LOCUS       NZ_CP021070            11398 bp    DNA     circular CON 04-JUN-2024
DEFINITION  Mesorhizobium sp. WSM1497 chromosome, complete genome.
ACCESSION   NZ_CP021070
VERSION     NZ_CP021070.1
KEYWORDS    RefSeq.
SOURCE      Mesorhizobium sp. WSM1497
  ORGANISM  Mesorhizobium sp. WSM1497
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 6666492)
  AUTHORS   Brewer,R., Haskett,T.L., Ramsay,J.P., O'Hara,G.W. and Terpolilli,J.
  TITLE     Complete genome sequence of Mesorhizobium sp. WSM1497
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6666492)
  AUTHORS   Brewer,R., Haskett,T.L., Ramsay,J.P., O'Hara,G.W. and Terpolilli,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-APR-2017) School of Biomedical Sciences, Curtin
            University, Kent street, Bentley, WA 6102, Australia
REFERENCE   3  (bases 1 to 6666492)
  AUTHORS   Ramsay,J.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAY-2016) School of Biomedical Sciences, Curtin
            University, Kent Street, Bentley, WA 6102, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 18-FEB-2017
            Assembly Method                   :: SPAdes v. 3.10.0
            Assembly Name                     :: WSM1497_pacbio
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 350.0x
            Sequencing Technology             :: PacBio; Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_001672455.2-RS_2024_06_04
            Annotation Date                   :: 06/04/2024 02:19:27
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,874
            CDSs (total)                      :: 6,812
            Genes (coding)                    :: 6,671
            CDSs (with protein)               :: 6,671
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 86 of 141
            Pseudo Genes (incomplete)         :: 87 of 141
            Pseudo Genes (internal stop)      :: 20 of 141
            Pseudo Genes (multiple problems)  :: 46 of 141
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 13:49:45
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 2957512
            Orig. end                         :: 2968910
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP021070.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..11398
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="6"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..11398
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..11398
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:6398](-)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..6398)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     misc_feature    complement(60..73)
                     /note="TFBS match to RpoN, Regulator of nitrogen
                     assimilation and virulence, confidence: medium, score:
                     16.73"
                     /tool="antismash"
     gene            complement(232..1353)
                     /locus_tag="A9K65_RS14250"
                     /old_locus_tag="A9K65_014260"
     CDS             complement(232..1353)
                     /GO_function="GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /GO_process="GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /NRPS_PKS="Domain: Aminotran_1_2 (39-367). E-value:
                     3.8e-41. Score: 133.3. Matches aSDomain:
                     nrpspksdomains_A9K65_RS14250_Aminotran_1_2.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_1_2"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1019: aminotransferase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018426844.1"
