LOCUS       NZ_CP017157            11935 bp    DNA     linear   CON 31-MAY-2024
DEFINITION  Streptomyces lydicus strain 103 chromosome, complete genome.
ACCESSION   NZ_CP017157
VERSION     NZ_CP017157.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces lydicus
  ORGANISM  Streptomyces lydicus
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 8201357)
  AUTHORS   Jia,N., Ding,M.-Z., Gao,F. and Yuan,Y.-J.
  TITLE     Complete genome sequencing of Streptomyces lydicus 103 and metabolic
            pathways analysis of antibiotic biosynthesis
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8201357)
  AUTHORS   Jia,N., Ding,M.-Z., Gao,F. and Yuan,Y.-J.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2016) Key Laboratory of Systems Bioengineering
            (Ministry of Education), Tianjin University, Tianjin 300072, PR
            China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SMRT Analysis v. 2.3.0
            Genome Coverage                   :: 100x
            Sequencing Technology             :: PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_001729485.1-RS_2024_05_31
            Annotation Date                   :: 05/31/2024 02:23:15
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,288
            CDSs (total)                      :: 7,195
            Genes (coding)                    :: 7,061
            CDSs (with protein)               :: 7,061
            Genes (RNA)                       :: 93
            rRNAs                             :: 7, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 47 of 134
            Pseudo Genes (incomplete)         :: 104 of 134
            Pseudo Genes (internal stop)      :: 11 of 134
            Pseudo Genes (multiple problems)  :: 26 of 134
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 01:52:31
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 4574347
            Orig. end                         :: 4586282
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP017157.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Source DNA is available from Prof. Ying-Jin Yuan.
            Annotation modified by submitter.
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..11935
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="17"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..11935
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..11935
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:6935](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..6935
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     misc_feature    complement(1300..1313)
                     /note="TFBS match to RpoN, Regulator of nitrogen
                     assimilation and virulence, confidence: medium, score:
                     16.82"
                     /tool="antismash"
     misc_feature    complement(1447..1462)
                     /note="TFBS match to CRP, Cyclic AMP receptor protein,
                     confidence: weak, score: 15.73"
                     /tool="antismash"
     gene            1560..2834
                     /locus_tag="SL103_RS19810"
                     /old_locus_tag="SL103_19810"
     CDS             1560..2834
                     /GO_function="GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1020: major
                     facilitator transporter"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018088740.1"
                     /locus_tag="SL103_RS19810"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SL103_19810"
