LOCUS       NZ_CP017157            12886 bp    DNA     linear   CON 31-MAY-2024
DEFINITION  Streptomyces lydicus strain 103 chromosome, complete genome.
ACCESSION   NZ_CP017157
VERSION     NZ_CP017157.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces lydicus
  ORGANISM  Streptomyces lydicus
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 8201357)
  AUTHORS   Jia,N., Ding,M.-Z., Gao,F. and Yuan,Y.-J.
  TITLE     Complete genome sequencing of Streptomyces lydicus 103 and metabolic
            pathways analysis of antibiotic biosynthesis
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8201357)
  AUTHORS   Jia,N., Ding,M.-Z., Gao,F. and Yuan,Y.-J.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2016) Key Laboratory of Systems Bioengineering
            (Ministry of Education), Tianjin University, Tianjin 300072, PR
            China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SMRT Analysis v. 2.3.0
            Genome Coverage                   :: 100x
            Sequencing Technology             :: PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_001729485.1-RS_2024_05_31
            Annotation Date                   :: 05/31/2024 02:23:15
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,288
            CDSs (total)                      :: 7,195
            Genes (coding)                    :: 7,061
            CDSs (with protein)               :: 7,061
            Genes (RNA)                       :: 93
            rRNAs                             :: 7, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 47 of 134
            Pseudo Genes (incomplete)         :: 104 of 134
            Pseudo Genes (internal stop)      :: 11 of 134
            Pseudo Genes (multiple problems)  :: 26 of 134
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 01:52:31
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 3479832
            Orig. end                         :: 3492718
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP017157.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Source DNA is available from Prof. Ying-Jin Yuan.
            Annotation modified by submitter.
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..12886
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="hydrogen-cyanide"
                     /region_number="10"
                     /rules="(Fer2_4 and cds(Pyr_redox_2 and Fer2_BFD) and DAO)"
                     /tool="antismash"
     cand_cluster    1..12886
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(Fer2_4 and cds(Pyr_redox_2 and Fer2_BFD)
                     and DAO)"
                     /kind="single"
                     /product="hydrogen-cyanide"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..12886
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:7886](+)"
                     /cutoff="5000"
                     /detection_rule="(Fer2_4 and cds(Pyr_redox_2 and Fer2_BFD)
                     and DAO)"
                     /neighbourhood="5000"
                     /product="hydrogen-cyanide"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..7886
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="5000"
                     /detection_rule="(Fer2_4 and cds(Pyr_redox_2 and Fer2_BFD)
                     and DAO)"
                     /neighbourhood="5000"
                     /product="hydrogen-cyanide"
                     /protocluster_number="1"
     misc_feature    complement(71..86)
                     /note="TFBS match to ClgR, Caseinolytic protease gene
                     regulator, confidence: weak, score: 20.96"
                     /tool="antismash"
     misc_feature    complement(134..149)
                     /note="TFBS match to CRP, Cyclic AMP receptor protein,
                     confidence: medium, score: 17.13"
                     /tool="antismash"
     gene            223..1092
                     /locus_tag="SL103_RS15055"
                     /old_locus_tag="SL103_15055"
     CDS             223..1092
                     /GO_function="GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /GO_function="GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003958579.1"
