LOCUS       NZ_BBVA01000024         3707 bp    DNA     linear   CON 10-SEP-2024
DEFINITION  Micromonospora sp. NBRC 110037, whole genome shotgun sequence.
ACCESSION   NZ_BBVA01000024
VERSION     NZ_BBVA01000024.1
KEYWORDS    WGS; HIGH_QUALITY_DRAFT; RefSeq.
SOURCE      Micromonospora sp. NBRC 110037
  ORGANISM  Micromonospora sp. NBRC 110037
            Bacteria; Actinomycetota; Actinomycetes; Micromonosporales;
            Micromonosporaceae; Micromonospora.
REFERENCE   1
  AUTHORS   Igarashi,Y., Komaki,H., Ichikawa,N., Hosoyama,A. and Fujita,N.
  TITLE     Whole genome shotgun sequence of Micromonospora sp. NBRC 110037
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3707)
  AUTHORS   Hosoyama,A., Ichikawa,N., Kimura,A., Ohshita,R. and Fujita,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2015) Contact:Director-General Department Of
            Biotechnology National Institute of Technology and Evaluation,
            Biological Resource Center (NBRC); 2-49-10 Nishihara, Shibuya-ku,
            Tokyo 151-0066, Japan URL :http://www.nite.go.jp/nbrc/index.html
COMMENT     ##Genome-Assembly-Data-START##
            Finishing Goal                    :: Noncontiguous Finished
            Current Finishing Status          :: High-Quality Draft
            Assembly Method                   :: newbler v. 2.6
            Genome Coverage                   :: 106x
            Sequencing Technology :: 454 GS-FLX Titanium; Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_001652995.1-RS_2024_09_10
            Annotation Date                   :: 09/10/2024 02:06:42
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,195
            CDSs (total)                      :: 6,136
            Genes (coding)                    :: 6,073
            CDSs (with protein)               :: 6,073
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 15 of 63
            Pseudo Genes (incomplete)         :: 53 of 63
            Pseudo Genes (internal stop)      :: 4 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 11:59:58
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 0
            Orig. end                         :: 3707
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            BBVA01000024.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     gene            complement(<1..>3653)
                     /locus_tag="A4R25_RS30380"
                     /pseudo=""
     CDS             complement(<1..3653)
                     /NRPS_PKS="Domain: ACP (56-128). E-value: 1.1e-32. Score:
                     104.2. Matches aSDomain:
                     nrpspksdomains_A4R25_RS30380_ACP.1"
                     /NRPS_PKS="Domain: PKS_KS(Modular-KS) (149-568). E-value:
                     6.9e-178. Score: 583.8. Matches aSDomain:
                     nrpspksdomains_A4R25_RS30380_PKS_KS.1"
                     /NRPS_PKS="Domain: PKS_AT (665-963). E-value: 1.3e-109.