                     /locus_tag="A9K65_RS14250"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A9K65_014260"
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="WP_013530416.1"
                     /sec_met_domain="Aminotran_1_2 (E-value: 4.2e-39, bitscore:
                     133.3, seeds: 48, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MEQSVKPASSLPLNPHIAALPPYNAGMNISMARALSGRNDVARLA
                     SNENPDGCSPAVMAALASSSFEPWRYADPACTALRAALGEKLAIDPGMIVAGNGSEEMI
                     AAISRAVLVPGASVVTTVPSFGLHEIEPLAAGADVVKIAMTPEMGFDLAALEAAIAVGP
                     RIVFISSPWNPVGPGLDHAGLRRLIGAVRPGTLLVLDEAYFEFADPGAPDGIEVLRNSS
                     IDHVVLRTFSKAYGLAGLRVGYAVCSGFELARVIAAAKTPFNVNAAAQVAAIAALADES
                     WMKASAGRVRAERVRTAAALKGLGFRVAQSQTNFLFFDCKGDSSSVAAGLLKQGIIVKA
                     WRETGYEHYLRATIGLPADNDRLITALQGLAGR"
     aSDomain        complement(253..1236)
                     /aSDomain="Aminotran_1_2"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_A9K65_RS14250_Aminotran_1_2.1"
                     /evalue="3.80E-41"
                     /label="A9K65_RS14250_Aminotran_1_2.1"
                     /locus_tag="A9K65_RS14250"
                     /protein_end="367"
                     /protein_start="39"
                     /score="133.3"
                     /tool="antismash"
                     /translation="DVARLASNENPDGCSPAVMAALASSSFEPWRYADPACTALRAALG
                     EKLAIDPGMIVAGNGSEEMIAAISRAVLVPGASVVTTVPSFGLHEIEPLAAGADVVKIA
                     MTPEMGFDLAALEAAIAVGPRIVFISSPWNPVGPGLDHAGLRRLIGAVRPGTLLVLDEA
                     YFEFADPGAPDGIEVLRNSSIDHVVLRTFSKAYGLAGLRVGYAVCSGFELARVIAAAKT
                     PFNVNAAAQVAAIAALADESWMKASAGRVRAERVRTAAALKGLGFRVAQSQTNFLFFDC
                     KGDSSSVAAGLLKQGIIVKAWRETGYEHYLRATIGLPADNDRLITAL"
     misc_feature    1337..1352
                     /note="TFBS match to RutR, Regulator of the pyrimidine and
                     purine metabolism, confidence: medium, score: 22.72"
                     /tool="antismash"
     gene            complement(1396..2523)
                     /locus_tag="A9K65_RS14255"
                     /old_locus_tag="A9K65_014265"
     CDS             complement(1396..2523)
                     /GO_function="GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /GO_function="GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="other (smcogs) SMCOG1094: ferredoxin"
                     /gene_kind="other"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013530417.1"
                     /locus_tag="A9K65_RS14255"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A9K65_014265"
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="WP_064992562.1"
                     /transl_table=11
                     /translation="MNAFTARDTIARSGFRDLKVVAKVRESALITSFHIEPVDPQDWRD
                     FQPGQFLVFRIPVAREAAGERGYVLRNYSISCSPDSASYRISVKREAAPGPGLPDGISS
                     CFLHDRIDVGDVLQAEGPRGDFVLDKASNRPVVLLSGGVGLTPMVSMLHALASLPDRRA
                     VFIHACDNGDVHALRDEVTDLVSTRPGLTAHFCYRLPSERDKAEQRFHSEGMISRDVLQ
                     RLLPIDDYDFYLCGPPPFMQAIYVMLRELGAPRYRIAYEFFGPATVLEPDAVRVPAVPA
                     QPARIEPAGEATTVEFRKSGLTAVWDGSAVSLLDFAENQGLLPEFSCRAGICGTCKSRL
                     ISGDIAYFEEPLDELGSGEVLLCCSKPRGSVVLDI"
     gene            complement(2520..3560)
                     /locus_tag="A9K65_RS14260"
                     /old_locus_tag="A9K65_014270"
