcgccaaggt ggcgcccgaa
     6181 ctgcccgtga tcctcggtgc ggactaccgg acggcgttcc tcggctacgc ccgtggccgt
     6241 ccgatgcgcg gcggctaccg ccaggacgcc ctggacttcg cggcgcacct gctcgcccag
     6301 gacctgcccg cggacgcggc cgcgcggcag cagttgacgc gctggtggcg ggaccgggcg
     6361 ggcccccggc cgccgtcctc ccggcccgtc acccgtctga cgcgcgccct gtgcgacggc
     6421 ctgcacgacg tcctgcgcag cgttctgcgg cggaggtgac cgggatgtcc ttcgcagagt
     6481 gggtgtactt ggggctcggc gtctcgtcgc tggtgctcgt gatcggcacg gtcgtgctcc
     6541 ggctgcggac gccgtccgtc cgccgggcgg acgcgctggc gcacgacgtc tgggagacgg
     6601 ccttcctcgc cgggggcccg gcgcgggtgg tggacgcggc ggtcgccggg atgcacgagg
     6661 acggccggct ggcggtgggc ggcccgggcg tggtgaccgt acgtcaggcc accgcccggg
     6721 acgcggtcga ggcggccgtg ctggacgcgg tcgcccggac gcccggcggg gcgctggccg
     6781 cgctgcgcgc cgaggcgatg cgcagcccgg ccgtccagga cgtcggcgac cggctggcgg
     6841 cgcggggcct gatgcgtcac ccgcggctcg gccggggctg gctcctgtgg gcgcgggcgc
     6901 accaggcggc gtgcggggtg ctcttcgtcg tcggcatggt gctctcggtg cccggcgacg
     6961 acgggaacga cgggcagacg gtcgtgatga tcctgccggc gctgatcggt gggctggtgg
     7021 tggggtcgct gtgccggaag gcgttccggc gccggatcac cagggcgggt tccgcggcgc
     7081 tgcgctccgc gcgggcggcc cacccccagg gggacgtggg cggcgcgtgg caggccgccg
     7141 ggctggtggt ggcgctcggc ggcaccgcgc tgctcgcgga cgagctgctg cggcagcagt
     7201 tcgaggaggc gcaccgggcg agcgcgctgg ccgcctcggg cgccgggtcc tcgtcgtccg
     7261 gcggctcgtc gtccggcttc gactccggtg gggggtcggg ggacgggggt tcctggtgcg
     7321 gctcgtcgga cggtgggtcc ggatcgagtt gcggcggttc gtcctgcggc gccggttcgt
     7381 cgtgcggggg cggttcgtca tgtggcggtg gctcgtcgtg cggcggcgga tcgagttgcg
     7441 ggggcggttc gggctgcggc ggcggctgag cccggatgcc gccggcgacc gcggcgggcg
     7501 cggcgggcgg acccggtccg gctggaggcg accgtcaccg ccgggcgggc ccggcgacag
     7561 tcgacgggcg gcgccccgac gacttcgtcc acgcccgcgc ccgcatgttg agaaacgaat
     7621 ggcgattgtc atgtctcatc ccgtaaaaat ctgccatgtg gctcctcttc ctcctcgtcg
     7681 cctgcgtggc ggcggcgctg agctgtgcgc agctctgccg tgctgccgtg gccgtcgccc
     7741 gcgccaccgg cccggccccg cggccggcac ccgcccccgt cccggacacg gccgccccgg
     7801 acggctccgc ggcgggtctg acgctgtatg agatggcgta tctctccggc ggcccgcacc
     7861 ggctggccga tgtggtgctg gtgctcatgg cgcagcagcg gcgcctgttg ctcgcccaca
     7921 ccggctgggc caccgtcgtc gatccggtcg gccaggaccc cgtcgagcgg gccaccctca
     7981 gcgccatagg cccggacggg cagcgccgga taccggcgat acgggaggcc ctggtggccg
     8041 acgaggcggt gcgggcggtc ggcgaccgac tggtggccgc cgggctcgca ctgcccgccg
     8101 cggcccgtgg tctgcgcaac gccgtccgcc aggtgcaggg cgcgacggtg ctggcgctgc
     8161 tctgcgccgc ggcggcgtac gcgacggcgc cggccggcga ggaccgggga cagctgctga
     8221 tctggttcac gctcccgctg atcctcaccc tggggacgct ggcggtggcg cgcttcgagc
     8281 tccatccgta cagcgactgg gcgaccgacg cgggcgagga acggctgccc ggcagacccg
     8341 gatttaccac gttcaccccc agctcccccg cccctgtcgc ccctctcgcc tccctgatga
     8401 cccttgccct ccacggaccc agggccctca ccgatcccgc cctgcgcgcg gcgctccact
     8461 cttccggggc atagcgtccg acaccggcat ccggtgcttt acatcgccaa ccaccgaccc
     8521 aaatatccct tttgtatgta caacacgaag ggacggccag tgagagcact tgcgttgtac
     8581 ggcaccatcg ggtcgctgat cgtgaccgcc ctcgccaccg ccccggcggg cggtgccacg
     8641 gaggcgcccg atgcgccccc ggcgcccgtg gcgcaccgga tcggcttcgg ccggtgcgct
