LOCUS       NZ_BBVA01000006        14813 bp    DNA     linear   CON 10-SEP-2024
DEFINITION  Micromonospora sp. NBRC 110037, whole genome shotgun sequence.
ACCESSION   NZ_BBVA01000006
VERSION     NZ_BBVA01000006.1
KEYWORDS    WGS; HIGH_QUALITY_DRAFT; RefSeq.
SOURCE      Micromonospora sp. NBRC 110037
  ORGANISM  Micromonospora sp. NBRC 110037
            Bacteria; Actinomycetota; Actinomycetes; Micromonosporales;
            Micromonosporaceae; Micromonospora.
REFERENCE   1
  AUTHORS   Igarashi,Y., Komaki,H., Ichikawa,N., Hosoyama,A. and Fujita,N.
  TITLE     Whole genome shotgun sequence of Micromonospora sp. NBRC 110037
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 441257)
  AUTHORS   Hosoyama,A., Ichikawa,N., Kimura,A., Ohshita,R. and Fujita,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAR-2015) Contact:Director-General Department Of
            Biotechnology National Institute of Technology and Evaluation,
            Biological Resource Center (NBRC); 2-49-10 Nishihara, Shibuya-ku,
            Tokyo 151-0066, Japan URL :http://www.nite.go.jp/nbrc/index.html
COMMENT     ##Genome-Assembly-Data-START##
            Finishing Goal                    :: Noncontiguous Finished
            Current Finishing Status          :: High-Quality Draft
            Assembly Method                   :: newbler v. 2.6
            Genome Coverage                   :: 106x
            Sequencing Technology :: 454 GS-FLX Titanium; Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_001652995.1-RS_2024_09_10
            Annotation Date                   :: 09/10/2024 02:06:42
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,195
            CDSs (total)                      :: 6,136
            Genes (coding)                    :: 6,073
            CDSs (with protein)               :: 6,073
            Genes (RNA)                       :: 59
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 51
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 63
            CDSs (without protein)            :: 63
            Pseudo Genes (ambiguous residues) :: 0 of 63
            Pseudo Genes (frameshifted)       :: 15 of 63
            Pseudo Genes (incomplete)         :: 53 of 63
            Pseudo Genes (internal stop)      :: 4 of 63
            Pseudo Genes (multiple problems)  :: 9 of 63
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 11:59:58
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 293848
            Orig. end                         :: 308661
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            BBVA01000006.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..14813
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="NAGGN"
                     /region_number="1"
                     /rules="((TIGR03104 or cds(GATase_7 and Asn_synthase)) and
                     TIGR03103 and (TIGR03106 or Peptidase_M42))"
                     /tool="antismash"
     cand_cluster    1..14813
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="((TIGR03104 or cds(GATase_7 and
                     Asn_synthase)) and TIGR03103 and (TIGR03106 or
                     Peptidase_M42))"
                     /kind="single"
                     /product="NAGGN"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..14813
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:9813](-)"
                     /cutoff="10000"
                     /detection_rule="((TIGR03104 or cds(GATase_7 and
                     Asn_synthase)) and TIGR03103 and (TIGR03106 or
                     Peptidase_M42))"
                     /neighbourhood="5000"
                     /product="NAGGN"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..9813)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="10000"
                     /detection_rule="((TIGR03104 or cds(GATase_7 and
                     Asn_synthase)) and TIGR03103 and (TIGR03106 or
                     Peptidase_M42))"
                     /neighbourhood="5000"
                     /product="NAGGN"
                     /protocluster_number="1"
     misc_feature    complement(124..138)
                     /note="TFBS match to VqsM, Regulator of quorum-sensing
                     signalling systems, confidence: medium, score: 17.8"
                     /tool="antismash"
     gene            complement(175..1221)
                     /locus_tag="A4R25_RS19810"