     CDS             complement(2520..3560)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013636820.1"
                     /locus_tag="A9K65_RS14260"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A9K65_014270"
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="WP_013530418.1"
                     /transl_table=11
                     /translation="MFLRNCWYVAAWDHEVADRLVPTRILGEDIVLYRRTDGVVAALED
                     ACPHRKLPLSMGRIKGDTVECGYHGLTFDSTGTCTRVPGAEKIPHVACVRSYPIAERYG
                     LLWIWMGEAAKADPALIFAVDHWGDPAWGVNRGESMALDCNYLYMTDNLLDPSHVSWVH
                     QSSFGGVQAMEDAPLETTVGADGVTVWRWVVDAAPAPFYAPFLKFSGNCDRKQHYEVRY
                     PSNAIIKAIFVPARTGGEGKPLHEDVFLMDSYNFMTPVDENQTKYYWFQMRNFAPDDLE
                     VSRQFATSVRGAFDEDRTVLNAVHKGMANKRTPNLDLKIDVGPLRFRRNLAKLIASESQ
                     QLAAAE"
     gene            3683..4600
                     /locus_tag="A9K65_RS14265"
                     /old_locus_tag="A9K65_014275"
     CDS             3683..4600
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1031: LysR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013530419.1"
                     /locus_tag="A9K65_RS14265"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A9K65_014275"
                     /product="LysR substrate-binding domain-containing protein"
                     /protein_id="WP_084015473.1"
                     /transl_table=11
                     /translation="MKKTPPLRAIQAFEAFGRRGSVTAAANELGVSVGAVSQQIRKAED
                     ALEARLLERRGRSVALTALGRVYHAAVAVGFEHIRDAQDLIEQARSADTLTISCLPSLA
                     SKWIGPQLLDWQVDHPGATVRLVGAETEPRLGAEQVDFRISYGTKYRDFEHYTELFTDW
                     VVPACSPTLLAKHPVGKPADILDLPLLSIEWARDHRSPPTWAEWAASIGVVHRRTSGEV
                     AFSLSSAAIDAAANGRGFVLAQLSMAADDIASGRLVAPFDKRIRLPEPYFLAWDRAILE
                     KPFGPELRAWIVSISKRLGATLAG"
     gene            complement(5001..6398)
                     /locus_tag="A9K65_RS14270"
                     /old_locus_tag="A9K65_014280"
     CDS             complement(5001..6398)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: YcaO"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF014661.4"
                     /locus_tag="A9K65_RS14270"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A9K65_014280"
                     /product="YcaO-like family protein"
                     /protein_id="WP_064992561.1"
                     /sec_met_domain="YcaO (E-value: 5e-45, bitscore: 153.2,
                     seeds: 127, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MLLRGPHWITLALLMHHDRPMTVNQDDPTWDRQSSEDDLDVLKNL
                     AATLVAAPENGLPLLHFATRYDIGVEVFDVPGGFLAMSNIAPFEGNEKRFTASGKGFDA
                     GTAVLSCLGEAAEISSWACRAEDVTASARIGSPGDGMRIDAIQVLGFSLEQIRHRDRLN
                     RVWRGWDSIPSTDHLNNPQLWASVRSFHDDRVALCPAFLCYGRFGDVAYGDCSLNVDTN
                     GCAAGTTRADARTRALLELVERDATGIWWHRGSLRKRLDIARLDDQGLASHIQQHRQET
                     GRRLWLLDISSFRTAIVTAAISCEDSGEHMAVGFGCSFDMVAAARSAFLELIQSEAAMQ
                     AHAERAERPRNKTMSDDDRRMTRWKRFADVRNFRFAIGKPADGLESAGESNVTDLLNEI
                     GEVCGSQVWFADLSRSEIGIPVAKAICEGLSHFKARWGCHRNAAPSIASDSSKTAGRLS
                     QARKLLI"
     gene            6564..8474
                     /locus_tag="A9K65_RS14275"
                     /old_locus_tag="A9K65_014285"
     CDS             6564..8474
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="A9K65_RS14275"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="A9K65_014285"
                     /product="hypothetical protein"
