LOCUS       NZ_CP012749            10888 bp    DNA     circular CON 05-MAR-2024
DEFINITION  Rhodococcus sp. 008 chromosome, complete genome.
ACCESSION   NZ_CP012749
VERSION     NZ_CP012749.1
KEYWORDS    RefSeq.
SOURCE      Rhodococcus sp. 008
  ORGANISM  Rhodococcus sp. 008
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Nocardiaceae; Rhodococcus.
REFERENCE   1  (bases 1 to 6570200)
  AUTHORS   Loewen,P.C. and Brassinga,K.
  TITLE     Genome sequence of a ureolytic Rhodococcus species isolated from
            skate fish
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6570200)
  AUTHORS   Loewen,P.C. and Brassinga,A.K.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-SEP-2015) Microbiology, University Manitoba, 418
            Buller Bdg, Winnipeg, Manitoba R3T 2N2, Canada
REFERENCE   3  (bases 1 to 6570200)
  AUTHORS   Loewen,P.C. and Brassinga,K.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-SEP-2015) Microbiology, University of Manitoba, 408
            Buller Building, Winnipeg, Manitoba R3T 2N2, Canada
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: smrtanalysis v. 2.3.0.140936
            Genome Coverage                   :: 176x
            Sequencing Technology             :: PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 03/04/2024 12:54:36
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,967
            CDSs (total)                      :: 6,896
            Genes (coding)                    :: 6,809
            CDSs (with protein)               :: 6,809
            Genes (RNA)                       :: 71
            rRNAs                             :: 5, 5, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 5, 5, 5 (5S, 16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 87
            CDSs (without protein)            :: 87
            Pseudo Genes (ambiguous residues) :: 0 of 87
            Pseudo Genes (frameshifted)       :: 36 of 87
            Pseudo Genes (incomplete)         :: 58 of 87
            Pseudo Genes (internal stop)      :: 13 of 87
            Pseudo Genes (multiple problems)  :: 17 of 87
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 16:00:06
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 1514920
            Orig. end                         :: 1525808
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP012749.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Source bacteria are available from Dr. A. K. Brassinga, Dept of
            Microbiology, University of Manitoba, Winnipeg, MB R3T 2N2, Canada.
            This genome has a base modification file available.
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10888
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="butyrolactone"
                     /region_number="1"
                     /rules="AfsA"
                     /tool="antismash"
     cand_cluster    1..10888
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="AfsA"
                     /kind="single"
                     /product="butyrolactone"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10888
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5888](+)"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5888
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
     gene            49..918
                     /locus_tag="AOT96_RS07195"
                     /old_locus_tag="AOT96_07185"
     CDS             49..918
