LOCUS       NZ_CP021070            22781 bp    DNA     circular CON 04-JUN-2024
DEFINITION  Mesorhizobium sp. WSM1497 chromosome, complete genome.
ACCESSION   NZ_CP021070
VERSION     NZ_CP021070.1
KEYWORDS    RefSeq.
SOURCE      Mesorhizobium sp. WSM1497
  ORGANISM  Mesorhizobium sp. WSM1497
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Phyllobacteriaceae; Mesorhizobium.
REFERENCE   1  (bases 1 to 6666492)
  AUTHORS   Brewer,R., Haskett,T.L., Ramsay,J.P., O'Hara,G.W. and Terpolilli,J.
  TITLE     Complete genome sequence of Mesorhizobium sp. WSM1497
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6666492)
  AUTHORS   Brewer,R., Haskett,T.L., Ramsay,J.P., O'Hara,G.W. and Terpolilli,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-APR-2017) School of Biomedical Sciences, Curtin
            University, Kent street, Bentley, WA 6102, Australia
REFERENCE   3  (bases 1 to 6666492)
  AUTHORS   Ramsay,J.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAY-2016) School of Biomedical Sciences, Curtin
            University, Kent Street, Bentley, WA 6102, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 18-FEB-2017
            Assembly Method                   :: SPAdes v. 3.10.0
            Assembly Name                     :: WSM1497_pacbio
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 350.0x
            Sequencing Technology             :: PacBio; Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_001672455.2-RS_2024_06_04
            Annotation Date                   :: 06/04/2024 02:19:27
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,874
            CDSs (total)                      :: 6,812
            Genes (coding)                    :: 6,671
            CDSs (with protein)               :: 6,671
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 52
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 86 of 141
            Pseudo Genes (incomplete)         :: 87 of 141
            Pseudo Genes (internal stop)      :: 20 of 141
            Pseudo Genes (multiple problems)  :: 46 of 141
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 13:49:45
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 247960
            Orig. end                         :: 270741
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP021070.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..22781
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="lassopeptide"
                     /region_number="2"
                     /rules="(PF13471 and Asn_synthase or micJ25 or mcjC)"
                     /tool="antismash"
     cand_cluster    1..22781
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(PF13471 and Asn_synthase or micJ25 or
                     mcjC)"
                     /kind="single"
                     /product="lassopeptide"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..22781
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[10000:12781](+)"
                     /cutoff="10000"
                     /detection_rule="(PF13471 and Asn_synthase or micJ25 or
                     mcjC)"
                     /neighbourhood="10000"
                     /product="lassopeptide"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      10001..12781
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="10000"
                     /detection_rule="(PF13471 and Asn_synthase or micJ25 or
                     mcjC)"
                     /neighbourhood="10000"
                     /product="lassopeptide"
                     /protocluster_number="1"
     misc_feature    complement(412..425)
                     /note="TFBS match to RpoN, Regulator of nitrogen
                     assimilation and virulence, confidence: strong, score:
                     17.22"
                     /tool="antismash"
     gene            complement(713..1792)
                     /locus_tag="A9K65_RS01170"
                     /old_locus_tag="A9K65_001170"
     CDS             complement(713..1792)
                     /EC_number="2.4.-.-"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Glycos_transf_1"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1045: glycosyl transferase group 1"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015314217.1"
                     /locus_tag="A9K65_RS01170"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A9K65_001170"
                     /product="glycosyltransferase"
                     /protein_id="WP_064992952.1"
                     /sec_met_domain="Glycos_transf_1 (E-value: 2.8e-13,
                     bitscore: 48.4, seeds: 63, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKVLFAGGNGYPPEFSGGVQSSTHHLAEQLIEHGHEAAVLAALFG
                     DGVFGFKARAKMKLLRQPAVVDSYPGYPVVRAWFPWEAAGYAVDRLKPDVAVVQCHKSV
                     PIGKALQAEGVPLVVYLRNVEFHELGGDLRELHSALYIANSDFTARTYKKEFGIEAAVI
                     PPTINPAHYSTPTTGQFVTLINPYEEKGFELAVRIAGACREIPFLFVESWKLDDDHRAR
                     IEQTIAPLGNVVLESRTSDMKTVYGRTKILLAPSKWEEAWGRVASEAHCSGIPVIGSRR
                     GGLPEAVGSGGVVLDYDAPLADWVAAVRRLWSDNQEYDRLSSLARSFAGRPELDPDRQF
                     ITFFSILDRAMQQRARQAA"
     gene            complement(1867..2316)
                     /locus_tag="A9K65_RS01175"
                     /old_locus_tag="A9K65_001175"
     CDS             complement(1867..2316)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013891481.1"
                     /locus_tag="A9K65_RS01175"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A9K65_001175"
                     /product="hypothetical protein"
                     /protein_id="WP_013528107.1"
                     /transl_table=11
                     /translation="MIEISRRSALAVLAGGVVSTGVAYATVSSFSDEDLVRVTLEKYLG
                     KLNIRIEHLQQFVLAFRDRNPWNFPSEKLSDAVTLVEELKLGAARRLLPRRKREDLLHF
                     DRWVVAEFHMLTDYAWRSSPNDPIRFTGWQSCTNPFANLEPPKLA"
     gene            complement(2330..3772)
                     /locus_tag="A9K65_RS01180"
                     /old_locus_tag="A9K65_001180"
     CDS             complement(2330..3772)
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008872476.1"
                     /locus_tag="A9K65_RS01180"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A9K65_001180"
                     /product="GMC family oxidoreductase"
                     /protein_id="WP_013528108.1"
                     /transl_table=11
                     /translation="MVLDVKPEQITKNHFDLVAIGSGFGSAFFLHEFARRRKARILVLE
                     WGRHNTHEWQLEQNVNTDIEDETTYKTNSDKPWNYTIGLGGGTNCWFAQTPRFHPNDFR
                     LKSTYGIGNDWPISYDDVEPFYCDAEDIMSISGDPDMAAMLPRSKPFPQPPHRMSTPDR
                     MMKAAQPDQHFVMPTARARVPTAQRSSCCANLRCWLCPVDAKFTVNNGLMHVFEHPDIS
                     VCLGAEVRRLDHVGGAVRSVTFSHDGKEYQVSGDLFILGANAIQSAAIMLRSGLGGEFV
                     GSGLHESYGWNFEVYLDGVDNFDGSTITTGLNFGLYDGPHRSEHAAALVYFENRWQHGM
                     RAEKGRLRQALPLVVVTENLLDPENYVTLDADDNAFVSFKAPSDYAVKGMARALEKLPD
                     LLAPLPVEKLFDRGIRPTESHVQGTLRMGTGPADSVVDSGMIHHQLRNLVVVGTSTYPS
                     CSCANPSLTAAALSLRAASRIA"
     gene            complement(3865..5196)
                     /locus_tag="A9K65_RS01185"
                     /old_locus_tag="A9K65_001185"
     CDS             complement(3865..5196)
                     /EC_number="1.1.1.-"
                     /GO_function="GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1152: nucleotide sugar dehydrogenase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008872478.1"
                     /locus_tag="A9K65_RS01185"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A9K65_001185"
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="WP_013528109.1"