                     /protein_id="WP_064992560.1"
                     /transl_table=11
                     /translation="MGKRKACAILAYLLLSGRSRETRGRLAALLWSEQDEEHARGSLRQ
                     CIRDLRRLASETRLNYLAVDKLVLGIDPTAIRSDIDELERALDDRNYDGALLLLAKARD
                     GLCAGLDDCDPVFDNWLRMQSTMITDRLVTRLAAWLAAETDPRQRKSIAACILQLDPFF
                     DDAVVETIRRVCEESGYARALAFFDRYAAELKADMDLRPPAEILHLLEALKPGNDGEKR
                     AADPDLAQSDHDSRIPGKPTIAIIIARREAENEHLQFALATELLASISRFRHWTAVSTE
                     LQAGEFSLDQMHQAAGEHVDIGVLIRLIDTDGPAVFQITCYDFLQEGEAFVLTVPSDPS
                     SWRSAFNEVCARFSARLQIALSSARLHRISQASPNHFSAYDCWLDGQRLSIMWRKDTEE
                     AAIARYEEAIRLDPDLSCAYSSLAAVLNSRWIVVPGASSIEQDLKRAFELAKRSVLLDP
                     FDHRNQVNLAWSHLLARRWELADFHFSLAHEQNAGNPATLIAYALASSFMGDHQRASEL
                     SKRSFDLNPMPDAHYHGYQATIAFLANDLEGCVAAAVKSDQLFADIHGWSAAALALLKR
                     NREAGDEFRRFLRNLTAAWQGPGRLDRAVAVEWFKTAFPIRLPVDQEKLARGIELAAQS
                     G"
     gene            complement(8510..8911)
                     /locus_tag="A9K65_RS14280"
                     /old_locus_tag="A9K65_014290"
     CDS             complement(8510..8911)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="A9K65_RS14280"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="A9K65_014290"
                     /product="hypothetical protein"
                     /protein_id="WP_084015567.1"
                     /transl_table=11
                     /translation="MVKFKDVQFEALGEFIQDLIKDPGKQAVFRNKDQTQMKELLRGFM
                     TPRDKTWEEITIVAHFDQDLIVNIAFPFTGDVEQTIATIAPADGPGEDYTFPDHYNLDP
                     NAGPPDQIKKNRLSAYHKRLGDYVMSRCK"
     gene            complement(8959..10632)
                     /locus_tag="A9K65_RS14285"
                     /old_locus_tag="A9K65_014295"
     CDS             complement(8959..10632)
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF015083.4"
                     /locus_tag="A9K65_RS14285"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A9K65_014295"
                     /product="peroxidase family protein"
                     /protein_id="WP_064992558.1"
                     /transl_table=11
                     /translation="MLHGETSSFEAANIPNRSNQNSDDGLNDKPTLRGIRLFPSLADVP
                     IALFDATEDESAIDRTKAGLLDITAEMVGPTDGLEGSDLSPTPAGFTYFGQFVFHDIVF
                     SQIFGLPKPTGKTHHLKNASSQGLDLSGLYGRGPIVDAHLYDVADGADLSACRFPIGLP
                     CMKGSMLPVDGKTMQGRDLPRIDFSGKFISVEGRRSPYRPLVADPRNDDNLVLSQLLCT
                     LMGIHNRIVDLLLGIGTDPAEAYKRARIYLTSVYRTVIINDYLRKVLAPEIWGHFFASD
                     DFLGAGVSALETFNALPLEFTFGASRFAHAMVRQFYVVNDRSVEEPANLRKMLSFSSLR
                     PGGEIPIPENWIVDWNRFASADTPSKAQSARRIGPFLSKELTIAGLSAELNGTVRPVAF
                     MDCWRCYDLGLPSGQDVAGAVKTALGDNSIDVPVLTGNDMLPTAACAKRYVYNAGRLRA
                     ALQRYPEFLRQTPLSYYIIQEASVLGNDGSHLGPVGSYVVAATIASALFKAADTDITSR
                     RLIPDVEPKTLAGLLDLNDTAKVSDEVLSGLINSQDFQQR"
     misc_feature    complement(11082..11095)
                     /note="TFBS match to RpoN, Regulator of nitrogen
                     assimilation and virulence, confidence: medium, score:
                     16.82"
                     /tool="antismash"
ORIGIN
        1 gaatctcatt ttgactgcag atgcgcacca ccgcggcgac ctcgccggtc gatgaaggcg
       61 caacgacgat gccggcggcg caatccgtcc cgttcgaacc aaggtcgatc gccccggcat
      121 catcactggt ccgcacattc gaggcgccaa cgacacggcg gatcgcgtcg acaaccgaca
      181 atggcaccgc ttgctcgatc cagttcgttt gcctgggaga cgcgtggccg ctcatcgccc