                     Score: 358.1. Matches aSDomain:
                     nrpspksdomains_A4R25_RS30380_PKS_AT.1"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters) T1PKS:
                     mod_KS"
                     /gene_functions="biosynthetic (rule-based-clusters) T1PKS:
                     PKS_AT"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PP-binding"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) ketoacyl-synt"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1021: malonyl CoA-acyl carrier protein transacylase"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013285630.1"
                     /locus_tag="A4R25_RS30380"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /pseudo=""
                     /sec_met_domain="PP-binding (E-value: 1.2e-17, bitscore:
                     50.5, seeds: 164, tool: rule-based-clusters)"
                     /sec_met_domain="mod_KS (E-value: 4.5e-217, bitscore:
                     707.0, seeds: 217, tool: rule-based-clusters)"
                     /sec_met_domain="ketoacyl-synt (E-value: 4.9e-91, bitscore:
                     290.8, seeds: 77, tool: rule-based-clusters)"
                     /sec_met_domain="PKS_AT (E-value: 2.1e-111, bitscore:
                     358.1, seeds: 1682, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MPSFTALRPSRLFTDIPEAQAAIEAASRAETGPTDRASALRERLA
                     ALTPPEVEASLLDLVRSQVATVLGHATVEAVRPDRAFQRLGFDSLTAVELRNRLTAETG
                     LALPSTLVFDHPTPAALVAFIRETVLGASGDAPADRRVATVDDDPIVIVGMGCRLPGGV
                     ASPEELWQLLATGRDGISEFPLDRGWDSFLSSGLSDTSFARRGGFVYDAGEFDAEFFGI
                     SPREALAMDPQQRLLLETSWEAVESAGIDPTTLRGGAVGVFAGASFQGYASGSVGRAQE
                     VGGHLLTGNATSVLSGRVAYSFGFEGPAVTVDTACSSSLVALHLAAQALRSGECDLALA
                     GGVTVMASPGTFAEFSIQGGLSADGRCKSFSDAADGTGWSEGVGVLVVQRLSDARRDGR
                     RVLAVVRGSAVNQDGASNGLTAPNGPSQQRVIRQALASAGLSAADVDVVEAHGTGTSLG
                     DPIEAQALLATYGQGRDVPLLLGSVKSNIGHTQAAAGVAGIIKMILAMRYGTVPATLHV
                     DVPSSHVDWSSGAVELVTRAREWPGAGRARRAAVSSFGISGTNAHVIIEQAEPEHAAAS
                     AVPVGVPVPWVLSGRSERGLSGQAGRLGSFVRGRGLSPVDVSWSLATTRAALEYRAVVW
                     GSDVDELVAGLAAVAEGRSAGVVSAGRRAVLFTGQGSQRAGMGRELYEAFPVFAASFDE
                     VCAEIDPRLPRALREVVFAEPGSETAALVDQTVFAQAGLFAVEVALWELLASWGVRADF
                     LAGHSIGEVTAAYVSGMLSLRDACTLVAARGRLMQALPAGGVMAAVGASEEAVAELLGA
                     SGAVVDIAAVNGPASVVVSGAADEVASVVGACCERGWRVKELAVSHAFHSRLMDPMLDE
                     FASVVAGLDWQPPRIPIVSNVTGAVADAAEITEPGYWVRHVRQPVRFADGVAALRAQGV
                     DTFLEIGPDAVLTAMAAEADTADDVRYVATLRRSQPDVTTLTTAAGQLWAAGVAVDWAA
                     YLGQTGTRPRAVELPTYAFDRQRYWLEDPQPGSAPERADAPSDEQFWAAVESGDLGVLG
                     EDLAVGADEPSTALLPKLARWRRATQQRAVVDSWRYRATWRTAAVPDSATLAGTWLLLM
                     APGQEDHPVAAALAARADRAVPVLIPAGADRDRVARLLLEAMSSDARDAHVVSLLSLAE
                     PREASPVPAAAEVSTALAVVQALTDVGGSGRLWWLTRGAVSVGDSDELADV"
     source          1..3707
                     /culture_collection="NBRC:110037"
                     /db_xref="taxon:1621261"
                     /mol_type="genomic DNA"
                     /note="contig: scaffold00026"
                     /organism="Micromonospora sp. NBRC 110037"
                     /strain="NBRC 110037"
     region          1..3707
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="T1PKS"
                     /region_number="1"