     CDS             complement(175..1221)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018787506.1"
                     /locus_tag="A4R25_RS19810"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="WP_043326540.1"
                     /transl_table=11
                     /translation="MGDAGSVAPVHWQRVRQIARLAAIGTGATVAATAATGGVLLGQAR
                     QARRTIPMAQAPPPRCDGVYGAKLPGPPVTMVILGDSSAAGYGVHRRRETPGSLLATGL
                     SRRLHRPVRLHRFAVVGALSSGLKPQVESALEVEPDVAVILVGGNDVTNRTPPGLAVRY
                     LVDAVRTLRAAGCEVVVGTCPDLGTIRPIQPPLRWLARRWSRQLAAAQTVGVVEAGGRA
                     VSLGDMLGPRFAAEPARMFAWDRFHPSAEGYAIAAAALLPTVLSALGAVPERRPVLPGV
                     EGVRSLPEAAHEAARHAGTEVSGAQLRGRDRGPGGRWAQLRRRAFFGVGALPPTGSAAD
                     SATLEETA"
     gene            1363..1719
                     /locus_tag="A4R25_RS19815"
     CDS             1363..1719
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007074683.1"
                     /locus_tag="A4R25_RS19815"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="YkvA family protein"
                     /protein_id="WP_013474058.1"
                     /transl_table=11
                     /translation="MGKTLKRSAAFAALARALAAGTRGGPSLGARLAALPRMIRATTRG
                     EYDGGLRLALMAGATAYIVSPIDLLPEIPLAIFGLADDAVMVTWLAGSVLAETERFLEW
                     EARRSSVIPGHVVP"
     gene            1796..3166
                     /locus_tag="A4R25_RS19820"
     CDS             1796..3166
                     /EC_number="4.2.1.22"
                     /GO_component="GO:0005737 - cytoplasm [Evidence IEA]"
                     /GO_function="GO:0004122 - cystathionine beta-synthase
                     activity [Evidence IEA]"
                     /GO_process="GO:0019343 - cysteine biosynthetic process via
                     cystathionine [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PALP"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1081: cysteine synthase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018742048.1"
                     /locus_tag="A4R25_RS19820"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="cystathionine beta-synthase"
                     /protein_id="WP_043326537.1"
                     /sec_met_domain="PALP (E-value: 3.6e-73, bitscore: 241.2,
                     seeds: 145, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MQYYDNVVELIGNTPLVRLRNVTEGIQATVLAKVEYVNPGGSVKD
                     RIAMRMVEDAEKAGILRPGGTIVEPTSGNTGVGLALVAQLKGYRCVFVCPDKVSQDKQD
                     VLRAYGAEVVVCPTAVAPEDPRSYYNVSDRLAREIPGAWKPNQYSNPANPRSHYETTGP
                     ELWKQTEGRITHFVAGVGTGGTISGIGRYLKEASEGRVKVIGADPEGSVYSGGTGRPYL
                     VEGVGEDFWPETYDRKIADEIVEVSDKESFEMTRRLAREEGLLVGGSCGMAVVGALEVA
                     RKAGPDDVIVVLLPDGGRGYLSKIFNDKWMARYGFLDDSGTEPTVADALAGKPGGLPEL
                     VHVHPTETVRDAIDYMREYGVSQLPVLKAEPPVVTGEVAGSIAEKDLLDALFTGQAHLH
                     DTIERHMGEPLPMIGGGQPVSEAVGLLEKADAALVLVDGKPKGVLTRQDLLAHLGAR"
     gene            complement(3256..3765)
                     /locus_tag="A4R25_RS19825"
     CDS             complement(3256..3765)
                     /EC_number="2.3.-.-"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018742049.1"
                     /locus_tag="A4R25_RS19825"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="WP_043326535.1"
                     /transl_table=11
                     /translation="MTISLRSATEADLMAVGALHQRSRVAAYSSFLPPEALADPTPEAM
                     GRYWVERLTWEGADHRMTVAERDGKLVGFSYLGPDDEGDPATGLLNAIHLEPSERGRGT
                     GRALMVDALDAMRARGWTRAVLWVLRDNAHARRFYERGGWTATGDERDEHIGRVLVPQL
                     RYARRL"
     gene            complement(3762..4691)
                     /locus_tag="A4R25_RS19830"
     CDS             complement(3762..4691)
                     /EC_number="3.1.26.11"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018586560.1"
                     /locus_tag="A4R25_RS19830"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="ribonuclease Z"
                     /protein_id="WP_043326533.1"
                     /transl_table=11
                     /translation="MSMRELVVLGTASQAPTRQRNHNGYLLRWDDEVILFDPGEGSQRQ
                     MLHTGVTATDLTRICVTHFHGDHCLGLPGMIQRLSLDRVPRPVAVHFPAGGAEYFARLR
                     HASSFYETAELAVEPIDVDGQRITLGVGTLEARRLRHPIETYGYRLVEPDGCRMLPERL
