LOCUS       NZ_CP017157            10417 bp    DNA     linear   CON 31-MAY-2024
DEFINITION  Streptomyces lydicus strain 103 chromosome, complete genome.
ACCESSION   NZ_CP017157
VERSION     NZ_CP017157.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces lydicus
  ORGANISM  Streptomyces lydicus
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 8201357)
  AUTHORS   Jia,N., Ding,M.-Z., Gao,F. and Yuan,Y.-J.
  TITLE     Complete genome sequencing of Streptomyces lydicus 103 and metabolic
            pathways analysis of antibiotic biosynthesis
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8201357)
  AUTHORS   Jia,N., Ding,M.-Z., Gao,F. and Yuan,Y.-J.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2016) Key Laboratory of Systems Bioengineering
            (Ministry of Education), Tianjin University, Tianjin 300072, PR
            China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SMRT Analysis v. 2.3.0
            Genome Coverage                   :: 100x
            Sequencing Technology             :: PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_001729485.1-RS_2024_05_31
            Annotation Date                   :: 05/31/2024 02:23:15
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,288
            CDSs (total)                      :: 7,195
            Genes (coding)                    :: 7,061
            CDSs (with protein)               :: 7,061
            Genes (RNA)                       :: 93
            rRNAs                             :: 7, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 47 of 134
            Pseudo Genes (incomplete)         :: 104 of 134
            Pseudo Genes (internal stop)      :: 11 of 134
            Pseudo Genes (multiple problems)  :: 26 of 134
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 01:52:31
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 2288691
            Orig. end                         :: 2299108
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP017157.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Source DNA is available from Prof. Ying-Jin Yuan.
            Annotation modified by submitter.
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10417
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="ectoine"
                     /region_number="5"
                     /rules="ectoine_synt"
                     /tool="antismash"
     cand_cluster    1..10417
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="ectoine_synt"
                     /kind="single"
                     /product="ectoine"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10417
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5417](-)"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5417)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
     gene            281..1351
                     /locus_tag="SL103_RS09815"
                     /old_locus_tag="SL103_09815"
     CDS             281..1351
                     /NRPS_PKS="Domain: Aminotran_5 (16-351). E-value: 9.9e-22.
                     Score: 69.3. Matches aSDomain:
                     nrpspksdomains_SL103_RS09815_Aminotran_5.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_5"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1139: aminotransferase class V"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003985687.1"
                     /locus_tag="SL103_RS09815"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SL103_09815"
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="WP_069568361.1"
                     /sec_met_domain="Aminotran_5 (E-value: 9.9e-14, bitscore:
                     49.7, seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MQRHEPLGGAEFAPETTYLNSASSGLLPRRSAAAVRAALDESASH
                     GTMGRDYFAAAHASRAAFARLVDVPAERVALGSSVAVRSALIAASLPAGAEVLAPEGDF
                     SSLVNPLGTHPGIKLRTVPLEALADEVRPGTALVAFSAVQSLDGRIADLAAVREAARTH
                     GARTFVDATQAAGWLPLRALDFDYLVAGAFKWLLCPRGTTFMAFGGERGDRTDGPGWPA
                     ALHAGWVAGEDLAASNYGLITPATTARRFDEPHAYLSYVGAEQSLSLIEELGVPAIHAH
                     DTALAAYYRERLTDRGLSPRPAPGSAIVSTPGLTDAEDRLAAAGVKISVRGGLLRAAFH
                     LYNSTDDVDRLLSLLD"
     aSDomain        329..1333
                     /aSDomain="Aminotran_5"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_SL103_RS09815_Aminotran_5.1"
                     /evalue="9.90E-22"
                     /label="SL103_RS09815_Aminotran_5.1"
                     /locus_tag="SL103_RS09815"
                     /protein_end="351"
                     /protein_start="16"
                     /score="69.3"
                     /tool="antismash"
                     /translation="TYLNSASSGLLPRRSAAAVRAALDESASHGTMGRDYFAAAHASRA
                     AFARLVDVPAERVALGSSVAVRSALIAASLPAGAEVLAPEGDFSSLVNPLGTHPGIKLR
                     TVPLEALADEVRPGTALVAFSAVQSLDGRIADLAAVREAARTHGARTFVDATQAAGWLP
                     LRALDFDYLVAGAFKWLLCPRGTTFMAFGGERGDRTDGPGWPAALHAGWVAGEDLAASN
                     YGLITPATTARRFDEPHAYLSYVGAEQSLSLIEELGVPAIHAHDTALAAYYRERLTDRG
                     LSPRPAPGSAIVSTPGLTDAEDRLAAAGVKISVRGGLLRAAFHLYNSTDDVDRL"
     gene            1455..2522
                     /locus_tag="SL103_RS09820"
                     /old_locus_tag="SL103_09820"
     CDS             1455..2522
                     /GO_function="GO:0010181 - FMN binding [Evidence IEA]"
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1217: NADH:flavin oxidoreductase/NADH oxidase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005319060.1"
