LOCUS       NZ_CP017157            10954 bp    DNA     linear   CON 31-MAY-2024
DEFINITION  Streptomyces lydicus strain 103 chromosome, complete genome.
ACCESSION   NZ_CP017157
VERSION     NZ_CP017157.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces lydicus
  ORGANISM  Streptomyces lydicus
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 8201357)
  AUTHORS   Jia,N., Ding,M.-Z., Gao,F. and Yuan,Y.-J.
  TITLE     Complete genome sequencing of Streptomyces lydicus 103 and metabolic
            pathways analysis of antibiotic biosynthesis
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8201357)
  AUTHORS   Jia,N., Ding,M.-Z., Gao,F. and Yuan,Y.-J.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2016) Key Laboratory of Systems Bioengineering
            (Ministry of Education), Tianjin University, Tianjin 300072, PR
            China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SMRT Analysis v. 2.3.0
            Genome Coverage                   :: 100x
            Sequencing Technology             :: PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_001729485.1-RS_2024_05_31
            Annotation Date                   :: 05/31/2024 02:23:15
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,288
            CDSs (total)                      :: 7,195
            Genes (coding)                    :: 7,061
            CDSs (with protein)               :: 7,061
            Genes (RNA)                       :: 93
            rRNAs                             :: 7, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 47 of 134
            Pseudo Genes (incomplete)         :: 104 of 134
            Pseudo Genes (internal stop)      :: 11 of 134
            Pseudo Genes (multiple problems)  :: 26 of 134
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 01:52:31
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 4723884
            Orig. end                         :: 4734838
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP017157.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Source DNA is available from Prof. Ying-Jin Yuan.
            Annotation modified by submitter.
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10954
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="butyrolactone"
                     /region_number="18"
                     /rules="AfsA"
                     /tool="antismash"
     cand_cluster    1..10954
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="AfsA"
                     /kind="single"
                     /product="butyrolactone"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10954
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5954](+)"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5954
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
     misc_feature    complement(186..199)
                     /note="TFBS match to BldD, Development and antibiotic
                     global regulator, confidence: weak, score: 19.17"
                     /tool="antismash"
     gene            515..811
                     /locus_tag="SL103_RS20505"
                     /old_locus_tag="SL103_20505"
     CDS             515..811
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="SL103_RS20505"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="SL103_20505"
                     /product="hypothetical protein"
                     /protein_id="WP_164492732.1"
                     /transl_table=11
                     /translation="MIGENEVPGPFGCAGRRPQVEGVPMPVRSVCATERRAGILVRRHD
                     AGTGRHYLVDGVPVVAGPVLVTSRSRSSRYWTLSRRIAARAPPPARRGLPPLP"