                     /product="MFS transporter"
                     /protein_id="WP_079145868.1"
                     /transl_table=11
                     /translation="MTSSAAGAAASAVPLPGARRDVRLFWWAGTCDALGSQVSGLVLPL
                     LLLSLGWSPAEVGVVAGLSAAATLLLGPLAAVPADRGARKSVMVGAAVVSALAMACVAV
                     TVGRETVLPAVLLTAVLVERCATSCYEAASLGTVALVSPPGEHRRVLSRLQAGERGALV
                     LGPALGGLLFHTGRWLPFLADALSYAVAAGCIRAMRADLAPAREHGSPAGTPRRGPRAL
                     AAEAGAGVAVIRRDPFLRLAFVWLTAVNALLVALYYTTVFALQGGGRGAAALGLVLALA
                     GAAGLGGALLAPALAGRHSARRVLVPVSWLLVPPAAALATAREGWQFGLLFGLLCVLTP
                     LATVALQARVIQVTPPALQARTGTVLATAAGAGAALAPALAGLAADRAGAAVTFTGCAL
                     LLAVLALYATCTAPGLPRADAEGAA"
     misc_feature    2019..2021
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            2831..4801
                     /locus_tag="SL103_RS19815"
                     /old_locus_tag="SL103_19815"
     CDS             2831..4801
                     /GO_function="GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /GO_process="GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Peptidase_S9"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020129145.1"
                     /locus_tag="SL103_RS19815"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SL103_19815"
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="WP_069570309.1"
                     /sec_met_domain="Peptidase_S9 (E-value: 1.7e-34, bitscore:
                     117.9, seeds: 64, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSAGDFRVYQPALPEVCPREPLRMVLAADADGRCEIFTWQAATGT
                     ARRVTDSRDGTLHCAIDADARVWWFAEDRNGLGQWYFEEFEGGGPRRPGLTGLRPGLPH
                     GLAVSDAGTVAIGLGDGRGTTVHIGTPGGPARPVATVDGPALLAGISPSGGLLALSGPA
                     GSERAVTLLTRTGAPLDRLSGRHGRLWALGFAPSTAGTELLLVQECGPESTAPHEQRDG
                     RQRTAGDTLNSRYRLASWRPGNGLQPHDWCCFDTEITARWYPEGRRVLIRQDRHGRSTL
                     VTADLDRRTLTPVPTPPGTVLDAAPHPGDDVHYLWTDTATPPRMRSTAGTRLPALATLP
                     GRAPGRHGELWTDGPDGPVHTLLSRPESGSGGCPPLVFLVHGGPADHARDAYDPEVHSL
                     VASGFAVARVNYRGSTGYGPRWRAAFGEGVGLTQVADLVAVRADLLRRGLAREDAIGLW
                     GTSWGGYLTLLALGTRPGLWQAGVAVKPVADCAAAHRSGTPALRALDERLFGGTPETVP
                     ERYARSSPLHYAAAVRAPLLVAAATLDAKCPPGQVRSYLTALREAGVAHESLWLETGHD
                     GYTGAHHMTVLTRAVRFLDRHLRRSGTAHRAPRPSVGGVPGETGTSGAPGSRGPIPDAV
                     HRQNRRSSHAEGHHPQRPARG"
     gene            5001..6935
                     /locus_tag="SL103_RS19820"
                     /old_locus_tag="SL103_19820"
     CDS             5001..6935
                     /GO_function="GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: TIGR03975"
                     /gene_functions="other (smcogs) SMCOG1277: Radical SAM
                     domain protein"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006606751.1"
                     /locus_tag="SL103_RS19820"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SL103_19820"
                     /product="RiPP maturation radical SAM C-methyltransferase"
                     /protein_id="WP_069570310.1"
                     /sec_met_domain="TIGR03975 (E-value: 1.6e-181, bitscore:
                     603.4, seeds: 14, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRVLLVNMPWSPIDLPSLALGILRRSVDERTSGRADVLHANLEFT
                     DWITRRTAFTGDDYQFYALSSYFMGCGDWVFSSALYDDPEWRVPEFNASMRGKLKDARL