                     /locus_tag="SL103_RS09820"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SL103_09820"
                     /product="alkene reductase"
                     /protein_id="WP_069568362.1"
                     /transl_table=11
                     /translation="MTTAFDPVDLAGIPLANRLVMAPMTRNRATADGVPTPSMVRYYTQ
                     RASAGLIITEATQTTPVGRGYPLTPGLHTPEQVAGWRAVTDSVHAAGGTIFAQLWHGGR
                     IGHPVLLPDGLVPVGPSPVAAAGQGFTHEGLKDFVTPRELTGQEIADTIAGFATAARNA
                     LDAGFDGVEIHGANGYLIHQFLAPNSNLRTDEWGGSDEARARFAIEVVRAVADAIGAHR
                     TGLRLSPGNPFNDISEPAPEATYTALVEAVAPLGLAYLHTCDVVDQDLLARLRKRFGGT
                     FVLNPATEGRPTGPEELPLVEEGRADLLAYGALFIANPDLPRRLAAGGPFNTPDRASFY
                     GGDDSGYLDYPALTD"
     gene            complement(2586..3908)
                     /locus_tag="SL103_RS09825"
                     /old_locus_tag="SL103_09825"
     CDS             complement(2586..3908)
                     /NRPS_PKS="Domain: Abhydrolase_1 (47-195). E-value:
                     1.1e-14. Score: 46.6. Matches aSDomain:
                     nrpspksdomains_SL103_RS09825_Abhydrolase_1.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF00561"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014145299.1"
                     /locus_tag="SL103_RS09825"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SL103_09825"
                     /product="alpha/beta fold hydrolase"
                     /protein_id="WP_069568363.1"
                     /sec_met_domain="PF00561 (E-value: 9.5e-14, bitscore: 50.3,
                     seeds: 48, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRADRMPGVVARDHVLQVPLDHDDPGGERLEVYGREVVAAGRERD
                     DLPWLVYLQGGPGCPSPRPVGRESWLTRALEDHRVLLLDQRGTGRSTPANRQTLPLRGG
                     PEQQAAYLAHFRADAIVRDAELFRRHLVPDGGPWSVLGQSFGGFCTVGYLSRAPEALSA
                     AYVTGGLPGLDASAEDVYRAAYPRVARKNAAHYARYPGDVAAVRRIVGHLREHVVRLPG
                     GGLLTAEAFLSLGLMLGSGDGSHTLHHLVSEAWVAGPAGPEPADAFLEAVQGHLSHAAT
                     PLFAVLHETIYGQRSVDAGPTGWAAERVRRQLAPSYAAFDVDAALAGDGPVYFTGEMMY
                     PWMFDTDPALRPLKETAQALAERTDWPDLYDAGRLAGNEVPAAAAVYFDDMYVDTGHAL
                     RTAERIRGLRVWVTNEWEHDGLRVSDGAVLDRLVRMVRGEV"
     aSDomain        complement(3324..3767)
                     /aSDomain="Abhydrolase_1"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_SL103_RS09825_Abhydrolase_1.1"
                     /evalue="1.10E-14"
                     /label="SL103_RS09825_Abhydrolase_1.1"
                     /locus_tag="SL103_RS09825"
                     /protein_end="195"
                     /protein_start="47"
                     /score="46.6"
                     /tool="antismash"
                     /translation="PWLVYLQGGPGCPSPRPVGRESWLTRALEDHRVLLLDQRGTGRST
                     PANRQTLPLRGGPEQQAAYLAHFRADAIVRDAELFRRHLVPDGGPWSVLGQSFGGFCTV
                     GYLSRAPEALSAAYVTGGLPGLDASAEDVYRAAYPRVARKNAAH"
     gene            complement(4005..4904)
                     /gene="thpD"
                     /locus_tag="SL103_RS09830"
                     /old_locus_tag="SL103_09830"
     CDS             complement(4005..4904)
                     /EC_number="1.14.11.55"
                     /codon_start=1
                     /gene="thpD"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PhyH"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006606339.1"
                     /locus_tag="SL103_RS09830"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SL103_09830"
                     /product="ectoine hydroxylase"
                     /protein_id="WP_069568365.1"
                     /sec_met_domain="PhyH (E-value: 1.9e-37, bitscore: 128.3,
                     seeds: 39, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTTAPERTADLYPTRGTNEVITPRKDPVVWSQPGAAGPFAPSELS
                     DFERDGFFAIGELLTAEEVAVYRAELDRLVLDPEMRADPRSIVEPKSQSVRSVFEVHKI
                     SEVFAKLVSDPRVVGRARQILGSDVYVHQSRINVKPGFGASGFYWHSDFETWHAEDGLP
                     NMRTVSVSIALTENYATNGGLMIMPGSHQHFVGCEGETPKDNYKRSLQMQDAGIPSDEV
                     LTKMADKHSIRLFTGKAGSATWFDCNAMHGSGDNITPYPRSNVFIVFNSVENAAVEPFA
                     APVRRPDYIGARDFTPVH"
     gene            complement(5001..5417)
                     /locus_tag="SL103_RS09835"
                     /old_locus_tag="SL103_09835"
     CDS             complement(5001..5417)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     ectoine: ectoine_synt"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006606340.1"
                     /locus_tag="SL103_RS09835"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SL103_09835"
                     /product="ectoine synthase"
                     /protein_id="WP_069568366.1"
                     /sec_met_domain="ectoine_synt (E-value: 9.3e-53, bitscore:
                     176.1, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIVRSFKDIEGTDRHVKAKSGTWESKRIVLAKERVGFSLHETVLY
                     AGTETSMWYANHIEAVVCVEGEAELTNDETGEKHTITPGVMYLLDGHEKHTMRIKEDFR
                     CLCVFNPPVTGREDHDENGVYPVLTEPDPEPEKV"
     gene            complement(5465..6733)
                     /gene="ectB"
                     /locus_tag="SL103_RS09840"
                     /old_locus_tag="SL103_09840"
     CDS             complement(5465..6733)
                     /EC_number="2.6.1.76"
                     /GO_function="GO:0045303 - diaminobutyrate-2-oxoglutarate
                     transaminase activity [Evidence IEA]"
                     /NRPS_PKS="Domain: Aminotran_3 (28-364). E-value: 5.9e-75.