      241 cgccaatccc tgaagcgccg tgatcagccg gtcgttgtcg gccggcaagc cgatggtggc
      301 gcgcagatag tgctcgtagc cggtttcgcg ccacgccttg acgatgatcc cctgcttcag
      361 gagaccggcg gcaacgctgg agctgtcgcc cttgcagtcg aagaacagga agtttgtctg
      421 cgactgggca acccggaaac caaggccctt aagtgcggcg gcggtgcgca cacgctcggc
      481 tctgacgcgg cccgccgacg ccttcatcca gctttcgtcg gccagtgccg caatagcggc
      541 gacctgtgct gccgcattga cgttgaacgg cgtcttggcg gccgcgatca ctcttgccag
      601 ttcgaagccc gaacaaacgg catagccaac gcgcagcccc gccaggccat aagccttcga
      661 aaatgtgcgc agcacgacat ggtcgatgct gctgttgcgc aggacctcga tgccgtcggg
      721 cgcgccggga tcggcgaatt cgaaataggc ctcgtccagg acaagaagcg tgccgggccg
      781 cacggcgccg atcagccgtc gcaggccagc gtggtcgaga ccgggcccga ccggattcca
      841 cggcgaggat atgaaaacga tccgtggccc gacggcgatc gccgcttcga gcgcggcaag
      901 atcgaacccc atctcgggtg tcatcgcaat cttgacgaca tcggcaccgg cagccagcgg
      961 ctctatctca tgcagcccga agctcggcac cgtggtgacg accgaggcgc ccggcaccag
     1021 gacggcgcgc gagatggcgg cgatcatctc ctcggaacca ttgccggcaa cgatcatgcc
     1081 aggatcgatc gccagttttt cgccaagcgc cgcccgcagc gccgtgcagg ccggatcggc
     1141 atagcgccat ggctcgaagg atgaggaggc caatgcggcc atgacggcgg gagaacaccc
     1201 gtccgggttc tcgttgctgg ccagccgggc gacatcgttg cggccactca gcgcccgcgc
     1261 catcgagatg ttcatgccgg cattgtaggg cggcaatgcg gcgatatgcg gattgagcgg
     1321 caggctcgac gctggtttga ccgactgttc caaatcccga cttccgtttc cccgcgccca
     1381 cgcgcggcaa tcttgtcaaa tatcgaggac gactgatccg cgcggcttgg agcagcacaa
     1441 cagcacttcg ccgcttccaa gctcgtccag cggttcttcg aaataggcga tgtcgcccga
     1501 gatcagccgc gacttgcatg tgccgcagat gccggcccgg cagctgaatt ccggcaacag
     1561 accttggttc tcggcgaagt cgagcaacga tacggccgag ccatcccaga cggccgtcag
     1621 gcccgacttc cgaaattcca cggttgtcgc ttccccggcc ggctcgatcc ttgccggctg
     1681 cgctggaacc gcgggaactc gcactgcatc gggctcaagc accgtcgccg ggccgaagaa
     1741 ttcgtaggca atgcgatacc ttggcgcgcc aagctcgcgc agcatgacgt agatcgcctg
     1801 catgaacggc ggcgggccac agaggtagaa atcatagtca tcgatcggca gcaaacgctg
     1861 cagcacgtcc cgcgaaatca tgccttcgct atgaaagcgc tgctcggcct tgtcgcgctc
     1921 ggacgggagc cggtagcaga agtgtgcggt caggccggga cgggtcgaga ccaggtcggt
     1981 cacctcatcg cgcaaggcat gcacatcgcc gttgtcgcag gcgtgaatga agacggcacg
     2041 gcggtccggc aacgaagcca acgcatgcag catcgacacc atcggcgtca gcccgacgcc
     2101 gccgctgagc agcacgactg gacggttgct ggccttgtcg agcacgaaat cgccgcgcgg
     2161 cccttccgcc tgaagcacat caccgacgtc gatacggtca tgcaggaagc acgagcttat
     2221 cccatcggga agccccggcc cgggtgctgc ctcacgcttc acgctgatcc gataggaagc
     2281 gctgtctggg gaacaggaga tactgtagtt gcgcagcaca tatccccttt cgccggcggc
     2341 ctcccgcgct accggaatcc ggaacaccag gaactggccc ggctggaagt cgcgccagtc
     2401 ctgtggatcg accggctcga tatggaatga agtgatgagg gcgctttcgc gcaccttcgc
     2461 gaccactttc aggtcgcgaa atccgcttct tgcgatggtg tcgcgagcgg tgaatgcatt
     2521 cattcggcag ctgccaattg ctggctctcg ctggcgatca gcttggccag gttccggcga
     2581 aagcgcagcg gtccgacatc tatcttcaga tcgagattgg gcgtgcgctt gtttgccatg
     2641 cccttgtgca cggcattgag cacggtgcgg tcctcgtcga aggcgccgcg cacagaggtg
     2701 gcgaactggc gggaaacctc caaatcgtcg ggggcgaaat tgcgcatctg gaaccagtaa
     2761 tatttcgtct ggttctcatc gaccggcgtc atgaagttgt aggagtccat gaggaacacg
     2821 tcctcgtgca gcggcttgcc ctcgccgccc gtgcgggccg gcacgaagat cgccttgatg
     2881 atggcgttgg aggggtagcg cacttcatag tgctgcttgc ggtcgcaatt gcctgagaat
     2941 ttgaggaaag gcgcatagaa gggcgccggc gcggcatcga ccacccagcg ccacacggtc
     3001 acgccgtccg cgcctacggt ggtttcgagt ggcgcatcct ccatcgcctg aacgccgccg