                     RMSHELHRVVPEFVQEIAERIVAAGPDVVGFTSTFQQNTAALAAAKYVKRLAPHIRTVM
                     GGANCDAEQGAATHRNFPFVDFVVRGEGEAAFPQLLTALDEDTELSAVPGLCHRGADGS
                     SIANPMSTSPLPPVAILPPDYGGYFERLASSVARNWVEPKLVVEGARGCWWGEKHHCTF
                     CGLNGSFMQFRSKSPDTFYKEIMDLARRHRVLDMYVVDNILDMGYLHSVLPRIIDSGYD
                     LRMHIEIKANMRRPQLRTLAEAGMIYVQPGIESLNSRVLDLMDKGVSGCQNVRMLRDGA
                     ETGLSVSWNYLHGFPGETAADYEPVIAQIPALEHLNPPVDLSARIAIERFSPYFNRPEL
                     GFTGLRPEEHYRFTYDLPESELLDLAYVFEAPERGIGEPTVTALNDALGAWKKRHADSR
                     LTHADLGDRIVLVSRRHAFDWNVMELTRPTDIETFRLLDQPHTPAALTRKLAARLPEHA
                     LDAAAVHVLLQHWVTLGLVFTDNGQYVHVAPAAVNEDLLRLDFMRHTHAAAAPADQPDA
                     ADRPVAAHA"
     gene            6966..7643
                     /locus_tag="SL103_RS19825"
                     /old_locus_tag="SL103_19825"
     CDS             6966..7643
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018088744.1"
                     /locus_tag="SL103_RS19825"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SL103_19825"
                     /product="DUF5825 family protein"
                     /protein_id="WP_069570311.1"
                     /transl_table=11
                     /translation="MPPTTTAPARGALTLSAWRDYDEDACALPGMGLGAVELTGPPEKY
                     ATRLWELGARRVRLTDPIDLTAVGDPDAAAHAVRRLCLVRDLTAHAVVVEWQLRLPPTP
                     DDGWRDLSHLQPPLTLAGPADPAAALTRWRTEHYLCKCLWRQGPGFVQIRDRRWGELRR
                     FTADEPAYHEAIGRLAYGAPPSAVPEAIAADFLQERLIARTGPLLWWLPYRVHRWIQEA
                     MAI"
     gene            complement(7689..8435)
                     /locus_tag="SL103_RS19830"
                     /old_locus_tag="SL103_19830"
     CDS             complement(7689..8435)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1260: AraC family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010033424.1"
                     /locus_tag="SL103_RS19830"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SL103_19830"
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="WP_069570312.1"
                     /transl_table=11
                     /translation="MPNIRHTPAAPAHILALAAGERVDAHRHDNHQIVYAGSGVLAVTT
                     DAGTWFAPGNRAIWVPAGTVHAHRAHGHLELHTLGLPVGDNPLRLDAPTVLAVSPLLRE
                     LILAYTRDPTGTGPARRRLRAVLMDQLRASPHEPVQLPTATDPRLAAVCALLHTDPADP
                     GTLAEFGARTHTGERTLSRLFRSEFGMTFPQWRTQLRLYHALKMLADDLPVTVVAHRCG
                     WSSTSAFIDVFRRAYGYTPGTHNRRA"
     misc_feature    8439..8455
                     /note="TFBS match to HexR, Glucose-responsive regulator,
                     confidence: weak, score: 16.61"
                     /tool="antismash"
     misc_feature    8482..8497
                     /note="TFBS match to CopR, Copper-responsive regulator,
                     confidence: weak, score: 18.27"
                     /tool="antismash"
     gene            8490..9773
                     /locus_tag="SL103_RS19835"
                     /old_locus_tag="SL103_19835"
     CDS             8490..9773
                     /GO_function="GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1106: major
                     facilitator transporter"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012380533.1"
                     /locus_tag="SL103_RS19835"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SL103_19835"
                     /product="MFS transporter"
                     /protein_id="WP_079145869.1"