     gene            complement(1274..3721)
                     /locus_tag="SL103_RS20510"
                     /old_locus_tag="SL103_20510"
     CDS             complement(1274..3721)
                     /GO_function="GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1005: Drug
                     resistance transporter, EmrB/QacA"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016574653.1"
                     /locus_tag="SL103_RS20510"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SL103_20510"
                     /product="MFS transporter"
                     /protein_id="WP_069570429.1"
                     /transl_table=11
                     /translation="MTRTTTDQLSGRTATTAAAPPVPDPGAGGHRRPRGGIVPVLAFSG
                     IVVAVMQTLLVPVIKDLPVLLDTAPSNATWVMTATLLAGAVSTPIMGRLGDLYGKRRML
                     LASLAIMVVGSLICGCTSDLIVMIVGRALQGFAMGAIPLGIGIMRDELPRERLGSAMGL
                     MSSSIGVGGGLALPAAALVAQHADWHALFFGAAGLGVLSILLTLLVVPETSVRAVGRFD
                     VAGAMGLSAGLVALLLPITKGSDWGWSSPTTLGLFGVAALILVLWGVMELRIGDPLVDL
                     RTTARREVLLTNLASITVGVAFYAISLVLPQLLQLPKSTGYGLGQSMVVAGLCVAPLGL
                     TMMFVAPLYARIAARRGPKVSLLLGMLVIGVGYGAGIGLMDAPWQTVIIAVVVGAGIGL
                     AYSSLPALIIGAVDPSETGAANGLNTLMRSIGTSVSSAVIGMVLAHMSRSVGPVTVPTM
                     AGFRVAFLIAAAAVLVGVVLASFLPSQRKVSRPTLVAQSTEDATAAPEEERLQAPSTPA
                     PAPAPVDVTPAAPVPTAVAPAAAATPADVPPAAGTLVQDRAAGFRGRVRYASGAPIAGA
                     SVTLIDRQGRQAGITTAGPDGSYAVDAPTAGGYLLTAAAPGHTPHAASATCRGAGTPTE
                     MDLILGGGRRLGGTLRGGGTADAPLADGSVVVTDAGGEVVARTATDAHGNWELSTLPPG
                     AHTLVLSAPGHRPEARAIELSGGEPARHDARLRPTATVRGTVRDPKGRPLADAAVTLVE
                     DGTVAGHTRTGQDGAFAFDDLGGSHYTLTAAGYPPHATPISLAAGVAETLDLDLEQPSG
                     VGG"
     misc_feature    complement(3781..3794)
                     /note="TFBS match to LexA_variant_2, Repressor of DNA
                     damage response, confidence: weak, score: 17.6"
                     /tool="antismash"
     misc_feature    3839..3854
                     /note="TFBS match to CRP, Cyclic AMP receptor protein,
                     confidence: medium, score: 16.26"
                     /tool="antismash"
     gene            complement(4134..4814)
                     /locus_tag="SL103_RS20515"
                     /old_locus_tag="SL103_20515"
     CDS             complement(4134..4814)
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1057: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003991562.1"
                     /locus_tag="SL103_RS20515"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SL103_20515"
                     /product="ScbR family autoregulator-binding transcription
                     factor"
                     /protein_id="WP_069570430.1"
                     /transl_table=11
                     /translation="MAQQDRAIRTRRVILEAAASVFDEQGYDRATIAEVLERAGVTKGA
                     LYFHFASKEQLALAVLEEQVVDIAVEPQKIKLQEFVDAGQVLAYRLRRDPIQRGAARLA
                     VEQGSNHLDRKQSMISWTRFVEGLLNEARERGEILESIVVRDTAELFVGAFAGLQMMSH
                     AMTNQADLSHRLTVFFEHTLPSIAVPAVLAKLNLDPERGEKLEAALRQKVPAAAEVGES
                     VAVS"
     misc_feature    4914..4927
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 15.3"
                     /tool="antismash"
     gene            5001..5954
                     /locus_tag="SL103_RS20520"
                     /old_locus_tag="SL103_20520"
     CDS             5001..5954
