LOCUS       NZ_CP017157            10213 bp    DNA     linear   CON 31-MAY-2024
DEFINITION  Streptomyces lydicus strain 103 chromosome, complete genome.
ACCESSION   NZ_CP017157
VERSION     NZ_CP017157.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces lydicus
  ORGANISM  Streptomyces lydicus
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 8201357)
  AUTHORS   Jia,N., Ding,M.-Z., Gao,F. and Yuan,Y.-J.
  TITLE     Complete genome sequencing of Streptomyces lydicus 103 and metabolic
            pathways analysis of antibiotic biosynthesis
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8201357)
  AUTHORS   Jia,N., Ding,M.-Z., Gao,F. and Yuan,Y.-J.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2016) Key Laboratory of Systems Bioengineering
            (Ministry of Education), Tianjin University, Tianjin 300072, PR
            China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SMRT Analysis v. 2.3.0
            Genome Coverage                   :: 100x
            Sequencing Technology             :: PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_001729485.1-RS_2024_05_31
            Annotation Date                   :: 05/31/2024 02:23:15
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,288
            CDSs (total)                      :: 7,195
            Genes (coding)                    :: 7,061
            CDSs (with protein)               :: 7,061
            Genes (RNA)                       :: 93
            rRNAs                             :: 7, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 47 of 134
            Pseudo Genes (incomplete)         :: 104 of 134
            Pseudo Genes (internal stop)      :: 11 of 134
            Pseudo Genes (multiple problems)  :: 26 of 134
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 01:52:31
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 3367127
            Orig. end                         :: 3377340
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP017157.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Source DNA is available from Prof. Ying-Jin Yuan.
            Annotation modified by submitter.
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10213
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="9"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10213
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10213
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:5213](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5213
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            840..1748
                     /locus_tag="SL103_RS14530"
                     /old_locus_tag="SL103_14530"
     CDS             840..1748
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1014: LysR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018091736.1"
                     /locus_tag="SL103_RS14530"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SL103_14530"
                     /product="LysR family transcriptional regulator"