     8701 ccggtggagc agctgccggc gtccgtcgag tgcggcaagc tggccgttcc cctggactac
     8761 gcgcggcccg acggcaagaa gatcgagctc accgtgagcc ggatccgcgc caccgtgccc
     8821 ggtgaacggc agggctccct ggtcttcaac cccggcgggc ccggcgcgtc cagccttgag
     8881 ttcccgctct acggcggcct gccaaagtgg agcaaggtgg cccgcgcgta cgacttcgtc
     8941 ggctacgcgc cgcgcggcgt cggccgctcg gctccgctgt cgtgccagga ccccaaggag
     9001 ttcgccaagg tcccgaccga cagccggatg cacccggacg cggccttcaa gcgcgcgaag
     9061 gtggcccagg cgagggcgta cgcccggggt tgcgcccgcc gggccggggc ggacctgccg
     9121 ttctacaact cggtcaacaa cgcccgcgac ctggacatgc tgcgggccgc gctgggcgag
     9181 aagaagctga cgtacatggg cgcctcgtac gggacgtact tcggggcggt ctacgcgacg
     9241 ctcttccccg gccacgtccg gcggatgatc ttcgacagtg tggtgaaccc cgagccgcgg
     9301 cagatctggt accgcaacaa cctcgaccag aacatcgcct tcgagcggcg ctggggcgac
     9361 tggctgcgct gggtcgccaa gcacgacgac cactaccacc tcggcgcgac ccggcaggcg
     9421 gtgcagcggg cctacgacca ggccgccgcc aagctgcgcc gcgacccgct cgacggcaag
     9481 atcggccccg gtcagctgca gtcggccttc ctgaagaccg gctacaacga cgccttctgg
     9541 ccgatgcgcg ccgaggcgct ctccggttac ctgcacggcg accccaaggc gctgatcgcc
     9601 caggccctgc cgcagtcgtc cggcgccagg gacgaggaga acgccaacgc ggtctacacg
     9661 gccgtggagt gcaacgacgg gccctggccg cgttcgtggc agacctggga ccgggacaac
     9721 accgaggtcg cccgcatcgc cccgttcgag acctgggaca acgcctggat gaatctgccg
     9781 tgcgcgttct ggcccgaccg gtcggagtcg gccgaggccg gtgtcgagca ggcggcccgc
     9841 cgggcggaac acggcaacgg gcaggagctg gcggacgcca ccggccacgc cgcgcggacc
     9901 gccatggacg gggccctgca cggcccgcgc ggccccctcg acgtgcacac cgagcccggc
     9961 gcgctgccgc cggtgctgct gctggcggcc gagcgggacg cggcgacacc gtacaagggc
    10021 gcggtggagc tccagcaccg gctgcccggc tcctcgctgg tcaccgagaa cggtgcgggc
    10081 acccacggcg tcgccttcaa cgacaacgcc tgtgcgacca ggtacgccga ggcgtacctg
    10141 ctccagggga agatcccgtc gcaccgggtg tactgcgcgc cgcgtgccga gccggtgccg
    10201 gggcagaagg cggtccgggc gcagctcgcc cggccgggga agtagaccgc ggagcgacgg
    10261 accgcgggct cgaccggccc gcacgacccg aaacgggccg cccctggggg cggcccgttt
    10321 tccgtgccga caggtcaggc cagaccggcc accagctccg ccaccggctt gcgccggccg
    10381 gtgtagaacg ggatctcctc acggacgtgc atccgcgcct cggagccgcg caggtgacgc
    10441 atgaggtcga cgatgcggta cagctcgtcg gcctcgaagg cgagcaccca ctcgtagtcg
    10501 ccgagcgaga acgacggcac ggtgttggcg cgcacgtccg ggaagccgcg ggccatcttg
    10561 ccgtggtcgg cgagcatccg gcggcggtcc tcgtcgggga ggaggtacca ctcgtaggag
    10621 cgcacgaagg ggtagacgct cacgtagttg cgcggctgct catcggccag gaaggccggg
    10681 atgtgcgact tgttgaactc cgcggggcgg tgcagcgcca tgttggacca caccggcgcg
    10741 agcgcgcggc cgagccgggt gcggcggaag aggttgtacg cctcctggag ggcgtccgag
    10801 ctctccgagt gccaccagat catgaggtcg gcgtcggcac gcagaccgga cacgtcgtac
    10861 gtgccgcgga ccaccacgtc cttggcggcg agctgcgcga acagctcctc gacctcgtcg
    10921 gcgtagccgc tgcggtcctc ggggaggacg tcgcgcagct tgaacaccga ccacagggtg
    10981 tagcggatga cctcgttgag gtccttggcc ttcttgccgg cgttcggagc cttctcgggc
    11041 gccgcggggg cctgcggcgc ggaagcggga gtggaagcgt ctgtcatgtc gtccattctc
    11101 actcgccggg gacggtggcc gggcgcaggg tgccgaggat gtcgtcggcg gccccgcggg
    11161 cggaggcgat gcaggccggg atgccgacgc cctcgtacag cgcgccgcac acccgcaggc
    11221 cc
//