                     /transl_table=11
                     /translation="MNLTVFGIGYVGLVQAAVLAEVGHQVVCVDIDAAKVERLNQGLVP
                     IFEPGLENLVKENHAAGRIKFTTDAAAAVRHGEIQMIAVGTPPGEDGSADLKYVLAVAE
                     TIGREMDKPKIVVGKSTVPVGTCEKVKARIADTLKKRGREDLAFDVASNPEFLKEGSAV
                     ADCMKPDRIIVGTDSEDTEAVMRELYAPFNRNHEKMIVMDVRSAEFTKYAANCMLATKI
                     SFMNEMANLAEQLGADIEEVRKGIGSDPRIGYHFIYPGLGYGGSCFPKDVRALIKTAEG
                     VKFDAKLLRAVEERNNAQKSVLFDKVDRYFKGNLKGRTFALWGLAFKPNTDDMREAPAR
                     VLMEALWKAGANVQAYDPEAMQECQAIYGLRDDLLLCGTKEAALRGADALLIATEWKSF
                     RAPSFDAVKDALTTPVIFDGRNLYDPKVIARHGIEYHSIGRMAA"
     gene            complement(5264..6118)
                     /locus_tag="A9K65_RS01190"
                     /old_locus_tag="A9K65_001190"
     CDS             complement(5264..6118)
                     /GO_function="GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /GO_process="GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013528110.1"
                     /locus_tag="A9K65_RS01190"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A9K65_001190"
                     /product="family 16 glycosylhydrolase"
                     /protein_id="WP_024501832.1"
                     /transl_table=11
                     /translation="MNWNPKPTTAIDAPPLAYRTGQAARRLGSLGALLLAALGGFLATP
                     SHAQDIPTSPSFVDDFKSFDRTRWYVSDGWNNGSHQNCTWSKNMVELSNGVLSLGFEKQ
                     TLKDRDYVCGEIQTKQRFGYGTYEARLKTDTGSGLNAAFFTYIGPSDKQPWDEIDFEVL
                     TKDTSKVQVNAYIAGKGKNEKLVDVPGGTEKAFNDYAFVWQKDSLRWYVNGQLVNTITD
                     PAKLPSHAQKIFFSFWGSDTMKGWMGAFADPGRKLSLQVERVAFTALGEPCQFPESLAC
                     SVK"
     misc_feature    complement(6121..6134)
                     /note="TFBS match to PsrA, Oleic acid responsive regulator,
                     confidence: weak, score: 19.17"
                     /tool="antismash"
     gene            complement(6273..6668)
                     /locus_tag="A9K65_RS01195"
                     /old_locus_tag="A9K65_001195"
     CDS             complement(6273..6668)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013891485.1"
                     /locus_tag="A9K65_RS01195"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A9K65_001195"
                     /product="hypothetical protein"
                     /protein_id="WP_024501833.1"
                     /transl_table=11
                     /translation="MASIFGFRSRDPARDRQADVSRLDRLAKLFEQIAAEIEAERTGLE
                     NRYRTTSTNAAFLVEAMENGSASDKRSSEVSALTQSILNCERRIAALSRQDGMMKELRH
                     SLDTVFDEDASSAAAAEFAWPAGAGRG"
     misc_feature    complement(6949..6964)
                     /note="TFBS match to ClgR, Caseinolytic protease gene
                     regulator, confidence: weak, score: 21.86"
                     /tool="antismash"
     gene            complement(6958..7980)
                     /locus_tag="A9K65_RS01200"
                     /old_locus_tag="A9K65_001200"
     CDS             complement(6958..7980)
                     /EC_number="2.4.-.-"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Glycos_transf_2"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013528112.1"
                     /locus_tag="A9K65_RS01200"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A9K65_001200"
                     /product="glycosyltransferase"
                     /protein_id="WP_013528112.1"
                     /sec_met_domain="Glycos_transf_2 (E-value: 8.4e-22,
                     bitscore: 76.4, seeds: 256, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSMTKSEVCVIIAAKNAAATIAVAIASALREPEVAEVVVVDDAST
                     DDTAGVARSADDGSGRLSVMRLDVNRGPSFARNAAIAGSKSPFISILDADDFFLEGRFR
                     NLFAGADWDFAADNIMLIRDDATGDATKIVAPQFAADPEFLDFERFVEGNISRRRVQRG
                     ELGFLKPVISRAFLDRHGLSYDESLRLGEDYELYARAVACGARFKVIRSCGYGAIVRAD
                     SLSGRHKTQDLKRLADADLALLAIDSLPEGSKAVLRRHERHVRDKYRLRNFLDVKAERG
                     LGSAAAYAFASLSNLVPIVQGVASDKLEALFRRVGLVSSQKPVPPLRFLMAGTSTGKER
                     "
     gene            complement(8072..9196)
                     /locus_tag="A9K65_RS01205"
                     /old_locus_tag="A9K65_001205"
     CDS             complement(8072..9196)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) NTP_transf_5"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013891487.1"
                     /locus_tag="A9K65_RS01205"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A9K65_001205"
                     /product="nucleotidyltransferase family protein"
                     /protein_id="WP_013528113.1"
                     /sec_met_domain="NTP_transf_5 (E-value: 2.2e-32, bitscore:
                     111.1, seeds: 77, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MALPDGSYERLRAAGCAGEVAYVQACLRLFFAGPGAGDVSMRHLD
                     GEKIAEIARLNKVAVFVLKALSRAPALQRPTKLFQWLDTYRRKTVSMNASCIMDSMAIQ
                     DVLRASEIDFVFLKGPFQQQLLYDDHFMKPSGDVDILVSPLGFFRARDALRKIGYEVSG
                     KSSSVWWVRFLGEQHMTRDDGPRSSTVDLHYRLQQPGSPSPRDADGFLRRKRQVEIAGA
                     TVPFTSVADTLLLSCISVAKALFNREPCAGYVCDIGASAGRLDEADQQRLLDAATAQGL
                     DDTLLLGLRAADVLLGGAGTLLSQRATRILPRIGNEDLLSMVIAPWLPSLRWPQRRTVL
                     WELCGRAPVRYLAEAGWAASADISRRIFERPASR"
     misc_feature    9409..9423
                     /note="TFBS match to SypG, Regulator of biofilm formation
                     and host colonization, confidence: weak, score: 20.0"
                     /tool="antismash"
     misc_feature    9415..9430
                     /note="TFBS match to FuR_variant_1, Ferric Uptake
                     Regulator, confidence: weak, score: 20.71"
                     /tool="antismash"
     misc_feature    9417..9431
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: medium, score: 20.63"
                     /tool="antismash"
     misc_feature    complement(9418..9433)
                     /note="TFBS match to FuR_variant_1, Ferric Uptake
                     Regulator, confidence: weak, score: 19.58"
                     /tool="antismash"
     misc_feature    complement(9531..9547)
                     /note="TFBS match to HexR, Glucose-responsive regulator,
                     confidence: weak, score: 19.54"
                     /tool="antismash"
     gene            9711..9863
                     /locus_tag="A9K65_RS01210"
                     /old_locus_tag="A9K65_001210"
     CDS             9711..9863
                     /codon_start=1
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF033528.1"
                     /locus_tag="A9K65_RS01210"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A9K65_001210"
                     /product="lasso peptide"
                     /protein_id="WP_010913078.1"
                     /transl_table=11
                     /translation="MEQNVEKHEYETPSLTVHGSIETITQGGGGSTAIDASFPAHTPIG
                     DLTFS"
     CDS_motif       9711..9788
                     /prepeptide="leader"
                     /note="peptide class: lassopeptide"
                     /note="predicted sequence: MEQNVEKHEYETPSLTVHGSIETITQ"
                     /locus_tag="A9K65_RS01210_lassopeptide"
                     /aSTool="lassopeptides"
                     /tool="antismash"
     CDS_motif       9789..9863
                     /prepeptide="core"
                     /locus_tag="A9K65_RS01210_lassopeptide"
                     /peptide="lassopeptide"
                     /predicted_class="Class II"
                     /score="-0.30"
                     /molecular_weight="2258.4"