                     /protein_id="WP_069569275.1"
                     /transl_table=11
                     /translation="MIEARRLHILRAVADHRTVTAAAAALYLTPSAVSQQLAALEQETG
                     HRLVDRSARGVRLTAAGDILLTHANAVLAQLERAESELAAYGSGEAGTVTVAAFATGIG
                     LVVAPALTALARSAPGIRVHVQDAEGDASLRMVLDRQVDVAVAVEYRGAPRADDPRLTR
                     VPLYAEPFDAVLPTGHRLAEAPRVAVAALADDPWIGPYPGNPCHDVVVLACENAGFQPR
                     LEHTSDDFRAVVALASAAAGVALVPRSALRDVDLTGVVVRPVDGVAPTRRVFAAVRRGA
                     EDHPLFRPVLAALREAAAPQG"
     misc_feature    859..875
                     /note="TFBS match to NtrC, Nitrogen regulatory protein C,
                     confidence: weak, score: 21.67"
                     /tool="antismash"
     gene            1843..3195
                     /locus_tag="SL103_RS14535"
                     /old_locus_tag="SL103_14535"
     CDS             1843..3195
                     /GO_function="GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA]"
                     /GO_process="GO:0006821 - chloride transport [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018091737.1"
                     /locus_tag="SL103_RS14535"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SL103_14535"
                     /product="chloride channel protein"
                     /protein_id="WP_069569277.1"
                     /transl_table=11
                     /translation="MAVGPTGPGGGTAPGVPQDPYAPARTRGYLKLLLIAAVIGVPVCA
                     AAFGFLALVHALEPLIVEALPRALGFAGTPWWWPVPMLVVAGLLVGLTVRHLPGHGGHE
                     PADGLALGGAPGITALPGIVLAALVSLSFGVVLGPEAPLIALGGGLAAGAVRLVGRGLS
                     ERSVAVVGAAGSFAAVSALLGSPLLGAFLLMEVAAPVGPLLGALLLPGLLASGVGALIF
                     TGLGNWTGLGTYSLALHDVPGAAQPTVTEFGWALVIGLAAACFAGGVRRLSLLLKERVG
                     RHRVPMTTAMGLVVGALAAGFALATGKPATEMLYSGQSALGSLFQDSTGYSVGALLLLL
                     VCKGLAYCASLSAFRGGPIFPALFLGAAGGVALSHLPGLSLTAAFAMGVGALGVAMLRL
                     PMTSVLLATLLMGSNGLTVMPLVIVAVVVAHVAALRLTPAPAPAAPAPSAG"
     gene            complement(3208..4239)
                     /locus_tag="SL103_RS14540"
                     /old_locus_tag="SL103_14540"
     CDS             complement(3208..4239)
                     /GO_function="GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018091739.1"
                     /locus_tag="SL103_RS14540"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SL103_14540"
                     /product="PHP domain-containing protein"
                     /protein_id="WP_099055417.1"
                     /transl_table=11
                     /translation="MEPVEALERIAFLLERQGAETYRVQAFRTAAAAIRALSDDELRGR
                     AEDGTLSRLKGLGPKTTRVVEEAFAGVRPVYLARLEDEAGGPLLDGEQGQRLRRALRGD
                     CHTHSDWSDGGSPVEEMARTARDLGHAWCVLTDHSPRLTVARGLSAERLREQLALVAEV
                     NEALAPFRLLTGIECDILDDGTLDQEPELLDRLDVVVASVHSKLRMDRGPMTRRMLAAV
                     RNPLVDVLGHCTGRLLTRKPRPESQFDADEVFAACAEHRTAVEINCRPERLDPPRRLLR