                     /transl_table=11
                     /translation="MSTTRPLLLLALGHACVDVYQGAVAALVPFFLAERAYTYAAASGI
                     VLAASLLSSVAQPLFGALTDKWPMPWLLPVSTLAGGVGVALSGVGGSYALTLAVVAVSG
                     IGVAAYHPESARIARVASRGSHTAMGWFSLGGNLGFATAPLAVSAVVATGGLRASPLLI
                     VPALVGAALCVAALRAVNPPAPTGRTAPADARPAGRDDWPAFLRMSGAIVCRSIVFVGL
                     SAFISLYARQRSGGGEVAGTVALFVLYLGGAIGTVAGGRLATRYGRVQVVRWSYALTVP
                     AVAGIVLVPGPLLYLCVALTSAGLYVPFSLHVTLGQDYLPRRVGTASGVTLGLTVSVGG
                     IASPLIGAAADATSLQTALAPLIALPALGWLLLRTLHEPAMPEAAVSEAAVSAAAAPEA
                     GAPEAGAPAPATAVARAPHGDTARTTGP"
     gene            complement(9873..10802)
                     /locus_tag="SL103_RS19840"
                     /old_locus_tag="SL103_19840"
     CDS             complement(9873..10802)
                     /GO_function="GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA]"
                     /GO_process="GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /GO_process="GO:0019748 - secondary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003953105.1"
                     /locus_tag="SL103_RS19840"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SL103_19840"
                     /product="aminoglycoside phosphotransferase family protein"
                     /protein_id="WP_069570313.1"
                     /transl_table=11
                     /translation="MKTPTFIDVPDAFAVSYGGLGAEARAWLDGLPRLGGDLLERWDLR
                     PDGPSAHGMASLVLPVLRADGTPAALKLQQLREETAGAATGLRTWRGNGIVRLLDHDEE
                     SSSQLLERLDAGRPLSTVADDGRALQILAELMARLTSVPAPEGLRTLSGIAAAMLDQVP
                     RAVPALHDPAERRLVRSCASAVAELAGEPGDRLLHWDLHYDNVLAGEREPWLAIDPEPL
                     AGDPGFELLPALHNRWAEVVASGDVARAVLRRFDLLTEVLGLDRRRATGWTLGRVLQNA
                     LWDIEDGNTQLEPAQIAIAQALLGRSAR"
     misc_feature    complement(11575..11590)
                     /note="TFBS match to RutR, Regulator of the pyrimidine and
                     purine metabolism, confidence: medium, score: 21.9"
                     /tool="antismash"
     misc_feature    complement(11799..11810)
                     /note="TFBS match to AfsR, Pleiotropic regulatory for
                     antibiotic production, confidence: medium, score: 18.46"
                     /tool="antismash"
ORIGIN
        1 tgttcaaccc gctcaacgac gatcacatcc ccgcggccgt ggcgcagtgc gagtccgccg
       61 aggacgtgcg cgagggagtg ctggcggccg cgaaccgggt gccgctcgcc gcccgcagcg
      121 gcgggcactc gtacgtcggc tactcggcgc cgaaccgcgg cctggtcctc gacctgcgcc
      181 gcatgtcggc ggtgacggtc cagccggacg gcaccgcgag cgtcggcgcg ggcgcctgcc
      241 tgagggacgt ctacagcgga ctcggccagg cgaaccggtg cctgcccggc ggttcctgct
      301 tcaccgtcgg catttccggc gtgacgctgg gcggcggaat cggcgtcctg cagcggaagt
      361 tcgggctgac ctgtgatcat ctggtgtccg ccgacgtcgt caccgccgac gggcggctgc
      421 tcacggtgtc cgcctcacgg acccccgatc tgttctgggc gctgcggggc ggcgggggcg
      481 gcaatttcgg catcgtcacg cggttcacct tcgcaaccga tccggccccg acgctgaccg
      541 tgttcgtgat ggtcttcccg gcggggtcgg cggccaaggt gctgggcgcg tggcagccgt
      601 ggatcaccgc tgcccccaag gagctgtggg ccaacttcaa catgtccggt ggcgacccgc
      661 cgagctgtcg ggtggccggc tgcttcgtcg ggcccggcag tgcgtgtcag cccctgctcg
      721 acgacctgat cgcgcagtcc ggggtcgtcc ccgaccggcg cacggtcgac gagcgtgact
      781 acttcagggc catgcgcttc ttctccggca aaccggaccg cgagtcgttc gtcgcgtcgt
      841 cgcgggtcct gagcaagccc gcccccgacc cgggcgcggt ggtcgccgtg ctcaccggcc