                     /monoisotopic_mass="2257.1"
                     /core_sequence="GGGGSTAIDASFPAHTPIGDLTFS"
                     /aSTool="lassopeptides"
                     /tool="antismash"
                     /leader_sequence="MEQNVEKHEYETPSLTVHGSIETITQ"
                     /leader_location="[9710:9788](+)"
     gene            10001..10315
                     /locus_tag="A9K65_RS01215"
                     /old_locus_tag="A9K65_001215"
     CDS             10001..10315
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lassopeptide: Stand_Alone_Lasso_RRE"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF05402"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019862839.1"
                     /locus_tag="A9K65_RS01215"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A9K65_001215"
                     /product="PqqD family protein"
                     /protein_id="WP_013528114.1"
                     /sec_met_domain="Stand_Alone_Lasso_RRE (E-value: 6.9e-30,
                     bitscore: 101.5, seeds: 25, tool: rule-based-clusters)"
                     /sec_met_domain="PF05402 (E-value: 2.3e-19, bitscore: 68.0,
                     seeds: 461, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNGELSDRDLGDHDSVCATKDAVACEFGDGLALLNLKSNVYYSLN
                     GVGAFIWDLIQEPRSIGDIRSAVLARYNVDPARCQADVDALLKGLADNGLARRHHEALV
                     "
     aSDomain        10043..10294
                     /aSDomain="Stand_Alone_Lasso_RRE"
                     /aSTool="RREfinder"
                     /database="RREFam.hmm"
                     /description="Stand-alone RRE protein in a lasso peptide
                     cluster"
                     /detection="hmmscan"
                     /domain_id="rrefinder_A9K65_RS01215_Stand_Alone_Lasso_RRE.1
                     "
                     /evalue="3.00E-32"
                     /identifier="RREFam002.1"
                     /label="A9K65_RS01215"
                     /locus_tag="A9K65_RS01215"
                     /protein_end="98"
                     /protein_start="14"
                     /score="101.5"
                     /tool="antismash"
                     /translation="SVCATKDAVACEFGDGLALLNLKSNVYYSLNGVGAFIWDLIQEPR
                     SIGDIRSAVLARYNVDPARCQADVDALLKGLADNGLARR"
     misc_feature    complement(10405..10420)
                     /note="TFBS match to ColR, Phenol-responsive regulator,
                     confidence: weak, score: 21.86"
                     /tool="antismash"
     gene            10485..10862
                     /locus_tag="A9K65_RS01220"
                     /old_locus_tag="A9K65_001220"
     CDS             10485..10862
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lassopeptide: PF13471"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF024862.4"
                     /locus_tag="A9K65_RS01220"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A9K65_001220"
                     /product="lasso peptide biosynthesis B2 protein"
                     /protein_id="WP_224618418.1"
                     /sec_met_domain="PF13471 (E-value: 2.3e-17, bitscore: 61.6,
                     seeds: 84, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIFLGYCLLVVATVRLGLTLLSYNRVRSLVTRLDARQCASMGELR
                     RVAWGVAAAARFVPYATCLTQALSGQYILAGQGNESTIRIGIERGTGEQLKAHAWLVSD
                     NHIVLGGSIDGFAHLVDHGSR"
     gene            10859..12781
                     /locus_tag="A9K65_RS01225"
                     /old_locus_tag="A9K65_001225"
     CDS             10859..12781
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lassopeptide: Asn_synthase"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) GATase_7"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1177: asparagine synthase (glutamine-hydrolyzing)"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006205260.1"
                     /locus_tag="A9K65_RS01225"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A9K65_001225"
                     /product="lasso peptide isopeptide bond-forming cyclase"
                     /protein_id="WP_064992950.1"
                     /sec_met_domain="GATase_7 (E-value: 1.3e-25, bitscore:
                     88.2, seeds: 307, tool: rule-based-clusters)"
                     /sec_met_domain="Asn_synthase (E-value: 2.1e-60, bitscore:
                     203.8, seeds: 78, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSGVAGILLRGANAPAVAAADIQQMLARMRHRGPDGSSWWLDTSI
                     ALGHAWLNTSDEVGPGPLTMAGGRLAITADCRLDNRDELLARLGIRDQPVADAIVLMRA
                     YLRWGEACPEHLQGDFAFAIWDDERQSLFCARDHFGVKPFYYHAADRRFAFASEIGPIL
                     GVEGIRARVSERQISGFLAGLPDDPQSTPYSDIFSLPARHSLTVTAQQVMLRRYWQIEP
                     SARPLRSDASEEFAHLFSQSVRNRMRGTSAVGAMLSGGLDSSSIACVAGLQNAAARKPR
                     LPTFSLIFEKGSPMDERPFIDAVLDQQKVDATLIPVGNYAPFAEFERVLEEQEGTFLAP
                     GLTLTRGIYRTAGAKGIKVLLDGHGGDEVVSQGHGHLHELANGGRWMDLWREVRSASNT
                     YGDSTLGLYLQFLTLYGPAWRIARLRHMANRALNKIRKPAQVSRARSWRDLINPDLAKR
                     TELVDRFHRAGYMPPGVQASDALSHRWILSNGYVPHAFEVLDKAAANFGVEPRYPFWDK
                     PLVEFCLALPGEEKLSHGFGRYVLRRAMQGVLPEAVQWRRDKIDFTANLVNGMLRNHRD
                     LLEQLLVSDANRIAPYVNLPQVSAAYARLLRQPDQAAPLDVQYVWRSASLSLWLRQLQH
                     GGSPA"
     gene            12778..13764
                     /locus_tag="A9K65_RS01230"
                     /old_locus_tag="A9K65_001230"
     CDS             12778..13764
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013528117.1"
                     /locus_tag="A9K65_RS01230"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A9K65_001230"
                     /product="serine kinase"
                     /protein_id="WP_084015561.1"
                     /transl_table=11
                     /translation="MMPRNEPLALCNDDAFSHGRAKRERRFYRAYGLTIASEVTLPELQ
                     PAAPGAADVVIAVGAIDLPKPSPEAATDFRFEPDRQYLAWHAVGAFLITDSSRIDIEPA
                     PGVDDALLAFPLLGPIMALLLHRRGLLVLHASAIAAAGTSAIFMGDKGAGKSTTASAMI
                     RAGHRLLTDDVVALDLANPSEPMTVPGFPQIKLAADAAAAISLGEAEVRPQVHPAIDKM
                     QHRLNGSFSGDEVPATRIYILERGERADITPLPAIAALPAIIKFSYVTRFGRAALPGDF
                     ATAHLRQCSWIANHIGVYRLEVPTGLDRIGEAVELIEKDLSAGSRPL"
     gene            13773..15617
                     /locus_tag="A9K65_RS01235"
                     /old_locus_tag="A9K65_001235"
     CDS             13773..15617
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1288: ABC
                     transporter related protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013528118.1"
                     /locus_tag="A9K65_RS01235"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A9K65_001235"
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="WP_064992949.1"
                     /transl_table=11
                     /translation="MSNPPILRLSLFREIAAFGATIARIGGRRTWTALFFLILGSLTEG
                     ISILLLVPLLHLVGRADQDFAVRLPNDDFVRWLVPDGTLQLTTVLCALVGLVAAQAAFN
                     RFKSVYMAKLLFDFINRVRMNLFESIGKARWGVFTRMRSSDLDHALTGDIDRVQGAAFS
                     LLMLVQIAVLLAGYLLVSVFISPVMTLFAVVIGILMFVALRPFRARATAFGRVLTANRQ
                     DQYRTVSEFLGGIKVAKSLNVEDSYFAQLRSTLERMKADNIDYVRNSTIGTAVFQVASV
                     VGLSLFIYVALVRFNLSLAEIVVLLLVFMRIAPRFMDMQTQAQQVLINLPAFTAMRSLQ
                     ARFDAEREPGHTEFADAGTLSLNTGLNIRGVSFAYGDAVDKAVVSGITFGLPAGKVTAL
                     IGPSGSGKSTIADMLLGLLEPTTGKMLVDGVEIDASNRRRWRDQVAYVPQDVFLLHDTI
                     AANLRLAAPRASDGDLWAALRQAHAGEFVERLDLRLETVVGDRGVRLSGGERQRIALAR
                     ALLRKPSLLILDEATSALDWQNQSLIARSIDGLRGTMTILTIAHRPSMIAFADWVVAME