                     RALDAGCLFSIDTDAHAPGQLDWQLYGCARAEECGVPAERVINTWTERQLATWTRTGRT
                     PHG"
     misc_feature    complement(4422..4437)
                     /note="TFBS match to FuR_variant_1, Ferric Uptake
                     Regulator, confidence: weak, score: 17.27"
                     /tool="antismash"
     misc_feature    4425..4440
                     /note="TFBS match to FuR_variant_1, Ferric Uptake
                     Regulator, confidence: weak, score: 17.12"
                     /tool="antismash"
     misc_feature    complement(4606..4615)
                     /note="TFBS match to ToxT, Regulator of MSHA biosynthesis,
                     confidence: weak, score: 15.17"
                     /tool="antismash"
     misc_feature    4779..4792
                     /note="TFBS match to ArgR, Regulator of arginine
                     biosynthesis genes, confidence: weak, score: 16.87"
                     /tool="antismash"
     gene            5001..5213
                     /locus_tag="SL103_RS14545"
                     /old_locus_tag="SL103_14545"
     CDS             5001..5213
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: strepbact"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018849411.1"
                     /locus_tag="SL103_RS14545"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SL103_14545"
                     /product="hypothetical protein"
                     /protein_id="WP_033270189.1"
                     /sec_met_domain="strepbact (E-value: 1.1e-23, bitscore:
                     82.2, seeds: 15, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MREIQTQEIADSELDNVAGGLVGNLAGTATSTVDGATGLNVGGTL
                     GTVTGTVEGLTGVNTGQVTGLVAGL"
     gene            5415..6221
                     /locus_tag="SL103_RS14550"
                     /old_locus_tag="SL103_14550"
     CDS             5415..6221
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007386622.1"
                     /locus_tag="SL103_RS14550"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SL103_14550"
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="WP_069569281.1"
                     /transl_table=11
                     /translation="MQFRQQALSKLQSPEELDLPVRFARPQGWLVLIITVAVLIGASFW
                     AVTGTVSSTLNAPGILTHGQGSYVLQSPVAGQVTAVLAQEGRRVAANTPLLRVRTDRGT
                     TVVRSLAAGRLTTMVATIGSVVTTGADVASVERVAHRDDPLTATLYVPADSGANVPVGG
                     SVDLTVQSVPSQQYGVLRGRVKAVGRTAQTRQRIGAYLGSSQLGEQFSKSGPPVAVLVQ
                     LDPAPRNRSGYAWSTSGGPPYAVDSMTLASGAVHLAAQHPIDWLLP"
     misc_feature    complement(5426..5443)
                     /note="TFBS match to ArcA, Repressor of carbon oxidation,
                     confidence: weak, score: 14.48"
                     /tool="antismash"
     gene            6257..8506
                     /locus_tag="SL103_RS14555"
                     /old_locus_tag="SL103_14555"
     CDS             6257..8506