                     AAYGIAGPDVGRLLRDGHLDRDGRRVTRDEVSVVRPGQRFAFVMDTGLCDGVYALAEHA
                     DLLVIESTFLESEAALAAEVGHLTAGQAARVAAESGVRTLVLTHFSQRYADPRRFHDEA
                     REHFSGDLVIAEDLQTVQVPPRRVPSPG"
     misc_feature    4914..4929
                     /note="TFBS match to DasR, N-acetylglucosamine dependent
                     repressor, confidence: weak, score: 20.95"
                     /tool="antismash"
     gene            complement(5001..6185)
                     /locus_tag="A4R25_RS19835"
     CDS             complement(5001..6185)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters) NAGGN:
                     Peptidase_M42"
                     /gene_functions="biosynthetic (rule-based-clusters) NAGGN:
                     TIGR03106"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018787511.1"
                     /locus_tag="A4R25_RS19835"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="osmoprotectant NAGGN system M42 family peptidase"
                     /protein_id="WP_030501323.1"
                     /sec_met_domain="TIGR03106 (E-value: 2.2e-147, bitscore:
                     485.0, seeds: 7, tool: rule-based-clusters)"
                     /sec_met_domain="Peptidase_M42 (E-value: 2e-57, bitscore:
                     189.1, seeds: 48, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTARKPQPLKLDLDYLRQVLVELLEIPSPSGRTDHIQQYVGERLS
                     AIGISSTLTRRGALSACLPGPRSTGADRAIVVHTDTIGGMVKRLKENGRLEVNPIGTHS
                     ARFAEGAHVRIFTDDLERVVTGQVLPLKASGHRYNEAVDLQGVGWELVEVRVDEPVHDE
                     AGLRALGIDAGDFVAFLPNPTITPNGYVKSRHLDDKAGVAAVLTAFKAMVDAGVTPAVT
                     AHLLVTVTEEIGHGASHGLDPDVAEIVSVDAAVVAPGQQSRETAATLAMGDGVGPFDYH
                     LTRNLASIAKEHGVDLVRDVFDYYRSDVAAAVEAGAHARVALLGFGVDATHGHERTHLD
                     GLHQLTQLLCLYLQSPLVFPEWDAEPEGDLADFPSLAVQPANEDGPREGPIGLD"
     gene            complement(6182..8020)
                     /gene="ngg"
                     /locus_tag="A4R25_RS19840"
     CDS             complement(6182..8020)
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /GO_function="GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /gene="ngg"
                     /gene_functions="biosynthetic (rule-based-clusters) NAGGN:
                     TIGR03103"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) RimK"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Dala_Dala_lig_C"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018810760.1"
                     /locus_tag="A4R25_RS19840"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="N-acetylglutaminylglutamine synthetase"
                     /protein_id="WP_043326531.1"
                     /sec_met_domain="TIGR03103 (E-value: 6.7e-240, bitscore:
                     791.8, seeds: 7, tool: rule-based-clusters)"
                     /sec_met_domain="RimK (E-value: 1.6e-08, bitscore: 29.1,
                     seeds: 4, tool: rule-based-clusters)"
                     /sec_met_domain="Dala_Dala_lig_C (E-value: 3.8e-08,
                     bitscore: 27.7, seeds: 12, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTDTLATGTARTDRERVLGRRRERIGPGGDPVAPGTPEQRHPEPT
                     STDASGVVLDCGWGRLVFGQTFDDQVHVADVLRSEAVGARDICIYLRDPHVLVSRLPDE
                     LFIDPSLTYRLPLGEKRPAATEGGEVPGLTVRPLRDAVDAEAVNRIYARNGMVTAPVEV
                     LVDNAGTDRFLHLVAEDTTGEVVGTITGVDHVAVFGDPENGASLWCLTVDFNTAPPGTG
                     QALLTALADRLDGRGRAFVDLSVLAENSGAIRLYERLGFHRTGTLCVKRKNPINERLFL
                     PAMPEGYDELNPYAKIVADEAMRRGIRVEVTDPHWGELKLTTGGRTIHTRESLSELTSA
                     VAMSRCDDKRVTRRILTEAGLSVPRGRTATGEPDDVAFLNDVGPVVVKPARGEQGNGIT
                     VGVRTPEALTAAVELARRFCPDVLIEELRAGEDLRVVVIDHEVVAAAVRRPAQITGDGV
                     HDITELIERQSRRRAAATGGESRIPIDDMTREVVAEAGYRMHDVLPEGEVLAVRRTANL
                     HTGGTIHDVTAELHPAIAEACVTASRALDIPVTGLDLLVPAPDRPEHVFIEANERPGLA
                     NHEPQPTAERFVDLLFPGTRAPQRLWSPAGAASSGA"
     gene            complement(8023..9813)
                     /locus_tag="A4R25_RS19845"
     CDS             complement(8023..9813)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters) NAGGN:
                     TIGR03104"
                     /gene_functions="biosynthetic (rule-based-clusters) NAGGN:
                     Asn_synthase"
                     /gene_functions="biosynthetic (rule-based-clusters) NAGGN:
                     GATase_7"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1177: asparagine synthase (glutamine-hydrolyzing)"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007074689.1"
                     /locus_tag="A4R25_RS19845"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="N-acetylglutaminylglutamine amidotransferase"
                     /protein_id="WP_064447691.1"
                     /sec_met_domain="TIGR03104 (E-value: 3.5e-286, bitscore:
                     944.7, seeds: 10, tool: rule-based-clusters)"
                     /sec_met_domain="GATase_7 (E-value: 1.3e-41, bitscore:
                     136.1, seeds: 307, tool: rule-based-clusters)"
                     /sec_met_domain="Asn_synthase (E-value: 2.1e-87, bitscore:
                     288.7, seeds: 78, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MCGLAGEFRRDGSRADVSAVERMAATMSDRGPDDSGVWSQGPTAL
                     GHRRLKIIDLSAASGQPIVDAAAGLTGVFNGCIYNYRELRAELQAKGHRFFSSGDSEVV
                     VKAYAEWGLDFVDHLIGMFAVAISERDTGRLVLARDRLGIKPLYLAESPGVVRFASTLP
                     ALLAGGGIDTTIDRVALAHYLSFHSIVPPPRTILAGVTKLPPATVRVYEADGSTHERVY
                     WDPSFGRAAERAGWSERDWQDALLDSLTTAVRRRMVADVPVGVLLSGGLDSSLVVALLA
                     GEGQARSGLATFSIGFDAVGGREGDEFVYSDLVAKTFGTDHHQIRVPTGDLLPPLESAV
                     GAMSEPMVSHDCVAFWLLSQEVAKHVKVVQSGQGADEILGGYHWYPPLAGVDREQALET
                     YAKAFFDRDAAGLARVLNPAWLADGDPAREFVAAHLGRPGAQTAVDAGLRIDTQVMLTD
                     DPVKRVDNMTMAHGLEARVPFLDHEFVELAASCPPELKLAQGGKGVLKEIGRRVLPHEV
                     IDRPKGYFPVPGLTHLEGKLLDRVRDALTAPEARRRDLFRADYVDALLADPNAELTPLN
                     GNKLWQLGLLEMWLQSHGID"
     misc_feature    complement(9844..9858)
                     /note="TFBS match to SypG, Regulator of biofilm formation
                     and host colonization, confidence: weak, score: 17.52"
                     /tool="antismash"
     misc_feature    9894..9907
                     /note="TFBS match to BldD, Development and antibiotic
                     global regulator, confidence: weak, score: 19.55"
                     /tool="antismash"
     misc_feature    complement(9895..9908)
                     /note="TFBS match to BldD, Development and antibiotic
                     global regulator, confidence: medium, score: 20.99"
                     /tool="antismash"
     misc_feature    complement(9986..9999)
                     /note="TFBS match to ArgR, Regulator of arginine
                     biosynthesis genes, confidence: weak, score: 16.21"
                     /tool="antismash"
     gene            complement(10097..10759)
                     /gene="msrA"
                     /locus_tag="A4R25_RS19850"
     CDS             complement(10097..10759)
                     /EC_number="1.8.4.11"
                     /GO_function="GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA]"
                     /GO_function="GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /GO_process="GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /gene="msrA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020215983.1"
                     /locus_tag="A4R25_RS19850"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="WP_064447692.1"
                     /transl_table=11
                     /translation="MFLRRMKAEMISPDQALPGRPIAMPIADRHEVLPSTLKGPFPEGA
                     QVAVFGMGCFWGAERLFWTLPGVITTSAGYAGGYTTNPTYEEVCSGMTGHAEVVQVVYD
                     PSKIAYEDLLKVFWENHDPTQGMRQGNDVGTQYRSAIYTTTDEQAAAAKASRDAFAPIV
                     ARAGKGEITTEIAPLGDYYFAEDYHQQYLAPTKNPNGYCNHGPNGLSCPVGVARTAG"
     gene            complement(10836..11249)
                     /locus_tag="A4R25_RS19855"
     CDS             complement(10836..11249)
                     /EC_number="2.1.1.-"
                     /GO_function="GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013474065.1"
                     /locus_tag="A4R25_RS19855"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="HIT family protein"
                     /protein_id="WP_064447693.1"
                     /transl_table=11
                     /translation="MSGCVFCGIVAGEVPAFRVTDEPDGVAFLDTRPVFKGHVLVVPRT
                     HLVTLSDLPPDALPGYFRLVQRIAVAVETGLDAGGTFVAMNNRVSQSVPHLHTHVVPRT
                     KGDGLRGFFWPRTRYAGDAEAEAFAGRISAALA"
     gene            complement(11374..12066)
                     /locus_tag="A4R25_RS19860"