                     NGRIVEVGQYQRLKEKTGSRLSRMLLGEQSESEPADVA"
     gene            complement(15727..16002)
                     /locus_tag="A9K65_RS01240"
                     /old_locus_tag="A9K65_001240"
     CDS             complement(15727..16002)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010913072.1"
                     /locus_tag="A9K65_RS01240"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A9K65_001240"
                     /product="exopolysaccharide production repressor ExoX
                     protein"
                     /protein_id="WP_013528119.1"
                     /transl_table=11
                     /translation="MALPKFILGMIFALAIVVGWSWLGGASIGTILVRVIICAVTIQAG
                     YFVLIYTMIARSAPTPADIARDAERKLNAPEVAESEKLGGARRGLH"
     misc_feature    16277..16286
                     /note="TFBS match to ToxT, Regulator of MSHA biosynthesis,
                     confidence: strong, score: 18.04"
                     /tool="antismash"
     misc_feature    16278..16291
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 18.7"
                     /tool="antismash"
     misc_feature    16279..16292
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 21.2"
                     /tool="antismash"
     misc_feature    16279..16293
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 18.87"
                     /tool="antismash"
     misc_feature    complement(16280..16293)
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 21.72"
                     /tool="antismash"
     misc_feature    complement(16281..16294)
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 17.81"
                     /tool="antismash"
     misc_feature    16457..16471
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 18.06"
                     /tool="antismash"
     misc_feature    16487..16501
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 17.71"
                     /tool="antismash"
     gene            16605..17282
                     /locus_tag="A9K65_RS01245"
                     /old_locus_tag="A9K65_001245"
     CDS             16605..17282
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Bac_transf"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1146: sugar transferase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006205264.1"
                     /locus_tag="A9K65_RS01245"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A9K65_001245"
                     /product="sugar transferase"
                     /protein_id="WP_013528120.1"
                     /sec_met_domain="Bac_transf (E-value: 6e-66, bitscore:
                     219.9, seeds: 461, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRDVAKSATASFSQADIDFPPPIGGLLKRSFDITGSLIGLIALSP
                     LFVMIALLVKFSDGGSIFYGHRRIGRGGRIFPCLKFRTMVPDGDKVLAAYLATNPDANA
                     EWIATRKLKNDPRVTRVGAVLRKLSLDELPQIINILQGDMSLVGPRPVVRDELEIYGTS
                     AVYYLKSRPGLTGLWQVSGRNDVSYDSRVAFDRHYVENWSLFEDVRIIIKTVPAVWMSR
                     GSY"
     gene            17384..18646
                     /locus_tag="A9K65_RS01250"
                     /old_locus_tag="A9K65_001250"
     CDS             17384..18646
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_process="GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Poly_export"
                     /gene_functions="transport (smcogs) SMCOG1186:
                     polysaccharide export protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019862833.1"
                     /locus_tag="A9K65_RS01250"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A9K65_001250"
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="WP_064992948.1"
                     /sec_met_domain="Poly_export (E-value: 7.9e-18, bitscore:
                     62.8, seeds: 183, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MFEAFRGPVSGRLRRARLLATCLAMALVVPHIAAAGDYRLGSQDK
                     LTIRVAEWQTVEGTFRDWSAVNGEYTVGPSGTLSVPFAGELPASGKTTSEIATAIGEAL
                     QKKLALSDRPEASVEMAQFRPFYISGEVQNPGQFPYVPDLTVLKAISVAGGIRRNADYG
                     PQLGKDLVTAKGMFDISDDQRIRLIVRRARIDADMAGKASFDVPKEVEGDPRLPAIVAD
                     EMTILTADQKALKLKLEALDDLKGVLESEIESLQKKIVNQQQQVDLAQQQLASIGPLAQ
                     KGLIANARLLDSRQSVADLQGKILDYETAILTAKQAISKAKQDAIDAQNTLSSSLATDR
                     QQTETDLNEAALKVNMQKGLIAQASDPAMAAAMTNDQQPTLLYSLVRNVDGKTSEIAAK
                     EETPVLPGDVIKIKLAPLASQ"
     gene            complement(18836..20149)
                     /locus_tag="A9K65_RS01255"
                     /old_locus_tag="A9K65_001255"
     CDS             complement(18836..20149)
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0008754 - O antigen ligase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Wzy_C"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013891497.1"
                     /locus_tag="A9K65_RS01255"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A9K65_001255"
                     /product="O-antigen ligase"
                     /protein_id="WP_064992947.1"
                     /sec_met_domain="Wzy_C (E-value: 4.1e-20, bitscore: 71.0,
                     seeds: 81, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKIPKSLLIDPEKNSVYGAFAVAVSIWAFSYSVIFGQILILAYYA
                     VWLPLILVDYRRFVRHLSSAWLPLLFAAYVCFSVFWSQAPGVTARTSVQYFSHIACAYV
                     AARTVSVRTLTIGALIGIFVVLIYSLKVGNYSEDVLDGTVNFVGAFASKNQIGFVGSLG
                     IYFCVVFLAFYRRGRLSFILAVPVVLLSAYVLVASHSATSMASIPAVLALVALLAMSKL
                     LSRRYRRVIFLIGAGLLVVTAFVALNLGLMDFVLGLFGKDSTLTGRTYLWEQGWNTVQK
                     SPILGVGYAAYWVQGFAEAERLWNEFYITTRTGFHFHNTYIEALVELGFVGATLVSLIM
                     LRTLYGHVSAVIFKAWQADSVILFGVMVLLLIRSFVEVEILNPYIMGSFLMYFSFFKLA
                     RLPVTRRRRSPALEPADAAGGETDWPRYAGQPAGAGPS"
     misc_feature    20262..20280
                     /note="TFBS match to FuR_variant_2, Ferric Uptake
                     Regulator, confidence: weak, score: 23.49"
                     /tool="antismash"
     gene            complement(20351..21178)
                     /locus_tag="A9K65_RS01260"
                     /old_locus_tag="A9K65_001260"
     CDS             complement(20351..21178)
                     /NRPS_PKS="Domain: PKS_KR (9-187). E-value: 8.1e-13. Score:
                     40.6. Matches aSDomain:
                     nrpspksdomains_A9K65_RS01260_PKS_KR.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) adh_short"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1001: short-chain dehydrogenase/reductase SDR"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013893279.1"
                     /locus_tag="A9K65_RS01260"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A9K65_001260"
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="WP_027164258.1"
                     /sec_met_domain="adh_short (E-value: 1.6e-31, bitscore:
                     108.3, seeds: 230, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNNLSGRSAIVTGGFSGMGFAIATALAEAGANVAVGSYIAPAGAD
                     RSDAAYYPAADEIERVRSALAAHGTKVHAAHLDVRDSDVTNRFAADAETAIGPADILVN
                     AAGTTAEQPVCGHSDALWDKIVDTNLTGAFRVTRAVLPGMIERGWGRIVNIGSTAALVG
                     WKDNPAYCASKAGLLGLTRCVALEGAAHGVTCVMISPTWVETELMRRNVAQVVEREGKG
                     RTPEEAMAEIARGNPQQRLLQPQEIAALAVFLCSDLAKGITMENIQITGGALW"
     aSDomain        complement(20618..21151)
                     /aSDomain="PKS_KR"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_A9K65_RS01260_PKS_KR.1"
                     /evalue="8.10E-13"
                     /label="A9K65_RS01260_PKS_KR.1"
                     /locus_tag="A9K65_RS01260"
                     /protein_end="187"