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1039: aldo/keto reductase family oxidoreductase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /locus_tag="AOT96_RS07195"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AOT96_07185"
                     /product="aldo/keto reductase"
                     /protein_id="WP_197487714.1"
                     /transl_table=11
                     /translation="MTVAPKTFDDGPIQGLGLMRLTDSGWGDADRDPAALVRAAIDSGV
                     PMLDTAEMYGNEELVGRAIGTNREHITLCSKFGVYRGPSGRPDDWNVRADSATVRAAID
                     GSLRRLDVDHLDLYYLHHRSEDTPIEETVNAMAELRQAGKIRALGLSNVTLDDIRRAHA
                     VHPITAVQEQWSLIRRDVELFLPTLADLGITLVAHSPMGHGDLNHSTGNLVARSLASIA
                     SRVGATAGQVALAWVHQRGRGVAATVIPLPGTTSIAHLQANIAASRIDLSETDLADLDD
                     VTQQTREQ"
     gene            1041..1793
                     /locus_tag="AOT96_RS07200"
                     /old_locus_tag="AOT96_07190"
     CDS             1041..1793
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) APH"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020111094.1"
                     /locus_tag="AOT96_RS07200"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AOT96_07190"
                     /product="phosphotransferase"
                     /protein_id="WP_042925138.1"
                     /sec_met_domain="APH (E-value: 3.1e-16, bitscore: 58.5,
                     seeds: 156, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTEVEVVVAHTERATLRVGEVFLKIDADQSNTDVEVEAMLRAPIP
                     TAQILWRTPPVLALAALPGHALGRLGRPSTTSSAAWAAAGAAVRTLHDAPLPPWPGRSL
                     DDIASELDRECAWLTRSGVVATGVITSNRRIAEIALRSWTPVFTHGDLQASHVFVTDDE
                     VAGVIDWSEAAQGDPLYDLATLTLGHPEHLDDVVEGYGTDVDLEVIQAWWSLRSLRGVR
                     WLVEHGFDPAAPGCEVDVLNRQAMRDRQ"
     gene            complement(2157..3656)
                     /locus_tag="AOT96_RS07205"
                     /old_locus_tag="AOT96_07195"
     CDS             complement(2157..3656)
                     /EC_number="1.14.13.-"
                     /GO_function="GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /GO_function="GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Pyr_redox_2"
                     /gene_functions="other (smcogs) SMCOG1092: hypothetical
                     protein"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019745606.1"
                     /locus_tag="AOT96_RS07205"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AOT96_07195"
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="WP_063315606.1"
                     /sec_met_domain="Pyr_redox_2 (E-value: 2.6e-08, bitscore:
                     31.9, seeds: 64, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTQHVDVLIIGAGLSGIGAACHLIREQTGSTYAILERRENIGGTW
                     DLFKYPGIRSDSDMLTFGFGFRPWIGTKVLADGASIRDYVEETAKEYGVTDHINFGRKV
                     VAMDFDRATAQWSVTVLVEATGETETWTANVLVGACGYYNYDKGYRPAFPGEDDFRGQI
                     VHPQHWPEDLDYTGKKVVVIGSGATAITLIPSMAPTAGHVTMLQRSPTWIQALPSEDPV
                     AKGLKLARVPDQIAYKIGRARNIALQRASFQLSRTNPKLAKKLFLTQIRLQLGKNVDLR
                     HFTPNYNPWDQRLCVVPNGDLFKVLKSGKADIVTDRIATFTEKGIVTESGREIEADVIV
                     TATGLNVQILGGATMSIDGEPVKLNETVAYKSVLYSDIPNFLMILGYTNASWTLKADLA
                     ASYLCRVLKIMRKRDYTTFEVHAEPEDFAEESLMGGALTSGYIQRGDGEMPRQGARGAW
                     KVVNNYYRDRKLMHDAEIEDGVLQFSKVDIPVVPDSKVASA"
     gene            4173..4832
                     /locus_tag="AOT96_RS07210"
                     /old_locus_tag="AOT96_07200"