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /GO_function="GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /GO_process="GO:0006508 - proteolysis [Evidence IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Peptidase_C39"
                     /gene_functions="transport (smcogs) SMCOG1288: ABC
                     transporter related protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005320917.1"
                     /locus_tag="SL103_RS14555"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SL103_14555"
                     /product="NHLP family bacteriocin export ABC transporter
                     peptidase/permease/ATPase subunit"
                     /protein_id="WP_079146235.1"
                     /sec_met_domain="Peptidase_C39 (E-value: 4.4e-38, bitscore:
                     128.8, seeds: 36, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MPPPGRGRHRPEPAPGARRTRRAAPPAPKAKKARTVRTPTVLQME
                     AVECGAASLAMVLAHYGRHVPLEELRIACGVSRDGSRASNLLKAARSYGLQAKGMQMEP
                     AALAEVQAPAILFWEFNHYVVYDGTGRRFGRRGVYINDPDKGRRFVPTEDFDTSFTGVA
                     LVFEPSESFRKGGRKPGVLSAVPARMRGTTGTLLAALLASLLLVAVGAAVPALSRTYID
                     MFLIGNQTSLLGPLFASMAAMVALTAVLTGLQQANLLRGRIISSTLTSARFLRHLLRLP
                     VTFFAQRSPADLVQRLQSNDAVAETLARDLAAAGVDGIVVVLYAFLLWSYDPQLTVIGV
                     GIALLNIVAMRIVVRLRATHTAKLRADSARLTNTAYTGLQLIETMKATGGENGYFRRWA
                     GQHATTLEVQQRLGVPSAWLAVVAPTLATLNSALILWIGGMRAVEGHISIGLLVAFQAL
                     VTRFTAPLTRLNGVAGRIQDFAADVARLKDVESFPADSLYARPGSDADTRRLKGHVTLE
                     DITFGYSPLDKPLLSGFSLTVGPGRQVALVGGSGSGKSTVSRLISGLYSPWEGTIRIDG
                     RRLEDLSRSALAASVSFVDQDVFLFEGTVRDNVALWDPSIPDDAVIAALRDAALYDDVI
                     SRRPDGIHSRVEQDGRNFSGGQRQRLEIARALVRRPSILVLDEVTSALDAQTEQIIMDN
                     LRRRGCACVVIAHRLSTVRDSDEIVVLDHGVVVERGRHEDLVAAGGTYADLVKEH"
     misc_feature    complement(9911..9920)
                     /note="TFBS match to AbrC3, Antibiotic production
                     activator, confidence: strong, score: 18.16"
                     /tool="antismash"
ORIGIN
        1 tccatcatcg acggcatccg gctgtccaag gcccgccgct tccgctacgc caaccgcgac
       61 atggccgacc tggaacgcca gttgaaggag gccgccgaag ggggcgccgg ccgcacgctg
      121 atcgtcaccg acggcgtctt ctcgatggac ggctacctgg cgcccctgga cgagatctgc
      181 gatctggccg accgccacgg cgccatggtg atggtcgacg actcgcacgc ggtcggcttc
      241 gtcggccccg gcggccgcgg cacccccgag ctgcacggcg tcatggaccg cgtcgacatc
      301 gtcaccggca ccctcggcaa ggccctcggc ggcgcctccg gcggctatgt cgcggcccgc
      361 gcggagatcg tcgccctgct gcgccagcgc tcgcgtccgt acctcttctc caactcgctc
      421 gccccggtga tcgccgccgc ctcgctgaag gtcctcgacc tgctggagtc ggccggtgag
      481 ctgcgcgagc ggctgcacgc caacaccgcg ctgttccgct cccggatgac cgcggagggc
      541 ttcgacgtcc tcccgggcga ccacgccatc gcgcccgtca tgatcggcga cgcggccgag
      601 gcggcccgca tggcggagct gctcctggag cgcggcgtgt acgtcatcgg cttctcctac
      661 ccggtcgtcc cgcagggcca ggcgcggatc cgcgtccagc tgtcggccgc acactccacc