                     /locus_tag="SL103_RS15055"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SL103_15055"
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="WP_069569436.1"
                     /transl_table=11
                     /translation="MAIVLGKPGVDGLSEAVGVLREWQYDGAPMQLHPGDLGWFWRSGA
                     EATAAAVRTWRRDGRILAVGLLDGPGLLRLTIAPDAQREEELARQVVADVTRPERGVLP
                     GGRVNIEAPMGALVQDLLSEAGWKTDEPWTPLRHGLAEPLADPGVRIEVVGPQRAHVFV
                     AVHRAAFEGSRFTAERWHAMAAGLPYADARSLVAYDDRGDAVAAVTVWSAGPGRPGLLE
                     PMGVHREHRRHGYGEAITRAAAAALRDLGSSSALVCTPSSNAAAVATYRSAGFRPRPEV
                     RDRYRDAS"
     gene            1186..1683
                     /locus_tag="SL103_RS15060"
                     /old_locus_tag="SL103_15060"
     CDS             1186..1683
                     /GO_function="GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1111: GCN5-related N-acetyltransferase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018093558.1"
                     /locus_tag="SL103_RS15060"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SL103_15060"
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="WP_069569438.1"
                     /transl_table=11
                     /translation="MRFVELSSKTMIALLQDDLARASAEAGVALSEYFVTDTAKWVWRY
                     RLDQLTSDPGCAGWLTQAVVAEPEGAVVGYAGFHGPPDEVGMVELGYSVVPVHRRQGYA
                     RAMLAALVQRAVAEARVRTVRVTISPDNTASLATISGCGFVEVGEQWDEEDGLELIFEM
                     PV"
     misc_feature    complement(1739..1753)
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 14.7"
                     /tool="antismash"
     gene            1836..2384
                     /locus_tag="SL103_RS15065"
                     /old_locus_tag="SL103_15065"
     CDS             1836..2384
                     /EC_number="2.-.-.-"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1111: GCN5-related N-acetyltransferase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014671332.1"
                     /locus_tag="SL103_RS15065"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SL103_15065"
                     /product="GNAT family protein"
                     /protein_id="WP_069569440.1"
                     /transl_table=11
                     /translation="MPELKRLHADHAPAVLAFELANRAYFAASVPDRGDEFFARFADRY
                     HALLAEQEAGICAFYLLVAADGSVLGRFNLVDIEDRAAHLGYRVAQHVTGRGVATASVR
                     ELCRLAAARHGLRTLRAAAARRNAASRKVLTKAGFVPVGPAEPADLGGEPGTWYQRDLV
                     LQPSGAPLPGAGRGPATAI"
     gene            complement(2479..3264)
                     /locus_tag="SL103_RS15070"
                     /old_locus_tag="SL103_15070"
     CDS             complement(2479..3264)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014047570.1"
                     /locus_tag="SL103_RS15070"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SL103_15070"
                     /product="uracil-DNA glycosylase"
                     /protein_id="WP_069569442.1"
                     /transl_table=11
                     /translation="MDETDPAGPPDSADPAFPARQAARCADLAELDARVVRCRACPRLV
                     AWREQVAAQPRAAFRDWEYWARPVPGFGPPDAALAVVGLAPAAHGGNRTGRMFTGDASG
                     DFLFAALHAVGLASQPTATHRGDGLELYGVRVTAPVHCAPPDNKPTPAERRTCRPWLAA
                     ELEFLAPSLRTVVALGAVGWQAALPVLGDAGWPLPRPRPAFGHGAELTLPATGRRRQLH
                     VLGSYHPSQRNTFTGRLTFDMLVDLLTRAARSAGLGSGD"
     gene            3453..3917
                     /locus_tag="SL103_RS15075"
                     /old_locus_tag="SL103_15075"
     CDS             3453..3917
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007443815.1"
                     /locus_tag="SL103_RS15075"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SL103_15075"
                     /product="VOC family protein"
                     /protein_id="WP_069569444.1"
                     /transl_table=11
                     /translation="MTATPLHWKLVVDAHDPHAQADFWAAALGYEVEDHSVLIGKLLGL
                     GAVPPELTVGRHDRRAWRDAAAVRHPDDPYDAESGTGLGRRLLFNRVPQAEKKTAKNRL
                     HIDLHGRAGELDTEVERLRALGARVVDHVKEPSGEWVVMTDPEGNEFCVH"
     gene            complement(3914..4690)
                     /locus_tag="SL103_RS15080"
                     /old_locus_tag="SL103_15080"