     3061 aaggaggact ggtggaccca agacacatgc gaggggtcga gcaggttgtc ggtcatgtag
     3121 aggtagttgc aatcgagcgc catggactcg ccgcgattga cgccccatgc cggatcgccc
     3181 cagtggtcga ccgcgaaaat cagcgcgggg tcggcctttg ccgcttcgcc catccatatc
     3241 cacagcagcc cgtaacgttc ggcgatcgga taggaacgaa cgcatgccac atgcggtatc
     3301 ttttcagcac cgggcacgcg cgtacaggtt ccggtgctgt cgaaggtcag cccgtgatag
     3361 ccgcattcga cagtgtcgcc cttgatgcgg cccatggaca gcggcagctt gcggtgggga
     3421 caggcatcct cgagcgccgc caccacgcca tcggtccggc ggtagaggac aatatcctcg
     3481 ccaagtatcc gggtcggcac cagcctgtcg gcgacctcat ggtcccatgc ggcaacgtac
     3541 cagcagtttc gaagaaacat ggcgtcctcc cgagcttgtg acgcctaaac gaatatcttg
     3601 cgggcctggg acgacatagg gcttgcatct gcgttctatt ttagaaaaac taaatcaagc
     3661 tgcccccagg atcgttgccc aggtgaagaa gacgccccca ctgcgtgcga tacaggcctt
     3721 cgaggctttc gggaggcgtg gcagtgtgac ggcggccgcc aacgaactcg gcgtctccgt
     3781 cggcgcggtc agccagcaga tccgcaaggc cgaagacgcg ctggaggcgc ggcttttgga
     3841 acggcgcggc aggagtgtcg ccctcaccgc gctgggccgc gtttaccacg cagcggttgc
     3901 cgtcggcttc gaacacattc gcgacgccca ggatctcatc gaacaggccc ggtccgcgga
     3961 cactttgacc atctcctgcc tgccgtcgtt ggccagcaaa tggatcggcc cgcagctcct
     4021 ggattggcag gtcgaccacc ccggcgcgac cgtgcggctg gtgggcgccg agacggagcc
     4081 aaggctgggc gcggaacaag ttgacttccg tatttcgtac ggcacgaaat atcgtgattt
     4141 cgagcactac accgaactgt tcaccgactg ggtggtgccg gcctgctcgc cgacgctgct
     4201 ggcgaaacat ccggtcggca agcccgccga tattctcgat ctgccgcttc tcagcatcga
     4261 atgggcccgc gatcaccgct cgccgccgac atgggcggaa tgggccgcaa gcatcggcgt
     4321 ggtgcaccgc agaacatccg gcgaagtggc cttttccctg tcgagcgcgg cgatcgatgc
     4381 ggcggccaac ggaaggggct ttgtgctggc gcaactgtcg atggctgccg acgatatcgc
     4441 gtcgggcagg ctcgtcgccc cgttcgacaa gcggatcagg ctgccggaac cttatttttt
     4501 ggcttgggat cgggccattc ttgagaagcc ttttggtcct gagctgcgag cctggattgt
     4561 ttcgatttcc aagcgactgg gcgcaacctt agccggatag tcgaacaccg atctggagac
     4621 acaagtttgc atctgccgag ttcgcgcgca ccagcgtctt aagagatcca gacattggtc
     4681 ggcggtgtgc ggtcccacgg attgcttcgg tttgccctag atatctcgac aaaagtccgc
     4741 ttttttcgcc cttcttgcac cgctagccgt cgggcggctt tgcgacccaa aacagcagcg
     4801 ttcgcgccct caagtcaaca ggggttcact caacttccct gctgtacgag atcccggcga
     4861 actcggagcc cctgcttgag caaatcccga cgtacacgaa gtcctggccc cgcccacaat
     4921 cgtaacgccg gcttcctcat cagcgtcctg taaccggcga cgggtctcca acgtgcccgt
     4981 gtcgtcattg cctgtaacaa ttagatcaaa agttttctgg cctgcgagag gcggcctgct
     5041 gttttgctcg aatcacttgc gatggaaggt gcggcgtttc ggtggcagcc ccagcgcgct
     5101 ttgaaatggg ataatccctc gcagatcgcc tttgcaacgg ggataccaat ctcggatcgc
     5161 gaaagatcgg cgaaccagac ctgcgaacca caaacttcgc cgatttcatt cagcaggtcg
     5221 gtgacgttgc tttcgccagc gctttccagg ccgtcggcag gcttgccgat ggcgaaacga
     5281 aagttgcgca cgtcagcgaa acgcttccag cgggtcatcc gacgatcgtc atcgctcatc
     5341 gttttattac ggggccgttc tgccctttcg gcatgggcct gcatcgcagc ttcggattgt
     5401 atcaattcca aaaacgcgga ccgggcggca gcaaccatat cgaagctgca cccgaaaccc
     5461 accgccatat gctcgccact gtcttcgcag ctgatcgccg ccgtcacgat ggccgtgcgg
     5521 aaggacgaga tatcgagaag ccagagcctg cgtcccgttt cttggcgatg ctgctggatg
     5581 tgagacgcca agccttggtc atcaagccgc gcgatatcca gccgctttct cagggagcct
     5641 cggtgccacc atatccctgt ggcgtctcgc tcgacgagtt caagcaaggc gcgggttctt
     5701 gcatcggccc gcgttgtgcc agcggcgcaa ccattggtgt cgacgttcaa gctgcaatcg
     5761 ccatatgcga cgtcaccaaa gcggccatag cagagaaacg ccggacaaag cgcgacccta