                     /rules="cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or
                     hyb_KS or itr_KS or tra_KS))"
                     /tool="antismash"
     cand_cluster    1..3707
                     /SMILES=""
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="cds(PKS_AT and (PKS_KS or ene_KS or
                     mod_KS or hyb_KS or itr_KS or tra_KS))"
                     /kind="single"
                     /product="T1PKS"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..3707
                     /aStool="rule-based-clusters"
                     /category="PKS"
                     /contig_edge="True"
                     /core_location="[<0:3653](-)"
                     /cutoff="20000"
                     /detection_rule="cds(PKS_AT and (PKS_KS or ene_KS or mod_KS
                     or hyb_KS or itr_KS or tra_KS))"
                     /neighbourhood="20000"
                     /product="T1PKS"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(<1..3653)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="cds(PKS_AT and (PKS_KS or ene_KS or mod_KS
                     or hyb_KS or itr_KS or tra_KS))"
                     /neighbourhood="20000"
                     /product="T1PKS"
                     /protocluster_number="1"
     aSDomain        complement(765..1658)
                     /ASF="Malonyl-CoA specific"
                     /ASF="found active site serine: True, scaffold matched
                     GHGE: True"
                     /aSDomain="PKS_AT"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_A4R25_RS30380_PKS_AT.1"
                     /evalue="1.30E-109"
                     /label="A4R25_RS30380_PKS_AT.1"
                     /locus_tag="A4R25_RS30380"
                     /protein_end="963"
                     /protein_start="665"
                     /score="358.1"
                     /specificity="substrate consensus: mal"
                     /specificity="PKS signature: Malonyl-CoA"
                     /specificity="Minowa: mal"
                     /tool="antismash"
                     /translation="LFTGQGSQRAGMGRELYEAFPVFAASFDEVCAEIDPRLPRALREV
                     VFAEPGSETAALVDQTVFAQAGLFAVEVALWELLASWGVRADFLAGHSIGEVTAAYVSG
                     MLSLRDACTLVAARGRLMQALPAGGVMAAVGASEEAVAELLGASGAVVDIAAVNGPASV
                     VVSGAADEVASVVGACCERGWRVKELAVSHAFHSRLMDPMLDEFASVVAGLDWQPPRIP
                     IVSNVTGAVADAAEITEPGYWVRHVRQPVRFADGVAALRAQGVDTFLEIGPDAVLTAMA
                     AEADTADDVRYVATLRR"
     aSModule        complement(765..3206)
                     /domains="nrpspksdomains_A4R25_RS30380_PKS_KS.1"
                     /domains="nrpspksdomains_A4R25_RS30380_PKS_AT.1"
                     /incomplete
                     /locus_tags="A4R25_RS30380"
                     /tool="antismash"
                     /type="pks"
     CDS_motif       complement(900..935)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_A4R25_RS30380_0009"
                     /evalue="2.30E-05"
                     /label="PKSI-AT-M_m8"
                     /locus_tag="A4R25_RS30380"
                     /protein_end="918"
                     /protein_start="906"
                     /score="17.6"
                     /tool="antismash"
                     /translation="YWVRHVRQPVRF"
     CDS_motif       complement(972..1004)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_A4R25_RS30380_0008"
                     /evalue="4.90E-03"