                     /protein_start="9"
                     /score="40.6"
                     /specificity="KR activity: inactive"
                     /specificity="KR stereochemistry: C2"
                     /tool="antismash"
                     /translation="IVTGGFSGMGFAIATALAEAGANVAVGSYIAPAGADRSDAAYYPA
                     ADEIERVRSALAAHGTKVHAAHLDVRDSDVTNRFAADAETAIGPADILVNAAGTTAEQP
                     VCGHSDALWDKIVDTNLTGAFRVTRAVLPGMIERGWGRIVNIGSTAALVGWKDNPAYCA
                     SKAGLLGLTRCVALE"
     CDS_motif       complement(21086..21151)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_A9K65_RS01260_0001"
                     /evalue="8.50E-06"
                     /label="PKSI-KR_m1"
                     /locus_tag="A9K65_RS01260"
                     /protein_end="31"
                     /protein_start="9"
                     /score="18.4"
                     /tool="antismash"
                     /translation="IVTGGFSGMGFAIATALAEAGA"
     gene            complement(21266..21760)
                     /locus_tag="A9K65_RS01265"
                     /old_locus_tag="A9K65_001265"
     CDS             complement(21266..21760)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013528124.1"
                     /locus_tag="A9K65_RS01265"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A9K65_001265"
                     /product="acetone carboxylase subunit gamma"
                     /protein_id="WP_064992946.1"
                     /transl_table=11
                     /translation="MTSYSKEVLGDLAAGKLPWPQTRRIMSAYKDDDRFFKMVAVYQDR
                     VAWKDPILLPVSDHLFICQSGDERITRCECGHSFGDYRKNWKLSAVINVRNTEEALREI
                     YPNSDLPDPEWMEIREFICPECGTLHEVEAAAPGYPIVYDFEPDLEGFYREWLGRPLQG
                     S"
ORIGIN
        1 acttcacctt tgccgccgtg ttcggccttg agcccctgca ccaggaaacc gacctcgtct
       61 tcgaggtcgt gccgcatccg cgcatgttcc agtggaactt catgcatcac aaattcctgg
      121 cgcaatgctg cgtcgacatt gacggcgtgc tgtgcctcga cccgacagag gcggaaaacg
      181 acgacggccc ggcctatgag aaattcctca gcgaagcgcg gccgctccat gtcgcgaccc
      241 acaggatcgg ctggctggtc accagccggc tggagaaata ccgcgcgctc accgaggcat
      301 ggctcgccaa gcatgagatc aaatacgacc agctgatcat gctcgatctg ccgagcaagg
      361 cggaacggca acgactcggc gcgcatggca gtttcaaggc cgatttctac cgcaagtccg
      421 atgccatcct gttcatcgag agcgagcacg aacaggcgct gaagatcacc gaactgtcag
      481 gcaagccggt gctgtgcgtc gaaacgcata tggtcagctt ccccgacgcg ctgtcgctgc
      541 cggcacttgg ccaggcggcc cgcaacctgc cggcaaggct gcggcagatc aagtcgcaag
      601 agggccgcaa gagcgccgcc aagaccattg cccgcgcact gcttggcgcc cgcggctacg
      661 agacgctgaa aagccgggtc aagcgtccgg cctgacctgc tcaggcgcgt cgtcaggccg
      721 cctgtcgcgc acgctgctgc atggccctgt ccagtatcga gaagaaggtg atgaactggc
      781 gatcgggatc gagttccggg cgcccggcga agctgcgagc gagactggaa agccggtcat
      841 attcctggtt gtcgctccac agccgcctga cggccgcgac ccaatcggcc agcggagcat
      901 cgtaatccag caccactcca ccggagccga cagcctcggg cagaccgccg cgccgcgaac
      961 cgataacggg gattccgctg caatgggcct ccgaagcgac gcggccccac gcctcttccc
     1021 acttgctggg agcaagcagg atcttcgtgc ggccatagac cgtcttcatg tcgctggtgc
     1081 ggctctccag gacgacattg cccaagggag cgatcgtctg ttcgatccgc gcccggtgat
     1141 cgtcgtcgag cttccagctt tcgacgaaca ggaatggaat ttcgcggcag gcgccggcaa
     1201 tccgcaccgc gagctcaaat cccttttcct cataggggtt gatcagcgtg acgaactggc
     1261 ccgtcgtcgg cgtcgagtaa tgcgccgggt tgatggttgg cgggatcact gctgcctcga
     1321 tgccgaattc ttttttgtag gttcgcgcgg tgaaatccga attggcaatg taaagcgccg
     1381 aatgcaattc gcgcagatcg ccgcccagtt catggaactc gacattgcgg agataaacga
     1441 ccagaggcac gccctccgcc tgcagcgcct tgccgatcgg aaccgatttg tgacactgga
     1501 ccacggcgac atcgggcttc agcctgtcga cggcataacc cgccgcttcc cagggaaacc
     1561 aggcccgcac caccggatag ccgggatagc tgtcgacaac cgccggctgc cgcaacagct
     1621 tcatcttggc acgcgccttg aagccgaaga caccgtcacc gaacagggcg gcgagcacag
     1681 cggcttcatg accgtgctcg atcagctgtt cggccagatg atgcgtgctc gactgaacgc
     1741 cgccactgaa ctcgggcggg tagccgttgc cgcctgcaaa gagaactttc atgatcgggc
     1801 tccttgtcgc atctcgtatt ccttgctcgg agacaacagg caaaggccgc cgcctcgctt
     1861 tcgacgtcag gcaagtttcg gcggctccag attggcgaag ggattggtac aggattgcca
     1921 gccggtaaac cggatcgggt cgttgggcga actgcgccag gcgtagtccg tcagcatatg
     1981 gaattcggcg acgacccagc ggtcgaaatg cagcagatct tcccgcttcc gccggggcag
     2041 caagcgacgc gcggcgccca gcttgagctc ctccaccagg gtgacggcgt cactcagttt
     2101 ttcgcttgga aagttccatg gattccggtc gcggaacgcc agcacgaact gctgcagatg
     2161 ctcgatgcgg atgttcaact tgccaaggta tttttccagc gtgacgcgca ccagatcctc
     2221 gtccgagaag gaggaaacgg tggcgtaggc gacccctgtc gagaccactc cgccagccaa
     2281 gacggccagg gccgagcgtc tggaaatctc gatcatgcga tacctccttt cacgcgatcc
     2341 ggctcgccgc ccgcaaggac agcgctgccg cggtcaggct cgggttggcg caggagcagc
     2401 tcggataggt gctggtgccg acgacgacca ggtttctgag ctgatggtgg atcatgccgc
     2461 tgtcgaccac agaatcggcc gggccggtcc ccatcctcag cgtgccctgg acgtgcgatt
     2521 cggtcggccg aatgccgcgg tcgaagagct tttcgaccgg cagcggcgcc agaaggtcag
     2581 gcaatttctc cagagcgcgc gccatgccct tcaccgcata gtccgatggc gccttgaagc
     2641 tgacgaaggc attgtcatcc gcgtcaagcg tgacgtagtt ctcggggtcg agcaggttct
     2701 cggtgaccac gaccaacggc agcgcctggc gtagccgccc cttttcggca cgcattccgt
     2761 gctgccagcg gttctcgaaa tagaccagtg ccgccgcatg ctccgatcgg tgaggaccat
     2821 catagaggcc gaaattcagg cctgttgtga tggtgctgcc gtcgaaattg tcgacgccgt
     2881 cgagatagac ctcgaaattc cagccgtagg attcatgcag gccgctgccg acaaactcgc
     2941 cgcccaggcc ggatcgcagc atgatcgccg cgctctggat ggcattggcg ccgagaataa
     3001 agagatcgcc gctgacctgg tattccttgc cgtcatggct gaacgtgacc gaacggacag
     3061 cgccgccgac gtgatcgagc cggcgcactt ccgcaccgag gcagaccgag atgtcgggat
     3121 gctcgaagac gtgcatcaac ccgttgttga cggtgaattt ggcgtcgacc gggcacagcc
     3181 agcaacgcag attggcacag cacgagctgc gctgcgcggt cggcacgcga gcccgcgcgg
     3241 ttggcatgac gaagtgctgg tcgggctgcg ccgccttcat catcctgtcc ggtgtcgaca
     3301 tgcgatgcgg cggttgcgga aacggcttcg agcggggcag catcgccgcc atgtcggggt
     3361 cgccggagat cgacatgatg tcttccgcgt cgcagtagaa cggctcgacg tcgtcataac
     3421 tgatcggcca gtcattgccg atgccgtagg tgcttttcag ccggaaatca ttgggatgaa
     3481 atcttggcgt ctgcgcgaac cagcagttcg tcccgccacc cagtccgatc gtgtagttcc
     3541 acggcttgtc ggaattggtc ttgtaggtcg tctcgtcttc gatgtcggtg ttgacgttct
     3601 gttcgagctg ccattcatgg gtgttgtggc gtccccactc cagcaccaga atgcgcgcct
     3661 ttcggcgcct ggcgaactca tgcaggaaga aggccgaacc gaagccggaa ccgatcgcga
     3721 cgagatcgaa atgattcttg gtgatttgct ccggctttac gtccagcacc atcagctaca
     3781 actccacaca gaaatacgcg ctcaacccca gcgcccgcag gccgtgtgcg gaccggtctt
     3841 gcatccggct gccagggccc ggtctcatgc cgccattctg ccgatcgagt gatattcgat
     3901 gccgtggcgg gcgatgacct tggggtcata gaggttgcgg ccgtcgaaga tgaccggcgt
     3961 ggtcagcgcg tccttcaccg cgtcgaagga cggcgcgcga aagctcttcc attctgtggc
     4021 gatcagcagc gcatcggcgc cgcgaagcgc cgcctccttg gtgccgcaca gaagcaggtc
     4081 gtcgcgcagc ccgtagatgg cctggcattc ctgcatcgcc tcgggatcat aggcctgcac
     4141 attggcgccg gccttccaga gcgcctccat caggacgcgc gccggcgcct cacgcatgtc
     4201 gtcggtgttg ggcttgaagg ccagacccca gagcgcaaag gtcctgcctt tgaggttgcc
     4261 cttgaaatag cggtccacct tgtcgaagag aaccgatttc tgtgcgttgt tgcgctcctc
     4321 aacggcgcgc agcagcttgg cgtcgaactt gacgccttcg gcggtcttga tcagcgcccg
     4381 cacatccttc gggaagcacg agccgccata gccgaggccc ggatagatga aatggtagcc
     4441 gatgcgcgga tcgctgccga tgcccttgcg gacctcctcg atgtcggcgc cgagttgttc
     4501 ggccagattg gccatctcgt tcatgaagct gatcttggtc gccagcatgc agttggcggc
     4561 atatttggtg aattctgcgc tgcgcacgtc catcacgatc atcttctcgt ggttgcgatt
     4621 gaacggcgcg tagagttcgc gcatcacagc ctcggtgtcc tcgctgtcgg tgccgacgat
