LOCUS       NZ_CP012749            22816 bp    DNA     circular CON 05-MAR-2024
DEFINITION  Rhodococcus sp. 008 chromosome, complete genome.
ACCESSION   NZ_CP012749
VERSION     NZ_CP012749.1
KEYWORDS    RefSeq.
SOURCE      Rhodococcus sp. 008
  ORGANISM  Rhodococcus sp. 008
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Nocardiaceae; Rhodococcus.
REFERENCE   1  (bases 1 to 6570200)
  AUTHORS   Loewen,P.C. and Brassinga,K.
  TITLE     Genome sequence of a ureolytic Rhodococcus species isolated from
            skate fish
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6570200)
  AUTHORS   Loewen,P.C. and Brassinga,A.K.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-SEP-2015) Microbiology, University Manitoba, 418
            Buller Bdg, Winnipeg, Manitoba R3T 2N2, Canada
REFERENCE   3  (bases 1 to 6570200)
  AUTHORS   Loewen,P.C. and Brassinga,K.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-SEP-2015) Microbiology, University of Manitoba, 408
            Buller Building, Winnipeg, Manitoba R3T 2N2, Canada
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: smrtanalysis v. 2.3.0.140936
            Genome Coverage                   :: 176x
            Sequencing Technology             :: PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 03/04/2024 12:54:36
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,967
            CDSs (total)                      :: 6,896
            Genes (coding)                    :: 6,809
            CDSs (with protein)               :: 6,809
            Genes (RNA)                       :: 71
            rRNAs                             :: 5, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5, 5, 5 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 58 of 87
            Pseudo Genes (internal stop)      :: 13 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 16:00:06
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 4135709
            Orig. end                         :: 4158525
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP012749.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Source bacteria are available from Dr. A. K. Brassinga, Dept of
            Microbiology, University of Manitoba, Winnipeg, MB R3T 2N2, Canada.
            This genome has a base modification file available.
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..22816
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="redox-cofactor"
                     /region_number="9"
                     /rules="(cds(PF04055 and (TIGR04085 or SPASM)) and
                     (TIGR02111 or TIGR03966) and minimum(1,
                     [Bottromycin_Methyltransferase_RRE, Cyanobactin_D_RRE,
                     Cytolysin_C_RRE, Goadsporin_CD_RRE,
                     Heterocycloanthracin_C_RRE, Lanthipeptide_LanB_RRE,
                     Lasso_Fused_RRE, Mycofactocin_RRE, NHLP_CD_RRE,
                     Other_Sactipeptide_rSAM_RRE, Pantocin_Microcin_RRE,
                     Plantazolicin_C_RRE, PlpY_RRE, PqqD_RRE,
                     Proteusin_Epimerase_RRE, Proteusin_Methyltransferase_RRE,
                     Quinohemoprotein_rSAM_RRE, Ranthipeptide_rSAM_RRE,
                     Stand_Alone_Lasso_RRE, Streptide_RRE, Subtilosin_rSAM_RRE,
                     Thiaglutamate_B_RRE, Thiaglutamate_G_RRE,
                     Thiaglutamate_I_RRE, Thiopeptide_F_RRE, Thuricin_rSAM_RRE,
                     Thurincin_rSAM_RRE, Trifolitoxin_RRE]))"
                     /tool="antismash"
     cand_cluster    1..22816
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(cds(PF04055 and (TIGR04085 or SPASM))
                     and (TIGR02111 or TIGR03966) and minimum(1,
                     [Bottromycin_Methyltransferase_RRE, Cyanobactin_D_RRE,
                     Cytolysin_C_RRE, Goadsporin_CD_RRE,
                     Heterocycloanthracin_C_RRE, Lanthipeptide_LanB_RRE,
                     Lasso_Fused_RRE, Mycofactocin_RRE, NHLP_CD_RRE,
                     Other_Sactipeptide_rSAM_RRE, Pantocin_Microcin_RRE,
                     Plantazolicin_C_RRE, PlpY_RRE, PqqD_RRE,
                     Proteusin_Epimerase_RRE, Proteusin_Methyltransferase_RRE,
                     Quinohemoprotein_rSAM_RRE, Ranthipeptide_rSAM_RRE,
                     Stand_Alone_Lasso_RRE, Streptide_RRE, Subtilosin_rSAM_RRE,
                     Thiaglutamate_B_RRE, Thiaglutamate_G_RRE,
                     Thiaglutamate_I_RRE, Thiopeptide_F_RRE, Thuricin_rSAM_RRE,
                     Thurincin_rSAM_RRE, Trifolitoxin_RRE]))"
                     /kind="single"
                     /product="redox-cofactor"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..22816
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[10000:12816](+)"
                     /cutoff="20000"
                     /detection_rule="(cds(PF04055 and (TIGR04085 or SPASM)) and
                     (TIGR02111 or TIGR03966) and minimum(1,
                     [Bottromycin_Methyltransferase_RRE, Cyanobactin_D_RRE,
                     Cytolysin_C_RRE, Goadsporin_CD_RRE,
                     Heterocycloanthracin_C_RRE, Lanthipeptide_LanB_RRE,
                     Lasso_Fused_RRE, Mycofactocin_RRE, NHLP_CD_RRE,
                     Other_Sactipeptide_rSAM_RRE, Pantocin_Microcin_RRE,
                     Plantazolicin_C_RRE, PlpY_RRE, PqqD_RRE,
                     Proteusin_Epimerase_RRE, Proteusin_Methyltransferase_RRE,
                     Quinohemoprotein_rSAM_RRE, Ranthipeptide_rSAM_RRE,
                     Stand_Alone_Lasso_RRE, Streptide_RRE, Subtilosin_rSAM_RRE,
                     Thiaglutamate_B_RRE, Thiaglutamate_G_RRE,
                     Thiaglutamate_I_RRE, Thiopeptide_F_RRE, Thuricin_rSAM_RRE,
                     Thurincin_rSAM_RRE, Trifolitoxin_RRE]))"
                     /neighbourhood="10000"
                     /product="redox-cofactor"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      10001..12816
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(cds(PF04055 and (TIGR04085 or SPASM)) and
                     (TIGR02111 or TIGR03966) and minimum(1,
                     [Bottromycin_Methyltransferase_RRE, Cyanobactin_D_RRE,
                     Cytolysin_C_RRE, Goadsporin_CD_RRE,
                     Heterocycloanthracin_C_RRE, Lanthipeptide_LanB_RRE,
                     Lasso_Fused_RRE, Mycofactocin_RRE, NHLP_CD_RRE,
                     Other_Sactipeptide_rSAM_RRE, Pantocin_Microcin_RRE,
                     Plantazolicin_C_RRE, PlpY_RRE, PqqD_RRE,
                     Proteusin_Epimerase_RRE, Proteusin_Methyltransferase_RRE,
                     Quinohemoprotein_rSAM_RRE, Ranthipeptide_rSAM_RRE,
                     Stand_Alone_Lasso_RRE, Streptide_RRE, Subtilosin_rSAM_RRE,
                     Thiaglutamate_B_RRE, Thiaglutamate_G_RRE,
                     Thiaglutamate_I_RRE, Thiopeptide_F_RRE, Thuricin_rSAM_RRE,
                     Thurincin_rSAM_RRE, Trifolitoxin_RRE]))"
                     /neighbourhood="10000"
                     /product="redox-cofactor"
                     /protocluster_number="1"
     misc_feature    996..1014
                     /note="TFBS match to FuR_variant_2, Ferric Uptake
                     Regulator, confidence: weak, score: 22.94"
                     /tool="antismash"
     misc_feature    complement(1171..1182)
                     /note="TFBS match to CcpA, Catabolite control protein A,
                     confidence: medium, score: 17.0"
                     /tool="antismash"
     gene            1212..1895
                     /locus_tag="AOT96_RS19390"
                     /old_locus_tag="AOT96_19405"
     CDS             1212..1895
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1016: LuxR family
                     DNA-binding response regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019744264.1"
                     /locus_tag="AOT96_RS19390"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AOT96_19405"
                     /product="response regulator transcription factor"
                     /protein_id="WP_042453153.1"
                     /transl_table=11
                     /translation="MTTARPVITRGASPVLGLVRIVLVDDHAILRQGLRSVLERESDLE
                     IVGEASSLAEALAVVDKVGPDIVLMDLKLSASSDYEGLTLCGQLSSAHPGLGLLVLTTF
                     LDDQLVVRAVHAGARGYVVKDVDTTELVRAIRAVSRGESAFDSRSASAVVRSLNGQSSS
                     KERLTDRELEVLRLLANGLSNIKIGERLFISPTTVKFHVSNIMRKLEVSRRAEAVYAAS
                     KQGLI"
     gene            complement(1892..2431)
                     /locus_tag="AOT96_RS19395"
                     /old_locus_tag="AOT96_19410"
     CDS             complement(1892..2431)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003941864.1"
                     /locus_tag="AOT96_RS19395"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AOT96_19410"
                     /product="hypothetical protein"
                     /protein_id="WP_007728324.1"
                     /transl_table=11
                     /translation="MKLSIRGANTSPTPPFARTASPETCERSISLNRTAAEAFAGDHHA
                     LSKLLARLHPRVVLYYRARLLGTPVATFADDLAHQCTLDVVTRMSDTISADTRIETFAY
                     ELASRRIASIPQSRNIDGQFKRPRRLTDDQREVLVLRIAVGLTAEMTASTLRTTRSSVL
                     LLQHQALRAWTVSEQS"
     gene            complement(2515..3009)
                     /locus_tag="AOT96_RS19400"
                     /old_locus_tag="AOT96_19415"
     CDS             complement(2515..3009)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007728322.1"
                     /locus_tag="AOT96_RS19400"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AOT96_19415"
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_007728322.1"
                     /transl_table=11
                     /translation="MTDTSHTVAPDESRHYVERLSLVLSDMGFPPMPARVWAALMTSET
                     PSSTPGDLGSRLGVSPAAISGAVRYLQQVGLIERVAVPGSRRQHYCVSVDMWADAFVTK
                     QSSLYQVSAVADEGIRVLGEGTTAGDRMADLRDFFDFMAAEMPRMLADWRSRRKDRTPG
                     R"
     gene            3078..4013
                     /locus_tag="AOT96_RS19405"
                     /old_locus_tag="AOT96_19420"
     CDS             3078..4013
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019744261.1"
                     /locus_tag="AOT96_RS19405"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AOT96_19420"
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="WP_003942009.1"
                     /transl_table=11
                     /translation="MGAAISVKSLVKTFGTTRALDGLDLDVSTGEVHGFLGPNGSGKST
                     TIRVLLGLLRSDSGDATVLGGDPWRDAVTLHRRLAYVPGDVNLWPNLTGGEAIDLLAGL
                     RGGLDRKRRSELLDRFELDPTKKARTYSKGNRQKVALVAAFSSDVDLYVLDEPTSGLDP
                     LMEAVFQDCVEEVAHAGKTVLLSSHILAEVEALCDRVSIIRDGRTVEEGTLSELRHLTR
                     TSVAAETQRPIGGLDGLGGVHDAVIDGNHVTCEVDSAQLGPLMTYLVGFGIVALTSTPP