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     butyrolactone: AfsA"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006608008.1"
                     /locus_tag="SL103_RS20520"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SL103_20520"
                     /product="ScbA/BarX family gamma-butyrolactone biosynthesis
                     protein"
                     /protein_id="WP_244304182.1"
                     /sec_met_domain="AfsA (E-value: 8.6e-19, bitscore: 66.1,
                     seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MHVSGTVQPKAPHRTRVIEKPSDTPQVPREFVHRPIVDDILVTSW
                     RRQDGSHFSVTAQWPEEHGYFASHDGRHHLILTGETIRQAGLLLSHTELGVPVGHHFIL
                     GDLICTTYPEHLDAGSGPTPISIDVTCSNMRMRAGTLAGARFDMTLRAADRIVATGHSH
                     VTVASPAVYRRIRGERLTARRALGPLPSCVAPRLTGSASEHDVLLSPTDRPDSWLLRIA
                     PGHAAVVNPANDHVPGMVLLDAAQQAAHALTAPGTFVPYAFGTTFHRYAEHGTPCLIEA
                     RRVPSALPRTTTVQITGSQDGHPVFVSTLTARDSRG"
     gene            6109..6873
                     /locus_tag="SL103_RS20525"
                     /old_locus_tag="SL103_20525"
     CDS             6109..6873
                     /NRPS_PKS="Domain: PKS_KR (7-174). E-value: 3.3e-09. Score:
                     28.8. Matches aSDomain:
                     nrpspksdomains_SL103_RS20525_PKS_KR.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1001: short-chain dehydrogenase/reductase SDR"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006608009.1"
                     /locus_tag="SL103_RS20525"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SL103_20525"
                     /product="SDR family oxidoreductase"
                     /protein_id="WP_069570432.1"
                     /transl_table=11
                     /translation="MGDLNGKTALVTGSSRGIGRGIAQRLARDGALVAVHYGNNDTAAK
                     ETAENIRGAGGRAFIIGAELGVPGDAESLFAAFDDGLAACGAEPGLDILVNNAAISLPG
                     HIDEVTPEEFDRTLAVNTKAPFFVIQHGLRRMRDGGRIINISSAVTTTAFPSTIAYGVS
                     KGAVDTLTLTLAKDLGARGITVNTIAPGFVATDMNAAMRATPEAEAALSAISVFNRLGR
                     PSDIADVAAFLASDDSRWITGQRFDVTGGSML"
     aSDomain        6130..6630
                     /aSDomain="PKS_KR"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_SL103_RS20525_PKS_KR.1"
                     /evalue="3.30E-09"
                     /label="SL103_RS20525_PKS_KR.1"
                     /locus_tag="SL103_RS20525"
                     /protein_end="174"
                     /protein_start="7"
                     /score="28.8"
                     /specificity="KR activity: inactive"
                     /specificity="KR stereochemistry: C2"
                     /tool="antismash"
                     /translation="TALVTGSSRGIGRGIAQRLARDGALVAVHYGNNDTAAKETAENIR
                     GAGGRAFIIGAELGVPGDAESLFAAFDDGLAACGAEPGLDILVNNAAISLPGHIDEVTP
                     EEFDRTLAVNTKAPFFVIQHGLRRMRDGGRIINISSAVTTTAFPSTIAYGVSKGAVDTL
                     TLTL"
     CDS_motif       6136..6201
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_SL103_RS20525_0001"
                     /evalue="1.20E-03"
                     /label="PKSI-KR_m1"
                     /locus_tag="SL103_RS20525"
                     /protein_end="31"
                     /protein_start="9"
                     /score="11.6"
                     /tool="antismash"
                     /translation="LVTGSSRGIGRGIAQRLARDGA"
     gene            complement(7049..7816)
                     /locus_tag="SL103_RS20530"
                     /old_locus_tag="SL103_20530"
     CDS             complement(7049..7816)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006608010.1"