     5821 tcgtcatgga agctgcgaac ggacgcccat agctggggat tgttcagatg atccgttgaa
     5881 gggatactgt cccagccacg ccacactctg ttgagccgat cgcgatgtcg gatctgttcc
     5941 agcgaaaagc ccaatacctg aatcgcatcg atcctcattc catcgcctgg agagccgatg
     6001 cgtgctgacg ccgtgacgtc ttcggctctg caggcccagc tggaaatctc cgcggcctct
     6061 ccgaggcaag acagcacggc cgtgccggca tcaaagcctt tgccacttgc cgtgaatcgt
     6121 ttctcattcc cttcgaatgg agcaatgttg gacatagcca ggaaaccacc cggaacatcg
     6181 aacacttcaa cgccaatgtc gtagcgtgtc gcgaagtgca aaagaggcag cccattctcg
     6241 ggcgcagcga caagggtcgc agccagattc ttcaaaacat ccaaatcatc ttcactcgac
     6301 tggcggtccc aggtcgggtc gtcttggttc accgtcatcg gccggtcgtg atgcatcagc
     6361 agagcaagcg ttatccaatg cggcccgcgc aacagcatgt tggctctttc aatatcttgg
     6421 cttctgctgt ttacgtaggg taagcggtgg gcgcccgttg gcgaccgaga cgatagccgg
     6481 cggagttgac aatttccaca gacggtctgg cgattaggct aatcggcggt ttcgagcttc
     6541 gcaagggctc ggagacgttc tcgttgggca agcggaaggc ctgcgcgatt ctcgcctatc
     6601 tgttactgag cggcaggtca cgcgagacgc gcgggcgact ggcggcattg ctttggagcg
     6661 agcaggacga ggagcatgcg cgcggatcac tgcgccagtg tataagggat ctccgacgcc
     6721 tggcaagcga gaccaggctt aattacctcg cggtcgacaa gctcgtcctg ggaatcgatc
     6781 cgactgcaat ccgttccgac atcgatgagc ttgaacgtgc gctcgacgac cgcaattatg
     6841 acggtgcgct gctcctgctg gccaaagcca gggatggact ttgcgccggg ttggacgatt
     6901 gcgatcctgt cttcgacaac tggctgagaa tgcagtcgac gatgataacc gacaggctgg
     6961 tgacacggct cgcagcctgg ctggcggccg agacagaccc gagacagcgc aaatccatcg
     7021 ctgcgtgcat cctgcaactt gatccgtttt tcgacgatgc cgtggtggag accattcgga
     7081 gggtctgtga ggaaagcggt tatgcccgcg ccctagcgtt cttcgaccgc tacgcggccg
     7141 aactcaaagc ggacatggat ctgcgaccac cggcagaaat cctccacctt ctagaggcgc
     7201 tcaagccggg caacgacggt gaaaagcggg cggctgaccc cgaccttgcg caaagcgatc
     7261 acgacagccg catcccaggg aagccgacaa tcgccatcat cattgccagg cgcgaagcag
     7321 agaacgaaca cctgcaattt gccttggcga cagagcttct ggcttcgatt tcccgattta
     7381 ggcattggac agccgtgtca acggagttgc aagccggtga attctcactg gatcagatgc
     7441 atcaggcggc aggcgaacac gtggatatcg gcgtcctcat ccgtctgatc gacacggatg
     7501 gaccagcagt gttccaaatt acctgctatg acttcctgca agaaggagaa gccttcgttc
     7561 tcaccgttcc gtcggatccg tcttcctggc gctcggcctt caatgaagtt tgcgcacgct
     7621 tttccgcgcg cctgcagatt gccttgtcca gtgccaggct gcatcgcata tcgcaagctt
     7681 ccccgaacca tttctcggcc tatgattgct ggctggacgg acagcggctt tcgatcatgt
     7741 ggcgaaagga caccgaagaa gctgccatcg cccgctacga ggaagccatc cggcttgatc
     7801 ccgatctgtc ctgcgcctat tccagcctcg ctgcggtgct caactcgcgt tggatcgtcg
     7861 ttccgggtgc atcgtcgatc gagcaggatc taaagcgcgc attcgaactg gccaagcgtt
     7921 cggtcctgct cgacccattc gaccaccgca accaggtaaa tctggcctgg agccaccttc
     7981 tggcgcgacg ctgggagctg gccgattttc acttcagcct tgcacatgag caaaatgccg
     8041 ggaaccccgc gacgttgatt gcctacgcgc tggccagctc cttcatgggt gatcatcaac
     8101 gggcatcgga gctttcgaag cgcagtttcg acctcaatcc catgcccgac gctcactatc
     8161 atggctacca ggcgaccatt gcctttctcg ccaatgatct ggaggggtgc gtggccgctg
     8221 cggtcaagag cgatcaactg ttcgccgaca tccacggctg gtcggcggcg gcacttgcgt
     8281 tgctgaaacg caaccgcgag gccggggatg agttcagacg ttttctacgc aacctaacgg
     8341 cggcgtggca agggccgggt aggcttgacc gcgctgtcgc cgtggagtgg ttcaagaccg
     8401 cttttccgat ccggttacca gtcgatcagg agaagctggc ccgtggcatc gaattggcag
     8461 cccagtcggg ctgacacggc cgaaccgttg ctcgcaattg cgccgggttt cacttgcagc
     8521 gtgacatcac gtagtcgccg aggcgcttat gatatgcgct caaacggttt ttctttattt
     8581 gatcgggcgg gccggcgttc gggtcgagat tgtagtgatc gggaaatgta tagtcctcac