     CDS             complement(3914..4690)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014150892.1"
                     /locus_tag="SL103_RS15080"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SL103_15080"
                     /product="pyridoxamine 5'-phosphate oxidase family protein"
                     /protein_id="WP_079145757.1"
                     /transl_table=11
                     /translation="MESAENVHPAHPRRLPQTDRTRHRRLREQGRLDRDALDAVLAAGF
                     VCHLGVTVDGTPLVVPTVYGADGDTLYFHGSVASRSLAASPGAPVCVTVTHVDGLVLAR
                     SVFEHGVNYRSAMVFGTPRPVTDPDEKLAGLRCLTEQAAPGQWDYARPPSRKELAATAL
                     LALSLDEASVKVRTGPPDDGDGPDAALGLWAGELPLHTSWGPLRPDPRLPTGIEPPPHL
                     TARPTATADQGTSGGSPAPSHTAGPDPSGPCATPAP"
     misc_feature    4672..4686
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 14.07"
                     /tool="antismash"
     misc_feature    4713..4727
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: weak, score: 16.48"
                     /tool="antismash"
     misc_feature    4979..4988
                     /note="TFBS match to ToxT, Regulator of MSHA biosynthesis,
                     confidence: weak, score: 13.04"
                     /tool="antismash"
     gene            5001..6170
                     /locus_tag="SL103_RS15085"
                     /old_locus_tag="SL103_15085"
     CDS             5001..6170
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     hydrogen-cyanide: DAO"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1103: FAD dependent oxidoreductase"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016642316.1"
                     /locus_tag="SL103_RS15085"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SL103_15085"
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="WP_069569446.1"
                     /sec_met_domain="DAO (E-value: 8.4e-67, bitscore: 224.9,
                     seeds: 521, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MPTRYGHHLDTSDVVIVGAGAVGAACAYYAARSGLSVTVVDRGAV
                     AGGTTGAGEGNLLVSDKEPGPELDLALLSARLWRELADALPPEIEYETKGGLVVAGDEP
                     GLAALRTLSGAQAAAGVASSYLPAGRLQDAEPHLAPGLAGGVLYPQDAQVQPALAAAHL
                     LRVSGARLHLGAEVTAVLRGPGGAVRGVRTPRGDLLAPAVVNATGTWAGDLAGLAGVTV
                     PVLPRRGYVLVTEPLPRIVRRKVYAADYVADVASDSAALQTSAVVEGTPAGPVLIGASR
                     ERVGFDRTLSVEVIRRLAARAAALFPVLAGVRVMRAYQGFRPYLPDHLPAIGPDPRAPG
                     LLHACGHEGAGIGLAPATGRLIAAALTATPAPLDTAPFRPDRFVERSPA"
     gene            6167..6493
                     /locus_tag="SL103_RS15090"
                     /old_locus_tag="SL103_15090"
     CDS             6167..6493
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     hydrogen-cyanide: Fer2_4"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005309276.1"
                     /locus_tag="SL103_RS15090"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SL103_15090"
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="WP_069569448.1"
                     /sec_met_domain="Fer2_4 (E-value: 1.7e-24, bitscore: 84.4,
                     seeds: 114, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSRRRRRTPAALVRARPEPAFEISLDGRPLPALPGQSLAAALWAA
                     GVLSWRRTRGSGRPRGAFCGIGSCHDCLATVNGTPNQRACLVPARPGDAVTTQEGHGRA
                     GLAV"
     gene            6474..7886
                     /locus_tag="SL103_RS15095"
                     /old_locus_tag="SL103_15095"
     CDS             6474..7886
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     hydrogen-cyanide: Fer2_BFD"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     hydrogen-cyanide: Pyr_redox_2"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1176: alkyl hydroperoxide reductase subunit"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006606671.1"
                     /locus_tag="SL103_RS15095"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SL103_15095"
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="WP_069569450.1"
                     /sec_met_domain="Pyr_redox_2 (E-value: 1.8e-26, bitscore:
                     91.8, seeds: 64, tool: rule-based-clusters)"
                     /sec_met_domain="Fer2_BFD (E-value: 6.9e-10, bitscore:
                     37.9, seeds: 211, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MPASPSDPRGSVHDLAVIGAGPAGLAAAVTAADLGLTVALLDAAP
                     RPGGQFYRQPAPGLGAARPQALHHAWGAFTRRAARLAAHRATGRVHHLAEHHVWAVTRA
                     TADPEHWAVHAVTGPDGAHGPAPELTARHVLLATGAYERQLPFPGWTLPGVVGAAGAQA
                     MLKAGLVLPGRRIVVAGSGPLLLAVAGSLAAAGARVPVVAEAGDYRAYARSPRALAAQP
                     RKLVEAAGHAATLARHGVRLRTRSAVTAVHGTDRVEVVTLTRLDRDWRPLPGTGVRIPC
                     DALAVGHGLVPQLEPATGLGCATRPTPDGGHALVVDGRQRTTVAGIWAAGETCGVAGAE
                     LAFIEGELAARSIAGRGPVAALRRRRDRLRRFALTMAAVHAPRPGWSDWLRTDTEVCRC
                     EEVTAGRIQDAVTGLGARDGRTVKLLTRAGMGWCQGRMCGTAVTHLAGDTDPAPTRRPL
                     ACPVFLDHLTDG"
     gene            7920..8819
                     /locus_tag="SL103_RS15100"
                     /old_locus_tag="SL103_15100"
     CDS             7920..8819
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /GO_process="GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA]"
                     /GO_process="GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006606672.1"
                     /locus_tag="SL103_RS15100"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SL103_15100"
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="WP_069569452.1"
                     /transl_table=11
                     /translation="MTTRTRPWHGIMVATTLPLRDDLSVDHDAYAAQVARLLAAGCDGV
                     VPNGSLGEYQTLTEDERIRVVQTAVAAAGGDGDRVMPGVSAYGSAESRRWAERAAEAGA
                     GSVLLLPPNGYRAGPAAVRAHFAEVAAAGVPVVAYNNPFDTKVDLTPQLLGALHADGSI
                     VAVKEFSGDVRRAYEIAEVAPDLDLLIGADDVLLELALAGAVGWIAGYPNALPASCAAL
                     YRAATAHDLDTALPLYRALHPLLRWDSKPGFVQAIKFSMDLAGHHGGPTRPPRLPLADE
                     ELGVLRAATEKLLADGHH"
     gene            8833..9834
                     /locus_tag="SL103_RS15105"
                     /old_locus_tag="SL103_15105"
     CDS             8833..9834
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019891275.1"
                     /locus_tag="SL103_RS15105"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SL103_15105"
                     /product="proline racemase family protein"
                     /protein_id="WP_069569453.1"
                     /transl_table=11
                     /translation="MRTRHVFHAVDSHTEGMPTRVITGGVGTIPGATMADRRRHFAEHR
                     DDLRTLLMYEPRGHAAMSGAILQPPTRPDADYGVLFIEVSGFLPMCGHGTIGVATVLVE
                     TGMVEVREPVTTVRLDTPAGLVTAEVRVRDGAATAVTLTNVPAFCAALDRKITVPGYGS
                     IGYDLAYGGNFYAVVPLDSVGLPFDRARKDDILAAGLALMDAVNATDRPVHPEDDRIGG
                     LKHVYFAAPGSDATRSRHAMAIHPGWFDRSPCGTGTSARMAQLHARGELPLHQDFVNES
                     FIGTEFTGRLVGETTVGGLPAVVPTVTGRAWLTGTAQYFLDPADPFPAGFRL"
     misc_feature    complement(9879..9889)
                     /note="TFBS match to HypR, L-hydroxyproline utilization
                     repressor, confidence: weak, score: 20.42"
                     /tool="antismash"
     gene            9938..10654
                     /locus_tag="SL103_RS15110"
                     /old_locus_tag="SL103_15110"
     CDS             9938..10654
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1071: GntR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014057181.1"
                     /locus_tag="SL103_RS15110"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SL103_15110"
                     /product="GntR family transcriptional regulator"
                     /protein_id="WP_069569455.1"
                     /transl_table=11
                     /translation="MGELKHRSLITAQERLRDQVAHQLRAALIAGELRPGAVYSAPGLA
                     ADFGVSATPVREAMLDLAREGLVEPVRNKGFRITEVSERDLDQYTEIRALIEVPVVGRI
                     TRTATREQLEALRPVALEIVTAARAHDLIGYLEADRRFHLELLGLSGNDRLVETVGELR
                     KRSRLYGLTRLDEHDQLLPSAEEHLELLDVMLTGDAAAAEACMARHLGHVRSLWAQGRE
                     ETPAPPAQRRPAARQG"
     misc_feature    10778..10794
                     /note="TFBS match to ZuR_variant_2, Zinc-responsive
                     repressor, confidence: weak, score: 15.29"
                     /tool="antismash"
     misc_feature    10782..10792