                     Score: 244.3. Matches aSDomain:
                     nrpspksdomains_SL103_RS09840_Aminotran_3.1"
                     /codon_start=1
                     /gene="ectB"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_3"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1013: aminotransferase class-III"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007381147.1"
                     /locus_tag="SL103_RS09840"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SL103_09840"
                     /product="diaminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="WP_069568368.1"
                     /sec_met_domain="Aminotran_3 (E-value: 1.4e-75, bitscore:
                     253.2, seeds: 13, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTITQPDLSVFETVESEVRSYCRGWPTVFDRAQGSRLIDEDGHTY
                     LDFFSGAGSLNYGHNNPVLKRALIDYIERDGITHGLDMSTTAKRAFLESFQNIILRPRD
                     LPYKVMFPGPTGANSVEAALKLARKVKGRESIVSFTNAFHGMSLGALAVTGNSMKRAGA
                     GIPLVHGTPMPFDNYLDGAYPDFLWFERLLEDQGSGLNTPAAVIVETVQGEGGINVARA
                     EWLRALADLCKRRDMLLIVDDIQMGCGRTGAFFSFEEAGIVPDIVTVSKSISGYGLPLA
                     LTLFKPELDIWEPGEHNGTFRGNNPAFVTAAAALDTYWADGQMEKQTLARGEIVEAHLK
                     AIVEEHPGAFAEYRGRGLVWGLECIDKSLAGRIAARCFELGLLIETSGPESEVVKLLPA
                     LTTTPEELDEGLRTLGRAVRDCV"
     aSDomain        complement(5642..6649)
                     /aSDomain="Aminotran_3"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_SL103_RS09840_Aminotran_3.1"
                     /evalue="5.90E-75"
                     /label="SL103_RS09840_Aminotran_3.1"
                     /locus_tag="SL103_RS09840"
                     /protein_end="364"
                     /protein_start="28"
                     /score="244.3"
                     /tool="antismash"
                     /translation="FDRAQGSRLIDEDGHTYLDFFSGAGSLNYGHNNPVLKRALIDYIE
                     RDGITHGLDMSTTAKRAFLESFQNIILRPRDLPYKVMFPGPTGANSVEAALKLARKVKG
                     RESIVSFTNAFHGMSLGALAVTGNSMKRAGAGIPLVHGTPMPFDNYLDGAYPDFLWFER
                     LLEDQGSGLNTPAAVIVETVQGEGGINVARAEWLRALADLCKRRDMLLIVDDIQMGCGR
                     TGAFFSFEEAGIVPDIVTVSKSISGYGLPLALTLFKPELDIWEPGEHNGTFRGNNPAFV
                     TAAAALDTYWADGQMEKQTLARGEIVEAHLKAIVEEHPGAFAEYRGRGLVWGLEC"
     gene            complement(6859..7425)
                     /gene="ectA"
                     /locus_tag="SL103_RS09845"
                     /old_locus_tag="SL103_09845"
     CDS             complement(6859..7425)
                     /EC_number="2.3.1.178"
                     /GO_function="GO:0033816 - diaminobutyrate
                     acetyltransferase activity [Evidence IEA]"
                     /GO_process="GO:0019491 - ectoine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="ectA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016577188.1"
                     /locus_tag="SL103_RS09845"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SL103_09845"
                     /product="diaminobutyrate acetyltransferase"
                     /protein_id="WP_208869843.1"
                     /transl_table=11
                     /translation="MTAAQADHAGARSDIREIPEGFKLDTPRVEDGAAIWRIARDSKAL