     CDS             complement(11374..12066)
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF020419.5"
                     /locus_tag="A4R25_RS19860"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="WP_158559962.1"
                     /transl_table=11
                     /translation="MDELHTRLLHVGFRAGDDLGLVLAGGYALAAHDLVSRPSRDIDFA
                     TATALPLPLVAARLAEAYRAGEFGCRVVEATPRMARLLVEGQGFACEVDLLKEAIGPPA
                     RLQIGPVLALEDAIGLKVRALHDRAAHRDYIDVCAASRIVEFTGLESLGARHTVAFSLH
                     ELADRLGGVDELDVETFASYGLGEDEIGGLRRWARRWEADIRARLANGETGPTGIPDDD
                     WDAYLDRA"
     gene            complement(12069..12395)
                     /locus_tag="A4R25_RS19865"
     CDS             complement(12069..12395)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018787517.1"
                     /locus_tag="A4R25_RS19865"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_064447694.1"
                     /transl_table=11
                     /translation="MSTTEALPSRLHGVADRIPTSLDDLKGPERGRVTLPTRLAWSGLS
                     EFDVDDSGQRLTLYRTLMDCGQREDIVRYVNPALLRQDWSRIRKLTAHRVIRLWESRMP
                     GLAA"
     gene            12496..13638
                     /locus_tag="A4R25_RS19870"
     CDS             12496..13638
                     /EC_number="2.5.1.48"
                     /GO_function="GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /GO_process="GO:0019346 - transsulfuration [Evidence IEA]"
                     /NRPS_PKS="Domain: Aminotran_1_2 (46-186). E-value:
                     4.3e-09. Score: 27.9. Matches aSDomain:
                     nrpspksdomains_A4R25_RS19870_Aminotran_1_2.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_1_2"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) DegT_DnrJ_EryC1"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_5"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1168: O-succinylhomoserine sulfhydrylase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011904831.1"
                     /locus_tag="A4R25_RS19870"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="cystathionine gamma-synthase"
                     /protein_id="WP_043326515.1"
                     /sec_met_domain="Aminotran_1_2 (E-value: 3e-08, bitscore:
                     27.9, seeds: 48, tool: rule-based-clusters)"
                     /sec_met_domain="DegT_DnrJ_EryC1 (E-value: 1.6e-09,
                     bitscore: 32.2, seeds: 38, tool: rule-based-clusters)"
                     /sec_met_domain="Aminotran_5 (E-value: 1.2e-06, bitscore:
                     22.4, seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRHGFDTLAIHAGQAPEARTGAVIPPIYQTSTYAQDAVGAPREGY
                     EYSRSGNPTRDALQECLAALEGGQVALAFASGLAAEDTLLRTVCKPGDHVVIPDDAYGG
                     TYRLFAKVAERWGLDYTPAKVSDPDAVRAAIRPGATRIVWVETPTNPLLGIADIAALAA
                     VAHDAGALLVVDNTFASPYLQQPIAFGADVVVHSTTKYVGGHSDVVGGALVAADRALGE
                     ELRYHQNAMGAINGPFDAWLTLRGIKTLGVRMDRHCDNAERIAAYLDGHAKVAQVIYPG
                     LPSHPGHEVAAKQMRRFGGMISFRAAGGEEHAVDICNRSRLFVLAESLGGVESLIEHPG
                     RMTHASAAGSPLEVPGDLVRLSVGIETVDDLLADLEQALG"
     aSDomain        12634..13053
                     /aSDomain="Aminotran_1_2"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_A4R25_RS19870_Aminotran_1_2.1"
                     /evalue="4.30E-09"
                     /label="A4R25_RS19870_Aminotran_1_2.1"
                     /locus_tag="A4R25_RS19870"
                     /protein_end="186"
                     /protein_start="46"
                     /score="27.9"
                     /tool="antismash"
                     /translation="YSRSGNPTRDALQECLAALEGGQVALAFASGLAAEDTLLRTVCKP
                     GDHVVIPDDAYGGTYRLFAKVAERWGLDYTPAKVSDPDAVRAAIRPGATRIVWVETPTN
                     PLLGIADIAALAAVAHDAGALLVVDNTFASPYLQQP"
ORIGIN
        1 gcccaccgtg ccggccagca gcgacagggc cgccgcccgg ccccaccggg ccacctcgtt
       61 ccgctcgctc atgacagcac tccgcttcgc tccgtgccgt catgaggcac gtttgcgctg
      121 agctggccga ttcgctcgct gcgctcgctc atgacagcac tccgcttcgc tcgctcatgc
      181 cgtttcctcc agggtggccg agtcggcggc gctgccggtc gggggcagcg cgccgacgcc
      241 gaagaaggcc cgtcggcgaa gctgcgccca gcgtccgccg ggcccgcggt cgcgaccccg
      301 gagctgggcg ccgctgacct ccgtgccggc gtgccgggcc gcctcgtgag ccgcctccgg
      361 gagcgaccgt acgccttcca cgccgggcag gacaggcctg cgttccggga ccgcgcccag