                     TLEELFLRHYGDERARETSVHLRAETGVRP"
     gene            4010..5680
                     /locus_tag="AOT96_RS19410"
                     /old_locus_tag="AOT96_19425"
     CDS             4010..5680
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003941963.1"
                     /locus_tag="AOT96_RS19410"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AOT96_19425"
                     /product="hypothetical protein"
                     /protein_id="WP_007728318.1"
                     /transl_table=11
                     /translation="MTVVVEAAPPVPASASQFTGTRRLVRLALRRDRIQLSVWVISIVL
                     FMLLVVASVTGLYSTPEELRNIAVSSAVSPVALATNGIVSGDSQGAIVASQSVLVFALV
                     AALMSTLAVVRHTRQNEETGRAEMIGAAVVGRRALLTSALIVTVGANVVLALGIALGSI
                     AVGLPVVGSFALGASIGGTGIAFAGVAAVTAQITDGARTANGLAGAAIGVAFMLRAIGD
                     VNSTVVDDGTRVVSGWLSWLSPIGWAQQIRPFDDDAWWILLLLLGFAAVHVVVAFVLIG
                     HRDQGAGLVQPRPGPPVAASWLPSAWGLAWRMQRMTMFWWIFAVVILGFAYGAVGNEID
                     AMVGSSQQTAEMFEKLGGGGSDLVDSYLATALGLTSIVIAAYSVQSLLRMRGEETSGRL
                     EPLLATSLSRWRWMLSHLGVVVGGVILAHVLMGAFTGFAFGLVTDDIGGNTLRMTGAAL
                     AYLPAVLVVVGLVSLAFGLFPTYASALSWGILAACIVLGQLGTLLKLPQVVLDLSPFTH
                     VPGAPAEPVTVLPLLAMIVVAVVLSTASLVAFRARDLQV"
     gene            complement(5693..6481)
                     /locus_tag="AOT96_RS19415"
                     /old_locus_tag="AOT96_19430"
     CDS             complement(5693..6481)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003941978.1"
                     /locus_tag="AOT96_RS19415"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AOT96_19430"
                     /product="thioesterase family protein"
                     /protein_id="WP_065352149.1"
                     /transl_table=11
                     /translation="MAITSQVSVAAAPSTDAARHHTEFDSSWRSFDGIQGGLAIARMLR
                     VAADASGAEPLAVTAHLDRPVPPGPAELTVGEMRGGRTVSCHVTLTGSGSALVRLARDI
                     GATTMSAPVAEPAPADPSTLPPLEIPVDFVPFAQHLDIRPINSARPFAGGDSPEFDVWI
                     RLLTDLDFTAQERAAVLLDALPPGLFATLTMPVAIPTIEFTAHFTPEHLTADPRTLSPW
                     YRLRHGTAWATDTLCVDESELFDSTGRLAARARQLRRILS"
     misc_feature    6701..6715
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence:
                     medium, score: 20.48"
                     /tool="antismash"
     gene            complement(6715..7191)
                     /locus_tag="AOT96_RS19420"
                     /old_locus_tag="AOT96_19435"
     CDS             complement(6715..7191)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007728315.1"
                     /locus_tag="AOT96_RS19420"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AOT96_19435"
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="WP_050656084.1"
                     /transl_table=11
                     /translation="MNDSAALHKRAVFAEDLEIGQVIPLGSYSVTEEEIVEFATQWDPQ
                     WFHIDKEAAESGVFGGLIASGLHTLSICQKLVVASLYDQWNVIAGKTMRDVSFLRPLRP
                     GDTLSGKVTVDNVVFDNRARGLVTITAKLVDAHGNRVLDVVTDAYLHCRSTRGQ"
     gene            7302..7727
                     /locus_tag="AOT96_RS19425"
                     /old_locus_tag="AOT96_19440"
     CDS             7302..7727
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019744256.1"
                     /locus_tag="AOT96_RS19425"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AOT96_19440"
                     /product="DUF6314 family protein"
                     /protein_id="WP_058038124.1"
                     /transl_table=11
                     /translation="MVDDAKIEPRALVGKWHFERDIVDRLSGSTKHVFGETTISDAGDG
                     LMRWYESGTLRDGDLELPVFRTLFIAERGLEWAVTFEDGREFHDWAPGEEVEHLCGADT
                     YRGRIDVTAGDRSTWTVVWTVTGPSKDYTMTTHLTRP"
     gene            complement(7744..7956)
                     /locus_tag="AOT96_RS19430"
                     /old_locus_tag="AOT96_19445"
     CDS             complement(7744..7956)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005058188.1"
                     /locus_tag="AOT96_RS19430"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AOT96_19445"
                     /product="TOBE domain-containing protein"
                     /protein_id="WP_003942061.1"
                     /transl_table=11
                     /translation="MRLSTRNQLTGTITEVNLGTVMATVKVKLDGGNQIVTSSITRDAA
                     LDLDLEPGQAATVFVKSTEVTIGVE"
     gene            8098..8301
                     /locus_tag="AOT96_RS19435"
                     /old_locus_tag="AOT96_19450"
     CDS             8098..8301
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003942010.1"
                     /locus_tag="AOT96_RS19435"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AOT96_19450"
                     /product="DUF4287 domain-containing protein"
                     /protein_id="WP_007728313.1"
                     /transl_table=11
                     /translation="MTEPVKGPASYFPSIEKKYGRPIGEWQELIRSSDLTKHMELVNWL
                     KSEHGMGHGHANALVAHTLKEG"
     gene            complement(8308..9141)
                     /gene="mftM"
                     /locus_tag="AOT96_RS19440"
                     /old_locus_tag="AOT96_19455"
     CDS             complement(8308..9141)
                     /codon_start=1
                     /gene="mftM"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007728312.1"
                     /locus_tag="AOT96_RS19440"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AOT96_19455"
                     /product="mycofactocin oligosaccharide methyltransferase
                     MftM"
                     /protein_id="WP_065352150.1"
                     /transl_table=11
                     /translation="MSTITNEPVLDSLAPSTPGLWSFGHVTVERVESGPISLDRRRGRL
                     HVKHSLTPADLSERLVAGVTSSIQDAEFGQDEFELTMVGLVRSTVDGALESWTTYYRNS
                     LAELLDGTADFAPIHDKAEELVRGSVLDLGSCFGFLPLRLARAGMDVTATDILPGTMIL
                     LDAVAPELGITIDTVICDAGEVPAPDNSADTVTAIHLLEHVDDAVGEAVIAQALRIARQ
                     RVVIAVPFEDEATACHGHIRTFDLESLRAVGRQSRVPFEVFEHHGGWLVLDASAA"
     gene            complement(9171..9770)
                     /gene="mftR"
                     /locus_tag="AOT96_RS19445"
                     /old_locus_tag="AOT96_19460"
     CDS             complement(9171..9770)
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_function="GO:0036042 - long-chain fatty acyl-CoA
                     binding [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene="mftR"
                     /gene_functions="regulatory (smcogs) SMCOG1215: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007728311.1"
                     /locus_tag="AOT96_RS19445"
                     /note="MftR, the mycofactocin system transcriptional
                     regulator, is an uncharacterized TetR family DNA-binding
                     transcription factor. Its role is inferred by context. It
                     occurs as part of the biosynthesis locus for mycofactocin,
                     a partially characterized electron carrier derived from the
                     terminal Val-Tyr dipeptide of the precursor peptide MftA,
                     through a radical SAM enzyme-mediated process; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /old_locus_tag="AOT96_19460"
                     /product="mycofactocin system transcriptional regulator"
                     /protein_id="WP_065352151.1"
                     /transl_table=11
                     /translation="MRSRKKPSARIGRRPSTTKEGISTIGIELFSTLGFNEVSVDQIAD
                     AAGIARRTFFRYFPSKNAVPWGDFDGHLDEMREHLDSLPADIPLVDALESALLTFNTFP
                     PEETAVHRKRMELILEVPTLQAYSVVMYEGWRQVVADYVARRMNLEPTDHGPRTVGYLL
                     LGVAMSAYEQWLTDDSLELPELLKTGTQSLRTGIST"
     misc_feature    9819..9834
                     /note="TFBS match to CRP, Cyclic AMP receptor protein,
                     confidence: medium, score: 17.13"
                     /tool="antismash"
     misc_feature    9829..9844
                     /note="TFBS match to AfsQ1, Two-component system AfsQ1-Q2,
                     activator of antibiotic production, confidence: medium,
                     score: 17.11"
                     /tool="antismash"
     misc_feature    complement(9921..9937)
                     /note="TFBS match to ZuR_variant_2, Zinc-responsive
                     repressor, confidence: weak, score: 18.73"
                     /tool="antismash"
     gene            9921..10016
                     /gene="mftA"
                     /locus_tag="AOT96_RS19450"
     CDS             9921..10016
                     /GO_function="GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /GO_process="GO:0140604 - mycofactocin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="mftA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005573048.1"
                     /locus_tag="AOT96_RS19450"
                     /note="Mycofactocin is a small molecule electron carrier
                     derived from the final two amino acids, Val-Tyr, of MftA,
                     the mycofactocin precursor. It plays a role in redox
                     homeostasis and the metabolism of alcohols and aldehydes in
                     Actinobacteria, including Mycobacterium tuberculosis;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /product="mycofactocin precursor MftA"
                     /protein_id="WP_063836238.1"
                     /transl_table=11
                     /translation="MSDRDNKALENDLVEESLVEEVSIDGMCGVY"
     gene            10001..10288
                     /gene="mftB"
                     /locus_tag="AOT96_RS19455"
                     /old_locus_tag="AOT96_19465"
     CDS             10001..10288
                     /GO_process="GO:0140604 - mycofactocin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="mftB"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     redox-cofactor: Mycofactocin_RRE"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007728309.1"
                     /locus_tag="AOT96_RS19455"
                     /note="MftB, a small protein, is a peptide chaperone that
                     assists the radical SAM enzyme MftC in performing two
                     modifications to the C-terminal Val-Tyr dipeptide of the
                     mycofactocin precursor peptide, MftA. MftB's role is
                     analogous to the role of PqqD in the biosynthesis of PQQ, a
                     cofactor that derives entirely from a Tyr and a Glu in the
                     precursor PqqA.; MftB, a small protein, is a peptide
                     chaperone that assists the radical SAM enzyme MftC in
                     performing two modifications to the C-terminal Val-Tyr
                     dipeptide of the mycofactocin precursor peptide, MftA.