     CDS             4173..4832
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007729739.1"
                     /locus_tag="AOT96_RS07210"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AOT96_07200"
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="WP_047271918.1"
                     /transl_table=11
                     /translation="MTLSSTVTVYTTHSNRGFANAIAAGISARAYSEPQTADMSLLRHH
                     TSDHGRALVLLPLGGVDWQVKELSALEGSVLVGDLSSGNSMIRGLAAGAHAVLNSQLPF
                     TSLLDELQAAINPNSARSAHDKSSLVQRIRLRTEEFARISSLTDREFEIVGLLYGGSNV
                     DSIARTAHIAPTTVRAHIRSILQKLGVHSQLEACAMLRRSGHDARRHPLELRIGRF"
     gene            5001..5888
                     /locus_tag="AOT96_RS07215"
                     /old_locus_tag="AOT96_07205"
     CDS             5001..5888
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     butyrolactone: AfsA"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007729740.1"
                     /locus_tag="AOT96_RS07215"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AOT96_07205"
                     /product="ScbA/BarX family gamma-butyrolactone biosynthesis
                     protein"
                     /protein_id="WP_065351495.1"
                     /sec_met_domain="AfsA (E-value: 6e-18, bitscore: 63.2,
                     seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MEYTRTVPRHLVHRHAVAEVFLTDYRIESDSAYQVAVQLPVSHRI
                     FDVSSEQHDPLGVAEAFRQAVVLLCHTRYEIPLHYKFVMETFSFEMLDALDVQSVAEPL
                     SFSMTVDNLTHRDGAVSGVEVSGILSEGNRHVARCNAVARGVSPEGYRRIRQGREDHRP
                     SRRILPSGAAVVGSERVGRLTDGDVLISTDPARHAMFCAPDPRNHSLFDHPVDHIPGMI
                     LFEAARQALRFHIGNPATKIASLSAVFPRFTEWETPCEVSTIPLDASGHVHRVRFTQEL
                     ETTAELEITVADQS"
     gene            complement(5927..6571)
                     /locus_tag="AOT96_RS07220"
                     /old_locus_tag="AOT96_07210"
     CDS             complement(5927..6571)
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1057: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007729741.1"
                     /locus_tag="AOT96_RS07220"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AOT96_07210"
                     /product="ScbR family autoregulator-binding transcription
                     factor"
                     /protein_id="WP_003940238.1"
                     /transl_table=11
                     /translation="MQERAVLTRQAVILGAAKSFEKFGYSASLGTILQHGGVSKGAMYF
                     HFASKEELAHAVIAAQHGMAMEGTRRVAAHSDIAVETLVLVSQEMARQLVTEPIARGGM
                     RLTMEIGSIQGPIEQPYLDWIEAIKQVATQAAEAGDIVPGTDIDALARFVVGSFTGVQI
                     LSEMLTGRTDLYERLSQMWNLVLPTIVREDKLAHVMQLAALAPREWEAPSA"
     gene            6658..7500
                     /locus_tag="AOT96_RS07225"
                     /old_locus_tag="AOT96_07215"
     CDS             6658..7500
                     /GO_function="GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /NRPS_PKS="Domain: NAD_binding_4 (78-162). E-value:
                     6.6e-07. Score: 20.6. Matches aSDomain:
                     nrpspksdomains_AOT96_RS07225_NAD_binding_4.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) RmlD_sub_bind"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Polysacc_synt_2"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1010: NAD-dependent epimerase/dehydratase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003940604.1"