                     /note="TFBS match to HypR, L-hydroxyproline utilization
                     repressor, confidence: weak, score: 18.94"
                     /tool="antismash"
     misc_feature    11559..11572
                     /note="TFBS match to ANR, Anaerobic transcriptional
                     regulator, confidence: weak, score: 18.18"
                     /tool="antismash"
     misc_feature    complement(11596..11611)
                     /note="TFBS match to ClgR, Caseinolytic protease gene
                     regulator, confidence: weak, score: 20.91"
                     /tool="antismash"
ORIGIN
        1 ccttccgcag ctccgggcgg tcctgctcgg aaagggcgat ggacatggtg gtccgctcct
       61 tcacctctgt tgttctcggt cggcgtcgtg ccgctgcgac gagcttccgc cctgccccga
      121 ggtcaacctc aactgtgatc cggctcacca ctgcgtccga ggtcaggtgc cggcccttga
      181 accgggtgcg gaccgtcggc gcggtggtgt gtgatcgggc gcatggcgat cgtgttgggc
      241 aagccgggag ttgacgggct gagcgaggcg gtgggcgtac tgcgggagtg gcagtacgac
      301 ggggcgccga tgcagctgca tccgggggac ctgggctggt tctggcggtc gggtgcggag
      361 gcgaccgccg cggcggtcag gacgtggcgc cgggacggac ggattctcgc cgtcgggctg
      421 ctggacggcc ccgggctgtt gcggctgacg atcgcgccgg acgcccagcg ggaggaggag
      481 ttggcgcggc aggtggttgc cgacgtgacc cggccggagc gcggcgtgct gcccggggga
      541 agggtgaaca tcgaggcgcc gatgggtgcg ctcgtccagg atctgctgtc cgaggccggc
      601 tggaagaccg acgagccgtg gacgccgctg cgccacggcc tcgcggagcc gttggcggac
      661 ccgggcgtgc ggatcgaggt ggtcgggccg cagcgggcgc acgtgttcgt cgccgtgcac
      721 cgggcggcgt tcgaggggtc gaggttcacg gccgagcgct ggcacgcgat ggcggccgga
      781 ctgccgtacg ccgatgcccg gagtctggtg gcgtacgacg accggggcga cgcggtggcg
      841 gcggtgacgg tgtggtcggc cggcccgggc aggcccgggt tgctcgaacc gatgggcgtg
      901 caccgggaac accgccggca cggctacggc gaggcgatca cccgcgccgc ggcggccgca
      961 ctccgggacc tgggctcgtc gagcgcgctc gtctgcaccc cgagctccaa cgccgccgcc
     1021 gtcgccacct accggtcggc cggcttccga ccacgccccg aggtacggga ccggtaccgg
     1081 gacgcgtcgt aaaaccgttg gacacgtcct ggacgcgcca cgacgattcc cgctgtgaca
     1141 gacagagccg agtgccggac cccggactca acgagcggga agtccgtgcg attcgttgag
     1201 ttgtcgagca agaccatgat cgccttgctg caggacgacc tggcgcgagc cagtgccgaa
     1261 gcaggtgtcg ctctcagcga gtacttcgtc accgacacgg caaagtgggt gtggcggtat
     1321 cggctcgatc agctgacctc ggaccccggt tgcgcgggtt ggctcacgca ggccgtggtg
     1381 gccgagccgg aaggtgccgt cgtggggtac gccgggttcc atggaccgcc ggacgaggtg
     1441 ggcatggtgg aactcggtta ctcggtggtc cccgtccacc gtcgtcaggg ctacgccaga
     1501 gcgatgctgg ccgcattggt gcagcgagcc gttgccgaag cccgcgtcag gaccgtgcgg
     1561 gtgacgatca gtcctgacaa cacggcttcc ctggccacga tttccggatg tggcttcgtc
     1621 gaggtcggag aacaatggga cgaggaagac ggcctcgaac tcatctttga aatgcctgtc
     1681 tgaccggggg cgagggtccg gacccgcaga gcgcggtcca ctcctacgcc gcgaacagaa
     1741 attgcagaaa ccactctgcc gggcacgggc cgacgagccc cgtcggtggc ggaaaccggt
     1801 ggaggctcgg cgtaccacct gggtaaggtc gggcggtgcc cgagctgaag cggctgcatg
     1861 ccgaccatgc cccggcggtc ctggccttcg aattggcgaa ccgcgcctac ttcgctgcct
     1921 cggtccccga ccgcggcgac gagttcttcg cccggttcgc cgaccggtac cacgccttgc
     1981 tggctgagca ggaggccggc atctgtgcct tctacctgct cgtggccgcg gacggctcgg
     2041 tactcggccg gttcaatctg gtcgacatcg aggaccgtgc tgcgcacctc ggctaccggg
     2101 tggcgcagca cgtcaccggc cgcggcgtgg cgaccgcgag cgtccgggag ctgtgtcggc
     2161 tggcggcggc ccggcacggg ctgcgcacac tgcgggcggc cgccgcccgc cggaacgccg
     2221 cgtcccggaa ggtgctgacc aaagccgggt tcgtccccgt cgggcccgcc gaaccggccg
     2281 acctcggcgg tgagccgggc acctggtatc agcgcgacct ggtgctccaa ccgtccggtg
     2341 cgcccttgcc gggggccggc cgagggccgg cgacggccat ctgagcatcg gtgcgtgagg
     2401 actgacgttc ccgcgtcccg ggcagccggc gaccgggacc acggaggcga cccgagagcg
     2461 cgatctgcgg ccgcagtact agtcgccgct ccccagcccc gcgcttcggg cggcccgggt
     2521 gagcaggtcg acgagcatgt cgaaggtgag gcggccggtg aaggtgttgc gctggctggg
     2581 gtggtagctg cccaggacgt gcagttggcg ccgccggccg gtggcgggca gggtcagttc
     2641 ggcgccgtgg ccgaaggcgg gacggggccg ggggaggggc cacccggcgt cgccgaggac
     2701 gggcagcgcc gcctgccagc cgacggcgcc cagcgccacc accgtgcgca ggctcggcgc
     2761 gaggaactcc agctcggccg ccaaccaggg gcggcaggtg cggcgttcgg cgggtgtcgg
     2821 cttgttgtcc ggcggggcgc agtgcaccgg tgcggtgacc cgcacgccgt acagctccag