                     MftB's role is analogous to the role of PqqD in the
                     biosynthesis of PQQ, a cofactor that derives entirely from
                     a Tyr and a Glu in the precursor PqqA; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /old_locus_tag="AOT96_19465"
                     /product="mycofactocin biosynthesis chaperone MftB"
                     /protein_id="WP_007728309.1"
                     /sec_met_domain="Mycofactocin_RRE (E-value: 3.5e-30,
                     bitscore: 102.5, seeds: 14, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRRVLTVSALDLDAGWKLHPQVALRPEPFGALLYHFGTRKLSFLK
                     NTTIVAVVKALPDHPDVRSALRAQGIEDDAAPQYARALSTLADSHMIVPV"
     aSDomain        10037..10276
                     /aSDomain="Mycofactocin_RRE"
                     /aSTool="RREfinder"
                     /database="RREFam.hmm"
                     /description="Stand-alone RRE protein in a mycofactocin
                     cluster"
                     /detection="hmmscan"
                     /domain_id="rrefinder_AOT96_RS19455_Mycofactocin_RRE.1"
                     /evalue="1.60E-32"
                     /identifier="RREFam016.1"
                     /label="AOT96_RS19455"
                     /locus_tag="AOT96_RS19455"
                     /protein_end="92"
                     /protein_start="12"
                     /score="102.5"
                     /tool="antismash"
                     /translation="DAGWKLHPQVALRPEPFGALLYHFGTRKLSFLKNTTIVAVVKALP
                     DHPDVRSALRAQGIEDDAAPQYARALSTLADSHMI"
     gene            10365..11582
                     /gene="mftC"
                     /locus_tag="AOT96_RS19460"
                     /old_locus_tag="AOT96_19470"
     CDS             10365..11582
                     /EC_number="1.3.98.7"
                     /EC_number="4.1.99.26"
                     /GO_function="GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /GO_process="GO:0031179 - peptide modification [Evidence
                     IEA]"
                     /GO_process="GO:0140604 - mycofactocin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="mftC"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     redox-cofactor: TIGR04085"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     redox-cofactor: PF04055"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     redox-cofactor: SPASM"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Fer4_12"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) QueE"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006930977.1"
                     /locus_tag="AOT96_RS19460"
                     /note="MftC is a radical SAM/SPASM enzyme that catalyzes
                     the first two steps in biosynthesis of the electron carrier
                     mycofactocin from the terminal Val-Tyr dipeptide of the
                     precursor peptide MftA.; MftC is a radical SAM/SPASM enzyme
                     that catalyzes the first two steps in biosynthesis of the
                     electron carrier mycofactocin from the terminal Val-Tyr
                     dipeptide of the precursor peptide MftA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /old_locus_tag="AOT96_19470"
                     /product="mycofactocin radical SAM maturase"
                     /protein_id="WP_003942089.1"
                     /sec_met_domain="PF04055 (E-value: 6.3e-23, bitscore: 80.4,
                     seeds: 518, tool: rule-based-clusters)"
                     /sec_met_domain="Fer4_12 (E-value: 2.8e-06, bitscore: 26.1,
                     seeds: 84, tool: rule-based-clusters)"
                     /sec_met_domain="QueE (E-value: 8.1e-10, bitscore: 37.0,
                     seeds: 32, tool: rule-based-clusters)"
                     /sec_met_domain="TIGR04085 (E-value: 1.6e-14, bitscore:
                     52.5, seeds: 281, tool: rule-based-clusters)"
                     /sec_met_domain="SPASM (E-value: 1e-05, bitscore: 24.4,
                     seeds: 166, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTSVLEQPSPPAKVGRLVDQFELGLDAPICLTWELTYACNLSCVH
                     CLSSSGRRDPNELSTEQCKSIIDELQRMQVFYVNIGGGEPTVRSDFWELVDYATDHQVG
                     VKFSTNGVKIDKKVAARLAASDYVDVQISIDGATAEVNDAVRGPGSFAMAERALANLKE
                     AGFKDAKISVVVTRHNVSQLDEFKALADKYDATLRITRLRPSGRGADVWDDLHPRPEQQ
                     RELYNWLVANGEGVLTGDSFFHLSAFGDALPGLNLCGAGRVVCLIDPVGDVYACPFAIH
                     EQFLAGNIVKDGGFQEVWQHSDLFEELRSPQTGGACSKCNHYDSCRGGCMAAKFFTGLP
                     MDGPDPECVIGNGEMALASAGEVPKSSVDHSRSGQRKTPRASVPLTLMMRPPAKICDEN
                     PLAGMQ"
     gene            11638..12816
                     /gene="mftD"
                     /locus_tag="AOT96_RS19465"
                     /old_locus_tag="AOT96_19475"
     CDS             11638..12816
                     /EC_number="1.4.3.26"
                     /GO_process="GO:0140604 - mycofactocin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="mftD"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     redox-cofactor: TIGR03966"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003942047.1"
                     /locus_tag="AOT96_RS19465"
                     /note="MftD, an enzyme found in the mycofactocin
                     biosynthesis locus, performs an oxidative deamination of
                     3-amino-5-[(p-hydroxyphenyl)methyl]-4,
                     4-dimethyl-2-pyrrolidinone (AHDP). The resulting compound,
                     now called pre-mycofactocin (PMFT), is a biologically
                     active redox cofactor that can oxidize the non-exchangeable
                     NADH of TIGR03971 family SDR-type oxidoreductases.; MftD,
                     an enzyme found in the mycofactocin biosynthesis locus,
                     performs an oxidative deamination of
                     3-amino-5-[(p-hydroxyphenyl)methyl]-4,
                     4-dimethyl-2-pyrrolidinone (AHDP). The resulting compound,
                     now called pre-mycofactocin (PMFT), is a biologically
                     active redox cofactor that can oxidize the non-exchangeable
                     NADH of TIGR03971 family SDR-type oxidoreductases.; MftD,
                     an enzyme found in the mycofactocin biosynthesis locus,
                     performs an oxidative deamination of
                     3-amino-5-[(p-hydroxyphenyl)methyl]-4,
                     4-dimethyl-2-pyrrolidinone (AHDP). The resulting compound,
                     now called pre-mycofactocin (PMFT), is a biologically
                     active redox cofactor that can oxidize the non-exchangeable
                     NADH of TIGR03971 family SDR-type oxidoreductases.; MftD,
                     an enzyme found in the mycofactocin biosynthesis locus,
                     performs an oxidative deamination of
                     3-amino-5-[(p-hydroxyphenyl)methyl]-4,
                     4-dimethyl-2-pyrrolidinone (AHDP). The resulting compound,
                     now called pre-mycofactocin (PMFT), is a biologically
                     active redox cofactor that can oxidize the non-exchangeable
                     NADH of TIGR03971 family SDR-type oxidoreductases; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /old_locus_tag="AOT96_19475"
                     /product="pre-mycofactocin synthase MftD"
                     /protein_id="WP_065352152.1"
                     /sec_met_domain="TIGR03966 (E-value: 4.3e-219, bitscore:
                     725.2, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MAKNAFFETVAEAQRRAKKRLPKSVYAALVAGSEKGLTVDDNVAA
                     FSELGFAPHAAGLSDKREMSTTIMGQDISLPVMISPTGVQAVHPDGEVAVARAAAARGT
                     AIGLSSFASKSIEEVAAANPQVFFQMYWVGSRDVLLQRMERARAAGAKGLIITTDWSFS
                     YGRDWGSPSIPEKMDLKAMFQFAPEGIMRPKWLLEFAKTGKIPDLTTPNLAAPGLPAPT
                     FFGAYGEWMQTPLPTWEDIAWLREQWGGPFMLKGIMRIDDAKRAVDAGVSAISVSNHGG
                     NNLDGTPAPIRVLPGIAEAVGDQVEVVLDGGIRRGGDVVKALALGAKAVMLGRAYLWGL
                     SANGQAGVENVLDLMRMGIDSGLMGLGHSSITELSPADLVIPEGFTRTLGAS"
     gene            12843..14774
                     /locus_tag="AOT96_RS19470"
                     /old_locus_tag="AOT96_19480"
     CDS             12843..14774
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Pyr_redox_2"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1217: NADH:flavin oxidoreductase/NADH oxidase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007728304.1"
                     /locus_tag="AOT96_RS19470"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AOT96_19480"
                     /product="mycofactocin system FadH/OYE family
                     oxidoreductase 1"
                     /protein_id="WP_065352153.1"
                     /sec_met_domain="Pyr_redox_2 (E-value: 7.2e-12, bitscore:
                     43.6, seeds: 64, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTLDEHTTLAGRLVRSRVVFGPHETNLAVGRSLSDRHRAYYQRRA
                     EGGTGIVVTEVASVSENDWPYERAPLASQCGPGWQSIAAACAPYGTLVLAGLGHTGMQG
                     SSAYSQSVLWGPSRVADPATRELPMAMGQPEIDQLIVGFREASALAVASGMDGVELDAG
                     PRSILRQFLSGLTNTRQDRYGSDRVALVREVISAVREEVGSDHVVSLRLSCDEEAPWAG
                     LTPDLAIGIAREVSPFLDLLVVVRGGLYSSDRYRPDFHCPPNFNLDLCREIKAALSIPV
                     VVQGSVVDIADAHAALEVGEFVEMTRAQIADPDLVVKAARGDRPRPCVLCNQTCLVRDP
                     RNPVVTCVGNPSAGFETVDPDEREQISTPGAVLVVGGGPAGMEAARISAVAGHSVTLVE
                     SSSQFGGMIRTFARGDGRSRFALLAQWLEDECRRSGVTMRTGAALDAQDLGDARSRGEF
                     VVVATGSAARPPTYPVDDSVRWLDASEAFEKLSTVGPGPVALIDPLGGPIAVALAEALA
                     AHGISVSIVTPDSIVGSRLGASGDLVGANTRIQQAGVTRVLASRVTEIARGAVCVEHIH
                     TGERSQLECEVVIDCSPRLPNETLKEEADIQVGDSVAPRTVLESIREGRFAGFTTRTSF
                     NSAERAIS"
     gene            14771..15598
                     /locus_tag="AOT96_RS19475"
                     /old_locus_tag="AOT96_19485"
     CDS             14771..15598
                     /NRPS_PKS="Domain: PKS_KR (12-187). E-value: 3.5e-15.