                     DLNSSYSYLLWCRDFAATSVVARDAEGEPAAFLTGYLRPERPETLVVWQIAVDDAHRGK
                     GLAAALLDGLTLRARDELGIRYVETTITPDNAASNRLFASFAERHSVPVTREVLFDAGL
                     FPEQGHEPEVLHLIGPFEPPAPAPG"
     misc_feature    7580..7595
                     /note="TFBS match to CRP, Cyclic AMP receptor protein,
                     confidence: medium, score: 16.52"
                     /tool="antismash"
     misc_feature    7590..7605
                     /note="TFBS match to AfsQ1, Two-component system AfsQ1-Q2,
                     activator of antibiotic production, confidence: medium,
                     score: 17.62"
                     /tool="antismash"
     misc_feature    complement(7683..7703)
                     /note="TFBS match to MntR, Manganese transport regulator,
                     confidence: weak, score: 19.68"
                     /tool="antismash"
     gene            7911..9011
                     /locus_tag="SL103_RS09850"
                     /old_locus_tag="SL103_09850"
     CDS             7911..9011
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Amidohydro_1"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006123619.1"
                     /locus_tag="SL103_RS09850"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SL103_09850"
                     /product="amidohydrolase family protein"
                     /protein_id="WP_069568370.1"
                     /sec_met_domain="Amidohydro_1 (E-value: 4.5e-16, bitscore:
                     57.7, seeds: 57, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTSKSEGAVLHLKGRVLVGPDDVRDELWVVGGRVTFDRPAMARDA
                     TTVEGWALPGLVDAHCHVGLDAHGPVDAPTSEKQALTDRDAGTLLIRDAGSPSDTRWVD
                     DREDLPRIIRAGRHIARTKRYIRNYAHEIEPEDLVAYVAQEARRGDGWVKLVGDWIDRS
                     TGDLSACWPRGAVEAAIAEAHRLGARVTAHCFAEESLAPLVEAGIDCIEHATGLTEDTI
                     PLFAERGVAIVPTLINIATFPQLAAGGQAKYPRWADHMRRLHERRYETVRAAYDAGVPV
                     FAGTDAGGSLAHGLVAEEVLELTRAGLPAVAALSATTWGARDWLGRPGLTEGASADLVV
                     YESDPREDVRVLAAPRRVVLRGQVVG"
     gene            9341..10330
                     /locus_tag="SL103_RS09855"
                     /old_locus_tag="SL103_09855"
     CDS             9341..10330
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018536882.1"
                     /locus_tag="SL103_RS09855"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SL103_09855"
                     /product="SCO1860 family LAETG-anchored protein"
                     /protein_id="WP_069573583.1"
                     /transl_table=11
                     /translation="MPRRLAATLVATALTAGPAVLLGAAPAHATGDHGPARGTSGAVVL
                     RAGLNVGLLNKTLDVPLNAVLNEVHAPASANKTALTVNLDGIGHGKPFSVLRADVATAR
                     ATADHRKAEGYANLVHAEVHLPGLPLLSLIEVQQVTATAVCEAGKAPRATSNVLGSVRV
                     LGKKVTLTAGGTTEVKVPGVGEVRLDLSKRSTTARKAAATALDLNVSVNPLKLNVADVR
                     GRVTLAQATCTTPGGKTPRPGGSGGTGGGQPQPSGGTRPAGDSKPAGGKDTQVNQPGSR
                     PSTQNLAETGGSSATPYLAGGAALLVVAGAGSMVYARRRTAARQTGRG"
ORIGIN
        1 cgtacgccca ggaggtacgc gccgacgagc gcgaggccgc cgcgctcggc gccaccggcg
       61 tccccttctt cgtcatcgac cgccgctacg gcatctccgg cggccagccc gccgacgtct
      121 tccgccaggc cctggagcgg gcccacgccg acggcgcgat ccagaccctc gccgcccccg