                     /locus_tag="AOT96_RS07225"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AOT96_07215"
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="WP_065351496.1"
                     /sec_met_domain="RmlD_sub_bind (E-value: 3.4e-12, bitscore:
                     44.5, seeds: 37, tool: rule-based-clusters)"
                     /sec_met_domain="Polysacc_synt_2 (E-value: 2e-06, bitscore:
                     25.6, seeds: 77, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTTLVLGATGFIGSAVMKQLVSRHGNAAIGFVRTATAAKQFGREG
                     IATVIGDINESASLRVAMSQASTVICCVSYVGDDEQRCVHVNQHGVQNVASMAATVGVE
                     RLLYVSTAAVYGPGPFRDLPVNGAPLSPFSPASRTRALAEKFVRDAGGLVVRPHLVYGP
                     GDRWFLPTLTRIVSRLDSIIDGGSAVLSVVDVDLLARSIYELSIEQQFRYGSTLHVNEP
                     VPRTVIDLLEQHARETNWTLPQNSIPRADAVKAAAQLSLDMHQIDMISLDHWFRSRLY"
     aSDomain        6892..7143
                     /aSDomain="NAD_binding_4"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_AOT96_RS07225_NAD_binding_4.1"
                     /evalue="6.60E-07"
                     /label="AOT96_RS07225_NAD_binding_4.1"
                     /locus_tag="AOT96_RS07225"
                     /protein_end="162"
                     /protein_start="78"
                     /score="20.6"
                     /tool="antismash"
                     /translation="DEQRCVHVNQHGVQNVASMAATVGVERLLYVSTAAVYGPGPFRDL
                     PVNGAPLSPFSPASRTRALAEKFVRDAGGLVVRPHLVYG"
     gene            7859..8977
                     /locus_tag="AOT96_RS07230"
                     /old_locus_tag="AOT96_07220"
     CDS             7859..8977
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019745611.1"
                     /locus_tag="AOT96_RS07230"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AOT96_07220"
                     /product="hypothetical protein"
                     /protein_id="WP_065352781.1"
                     /transl_table=11
                     /translation="MLTADILLSGPRGRRMLLAYAIDAERALRPEPGDDMFRINAHRAA
                     YHLDIGQGTSQVLFGPGAEEARQTIVTAEGVARSLTEISLPDVTAVDLRSALAEAVDAA
                     RYWQDPDGEDVLVATEVMRDELRRVAEHIAGSECARWWSTSLASSDQWSILSRGEESPD
                     TDEVPAAQQLLSEWRTSTLESEARAERDRPVDPAANWSGYWWSNPPTQGSTRALFDGTP
                     AGLWFVEDSMGWERVVVRALRPPTGARIYEIDSAQAWSELCRRFPLEVTAETRQVWYRT
                     TGRSGGWVIPDWVQVAERFEGVHLTVTGYLAAAGTAIEVDTETASVIAGWAPDETYWFT
                     DAATYISEPCAWVLHDGPEVTDQPAWTLESQQ"
     misc_feature    9145..9160
                     /note="TFBS match to CopR, Copper-responsive regulator,
                     confidence: weak, score: 21.62"
                     /tool="antismash"
     gene            9197..9910
                     /locus_tag="AOT96_RS07235"
                     /old_locus_tag="AOT96_07225"
     CDS             9197..9910
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1071: GntR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /locus_tag="AOT96_RS07235"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AOT96_07225"
                     /product="FCD domain-containing protein"
                     /protein_id="WP_065351497.1"
                     /transl_table=11
                     /translation="MPDSRIAPTARNIPLSVQIAEQLRSQITSGNWELGEKIPGEHQLV
                     EMTGASRNTVREAIRGLVHAGLLEARPGDGTYVCASSELEIALQRRAAAENVLEIFEAR
                     EALELYAARLAAERALPEDIDRMTRALDGRDAEPDVAKSVEYDLDFHLEMFAASGNQLI