                     Score: 48.3. Matches aSDomain:
                     nrpspksdomains_AOT96_RS19475_PKS_KR.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) adh_short"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1001: short-chain dehydrogenase/reductase SDR"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003941966.1"
                     /locus_tag="AOT96_RS19475"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AOT96_19485"
                     /product="mycofactocin-coupled SDR family oxidoreductase"
                     /protein_id="WP_003941966.1"
                     /sec_met_domain="adh_short (E-value: 8.7e-31, bitscore:
                     105.8, seeds: 230, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTQGSVRSLEGRVAFITGAARGQGRAHAVKMAREGAAIIAVDVCA
                     SVASDNSYDAATSEDFAETIRLVEAEGGKILAREVDVRDGAALTAVVKDGVEQFGRLDI
                     VVANAGVCNWNRFWEMSDEQWETLIDINLTGVWKTLKASVPAIIEGGRGGSIIVVSSVA
                     GLKALPGQAHYASAKFGLVGLTQAAAKELGEYKIRVNSIHPYGVNTPMGVDQGALEVFA
                     KFPQYLPNFTPILSDIAFAEPDEIADTVLWLAGDGSRTVTASHIALDQGNSKV"
     aSDomain        14807..15331
                     /aSDomain="PKS_KR"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_AOT96_RS19475_PKS_KR.1"
                     /evalue="3.50E-15"
                     /label="AOT96_RS19475_PKS_KR.1"
                     /locus_tag="AOT96_RS19475"
                     /protein_end="187"
                     /protein_start="12"
                     /score="48.3"
                     /specificity="KR activity: inactive"
                     /specificity="KR stereochemistry: C2"
                     /tool="antismash"
                     /translation="VAFITGAARGQGRAHAVKMAREGAAIIAVDVCASVASDNSYDAAT
                     SEDFAETIRLVEAEGGKILAREVDVRDGAALTAVVKDGVEQFGRLDIVVANAGVCNWNR
                     FWEMSDEQWETLIDINLTGVWKTLKASVPAIIEGGRGGSIIVVSSVAGLKALPGQAHYA
                     SAKFGLVGLTQA"
     CDS_motif       15239..15301
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_AOT96_RS19475_0001"
                     /evalue="1.40E-04"
                     /label="PKSI-KR_m4"
                     /locus_tag="AOT96_RS19475"
                     /protein_end="177"
                     /protein_start="156"
                     /score="14.4"
                     /tool="antismash"
                     /translation="IVVSSVAGLKALPGQAHYASA"
     gene            15598..17547
                     /locus_tag="AOT96_RS19480"
                     /old_locus_tag="AOT96_19490"
     CDS             15598..17547
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Pyr_redox_2"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1217: NADH:flavin oxidoreductase/NADH oxidase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019744250.1"
                     /locus_tag="AOT96_RS19480"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AOT96_19490"
                     /product="mycofactocin system FadH/OYE family
                     oxidoreductase 2"
                     /protein_id="WP_065352154.1"
                     /sec_met_domain="Pyr_redox_2 (E-value: 4.5e-12, bitscore:
                     44.3, seeds: 64, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTPYPRLFAPLRLGNLTLRNRVVFSAHLTNYAENGLPTEQHASYY
                     EARARGGAGLIITEEHSTHPSDRPYEKMIAGYRPEAIEGYRRITDAVHAHGSSILAQIN
                     HNGGQGAGTYSRSPLLAPSAVADPLFREVPKAVDDADIAEILAGFALVARNCVAGGFDG
                     VELQASQSSIVRAFLAPSTNLRTDRYGGGVAHRARFLLEVLGVLRKAIGPDRVLGVRLS
                     GYEGTRGGIELSDAVATAQLIEADGHVDYINTTVGMATETLHLVEASMAVPTGYANFVP
                     SAIREVVDLPVVGVGRFKTPEQAEAALEDGVCDLVGVVRGQIADPDFVNKARAGQSVQV
                     RTCLSCNQECVGRMGLGRWLGCTENPRVGRESVPLPMPRRRGRTVVVVGGGPAGLQAAA
                     TAAQLGHAVTLFERERRLGGQILTASVVPARRELADLVRNLESECRRSGVTIELGAGAD
                     LAIVEERAPEVVVIATGARPLRPAWAGGSERVVGVRDVLDGKILPSGTVLVYDELGFHQ
                     GTSVAEFLADRGCAVTIATNGMVVAQDLSITLDFEGWNRRAFEKSIGQRTDLMVTSVSA
                     GASPLVSLVHLPTGVTEEVAVDWVVFATQQEPVDELWNTLQGSCFEKFRIGDALSPRRS
                     HAAVIEGHRAALAT"
     misc_feature    17571..17586
                     /note="TFBS match to LexA_variant_1, Repressor of DNA
                     damage response, confidence: medium, score: 26.77"
                     /tool="antismash"
     misc_feature    complement(17572..17585)
                     /note="TFBS match to LexA_variant_2, Repressor of DNA
                     damage response, confidence: strong, score: 22.42"
                     /tool="antismash"
     gene            17600..18058
                     /locus_tag="AOT96_RS19485"
                     /old_locus_tag="AOT96_19495"
     CDS             17600..18058
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="AOT96_RS19485"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="AOT96_19495"
                     /product="hypothetical protein"
                     /protein_id="WP_231917231.1"
                     /transl_table=11
                     /translation="MDSRDAWQLDGEGLKGEVLDLVRVRHELQSRMVLLIVEMFSREVL
                     GGRGFRAIAQWLNGSTNLEIGECSLLVGLARLLMLEPLVGDAFHRGDVDALKALQVASF
                     CQHPPMNMTLADVDKARGILLGLASKNIADCDAVRAAIRRIEKAVRQA"
     misc_feature    18146..18159
                     /note="TFBS match to ANR, Anaerobic transcriptional
                     regulator, confidence: medium, score: 21.93"
                     /tool="antismash"
     misc_feature    18243..18256
                     /note="TFBS match to ArgR, Regulator of arginine
                     biosynthesis genes, confidence: weak, score: 17.33"
                     /tool="antismash"
     gene            18297..18989
                     /gene="mftE"
                     /locus_tag="AOT96_RS19490"
                     /old_locus_tag="AOT96_19500"
     CDS             18297..18989
                     /GO_process="GO:0140604 - mycofactocin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="mftE"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019744248.1"
                     /locus_tag="AOT96_RS19490"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AOT96_19500"
                     /product="mycofactocin biosynthesis peptidyl-dipeptidase
                     MftE"
                     /protein_id="WP_042922382.1"
                     /transl_table=11
                     /translation="MSSPRDLAAIPSPALEGTALTVVVPVGSFEQHGPHLPLDTDTRIA
                     RAVAQALPDVVVAPAIEYGASGEHEGFAGTISIGAEAMETVVVEYGRSAFRWTKRVLFV
                     NGHGGNGPALAKAVALLRYEGRDAAWVPCAVAGADAHAGHTETSLLLHLSPDLVHMSLA
                     ETGNLSPIADLMPGMRSGGMVAVSQNGVLGNPVGATAEDGAYIFGELVGRVTDGLARWE
                     PGPDGRLR"
     gene            19087..20484
                     /gene="mftF"
                     /locus_tag="AOT96_RS19495"
                     /old_locus_tag="AOT96_19505"
     CDS             19087..20484
                     /GO_process="GO:0140604 - mycofactocin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="mftF"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Glycos_transf_2"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007730153.1"
                     /locus_tag="AOT96_RS19495"
                     /note="Members of this protein family, MftF, are
                     glycosyltransferases, members of PF00535 (glycosyl
                     transferase family 2). The encoding gene is found as part
                     of the mycofactocin cassette, in Mycobacterium
                     tuberculosis, many other Actinobacteria, and occasional
                     members of other lineages. Mycofactocin itself, a putative
                     redox carrier, is a heavily modified derivative of the
                     C-terminal Val-Tyr dipeptide of the mycofactocin precursor
                     MftA (TIGR03969); Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /old_locus_tag="AOT96_19505"