      721 gaggacgtca accgcgcggt ggacgccttc gtggccgcgc ggaccgccat ggcggccgag
      781 gcgggggccg ccgccgcggg gtgacccgac cagcgcggcc ggcctgagaa aatagccaca
      841 tgatcgaagc acgccggctg cacatcctcc gtgcggtggc cgaccaccgc acggtcaccg
      901 ccgcggccgc cgcgctctac ctcacgccgt cggcggtctc ccagcagctg gcggcgctgg
      961 agcaggagac cgggcaccgc ctcgtcgacc ggagcgcccg cggcgtgcgg ctgaccgccg
     1021 cgggcgacat cctgctcacc cacgccaacg ccgtcctcgc ccagctggaa cgcgccgagt
     1081 ccgagctcgc cgcgtacggc tcgggcgagg ccggcacggt caccgtcgcg gccttcgcca
     1141 ccggcatcgg cctggtcgtc gcccccgcgc tcaccgcgct cgcccgctcc gcgcccggca
     1201 tccgggtcca cgtccaggac gccgagggcg acgccagcct gcggatggtc ctcgaccggc
     1261 aggtcgatgt ggcggtggcc gtcgagtacc gcggcgcccc gcgcgcggac gacccccggc
     1321 tgacccgcgt cccgctctac gcggagccgt tcgacgcggt gctgcccacc ggccaccggc
     1381 tcgccgaggc gccccgggta gcggtcgccg cgctcgccga cgacccctgg atcggcccct
     1441 accccggcaa cccctgccat gacgtggtcg tcctggcctg cgagaacgcc ggcttccagc
     1501 cccggctgga gcacacctcc gacgacttcc gcgcggtcgt cgccctcgcc tcggccgcgg
     1561 cgggcgtggc gctcgtgccg cgctcggcgc tgcgcgacgt ggacctcacg ggggtggtgg
     1621 tccggcccgt cgacggggtc gcgcccaccc gccgggtgtt cgccgcggta cgccgcgggg
     1681 ccgaggacca cccgctgttc cgcccggtgc tggccgcgct gcgggaggcg gcggcaccgc
     1741 aggggtgacg cgacggcgtt cgggtggcgc ggaccgcgtc ctgcgcgccg ggcgggcccg
     1801 gccgtggcag gctcctcgtg gcggcggcga ggaggcgaga cgatggcggt cggccccacc
     1861 ggcccgggcg gcgggacagc gcccggtgtg ccccaggacc cctacgcccc ggcccgcacc
     1921 cgcggctatc tgaagctgct gctgatcgcg gcggtgatcg gggtcccggt ctgcgccgcc
     1981 gcgttcggct ttctggcgct ggtgcacgca ctggaaccgc tgatcgtgga agcgctgccg
     2041 cgggcgctgg gcttcgccgg gacgccgtgg tggtggccgg tgccgatgct ggtggtggcc
     2101 gggctgctgg tcgggctgac ggtccgccac ctgccgggcc acggcggcca cgagccggcc
     2161 gacgggctgg cgctcggcgg cgcacccgga atcaccgcgc tccccggcat cgtgctcgcg
     2221 gccctggtct ccctcagctt cggggtggtc ctcgggccgg aggcgccgct gatcgccctc
     2281 ggcggcgggc tggccgcggg cgccgtacgg ctggtgggcc ggggcctgtc ggagcggagc
     2341 gtcgccgtgg tgggcgccgc cggcagcttc gcggcggtca gcgcgctgct cggctccccg
     2401 ctgctgggcg cgttcctgct gatggaggtg gcggcgccgg tcggtccgct gctgggcgcg
     2461 ctgctgctgc ccggcctgct cgcgtccggc gtgggcgcgc tgatcttcac cgggctgggg
     2521 aactggaccg ggctcggtac gtactcgctc gcgctgcacg acgtccccgg cgccgcccag
     2581 ccgaccgtca cggagttcgg ttgggcgctg gtcatcggac tggccgcggc ctgcttcgcg
     2641 ggcggcgtac ggcggctctc gctgctgctg aaggagcggg tcgggcggca ccgggtgccg
     2701 atgacgacgg cgatgggcct ggtggtcggg gcgctggcgg ccgggttcgc gctggccacc
     2761 ggcaaacccg cgaccgagat gctctactcg ggacagtcgg cgctcgggtc gctgttccag
     2821 gacagcaccg gctactcggt gggggcgctg ctgctcctcc tggtgtgcaa gggactggcc
     2881 tactgtgcgt cgctcagcgc cttccggggc ggcccgatct tcccggcgct gttcctcggc
     2941 gcggccggcg gggtcgccct gtcccacctg cccggactgt cgctcaccgc ggccttcgcg
     3001 atgggcgtcg gcgcgctggg ggtggcgatg ctccggctgc cgatgacctc ggtgctgctg
     3061 gccacgctgc tgatgggcag caacggactg accgtcatgc cgctggtcat cgtcgccgtg