     2881 cccgtcgccc cggtgcgtcg cggtcggctg cgaggcgaga cccaccgcgt gcagcgcggc
     2941 gaacaggaag tcacccgagg cgtcgccggt gaacatccgc ccggtgcggt tgccgccgtg
     3001 cgcggcaggt gccagaccga cgacggccag cgccgcgtcc ggcgggccga accccggcac
     3061 cggccgtgcc cagtactccc agtcccggaa cgccgcgcgc ggctgcgcgg cgacctgctc
     3121 gcgccacgcc accagccggg ggcaggcccg gcagcgcacc acccgcgcgt ccagttccgc
     3181 caggtcggcg cagcgggccg cctgacgggc cgggaaggcg gggtccgcac tgtccggcgg
     3241 tccggccggg tccgtctcgt ccatacggcc acgctagacg agaggttccg ccgggtgcgg
     3301 gcacgtccgg cgacacggag cgcgcatccg ggaacgcgtc agggatgtgg ctgcggtcgg
     3361 cgacctgagg cgcaccgccg gcagcacggc accacccgac cgggtgcggg cggcggctcc
     3421 gatgtcggtg ccggcggcta taagtgggga ccatgaccgc aaccccactg cactggaagc
     3481 tcgttgtcga cgcccacgac ccgcacgccc aggccgactt ctgggcggcg gcgctcggct
     3541 acgaggtcga ggaccacagc gtcctcatcg gtaagctcct cggcctcggc gccgtcccgc
     3601 ccgaactcac cgtcggccgt cacgaccggc gcgcctggcg ggacgcggcg gcggtacgcc
     3661 atcccgacga cccgtacgac gcggagagcg gcacgggact ggggcgtcgg ctgctgttca
     3721 accgcgtacc gcaggccgag aagaagacgg cgaagaaccg cctgcacatc gacctgcacg
     3781 gccgcgccgg cgagctggac accgaagtcg agcggctccg cgccctcggc gcccgggtgg
     3841 tggaccacgt caaggaaccg tccggcgagt gggtggtcat gacggatccg gagggcaacg
     3901 agttctgcgt gcactagggc gcgggcgtcg cgcacggacc acttgggtcc ggccccgctg
     3961 tgtgggaggg ggccgggctc cccccggacg tgccctggtc tgccgtggca gtcggacggg
     4021 cggtgagatg gggtgggggc tcgatgcctg tggggaggcg cgggtcgggt cggagggggc
     4081 cccaggaggt gtgcaggggg agttcgccgg cccagaggcc cagcgcggcg tcgggcccgt
     4141 cgccgtcgtc cggcgggccg gtgcgcacct tgaccgacgc ctcgtcgagg gacagggcca
     4201 gcagcgcggt ggcggcgagc tccttgcggc tcggggggcg ggcgtagtcc cactggcccg
     4261 gggcggcctg ttcggtcagg cagcgcagtc cggccagctt ctcgtccggg tcggtgaccg
     4321 gccggggcgt gccgaacacc atcgcgctgc ggtagttgac gccgtgctcg aacacggagc
     4381 gggcgaggac cagcccgtcg acgtgggtga cggtgacgca gacgggcgcg ccgggcgagg
     4441 cggccaggct tcggctggcg accgacccgt ggaagtagag cgtgtcgccg tccgcgccgt
     4501 agaccgtggg gacgacgagc ggggtgccgt ccacggtcac gccgaggtgg cagacgaagc
     4561 cggcggccag tacggcgtcg agggcgtcgc ggtcgagacg gccctgttcg cgcagtcggc
     4621 gatggcgggt gcggtcggtc tgcggcagcc gccgagggtg ggcggggtgc acgttttctg
     4681 ctgattccat gagcgattgt tctaccgtgc ggcgaaattc gttgtccatg gggtttgttg
     4741 cgacgaattc ggttgggtgg ggtgtcgcct ggggggcggt gagttgtgcg tccgggacgt
     4801 ggaggccctg cccctgggcc gccatcggca ctcccgctca agcccaacgg cgcgaccgac
     4861 cgggctgccc tcggcgagct gctcgggggg cccggccgcc cgtcctgacg cgtacgccgc
     4921 gccgcgcgtc cggccgtcgg agccgccgcg ccggatcaac tccccttctc agaaacagtg
     4981 ttgtgtgaca tattactgag gtgcccacga gatacggtca ccacctggac acctccgatg
     5041 tcgtcatcgt cggggccggt gcggtcggcg ccgcctgcgc ctactacgcc gcccggtccg
     5101 gcctctccgt caccgtcgtc gaccgtggcg cggtcgcggg cggcaccacc ggcgccggcg
     5161 agggaaacct gctcgtctcc gacaaggagc cgggcccgga actcgacctc gcgctgctct
     5221 ccgcccggct gtggcgcgaa ctcgccgacg cccttccgcc ggagatcgag tacgagacca
     5281 agggcgggct ggtcgtcgcc ggcgacgagc cgggcctggc cgccctgcgc acgctcagcg
     5341 gggcgcaggc cgcggcgggc gtggcgagca gctacctccc ggccggccgg ctgcaggacg
     5401 ccgagcccca tctcgcgccc ggcctcgccg gcggcgtcct ctacccgcag gacgcacagg
     5461 tccaacccgc cctggccgcc gcgcacctgc tgcgcgtctc aggcgcccgg ctccacctgg
     5521 gcgccgaggt caccgccgtc ctccggggcc ccggcggagc ggtccgcggc gtccgcaccc
     5581 cgcgcggcga cctcctcgcg cccgccgtcg tcaacgccac cggcacctgg gccggcgacc
     5641 tcgccgggct cgccggggtg accgtcccgg tactgccgcg ccgcggatac gtcctggtca
     5701 ccgagccgtt gccgcgcatc gtccggcgca aggtgtacgc cgccgactac gtcgccgacg
     5761 tggccagcga ctcggccgca ctgcagacct ccgccgtcgt cgagggcacc ccggccggcc
     5821 cggtgctcat cggtgccagc cgcgaacgcg tcggcttcga ccgcacactc tcggtcgagg
     5881 tgatccgccg gctcgccgcc cgggccgcgg ccctcttccc cgtgctggcc ggggtccggg
     5941 tgatgcgtgc ctaccagggc ttccgcccct acctccccga ccacctcccc gccatcggcc
     6001 ccgacccgcg cgcccccggc ctgctgcacg cctgcggcca cgagggcgcc ggcatcggcc
     6061 tcgcccccgc gaccggccgg ctgatcgccg ccgcgctgac cgcgaccccc gcacccctgg
     6121 acaccgcgcc cttccggccg gaccgcttcg tggagaggag cccggcatga gccgccgccg
     6181 tcgccgaacc cccgccgccc tcgtacgcgc ccgtccggaa ccggcgttcg agatcagcct