      181 acggggaggt ctgcggcgac gacggctgcg ccgtacccgg cgtctgacac cgtccgaaag
      241 acaatggacg cgaccggctc ccgccgcgca cagtggggtc atgcagcgac atgagccact
      301 cggcggggcc gaattcgcgc ccgagacgac ctacctcaac tccgcctcca gcggactgct
      361 cccgcgccgc agcgccgcag ccgtgcgcgc ggccctcgac gaatccgcct cgcacggcac
      421 gatgggccgc gactacttcg ccgccgcgca cgcctcccgg gccgccttcg cccggctggt
      481 ggacgtcccc gccgagcggg tcgccctcgg cagctccgtc gccgtccggt ccgcgctgat
      541 cgccgcgtcc ctcccggcgg gcgccgaagt cctcgcaccc gagggcgact tcagctccct
      601 ggtcaacccg ctcggcaccc acccgggcat caagctgcgc accgtgcccc tggaagcgct
      661 cgccgacgag gtgcgccccg gcaccgcgct ggtcgccttc agcgccgtcc agtcgctcga
      721 cggccggatc gccgacctcg ccgccgtacg ggaagccgcc cggacccacg gggcgcgcac
      781 cttcgtcgac gccacccagg ccgccggctg gctgccgctg cgcgccctcg acttcgacta
      841 cctcgtcgcc ggcgccttca aatggctgct gtgcccgcgc ggcaccacct tcatggcctt
      901 cggcggcgag cgcggcgacc gcaccgacgg ccccggctgg ccggccgcgc tgcacgccgg
      961 ctgggtcgcc ggcgaggacc tcgccgcgtc caactacgga ctgatcaccc cggccaccac
     1021 cgcccgccgc ttcgacgaac cgcacgccta cctctcgtac gtcggcgccg aacagtccct
     1081 ctcgctcatc gaggaactgg gcgtacccgc gatccacgcc cacgacaccg cgctcgccgc
     1141 gtactaccgc gagcggctca ccgaccgcgg cctcagcccg cgccccgcac ccggctcggc
     1201 catcgtctcc acccccggcc tcacggacgc cgaggaccgg ctcgccgcgg ccggcgtgaa
     1261 gatctccgta cgcggcgggc tgctgcgcgc ggccttccac ctctacaact ccaccgacga
     1321 cgtcgaccgg ctgctgtccc tgctggactg aagcggacca cggcaccggg aatgcgggac
     1381 cccgccggcg atgttggcac cgctgacgtc acgtctgatc acgtgcaccg gacagcagtt
     1441 gagaggcccc ttcagtgacc accgctttcg accccgtcga cctcgccggc atcccgctcg
     1501 ccaaccgcct cgtgatggcg ccgatgaccc gcaaccgcgc caccgccgac ggcgtcccca
     1561 ccccgtcgat ggtccgctac tacacccagc gggcctccgc cggactcatc atcaccgaag
     1621 ccacccagac cacccccgtc ggccgcggct accccctcac cccgggcctg cacacccccg
     1681 agcaggtcgc cggatggcgt gcggtcaccg actccgtgca cgccgcgggc ggcacgatct
     1741 tcgcgcagct gtggcacggc ggccggatcg gccaccccgt gctgctgccc gacggcctgg
     1801 tgccggtcgg cccctcaccg gtcgccgcgg ccggccaggg cttcacccac gagggcctca
     1861 aggacttcgt cacgccccgc gagctgaccg gccaggagat agccgacacg atcgccggct
     1921 tcgccaccgc cgcccgcaac gccctcgacg cgggcttcga cggcgtcgag atccacggcg
     1981 ccaacggcta cctcatccac cagttcctcg cgcccaacag caacctgcgc accgacgagt
     2041 ggggcggctc cgacgaggcc cgcgcccgct tcgccatcga ggtcgtacgg gccgtcgccg
     2101 acgcgatcgg cgcccaccgc accggcctgc gcctctctcc cgggaacccc ttcaacgaca
     2161 tctccgagcc cgcccccgaa gccacctaca ccgcgctcgt cgaagcggtc gccccgctgg
     2221 gcctggccta cctgcacacc tgcgacgtcg tcgaccagga cctgctggcc cggctgcgca
     2281 agcgcttcgg cgggacgttc gtcctcaacc ccgccaccga aggccgcccc accggccccg
     2341 aggagctccc gctcgtcgag gagggccggg ccgacctgct ggcctacggc gcgctcttca
     2401 tcgccaaccc cgacctgccc cgccgtctcg ccgccggcgg ccccttcaac acccccgacc
     2461 gcgccagctt ctacggcggc gacgacagcg gctacctcga ctacccggcc ctgaccgact
     2521 gagcacccac cgacgcaccg gcggggcgca ccgtgcgccc cgccggcccg ctcacacctc
     2581 tcggctcaca cctctccgcg caccatccgc accagccggt ccaggaccgc cccgtcactg
     2641 acccgcagtc cgtcgtgctc ccactcgttc gtcacccaca cccgcagccc ccggatccgc