     3121 gtggtggcgc acgtcgccgc gctgcggctc accccggcgc cggccccggc cgcccccgcg
     3181 ccgtccgccg gctgacccct tccgggttca gccgtgcggg gtgcggcccg tacgggtcca
     3241 ggtggccaac tggcgctccg tccaggtgtt gatgacccgc tccgccggga cgccgcactc
     3301 ctcggcgcgg gcgcagccgt agagctgcca gtccagttgc cccggtgcat gggcgtcggt
     3361 gtcgatggag aacaggcagc ccgcgtccag ggcacggcgc agcagccggc gcggggggtc
     3421 caggcgctcc gggcggcagt tgatctccac cgcggtgcgg tgctccgcgc aggccgcgaa
     3481 cacctcgtcg gcgtcgaact gcgactcggg gcgcggtttg cgggtgagca gccgcccggt
     3541 gcagtgcccg agcacgtcca ccagcgggtt gcgcaccgcg gcgagcatcc gccgcgtcat
     3601 cgggcccctg tccatccgca gtttggagtg caccgaggcc accaccacgt ccagccggtc
     3661 cagcagttcc ggctcctggt cgagcgtgcc gtcgtcgagg atgtcgcact cgatcccggt
     3721 caacagccgg aacggcgcca gcgcttcgtt cacctcggcg accagcgcca gctgctcccg
     3781 cagccgctcg gcggacagcc cgcgggcgac ggtgaggcgg ggggagtggt cggtcagcac
     3841 acaccaggcg tggccgaggt cgcgggcggt ccgggccatc tcctcgaccg ggctgccgcc
     3901 gtccgaccag tccgagtgcg tgtggcagtc cccgcgcagc gcccgccgca gccgctgccc
     3961 ctgttctccg tccagcagcg gcccgcccgc ctcgtcctcc aggcgcgcca gatacaccgg
     4021 gcgcactccc gcgaacgcct cttcgaccac ccgggtggtt ttggggccga gccccttgag
     4081 gcggctgagc gtgccgtcct cggcgcgccc gcgcagctcg tcgtcggaca gcgcccggat
     4141 cgccgcggcg gccgtacgga acgcctgtac gcgataggtc tccgccccct gccgctccag
     4201 caggaaggcg atccgctcca gcgcctcgac gggctccacg tgcggctcct tcccggtgcg
     4261 ccggcccagg tccccttcac ggtgccccac ccgggggccg gctgccaccg ggggtggtac
     4321 cggcggtaaa tcgcccattt cagccgagtc ggaagccgtg ccgttatgcg gttatgtccg
     4381 ttctcgggtc ggtcgatccg gttgcttcgg aaaggcgtac ggttagtgct gatcattccc
     4441 tgcaaggtgc gcaaggtggg caataccgtc ccggtaactt cgggtgaatc gggcgcggtt
     4501 aaaactcccg caaagctcac gcaacattcg gcgaagggtg ccgggaggcg cgtgtgccga
     4561 cctcccggcc gacgggtgaa ccggtgccgt aaaaattgac ggccttcaaa aaatctccac
     4621 atcgaatgcg cagtcagggc acggttgtcg gagtggcttc cccctgctga cctgcgcctc
     4681 attggattgg tgcgggaatg tccagttcgc cgaacgcagt cggagtgctt ctgcggaagt
     4741 gaaaccgaag gagttgtgaa gaagctgcca ctgattcatg gatgtgcgtg cacgacacgt
     4801 aattcaatgg tgcgatgaca cccggatcgg cggttgtccg attagcaggg caacgggcct
     4861 gcatgctttg atccttcgca ccgcggaagt cgcactgagg ttctcgattt tgaggccccg
     4921 gcgaccgctg ccgcggccgg accgccctgc cgggcgactc ggtcgcagct atttgagata
     4981 tgcatccgaa aggaaacacc atgcgcgaga ttcagaccca ggaaatcgcc gacagcgagc
     5041 tcgacaacgt ggccggtggc ctcgtcggca acctggccgg caccgccacc tccaccgttg
     5101 acggcgccac gggcctcaac gtcggcggca ccctgggcac cgtcaccggc accgtcgagg
     5161 gtctgaccgg cgtcaacacc ggccaggtca cgggcctcgt cgccggtctc tgagacccac
     5221 ggcacgtcgg cggcccctcc ttgccgacat gccggccggc tgccccggaa ccgcctggcc
     5281 ttcgccgggt tccggggcac ccggctgccg tgccgcggac ggacccccgt acgctcggct
     5341 gcaccgcggt gcccggggcg cgccgccccg tccgtggtgt cctgacagat cgttgcagtt
     5401 gagggaagag tgtcgtgcag ttccgtcaac aggccctttc caagctgcag tcaccggagg