     6241 ggacggcaga cccctgcccg cactgcccgg ccagagcctc gcggccgcgc tctgggcggc
     6301 cggcgtgctc tcctggcggc gcacccgcgg cagcgggcgg ccgcgcggcg ccttctgcgg
     6361 catcggctcc tgccacgact gcctggccac cgtcaacggc acccccaacc aacgggcctg
     6421 cctggtcccg gcccggccgg gggacgccgt caccacccag gagggacacg gccgtgccgg
     6481 cctcgccgtc tgacccccgg ggctccgtcc acgacctcgc cgtcatcggg gcggggccgg
     6541 ccgggctcgc cgccgccgtc accgcggccg acctcggact gaccgtggcc ctgctcgacg
     6601 ccgcaccccg ccccggcggc cagttctacc ggcagccggc acccggcctc ggcgccgccc
     6661 gcccgcaggc cctgcaccac gcctggggcg ccttcacccg ccgcgcggcc cgcctggcgg
     6721 cacaccgcgc caccggtcgg gtccaccacc tcgccgagca ccacgtctgg gccgtcaccc
     6781 gggccaccgc cgacccggaa cactgggccg tccacgcggt gaccggaccc gacggtgcgc
     6841 acggccccgc cccggagctg accgcacgtc atgtgctgct cgccaccggg gcgtacgagc
     6901 gccagctccc ctttcccggc tggacgctgc ccggtgtcgt cggtgcggcc ggcgcgcagg
     6961 cgatgctcaa ggccggtctg gtgctgccgg ggcggcggat cgtggtcgcg ggcagcgggc
     7021 cgctgctgct cgccgtcgcc ggttcgctgg ccgccgccgg agcgcgggtc ccggtcgtcg
     7081 ccgaggcggg cgactaccgg gcgtacgccc gcagtccccg cgccctggcc gcccagcccc
     7141 gcaagctcgt cgaggcggcg ggccacgccg ccaccctggc ccggcacggc gtacgcctgc
     7201 ggacccggag cgcggtcacc gccgtgcacg gcaccgaccg ggtcgaggtc gtcaccctga
     7261 cccggctcga ccgcgactgg cggccgctgc ccggcaccgg cgtacgcatc ccgtgcgacg
     7321 ccctcgcggt gggccacggc ctggtgccgc aactggagcc ggccaccgga ctcggctgcg
     7381 ccacccgccc cacgcccgac ggcggccacg ccctggtcgt cgacgggcgg cagcggacca
     7441 cggtcgcggg gatctgggcg gcgggggaga cctgcggtgt cgccggcgcc gaactcgcct
     7501 tcatcgaagg cgagttggcg gcccgctcga tcgccggccg gggtccggtt gcggcgctgc
     7561 ggcggcgccg ggaccggttg cgccggttcg cgctgaccat ggccgccgtc cacgcgccgc
     7621 gccccggctg gtccgactgg ctgcgcaccg acaccgaggt gtgccgctgc gaggaggtca
     7681 ccgccggccg catccaggac gccgtcaccg gcctcggcgc ccgcgacggc cgcaccgtca
     7741 aactcctcac ccgcgccggc atgggctggt gccagggccg gatgtgcggc accgccgtga
     7801 cacacctcgc gggggacacc gaccccgcgc ccacccgccg tcccctggcc tgcccggtct
     7861 tcctcgacca cctcaccgac ggctgagaca ccttcgtccg caccccaagg agactccgca
     7921 tgaccacccg cacccgcccc tggcacggca tcatggtcgc caccaccctg cccctgcgcg
     7981 acgacctctc cgtcgaccac gacgcctacg cggcccaggt cgcccggctg ctcgcggccg
     8041 gctgcgacgg cgtggtgccc aacgggtcgc tcggcgagta ccagaccctc accgaggacg
     8101 aacgcatccg ggtcgtgcag acggccgtgg cggccgcggg cggcgacggc gaccgggtga
     8161 tgccgggcgt ctcggcctac ggcagcgccg agtcgcgccg ctgggccgag cgggccgccg
     8221 aggccggcgc cggctccgtc ctgctgctgc cgcccaacgg ctaccgtgcc ggcccggccg
     8281 cggtccgcgc ccacttcgcc gaggtcgccg cggccggcgt cccggtcgtc gcctacaaca
     8341 accccttcga caccaaggtc gacctcaccc cgcagctgct cggcgcgctg cacgccgacg
     8401 gcagcatcgt ggccgtcaag gagttcagcg gcgacgtccg cagggcgtac gagatcgccg
     8461 aagtcgcccc ggacctggac ctgttgatcg gggcggacga cgtgctgctg gaactcgccc
     8521 tcgccggggc ggtcggctgg atcgccggct accccaacgc gctgcccgcc tcctgcgccg
     8581 ccctctaccg cgccgcgacc gcccacgacc tcgacaccgc gctgcccctg taccgcgccc
     8641 tgcacccgct gctgcgctgg gactccaagc ccggcttcgt gcaggcgatc aagttctcca
     8701 tggacctcgc cggacaccac ggcggcccca cccggccgcc ccgcctcccg ctcgcggacg
     8761 aggagttggg cgtgctccgc gccgccaccg agaagctgct cgccgacggc caccactgag
     8821 aggagcagcc ccgtgcgcac ccgccacgtc ttccacgccg tcgactccca caccgagggc
     8881 atgcccaccc gggtcatcac cggcggcgtc ggcaccatcc ccggcgccac catggccgac
     8941 cgccggcgcc acttcgccga gcaccgcgac gacctccgca ccctgctgat gtacgagccg
     9001 cgcggccacg ccgccatgag cggcgccatc ctccagccgc ccacccggcc cgacgccgac
     9061 tacggcgtcc tcttcatcga ggtctcgggg ttcctgccga tgtgcgggca cgggacgatc
     9121 ggcgtggcca ccgtcctggt cgagaccggc atggtggagg tgcgcgaacc ggtgacgacc
     9181 gtgcggctcg acacccccgc cggactggtc accgccgagg tgcgggtgcg ggacggcgcg
     9241 gccaccgccg tcaccctcac caacgtcccc gcgttctgcg ccgcactgga ccgcaagatc
     9301 accgtccccg gatacggcag catcggctac gacctcgcct acggcggcaa cttctacgcc
     9361 gtcgtcccgc tcgacagcgt cggactgccc ttcgaccggg cccgcaagga cgacatcctc
     9421 gccgccgggc tggcgctgat ggacgcggtc aacgccaccg accggccggt gcaccccgag
     9481 gacgaccgga tcggcggcct caagcacgtc tacttcgccg cgcccgggtc cgacgccacc