                     /label="PKSI-AT-M_m7"
                     /locus_tag="A4R25_RS30380"
                     /protein_end="894"
                     /protein_start="883"
                     /score="9.7"
                     /tool="antismash"
                     /translation="PRIPIVSNVTG"
     CDS_motif       complement(1158..1196)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_A4R25_RS30380_0007"
                     /evalue="5.60E-04"
                     /label="PKSI-AT-M_m5"
                     /locus_tag="A4R25_RS30380"
                     /protein_end="832"
                     /protein_start="819"
                     /score="13.2"
                     /tool="antismash"
                     /translation="AAVNGPASVVVSG"
     CDS_motif       complement(1290..1412)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_A4R25_RS30380_0006"
                     /evalue="8.00E-18"
                     /label="PKSI-AT-M_m3"
                     /locus_tag="A4R25_RS30380"
                     /protein_end="788"
                     /protein_start="747"
                     /score="56.6"
                     /tool="antismash"
                     /translation="GVRADFLAGHSIGEVTAAYVSGMLSLRDACTLVAARGRLMQ"
     CDS_motif       complement(1608..1655)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_A4R25_RS30380_0005"
                     /evalue="2.60E-06"
                     /label="PKSI-AT-M_m1"
                     /locus_tag="A4R25_RS30380"
                     /protein_end="682"
                     /protein_start="666"
                     /score="19.8"
                     /tool="antismash"
                     /translation="FTGQGSQRAGMGRELY"
     aSDomain        complement(1950..3206)
                     /ASF="found active site cysteine: True, scaffold matched
                     GSSS: True"
                     /ASF="found active site histidines: True"
                     /aSDomain="PKS_KS"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_A4R25_RS30380_PKS_KS.1"
                     /domain_subtypes="Modular-KS"
                     /evalue="6.90E-178"
                     /label="A4R25_RS30380_PKS_KS.1"
                     /locus_tag="A4R25_RS30380"
                     /protein_end="568"
                     /protein_start="149"
                     /score="583.8"
                     /tool="antismash"
                     /translation="IVIVGMGCRLPGGVASPEELWQLLATGRDGISEFPLDRGWDSFLS
                     SGLSDTSFARRGGFVYDAGEFDAEFFGISPREALAMDPQQRLLLETSWEAVESAGIDPT
                     TLRGGAVGVFAGASFQGYASGSVGRAQEVGGHLLTGNATSVLSGRVAYSFGFEGPAVTV
                     DTACSSSLVALHLAAQALRSGECDLALAGGVTVMASPGTFAEFSIQGGLSADGRCKSFS
                     DAADGTGWSEGVGVLVVQRLSDARRDGRRVLAVVRGSAVNQDGASNGLTAPNGPSQQRV
                     IRQALASAGLSAADVDVVEAHGTGTSLGDPIEAQALLATYGQGRDVPLLLGSVKSNIGH
                     TQAAAGVAGIIKMILAMRYGTVPATLHVDVPSSHVDWSSGAVELVTRAREWPGAGRARR
                     AAVSSFGISGTNAHVIIEQA"
     CDS_motif       complement(2169..2213)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_A4R25_RS30380_0004"
                     /evalue="4.20E-04"
                     /label="PKSI-KS_m6"
                     /locus_tag="A4R25_RS30380"
                     /protein_end="495"
                     /protein_start="480"
                     /score="12.6"
                     /tool="antismash"
                     /translation="GSVKSNIGHTQAAAG"