      901 gcacgggcat gtccctcatc gtcgacgcgc tcggcggcgc ggtgaccgac gtaggcgtgc
      961 agcagacggc ctttccgcac cgcaccgcct ttgccaccgc acagatctac gcgaccgcgt
     1021 cggccgcgtc cgaggccgag gtgacccgca gcgtcgccga agtcgtggcc gggctcggga
     1081 agttgggaat gagcggcggc tacgtgaact acatcgaccc ggcgctgccc gaatggccga
     1141 ggtcctacta cggcccgaat ctggaacgtc tgcgttcggt cgcgcacgac tacgacccga
     1201 accgggtctt cgacttcgcg cagagcctgt cgagggcgta ggggccccgt ttcggcccct
     1261 cgtcagcccg acgtagcacc gtcgtgagcg ccggcgaccg caacggtcgt gccttccgcg
     1321 gacattcacc tccgaacgac ttgtggcctg agtcactccg tggctaagct ccggcacgtt
     1381 tcgggcacat ttcctccgca agttgaccta atcggtgagc cgatcttcac gatctgccca
     1441 catagacgtg aggtggctcg catggaacgg cgatcaacgg cgtccccgtc ggggcactta
     1501 ccgtccggat tgtcctccgg ctcggcggcc gatatgccct cggcctttgg tcgtacccgg
     1561 tgacgtcgtc cgccgcgggg gccgccgcgt ccgccgtacc gctgcccggc gcccgccgcg
     1621 acgtccggct gttctggtgg gccggcacgt gcgatgcgct gggcagtcag gtgtccgggc
     1681 tcgtactgcc gttgctgctg ctgagtctgg gctggtcccc ggccgaggtg ggggtcgtcg
     1741 ccgggctgtc cgcggccgcc acgctgctgc tggggccgct cgccgcggtg cccgccgacc
     1801 gcggggcgcg caagagcgtg atggtgggtg ccgcggtggt gtccgcgctc gcgatggcct
     1861 gcgtcgcggt gaccgtggga cgggaaacgg tgctgccggc cgtgctgctg accgccgtcc
     1921 tcgtcgagcg gtgtgcgacc tcgtgctacg aggcggcctc gctcgggacc gtcgccctgg
     1981 tgagtcctcc cggggagcac cggcgcgtgc tgtcccgatt acaggcggga gagcgcggcg
     2041 cgctggttct cgggccggcg ctgggcggac tgctcttcca caccggccgg tggctgccgt
     2101 tcctcgcgga cgcgctctcg tacgccgtcg cggccggctg tatccgtgcc atgcgcgcgg
     2161 atctcgcccc ggcccgggaa cacgggtcac cggccggcac cccgcgccgc ggaccgcgag
     2221 cactggcggc cgaggccggg gcgggcgtgg cggtgatccg ccgcgatccc ttcctgcggc
     2281 tggcgttcgt ctggctcacg gccgtcaacg ccctgctcgt cgcgctgtat tacacgacgg
     2341 tcttcgcgtt gcagggcggc ggccggggtg cggccgcgct ggggctggtg ctcgcgctcg
     2401 ccggggcggc cggcctcggt ggcgcgctgc tcgcgcccgc actggccggc cggcactccg
     2461 cacggcgcgt actggtgccg gtgtcgtggc tgctggtgcc acccgccgcc gccctggcca
     2521 cggcccggga gggctggcag ttcgggctgc tcttcggcct gctgtgcgtg ctgacgccac
     2581 tggccaccgt ggcgctgcag gcccgggtca tccaggtcac cccgccggcg ctgcaggccc
     2641 gcaccggcac cgtgctggcc acggcggcgg gggcgggggc ggccctggcg ccggccctcg
     2701 ccgggctggc cgccgaccgg gccggcgccg ccgtcacctt caccggctgc gccctgctgc
     2761 tggcggtgct cgcgctgtac gcgacctgca cggcaccggg tctcccccgc gccgacgccg
     2821 agggggccgc atgagcgccg gcgatttccg ggtctaccag ccggcgctgc ccgaggtctg
     2881 cccgcgcgag ccgctgcgga tggtcctggc cgcggacgcg gacggccgct gcgagatctt
     2941 cacctggcag gccgccaccg gcaccgcgcg ccgggtcacc gacagccgcg acggaacgct
     3001 gcactgcgcc atcgacgccg acgcccgggt gtggtggttc gcggaggacc gcaacggcct
     3061 cgggcagtgg tacttcgagg agttcgaggg cggcgggccc cggcgtcccg gcctgaccgg
     3121 tctgcgcccg ggcctccccc acgggctcgc cgtcagcgac gccgggacgg tggccatcgg
     3181 cctcggtgac ggccgcggca cgaccgtcca catcgggaca ccgggcgggc cggcacgacc
     3241 ggtggcgacc gtcgacggcc ctgcgctgct ggcgggcatc tcgccctcgg gcgggctgct
     3301 ggccctgtcc gggccggccg gttccgagcg cgcggtcacc ctgctgaccc gcacgggggc
     3361 acccctcgac cggctctccg gacggcacgg ccggctgtgg gcgctgggct tcgcaccgtc
     3421 gacggcgggc acggaactgc tgctcgtgca ggagtgcggg ccggagtcca cggcgcccca
     3481 cgagcagcgg gatgggcggc agcgcacggc cggcgacacc ctcaacagcc gctaccggct
     3541 ggccagttgg cggcccggaa acggtctgca accgcacgac tggtgctgct tcgacaccga
     3601 gatcaccgct cgctggtatc ccgaggggcg gcgggtcctc atccgtcagg accggcacgg