     4681 gatgcggtcc ggcttcatgc agtcggcaac cgcggaccct tccttgagga attccggatt
     4741 tgaagcgaca tcgaaagcca ggtcctcgcg gcctcttttc ttcagcgtgt cggcgatcct
     4801 ggccttcacc ttttcgcagg tgccgaccgg caccgtcgac ttgccgacga cgatcttggg
     4861 tttgtccatt tcgcggccga tggtctcggc gactgccagc acatatttga ggtcggccga
     4921 gccgtcctcg cccggcggcg tgccgaccgc gatcatctgg atctcgccgt gcctaacagc
     4981 ggcggcggca tccgttgtga acttgatgcg gccggcggca tggttctcct taacgaggtt
     5041 ttccaaaccg ggttcgaaaa tgggaaccag gccctggttg agccgctcga ccttggcggc
     5101 gtcgatgtcg acgcacacca cctggtgccc gacttcggca agcaccgccg cctgcacgag
     5161 gccgacatag ccaattccaa acaccgtcaa attcattcgg tttcattccc atgcaaggca
     5221 gcggaccgtt ttcggccccg ccggttcaaa tcgactttcc caactacttt acactgcatg
     5281 ccagcgattc cggaaattga cagggttcgc cgagcgccgt gaaggcgacc cgctcgacct
     5341 gcagggaaag cttgcggccc ggatccgcga acgctcccat ccagcccttc atcgtatcgc
     5401 tgccccaaaa gctgaagaat atcttctgtg catggctggg cagcttcgcc gggtcggtaa
     5461 tcgtgttgac cagttggccg ttgacgtacc atcgcaagct gtccttctgc cagacgaagg
     5521 cgtagtcgtt gaaggccttc tccgtgccgc ccggcacgtc gaccagtttc tcgttcttgc
     5581 ccttgcccgc gatataggcg ttgacctgca ccttggaggt gtccttggtc aggacctcga
     5641 agtcgatctc gtcccagggc tgcttgtcgc ttggaccgat ataggtgaag aacgcggcgt
     5701 tgaggccgga gccggtatcg gtcttcagcc gtgcctcata ggtgccatag ccgaagcgct
     5761 gcttggtctg gatctcgccg cagacgtagt cgcgatcctt cagcgtctgc ttttcgaagc
     5821 cgagcgacag cacaccattc gacagttcga ccatattttt cgaccaggtg caattctgat
     5881 ggctgccgtt gttccagccg tcggaaacat accagcgcgt gcggtcgaag cttttgaaat
     5941 catcgacaaa ggacggcgat gtcggaatgt cctgggcatg ggaaggggtt gccaggaaac
     6001 cgccaagagc agccagcagc aacgcaccga gcgaccccag ccgccgggcg gcttgtccgg
     6061 tccgatatgc gagaggcggt gcgtcaattg ccgtcgtagg tttcggattc caattcatac
     6121 caaactcacc tttgcgctgt gtgtccccaa cccaaagtcg acgttcgaac cgctcctgca
     6181 cgggccaacc ttgaccttcc ttgtgaccct ctcgattgct tctgcttccc gccttgcatc
     6241 aaatctgttg caacagttcc gagccttcac gttcaacctc tgccggcgcc tgccggccag
     6301 gcgaattccg ccgcggcggc cgaggaggca tcctcgtcaa agaccgtatc cagcgaatga
     6361 cggagctctt tcatcatgcc gtcctggcgc gacagcgccg cgatgcggcg ctcgcaattc
     6421 aggatcgact gtgtcagggc gctgacttcc gaagaccgct tgtcggaagc agagccgttt
     6481 tccatcgctt cgaccagaaa ggcggcgttg gtcgaggtcg tccggtagcg attctcaagc
     6541 ccggttctct cggcctcgat ctcggccgca atctgctcga acagctttgc cagacgatca
     6601 agacgtgaaa catcggcctg acggtcgcgg gccggatccc ttgatctgaa accgaatatc
     6661 gaggccatga actcaggttc ccgtcatcgc cggcttgtgg gccgggcttc tggccgatcc
     6721 ccgcagccgg ggctcaaaaa ctcgtgttgc gttgtcgtgc tccttttgag ggagagacat
     6781 cagaccaatc cgatcggggg ccaagttcgg ctggtcgtcc actatgctgc accgcaacat
     6841 agactgccat cgtcggagcc gcgaggcaac cacccaattc gtaaactcat aaatcggccg
     6901 aaatggtttg cgtgatgaca tgccgcgagt ggacaaatcg ctgcatcggg accgctctca
     6961 gcgttccttg cccgtgctgg ttccggccat cagaaagcgc agcggcggca caggcttttg
     7021 gctggacaca aggccgacac gacgaaacag cgcctcgagc ttgtcggaag ccacgccctg
     7081 gacgatgggg acgaggttgg acaagctggc gaaggcgtaa gccgcagccg agcccagccc
     7141 ccgctcggcc ttgacgtcaa ggaaattacg cagccgatat ttgtcgcgca cgtgccgttc
     7201 atgccgccgc agcaccgcct tcgagccttc cggcaggctg tcgatcgcca gcagcgccag
     7261 atccgcatcg gccaaacgct tcaagtcctg ggtcttgtgg cggccgctca gggaatcggc
     7321 gcggacgatg gcgccgtagc cgcaggagcg aatgaccttg aaccgcgccc cgcatgcgac
     7381 cgcgcgggcg tagagttcat agtcctcgcc cagccgcagg ctctcgtcgt agctcaaccc
     7441 atggcggtcg aggaaggcac ggctgatcac cggcttcaaa aagcccagtt cgcccctctg
     7501 cacgcggcgc ctggagatgt tgccttcgac gaaacgctcg aaatcgagga attccggatc
     7561 ggcggcaaat tgcggcgcga cgattttcgt cgcgtcgccc gtcgcatcat ccctgatcag
     7621 catgatgttg tcggcggcga aatcccaatc ggcgccggca aacaggttgc gaaaccggcc
     7681 ttcgaggaag aaatcgtcgg catcgagaat gctgatgaac ggcgatttcg agccggcgat
     7741 ggctgcgttg cgggcaaagg aggggccgcg attgacatcg aggcgcatca cggagagccg
     7801 cccgctgcca tcatcggccg atcgtgcgac accggcggtg tcgtcggtgg aggcatcgtc
     7861 gacgaccacg acctccgcca cttccggttc gcgcaatgcg gatgcgatgg cgacggcgat
     7921 tgtcgctgcg gcgttttttg ccgcgatgat cacgcagacc tcggatttcg tcatcgacat
     7981 ggatttgcct ctcgtacagg taatatcgat ttgccctcgc cccgtactcg ttcgatgcac
     8041 tacagaattg ccatcagcat ccaatttacc ttcagcgact cgccggccgc tcgaagatgc
     8101 ggcggctgat atcggcggac gccgcccagc ctgcttcggc cagatagcgg accggcgcgc
     8161 ggccgcacag ttcccaaagc accgttcgcc gctgcggcca ccgcagcgac ggcagccacg
     8221 gcgcgatgac catgctgagc agatcctcat tgccgatgcg cggcaggatc ctggtcgcgc
     8281 gctgcgacag cagcgtgccg gcaccgccga gtagcacgtc ggcggcgcgc aggccaagca
     8341 gcagcgtgtc gtccagacct tgcgcggtag cggcatcgag caaacgctgc tgatccgcct
     8401 cgtcgaggcg gccggcgctg gcgccgatat cacagacgta tccggcgcag ggttcccggt
     8461 tgaaaagtgc cttggcgacg ctgatgcagg acaaaagcaa tgtatcggcc accgaggtga
     8521 acggcaccgt cgcaccggcg atttcgacct gtcgcttgcg ccgcaggaac ccgtccgcgt
     8581 cgcgcgggct tggcgagccg ggctgctgga ggcggtagtg gagatcgacg gtggacgacc
     8641 tcggcccgtc gtcccgggtc atgtgctgtt cgccgaggaa gcggacccac cagacggagc
     8701 tcgacttgcc tgatacctca tagccgattt tgcgaagcgc atcacgcgcc ctgaaaaatc
     8761 cgagcggcga aaccaggatg tcgacatcgc ccgagggctt cataaaatgg tcgtcgtaga
     8821 gaagttgctg ctggaacgga cccttcagaa aaacgaaatc gatctcgctg gcgcgcagca
     8881 cgtcctggat cgccatcgaa tccatgatgc aggacgcgtt catcgaaacc gttttcctgc
     8941 ggtaggtgtc gagccattgg aaaagcttag ttggcctctg aagcgcgggt gcgcgcgaaa
     9001 gcgccttgag gacaaagacg gcgaccttgt tcagcctggc aatctcggca attttttcac
     9061 cgtcaagatg gcgcatcgaa acgtcgcccg cgcccgggcc ggcgaaaaac aatcgcaggc
     9121 aagcctgcac ataggccacc tcgccggcac agccggcggc gcgcaatcgt tcgtatgagc
     9181 catccggcaa ggccatttag cgcacagtct cccaagaaag cgtttcgcgc cgacacttat
     9241 gctgcaagtg cgaagcaagc atcaccaaaa acctcgatgc cgtaaagcct ttccttctgc
     9301 agtgcgaatt tttgccacca aagcgtctat cgaggtggca tcggaaagca gtgaaaaccg
     9361 cggttgaaat gtcccttgcc acgtcggctt aggcaatctc cggacgattt ctcaaatgga
     9421 aattcttttc attagagagg cctaaatata ggcacaacgc aaacggctta tgcaagcaaa
     9481 atcaaatgct taaagaatca gcacaggcgc gatcgtcgtg cacaaggaat cggctaaaaa
     9541 atgacaagtt ttaggcatgc cgctcatgtg acgacgccag caaccgtaat tgattaacag
     9601 aaagtaaaac agtcttgact cataaatgct gccgtgcagc tattgcattg cagcataaaa
     9661 gtgctgacgg cactcgacag gccgtttcca gtcctaaatc ggagagtaaa atggaacaga
     9721 atgttgaaaa gcatgaatat gaaacgccaa gcctgacggt ccatggttcg attgagacca
     9781 tcacccaggg cggcggcggc agcacggcga tcgacgcatc gttccccgcg catacgccga
     9841 ttggcgacct gacgttctcc tgagaactac agccgattcc agagtgacat gttgggactc
     9901 tggattcaaa agcagacgta agaggggtcg gggggccagc ctcccgattt ctcctgcgcc
     9961 acctggagat gtcgcagcgt tttgtcagcg aggtttgggg atgaacgggg agctatccga
    10021 ccgtgatctg ggcgaccacg atagcgtgtg cgcgaccaag gatgccgtgg cttgcgagtt
    10081 cggagatggc ttggcgctgc tcaatctcaa atccaatgtc tactacagcc tgaatggcgt
    10141 cggcgccttc atctgggatt tgatccagga accccggtcg atcggcgaca tccgcagcgc
    10201 cgtgcttgcg cgctacaatg tcgacccggc gcgctgccag gccgatgtcg acgcattgct
    10261 gaagggcctg gccgataatg ggcttgcgag acggcatcat gaggcacttg tctaagccgg
    10321 tccagcacaa gtccctggtt tcacgggccc tggtttcgcg ggccttgtct tcgcgggcca
    10381 cggctttacg gcaggaaccg gcgagagaaa caaacgtcaa gcagacgaag cgcggaggaa
    10441 gccggcgcgg gctggtcagg attttctccc tgagtggctc ggaaatgatc ttccttggct
    10501 attgcctgct ggtggtggcg acagttcggc tggggctgac gctgttgtcc tacaatcgtg
    10561 tacgcagcct ggtgacgcgc ctcgacgcgc ggcagtgcgc ctcaatgggc gaactgcggc
    10621 gtgtcgcctg gggcgttgcc gccgcggccc gtttcgtccc ttacgccacg tgcctgaccc