                     /locus_tag="SL103_RS20530"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SL103_20530"
                     /product="2OG-Fe(II) oxygenase"
                     /protein_id="WP_069570433.1"
                     /transl_table=11
                     /translation="MTATTNHRAGTHPPAGPARRSAPFAARVAATDWAALGEELDAVGC
                     ALTPRLLTPADCRALIARYDEAERFRSTVDMARHRFGSGQYRYFADPLPEPVRALREAF
                     YPHLLTVARAWADKLGRPAPWPDSLAEWLRMCHAAGQTKSAQILLRYGAGDWNALHRDL
                     FGERVFPLQVVIGLDEQGTDYEGGEFLLVEQRPRAQSRGTTTVLPQGHGLVFTTRDRPV
                     RSARGWSAGAVRHGVSTVRDGRRHSLGLVFHDA"
     gene            complement(7813..8340)
                     /locus_tag="SL103_RS20535"
                     /old_locus_tag="SL103_20535"
     CDS             complement(7813..8340)
                     /EC_number="2.1.1.63"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006608011.1"
                     /locus_tag="SL103_RS20535"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SL103_20535"
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="WP_069570434.1"
                     /transl_table=11
                     /translation="MTLYTTLDSPLGELLLVGEESATAPGGTALVSLSVPDQKGGVTVQ
                     DGWQHAPRAFAEITRQLRAYFDGTLTRFGIEYANTASTRGTDFQQRVWQALESIPYGTT
                     TTYGRIAADIGAARGAVRAVGTAIGANPLLVVRPCHRVIAANGALSGYAGGPARKRQLL
                     GLEGVVPNGSTS"
     gene            complement(8596..8757)
                     /locus_tag="SL103_RS20540"
                     /old_locus_tag="SL103_20540"
     CDS             complement(8596..8757)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019548361.1"
                     /locus_tag="SL103_RS20540"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SL103_20540"
                     /product="hypothetical protein"
                     /protein_id="WP_164492871.1"
                     /transl_table=11
                     /translation="MSDGDSGDDDAGAGTARGSRGGGLPSWDVLRRAMRWETLAVPGFL
                     VLLAAPAS"
     gene            complement(9010..9237)
                     /locus_tag="SL103_RS20545"
                     /old_locus_tag="SL103_20545"
     CDS             complement(9010..9237)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006608014.1"
                     /locus_tag="SL103_RS20545"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SL103_20545"
                     /product="hypothetical protein"
                     /protein_id="WP_069570436.1"
                     /transl_table=11
                     /translation="MTEHPPVIVHPPTPSGGRRVTVRGQIVGLAHGRGDVAEFLRRAGV
                     AGPAEDIRLDDPRLVEWRGGSLNDWPMPPA"
     gene            9332..10540
                     /locus_tag="SL103_RS20550"
                     /old_locus_tag="SL103_20550"
     CDS             9332..10540
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) p450"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1007: cytochrome P450"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019074505.1"
                     /locus_tag="SL103_RS20550"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SL103_20550"
                     /product="cytochrome P450"
                     /protein_id="WP_069570437.1"
                     /sec_met_domain="p450 (E-value: 1.4e-22, bitscore: 78.8,
                     seeds: 50, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MAPNPLESAEFSRDPYPLLAALRTQGAVQRMPTGDGRTTWVVTGW
                     PEARAALADARLSKDTARYFADRPSNRRLAPAVGQSMLATDPPRHGRLRKLAMAAFTPA