                     TDIYRSLDRSDTMSIPQGKSDKETRQLFIGPWTDDDPHRQLLAAIGDRDPDGAATAVVR
                     LMDHSRSLFLQNMPK"
ORIGIN
        1 tctttggcga cctggttctg atcagcggcc ccgataatgt actcattcat gactgtcgcg
       61 ccgaaaacat tcgatgacgg gcccatccag ggcctgggcc tgatgcgatt gaccgacagc
      121 gggtggggtg atgctgaccg cgaccccgcc gcactggttc gcgccgcgat cgactccggg
      181 gtgcccatgc tcgacacagc cgagatgtat ggaaacgaag agctggttgg tcgggcaatt
      241 ggcaccaacc gtgaacacat cacgttgtgc agtaaattcg gtgtctaccg cgggccgtcc
      301 ggtcgtcctg atgactggaa tgttcgggcg gactctgcaa cggtgcgcgc ggccatcgac
      361 ggctcactca ggcgcctcga cgtcgatcac ctcgatcttt actacctgca tcaccgcagc
      421 gaggacacac caatcgagga gaccgtcaac gctatggccg agctccgcca ggcagggaag
      481 atccgggctc tggggctgtc caatgtcacg ctcgatgaca tccgacgagc tcatgccgta
      541 cacccgatca ctgccgtgca agagcaatgg tcccttatac ggcgagatgt tgaactgttc
      601 ctcccaacgc tcgccgacct cgggatcaca ctggtcgcac attcgccgat ggggcacggc
      661 gacctcaacc attccaccgg caatcttgtc gctcggtccc ttgccagcat cgcttctcgt
      721 gtcggagcga cggctggcca ggtcgctctt gcgtgggtcc atcagcgcgg gcgcggggtc
      781 gcggccaccg tcattcccct gcccggcacc acgagcatcg ctcatctaca agccaacatt
      841 gctgcttcgc gaattgatct cagtgagact gacttggcgg accttgatga tgtcacgcag
      901 caaacaaggg agcagtaagg cagtttgttg agaaagtttg aggtgcggga ggtcagcgga
      961 ccataccgcg ctccgatcgc gaatgaccgc acgcgagaaa cccggcgacg ccaaggcgca
     1021 tcccagccat actgaccgac gtgacagaag tcgaggtcgt cgtcgcccat accgagcgcg
     1081 cgactctgcg cgtcggtgag gtgttcctca agatcgacgc cgatcagtcg aataccgacg
     1141 tcgaggtcga agcaatgctc cgggccccaa tcccgacagc gcagattctg tggcgcacac
     1201 cgcccgtgct cgcgctcgcc gctctgccgg gacacgcgct cggtcgccta ggcagaccgt
     1261 cgaccacgtc ttcagcggcg tgggccgcag cgggtgccgc cgttcgaacg ctgcacgacg
     1321 caccgttgcc gccctggccc ggccgtagtc tcgacgacat cgcctcagaa ctggaccgcg
     1381 aatgtgcatg gctgaccagg agcggggtgg ttgccaccgg agtgattaca tccaaccgtc
     1441 ggattgccga gattgcgctc aggtcgtgga caccggtatt cacgcacggc gatctccaag
     1501 cctcccatgt gttcgtgacc gatgacgaag ttgccggcgt catcgactgg tccgaagccg
     1561 ctcagggcga tcccttgtac gacctcgcaa cactgaccct cggccacccg gaacatctcg
     1621 atgacgtggt tgagggctac ggcactgacg tcgacctcga ggtcatccag gcgtggtggt
     1681 cgttgcgcag cttgagaggc gtgcgctggc tggtcgagca tggtttcgat ccagccgcgc
     1741 cgggctgcga agtagacgtg ctgaatcgac aagcgatgcg cgaccgacaa taaccgaacc
     1801 atcaggacgt tctccccgat ctgccaaacc ttcgactcgc gccaaaattg ctgcagccaa
     1861 aagcatttcg tgtcgatgac ggcacggtta ggcaggtcgc gacgcgttcg cgccgccgtt
     1921 gattgccgtc agccggcaaa cgcctggata ttgtcagcga gcggtggtcg tctcaatcga
     1981 gcttcggacc gcggcaagca tgcccagccg cccagatggc agaccgaagt ctggactcgc
     2041 tcttcctgca gcaactttcg aaacgccacg aacgcgaacc tcgactctcc gacaaaacga
     2101 aaagatcacg gattcaggca agggggcgca accccgttca ttcgagtgga gccggctcag
     2161 gcgctggcga ccttgctatc aggcacgaca ggaatgtcta ccttgctgaa ctgcagcaca
     2221 ccgtcttcga tctcggcgtc gtgcatcagc ttgcggtcgc ggtagtaatt gttgaccact
     2281 ttccacgcgc cgcgggcacc ctgacgcggc atttctccgt cgccgcgctg gatgtagccc
     2341 gaggtcaggg ctccgcccat gagagattct tccgcgaagt cctcaggttc ggcgtgaacc
     2401 tcgaaagtcg tgtagtcgcg cttacgcatg atcttgagta cgcgacacag ataggacgcg
     2461 gccaggtcgg ccttgagcgt ccaggatgcg ttggtgtagc cgaggatcat caggaagttc
     2521 ggaatgtcgg agtagagcac gctcttgtag gcgaccgtct cgttgagctt gacgggctcg
     2581 ccgtcgatgc tcatggtcgc cccgcccagg atctgtacgt tcaagccggt cgccgtgacg