     CDS_motif       complement(2268..2312)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_A4R25_RS30380_0003"
                     /evalue="2.00E-04"
                     /label="PKSI-KS_m5"
                     /locus_tag="A4R25_RS30380"
                     /protein_end="462"
                     /protein_start="447"
                     /score="13.4"
                     /tool="antismash"
                     /translation="VEAHGTGTSLGDPIE"
     CDS_motif       complement(2688..2735)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_A4R25_RS30380_0002"
                     /evalue="5.60E-07"
                     /label="PKSI-KS_m3"
                     /locus_tag="A4R25_RS30380"
                     /protein_end="322"
                     /protein_start="306"
                     /score="21.3"
                     /tool="antismash"
                     /translation="GPAVTVDTACSSSLVA"
     CDS_motif       complement(2937..2963)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_A4R25_RS30380_0001"
                     /evalue="1.80E-02"
                     /label="PKSI-KS_m2"
                     /locus_tag="A4R25_RS30380"
                     /protein_end="239"
                     /protein_start="230"
                     /score="8.1"
                     /tool="antismash"
                     /translation="DPQQRLLLE"
     aSDomain        complement(3270..3485)
                     /ASF="found active site serine: True"
                     /ASF="non-beta-branching ACP"
                     /aSDomain="ACP"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_A4R25_RS30380_ACP.1"
                     /evalue="1.10E-32"
                     /label="A4R25_RS30380_ACP.1"
                     /locus_tag="A4R25_RS30380"
                     /protein_end="128"
                     /protein_start="56"
                     /score="104.2"
                     /tool="antismash"
                     /translation="LDLVRSQVATVLGHATVEAVRPDRAFQRLGFDSLTAVELRNRLTA
                     ETGLALPSTLVFDHPTPAALVAFIRET"
     misc_feature    complement(3639..3654)
                     /note="TFBS match to CRP, Cyclic AMP receptor protein,
                     confidence: weak, score: 15.8"
                     /tool="antismash"
ORIGIN
        1 gcgacatccg ccagctcgtc cgagtcgccg accgacaccg ccccgcgcgt cagccaccac
       61 aaccggcccg acccaccgac gtcggtcagc gcctgcacca cggccagcgc cgtcgacacc
      121 tcggcggcgg ccggaaccgg ggacgcctcc cgcggctcgg ccagcgacag cagggacacc
      181 acgtgggcgt cccgggcgtc ggagctcatc gcctccagca gcagccgcgc cacccggtcg
      241 cggtcggcgc cggccgggat cagcaccggg accgcccggt cggcccgggc agccagcgcc
      301 gcggccaccg ggtggtcctc ctgcccgggc gccatcagca gcagccaggt tcccgccaac
      361 gtcgcggagt ccggcacggc ggccgtacgc cacgtcgccc ggtaccgcca ggagtcgacg
      421 accgcccgct gctgcgtcgc acgccgccac cgggcgagct tgggcagcag cgccgtggac
      481 ggctcgtccg cgcccacggc gagatcctcg cccagcacgc cgaggtcccc ggactcgacc
      541 gccgcccaga actgctcgtc ggacggggcg tcggcccgct ccggagccga gccgggctgg
      601 gggtcctcca gccagtagcg ctgcctgtcg aaggcgtagg tgggcagttc caccgcgcgc
      661 ggccgggtgc cggtctggcc gaggtacgcc gcccagtcga cggccacgcc ggcggcccag
      721 agctgcccgg cggcagtggt gagggtggtg acgtcgggct ggctgcggcg cagcgtggcg
      781 acgtaccgca cgtcgtcggc ggtgtccgcc tcggcggcca tggcggtgag gaccgcgtcc
      841 gggccgatct ccaggaaggt gtccaccccc tgtgcccgca gagcagccac cccgtcggcg
      901 aagcgcaccg gctgccgcac gtgccgcacc cagtagccgg gctcggtgat ctctgcggcg