                     AVARLAPRVRALAEELADALAARGSGDLIEEFAVPLPIAVISELLGVPEAERAAVRAWS
                     NDLFAAAAPETVDRASHALSACMTRLIASRRAAPSDDVLSGLIAARDEADRLSEAELVS
                     LAVLLVVAGHETTTHLIGNGMLALLKDDALRARLRAEPALLPAAVEEFLRHDAPLTLAT
                     FRYATVPVELGGARIAAGEVVLVSPAAANRDPARFAAPDEVRLDRPDQAGRHLSFGHGP
                     HHCLGAPLARLEARIAFEVLLTRFPDIRPASVSWDELEWRRTRLMRGLVRLPVHVDRRA
                     IDR"
ORIGIN
        1 ccggcgcccg gggccggcga tccggcccgc agccgcacgt cgtacggggc gaggaaccgg
       61 gggtcaccgc gtaccgcgcc ggcgtcggtg gcgggcaccg cggagatccg ggcgtagagg
      121 ttgtggtcgt aggtgtggtg tgcgtcggca ggcggggagc cgcccgcggc gccggcgaag
      181 acgtgtcacg gaaggtgacg ccccgggacc gttgctgctg accacggccg tgccagggac
      241 gttcgtggtg acggtgttgt ggtgcacaag agtacgagtg gcggacgcac agacgaccga
      301 aatgacgcca tagggcgcgg tggtgttgcg gtcgttgaca ctgacgttgt agcggacggt
      361 gttgcggtcg gagatcatgc cggcgttgta gccggcggag cgggcgttgt agccgtcgac
      421 gaggtggtgc tcgacccgcg ccccgaccgc gttctgcacg acgatcccgt ctcccccggc
      481 gtcccgcacg gtgttgtggt gcaccgacag cccggtgatc ggcgagaacg aggtgccggg
      541 cccgttcggg tgcgcgggcc gccggcccca ggtcgagggg gtcccgatgc cggtgcggtc
      601 ggtgtgcgcc accgagcggc gcgccgggat cctcgtacgg cggcacgacg ccggaaccgg
      661 ccggcactac ctggtggacg gcgtgccggt ggtggccggc ccggtgttgg tcacgtcgag
      721 gtcgcgcagc tcccggtact ggacgttgtc gaggaggatc gccgcccggg ccccgccgcc
      781 cgcgaggcgc ggcctgccgc cgctgccgta ggcgcccgcg gcgcggggcg cgcccgcggc
      841 gccggacgcg gcgggcgcga aggtgccggt gcaagtggtg ccgcgccgga agaggatccg
      901 gtcactgggg cggtccgggg gccggggccg gccgggccgg tggggcggcc agagccgcgg
      961 tcgcgggcag ggcggcgacg agtcgaggga cggcgctcat ctctcccctt tcggtgcacc
     1021 ggcgtgtcgc ggggcccgcc tccggaccgt gcgacacgcc ggtgatgagg tgccggtggc
     1081 ggccacatcg ttgggcagcc gccgacggga ggcaaggcac agcggacgaa agtgcaagcg
     1141 ctgccctgga aagggcaact cggggcgggc gacgccgagg cggcgagccg ggcgcggcca
     1201 ggccgaactc gacgagatgc cggggcggtt gacgaagagg ggcagttgac gaagagggcg
     1261 ggccccggga gggtcagccg cccacaccgc tcggttgctc caggtccagg tcgagagtct
     1321 cggcgacgcc cgcggcgagg gatatcgggg tggcgtgcgg cggatagccg gccgcggtca
     1381 gggtgtagtg gctgccgccc aggtcgtcga aggcgaaggc gccgtcctgg ccggtgcggg
     1441 tgtgcccggc gaccgtaccg tcctcgacga gggtcacggc ggcgtcggcc agcggcctcc
     1501 ccttcgggtc ccggacggtg ccgcggacgg tggcggtggg gcgcagccgg gcatcgtgcc
     1561 gggcgggctc accgccggac agttcgatgg cccgggcctc cggccggtga ccgggggcgc
     1621 tgaggaccag ggtgtgtgcg ccgggcggca gcgtgctcag ttcccagttc ccgtgcgcgt
     1681 ccgtggccgt acgggccacc acctcgccgc cggcgtcggt gacgacgacg cttccgtcgg
     1741 ccagtggtgc gtccgccgtc cctccaccgc gcagggtgcc gcccaggcgg cgcccaccgc
     1801 cgagtatcag gtccatctcg gtcggtgtgc ccgcaccgcg gcaggtggcg gaggccgcgt
     1861 gcggggtgtg gccgggggcg gcggccgtga ggagatagcc gccggcggtc ggggcgtcca
     1921 ccgcgtaact gccgtcgggg ccggccgtgg tgatgcccgc ctgccgtccc tgccggtcga
     1981 tcagcgtcac gctcgcgccc gctatcggcg cgccggaggc gtaccgtacg cggccccgga
     2041 acccggcggc gcggtcctgt acgagggtcc cggcggcggg gggcacgtca gccggggtcg
     2101 cggcggcggc cggggccacc gcggtcggca ccggggcagc cggcgtcacg tcgaccgggg
     2161 ccggggccgg ggccggggtg gacggtgcct ggagacgctc ctcctcgggc gccgccgtcg
     2221 cgtcctcggt gctctgcgcg accagggtcg gccgggagac cttgcgctgc gacggcagga
     2281 acgacgccag gaccacgccg accaggaccg cggcggcggc gatcaggaag gcgacccgga
     2341 agccggccat cgtggggacc gtgaccggtc ccacgctccg ggacatatgg gccagcacca
     2401 tgccgatcac cgcgctggag acggaggtgc cgatcgagcg catcagggtg ttgaggccgt