     2701 tccgcggtgc gcagcgcatg accggtgtcc acgtacatgt cgtcgaagta gaccgccgcc
     2761 gcggccggca cctcgttgcc ggccagccgc ccggcgtcgt acagatccgg ccagtccgtc
     2821 cgctccgcca gcgcctgcgc ggtctccttc aacggccgca gcgccggatc ggtgtcgaac
     2881 atccacgggt acatcatctc gccggtgaag tacaccggcc cgtccccggc cagcgccgcg
     2941 tccacgtcga acgccgcgta cgacggggcc agttggcgcc gcacccgctc cgccgcccag
     3001 cccgtcggcc ccgcgtccac cgaccgctgc ccgtagatcg tctcgtgcag caccgcgaac
     3061 aacggcgtcg cggcatgcga gagatgaccc tgcacggcct ccaggaacgc gtccgccggc
     3121 tccgggccgg ccgggcccgc cacccacgcc tcactcacca ggtggtgcag ggtgtgcgac
     3181 ccgtcgccgc tgcccagcat cagccccagc gacaggaacg cctccgcggt cagcagcccc
     3241 ccgccgggca gccgcaccac gtgctcgcgc agatgcccca cgatccgccg caccgcggcc
     3301 acgtcccccg gataccgcgc gtagtgcgcc gcgttcttcc gcgccacccg cggatacgcc
     3361 gcccggtaga cgtcctcggc actcgcgtcc agaccgggca gcccgcccgt gacgtacgcc
     3421 gccgacagcg cctccggcgc ccgggagagg taaccgaccg tgcagaaccc gccgaagctc
     3481 tggcccagca cgctccacgg cccgccgtcc gggaccaggt ggcggcggaa cagctcggcg
     3541 tcccgcacga tcgcgtccgc ccggaagtgc gccaggtacg ccgcctgctg ctccgggccg
     3601 ccgcgcagcg gcagcgtctg ccggttcgcc ggcgtcgacc gccccgtacc gcgctggtcc
     3661 agcagcagca cccggtggtc ctccagcgcc cgcgtcagcc aggactcccg ccccaccgga
     3721 cgcggcgacg gacagcccgg accgccctgc aggtacacca gccacggcag gtcgtcccgc
     3781 tcccgcccgg ccgccaccac ctcgcggccg tacacctcca gccgctcgcc gcccggatcg
     3841 tcatgatcca gtggcacctg aagcacatgg tcgcgcgcca cgacccccgg catccggtcc
     3901 gccctcatca gcccctcctc gcacacgacg cggcgcggcc gccgcacaga ccctgctgtg
     3961 cgacgaccgc gccaccggta ccgctctcgt ctacgtcacc gtactcagtg caccggggtg
     4021 aagtcccgcg ctccgatgta atccgggcgc cgcaccggcg ccgcgaacgg ctccaccgcc
     4081 gcgttctcca cgctgttgaa gacgatgaag acattgctgc gggggtacgg cgtgatgttg
     4141 tcacccgagc cgtgcatggc gttgcagtcg aaccacgtcg ccgaaccggc cttgcccgtg
     4201 aacagccgga tgctgtgctt gtccgccatc ttcgtcagca cctcgtccga cgggatgccc
     4261 gcgtcctgca tctggagcga ccgcttgtag ttgtccttcg gcgtctcgcc ctcgcagccc
     4321 acgaagtgct ggtgcgaacc aggcatgatc atcaggccgc cgttggtggc gtagttctcg
     4381 gtcagcgcga tcgacacgga cacggtccgc atgttcggca gcccgtcctc cgcgtgccac
     4441 gtctcgaagt ccgagtgcca gtagaagccg gaagcaccga agccgggctt cacgttgatc
     4501 cgcgactggt ggacgtagac gtccgagccg aggatctgcc gggcccggcc gaccacacgc
     4561 ggatcggaga ccagcttggc gaacacctca ctgatcttgt gcacctcgaa gacggaccgg
     4621 acgctctgcg acttcggctc gacgatggag cgcgggtcgg cgcgcatctc cgggtcgagc
     4681 accagccggt ccagctcggc gcggtacacc gcgacttctt ccgccgtcag cagttccccg
     4741 atggcgaaaa agccgtcgcg ttcgaagtcg ctcagctcgg acggcgcgaa cgggccggcc
     4801 gctccgggct gcgaccacac caccgggtcc ttccgcgggg tgatgacctc attggtccca
     4861 cgggtcgggt acaggtcggc ggtgcgctcg ggtgcggtgg tcatggtgtt gccttcctct
     4921 cctctcgtac ggaatccggc tcacgtgcgt gacgcggggc ttccgcggcc ggcccgcgga
     4981 ggggccggcc gtggatcgcc tcagaccttc tccggctccg ggtccggctc ggtgagaacc
     5041 gggtagaccc cgttctcgtc gtggtcctcg cggccggtga ccggcgggtt gaagacgcac
     5101 aggcagcgga agtcttcctt gatccgcatg gtgtgcttct cgtgcccgtc gagcaggtac
     5161 atcacgccgg gcgtgatggt gtgcttctcg ccggtctcgt cgttggtcag ctcggcctcg
     5221 ccctcgacgc agacgacggc ctcgatgtgg ttcgcgtacc acatcgacgt ctccgtaccg