                     /product="mycofactocin biosynthesis glycosyltransferase
                     MftF"
                     /protein_id="WP_065352876.1"
                     /sec_met_domain="Glycos_transf_2 (E-value: 1.1e-23,
                     bitscore: 82.5, seeds: 256, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRPARLPDGFGIRLDPKVRTYSGGRVLIGGSPTRMLKLAPTAAAM
                     IGDGFLEVVDQQSAAVARHLLDSGVANPRPMSTPSAADVTVVIPVKDNPAGVERLLPVL
                     KDLTVIVVDDGSEVPVEPRRACPGTGTITVVRHESARGPSAARNSGLRSAQTRFVAFLD
                     SDVIPRAGWLELMLGHFSDPGVALVAPRIVALDPYGTALARYENMRSSLDLGRKEAAVK
                     SGSPVAYVPSAAVIVRRDVALECNGFDESLEVAEDVDFCWRLQAAGWRLRYEPVAHVAH
                     DHRVQFDKWFARRAFYGTGAAPLAARHEGSVPPMAMSFSTLFACIAAATLTRSGLAGAL
                     GALLFTVYRLRKMFTGLSQPTRIAAILTAQGVVGGFWQLASAMCRHYWPVTLLAVIASR
                     RIRRLAVAAAVTEGLVDWYRHREPGGLGPVRYVFFKRADDIAYGAGLWRGAFDARDWAA
                     LKPRITK"
     gene            20481..21962
                     /gene="mftG"
                     /locus_tag="AOT96_RS19500"
                     /old_locus_tag="AOT96_19510"
     CDS             20481..21962
                     /EC_number="1.-.-.-"
                     /GO_function="GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA]"
                     /codon_start=1
                     /gene="mftG"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007730154.1"
                     /locus_tag="AOT96_RS19500"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AOT96_19510"
                     /product="mycofactocin system GMC family oxidoreductase
                     MftG"
                     /protein_id="WP_065352155.1"
                     /transl_table=11
                     /translation="MTEADYADFLVVGGGTCGCVVAARLSEDPSATVMLLESGSGYRST
                     LELPDVLGDPYRLPVGPASEYTWTYPVELTPRRASTIARGRTLGGSGAVNGAYFTRATR
                     ADFENWPSSWRYDDVLPYFKKSETDRDFESEFHGTAGPIPVERRAWDQLHPLSGEFHAA
                     ALGAGFPDDVDKNAPDSFGVGRVPLNVSDHRRISTAIGYLMPALHRPNLRVESGVNVIR
                     IAFSGTRAIGVDVLDDGNVRRIRADHVVVCSGAVATPHILLNSGVGPAEQLVEQGVSVV
                     LDRPGVGQNFVDHPEVLLPYHFSKPRTIRSQTPVLETALNLAEIEIRPYTASFTDLVPG
                     VPRMDHGVGVVLMAPRSRGSIELASGDPAGAPRIRYNYVASAHDRAAIREGMRIAENLL
                     ESIAETGLIDRPVMEYTDEWVESRLGTSLHMSGSCVMGAEADPFAVVDGRCRVIGAQGL
                     SIVDTSILPTIPTRGPHATAVMVAERASAILLGDE"
     misc_feature    complement(22023..22038)
                     /note="TFBS match to CRP, Cyclic AMP receptor protein,
                     confidence: strong, score: 18.06"
                     /tool="antismash"
     misc_feature    22340..22350
                     /note="TFBS match to HypR, L-hydroxyproline utilization
                     repressor, confidence: weak, score: 18.79"
                     /tool="antismash"
ORIGIN
        1 gtctgcgcgg tcgcggaagc tgcccgtgcg catctgagtg ccaactggat agtcttcgcc
       61 ttggctgacg gaactttgcc cgacgccgga ccacggcgac tggttctcgg cccggacgcc
      121 accccttttc tggtcgacaa cgtcgagggg ccgccacttc cggaggatgt cgtcgaatgc
      181 ctcgacgaca tccggtacgg aagaatcgaa tccaactcgg tgatcgacgc gcactacgcg
      241 tttgtgccga tcgtgctcgg tggcggcgtg gtaggtgcct ttgcagcctg gacgccggcc
      301 aaccgaaccc tcgacacgac ggacggagcc gtgctcagca ttctggccag ccagactgct
      361 gtggctctgc agaattcggc gctctatcag cgcggacaac tactgctcga gcgtgccgaa
      421 gatgcgtatg cggaggccaa tcgtactgca gccgaccttg cggtgcgcaa cagcgaattg
      481 gagtccactc agcgtcaact cggagtggcg caccggcacc aagtggtcga cgacgaacgt
      541 caccggatcg cccgcgaatt gcacgacagt gtgacacagg cggtgttgtc ggccggcatg
      601 cagatcgagg tctgccggag cgacatcccg gaggctgaac gcagtgaaag actggacttg
      661 gcaaaggatt tgaccaggag ggcggtcgag cagcttcgtt cggcgatcta cgcgctcaat
      721 cattcgtcgg acgcggaccg gacgtcgttg cccgagatgc tcgagcagct cggcgtggtt
      781 cacatgccgg acgagttggc ggtgagcgtt cgcgtcggcg gtaccccgac cgaacttccg
      841 ggcgatgccg aacacgctct tctccggatc gcgggggaag cgctcttcaa tgcagcggtc
      901 catgctcacg cgaatcgggc ggtgcttcgt ctgacctatc gggcggactc cgtgttgctg
      961 tcggtctccg acgacggtga cggcgaccct gcgcgaatgc gtctgatcct caaactcgca
     1021 gccaccaacg acgtcgacgg ccgccatcgc ggtctcgcga acatgcaggc gcgaacactc
     1081 gagttgggtg gtgcgctcga agtacggcgc gcgcggatcg gtgggattca gattctcgtg
     1141 cgactaccac tgccgttcga ctcgggcatg gaaaaccttt gctccaccac tgaaatcgag
     1201 agcgaggaat catgactact gcgcgtcccg tcatcacccg cggagcatcg ccggttctgg
     1261 gattggttcg aatcgtcctg gtcgacgacc atgcaattct gcggcaaggg ctccgctcgg
     1321 ttctcgagcg cgaaagcgat ctcgagatcg tgggggaggc gtcgtccctc gccgaggcac
     1381 tcgccgtggt cgacaaggtc ggcccggaca tcgtcctgat ggatctcaaa ctctccgcca
     1441 gttcggacta cgagggactg accctgtgtg gccagctgtc ctcggcgcat cccggcctcg
     1501 gactgttggt tctcaccacg tttctcgacg atcaactggt ggtgcgagcg gtgcacgcgg
     1561 gtgctcgcgg ctatgtcgtc aaagacgtcg acaccaccga attggtgcgt gcgatcaggg
     1621 cggtctcgcg gggtgagagt gcgttcgatt cgcgcagcgc gtcagctgtc gtgcgatcgc
     1681 tcaacgggca gtcgtcgtcg aaagaacgat tgaccgatcg cgaactcgaa gtgctcagac
     1741 tcctcgcaaa cggcttgtcc aacatcaaga tcggtgagag attgttcatc tcaccgacca
     1801 ccgtcaagtt tcacgtcagc aacatcatgc gcaaactcga ggtgagccgt cgagcagagg
     1861 ccgtctatgc cgcgagtaag caaggtctga tctagctctg ctcggagacc gtccaggcgc
     1921 gcagcgcttg atgctgtagg aggagcacgc tcgaacgagt tgtgcgcaga gtcgacgcgg
     1981 tcatctcggc agtcagaccc actgcgattc gtagtaccag aacctcacgt tggtcgtcgg
     2041 tcaggcgacg cgggcgtttg aattgtccgt cgatgttgcg tgactgggga attgacgcaa
     2101 ttcgtcgaga ggccaactcg tacgcgaagg tctcgatgcg tgtgtcggcc gagatcgtgt
     2161 cggacattcg tgtgacgacg tccagggtgc attggtgggc gagatcgtcg gcgaatgtcg
     2221 cgacgggcgt cccgaggagt ctggcgcgat agtagagaac gacgcgaggg tgcaaccggg
     2281 ccagtagttt cgacagtgca tggtgatcac cggcgaatgc ctcggcggcg gtgcgattca
     2341 gggaaatcga tcgttcacag gtttcgggtg aagcggtacg cgcgaagggt ggagtcggtg
     2401 aagtgttcgc gcccctgatc gacagcttca tcagcaactc ctcgattaca ccgcacctga
     2461 ccatgctgga acgagaattg aggtaccccg ccgaaattgc gggtaaacct gaagttacct
     2521 gccaggtgtc cggtcttttc gccgggagcg ccagtccgcg agcattcgcg gcatttccgc
     2581 ggccatgaag tcgaagaagt cgcgcagatc tgccatacga tcgccggcgg ttgttccttc
     2641 accgaggact cgtatgccct cgtccgcaac tgccgagacc tggtagagcg aggactgttt
     2701 cgtgacaaac gcgtcggccc acatgtcgac gctgacgcag tagtgctgac gccgcgatcc
     2761 ggggacggcc acacgctcga tcaatccgac ttgctggagg tatcggacgg ctccggaaat
     2821 ggcggcgggg ctcactccca accgagatcc gagatctcct ggcgtggacg agggtgtctc
     2881 gctggtcatc aacgcggccc agacgcgggc gggcatcggc ggaaaaccca tgtcgctcag
     2941 tacgagtgag agtcgctcga catagtgtcg gctctcgtcc ggtgcaacgg tgtggctggt
     3001 gtcggtcatc ggaacccttc gatctttccg tcttttctat cttcacacat ttgtgaaatc
     3061 ggcgtacggt caaattcatg ggtgcggcaa tttcagtgaa aagcctcgtc aagaccttcg
     3121 gtaccacccg cgcgctggac ggcctcgacc tcgacgtctc gacgggtgaa gtgcacggat
     3181 tcctggggcc gaacggttcc ggaaagtcca ccactattcg ggtgctcctc ggcctgctcc
     3241 gctcggactc cggagacgca accgttctcg gcggcgatcc gtggcgagac gctgttactt
     3301 tgcatcgacg tctcgcctat gtgcctggcg acgtgaatct gtggcccaac ctcaccggcg
     3361 gtgaggctat cgatctgttg gcgggcctgc gcggtggctt ggaccgaaaa cgccgcagcg
     3421 agttgctcga tcgcttcgaa ttggacccga ccaagaaggc gcgtacgtac tcgaaaggta
     3481 accggcagaa ggttgcactc gtggccgcgt tctcgtccga cgtcgacctt tacgtcctcg
     3541 acgaacccac atcggggctc gacccgttga tggaggcggt attccaggac tgcgtcgagg
     3601 aggtggctca cgccggaaag acggtactgc tgtcgagcca tatcctcgcc gaggtcgaag
     3661 cgctgtgcga tcgtgtgagc atcatccgcg acgggcggac ggtggaagag ggaacactgt
     3721 ccgaactcag gcatctcacg cgcacgtccg tcgcggcgga gacgcagcgt ccgatcggtg
     3781 gactcgacgg actcggcggt gtccacgacg cggtgatcga cggtaaccac gtcacctgcg
     3841 aagtcgacag cgcgcaactc ggaccactca tgacgtactt ggtcggattc ggaatcgtgg
     3901 cgctcacttc cactccgccg actctcgaag aacttttctt gcggcattac ggtgacgaac
     3961 gggccaggga gacttcggta cacctgcgag ccgagaccgg agtacggccg tgaccgttgt