     2461 tggcggcgcc ggtctcggac gggtcgacgg cgccgatgat cagtgcgggc agcgaggagt
     2521 acgcgaggcc gataccggcg ccgacaacca ccgcgatgat gacggtctgc cagggcgcgt
     2581 ccatcaggcc gatgcccgcg ccgtagccaa cccctatgac gagcatgccg agcagcaacg
     2641 agaccttggg gccgcgccgc gcggcgatcc gcgcgtacag cggcgcgacg aacatcatcg
     2701 tcaggccgag cggtgccacg cacagaccgg ccaccaccat cgactgaccg aggccgtagc
     2761 cggtcgactt cggcagttgc agcagctgcg gcagcaccag cgagatggcg tagaacgcca
     2821 ccccgaccgt gatcgaggcg aggttggtca gcagcacctc gcggcgggcg gtggtccgca
     2881 gatcgaccag cgggtcgccg atgcgcagct ccatcacgcc ccacagcacc aggatcaggg
     2941 ccgcgacacc gaagaggccg agcgtggtgg gcgaactcca cccccagtca ctgcccttgg
     3001 tgatgggcag cagcagggcc accagcccgg cggacagccc catggcaccg gccacatcga
     3061 agcgccccac ggcacgtacg gaggtctcgg ggacgacgag gagggtgagc agtatcgaca
     3121 gcaccccgag accggcggcg ccgaagaaca gggcgtgcca gtcggcgtgt tgggcgacca
     3181 gcgccgcggc cggcagcgcg agcccgccgc cgacgccgat cgacgagctc atcaggccca
     3241 tggccgagcc gagccgctcg cgcggcagct cgtcacgcat gatgccgatg ccgagcggga
     3301 tggcacccat ggcgaagccc tgcagcgcgc ggcccacgat catgacgatc aggtcactgg
     3361 tgcatccgca gatcagcgag ccgacgacca tgatcgcgag gctcgccagc agcatccgcc
     3421 gcttgccgta gaggtcgccg agccgcccca tgatgggtgt cgagacggcg cccgcgagca
     3481 gggtggccgt catgacccag gtggcgttgc tgggggcggt gtcgagcagc accggcaggt
     3541 ccttgatgac cggcacgagc agcgtctgca tgaccgcgac gacgatgccg gagaaggcca
     3601 ggaccgggac gatgccgccg cggggtctgc ggtggccgcc cgcaccgggg tcgggcaccg
     3661 ggggcgccgc ggcggtggtc gccgtccttc cggacagctg gtccgtcgtc gtccgtgtca
     3721 tacgtgcggc ctccagggcg gcaggggtgg gtgcggcagg cgccatcgat acgtgcatgg
     3781 cgaacgtgtt ttccccgtgc cctattccgc ggatcggcga gcgccgcacg ttcttgacgg
     3841 tgatctccct cacgccgccc ccccggtccc gacgtcgatg ccgaccccgt cggaccggcc
     3901 aactccagtg aagcgcacgc cactgacaat cccgccggca ccggcggccc cacagacaag
     3961 aggggcgccg cggtgcaccg cggcgccctc accccctggc cgacccgtct caactgagcc
     4021 gccggcccct gtcgtgctca gcaacaacac cgcgtgcgcg gtatgtttgc cgcccccaaa
     4081 tccccctgaa tcacacaaat cacccggtgc cccgctgcgg tgcgacccgg cggtcagctg
     4141 acggcgacgc tctccccgac ctcggccgcc gccggcacct tctgccggag agcggcctcc
     4201 agcttctccc cgcgctcggg gtcgagattg agtttggcga gcaccgcggg caccgcaatg
     4261 ctggggaggg tgtgctcgaa gaagaccgtc aaccggtggc tcaggtcggc ctgattggtc
     4321 atcgcgtgcg acatcatctg gagcccggcg aacgcaccga cgaacaactc ggcggtgtcg
     4381 cgtacgacga tgctctccag gatttcgccg cgctcccgcg cctcgttcag cagcccctcg
     4441 acgaaccgcg tccacgagat catcgactgc ttgcggtcaa ggtggttcga cccctgctcg
     4501 accgccagcc gggcggcccc gcgctggatc ggatcgcggc gcaggcgata ggccagcacc
     4561 tgccccgcgt cgacgaattc ctgcaacttg atcttctgcg gctcgacggc gatgtcgacg
     4621 acctgctctt cgagtacggc gagtgcgagc tgttccttcg aggcgaagtg gaagtacagg
     4681 gcgcccttcg tcaccccggc gcgctccagg acttcggcga tcgtcgcgcg gtcgtagccc
     4741 tgctcgtcga aaaccgacgc ggccgcctcc aagatcactc ggcgggtccg gatcgcgcgg
     4801 tcctgttgtg ccacaggacg cctcctgtct ctcgaagtct tcaagttccg caactgttcg
     4861 ctacagctca agtcgccgct cgaccagcct atttcccctt tccaccggcc cggcattgaa
     4921 aataaaaccg gatggtacgt atcttaacag cgatggacct cattccgtag agacgcctgg
     4981 gggcagtcat gagcgaaacg atgcacgtca gcggcacagt tcagcccaag gcaccgcatc
     5041 ggacgcgcgt gatcgaaaag ccctccgaca ccccacaggt cccccgcgaa tttgtgcacc
     5101 gcccgattgt tgatgacatt ctggtcacat cttggcgccg acaggacggc tcccacttct
     5161 cagtgaccgc ccagtggccc gaggagcacg ggtacttcgc ctcccatgac gggcggcacc
     5221 acctcatcct caccggtgag accatccgtc aggccggatt gctgctgtcg cacaccgaac