     8641 cgggtccatc cgccggtgca atggtggcga tcgtttgctc gacgtcgccg gtgaaaggaa
     8701 aggcgatgtt cacgatcagg tcttggtcga aatgcgcgac gatggtgatc tcctcccaag
     8761 tcttgtccct cggggtcatg aacccacgca gcagctcttt catctgcgtc tgatctttgt
     8821 tcctgaagac cgcctgtttg cccgggtctt tgatcagatc ctggatgaat tcgccaagcg
     8881 cctcaaactg cacgtccttg aattttacca tgttcatctc cccctgtagc caaattgaat
     8941 gctgtagcgc tgccgctatc aacgctgttg aaagtcctgg ctgttgatca ggccgctaag
     9001 cacttcatcc gataccttcg cagtatcatt gagatcgagc agtcctgcca gcgtttttgg
     9061 ctctacgtcc ggaatgagcc tgcgagaggt gatatccgta tcggcagcct tgaacagtgc
     9121 ggatgcgatc gtggcggcca ccacatagga gccaactggg ccaagatggc tgccgtcatt
     9181 accaagaacg gacgcctcct ggatgatata gtaggagaga ggtgtttgcc tcaggaactc
     9241 cgggtaccgt tgcaaagcgg cccgaagtcg gccggcgttg tagacgtaac gtttggcgca
     9301 ggcggccgtg ggaagcatgt cattgcccgt caggaccggc acatcgatac tgttgtcgcc
     9361 cagggcggtc ttcaccgcac cggccacgtc ctgcccggat ggcaaaccaa ggtcgtaaca
     9421 gcgccagcaa tccatgaagg caaccggccg gactgtcccg ttcaattccg cactcaggcc
     9481 ggcaatcgtc agttctttcg acaggaatgg tcctatgcgc cgggcacttt gagctttcga
     9541 cggggtgtcg gctgaggcaa accggttcca gtcgacaatc cagttctcgg gtattggaat
     9601 ctccccaccc gggcgcaggc tggaaaagga cagcattttg cgcagattgg ctggttcttc
     9661 gaccgacctg tcgttcacga catagaactg ccggaccatg gcgtgggcaa agcgcgacgc
     9721 accgaaggtg aattccaaag gcagcgcatt gaaagtttca agtgccgaga ctccggcgcc
     9781 aagaaaatcg tcactggcga aaaaatggcc ccatatttcc ggagccagaa cttttctcag
     9841 atagtcgttg atgatcacag tgcggtaaac cgaggtcaga tagatcctgg cacgcttgta
     9901 agcttctgct gggtcggtgc cgatgccaag cagtagatcc acgatcctgt tgtggatgcc
     9961 catgagtgtg cagagcagtt gcgacaggac aagattgtcg tcgtttcgcg gatccgccac
    10021 caagggccga taaggcgatc gccgaccctc gacactgata aacttgcccg agaagtcgat
    10081 gcgcggcaaa tcacggcctt gcatggtctt accgtcgacc ggaagcatac tgcctttcat
    10141 gcaaggcaag ccgatcggaa agcggcaagc cgacaggtcc gccccatccg caacatcgta
    10201 gagatgggcg tcgacgattg gcccccgccc atagagcccc gaaaggtcaa ggccttgcga
    10261 ggacgcgttc ttcaaatgat gggttttgcc tgtcggcttg ggcaatccga atatttggct
    10321 gaagacgatg tcatggaaca cgaattgacc gaaataggta aagccggctg gtgtagggct
    10381 gagatcactg ccttcaagtc catcggtcgg gccgaccatt tccgccgtga tgtccagcaa
    10441 gcccgccttg gtgcggtcga tggcgctctc gtcttcggtt gcgtcaaaca aggcgatcgg
    10501 cacgtccgcc agggacggga agagcctgat gcctcgcaag gtcggcttgt cgttgaggcc
    10561 gtcgtctgaa ttctggtttg accggttcgg tatgttggcg gcttcgaagg aggacgtttc
    10621 gccgtggagc atcgaagtag acctcgtttt ccagaaggat gaatgcggcc aggcgagtag
    10681 cttggcatga atccgggagt agaaacagac ttgcccgtcg gtggtctgaa tggcgcggcc
    10741 ccagagcaga gcgacattgc cgaggcgatc atgacactga cggatcgagc ttcgggcccg
    10801 ccgagacggc ggtgaacagc acacaggaca ggatgacgcc cgcacccaga agcccgtcgg
    10861 gcgaaagccg ttcaccaagc agagcggcag cggaaatggc ggcgaacaca ccctctccgc
    10921 tgcaaatcac cgccgcatgg gacgaagtgg tgtagcgctg tgcagaagtc tgcaggcaaa
    10981 agccgacagc ggtcgagaac acaccaagca tgatgagttc tggccatgct gccataattg
    11041 cctgaaatgt cattgtgccc tgcagggcga aaggtagcgt ggcaacggcc gtgccgatga
    11101 actgtgcggc cgccgtcgcg aagggatggc catgtgcctg cacatgccgc ccgagttcga
    11161 ccgtccatac tgcaaaacag attgcagtca ggacggcgaa agcgtcgccg gcggttgccg
    11221 aaccattgaa gccgcccgcc aggagagccg atccgacgac cgtcagaatt gcgacgaata
    11281 ggatcagtgc acttggacgc tccctcatga tcagccacgc ggcgatcggc gtgataacag
    11341 tggaagcgct gataaggagg ctcgagttcg tcaccgtcgt gctgacatag gacaattg
//