      421 cgcggacagc acggtgggca gcaacgccgc cgcggctatc gcgtacccct cggcggaggg
      481 gtggaaccgg tcccaggcga acatccgggc gggctcggcg gcgaaccgcg gcccgagcat
      541 gtcgccgagt gagacggccc ggccgccggc ctcgaccacg cccaccgtct gcgcggccgc
      601 gagctggcgg ctccagcgcc gggccagcca gcgcagcggc ggctggatcg gccggatggt
      661 gcccaggtcg ggacaggtgc cgacgaccac ctcgcagccg gccgcccgca gcgtgcggac
      721 cgcgtcgacc aggtagcgca cggccagccc cggcggcgtg cggttggtga cgtcgttgcc
      781 gcccaccagg atcaccgcca cgtccggctc gacctccagg gcggactcga cctgcggttt
      841 cagcccggac gagagcgcgc ccaccacggc gaaccggtgc aggcgtaccg gccggtgcag
      901 gcggcgggac agcccggtgg ccagcagcga gcccggtgtc tcccggcgcc tgtgcacgcc
      961 gtagccggct gccgaggagt cgcccaggat gaccatggtg accggcgggc cgggcagctt
     1021 cgcgccgtac acgccgtcgc agcgcggtgg cggggcctgc gccatcggga tggtgcgccg
     1081 ggcctgccgg gcctggccga gcagcacccc gccggtggcg gcggtggccg ccaccgtggc
     1141 gcccgtgccg atcgccgcga ggcgggcgat ctgccggacg cgctgccagt ggaccggcgc
     1201 gacggaacca gcgtccccca tgaaccgaca gtatcgcgcc ggtacgacca ctgctgtcct
     1261 tgatcggatg gctgggcggc gcccggactg ggtaccgatg ggggtggcgg acgcgggctt
     1321 gcccccgggc cgcaccatgg tcgggcggag aggggcgaag acatggggaa gacgttgaag
     1381 cgcagcgccg cgttcgccgc gctggcccgg gcgctggcgg ccggtacgcg gggcgggccg
     1441 tcgctcgggg cccggctggc ggcgctgccg aggatgatcc gcgccaccac caggggcgag
     1501 tacgacggcg gcctgcgcct ggcgctgatg gccggcgcga ccgcgtacat cgtcagcccg
     1561 atcgacctgc tgccggagat cccgctggcg atcttcgggc tggccgacga cgcggtgatg
     1621 gtcacctggc tggccggcag cgtgctcgcc gagacggaac ggttcctgga gtgggaggca
     1681 cgtcgcagct cggtcatccc ggggcatgtg gtgccctgac ggcacgtacg ctggtcggcg
     1741 aactgacggc tgaccgaccg ccccacgacg gcggcgcgac gaagggcaca acgaggtgca
     1801 gtactacgac aacgtcgtcg agctgatcgg caacaccccg ctggtccgcc tgcgcaacgt
     1861 caccgagggc atccaggcca ccgtgctggc gaaggtggag tacgtcaacc cgggcggctc
     1921 ggtcaaggac cggatcgcga tgcgcatggt ggaggacgcc gagaaggcgg gcatcctgcg
     1981 gccgggcggc acgatcgtgg agccgaccag cggcaacacc ggcgtcgggc tggccctggt
     2041 ggcccagctc aagggctacc gctgcgtctt cgtctgcccg gacaaggtca gccaggacaa
     2101 gcaggacgtg ctgcgcgcgt acggcgccga ggtggtggtc tgcccgaccg ccgtcgcgcc
     2161 ggaggacccg cgctcctact acaacgtgtc cgaccggctg gcccgggaga tccccggcgc
     2221 gtggaagccc aaccagtaca gcaacccggc caacccgcgc tcgcactacg agaccaccgg
     2281 cccggagctg tggaagcaga ccgagggcag gatcacccac ttcgtggcgg gcgtcggcac
     2341 cggcggcacc atctccggca tcggccgcta cctcaaggag gcgtccgagg gccgggtcaa
     2401 ggtgatcggc gcggacccgg agggctcggt ctactccggc ggaaccggcc ggccgtacct
     2461 ggtcgagggc gtcggtgagg acttctggcc ggagacgtac gaccggaaga tcgccgacga
     2521 gatcgtcgag gtgtcggaca aggagtcctt cgagatgacc cgccggctcg cccgcgagga
     2581 gggtctgctg gtcggcggct cctgcggcat ggcggtggtg ggcgcgctgg aggtggcccg
     2641 caaggccggc ccggacgacg ttatcgtggt gctgctgccg gacggcggcc ggggctacct
     2701 gtcgaagatc ttcaacgaca agtggatggc ccggtacggc ttcctggacg actccggcac
     2761 cgagccgacg gtggccgacg cgctcgccgg caagcccggt ggcctgcccg agctggtgca
     2821 cgtgcacccg accgagacgg tccgcgacgc gatcgactac atgcgcgagt acggcgtctc
     2881 gcagctcccg gtgctcaagg ccgagccgcc ggtggtgacc ggtgaggtgg ccggttcgat
     2941 cgccgagaag gacctgctcg acgcgctgtt caccggccag gcccacctgc acgacacgat
     3001 cgagcggcac atgggcgagc cgctgccgat gatcggcggc ggccagccgg tgagtgaggc
     3061 ggtcgggctg ctggagaagg cggacgcggc gctggtgctg gtcgacggca agcccaaggg
     3121 cgtgctgacc cgccaggacc tgctggccca cctcggcgcc cgctgaccct gcttgtaagg
     3181 aagggccccc ttttaacacc gcggtgttaa aagggggccc ttcctctacc ggaggcgtta
     3241 ataaggggcc cttccttaca agcggcgggc gtagcggagc tgggggacca gcacccggcc
     3301 gatgtgttcg tcccgctcat caccggtggc ggtccagccg ccgcgctcgt agaaacggcg
     3361 cgcgtgggcg ttgtcccgca gcacccacag caccgcgcgc gtccagcccc gggcccgcat
     3421 cgcgtcgagc gcgtccacca tcagcgcccg tccggtgccc cggccccgct cggacggctc
     3481 caggtggatc gcgttgagca gcccggtcgc cgggtcgccc tcgtcgtcgg gcccgaggta
     3541 gctgaagccc accagcttcc cgtcccgctc ggccaccgtc atccggtggt ccgcgccctc
     3601 ccaggtcagc cgttccaccc agtagcggcc catcgcctcc ggcgtcgggt cggccagcgc
     3661 ctcgggcggc aggaacgacg agtacgccgc cacccgggaa cgctggtgca gggcgccgac
     3721 cgccatcagg tcggcctcgg tcgcggaacg tagggagatc gtcacccggg cgacggtacc
     3781 cgccggggcg gcacctgtac cgtctgaaga tcctcggcga tcaccagatc accggagaag
     3841 tgctcgcggg cctcgtcgtg gaagcggcgc gggtcggcgt accgctggga gaagtgggtg