     2641 atgacgtcgg cctcgatttc gcggcccgac tcggtcacga tgcccttctc ggtgaacgtg
     2701 gcgatacggt cggtgacgat gtcggccttg ccactcttga gcaccttgaa caggtccccg
     2761 ttgggaacca cgcacaggcg ctgatcccac gggttgtagt tgggggtgaa gtgacgcagg
     2821 tccacgttct tgccgagttg caagcggatc tgggtgagga acagcttctt cgccagcttc
     2881 ggattggtgc gtgagagctg aaagctggcg cgttgcagtg cgatattgcg ggctcgaccg
     2941 atcttgtaag cgatctgatc gggaacgcgt gcaagcttga gacccttggc cacagggtcc
     3001 tcggacggta gcgcctggat ccacgtgggc gagcgctgca gcatggtgac gtgaccggca
     3061 gtgggggcca tcgatgggat cagcgtgatc gcggtggcac cggaaccgat gaccactacc
     3121 ttctttccgg tgtaatcgag atcctccggc cagtgctgcg ggtgcacgat ctgaccgcgg
     3181 aagtcgtcct caccggggaa ggcggggcgg tatcccttgt cgtagttgta ataaccacag
     3241 gcgccgacga ggacgttcgc ggtccacgtc tcggtctccc ccgtcgcctc gaccaggacg
     3301 gtcacggacc actgtgcagt cgcgcgatcg aagtccattg ccacgacctt gcggccgaag
     3361 ttgatgtggt cggtgacgcc gtattccttg gcggtttcct cgacgtagtc acggatactg
     3421 gcgccgtctg cgagcacttt ggttccgatc cacggacgga aaccgaatcc gaaggtgagc
     3481 atgtcggaat ccgaacggat gccgggatac ttgaacaggt cccaggtgcc accgatgttc
     3541 tcgcggcgtt cgaggatcgc gtaagtgctt ccggtctgct cacgaatgag gtggcaagcc
     3601 gcgccgattc cggacaagcc agcgccgatg atcagtacgt cgacatgctg tgtcatgagg
     3661 aaactcgttt cgtcggtcgt cgcgaagcta cttctggcgt cggcagtgac cgagaacgat
     3721 cggccggttc aggccgggtt ccctaaggtc agcgccaatg aacagggtat gtgcaactga
     3781 cggtaacagt caatatctcg acccggccgc gcacggaaca ttgaggcgcg acgcaccggc
     3841 ctcgcggaac tcgatcgaga cgcctgaatg cctcgagaca ccggagagtt gttcacgtcg
     3901 tcgacggaac gcccaatgac tcgtctacac gggatgctca tcgcccaaat attcagcgcc
     3961 ccagcataat tcgaaggtcc atgatcaggt gacgaacgcg cgagtttcca tgggaagacg
     4021 ggcaactgcg atatcgccgt aaccttcgtg cgcatcagcg gaaaggtcac tgcgatagct
     4081 aacctgctga cgaaaccacg ctgaaagcaa cggttttggt ttcgcagcga agagagcggg
     4141 cggagcggac ctccaccgga gatgtagtcg ctatgacact ctccagcaca gtcaccgtgt
     4201 acaccactca tagcaatcga gggttcgcca atgcaatcgc tgccggaatc agcgccaggg
     4261 cttattccga gccacaaacc gcagacatga gcctgttaag gcatcacacc agcgatcacg
     4321 gtagagcgct cgttctccta ccactcgggg gcgtcgattg gcaggtgaag gagctctccg
     4381 cgctcgaggg atcggtcctg gtcggcgatc tcagtagcgg caactccatg attcgggggc
     4441 tcgcagcggg cgcccacgcc gtgctcaact ctcaactgcc attcacatcc ctgctcgatg
     4501 agctgcaggc cgcgatcaac cccaactccg ctcggtctgc acacgacaag tcctcgctcg
     4561 tacaacgcat ccggttgcgc accgaagaat tcgcacgaat cagcagtctc accgatcgcg
     4621 agttcgagat cgtgggcctt ctctacggag gctcgaatgt cgactcgatt gctcgaaccg
     4681 cccacatcgc accgaccacg gttcgcgccc acatccgaag catcctgcaa aaactggggg
     4741 tgcattccca gctggaggca tgcgcgatgc tccgccgctc tggtcacgac gcacgcagac
     4801 acccactgga acttcggatc ggacgcttct agcaggaagt gccccagaca cactcgccga
     4861 gatggggtgc cgcccctgat cgaactcgac cttcgaaaag atcgtcaatt ttgacgattc
     4921 gatgacgcgc ccggacgtcg aatattaggt cctacacgac accgaagaca cgagtgaaca
     4981 tcgaaaggtg cattcaatca atggaataca cacgcacagt gccgcggcat ctcgtccaca
     5041 gacatgctgt tgccgaggtt ttcttgacgg actaccggat cgaatcggac tcggcgtacc
     5101 aggttgctgt gcaactcccg gtctcgcacc ggatattcga cgtatcgagc gagcaacacg
     5161 atccgctcgg ggtggcagaa gcatttcgac aggcagtggt gcttctgtgc cacacccgat
     5221 acgagattcc actgcactac aagttcgtca tggaaacgtt cagcttcgag atgctggacg
     5281 cgctcgacgt gcaatcagtc gcggagccgt tgagtttctc gatgacagtg gacaacctca
     5341 ctcatcgcga cggagcggtt tcgggggtcg aagtgtcggg gatcctcagc gagggcaacc