     4021 tgtcgaggcc gctccaccgg taccggcctc ggcaagtcaa tttaccggca cacgccgact
     4081 ggttcggctg gctttgcggc gggaccgaat tcagctctcc gtgtgggtta tctccatcgt
     4141 cttgttcatg ctgctggttg tcgccagtgt gaccgggctg tactcgacac ccgaggaatt
     4201 acgaaacatc gcagtcagtt ccgcggtatc gccggtcgcg ctggcgacca acgggatcgt
     4261 gtccggtgat tcgcagggcg cgatagtcgc cagccagtcg gtgctcgtgt tcgcgttggt
     4321 ggccgcattg atgagcaccc tcgcggtggt tcgccatacg cgacagaacg aggagaccgg
     4381 ccgagccgag atgatcggtg cagcggtggt aggtagacga gcgctcctga cctccgcact
     4441 gatcgtaacg gtcggtgcga acgtcgttct ggcactggga attgcactgg gatcgatcgc
     4501 cgtcggtttg cctgtcgtcg gttcgttcgc gttgggtgcc tcgataggtg ggaccggaat
     4561 cgcgtttgcc ggagtggcag ccgtgacggc gcagatcacc gacggtgctc gtacggccaa
     4621 cggcctggcg ggtgccgcga tcggcgttgc cttcatgctg cgggcgatcg gcgacgtgaa
     4681 cagtacggtc gtcgacgacg gaactcgcgt ggtcagtggc tggttgtcgt ggctctcgcc
     4741 gatcgggtgg gcacaacaga tccgaccctt cgacgacgac gcctggtgga tcctgttgtt
     4801 gctgctcgga tttgcggctg tgcacgtggt ggtggctttt gttctcatcg gccatcgcga
     4861 tcagggagcg ggactcgttc agccccgacc cggcccgcca gtcgcggcat cgtggctacc
     4921 gtcggcttgg ggcctggcgt ggcgcatgca gcgaatgacg atgttctggt ggatctttgc
     4981 cgttgtcata ctcgggttcg cgtacggcgc tgtgggcaac gagatcgacg cgatggtggg
     5041 atcgtcgcag cagaccgcgg agatgttcga gaaacttggc ggcggcggat cggatctggt
     5101 ggattcctac ttggccactg ctctggggct cacgtcgata gtcatcgcgg cgtattcggt
     5161 ccagagtctt ctccggatgc ggggcgagga aacatcggga cggctcgaac cgttgctggc
     5221 aacgtctttg tcgcggtgga ggtggatgct ttcccacctg ggtgtggttg tgggcggcgt
     5281 gatcctcgcg cacgtcttga tgggtgcctt caccggtttt gccttcgggc tcgtcacgga
     5341 cgacatcggc ggcaacacac ttcgcatgac tggcgccgcg ttggcctatc tgcctgcggt
     5401 gctcgtagtc gtggggttgg tgtcgttggc tttcggactg tttccgacgt acgcgtcggc
     5461 gctctcctgg ggaatcttgg cggcatgcat cgtgctcggg caactcggca cgttgctgaa
     5521 gcttccgcag gtagtgctgg atctgtcgcc cttcacccac gtgccgggtg cccctgccga
     5581 gccggtgaca gttctgccac tgctggcaat gatcgttgtg gcagtggtgc tttcgacagc
     5641 atcgcttgtt gcgttccgtg ctcgcgacct ccaggtgtag gagagctgaa actcaactca
     5701 gaattcgacg aagctggcga gcccgagcag caagtcgtcc ggtcgagtcg aatagttcgg
     5761 attcgtcaac acagagagtg tccgtcgccc aggcagtccc gtggcgtagg cgataccacg
     5821 gtgacagcgt tcggggatct gccgtgaggt gctccggggt gaaatgcgcg gtgaactcga
     5881 tggtcggtat ggccacgggc atcgtcagtg tcgcgaacag tccgggcgga agcgcgtcga
     5941 gaaggaccgc ggcacgctct tgggccgtga aatcgaggtc tgtgagcagg cggatccaga
     6001 cgtcgaattc aggggagtcg ccaccggcga acggcctcgc ggaattgatc ggccggatgt
     6061 cgaggtgctg ggcgaacggc acaaaatcca ccgggatctc cagcggcggc agggttgacg
     6121 ggtcggccgg agccggttcg gcgacagggg cggacattgt tgtagctcct atgtcgcggg
     6181 ccaaccgaac gagagcggat cccgaaccgg tcagcgtgac gtggcaactg acggtccgcc
     6241 cgccccgcat ctcgccgaca gtcaactcgg ccggcccagg aggaacgggg cggtccaggt
     6301 gcgccgtgac tgccagcggt tcggctccgg acgcgtcggc cgccactcgc agcatccgtg
     6361 caattgcgag tccgccctgg atgccgtcga aactccgcca actggagtcg aattcggtgt
     6421 gatgacgcgc tgcgtcggtc gacggcgcgg ccgccactga tacctgacta gtaattgcca
     6481 tagtcagcac cctagatgct gactatggct atggcaagct agggtctatt cgggagtgct
     6541 ggacgatcca ccgaagagca tgtagatgag gatctgaaga atgccgagag gattgatctc
     6601 gccgaggctg ctcatgtgtg aggaactcct gattgcaggg atgggtattg ttgcgtcgca
     6661 acgtcactga aagtacggca attcggacag tgtggtgcga aatttaccga taattcactg
     6721 tccgcgagtc gagcggcagt gcaggtaggc gtcagtgacc acgtcgagaa cgcgattgcc
     6781 atgcgcatcg accaatttcg ctgtgatggt caccaatcct cgcgcgcgat tgtcgaacac
     6841 gacgttgtcg acggtgacct ttccgctcag ggtgtcaccg gggcgaagcg gacggaggaa
     6901 gctcacgtcg cgcatggttt tcccggcgat cacgttccac tggtcgtaga gactggcgac
     6961 caccaacttc tggcagatcg acagggtgtg cagcccgctg gcgatcaacc cgccgaagac
     7021 gccggactcg gcggcttcct tgtcgatgtg aaaccactgg ggatcccact gagttgcgaa
     7081 ctcgacgatt tcctcttcgg tcaccgaata cgaacccaat gggatcactt ggccgatctc
     7141 gagatcctcg gcgaacactg cgcgtttgtg aagggcggcc gaatcgttca tggttgtgaa
     7201 aatctcctgc gtgttccggg tgtactcgga acatactctc gcagtgtgtt cgagtcggtc
     7261 tccggtgtcg tcgggcaatg cagggtcact agaatcgcga catggtcgac gacgcgaaaa
     7321 tcgagccgag ggcgctggta ggaaagtggc acttcgagcg ggacatcgtc gaccgccttt
     7381 ccggttccac caagcatgta ttcggcgaga ccactatctc cgacgccggt gacggtctga
     7441 tgcgctggta cgagagcggc acgctgcgtg acggcgacct ggaattaccc gtgttccgaa
     7501 ctctgttcat tgccgagcgc ggcctcgagt gggccgtgac attcgaagac ggcagagagt
     7561 tccacgactg ggcgccgggg gaggaagtcg agcacctgtg cggtgcggat acctaccgcg
     7621 ggcgaatcga cgtcactgcc ggagatcgat cgacctggac tgtcgtctgg accgtgacgg
     7681 ggccgagcaa ggactacacg atgaccacgc atctcacccg gccctgatca catcgctcgc
     7741 cagttactcg acgccgatcg tcacctcggt ggacttcacg aataccgttg cggcttggcc
     7801 tggttcaaga tcgagatcca gtgcggcatc gcgggtgatc gacgaggtca caatctgatt
     7861 cccgccgtcc aacttcacct tgaccgtcgc catgacggtg ccgaggttga cctcggtgat
     7921 ggtgccggtg agttgatttc gtgtggacag tcgcatcgct gaactcctgt gctctcgggt
     7981 gggtgttctt cgaccataat gcgatggtcg cgtgtctacc cagcgattcc gcaggtgcgg
     8041 acatgcgggc gccgccggtg cgcgaatatt gtcgggcggt ggaggtagat tccggtcatg
     8101 actgaacccg tgaaaggccc ggcgtcgtat tttccgtcga tcgagaagaa gtacggtcgg
     8161 ccgatcggtg aatggcaaga gctgatcaga agctcagacc tcacgaagca catggaattg
     8221 gtcaactggt tgaagtcgga gcacggtatg ggtcatgggc acgcgaatgc gcttgtcgcc
     8281 catacactga aagaaggcta gttcttttca ggcggccgat gcgtccagca ccaaccagcc
     8341 gccgtgatgc tcgaatacct cgaacggcac acggctctgc cggccgaccg cacgcagtga
     8401 ctcgagatcg aaagtgcgga tgtggccgtg gcacgcggtg gcttcgtctt cgaaggggac
     8461 tgcaatgacg acccgttgac gcgcgatccg gagtgcttgc gcgatcacgg cctcgccgac
     8521 tgcgtcgtcg acgtgttcga ggagatgaat cgcggtgacg gtgtcggcgc tgttgtccgg
     8581 tgccggtact tcacccgcgt cgcagatcac cgtgtcgatc gtgatgccga gttcgggggc
     8641 aaccgcgtcg agcaggatca tcgttccggg caggatgtcc gtggcggtca cgtccattcc
     8701 ggcgcgcgcg agccgcaggg gcaggaaacc gaagcaggaa cccagatcga gcacggaacc
     8761 gcggaccagc tcttcggctt tgtcgtgaat cggcgcgaaa tctgcagtgc cgtccagtag
     8821 ttcggcaagg gagttccggt agtaggtggt ccaggattcg agagcgccgt ccacggtgga
     8881 gcgaaccagg ccgaccatcg tcaactcgaa ttcgtcctga ccgaactccg cgtcctggat
     8941 cgacgaggtg actccggcga cgagtcgctc actcagatcg gcgggagtga gggagtgctt
     9001 gacgtggagg cgaccgcgtc ggcgatcgag cgagatcgga ccggactcga cgcgttcgac
     9061 ggtgacatgc ccgaacgacc acaagcccgg cgtcgacggc gccagggaat ccaggacggg
     9121 ttcgttcgtg atggtggaca tgatcgcctt tctcaggtgg tgatgctttc tcaggtgctg
     9181 ataccggtcc gcagggactg cgtacctgtt ttcaagagtt ccggtagctc gagtgaatcg
     9241 tcggtgagcc actgctcgta cgccgacatc gcgaccccga gcagaagata gccgaccgtg
     9301 cgcgggccgt gatcggtcgg ctcgagattc attcggcgtg cgacgtagtc ggcgaccacc
     9361 tgccgccaac cttcgtacat caccacagag tatgcctgaa gcgtcggaac ttcgaggatc
     9421 aattccatcc gtttgcgatg taccgcagtc tcttccggtg ggaaggtgtt gaacgtcaac
     9481 aatgcggatt cgagtgcgtc gaccaacgga atatcagcgg gcagtgaatc caggtgctcg
     9541 cgcatctcgt ccagatgtcc gtcgaagtct ccccagggga cggcgttctt ggacgggaag
     9601 tagcgaaaga aggtccgacg cgcgatgccg gcggcgtcgg ctatctggtc gacgctcacc
     9661 tcgttgaagc cgagtgtcga gaacagttcg atcccgatcg tgctgattcc ctccttcgtt
     9721 gtggaggggc gacgacctat ccgagcggac ggtttcttgc ggctacgcat acttttttcg
     9781 ctccatgtgt gtcattctgc actcgatgcc attacagtgg tgaaccgagt cacagtgaag
     9841 tcccactagt gaccaccaac tcccaggttg gaagtctgat ctacagactt ccagacggag
     9901 tgcgaccgca ggaggaatcc atgtcagatc gtgacaacaa ggcgctcgag aacgacctcg
     9961 tcgaggaatc cctcgtggag gaagtttcca tcgacggcat gtgcggcgtg tactgaccgt
    10021 gtctgctttg gatctcgatg cggggtggaa actccacccg caggtagcgc ttcgccccga
    10081 acctttcggg gcgttgctct accacttcgg aacacgcaag ctgtcgttcc tgaagaacac
    10141 gaccatcgtt gcggtggtca aggcgttgcc agaccatcct gatgtccggt ccgcactacg
    10201 tgcgcaaggc atcgaagatg acgcggctcc ccaatatgcc cgcgcgctga gcactctcgc