     3901 agcacgagcg tgcgtacccc cgactcggcc gcgacccgcg cggcctggcc ggcggtgagg
     3961 tgcccgacct cggcggcgag cgccgcctcc gactccagga acgtcgactc gatcaccagc
     4021 aggtcggcgt gctcggcgag cgcgtacacg ccgtcgcaga gcccggtgtc catcacgaac
     4081 gcgaaccgct gccccggccg gacgacgctc acctcgtccc gggtgacgcg gcgcccgtcg
     4141 cggtccaggt ggccgtcgcg cagcaaccgc cccacgtccg gaccggcgat gccgtacgcg
     4201 gccagccgct ccggaagcat ccggcagccg tccggctcga ccagccggta tccgtacgtc
     4261 tcgatcgggt gccgcagccg gcgggcctcc agcgtgccga cgccgagcgt gatccgctgc
     4321 ccgtcgacgt cgatcggctc gactgccagt tcggcggtct cgtagaagct ggaggcgtgg
     4381 cgcagccggg cgaagtactc ggcgccgccg gccgggaagt gcacagccac cgggcgcggt
     4441 acgcggtcca gcgagagccg ctggatcatg ccgggcagcc cgaggcagtg gtcgccgtgg
     4501 aagtgggtga cgcagatccg ggtcaggtcg gtggcggtga ccccggtgtg cagcatctgc
     4561 cgctggctgc cctcgcccgg gtcgaagagg atcacctcgt cgtcccagcg cagcaggtag
     4621 ccgttgtggt tgcgctgccg ggtcggggcc tggctggccg taccgagcac caccagctca
     4681 cgcatggaca ccgcaccctc ccgagcgcaa aaagcgaccc ccggggcttc cgcgctcgcg
     4741 cgcgggccgg ctggactggg ccggcacccc gggggtcggg tggctgtcgt ggtttcgccg
     4801 caccgctgcc acacggtctc gacgatcctg caggtgcccg cctcgcggcg ggcgagattt
     4861 cactgtcgag gacagcggct agcggacagc cacctcacga gtccgattgc tatacaagtc
     4921 tggaccacct cctttcccgt gtaccgccac gctatccact tcgcggcggc cgcggcaacg
     4981 gatttcgatc acaggcccgg tcagtccagg ccgatcgggc cctcgcgcgg gccgtcctcg
     5041 ttcgccggct gcacggcgag cgacgggaag tcggccaggt cgccctccgg ctcggcgtcc
     5101 cactcgggga acaccagcgg gctctgcagg tagaggcaga gcagctgggt gagctggtgc
     5161 aggccgtcga ggtgggtgcg ctcgtggccg tgggtggcgt ccacgccgaa cccgagcagc
     5221 gccacccggg cgtgcgcccc ggcctcgacc gcggcggcca cgtccgagcg gtagtagtcg
     5281 aacacgtccc ggaccaggtc gacgccgtgt tccttggcga tcgaggcgag gttgcgggtc
     5341 aggtggtagt cgaacgggcc gaccccgtcg cccatggcga gtgtcgccgc ggtctcccgg
     5401 gactgctggc ccggcgcgac caccgccgcg tccaccgaca cgatctccgc gacgtccggg
     5461 tccagcccgt ggctcgcgcc gtggccgatc tcctcggtga cggtgaccag caggtgcgcg
     5521 gtgaccgccg gggtgactcc ggcgtcgacc atcgccttga acgcggtcag caccgccgcc
     5581 acgcccgcct tgtcgtccag gtggcgggac ttgacgtacc cgttcggggt gatggtgggg
     5641 ttgggcagga acgcgacgaa gtccccggcg tcgatgccga gcgcccgcag cccggcctcg
     5701 tcgtgcaccg gctcgtccac ccgcacctcg accagttccc agccgacgcc ctgcaggtcg
     5761 accgcctcgt tgtagcggtg cccgctcgcc ttcagcggca gcacctggcc ggtgacgacc
     5821 cgctccaggt cgtcggtgaa gatccgcacg tgggcgccct cggcgaaacg ggcgctgtgc
     5881 gtaccgatcg ggttgacctc cagccggccg ttctccttga gccgcttcac catgccgccg
     5941 atggtgtcgg tgtgcaccac gatcgcccgg tcggcgccgg tcgaccgggg gccgggcaga
     6001 caggcgctca gcgccccgcg acgggtcagc gtggagctga tcccgatcgc ggagagccgc
     6061 tcgcccacgt actgctggat gtggtcggtg cgccccgacg ggctggggat ctccagcagc
     6121 tccaccagca cctggcggag gtagtcgagg tcgagcttga gcggttgggg cttgcgtgcg
     6181 gtcatgcccc agagcttgcc gcaccggccg gcgaccagag tcgctgcggt gcccgggtac
     6241 ccgggaagag caggtcgacg aagcgctcgg cggtgggctg cggttcgtgg ttggccagtc
     6301 cgggccgctc gttcgcctcg atgaacacgt gttccggccg gtccggggcg gggaccagca
     6361 ggtccagccc ggtgaccggg atgtccagcg cccggctcgc ggtcacgcac gcctcggcga
     6421 tcgccgggtg cagctcggcg gtcacgtcgt ggatcgtgcc gccggtgtgc aggttggccg
     6481 tccggcgtac cgcgagcacc tcgccctccg ggagcacgtc gtgcatccgg tacccggcct
     6541 cggccaccac ctcgcgggtc atgtcgtcga tcgggatgcg ggactcgccc ccggtggcgg
     6601 cggcccggcg gcggctctgc cgctcgatca gctcggtgat gtcgtgcacc ccgtcgccgg
     6661 tgatctgcgc cgggcggcgt accgcagcgg cgaccacctc gtggtcgatc accacgaccc
     6721 gcaggtcctc gcccgcgcgc agctcctcga tcagtacgtc cgggcagaac cggcgggcca
     6781 gctcgacggc cgcggtcagc gcctcgggcg tacgcacgcc gacggtgatg ccgttgccct
     6841 gctcgccccg cgccggcttg accaccaccg gacccacgtc gttcaggaac gccacgtcgt
     6901 cgggctcacc ggtggcggta cgcccgcgcg gcaccgacag gcccgcctcg gtgaggatgc
     6961 gccgggtcac ccgcttgtcg tcgcagcggc tcatcgccac cgccgaggtc agctccgaca
     7021 gcgactcgcg ggtgtggatg gtccggccgc cggtggtcag cttcagctca ccccagtgcg
     7081 ggtcggtcac ctccacccgg atgccgcggc gcatcgcctc gtccgcgacg atcttcgcgt
     7141 acgggttcag ctcgtcgtac ccctcgggca tggcgggcag gaacaggcgc tcgttgatcg
     7201 ggttcttccg cttcacgcag agcgtgccgg tgcggtggaa gccgaggcgc tcgtacagcc
     7261 ggatcgcgcc cgagttctca gccagcacgg acaggtccac gaacgcccgg ccgcgcccgt