     5281 tcggcgtccc ggtcggccac cacttcatcc tcggggacct catctgcacc acgtatcccg
     5341 aacacctcga cgccggcagc gggcccaccc ccatctcgat cgacgtcacc tgcagcaaca
     5401 tgcggatgcg ggccggcacg ctggccggtg cccgcttcga catgacgctg cgcgccgcgg
     5461 accggatcgt ggccacgggc cactcccacg tcaccgtcgc ctcgccggcc gtctaccgtc
     5521 ggatccgcgg cgaacggctc accgcccgtc gggccctcgg ccccctgccg agctgcgtgg
     5581 cgccccggct gaccggcagc gccagcgagc acgacgtact gctctccccc accgaccggc
     5641 ccgacagctg gctgctgcgc atcgcgcccg gtcacgccgc ggtcgtgaac ccggccaacg
     5701 accacgtccc gggcatggtg ctgctcgacg cggcacagca ggccgcgcac gccctgaccg
     5761 cgcccggcac cttcgtgccg tacgccttcg gcaccacgtt ccaccgctac gccgagcacg
     5821 gcaccccgtg cctgatcgag gcccgccggg tcccgtccgc cctgccccgc accacgacgg
     5881 tgcagatcac cggctcgcag gacggccacc cggtgttcgt gtcgacgctg acggcccggg
     5941 attcccgcgg ctgaccggac acctggcccg cagcgccgca gacccggcgc gccgcacgcg
     6001 tcacgcgacc cttccgttgc ctcggtacgc gcgtgccgcc cttcgcaccg cattcgacag
     6061 tcaacgcggc gacctgccgc gacagttaac ggaaggccgg aaacaatcat gggtgacctc
     6121 aacggaaaga ccgcgctggt gaccgggtcc agccggggca tcggccgggg catcgcccaa
     6181 cgcctcgccc gggacggcgc gctggtcgcc gtccactacg ggaacaacga cacggcggcc
     6241 aaggagacgg cggagaacat cagaggagcc ggcggccggg ccttcataat cggcgccgaa
     6301 ctgggagtgc cgggtgacgc cgagagcctc ttcgccgcct tcgacgacgg gctcgcggcg
     6361 tgcggggcgg agccgggcct cgacatcctc gtcaacaacg ccgccatcag ccttccgggg
     6421 cacatcgacg aggtgacgcc ggaagagttc gaccgcaccc tcgcggtcaa caccaaggcg
     6481 ccgttcttcg tcatccagca cggtctgcgc cggatgcgcg acggcggacg catcatcaac
     6541 atctcgtcgg cggtgacgac cacggccttc ccgtcgacca tcgcgtacgg cgtctccaag
     6601 ggcgcggtcg acaccctcac cctcaccctc gccaaggatc tcggcgcccg cggcatcacc
     6661 gtgaacacca tcgcgccggg cttcgtcgcc accgacatga acgccgcgat gcgggcgacg
     6721 ccggaggccg aggccgcgct gtccgccatc tccgtcttca accgcctggg ccggccctcg
     6781 gacatcgccg atgtcgccgc gttcctcgcc tcggacgact cccgctggat cacgggccag
     6841 cgcttcgacg tcaccggcgg ctcgatgctc tgagcgccgc gcgtccccgg gccgcgggcg
     6901 cgggagtcgt cgtcgggctt cgcgcagacg tcgtagacct ggtcggacgt cgtagacctg
     6961 gtgggacgtc gtagacctgg tcggacgtcg taaacctggt cggacgtcgt agacctggtc
     7021 ggacgtcgtc gggcgccggc aagcgtcctc aggcgtcgtg gaagaccagg cccagggagt
     7081 gccgacgacc gtcgcgaacg gtgctcacgc cgtggcgtac ggcgcctgcc gaccagcccc
     7141 gtgcagagcg cacggggcgg tcgcgggtgg tgaagaccag accgtggccc tggggcagga
     7201 cggtggtggt gccgcgggac tgggcccggg gccgctgttc gacgagcagg aactccccgc
     7261 cctcgtaatc ggtgccctgc tcgtccaggc cgatgacgac ctggagcggg aagacccgct
     7321 cgccgaacag atcacggtgc agtgcgttcc agtcgcccgc cccgtaacgc agcaggatct
     7381 gcgcggactt ggtctggccg gccgcgtggc acatccggag ccactccgcc agcgagtccg
     7441 gccagggggc cggcctgccg agcttgtccg cccaggcccg ggcgacggtc agcaggtgcg
     7501 ggtagaacgc ctcgcgcagg gcccggaccg gttcgggcag cggatctgcg aagtagcggt
     7561 actggcccga accgaagcgg tggcgcgcca tgtctacggt cgaccggaag cgctccgcct
     7621 cgtcgtagcg ggcgatcagc gcgcggcagt cggccggggt gagcagacgc ggggtcaacg
     7681 cgcagcccac cgcgtcgagt tcctcgccca gcgccgccca gtcggtggcc gcgacacgtg
     7741 ccgcgaacgg ggcggagcgg cgagcggggc cggcaggcgg gtgcgtcccg gcgcggtggt
     7801 tcgtggtggc ggtcatgacg tgctcccgtt cggtacgacc ccctccaggc cgaggagctg
     7861 ccgcttgcgc gccggtccgc ccgcgtagcc cgagagcgcc ccgttcgcgg cgatcacccg
     7921 gtggcagggc cggacgacga gcagcgggtt ggcgccgatc gccgttccga ccgcacggac
     7981 cgcaccgcgc gccgccccga tgtcggcggc gatccgtccg taggtcgtgg tcgtcccgta
     8041 cgggatcgac tccagcgcct gccacacgcg ctgctggaag tcggtgcccc gggtgctcgc
     8101 ggtgttcgcg tactcgatgc cgaagcgggt cagcgttccg tcgaagtagg cccggagctg