      961 tccgcgaccg cgccggtcac gttcgacacg attgggatcc gcggcggttg ccagtcgagc
     1021 cccgccacca ccgacgcgaa ctcgtccagc atcggatcca tcaaccgcga atggaaggcg
     1081 tgactgaccg ccagttcctt gacccgccaa ccccgctcac agcaggcccc caccaccgaa
     1141 gccacctcat cggccgcgcc cgagacgacc accgacgcgg ggccgttcac cgccgcaatg
     1201 tccacgaccg cgcccgacgc gccgagcagc tcggcgacag cctcctccga cgcaccgacc
     1261 gccgccatca cgccaccggc cggcaacgcc tgcatcaacc ggccccgcgc cgccaccaac
     1321 gtgcacgcgt ccctcaggga caacatcccc gacacgtacg ccgccgtcac ctcaccaatc
     1381 gaatgacccg ccaggaaatc agcccgcacc ccccacgacg cgagcaactc ccacaacgcc
     1441 acctcaacag cgaacaaccc cgcctgcgcg aacacggtct gatccaccag agccgcagtc
     1501 tccgaacccg gctccgcgaa caccacctcg cgcaacgcac gcggcagacg cggatcaatc
     1561 tccgcgcaca cttcgtcgaa cgacgccgcg aacaccggga acgcctcgta cagctcccgg
     1621 cccatacccg cacgctgcga accctgaccc gtgaacaaca ccgcccgacg acccgccgac
     1681 accacacccg cagaccgacc ctcagccacc gccgcaaggc ccgccaccag ctcgtccacg
     1741 tccgagcccc acaccaccgc ccgatactcc aacgccgccc gagtcgtcgc caacgaccac
     1801 gacacatcca ccggcgacag accacgacca cgcacgaacg accccagccg ccccgcctga
     1861 cccgacagac cccgctccga acgccccgac agcacccacg gcaccggcac cccgacgggc
     1921 acggcagacg ccgcggcgtg ctccggctcc gcctgctcga tgatcacgtg cgcgttcgtg
     1981 ccggagatcc cgaacgacga cacggcagcc ctgcgtgcgc ggcccgcacc cggccactcg
     2041 cgcgcccggg tcaccagctc caccgcgccc gacgaccaat ccacatgcga cgacggcaca
     2101 tccacatgca gcgtcgccgg cacggtcccg taccgcatcg ccaagatcat cttgatgatg
     2161 cccgccaccc ccgcagccgc ctgcgtatga ccgatgttcg acttcaccga gccgagcaac
     2221 agcggcacat cccggccctg cccgtacgtc gccagcaacg cctgcgcctc gatcggatca
     2281 cccaacgacg tacccgtacc gtgcgcctcc accacatcca catcagccgc cgacaaaccc
     2341 gcactcgcca gcgcctgccg gatcacccgc tgctgcgacg gaccattcgg cgccgtcaaa
     2401 ccattcgacg caccatcctg attcaccgcc gaaccccgca ccaccgccag cacccgacgc
     2461 ccgtcacgcc gcgcatccga caagcgctgc accaccagca cgccgacgcc ctcggaccag
     2521 cccgtgccgt ccgcggcgtc cgagaacgac ttgcagcggc cgtcggcgga caggccgccc
     2581 tggatcgaga actcggcgaa cgtgcccggt gaggccatca cggtgacgcc gccggccagg
     2641 gcgaggtcac actcgccgga ccgcagcgcc tgcgccgcca ggtgcaacgc cacaagcgac
     2701 gacgaacacg ccgtgtccac agtcaccgcc ggcccctcga aaccgaacga gtacgccacc
     2761 cggcccgaca acacgcttgt cgcgttgccg gtgagcaggt ggccgccgac ctcctgggcc
     2821 cgaccgaccg acccgctggc gtacccctgg aagctggccc cggcgaagac gccgaccgcg
     2881 ccgccccgca gggtggtcgg gtcgatgccc gccgactcga ctgcctccca cgaggtctcc
     2941 agcagcagcc gctgctgcgg gtccatggcg agcgcctccc gcggcgagat gccgaagaac
     3001 tcggcgtcga actcgccggc gtcgtagacg aagccgcccc tgcgggcgaa ggacgtgtcg
     3061 gacaggccgc tgctgaggaa gctgtcccag ccccggtcga gggggaactc ggagatgccg
     3121 tcgcgtccgg tggcgagcag ttgccacagt tcttccgggg acgcgacgcc acccgggagg
     3181 cggcagccca tgccgacgat cacgatcggg tcgtcgtcga cggtggcgac cctgcggtcg
     3241 gccggcgcgt cgcccgatgc tccgagcacg gtctcccgga tgaaggcgac cagggcggcc
     3301 ggcgtcgggt ggtcgaagac gagggtggag ggcaacgcga ggccggtttc ggcggtcagc
     3361 cggttgcgca gctcgactgc ggtgagcgag tcgaagccga ggcgctggaa cgcccggtcc
     3421 ggccgcaccg cctccacggt ggcgtggccg agcaccgtgg cgacctggga gcgtacgagg
     3481 tccaggaggg acgcctcgac ctccggcggc gtcagggccg cgagccgctc gcgcagtgcg
     3541 gaggcccggt cggtggggcc ggtctcggct cgggacgccg cctcgatcgc cgcctgtgct
     3601 tcggggatgt cggtgaacaa ccgggacgga cgcaacgccg tgaacgacgg cacaaaccga
     3661 tcccaccgca catccgccac caccacctgc ccctcaccac catccaa
//