    10261 cgactcgcac atgatcgtgc ctgtctgagc cagacgctcg cccccacgtg cggaacagcc
    10321 cttccgcacg ttcgcccgct tcatttcttc gcgaggacac agccatgacc tcagttcttg
    10381 aacagccctc cccgcccgcc aaggtgggtc gcctcgtcga tcagttcgaa ctcggactcg
    10441 acgcccccat ctgcctgacg tgggaactca catacgcctg caacctgtcc tgcgtgcact
    10501 gcctttcgtc gtcgggtcgt cgtgacccca acgagctgag caccgaacag tgcaagtcca
    10561 tcatcgacga gctgcagcgt atgcaggttt tctacgtcaa catcggtggc ggcgagccga
    10621 cggtacgctc cgacttctgg gagctcgtcg actacgcgac cgaccaccag gttggtgtga
    10681 agttctccac caacggcgtg aagatcgaca agaaggtcgc ggcgcgcctc gcagccagtg
    10741 actacgtcga cgtgcagatt tccatcgacg gtgccaccgc tgaggtcaat gacgctgtgc
    10801 gcgggcctgg ttcgttcgcg atggccgagc gcgctctcgc gaacctgaag gaagccggct
    10861 tcaaggacgc caagatttcg gtggtcgtca cgcggcacaa cgtcagtcag ctcgacgagt
    10921 tcaaggctct cgcggacaag tacgacgcga ccctgcgcat cactcgactg cgtccttcgg
    10981 gccgcggcgc cgacgtgtgg gacgacctgc acccgcgtcc ggagcagcag cgtgagctct
    11041 acaactggct ggtcgccaat ggcgaaggcg tcctgacggg cgactcgttc ttccacctct
    11101 ccgccttcgg cgatgcactg cccggtctca atctctgtgg cgcgggccgc gtcgtgtgcc
    11161 tgatcgaccc ggtcggtgac gtgtacgcat gccccttcgc gattcacgag cagttcctcg
    11221 ccggaaacat cgtgaaagac ggtggattcc aggaagtctg gcagcactcg gacctgttcg
    11281 aggagctgcg atcgccccaa accggtggcg cctgcagcaa gtgcaaccac tacgactcct
    11341 gccgcggcgg ctgcatggcg gccaagttct tcaccggact gccgatggac ggacctgacc
    11401 ccgagtgcgt catcggcaac ggtgaaatgg cactggcgtc ggcgggagag gtcccgaagt
    11461 ccagtgtcga ccactcccgt tccggtcaac gtaagacccc acgtgcatcg gtgccactga
    11521 cactgatgat gcgtccgccc gccaagattt gtgacgaaaa cccgcttgcg ggaatgcagt
    11581 aactactgcg cgataccgac agtgcggcga aagctgcacc ccagagaggt cagttcaatg
    11641 gctaagaatg ctttcttcga gacggtggca gaagcacagc gccgtgcgaa gaagaggttg
    11701 cccaagtccg tgtacgcggc gttggtagcg ggatccgaga aagggctgac ggtcgacgac
    11761 aacgtcgcgg cattcagcga attgggtttc gctccccacg ctgccgggct gtcggacaag
    11821 cgcgagatgt ccaccaccat tatgggccag gacatttcgt tgcccgtcat gatttcaccg
    11881 accggtgtgc aggcagtgca ccccgacggt gaagtagcgg tagcccgtgc ggctgcagca
    11941 cggggaacag ccatcggact gagttctttt gccagcaagt ccatcgaaga agttgccgcg
    12001 gccaacccgc aggtcttctt ccagatgtat tgggtcggca gccgcgacgt gctcctgcag
    12061 cgtatggagc gcgctcgcgc tgccggtgcc aagggcctga tcatcaccac cgactggtcc
    12121 ttctcgtacg gtcgcgactg gggcagcccg tccatccccg aaaagatgga cctcaaggcg
    12181 atgttccagt tcgcacccga gggaatcatg cgcccgaagt ggttgctcga gttcgccaag
    12241 accggcaaga tcccggacct gaccaccccc aacctcgcgg ctcccggcct gcctgcaccg
    12301 acgttcttcg gcgcatacgg cgagtggatg cagactccgc ttccgacgtg ggaagacatc
    12361 gcctggcttc gtgagcagtg gggtggaccg ttcatgctca agggcatcat gcgcatcgac
    12421 gacgccaagc gagctgtcga cgccggtgtc tccgctatct cggtctccaa ccacggtggt
    12481 aacaacctcg acggcacccc ggcgccgatc cgtgtgctcc cgggcattgc cgaggccgtg
    12541 ggcgatcagg tggaagttgt tctcgacggc ggcattcgac gcggcggcga cgtcgtcaag
    12601 gcgctcgcac tcggagccaa ggccgtcatg ctcgggcgcg cctacctgtg gggcctctcg
    12661 gccaacggcc aggccggtgt cgagaacgtg ctcgacctca tgcggatggg catcgactcg
    12721 gggctgatgg gcctcggcca ctcgtccatc accgaactga gcccggcgga cctggtgatt
    12781 ccggaaggct tcacacgcac attgggtgcc agctgagatt acggctccga tagagctgtg
    12841 agttgacact cgacgaacac acgaccctgg ccggacgcct cgtccggtcc agggttgttt
    12901 tcggtccgca cgagactaat cttgctgtgg gacgctcgct ttccgatcgg caccgtgcgt
    12961 actaccagcg ccgcgccgag ggcggaaccg ggatcgtcgt caccgaagtg gcctcggtat
    13021 cggagaacga ctggccgtac gagcgggcgc cgttggcgtc gcaatgtggc ccggggtggc
    13081 agtcgatcgc ggctgcatgt gctccgtacg gaacgctggt tctggccggg ctcggtcata
    13141 ccggcatgca gggatcgagc gcatactccc agtcggtgct gtggggaccg tcaagagtgg
    13201 ccgacccggc gacgcgtgag ttgccgatgg caatggggca gccggaaatc gaccagctga
    13261 tagtcggctt ccgtgaggcg tcggcgctgg ccgtcgcttc gggaatggac ggagtggaac
    13321 tcgatgccgg tcctcgaagc attctgcgac aattcctttc gggtctgacc aacactcggc
    13381 aagaccgata cggtagcgat cgcgttgctc ttgttcgcga ggtcatctcg gcagtgcgcg
    13441 aggaagtcgg gtcggatcac gttgtctccc tgcggttgtc ctgtgacgag gaagctccat
    13501 gggcgggtct gacgcccgat cttgccatcg gtatcgcgcg tgaagtgtct ccgttcctcg
    13561 atctccttgt cgtcgttcgt ggcggactct attcgtctga ccggtaccgc cccgattttc
    13621 actgcccgcc gaacttcaac ctcgatctgt gccgtgagat caaggccgct ctgtcgattc
    13681 ctgttgtcgt gcaggggagc gtcgtggaca tcgccgacgc tcacgcggcg ctggaagtag
    13741 gtgagttcgt cgagatgacc cgggcccaga tcgcagaccc ggacctggtt gtgaaagccg
    13801 cgcgcggcga tcgtccccgg ccgtgtgtgc tctgcaatca gacgtgcctc gtgcgtgatc
    13861 ctcgcaatcc tgtcgtgacg tgcgtcggga atccgagcgc gggtttcgag acggtggacc
    13921 ccgatgagcg agagcagatt tcgacaccgg gagccgtgct ggtcgtgggg ggtggccccg
    13981 ctgggatgga agccgcacgg atctcggcgg tggcggggca ctcggtcaca ctcgtcgaga
    14041 gttcatcgca gttcggcggc atgatccgaa cttttgcgcg aggtgacggc cgtagccggt
    14101 tcgcactcct cgcgcagtgg ttggaggacg aatgccgtcg tagcggcgtg acgatgcgga
    14161 ccggggcggc gctcgacgcc caagacctgg gtgacgcacg cagccggggc gagtttgttg
    14221 tcgttgccac cggtagtgct gcgaggccgc cgacgtatcc ggtcgacgat tcggttcgat
    14281 ggttggatgc gtcggaagcc ttcgagaagt tgagcaccgt ggggccgggt ccggtcgcgc
    14341 tgatcgatcc cctcggtgga ccgatcgcgg tggcgctcgc agaagcactc gcggcccatg
    14401 gcatctccgt ctcgattgtg actcccgaca gcatcgtcgg ttcgaggctc ggtgcgagtg
    14461 gggatctggt cggcgccaac acgcgcatcc agcaggccgg ggtcaccagg gtgctcgcgt
    14521 cacgtgtcac cgaaatcgct cgaggggccg tctgcgtcga acacattcat accggcgagc
    14581 ggtcacagct cgagtgcgag gtggtgatcg actgctcgcc tcgtctgccg aacgaaacgc
    14641 tgaaggaaga ggccgacatt caggtgggcg actcggttgc cccgagaact gttctcgaat
    14701 ccatccgcga agggcgcttt gcagggttta ctactagaac gagtttcaat tctgcagaga
    14761 gggctatctc atgacacagg gttcagttcg ttcgctcgaa ggtcgcgttg ctttcatcac
    14821 cggcgcagcg cgcggtcaag gacgtgcgca cgcggtcaag atggcgcgcg agggagcagc
    14881 gatcatcgcg gtcgacgtat gcgcgtcggt ggcgtcggac aattcctacg acgcagcaac
    14941 ttccgaggac tttgccgaga cgatacgtct ggtggaagcg gagggcggga agatcctcgc
    15001 ccgtgaggtc gatgtccgtg acggcgcagc gctcaccgcg gtggtcaagg acggcgtcga
    15061 acagttcgga cgtctcgaca tcgtggtcgc caacgccggg gtctgcaact ggaatcgatt
    15121 ctgggaaatg tcggacgagc agtgggaaac cctgatcgac atcaacctga ccggagtctg
    15181 gaaaaccctc aaggcctcgg tgccggcgat catcgagggt gggcgcggtg gatcgatcat
    15241 cgtggtcagt tcggtggccg gactcaaggc gctgcccgga caggcgcact acgcgagcgc
    15301 caagttcggt ctcgtcggtc tgacgcaggc cgcggcaaag gaactggggg agtacaagat
    15361 cagggtcaac tcgatccatc cttacggagt gaatacgccg atgggggtcg accagggcgc
    15421 tctcgaggta tttgcaaagt ttccgcagta ccttcccaac ttcactccga tcctctcgga
    15481 catcgctttc gccgaacctg acgagatcgc cgataccgtc ctgtggctcg ccggtgacgg
    15541 ttcccgcacc gtcacggcaa gtcatatcgc tctcgatcaa ggaaactcca aggtctgatg
    15601 acgccgtacc cgcgcctctt cgcgccactg cgcctgggca acctgacact ccgcaatcgg
    15661 gtcgtgttct ccgcacacct gaccaattac gcggagaacg ggttgccgac ggagcagcac
    15721 gcgagctact acgaggcgcg ggcacgcggc ggtgcgggtc tgatcatcac cgaggagcac
    15781 tcgacgcatc cttcggatcg accgtacgag aagatgattg cgggctatcg gccggaagcg
    15841 atcgagggat accggcggat caccgacgcc gttcatgcgc atggttcgtc gattctcgcg
    15901 cagatcaacc acaatggggg tcagggcgct ggaacctatt cgcgatcgcc gctgttggcg
    15961 ccgagcgccg ttgcggatcc gttgttccgc gaggtgccca aggctgtcga cgacgcggac
    16021 attgccgaga ttctcgcagg gttcgccctt gttgcgcgca actgcgttgc cggtggcttc
    16081 gacggggtgg aattgcaggc gtcgcagtcg tccatagtgc gcgcgtttct cgccccctcg
    16141 accaacctgc gcactgatcg ctacggcggg ggcgtggcac atcgtgcacg gttccttctc
    16201 gaagtgctcg gggtactgcg taaggccatc ggcccggatc gtgtcctcgg tgtgcgcctg
    16261 agtggatacg aaggaacgcg gggtggcatc gaactgtccg acgcggtagc caccgctcaa
    16321 ctcatcgaag ctgacggtca cgtcgactac atcaatacca ccgtcggaat ggccacggaa
    16381 acactgcatc tggtcgaagc atcgatggct gtacccaccg gctacgccaa cttcgtcccg
    16441 tctgcgatca gggaggtcgt cgatcttcca gtcgtcggag tcggccgttt caagacgccg
    16501 gagcaagccg aagccgcact cgaggacggt gtatgcgatc tggtgggagt agttcgcggg