     3661 gcgctccacg ctcgtcaccg ccgacctcga ccggcgcacc ctgaccccgg tgcccacgcc
     3721 gccgggcacc gtgctcgacg ccgccccgca cccgggcgac gacgtgcact acctctggac
     3781 cgacaccgcc accccgcccc ggatgcgctc caccgccggc acccggctgc cggccctcgc
     3841 cacgctgccc ggccgcgcgc ccggccggca cggcgagctg tggaccgacg gcccggacgg
     3901 gcccgtgcac accctgctca gccggccgga gtccgggtcc ggcgggtgtc ccccgctggt
     3961 gttcctcgtc cacggcgggc ccgccgacca cgcccgcgac gcctacgacc cggaggtgca
     4021 ctccctggtc gcgtccggat tcgcggtcgc gcgggtcaac taccgcggtt cgaccggcta
     4081 cggcccccgc tggcgggccg cgttcggcga gggcgtcggg ctgacccagg tcgccgacct
     4141 cgtcgcggtc cgtgccgatc tgctgcgccg cggcctggcc cgcgaggacg ccatcggtct
     4201 gtggggcacc tcctggggcg gctacctgac cctgctggcg ctcggcaccc ggcccggcct
     4261 gtggcaggcg ggcgtcgcgg tcaaaccggt cgcggactgc gcggcggcgc accgcagcgg
     4321 cacccccgca ctgcgggccc tggacgagcg gctgttcggc ggcacgcccg agacggtgcc
     4381 cgaacgctac gcgcggagct cgccactcca ctacgccgcc gcggtacgcg ccccgctgct
     4441 ggtcgccgcc gccaccctcg acgccaaatg cccgccggga caggtacgca gctatctcac
     4501 cgccctgcgg gaggccggcg tcgcgcacga gtcgctgtgg ctggagaccg gccacgacgg
     4561 ctacaccggc gcccaccaca tgaccgtact gacccgcgcc gtgcggttcc tggaccggca
     4621 cctgcgccgc tccggcacgg cccaccgagc tccccgcccc tccgtgggcg gggtccccgg
     4681 ggaaaccggc acgtccggtg cccccggatc gcgaggcccg attcccgatg ccgtacaccg
     4741 gcagaacagg agaagcagcc atgcagaagg acatcatcca caacgacccg ctcgcgggtg
     4801 acgaggagaa ccgcaagccg ggcatcggca tcacggtgac cgtgccgttc cgcaccgcgg
     4861 aggacaccga ggaggagtga ccctccccgg ccggcccggt acgccaccgg gccggccggc
     4921 cgcccgtcgt cacccgcccg acgcccgcgg ggacgcgtgg cggccgcccc ttgccatccg
     4981 ccgccgtacg aggagccgtc atgcgcgttc tgctggtcaa catgccctgg tccccgatcg
     5041 atctgccgtc cctcgcactc ggcatcctcc ggcggagcgt cgacgagcgc acctccggcc
     5101 gggccgacgt gctgcacgcc aatctggagt tcaccgactg gatcacccgg cgcaccgcgt
     5161 tcaccggtga cgactaccag ttctacgcgc tgtcctcgta cttcatgggg tgcggcgact
     5221 gggtgttctc ctccgccctc tacgacgatc ccgagtggcg cgtaccggag ttcaacgcgt
     5281 cgatgcgcgg caagctcaag gacgcgcggc tgcggatgtc gcacgaactg caccgggtgg
     5341 taccggagtt cgtgcaggag atcgccgagc ggatcgtcgc ggccggtccc gacgtcgtcg
     5401 gcttcacctc caccttccag cagaacaccg ccgcgctcgc cgccgccaag tacgtcaaac
     5461 gcctcgcccc gcacatccgg acggtcatgg gcggggcgaa ctgcgacgcc gagcagggcg
     5521 ccgcgacgca ccgcaacttc cccttcgtgg acttcgtggt ccgtggcgag ggcgaggccg
     5581 ccttcccgca gctgctgacc gcgctcgacg aggacaccga gctgtccgcc gtccccggtc
     5641 tgtgccaccg cggcgcggac ggatcgagca tcgccaaccc gatgagcacc agcccgctgc
     5701 cgcccgtcgc catcctgccg cccgactacg gcggctactt cgagcggctg gcgtcgtccg
     5761 tcgcccgcaa ctgggtggag cccaagctcg tcgtcgaggg cgcgcgcggc tgctggtggg
     5821 gggagaagca ccactgcacg ttctgcggcc tcaacggctc cttcatgcag ttccgcagca
     5881 agagccccga caccttctac aaggagatca tggacctggc acgccgccac cgcgtgctgg
     5941 acatgtacgt cgtcgacaac atcctcgaca tgggctatct gcactccgtg ctgccccgca
     6001 tcatcgacag cggctacgac ctgcggatgc acatcgagat caaggcgaac atgcgccggc
     6061 cccagctgcg caccctcgcc gaggccggga tgatctacgt ccagccgggc atcgagagcc
     6121 tgaacagccg ggtgctcgac ctgatggaca agggcgtcag cggctgccag aacgtccgca
     6181 tgctcaggga cggagccgag accgggctgt ccgtctcctg gaactacctg cacggcttcc
     6241 ccggggagac cgccgccgac tacgagccgg tcatcgcgca gataccggcg ctggaacacc
     6301 tcaacccgcc ggtcgacctg tcggcccgca tcgcgatcga gcgtttcagt ccgtacttca
     6361 accggcccga gctcgggttc accggcctgc gccccgagga gcactaccgc ttcacctacg