     8161 gcgggtgatc tcggcgaacg ccctgggggc gtgctgccag ccgtcctgga cggtgacccc
     8221 gcccttctgg tccggcacgg acagcgagac gagcgcggtg ccgcccggcg cggtggccga
     8281 ctcctcgccc accagcagga gttcacccag cgggctgtcg agcgtggtgt agagcgtcat
     8341 ggtcgtgttc tttccgtggt gtcggggccc ggtgcctgcg ggggtgggcc gtacctgctg
     8401 cggtacagcc ggcgtccact gtcgcgcacc gcctcttacc tggcttttct cgtccttcac
     8461 aggtccactc tgcggcagtt cgacgagccg cgctggcgga aatcggacgg ggcgttcatg
     8521 gccgtgaaca cgcgggacgg gcgggagtgc gagacggtac gggtgcaggt ggctcggcgc
     8581 tgcggccacc cggcgtcagg acgccggcgc cgcgagcaac accaggaacc ccggcaccgc
     8641 cagggtctcc cagcgcatcg ccctgcgcag cacgtcccag ctcgggagcc cgcctccccg
     8701 actgccccgc gcggtccccg cgccggcgtc gtcgtcgccg ctgtcgccgt cgctcatgcc
     8761 cggcccccgg tctccggcat cctgcccctc ccacgtgtcc tcccccgtcg atcgctcgaa
     8821 cccctccatt gatacctgct gccactgaca tcgcgctcga cgccccagcg agcagcccgt
     8881 gacaccctcg cgccgggtcc cgtcacccct ggggcaccaa ccgttcgtca cccggcgcag
     8941 gaggtccggg tctcgtcggg ccgacgggat cggtccgcct cggcactccg gggggaccga
     9001 tcgagctcct cacgcgggtg gcatcggcca gtcgttcagc gaaccgcccc gccactcgac
     9061 gaggcgcggg tcgtcgagcc ggatgtcctc cgcggggccc gcgactcccg cgcggcgcag
     9121 gaattcggcc acatcgccgc ggccgtgggc caacccgacg atctgcccac gcacggtcac
     9181 ccgtcggcca cccgaggggg tcggcgggtg cacgatgaca ggcggatgct cggtcatgcc
     9241 tccagcgtgc gccgggcccc gcggtcgcgc acgtcgacag ccgccaccag cgcgatccta
     9301 gcgcccggca accactcgct acgctgcccg tatggcaccc aacccgctgg agtcggccga
     9361 gttctccagg gacccgtacc cgctgctcgc cgccctgcgg acgcagggag ccgtccaacg
     9421 gatgccgacc ggtgacggcc gtaccacatg ggtcgtcacg gggtggcccg aggcgcgggc
     9481 cgcgctggcc gacgcgcggc tgtcgaagga caccgcccgc tacttcgccg accggccgag
     9541 caaccgccgg ctggcccccg cggtcgggca atccatgctc gccaccgatc cgccgcgcca
     9601 cggaagactg cggaaactgg cgatggcggc gttcaccccg gcggccgtcg cgcggctcgc
     9661 gccccgtgtc cgcgcgctcg cggaggaact cgccgacgcg ctggccgcgc gcgggtccgg
     9721 ggacctcatc gaggagttcg cggtgccgct gccgatcgcg gtgatcagcg agctgctcgg
     9781 ggtaccggag gccgagcgcg cggcggtgcg cgcgtggtcc aacgacctgt tcgccgcggc
     9841 ggccccggag accgtcgacc gcgcctcgca cgcgctcagc gcctgcatga cgcggctgat
     9901 cgcgtcccgg cgcgcggccc cgagcgacga tgtgctgagt ggtctgatcg ccgcccgcga
     9961 cgaggccgac cgactgtccg aggccgaact ggtgtcgctg gccgtgctgt tggtcgtcgc
    10021 gggccacgag acgaccaccc atctcatcgg caacggcatg ctggccctgc tcaaggacga
    10081 cgcgctgcgc gcccggctgc gggccgagcc cgcgctgctg cccgccgcgg tcgaggagtt
    10141 cctgcgccac gacgcacccc tcaccctcgc gaccttccgc tacgccaccg tgccggtcga
    10201 gctgggcggg gcgcgcatcg cggcgggcga ggtggtcctg gtctcccccg ccgcggccaa
    10261 tcgcgacccg gcgcgtttcg cagcgcccga cgaggtacgg ctggaccgcc ccgaccaggc
    10321 cggccggcac ctctccttcg ggcacggccc gcaccactgc ctcggcgcac cgctggcccg
    10381 tctggaggcc cggatcgcgt tcgaggtgct gctgacacgt ttccccgaca tccgtccggc
    10441 gtcggtgagt tgggacgagc tggagtggcg gcggacacgc ctgatgcggg ggctggtgcg
    10501 cctcccggta cacgtcgacc ggcgagccat tgaccggtga tccgtcgacc agtgatccgg
    10561 tgtgcgcgac aatcaaggcc atgacaatgg atgatcttgt gatgccggaa accccggtct
    10621 gtcgcgccgc gttggaggtg gcgagcgcct actgctcccc cgcactgctg aaccactccg
    10681 tacgcgccta cgtctgggcg acggcctacg cgtcgctgca cgacatcgcc ttcgacccgg
    10741 agctgctgta cgtgtcagcg atgctccacg acatcgggct ggtcaaggag ttcgacagcc
    10801 acaccgtgcc cttcgaggag gcgggtggcc atgtggcgtg ggtgttcggc gcgggcgccg
    10861 ggtggccggt ggagcggcgg gtgcgggcgt ccgaggtgat cgtccggcac atgtgggacg
    10921 acgtaccggt ggaagcggac ccggaggccc atct
//