    10681 aggcgctctc cggccagtat attctcgccg gccaaggcaa tgaatcgacg atacgcatcg
    10741 gcatcgaacg tggcacaggc gagcagttga aagcacatgc ctggctggtc agcgacaacc
    10801 atatcgtgct tggcggatcg atcgacggct tcgcccatct cgtcgaccac ggcagccgat
    10861 gagcggcgtc gccggaatcc tgttgcgcgg agcaaacgca ccggcggtcg cggcggccga
    10921 cattcagcaa atgctggcgc gcatgcgcca tcgtggtcct gacggcagtt cttggtggct
    10981 ggacaccagc atcgcccttg gccacgcctg gctcaacaca tcggatgaag tcggtccggg
    11041 ccctttgacg atggctgggg gccggctcgc catcaccgcc gattgccggc tggacaaccg
    11101 tgacgagttg ctggccaggc tcggtatccg cgaccagccg gtagccgacg cgatcgtgct
    11161 gatgcgggcc tatctgcgct ggggcgaagc ctgcccggag catctgcaag gcgatttcgc
    11221 cttcgccatc tgggatgacg agcggcaatc gctgttttgc gcgcgcgatc attttggcgt
    11281 caagccgttc tactaccacg cggcggaccg gcgttttgcc tttgcgtcgg agatcggtcc
    11341 gatcctgggc gtcgagggta tccgcgcacg tgtcagcgag cgccagatct ccggcttcct
    11401 ggccgggctt cccgacgacc cgcagtcgac cccctacagc gatatattca gcctgcctgc
    11461 ccgccacagc ctaaccgtca ccgcgcaaca agtgatgctg cgccgatact ggcagatcga
    11521 gccgtcggcg cggcccttgc ggtcggatgc atcggaggaa ttcgcccatc tgttctcgca
    11581 atcggtgcgc aaccgcatgc gagggacctc ggcggtcggg gcaatgctga gcggcggcct
    11641 cgattcctca tctatcgcct gtgtcgccgg cctgcagaat gccgccgccc gcaagccgag
    11701 gctgccaacc ttctcgctga ttttcgagaa gggctcgccg atggacgagc ggcccttcat
    11761 cgatgccgtt ctcgaccagc agaaggtcga cgcgacgctg atcccggttg gcaactatgc
    11821 cccatttgcc gaattcgagc gcgtgctgga agaacaggaa ggaacgttcc tggcgccggg
    11881 cctgacgctc acccgcggca tctaccggac tgccggcgcc aagggcatca aggtgctgct
    11941 ggacggccat ggtggcgacg aggtcgtttc acaaggccac ggccacctgc acgagcttgc
    12001 gaatggcggc cgatggatgg atctgtggcg cgaggtccgc agcgcatcca acacctatgg
    12061 cgacagcacg cttggccttt atctccagtt cctgaccctc tacgggccgg cctggcgcat
    12121 cgccaggctg aggcatatgg ccaaccgtgc cctgaacaag attcgcaagc ccgcgcaagt
    12181 gtcgcgcgca cgaagctggc gcgacctgat caatccggac ctggccaagc gcaccgagct
    12241 tgtcgaccgg tttcaccggg ccggctacat gccgcccggc gtccaggcca gcgatgcctt
    12301 gagccatcgc tggatcctgt cgaacggcta tgtcccgcat gctttcgagg ttctcgacaa
    12361 ggccgccgcc aatttcgggg tcgagccgcg ctaccccttc tgggacaagc cgctggtcga
    12421 gttctgcctg gcgctgccgg gcgaggagaa gctgagccac ggttttggcc gctatgtgct
    12481 gcgccgcgcc atgcaaggcg tattgccgga ggcggtgcag tggcggcgcg acaagatcga
    12541 cttcactgcc aatctcgtca atggcatgct gcgcaaccat cgcgacctcc tggaacagct
    12601 gctggtctcg gatgcgaacc gcatcgcgcc ttacgtcaat ctgcctcaag tgagtgctgc
    12661 ctatgcgcgg ctgctgcgcc agcccgatca ggcagccccc ctggatgtcc aatatgtctg
    12721 gcgctcggcc tcgctgtcgc tctggctgcg gcagctccag catggcggga gccccgcatg
    12781 atgccgcgca acgagccgct cgctctatgc aatgatgatg ccttttcgca tggccgggcc
    12841 aagcgagaac gccgcttcta ccgggcctac ggcctgacca tcgcgtcgga ggtgacgttg
    12901 cccgaactgc agccggcggc gccgggggcg gcggatgtcg tgattgccgt cggcgcgatc
    12961 gacctgccaa agccctcgcc cgaagccgcg accgactttc gcttcgagcc ggaccggcaa
    13021 tatctggcgt ggcatgccgt gggcgcgttc ctgatcaccg attccagccg tatcgacatc
    13081 gaaccggcgc ccggcgtcga cgacgcgctg ctcgcgtttc cgctgcttgg tccgatcatg
    13141 gcgctgctct tgcaccggcg cggcctgctt gtcctgcatg ccagtgccat cgccgccgcc
    13201 ggcacgagcg ccatcttcat gggtgacaag ggagccggca agtcgacgac cgcaagtgcg
    13261 atgatccggg ccgggcatcg actgcttacc gatgacgtgg tggcgctgga cctcgccaat
    13321 ccgagtgagc caatgaccgt tccgggcttt ccgcagatca agctggcggc cgacgccgcg
    13381 gcggcgatct cgcttgggga agcggaagtg cggccgcaag tgcatccggc gatcgacaag
    13441 atgcagcatc gcctgaacgg tagcttttcg ggcgacgagg tgccggcgac caggatctat
    13501 atcctcgagc gcggcgaaag ggctgacatc acgcccttgc ccgccattgc cgccctgccg
    13561 gccatcatca aattctccta tgtgacgcgc ttcggccgcg cggccctgcc cggcgacttc
    13621 gcgacggcgc atcttcggca atgttcatgg attgccaacc atatcggcgt gtaccggctg
    13681 gaagtcccga cgggcctgga caggatcggc gaagccgtgg aactgatcga aaaggatctg
    13741 tcggctggca gccggccgtt gtgagcgcaa ccatgtcgaa tcccccaatc cttcgcctgt
    13801 cgctgtttcg agaaattgcc gccttcggcg ccaccattgc ccggatcggc ggccggcgaa
    13861 cgtggacggc gctgttcttc ctcatcctcg gcagcctgac cgagggcatt tcgatcctgc
    13921 tgctcgtccc gctgctgcat ctggtcggac gtgccgacca ggatttcgct gttcggctgc
    13981 ccaatgatga tttcgtgcgc tggctggtgc ctgatggcac gctgcaactg acgaccgtgc
    14041 tttgcgcgct ggtcgggctt gttgcggcgc aggcggcgtt caatcgcttc aagtcggtct
    14101 acatggccaa attgctgttc gatttcatca atcgcgttcg catgaacctg ttcgaaagca
    14161 tcggcaaggc gcgctggggc gtttttacac gcatgcgcag ttccgacctc gatcatgccc
    14221 tgaccggcga catcgaccgc gtccagggcg cggctttctc actgctgatg ctggtgcaga
    14281 tcgccgttct tctggccggc tatctcctgg tttccgtgtt tatctcgccg gtcatgacgc
    14341 tgtttgccgt ggtcatcggc atcctgatgt tcgtggcgct gcggcccttc cgggcacggg
    14401 ccactgcctt cggccgtgtg ctgaccgcca atcgccagga tcagtaccgg acagtctcgg
    14461 aatttctcgg cggcatcaag gtggccaaga gcctgaacgt cgaggacagc tatttcgcgc
    14521 agctgcggtc gacgcttgag aggatgaagg ccgacaacat cgactatgtg cgcaacagca
    14581 ccatcggcac tgctgtattc caggtggcga gcgtcgtggg cctgagcctg ttcatctatg
    14641 tggctttggt ccgcttcaac ctgtcgctgg ccgagatcgt cgtcctgctc ctggtcttca
    14701 tgcgtatcgc gccgcgcttc atggacatgc agacgcaggc ccagcaagtg ctgatcaacc
    14761 tgcccgcctt tacggcaatg cgcagcctgc aggcacgttt cgacgccgaa cgcgaacccg
    14821 gccataccga gttcgccgac gccgggacac tctccctcaa tacgggattg aatattcgcg
    14881 gcgtgtcctt cgcctacgga gatgctgtcg acaaggcggt ggtgagcggc atcaccttcg
    14941 gcctgccggc cggcaaggtc accgccctga tcgggccgtc cggatcgggc aagagcacga
    15001 tcgccgacat gctgcttggc ctgctggagc ccaccactgg caagatgctt gtcgatggcg
    15061 tcgagatcga tgccagcaat cgccgtcgct ggcgcgacca ggtggcctat gtgccgcagg
    15121 acgtgttcct cctgcacgac acgatcgcgg cgaacctgcg cctcgccgcg ccacgggcca
    15181 gcgatggcga tctgtgggcc gcgctgcgtc aggcgcacgc cggcgagttt gtcgaacggc
    15241 tcgacctcag gctcgagacg gtggtcggcg atcgcggcgt gcggctctcg ggcggcgaac
    15301 gtcagcgcat cgcgctcgcg cgcgcgctgc tgcgcaagcc gtcgctgctc atcctcgacg
    15361 aggcgaccag cgcgctcgac tggcagaacc agtcgctgat cgccaggtcg atcgatggat
    15421 tacgcggtac gatgacgatc ctgaccatcg cgcaccggcc gtcgatgatc gccttcgccg
    15481 actgggtggt ggcgatggaa aacggccgta tcgtcgaggt cggccaatat cagcggctga
    15541 aggaaaaaac cggcagccgg ttgtcgagga tgctgttggg ggaacagtcg gagagcgaac
    15601 ccgctgatgt ggcgtgagcc ggcgaccaag acgcgtcgcg ccgagacgga ttcacgcggc
    15661 gctttggtcc ctttgtctcg aagcatgtcg ttgccgccaa accgggccac gtttgagcag
    15721 tctgcatcaa tgcaggccgc gcctggcacc gcccagcttc tcgctttcgg cgacctcggg
    15781 tgcgttcagt ttgcgctcgg cgtcacgagc tatgtcggca ggcgtcggcg cgctcctggc
    15841 gatcatggtg tagatgagca cgaaatagcc ggcctgaatg gtcacggcgc agatgatgac
    15901 acgcaccaat atcgtgccta tcgacgcacc gcccagccag gaccagccga cgacgatcgc
    15961 cagagcgaaa atcatcccga ggatgaattt tggaagtgcc atacccaaca gcccataaac
    16021 cggctcgggt attatcgcga ttgccccacc cgagcgccgt cccttgtcat gtgggcttgt
    16081 taaggtcgcc cactctcgac cggttgcttc cggccttttc cacctgtcgt caccgtgaca
    16141 ggtataattt gcaattctat taaggcgagc aataggcggc aagggcaact tcggttattt
    16201 ttgatcgagc ttttgcgatc gactctgcca tgttgcaatg cacgcaacgg gagctaggcc
    16261 gatatcctct cgtcgatatt tttttagtaa aattggcaat attaagtagt actttatacg
    16321 gttttgacca agcatcacgg catgatgaag cactaatttg tacctcgcct tggcaaatag
    16381 gtaggtccgc ctaaaaaccg ccccacaaag gcctctattt tttcggccgt gcccatttat
    16441 tgtcacaaat gtgctaaata tgccgaaatt tgtgcatagc aagcttatta tttagtcatt
    16501 tcatgacgtg actatttcaa cggggtggta aattgtgtgt tgcgctgcaa cattttttgg
    16561 gtatcgtgtg gtgaaccatt cgttcaacgc atggagttta cggcatgagg gacgttgcca
    16621 agtcggccac cgcgagcttt tcgcaggctg acatcgattt tccgccgccg atcggcggcc
    16681 tgctcaaacg cagcttcgat atcaccggtt cactgattgg cttgatcgcc ctcagcccgc