     5461 agttggatct tccggtgcgc ttcgcccgcc cccagggctg gctcgtactg atcatcacgg
     5521 tcgccgtgct gatcggtgcc tccttctggg cggtgaccgg caccgtctcc tccacgctca
     5581 acgcccccgg catcctcacc cacggccagg gcagctacgt cctgcagagc cccgtcgcgg
     5641 gccaggtcac cgccgtcctc gcccaggagg gccggcgggt ggccgccaac accccgctgc
     5701 tcagggtccg gaccgaccgg ggcaccaccg tcgtgcgcag cctcgccgcc ggccggctga
     5761 ccaccatggt cgccaccatc ggctccgtcg tcacgaccgg cgcggacgtc gcctccgtcg
     5821 agcgcgtcgc gcaccgcgac gacccgctga ccgcgacgct gtacgtcccc gccgacagcg
     5881 gtgccaacgt ccccgtgggc ggctcggtcg acctcaccgt gcagtccgtg cccagtcagc
     5941 agtacggcgt gctgcgcggg cgggtgaagg ccgtcggccg caccgcccag acccgccagc
     6001 ggatcggcgc ctacctgggc agcagccagc tcggtgagca gttctccaag tccggaccgc
     6061 ccgtcgcggt cctggtacag ctcgacccgg cgccgcggaa caggtccggc tacgcctggt
     6121 cgacctccgg cggcccgccc tacgcggtcg actccatgac gctggccagc ggcgccgtcc
     6181 acctggccgc gcagcacccg atcgattggc tgctgccgtg accgcaccga acgactccgc
     6241 ggccccgcag gcgcagctgc cgccgccggg ccgcggccgc caccgccccg agcccgcgcc
     6301 cggcgcccgc cgcacccgcc gcgccgcgcc gcccgccccc aaggccaaga aggccaggac
     6361 cgtccgcacc cccaccgtcc tccagatgga ggcggtggag tgcggcgccg cctcgctggc
     6421 catggtcctc gcccactacg ggcgccatgt gccgctggag gagctgcgga tcgcctgcgg
     6481 cgtctcccgc gacggctcgc gcgccagcaa cctgctcaag gccgcccgca gttacgggct
     6541 ccaggccaag ggcatgcaga tggaaccggc cgcgctcgcc gaggtgcagg cccccgccat
     6601 cctcttctgg gagttcaacc actacgtcgt ctacgacggc acgggccgcc gcttcggccg
     6661 ccgcggcgtg tacatcaacg accccgacaa gggccgccgc ttcgtcccga cggaggactt
     6721 cgacaccagc ttcaccggtg tcgcgctggt cttcgagccc tccgagagct tccgcaaggg
     6781 cggccgcaag cccggcgtgc tgagcgcggt gcccgcccgg atgcgcggca ccaccggcac
     6841 cctgctcgcc gccctgctcg ccagcctgct gctggtcgcg gtcggcgccg cggtgcccgc
     6901 gctgagccgt acgtacatcg acatgttcct gatcggcaac cagacgtcgc tgctggggcc
     6961 gctcttcgcc tcgatggccg cgatggtcgc gctgaccgcc gtactgaccg ggctgcaaca
     7021 ggccaatctg ctgcgcggcc ggatcatctc ctccaccctg accagcgccc gcttcctgcg
     7081 ccacctgctc agactcccgg tcaccttctt cgcccagcgc agcccggccg acctcgtgca
     7141 gcggctgcag tccaacgacg cggtcgccga aaccctggcc cgcgacctgg ccgccgcggg
     7201 cgtcgacggc atcgtcgtcg tcctctacgc cttcctgctg tggagctacg acccccaact
     7261 gaccgtcatc ggtgtgggca tcgcgctgct gaacatcgtc gcgatgcgga tcgtggtgcg
     7321 gctgcgcgcc acccacacgg cgaagctgcg ggcggacagc gcccggctga ccaacaccgc
     7381 ctacaccggc ctccagctca tcgagacgat gaaggccacc ggcggggaga acggctactt
     7441 ccgccgctgg gccggccagc acgccaccac gctggaggta cagcagcggc tcggcgtccc
     7501 gagtgcctgg ctggccgtgg tcgcccccac cctcgccacc ctcaacagcg cgctgatcct
     7561 gtggatcggc ggtatgcggg cggtcgaggg ccacatctcc atcggtctgc tggtcgcctt
     7621 ccaggcactg gtgacccgct tcaccgcgcc gctgacccgg ctcaacggcg tcgccggccg
     7681 gatccaggac ttcgccgccg atgtggcgcg cctcaaggac gtcgagtcct tccccgccga
     7741 cagcctctac gcgcgccccg gctcggacgc cgacacccgc cggctcaagg gccacgtgac
     7801 gctggaggac atcaccttcg gctacagccc cctggacaaa ccgttgctca gcggcttctc