     6421 acctgccgga gtccgaactg ctggacctgg cctacgtctt cgaggctccc gagcgcggca
     6481 tcggcgagcc gaccgtcacc gccctcaacg acgccctcgg cgcgtggaag aaacgccatg
     6541 cggacagcag gctcacgcac gccgacctcg gcgaccgcat cgtcctggtc agccggcggc
     6601 acgccttcga ctggaacgtc atggaactca cccgccccac ggacatcgag accttccggc
     6661 tcctcgacca gccgcacacc cccgccgcgc tcacccgcaa actcgccgcg cggctgccgg
     6721 agcacgccct cgacgccgcc gccgtccacg tcctcctcca gcactgggtg acgctcggcc
     6781 tggtcttcac cgacaacggc cagtacgtcc atgtggcgcc cgccgccgtc aacgaggacc
     6841 tgctgcgcct cgacttcatg cgccacaccc acgcggcggc tgcgccggcg gaccagccgg
     6901 acgcggcgga ccgccccgtc gcagcccacg cctgagcacc cacccggccc ggagaggaga
     6961 cccccatgcc ccctaccacc accgcccccg cccgaggcgc gctcacgctc agcgcatggc
     7021 gcgactacga cgaggacgcc tgcgcgctgc ccggcatggg cctgggcgcg gtcgaactga
     7081 ccggcccgcc ggagaagtac gccacccggc tgtgggaact cggggcgcgc cgcgtccggc
     7141 tcaccgaccc gatcgacctc accgcggtgg gcgacccgga cgccgccgcg cacgccgtcc
     7201 ggcgcctgtg cctcgtacgc gatctgaccg cgcacgccgt cgtggtggag tggcagctgc
     7261 ggctgccgcc gacgccagac gacggctggc gcgacctcag ccacctgcag ccgccgctga
     7321 cgctggccgg ccccgccgac ccggcggccg ccctgacgcg gtggcgcacc gagcactacc
     7381 tctgcaagtg cctgtggcgc cagggacccg gcttcgtgca gatccgcgac cgccgctggg
     7441 gcgagctgcg ccgcttcacc gcggacgagc cggcctacca cgaggcgatc ggccggctgg
     7501 cgtacggcgc cccgccgtcg gccgtgccgg aggcgatcgc cgccgacttc ctgcaggagc
     7561 ggctgatcgc ccgtaccggc ccgctgctgt ggtggctgcc gtaccgagtg catcgctgga
     7621 tccaggaggc gatggccatc tgaccgcgcg cgggtcacac cgccgggcgc cggcccggcg
     7681 gccggccgct atgcgcggcg gttgtgggtg ccgggcgtgt agccgtacgc gcggcggaag
     7741 acgtcgatga aggcgctggt ggaggaccag ccgcagcggt gggcgaccac ggtgacgggg
     7801 aggtcgtcgg ccagcatctt cagggcgtgg tacaggcgca gttgggtgcg ccactgggga
     7861 aacgtcatgc cgaattcgga gcggaagagg cggctgaggg tgcgctcccc ggtgtgcgtg
     7921 cgggcgccga actcggcgag cgtgccgggg tcggcggggt cggtgtgcag gagcgcgcag
     7981 acggcggcga gccgcgggtc ggtcgccgtg ggcagctgta cgggctcgtg cggtgacgcg
     8041 cggagctggt ccatgagcac cgcgcgcagc cggcggcgtg cggggcccgt gccggtgggg
     8101 tcgcgggtgt aggcgaggat cagctcgcgc agcagggggc tgacggcgag gacggtgggc
     8161 gcgtcgagac gcagcgggtt gtcgccgacg ggcaggccga gggtgtgcag ttcgaggtgg
     8221 ccgtgggcgc ggtgggcgtg gacggttccg gcgggtaccc agatggcccg gttcccgggc
     8281 gcgaaccacg tcccggcgtc ggtggtgacg gccagcacgc cggaacccgc gtagacgatc
     8341 tggtggttgt cgtggcggtg cgcgtcgacg cgctctccgg cggccagagc gaggatgtgg
     8401 gccggcgccg cgggggtgtg gcggatgttc ggcatacctt ggcattttat cggaagcacg
     8461 acagccgcgg cggccgtgac gatcgagaca tgtcaacgac tcgacctctt ctgctgctgg
     8521 ccctcgggca cgcctgtgtg gacgtctacc agggcgcggt ggccgccctc gtgccgttct
     8581 tcctcgccga gcgggcctac acctacgccg cggcgtccgg catcgtcctc gccgcctcgc
     8641 tgctctcctc ggtggcccag ccgctgttcg gcgcgctcac cgacaagtgg ccgatgccgt
     8701 ggttgctgcc ggtgagcacg ctggccggag gcgtcggtgt cgcgctcagc ggcgtcgggg
     8761 gctcgtacgc gctgacgctg gcggtggtcg cggtgtccgg gatcggggtg gccgcgtacc
     8821 atcccgagtc cgcccggatc gcccgtgtcg cgagccgtgg cagtcacacc gcgatgggct
     8881 ggttctccct cgggggcaac ctcggcttcg ccaccgctcc gctcgccgtc tccgccgtcg
     8941 tcgccaccgg cggcctgcgc gcctcaccgc tgctgatcgt cccggccctg gtgggcgccg
     9001 cgctgtgcgt ggccgccctg cgtgcggtca acccgcccgc cccgaccgga cgcaccgcgc
     9061 ccgccgacgc ccgcccggcg gggagggacg actggccggc cttcctcagg atgtccgggg
     9121 ccatcgtgtg ccgctcgatc gtcttcgtcg gtctgagcgc cttcatctcc ctctacgcgc