     5401 ggcacgtcgc cagatgcaac gctgttgcgc gaggagtcag ccccgaggga taccgtcgaa
     5461 ttcggcaagg acgagaagac caccgcccga gccgacgcat ccttccttcc ggagccgcgg
     5521 tggtcggatc ggagcgagtc ggacgcctaa ccgatggaga tgtgctcatc agcactgatc
     5581 ctgctcggca cgcgatgttc tgcgctcccg atccgcgcaa ccactccctt ttcgaccacc
     5641 ccgtcgacca catcccagga atgattcttt tcgaggccgc ccgacaagcg ctccgatttc
     5701 acatcggcaa cccagcaaca aagatcgcct cactctcggc ggtctttccg aggttcactg
     5761 aatgggaaac gccgtgcgag gtgtcaacga tcccccttga cgcgtcgggc catgtgcacc
     5821 gagttcggtt cactcaagaa ctggaaacca ccgcggaact cgaaattaca gttgcggacc
     5881 aatcatgaga gggaacgaac acgttcttct cgtcgcgtct accacgtcaa gcgctcggtg
     5941 cttcccactc tctcggtgca agcgccgcca gttgcatgac gtgcgccaac ttgtcctcgc
     6001 ggacgatcgt tggcagcacc aggttccaca tctgactcaa ccgctcgtac agatcggttc
     6061 tgcccgtcag catttccgag agaatctgca ctccggtgaa cgaccccacc acaaatcgag
     6121 ccaacgcgtc tatatcggtg cccgggacga tatcgccggc ttcggccgct tgggttgcca
     6181 cctgcttgat agcctcgatc caatcgaggt acggctgttc gatggggcct tgaatactgc
     6241 cgatctccat cgtcaaccgc atgccacctc gcgcgatggg ctcggtcaca agctgccgag
     6301 ccatctcctg cgaaacgagc accagggttt cgaccgcgat gtcggagtgc gcagcaaccc
     6361 gccgggtacc ctccatcgcc ataccgtgct gcgctgcgat gaccgcgtgc gcaagctcct
     6421 ccttggacgc gaaatgaaaa tacatcgccc ccttcgacac acccccgtgc tgcaagatcg
     6481 tgcccaaact ggcggagtac ccgaacttct cgaagctctt cgcagcaccc aaaatgacgg
     6541 cctggcgcgt caggacagca cgctcctgca tgatctaccc agctctcgtt gccgacggaa
     6601 tgaatctcaa cgatacgtcg aaagagcccg cagcaacgct ctcgacacgg acacaccgtg
     6661 acgactctgg tactcggggc cactggtttc atcggctcgg cagtgatgaa acagctggtt
     6721 tcccgccacg gaaacgctgc aatcggtttc gttcgcacgg ccaccgcagc caaacagttc
     6781 ggccgtgaag gaatcgccac cgtgatcggc gacatcaacg aaagtgcttc tctacgagtt
     6841 gcaatgagtc aggcgtcgac cgtcatctgt tgcgtgtcgt acgtgggcga cgacgaacaa
     6901 cggtgcgttc acgtcaacca acacggcgta cagaacgttg cgtcgatggc cgccaccgtc
     6961 ggcgtcgaac gcttgctcta cgtcagtacc gcggccgtgt acgggccggg accgtttcga
     7021 gacttgccgg tcaacggcgc acctctcagc cccttctcac ctgccagtcg cacgcgcgct
     7081 ctcgccgaga agttcgtgcg cgacgccgga ggcctggtcg tccggccgca tctcgtgtac
     7141 ggacccggcg acagatggtt tcttccgaca ctgacgcgaa ttgtctctcg cctcgactcg
     7201 atcatcgacg gcggtagcgc tgtcctgtcg gtagtcgacg tagacctact cgcccgatcc
     7261 atctacgagt tgtcgataga acagcaattt cgctacggct caacacttca cgtcaacgag
     7321 cctgtcccgc ggacagtcat cgaccttctc gagcaacatg cgcgggagac caactggact
     7381 ctgccccaga actctatccc ccgcgccgac gcggtgaaag ctgctgccca gctgagtctc
     7441 gacatgcacc agatcgacat gatcagcctc gatcactggt tccggtcgag actgtactga
     7501 tggcacacgg atcactctcg ccaaaacacc tgtctcccaa ccttttcagc cctctccatt
     7561 gttcagcctc agcgatcaat acgacctatt tcgcagtcac tacaggcaaa cagagagctc
     7621 tgatcgacga aaaccacggc tacacaaaga cggcagtgat cgcgcacacc aaagaagcgg
     7681 cgttcacgcg actggcagca gcctggggct cccttccgtc cgccttcgcc gccgaatgca
     7741 gtgtgtggcg atcatgtcgt gcgcgacggg accgtcggga tttccgacat caagggagaa
     7801 agttctccgt ctcaacgacc tcggctaccg tcatctgaac cagaaaacgg gagaacagat
     7861 gctcactgcg gatattttgc tgtcgggccc gcgcggacgc cggatgctgc tcgcgtatgc
     7921 gatcgacgcc gaacgcgccc tgcgtcccga gccaggcgac gacatgttcc gcatcaacgc
     7981 tcaccgggcg gcgtatcacc tcgacatcgg acaaggaacc tcccaagtgt tgttcggccc
     8041 gggcgccgaa gaggcccggc agaccatagt caccgcggag ggcgtggcgc gcagcctcac
     8101 ggaaatatcg ctccccgacg tcaccgccgt cgatctacgc tccgccctcg cagaagcggt