     5281 gcgtacagga cggtctcgtg cagcgagaac ccgacgcgct ccttggcgag cacgatgcgc
     5341 ttgctctccc acgtaccgga cttggccttg acgtggcggt cggtgccctc gatgtccttg
     5401 aaggatcgga cgatcacggt gggtcggtgc ctttctgtcg aacgtgcagg aactctgacg
     5461 ggtctcagac gcagtcgcgg accgcgcggc cgagggtccg aaggccctcg tccagctcct
     5521 cgggggtggt ggtcagggcg ggcagcagtt tgacgacctc gctctccgga ccggaggtct
     5581 cgatcagcag gcccagctcg aagcaccggg ccgcgatccg gcccgcgagg ctcttgtcga
     5641 tgcactccag accccacacc agaccgcggc cgcggtactc ggcgaaggcg cccgggtgct
     5701 cctcgacgat ggccttcagg tgtgcttcga cgatctcacc gcgggcgagg gtctgcttct
     5761 ccatctggcc gtcggcccag taggtgtcca gcgcggcggc ggccgtcacg aaggccgggt
     5821 tgttgccgcg gaacgtgccg ttgtgctcgc cgggctccca gatgtccagc tcaggcttga
     5881 acagggtgag cgccagcggc agcccgtagc cgctgatcga cttcgagacg gtgacgatgt
     5941 ccggcacgat gcccgcctcc tcgaaggaga agaaggcacc cgtacggccg cagcccatct
     6001 ggatgtcgtc gacgatcagc agcatgtccc ggcgcttgca caggtcggcc agcgcccgca
     6061 gccactcggc gcgggccacg ttgatgccgc cctcgccctg gaccgtctcc acgatcaccg
     6121 cggcgggcgt gttcaggccg gaaccctggt cctccagcag ccgctcgaac cacaggaagt
     6181 ccgggtacgc cccgtccagg tagttgtcga acggcatcgg ggtgccgtgc accagcggga
     6241 taccggcgcc ggcccgcttc atcgagttgc cggtgaccgc gagcgcgccc agcgacatgc
     6301 cgtggaaggc gttggtgaag gacacgatcg actcgcggcc cttgaccttg cgggcgagct
     6361 tcagcgccgc ctcgaccgag ttggcgcccg tcggcccggg gaacatgacc ttgtacggca
     6421 ggtcgcgcgg ccgcaggatg atgttctgga acgactccag gaacgcccgc ttggccgtgg
     6481 tggacatgtc caggccgtgg gtgatgccgt cccgctcgat gtagtcgatc agggcgcgtt
     6541 tgaggaccgg gttgttgtgt ccgtagttga gcgacccggc tccggagaag aagtccaggt
     6601 aggtgtgccc gtcctcgtcg atcagacggc tgccctgggc gcggtcgaag acggtgggcc
     6661 agccacggca gtagctgcgc acctccgact ccacggtctc gaagacgctc agatcgggct
     6721 gggtgatggt cacagcatgc tcctgggaga gagtgcgaag gatgaggaag tctgggggcg
     6781 ggggtgccgg cggcccttgt gggcggccgg atccgcgggg ccggcgcggc gccgtcgccg
     6841 cgcggcccgg gggcgctact acccgggggc aggggcggga ggctcgaacg ggcctatgag
     6901 gtgcagcacc tccggctcgt ggccctgctc ggggaacagc cccgcgtcga agagcacctc
     6961 gcgcgtgacc ggcacggagt gccgctcggc gaaggacgcg aacaacctgt tggacgcggc
     7021 gttgtcgggt gtgatggtgg tctcgacgta acgaatgccc agctcgtccc tggcgcggag
     7081 cgtcagcccg tccaggagtg cggccgccag ccccttgcca cggtgcgcgt cgtcgacggc
     7141 gatctgccag acgaccaggg tctccgggcg ctcggggcgg aggtagccgg tgaggaaggc
     7201 ggccggctcg ccctccgcgt cgcgggcgac gacggaggtg gcggcgaagt cacgacacca
     7261 cagcaggtag ctgtaggacg agttgaggtc cagcgccttc gagtcacggg cgatgcgcca
     7321 gatcgctgcc ccgtcctcca cacgtggagt gtcgagcttg aagccctccg gaatttctct
     7381 gatatcgctg cgggcacctg catggtctgc ttgtgcggcg gtcatgcaat tgaatttacc
     7441 caggcgaaaa ggaaattgca tcgcgggcag gggttacgta aacgagctgg atatgttatc
     7501 gcgcgggcgc gcctgtgcgt gcgatgtggc cgaaacttgt cacgatttgc ccaggaggtt
     7561 cggggcgaaa cggacacgct gtgtagtcgg tcacaggtgc gtaacgctcg cgagatccgt
     7621 ccgaattacg ggattcggca ctagcaaaat cttggcgttt gagctgcgga aaagcgggca
     7681 gaagaatacg ggaagctatg cttccaagaa attgggaatt cgccggtgga agaccccgcg
     7741 ttggccctat gtaaacctgt acggcaaacc catgacaagg cccgcaacac catgcgccgc
     7801 gatttgggaa acctccggga atgcccggct cccggtaccc gcggcgccgc gcggcaaaga