     9541 cgctcccggc acgccatggc catccacccc ggctggttcg accgctcgcc gtgcggcacc
     9601 ggcacctcgg cccggatggc gcagctgcac gcccgcggcg aactgccgct gcaccaggac
     9661 ttcgtcaacg agtcgttcat cgggacggag ttcaccggcc ggctcgtcgg ggagaccacc
     9721 gtcggcgggc tgccggccgt cgtccccacc gtcaccgggc gcgcctggct gaccggcacc
     9781 gcccagtact tcctcgaccc ggccgacccc ttcccggcgg gcttccggct ctgaccgcgc
     9841 ggcggcggca ccgggcactg ccaggcggtt tgcgtaacgt gacattgtac gctggcactc
     9901 cgcgccgact cgcagggcca tacggagggc caccaccatg ggtgaactca agcaccgcag
     9961 cctgatcacc gctcaggagc ggctgcgcga ccaggtcgcc caccagctgc gcgccgccct
    10021 gatcgccggc gaactccggc cgggggccgt ctactcggcg cccggcctcg cggcggactt
    10081 cggcgtctcc gccaccccgg tgcgcgaggc gatgctcgat ctggcccgcg aggggctggt
    10141 cgagccggtg cgcaacaagg gtttccggat caccgaggtg agtgagcgcg acctcgacca
    10201 gtacaccgag atccgtgcgc tgatcgaggt cccggtcgtc ggccggatca cccgcaccgc
    10261 cacccgcgaa cagctggaag cgctccggcc ggtcgcgctg gagatcgtca ccgccgcccg
    10321 cgcccacgac ctcatcgggt acctggaggc cgaccgccgc ttccacctcg aactgctggg
    10381 cctgagcggc aacgaccgcc tcgtcgagac ggtgggcgag ctgcgcaagc ggtcccgcct
    10441 gtacggcctg acccgcctcg acgagcacga ccagctgctg ccgtccgccg aggaacacct
    10501 ggagctgctc gacgtgatgc tgaccgggga cgccgcggcg gccgaggcgt gcatggcccg
    10561 tcacctgggc cacgtccgct ccctgtgggc gcagggccgc gaggagacgc ccgccccgcc
    10621 cgcgcagcgc cggccggcgg cccgtcaggg ctgatcgccc gcacgggggt tgacggggcg
    10681 tctgtgagag tcggtcgccc cgggcggccg tgggcgggct gatccgggtc gggccgtgag
    10741 ggggaaactt tacccgggcc cggttcctga agggttgtca gtgcaatttc acattactat
    10801 gttacgggtc acacctcgtg gagtgatggc ggcacccctc gcccctcacc cacccaaccc
    10861 cccaccccct cctggaggtt caggtgtccc cttcgcgtac ccgacgcaga ctcctgctga
    10921 ccgcgaccct cgtcgccacc ctcgcgatgc cgttcgtcca gaccgccggg gcggcacccc
    10981 gcgggacggc gccggccgcc cccgcccggc agccgcaccc ggcgccccgg accgccgacc
    11041 ggaccgccgg cgatccccac gaccgggccc aacgcctgtc ccgtacaccc cggttcagcg
    11101 ccacacccgc acccgcgccc cgcgccgcca cgaccgacgg ccgcataccg ggcccgcaga
    11161 ccggccccgc cgcccccggc caccgggccg cacccgccgt cgccccctgc accctcgacg
    11221 gggtcaccgg cctgaacccc gagcagttcg ccgacttcct cgccgacccg gccgtcaccg
    11281 ccgacggctg cctgcgcgac ctcatctgga cctgggaccc ccggctggct cccgtcatgt
    11341 ccgacgccca tgtgcaggcc gtcgcccggc gcatcgccgc cctcgccgcc tcccacgacg
    11401 gcaccgacgc cggccacctc tacgagatgt tcacgtacct gcacgcggtg gcctaccagg
    11461 acttctcaca cgacgagatc gacaccaccg acaccccgac cgtcaccgcg gtccgccgcg
    11521 cggtcgacgc cttcggcgcc gccgcccaca ccttcgacgt gacccggacc aacgccgaca
    11581 ccctgcgcga ggccctgtac gccatcagcg ggccgggcgg acgccagtac caactcgggc
    11641 tgatccagcg ggtcctggcc accctcgacg ccgcccaccc cgccacccac aaggacccgg
    11701 cctggggcaa cgccgccctc gccgccctct cggtgaacta cctgggcgtc taccccggca
    11761 acaaggacac cgccttccgg gccgcggtcg tcgccgaccc cgcctaccgc gccgccttca
    11821 aagccttcgc cgattacacc cacctcaagg gcaccgccaa cgcctgggcg gtccgcgacg
    11881 cgctcggtga atacggtcgc ttcggccaga tcgactccct caagccggcg atcgtcaccg
    11941 acctcggggc cctcctgacc acgaccgtgc gcaacttcgg cgagggcagc gacccctggg
    12001 ccaaggtcgt cggctggctg ggcaccttcg acgcctgtaa gccctacggc gtctgcaaga
    12061 ccgacatcga gaagcggatc ttcccgtaca cctactccta cgacaacggc gccatcaagg
    12121 tgcgcaccgc cctcgaccgc gccaccgtcg accagctgta ctacgcgtcc aagcaggtca
    12181 aggcccagtt ccaccgggtg ctcgggacca ccgaaccgct ggccggcgac cccaacacca
    12241 cgctcaccat cgtcctgtac gcctcgcgcg ccgactacga ggtctaccac ccgctgctca
    12301 ccgggatggg caccaacaac ggcggcgtct acatcgagca gggcgccacc ttctacacct
    12361 accagcgccg cgtcccccag gactccagcc tcaccctcga agagctcttc cggcacgagt
    12421 acgtccacta cctcaacggc cggttcgccg tgcccggttc cttcggcgag ggcccctggt
    12481 acgagggcga ccgcaccacc gccatggacg agggcagcgc ggaattcttc gacggctcga
    12541 cccgtgacga cggcatcgcc gtacggcggt cactcgtccg cgacatcatc gcggacaccg
    12601 cgggcggcac accgcgcatg agcatcaacg ccttcctgca cgcgacctac gaccacgacg
    12661 gcttccgctt ctactcctac gccgggacgt tcttcgagtt cctgtggcgc gaacaccccg
    12721 ccaggctgca ggagatgtac cggtacctgc gcgccgacga ccccgcgggc ttcgacgcct
    12781 ggcgcacccg gcagagcacc gacgccgccc tccagcagca gtacgacgcc ttcctcgacg
    12841 cccagatcgc ccacgtcgac gacctcttcg taccgcacac cgcctt
//