     8161 cgatgcggcg cgttactggc aggatcccga cggagaagac gtcctcgtcg ccaccgaagt
     8221 tatgcgagat gagcttcgac gcgtcgccga gcacatcgcc ggctccgaat gcgcgcggtg
     8281 gtggtcaacc tcgctcgcct catccgacca atggtcgatt ctgagcagag gtgaggaatc
     8341 tccggacaca gacgaagtgc ctgctgcgca acaactcctc agcgagtggc gcacgagcac
     8401 cctggaatcg gaagcgagag ctgaacgtga ccgcccggtg gaccccgcgg cgaactggtc
     8461 aggttattgg tggtcgaatc cacccactca gggttcgacg agggcgctgt tcgacgggac
     8521 tccggcgggc ttgtggttcg tcgaagacag catgggatgg gaacgggtcg tcgtccgagc
     8581 attgcggcca ccgactggcg cacgcatcta cgagatcgac agcgcgcaag cctggtcgga
     8641 gctgtgcaga cggttcccgc tcgaggtcac cgcagaaacg cggcaggttt ggtatcggac
     8701 taccggccga tcaggcgggt gggtcatccc cgactgggtg caggtcgcgg aaagatttga
     8761 gggagtgcac cttaccgtga cgggatacct cgcggcagca ggaaccgcga tcgaggtcga
     8821 taccgagacg gcgagcgtca tcgcgggctg ggcacccgac gaaacatact ggttcaccga
     8881 tgctgccacc tacatttccg agccgtgtgc ttgggtgctg cacgacggcc cggaagtgac
     8941 cgaccagccc gcatggacgc tggaatcgca gcaatgaccc gcacgtgagg gtctgctagg
     9001 catgaggttc cgtgaggaat cgatcgcagt tgcccttcga gtcgagtcaa ctgccccgtg
     9061 cgcggtcggc aggagcactc acgtgcacgc agacatgcgg aagggcggta ccccgcccag
     9121 gccgatccga acccgagtgc cctagatcac aaaggtaaac ctatccacaa atcgccaaat
     9181 gtcctacatt tgactcatgc cggattcccg tatcgcaccc accgcacgca acatcccact
     9241 ttcagtgcag atcgcggaac aactgcggtc ccagatcact tctggcaact gggaattagg
     9301 ggagaagatc cccggcgagc atcaacttgt cgaaatgacc ggcgcgagcc gcaatacggt
     9361 ccgggaggcc attcgcggcc tcgtccatgc cggcctcctc gaagcacgcc cgggagacgg
     9421 aacctacgtc tgcgcgagct cagaactcga aattgcactt caaagacgtg cagcggcgga
     9481 gaacgtgctc gaaatcttcg aagcccgtga agctttggag ctctacgccg cccgactcgc
     9541 agccgaacga gccctgcccg aagacatcga ccgaatgact cgggctttgg atggccgaga
     9601 cgctgagccc gacgtcgcca aatccgtcga gtacgacctc gacttccacc tggagatgtt
     9661 cgcagcgagc gggaaccaac tgatcacgga catctaccgc agtctcgatc gctctgacac
     9721 catgagcatc ccacaaggta agtccgacaa ggagactcga cagctgttca tcggaccttg
     9781 gaccgacgac gacccacacc ggcagctgct tgcggccatc ggtgaccgcg atccggacgg
     9841 cgcagccacc gcagtggtcc gactgatgga ccacagccga tccctctttc tgcaaaacat
     9901 gccgaagtag ctgacacaca agaggttcag cgcacagaca atctcagaag ggcacaccta
     9961 tggctaacgt cacctccgac accgaaaacg tggcgtctgc cccgagcgcg ataccgataa
    10021 tgcgccgagg actcgttatc gctggcattc ttctcatcgc agccaatctt cgagcctcaa
    10081 tcacagtcgt cggcccgcta ctcggtgagg tacgcaccga tctcgatatc tcgtcgacaa
    10141 cagcatcagc gctgatcagc cttccactgc tgtgcttcgc agtgttctcc cccgttgccc
    10201 cggtcatcgc agctcggtgg ggaatggaac ggacactcgc agcagcactc ggcatgctcg
    10261 ccttagcgat cgtgatccgc tctgttccgt ggatgcctgc gctgtggatc ggtaccgtat
    10321 cgctcggcat gtccatcgca acaatgaacg ttctactgcc ggcgctcctc aaacgagact
    10381 tcccgaccga agccggccgt ttgaccggca tcttctcggc ggtctctgca gccgtcgccg
    10441 caaccgcatc cggacttgcc gtgccgatcg ccggtctcac cgatcacggt tggcggatcg
    10501 cattcggaat ctgggctgct atggctctca taggatttgg ggtgttcctt ccccaacttc
    10561 gccgacgcac gcttccgaaa cacgatcatg ctgcggcttt ggattcccat ccaggcggcc
    10621 atcgctcccc ctggcgctca gcactcgggt ggcagatcac aatattcatg ggcgtccaat
    10681 ctctcttctt ctacaccgtt ctgacatggt ggcccaccat tgaagaatca cacggcacta
    10741 ccgcggctac cgccggactg catcaaggca tcatgcaaat attctcgatc gtcggcagcc
    10801 tcctcacagc agtgctcctg cacaaactgc ccaaggatca gagaggagcc gtcctcgggc
    10861 tggtgtcggt cgccgcagtg gcagttct
//