     7861 gctgaccgtc ggccccggcc ggcaggtggc cctcgtcggc ggctccggca gcggcaagtc
     7921 caccgtctcg cggttgatat ccgggctcta cagcccctgg gaaggcacca tccgcatcga
     7981 cggccggcgc ctggaggacc tctcccgcag cgcgctcgcc gcctcggtgt ccttcgtcga
     8041 ccaggacgtc ttcctcttcg agggcaccgt ccgcgacaac gtggcgctgt gggacccctc
     8101 gatccccgac gacgcggtca tcgccgccct gagggacgcc gcgctctacg acgacgtcat
     8161 atcccggcgc cccgacggca tccacagccg cgtcgagcag gacggccgca acttctccgg
     8221 cgggcagcgg cagcgcttgg agatcgcccg cgccctggtc cggcgcccca gcatcctggt
     8281 cctcgacgag gtcaccagcg ccctggacgc ccagaccgaa cagatcatca tggacaacct
     8341 gcggcggcgc ggctgtgcct gcgtcgtcat cgcccaccgg ctgagcaccg tccgcgacag
     8401 cgacgagatc gtggtcctcg accacggcgt ggtcgtcgaa cgaggccggc acgaggacct
     8461 ggtcgccgcc gggggcacgt acgccgacct ggtcaaggag cactgacgtg gcagccatgt
     8521 cgtcgtcccc ctcgtccccc tcaccggtcc ccgccgcggg gcccggcggg accgacgcgg
     8581 tgacgcaggc cctgggcggc ctcggcacgc ccgtccagtg cgccggcctg cgcagcgtcg
     8641 ccctggaggg accgcacgtc ctctggctcg tcaccggggg cgcgctcgac ctcttcgcgg
     8701 tcgacgccgc cgccgagggc cactggcact tcctcggccg gctggaagcc ggcaccctgc
     8761 tgctgggccc ggtcgacggc ccccagcaca ccctgctcgg ccgcccctcg caggactgcg
     8821 ggctgcgccg gatcccgctg cgcgagctgc cccgccacga ctacggcgcc tacggcgagt
     8881 acggggagta cggggacacc ggctcctacc cgctgtactc cacccaggcc gagcagccct
     8941 ccgcgctgga gcacgccttc gccctgggca ccgcccgcag cctgggtgtg ctgttcgagg
     9001 cgccgctgga cggccggccc ggtgacgagg cggtcgccga cgacgacgtg ctgtggatgc
     9061 ccgtcccgcc cggcagcgtg cagtacggcg cctcgtacag cgcggaagcg gccgccgatc
     9121 tgctggtcga ccccgagctg tggcagcgga tggtcaacca gcagtaccgg ctgctgtccg
     9181 ccgtcgacgg ctggatcgag cacctggagc gtgcccacga ggaccgcgcg gcggccggca
     9241 tcaaggccgg cgaggcggtc cgcgagcgtg ccgaccaggc cctgctggcc tccatcggcc
     9301 gccaggaccg gtccgggcgg ggcggcgacg ccaccaaccg ggccggcgac gacaccacct
     9361 tcgaggtctg ccgggcggtc gccgaggcgg ccgggatcac gctcaccgca cctccgcggg
     9421 gcggcaccgt caacgagcgc gtcagccccg tggagcggat cgcggtcagc tcgcggatcc
     9481 gcacccgcgc ggtccgactc accggctcct ggtggcggac ggacgccggc ccgctggtgg
     9541 gccaccgcgc caagtccggt gcccccgtag cgctgctgtg gcgccgcggc cgctacgagg
     9601 ccgtcaaccc cgcgtccggg ctgcgaatcc gcgtcggcaa ggacaacgcc gacgagttcg
     9661 agccgcgcgc ggtgatgttc taccgcccgc tgccggagcg gccgatgaac atgtggcggc
     9721 tgatgcgctt cagtctgcgc ggcagccgga cggacgtccg caacctgacg ctcagcggcc
     9781 tggtggcggt cggcctcggc gcgctcgtcc cgatcgcgac cggcaaggtg ctcggtgtgt
     9841 acgtaccggc cgccgagcgg agcctgatcg tccaggtgtc cctggccgtc atcgtcacca
     9901 gcatcgtctc ggccgccttc atgctgctcc agaacctcac cgtgctgcgg atggagggcc
     9961 gcatcgagag cgcgctccag ccggccgtct gggaccggct gctgcggctg ccgacgaagt
    10021 tcttcaccga gcgctccacc ggcgagctgg ccagtgccgc gatgggcatc agcgcgatcc
    10081 ggcgggtgct gtccggcatc ggaccggtgg ccgtgcagtc caccacgatc ggcgcgatga
    10141 acctcgtcct gctgctcgtc tacagcgtgc cgctggcgct cgccgcgctc gccatgctgc
    10201 tggtcatcgg cgc
//