     9181 ggcagcgcag cggaggaggc gaagtcgcgg gcaccgtggc cctgttcgtg ctctacctcg
     9241 gcggtgcgat cggcaccgtc gcgggcggcc ggctcgcgac gcgttacggc cgcgtccagg
     9301 tggtgcgctg gtcgtacgcg ctgaccgtgc ccgccgtcgc cggcatcgtg ctggtccccg
     9361 ggccgctgct gtacctgtgt gtcgcgctga cctccgccgg gctgtacgtg cccttctccc
     9421 tgcacgtcac cctcggccag gattacctgc cccggcgcgt cggcaccgcc agcggcgtca
     9481 cgttgggtct gacggtcagc gtcggtggca tcgccagccc cctcatcggc gccgcggccg
     9541 acgcgacctc cctgcagacc gccctcgccc cgctcatcgc gctgcccgcg ctgggctggc
     9601 tgctgttgcg caccctgcac gagcccgcca tgcccgaggc cgcggtgtcc gaggccgcgg
     9661 tgtccgcagc cgccgcgccg gaagccggtg cgccggaagc cggtgcgcct gccccggcca
     9721 cggccgtcgc ccgcgcgccg cacggcgaca ccgcgcgcac caccggcccc tgacgcccgt
     9781 gcccccggtg ccgcggccgg caggcccgag ggcatcgggg ccccgggggc ccgggggcag
     9841 cgggaggccc gggactcgcc gggcgaagcg cctcagcgcg ccgaacgacc caacagggcc
     9901 tgggcgatgg cgatctgcgc gggttcgagt tgcgtattgc cgtcctcgat gtcccacagc
     9961 gcgttctgca gcacgcggcc gagcgtccag ccggtggccc gccgccggtc caggccgagg
    10021 acctcggtca gcaggtcgaa gcggcgcagc accgcccgcg cgacgtcgcc cgacgccacc
    10081 acctccgccc accggttgtg cagtgccggc aggagttcga acccggggtc cccggccagc
    10141 ggctcggggt cgatggccag ccagggctcc cgctcgccgg cgaggacgtt gtcgtagtgc
    10201 aggtcccagt gcagcagccg gtctccgggt tcaccggcca gctcggcgac ggcggacgcg
    10261 cagctgcgca ccagtcgccg ttcggcggga tcgtgcagcg ccggcacggc ccgcgggacc
    10321 tggtcgagca tggcggcggc gatgccggac agcgtgcgca gcccctcggg ggcgggcacc
    10381 gaggtcaggc gggccatcag ctcggcgagg atctgcaggg cccggccgtc gtcggcgacc
    10441 gtggacaggg ggcggccggc gtcgagccgt tccagcagct ggctgctgga ttcctcgtcg
    10501 tggtcgagca ggcggacgat gccgttgccc cgccatgtcc gcagtccggt ggccgcgccc
    10561 gcggtctcct cccggagctg ctggagcttg agggcggccg gggtgccgtc ggcgcggagc
    10621 acgggcagga cgagcgaggc catgccgtgt gcggagggac cgtcgggccg caggtcccag
    10681 cgctccagga ggtcgccgcc gaggcgcggc agcccgtcga gccaggcgcg tgcctcggcg
    10741 cccagcccgc cgtaggacac ggcgaacgcg tcgggtacgt cgataaaggt cggcgtcttc
    10801 atacgggcag tctccgtcac cgcccgtacc ggcgtccgag cactcggaca tccgccccgg
    10861 cagacgtacg actccacacc ggcgtcatga gcgatatggc aaggaagatc cacatatgcc
    10921 ttgtctgccg cgtacggacg cggtcaccac tctccgaggt caccgtgtcc acatagcgtt
    10981 cagctcccgg gccgctccgc gtcggtttgt attctcggca ggcgcccccc ttcgacgcgg
    11041 cacctgcgag aggaagcacc atgaccaccg agctgagccc cgacgccctg accgaggtcc
    11101 ttgcgcgggc ccgccaagac taccaggacc tcacggcccg cgggctctcc ctgaacctca
    11161 cccggggcaa gccggcgccg gagcagctcg acctctccga ggaattgctg aacctgcccc
    11221 tcggagtgca cacctccgcc gacggcacgg acgtacgcaa ctacggcggt ctgcagggac
    11281 tgcccgagct gcgggagata ttcgccgagg tgctccaggt gccggccgga cagctgctcg
    11341 ccgccgggaa ctccagcctt gagctgatgc acgactgcct ggtgcacgcc ctgctcggtg
    11401 tcctgccggg cgccacctcg cgctgggtgg agcaggagcg gatcgcgttc ctgtgtccgg
    11461 ttcctggtta cgaccgacac ttcgcgctct gcgagcggtt cgggatcgac atgatcccgg
    11521 tgccgatgac cgccgagggt ccggacatgg acgtcgtgga gcagctggtc gccgaggacc
    11581 cgacggtcaa gggcatctgg tgcgtcccga agtacagcaa cccggacggc gcctgctaca
    11641 gcgacgcgac ggtggcgcgc ctcgcctcga tgaacaccgc ggcccccgac ttccggatct
    11701 tctgggacaa cgcctacgcc acccaccacc tcaccgaaga gcccgtcgag atcgccgacc
    11761 tgctcgccgc ctgcgacgaa gcggggaacc cggaccgggc gttcgtgttc ggctcctcct
    11821 cgaagatcac gatggcgggc gcgggtgtcg ccttcttcgg ctcctcgacc gccaacctga
    11881 agtggctgct cggccacaac gccaagcggt cgatcggccc cgacaaggtc aacca
//