     7861 cccccggcac cccgccccac cggcccggcc gcgccgccta aagtgcctgt atgacgtcca
     7921 agagtgaggg tgcggtgctg cacctcaagg ggagggtcct cgtcgggccg gacgacgtac
     7981 gcgacgagct gtgggtggtc ggcggtcgtg tcaccttcga ccggccggcc atggcacgcg
     8041 atgccaccac ggtggagggc tgggcactgc ccggcctcgt cgacgcccac tgccacgtgg
     8101 gtctggacgc acacggcccg gtggacgccc ccaccagcga aaaacaggcg ctcacggacc
     8161 gtgacgccgg caccctgctg atccgcgacg cggggtcgcc ctccgacacc cgctgggtcg
     8221 acgaccgcga ggacctcccg cggatcatcc gggccggccg ccacatcgcc cgtaccaagc
     8281 gctacatccg caactacgcg cacgagatcg aacccgagga cctggtcgcc tacgtcgccc
     8341 aggaggctcg ccgcggcgac ggctgggtca aactcgtcgg cgactggatc gaccgctcca
     8401 ccggcgacct gtccgcctgc tggccccggg gcgcggtcga ggcggccatc gccgaggcgc
     8461 accggctcgg cgcccgggtg accgcgcact gcttcgccga ggagtccctc gcgcccctcg
     8521 tcgaggccgg catcgactgc atcgagcacg ccaccggcct caccgaggac accatcccgc
     8581 tgttcgccga acgcggcgtc gcgatcgtcc ccactctgat caacatcgcc acgttcccgc
     8641 agctcgcggc cggtggccag gcgaaatacc cgcgctgggc ggaccacatg cgccgcctcc
     8701 acgaacgccg ctacgagacc gtgcgggccg cctacgacgc cggtgtgccg gtcttcgccg
     8761 gcaccgacgc cggcggctcg ctggcccacg gcctggtcgc cgaggaggtc ctcgaactga
     8821 cccgggccgg cctgcccgcc gtcgccgccc tctccgccac cacctggggc gccagggact
     8881 ggctcggccg cccgggcctc accgagggcg cctccgccga cctcgtggtc tacgagagcg
     8941 atccgcgcga ggacgtacgc gtcctggcgg ccccgcgcag ggtcgtcctg cgcgggcagg
     9001 tggtcggcta gcgggccggg cctccgcggg gctccggggc ggcgcacggc gccgccccga
     9061 tccggggacc gggggaacga ggtcggtcaa cccgggtcac ccgtgcggtg gggcggctgt
     9121 gacgcgagag ccgcaccgtg ccgcaggaat cgaatgcaaa cgcgaaaacc accctttggg
     9181 atgaactgac cacgcgtggc tccccgttca ccctccgtgc ggacacgatg tgcgggtcga
     9241 ggtcgtcggc ccgcgtgatg tcccccattg gcgcggccgg cggctccatc tctcttgtgg
     9301 gggttccaca cacgtgtcca ccagtcccac ccactccggc atgcccaggc gcctcgccgc
     9361 gaccctcgtc gccacggccc tgacggccgg tcccgccgtc ctgctcggcg ccgcgcccgc
     9421 ccacgccacc ggcgaccacg gcccggcccg cggcacgtcc ggcgcggtcg tgctgcgcgc
     9481 cggcctgaac gtcggcctgc tcaacaagac gctcgacgtc ccgttgaacg ccgtcctcaa
     9541 cgaggtgcac gccccggcct ccgcgaacaa gaccgcgctc accgtcaacc tcgacggcat
     9601 cgggcacggc aagccgttca gcgtgctgcg cgccgacgtc gccaccgcac gggccaccgc
     9661 ggaccaccgc aaggccgagg gctacgccaa cctcgtgcac gccgaggtgc atctgccggg
     9721 cctgccgctg ctgtccctga tcgaggtcca gcaggtcacc gccaccgcgg tgtgcgaggc
     9781 cggcaaggcg ccccgcgcca cgtcgaacgt gctgggcagc gtccgcgtcc tgggcaagaa
     9841 ggtcacgctc acggcgggcg ggacgacgga ggtgaaggtg cccggcgtgg gcgaggtgcg
     9901 gctcgacctg tcgaagcgga gcaccacggc caggaaggcc gccgccaccg cgctcgacct
     9961 gaacgtctcc gtcaaccccc tgaagctcaa cgtcgccgac gtccgcggac gggtcacgct
    10021 cgcccaggcc acctgcacca ccccgggcgg caagacaccc cgtccgggcg gctccggcgg
    10081 caccggcggc ggtcagccgc aacccagcgg cggcacccgg ccggccgggg acagcaagcc
    10141 ggccggcggc aaggacaccc aggtcaacca gccgggctcc cggccgagca cccagaacct
    10201 cgccgaaacc ggcggcagct cggccacgcc gtacctcgcc ggcggcgcgg ccctgctcgt
    10261 cgtcgcgggc gccggctcga tggtctacgc ccgccgccgt accgccgccc ggcagaccgg
    10321 gcgcggctga cagccccgta ccccgggccg gcgctgtggg ggcgccgccc cggggtacgg
    10381 ccccgtatga cctacggggc ggacctaccg gggcccg
//