    16741 tatttgtcat gatagcgctg ctcgtcaagt tttccgacgg cggctcgatt ttctacggcc
    16801 acaggcgaat cgggcggggc ggacggattt ttccgtgcct gaagttccgc accatggttc
    16861 cggacggcga caaggtgctg gcagcctacc tcgccacaaa cccggacgcc aacgcggaat
    16921 ggatcgcgac ccgcaagctg aagaacgacc cgcgcgtcac ccgcgttgga gcggtgctga
    16981 ggaaactcag cctcgatgag ttgccgcaga tcatcaacat cctgcagggc gatatgagcc
    17041 ttgtcggtcc gcgtccggtt gtgcgcgacg aactggagat ctatggcaca tccgccgtct
    17101 actacctgaa atcgcgtccg ggcctgaccg gcctgtggca ggtcagcggc cgcaacgatg
    17161 tgtcctatga cagccgcgtc gccttcgacc gtcactatgt cgagaactgg tcgctgttcg
    17221 aggacgttcg catcatcatc aagaccgtcc cggccgtctg gatgtcgcgc ggttcttact
    17281 gaccggccgg tggccaatgc cagacattgg cgtcggccca ttcgacgcaa gctcatcggg
    17341 cgatggggct atagcggatc ggaacgttga gttgaaaaca gatttgttcg aagcctttcg
    17401 cggcccggtt tccggccgcc tgcggcgggc gcgtttgctg gccacatgcc ttgccatggc
    17461 cctcgtcgtc ccgcacatcg ccgccgccgg cgactatcgc ctcggctcgc aggacaagct
    17521 caccatccgc gtcgccgaat ggcagaccgt tgaaggcaca ttccgtgact ggtcggcggt
    17581 gaatggcgag tacacggtcg gtccatccgg cacgctgtcg gtgccgttcg ccggcgaact
    17641 gccggcctcc ggcaagacca cgtccgaaat cgcaacggcg atcggcgagg ctttgcagaa
    17701 aaagctcgct ttgtcagaca gaccggaagc ctcggtcgag atggcgcagt tccggccatt
    17761 ctacatttcg ggcgaagtcc agaaccccgg ccagtttcct tatgtgcccg acctgacggt
    17821 gttgaaggcc atcagcgtcg ccggaggcat ccggcgaaac gccgactacg gcccccagct
    17881 cggcaaggac ctggtcaccg ccaagggcat gttcgacatc tccgacgacc agcgcatccg
    17941 actgatcgtc aggcgcgccc gcatcgatgc ggatatggcc ggaaaggcga gtttcgacgt
    18001 gccgaaggag gtcgagggcg atccgagact gccagccatc gtggcggacg agatgacgat
    18061 cctgacagcg gaccaaaagg cgctgaagct gaagcttgaa gcgctcgacg atctcaaagg
    18121 ggttttggaa tccgagatcg aatcgctgca gaagaagatc gtcaaccagc agcagcaggt
    18181 cgacttggcg cagcagcagc tcgccagcat cggcccgctg gcgcagaaag gcctgatcgc
    18241 caacgcccga ctgctggatt cgcgccagtc ggttgccgac ctgcagggca agatcctgga
    18301 ctacgaaacc gccatcctca ccgccaagca ggcgatcagc aaggcgaagc aggatgctat
    18361 cgatgcccag aacaccttga gttcaagtct cgccactgac cggcagcaga ccgaaaccga
    18421 cctgaacgag gccgcgttga aggtgaacat gcagaaaggc ctgatagccc aggccagcga
    18481 tcccgcgatg gcggccgcta tgacaaatga tcagcagccg accctgctct attcactggt
    18541 gcgcaatgtc gacggcaaga ccagcgaaat agccgccaag gaagaaaccc cggtgctgcc
    18601 tggcgatgtg atcaagatca agctggcgcc gctggccagc cagtagacca acttccaaca
    18661 gatccggtgt ttcgagccag agcctcaccc gacaaaccgg gatgaggctc tggctcagtt
    18721 gtttgaacat ctcctgcgga aactataata gccgtctcgg gatcgacgct ttcccgtccg
    18781 agcacatgct tgctcaaaaa ggaaggccgc tcccttcagc ccgcgagatg ataatttatg
    18841 agggaccggc gcccgccggc tggccggcat accgaggcca gtcagtctcg cccccggccg
    18901 cgtcagccgg ctccagagcc ggtgaccgcc gcctgcgggt aacgggcagc cgcgccagct
    18961 tgaagaagct gaaatacatc aggaaagaac ccatgatgta ggggttgagt atctcgacct
    19021 cgacgaagga gcggatcagc agcaggacca tcacgccgaa aaggatcacc gagtccgcct
    19081 gccatgcctt gaagatcacc gccgagacgt ggccgtagag cgtgcgcagc atgatcagcg
    19141 acaccagcgt cgctccgacg aagccgagtt cgacgagggc ctcgatatag gtgttgtgga
    19201 agtggaagcc ggtgcgggtg gtgatgtaga attcgttcca cagccgctcg gcttcagcga
    19261 aaccctgcac ccaataggcg gcatagccga cgccgagaat cggcgatttc tggaccgtgt
    19321 tccagccttg ctcccacaga taggttcgcc cggtgagcgt cgaatccttg ccgaagaggc
    19381 cgaggacgaa gtccatcaac ccgagattca aggcgacgaa ggccgtcacg accagcaggc
    19441 cggcgccgat caggaagatg acccggcgat agcgtcgtga cagaagtttg ctcatcgcaa
    19501 gaagcgccac cagcgccaga accgcgggta tcgaggccat cgaggtggcg gaatgcgagg
    19561 cgaccaggac ataggccgac agcagcacga cgggcaccgc caggatgaag ctcagccggc
    19621 cgcgcctgta gaacgcaagg aagacgacgc agaaatagat gccgagcgag ccgacaaagc
    19681 cgatctggtt cttggaggcg aaggcgccga cgaagttgac ggtgccgtca agaacgtcct
    19741 cggaataatt cccgaccttc agcgaataga tgaggacgac gaatatcccg atcagcgccc
    19801 ctatggtcag cgtgcgcacg ctgacggtgc gcgcagctac gtaggcgcag gcaatatggg
    19861 aaaagtactg gaccgacgtc cttgcggtga cgccgggagc ctgcgaccag aaaaccgaga
    19921 agcagacata ggcagcaaac agcagcggca gccaggcgct ggacagatgc ctcacaaacc
    19981 gccggtagtc gaccaggatg agcgggagcc agaccgcata gtaggccagg atcaatatct
    20041 ggccgaagat gaccgaataa gaaaacgccc agatcgaaac ggctacggca aaggcgccat
    20101 agaccgaatt cttctcggga tcgatcagca gggactttgg aatcttcatc tcagagtccc
    20161 gccgacacga cccagggccg gcaagccatg agccggccgg aatcttcgca gacatgggcg
    20221 tgcaataaac ggcgtctcca ttgtgcagtg caatataacc tattgcgccc ggcgctcaat
    20281 ggcaaatccg gtcaccttgt cttacagcga ggcatctgcg gagagatcag ccggttgcgt
    20341 gtccgagacg ctaccaaagc gctccgccgg tgatctggat gttttccatg gtgatgccct
    20401 tggcgagatc ggagcacagg aagacggcca gagcggcaat ctcctgcggc tgcagcaggc
    20461 gctgctgcgg gttgcccctg gcgatctcgg ccatcgcttc ctctggcgtg cgccccttgc
    20521 cttcgcgctc cacgacctgc gccacattgc ggcgcatcag ttcggtctcg acccaggtcg
    20581 ggctgatcat aacgcaggtg acgccatgcg ccgccccttc cagcgccacg cagcgggtga
    20641 gacccagcag gccggccttg gacgcgcagt aggccgggtt gtccttccac ccgaccaagg
    20701 cggccgtcga gccaatgttg acgatgcggc cccagccgcg ctcgatcata cccggcagca
    20761 cggcacgcgt gacgcggaag gcgccggtca ggttggtgtc gacgatcttg tcccaaagcg
    20821 cgtcggaatg gccgcagacc ggctgctcgg cggtggtgcc ggcggcgttg accagaatgt
    20881 cggcggggcc gatggccgtc tcggcgtcag ccgcgaaacg gttggtgacg tcgctgtcgc
    20941 gaacgtcaag gtgtgcggcg tgaactttcg ttccatgagc ggcaagggcc gagcgcacgc
    21001 gctcgatctc gtcggcggcc ggatagtagg cggcatcgga tctgtcggcg ccggcaggcg
    21061 cgatgtagga gccgacggcg acattggccc cagcctcggc caaagccgtg gcaatggcga
    21121 agcccatgcc cgagaaaccg ccggtcacga tggcgctacg gccggaaagg ttgttcatgg
    21181 cgcacggtct ccgcttcgtt tgggcaggca atcagtcctg accggatttg gcaatcacag
    21241 cggcgcagcc tccatcgacg agcgttcagg acccctgaag cggtctgccc agccattcgc
    21301 gataaaagcc ttcgaggtcg ggttcgaaat catacacgat gggataaccg ggtgccgccg
    21361 cctcgacctc atgcagcgtc ccgcactccg ggcagatgaa ctcccggatt tccatccatt
    21421 cgggatcagg gagatcgctg ttgggataga tctcgcgcaa agcctcctcg gtgttgcgca
    21481 cgttaatcac ggcgctgagc ttccagttct tccgatagtc gccgaaggag tggccgcatt
    21541 cgcagcgggt aatgcgctca tcgccactct ggcagatgaa cagatggtcc gagaccggca
    21601 gcaggatcgg gtccttccag gcgacacgat cctgatagac ggcaaccatc ttgaagaaac
    21661 ggtcgtcatc cttgtaggca ctcatgatgc gccttgtctg cggccagggc agcttgccgg
    21721 cggccagatc gcccagaact tccttgctgt atgaggtcat ggcttggctc ccggcgtgaa
    21781 gatcgatttg gcgtcgacgt tccagaaatc gctgaattcc ttggtgaagc gcgacgacag
    21841 cttgatcgcg ctggcgtaca ttttcttcac ttcgggcgcg aagtcggcct ttccgacgcg
    21901 gccacgctcc ttggcgatcc aatccgccac cggaatcgcc ttagccaggc gctctttgcg
    21961 catggcggcg cggcgttcga ccgtggcgtc gatatcggcg acgggataac cgtcttcatc
    22021 gtccttcagc acaatgccga aaacctggtc cgccgcttca cgggtgagaa agccgttctc
    22081 gacatcccat gccgtcttga tcggatcgcg ctccagcacg tcgccatagc cgccaccgcc
    22141 attgtaggaa tggctgaaga tgtcgccgga tttgtgcggc gaggtgatgt aggggccttc
    22201 gaccacgaca tggtcgccgc cgatgttctt ctcgtagtcg gagacgtcag ggtcgatgcc
    22261 gggcgaatgc gccagcggtt cgccttttgc cgccagttcg tgaatgtctg agttgcgcac
    22321 cgcgcgatgc ttctggcagg tcggcgccgg ataaccaccg cacatgccgc cattgtcgaa
    22381 cacgcgcgag gagtgctcgg aggtgtggag ccgcagatgt gaggtcttgt tgatcagcca
    22441 ggtcgagacg aaggaacagc caccgcgata tttgccggcg ccgccggaat tcggcacgat
    22501 cgaacggccg atatagacca tcggcatcga ctgttcccag acctcgatgt tgcccatgtc
    22561 ggattccggg ttccagccga cataggcggt gtcgagaccg tccttgatgg cgagcgcccc
    22621 ggacccggcg gcagcgcact cgaaatgcgc catgccgaac ggcgtgccgt attggctttc
    22681 gccgcccatc tcgatcatcg ggctgttgac ctggccgaca aaggcctcct cgacgaagcc
    22741 gcgcgcgaca aaactgcgcg acaaaagccg ctggaaaacg c
//