    16561 cagatcgccg atccggattt tgtgaacaag gcgcgtgctg gtcaatcggt acaggtgcga
    16621 acctgcctgt cctgcaacca ggaatgcgtc gggcggatgg gtctgggacg ttggttgggg
    16681 tgcaccgaaa atccgcgcgt cggtcgggaa tcggtaccgc tgccgatgcc gcggcgacgg
    16741 ggccgcacgg tggtggtggt cggtggcggc cccgccggcc tgcaagctgc ggctacagcg
    16801 gcccagctag gtcatgcggt gacgctgttc gagcgtgaac gaagacttgg gggacagatt
    16861 cttaccgcct cggtggtacc ggctcgccgt gagctcgccg acctcgtccg gaatctcgaa
    16921 tctgagtgtc gccgaagcgg tgtgacgatc gagctgggtg ctggagctga tctcgcgatc
    16981 gtggaggagc gcgcgcccga ggtggtcgtg attgcaaccg gagcgcggcc tttacgccca
    17041 gcgtgggcgg gtggatcaga gcgtgtggtg ggtgttcgag acgttctcga cggcaagatt
    17101 ctgccgagtg gaacggtgct cgtctacgac gaactgggat tccatcaagg tacgtcggtg
    17161 gctgagttcc tcgctgatcg aggctgtgcc gtcacgatcg cgaccaacgg catggtggtc
    17221 gcgcaggacc tgagcatcac cctggatttc gaggggtgga accgccgggc gttcgagaag
    17281 tcgatcggtc aacggacaga tctgatggtg acttcggtga gtgctggggc ctcgccgctc
    17341 gtgagcttgg ttcaccttcc gacgggtgtg accgaggaag ttgcagtcga ctgggtggtc
    17401 ttcgcgacac agcaggaacc tgtcgacgag ttgtggaaca ccttgcaggg aagctgtttc
    17461 gagaaatttc gcatcggtga cgccttgtct ccgaggcgtt cgcacgcggc cgtcatcgag
    17521 gggcatcggg ccgcgttggc gacataagtc gtccggtttg tctctggtta tcgaaagtat
    17581 gttcgattaa actttgagca tggacagtcg ggatgcgtgg cagctggatg gtgagggcct
    17641 caagggtgag gttctggatc tggtgcgcgt ccggcacgag ttgcagtcgc ggatggtgtt
    17701 gttgatcgtg gagatgtttt cccgggaggt tctcggcggg aggggttttc gggcgatcgc
    17761 gcagtggttg aatggttcga cgaatttgga gatcggtgag tgcagtctgt tggtcgggtt
    17821 ggcgcggttg ttgatgctcg aacctcttgt cggcgatgcg tttcaccgcg gtgacgttga
    17881 tgcgttgaaa gcccttcagg tggcgtcgtt ttgtcagcat ccgccgatga acatgaccct
    17941 ggcggatgtg gacaaggctc gcgggattct gttggggttg gcgtcgaaga acatcgctga
    18001 ttgtgatgcg gtgcgggcgg cgatccggcg gatcgagaaa gcagtacggc aagcgtgagg
    18061 acggcgttcc ggtgggtgca ccacaccgag tggcgggtgg agatcggccc ggatcggaaa
    18121 acggtgttct atccaccgat gtcgatcgat ccgtatcaac agccgatcgg cgggaacagc
    18181 ccaccgacag cggcgtgaac cgcgacaact gacgaatacg ccggggcaca acaatgcccc
    18241 ggtgtatccg catgcagtgt tgcggtggga ggggcccgca ccaatacgat cgggggatga
    18301 gttctccgcg tgaccttgct gccatcccga gtcctgcgct cgagggcact gcgctcaccg
    18361 tcgtggtccc ggtcggttct ttcgaacaac acggaccgca cctgcctctc gataccgaca
    18421 ctcggatcgc gagagcagtc gcacaggctc tgcccgacgt cgtcgtggcc ccggccatcg
    18481 aatacggggc gagcggcgaa cacgaagggt ttgccggcac gatttcgatc ggtgccgagg
    18541 ccatggaaac cgtcgtcgtc gaatacggaa ggtcggcgtt ccgatggacg aagcgggtgc
    18601 tgttcgtcaa cggccacggc ggaaatggtc ccgcattggc caaagcggtg gcacttctgc
    18661 ggtatgaggg gcgtgatgct gcttgggtgc cgtgcgctgt tgcgggtgcc gacgctcatg
    18721 ccggacacac tgaaacgagc ctattgctgc acctttcgcc cgatttggtg cacatgagcc
    18781 ttgctgagac aggaaatttg tctcctatcg cggacctgat gccaggaatg cgttcgggcg
    18841 gtatggtcgc tgtgtcgcaa aacggcgttc tcggcaatcc agtcggggct accgccgaag
    18901 acggcgcata catcttcggt gagttggtag gacgcgtgac ggatggactg gcccggtggg
    18961 agcctggtcc agatgggagg cttcggtgac gaccacgacc gagtgggggc ggacggtcca
    19021 cctgcgacgg ctttcgaggc tgcgacgagt tgcagcacga cgggctacgt cccttacgat
    19081 catctgatgc gaccagcaag actccccgac ggcttcggca ttcggttgga ccccaaggtg
    19141 cgcacgtact cgggtggccg ggtactcatc ggcggatctc cgacgcggat gctcaagctg
    19201 gcgccgacgg ctgcggcgat gatcggtgac ggcttcctcg aggtggtcga tcagcagagc
    19261 gctgcggttg cgcgacacct tctcgattca ggtgtcgcga atcctcgtcc gatgtcgacg
    19321 ccgtccgctg ccgatgtcac ggtcgtgatt ccggtcaagg acaacccggc cggcgtcgaa
    19381 cgcctattgc ccgtcctcaa ggatctgacc gtcatcgttg tcgacgacgg ttcggaagtg
    19441 ccggtggagc cccgtcgcgc ctgcccgggc accggcacca tcacagtggt ccggcacgag
    19501 agtgcccgcg gaccctcggc agcgagaaac tcgggattgc gttcggccca aacacgtttc
    19561 gtagcttttc tggactccga cgtcataccg cgtgccggtt ggctcgagtt gatgttgggc
    19621 cacttcagcg atccgggggt tgcccttgtt gcaccccgca tcgtcgccct cgatccgtac
    19681 gggacggcgc tggcgcggta cgagaacatg cgatcgtctc tcgacctcgg acgtaaggaa
    19741 gctgcggtga agtcgggcag tccggtcgcc tatgtcccca gcgccgccgt gatcgtccgg
    19801 cgcgacgtgg cactcgaatg caatggtttc gacgagagtc tcgaagtagc cgaggacgtc
    19861 gatttctgct ggcgactgca ggctgcgggt tggcgactga ggtacgaacc ggttgcgcat
    19921 gtcgcccacg atcatcgagt tcagttcgac aagtggttcg ccaggagagc gttctacggc
    19981 acgggtgcgg cgccgttggc tgcaagacac gagggttcgg ttccgccgat ggcgatgtcg
    20041 ttctcgacgc tcttcgcgtg tatcgccgct gcaacactga cccgttccgg tcttgccggc
    20101 gcactcggcg ctttgctgtt taccgtttac cggctgcgaa agatgttcac cggcctgagt
    20161 caacccaccc ggatcgcggc gatcctcacc gcgcagggag ttgtcggcgg attctggcaa
    20221 ctcgcctcgg caatgtgtcg tcactactgg cccgtgacgc tcctggcggt gatcgcctcc
    20281 aggcgaattc ggcggctggc cgtcgctgca gcagtcaccg aaggtttggt ggactggtat
    20341 cgccaccgtg agccaggcgg gctgggaccg gttcggtacg tcttcttcaa acgagccgac
    20401 gacatcgcgt atggagccgg actctggcgt ggcgccttcg acgcccgcga ctgggcggcg
    20461 ctgaaacctc gaatcaccaa gtgacagaag ccgactacgc agatttcctg gttgtcggtg
    20521 gtggcacgtg tggttgcgtg gtcgcagcca ggctcagtga agatccatcc gccacggtga
    20581 tgctcctcga atcgggaagc ggatatcgat cgacgctcga actcccggac gtcctgggtg
    20641 atccgtaccg actgccggtc ggacctgcga gcgaatacac gtggacctat ccggtcgaac
    20701 tgacgccgcg tcgggcgtcc acaattgcgc gggggcggac actcggtgga tccggagccg
    20761 tcaacggtgc gtacttcacc cgcgcgaccc gtgctgattt cgagaattgg ccgagttcat
    20821 ggcgatacga cgacgtcttg ccgtacttca agaagtccga aacggatcgt gatttcgaga
    20881 gcgagttcca cgggaccgcc ggaccgatcc cggtcgagcg tcgagcgtgg gaccaactgc
    20941 atccactgag cggcgagttt catgcggcag cgctcggagc aggctttccc gacgacgtcg
    21001 acaagaatgc gccggactcg ttcggggtgg gccgagtgcc actgaacgtg tcggatcatc
    21061 gtcggatcag tacggcgatc gggtatctga tgccggcgct gcaccggccg aatctgcggg
    21121 tggaatccgg agtgaacgtc attcgtatcg cgttctcggg tacccgtgcg atcggcgtcg
    21181 acgttctcga cgacggcaat gtccgacgca ttcgtgccga ccatgtggtc gtgtgttcgg
    21241 gtgcggtggc gacaccgcac attctcctca attccggtgt tggtccagcg gaacaacttg
    21301 tagagcaagg agtttccgtc gttctcgatc gaccgggcgt cggacagaac ttcgtcgacc
    21361 acccggaagt gctgctgccg taccattttt cgaagccccg cacgattcga tcgcagactc
    21421 cggtgctgga gacagccctc aacctcgctg agatcgaaat ccgtccgtac accgcatctt
    21481 tcacagacct ggttcccggt gtgcctcgca tggaccacgg cgtcggagtg gtcctcatgg
    21541 cgccgcgcag ccggggaagc atcgagctgg catcggggga tcccgctggg gcgccgcgta
    21601 ttcggtacaa ctacgtcgcg tcggcgcacg atcgagctgc cattcgggaa gggatgcgga
    21661 tcgccgagaa tcttctcgag tcgatcgccg agacggggtt gatcgatcgc cccgtcatgg
    21721 agtacaccga tgagtgggtc gaatcccgcc tgggaacgtc gctgcatatg tccggtagtt
    21781 gcgtgatggg tgccgaggcg gacccgttcg cagtcgtcga cggtcggtgc cgtgtgatcg
    21841 gcgcgcaagg actcagtatt gttgacacgt caatcctgcc gacgatcccg acccgcggcc
    21901 cgcatgcgac cgcggtgatg gtcgcggaac gagcaagcgc cattctgctc ggcgacgagt
    21961 gaccgcgagg ggcagcaggc tgggtcggca gtcataccga atggtcgttc tcgcctatgg
    22021 gctgtgatcg gcgttacagt ggtccacgga gcggaggaac gcaaaatatg gcacgtgaac
    22081 aggctgtgcc cggcaacccg tgggtggcgg tcaggccagg cgatgacgtc gcgttactcg
    22141 cgcggcgact ttcgtctgcc catcagtcgt tcatcgatgc ccgcggcgtg cccggccagg
    22201 ggctgaccga cgactcccat gtccgctcgg tcgtactcga gtcctggatg cgtagccgca
    22261 acaagggggt aaatcccgat gtggtcggca accctgagat gctcgaaggg ttggagctcg
    22321 aaaaatatcg ctccgcgcat cccatgtcac tgatccgtcc ggtgattcgg aaactcctgg
    22381 tggaagatgc tgccgagacc ggactactga tcgcgatcag tgacgcacac ggtcgattgc
    22441 tctgggtgga gggtgacaac tcggccaagg acaaggcgtt gtccatgaac ttcgtcgaag
    22501 gcgccgattg gagcgaagat cgtgtcggca ccaatgcgcc gggaacggcg cttgctctcg
    22561 atcactccgt acagattttc ggatccgagc atttcaaccg aaccgtgcac gactggagtt
    22621 gttcggccgc acccgtccac gatcccacca ctgggcaaat ccttggtgcc atcgacatca
    22681 ccggtggtcc cagggttgcg gttcccgaag tgctgtcact gatccgtgca actgtcgcgg
    22741 ccgccgaatc ggaattgcga ttccatctct tgaactcgcc catcccactc agtgccacgg
    22801 ctccgcgcct cgaaac
//