     7321 cgagccggtc ggcgagcgcg gtgagcagcg cctgcccggt gccgggcggg gcggtgttga
     7381 agtcgactgt gaggcaccac aggctcgcgc cgttctccgg atcgccgaac accgcgacgt
     7441 ggtccacgcc ggtgatggtg ccgaccacct cacccgtggt gtcctcggcc accaggtgca
     7501 ggaaccggtc cgtgccggcg ttgtccacca gcacctcgac cggggcggtg accatgccgt
     7561 tgcgcgcgta gatccggttc accgcctccg cgtcgacggc gtcacgcagc ggccggacgg
     7621 tcagcccggg cacctcgccg ccctcggtcg cggcgggccg cttctcgccc agcggcagcc
     7681 ggtacgtcag cgacgggtcg atgaacagct cgtccggaag ccgcgacacc agcacgtgcg
     7741 ggtcgcgcag gtagatgcag atgtcccggg cgccgaccgc ctcggagcgc agcacgtcgg
     7801 ccacgtgcac ctggtcgtcg aacgtctgcc cgaacaccag ccggccccag ccgcagtcca
     7861 gcaccacgcc cgaggcgtcg gtggacgtgg gctccggatg ccgctgctcc ggcgtacccg
     7921 gtgcgaccgg gtcaccgccg gggccgatgc gttcccggcg gcgccccagc acccgttccc
     7981 ggtcggtacg tgccgttccg gtggcgaggg tgtcggtcac ggtcagtcga ttccgtggct
     8041 ctggagccac atttccagga gtcccagttg ccacagcttg tttccgttca acggggtcag
     8101 ttcggcgttc gggtcggcga gcagcgcgtc gacgtagtcg gcgcggaaca ggtcgcgacg
     8161 gcgcgcctcg ggcgcggtga gcgcgtcgcg tacccggtcg aggagcttgc cctccaggtg
     8221 ggtgagtccg ggcaccggga agtagccctt gggccggtcg atgacctcgt ggggcaggac
     8281 ccgccggccg atctccttga gcacgccctt gccgccctgg gccagcttca gctccggcgg
     8341 gcagctcgcg gccagctcga cgaactcgtg gtcgaggaac ggcacccggg cctccagccc
     8401 gtgcgccatg gtcatgttgt cgacccgctt caccgggtcg tcggtcagca tgacctgggt
     8461 gtcgatccgc aggccggcgt cgacggcggt ctgcgcgccc gggcggccca ggtgcgcggc
     8521 gacgaactcc cgcgccgggt cgccgtcggc cagccacgcc gggttgagca cccgggccag
     8581 tccggccgcg tcgcggtcga agaacgcctt cgcgtacgtc tcgagcgcct gctcccggtc
     8641 caccccggcc agcggcgggt accagtggta gccgcccagg atctcgtccg cgccctgacc
     8701 cgactgcacc accttgacgt gcttcgcgac ctcctggctg agcagccaga acgcgacgca
     8761 gtcatggctg accatcggtt cgctcatggc cccgacggcg gactccaacg gcggcagcag
     8821 gtcgccggtg ggcacccgga tctggtggtg gtcggtgccg aacgtcttcg cgaccaggtc
     8881 ggagtagacg aactcgtcgc cctcccgccc gcccaccgcg tcgaagccga tggagaacgt
     8941 ggccaggccg gaccgggcct gcccctcgcc ggcgagcagc gcgacgacaa ggctggagtc
     9001 gaggccgccg gagagcagca cgcccaccgg aacgtcggcc accatgcggc ggcgtacggc
     9061 ggtggtcagc gagtcgagca gcgcgtcctg ccagtcccgc tcggaccagc cggcccgctc
     9121 ggcggcgcgg ccgaacgacg ggtcccagta gacccgctcg tgggtggagc cgtccgcctc
     9181 gtacacccgg acggtggccg gcggcagctt ggtgacgccg gcgaggatgg tgcgcggcgg
     9241 cggcacgatg ctgtggaagc tcaggtagtg ggcgagcgcc acccggtcga tggtggtgtc
     9301 gatgccgcca ccggcgagca gcgccggcag ggtcgaggcg aaccggacca cgccggggct
     9361 ctcggccagg tagagcggct tgatgccgag gcggtcccgg gccagcacca ggcggccggt
     9421 gtcgcgctcg ctgatcgcca cggcgaacat gccgatcagg tggtcgacga agtcgagccc
     9481 ccactcggcg tacgccttga ccactacctc gctgtcgccg gacgagaaga accggtggcc
     9541 cttggcctgc agttcggcgc gtagttcccg gtagttgtag atgcagccgt tgaagacacc
     9601 ggtgagcccg gcggcggcgt ccacaatggg ttggccgctc gccgcggaca ggtcaatgat
     9661 cttcaggcgc cggtgtccca gcgcggtcgg cccttgcgac cagacgccgc tgtcgtcggg
     9721 ccctcggtca ctcatcgtgg ccgccatccg ctccacggcc gagacatcgg cgcgtgaacc
     9781 gtcacggcgg aactctcccg caagtccgca catacgagcc aatgctgcca gaggttgttt
     9841 cggttcgcct gttgagacga tgacgaacgt gtgacagctt gctacgatgc gccgtcacgc
     9901 agcgtggttg ccgcgcacag cgtcgcttcc tgccccccag ggcgcggatg ccggaaatcg
     9961 ggcccgtgcc ggctgtgagg aacgccgcat gcccgtccag ccgcagccgg aatccgcgat
    10021 cttggagttg tggtgcctgt gatgccggtt tcgcgagggt tgccaggcac cacaactcca
    10081 agatcggtgg aggggtctag ccggcggtgc gggccacgcc gaccgggcag ctcagcccgt
    10141 tcggtccgtg gttgcagtag ccattcgggt tcttcgtcgg cgccaggtac tgctggtggt
    10201 agtcctcggc gaagtagtag tcgccgagcg gcgcgatctc ggtggtgatc tcacccttgc
    10261 cggcccgggc cacgatcggc gcgaacgcgt cccgcgacgc cttcgcagcg gcggcctgct
    10321 cgtcggtggt ggtgtagatc gccgagcggt actgggtgcc cacgtcgttg ccctggcgca
    10381 tgccctgggt cgggtcgtgg ttctcccaga agaccttgag caggtcctcg taggcgatct
    10441 tcgacgggtc gtagaccacc tggaccacct cggcgtgccc ggtcatgccc gagcacacct
    10501 cctcgtacgt cgggttggtg gtgtagccac ccgcgtagcc ggcggacgtg gtgatcacgc
    10561 cggggagggt ccagaacagc cgctcggcgc cccagaagca gcccatgccg aagacagcga
    10621 cctgcgcgcc ctcggggaac gggcccttca gcgtcgacgg cagcacctcg tgccggtcgg
    10681 cgatcggcat cgcgatcggg cggcccggca gggcctggtc aggagagatc atctcggcct
    10741 tcatgcggcg gaggaacacg gtgggactcc tctcttggct ttcccagcgt gtgcaacagc
    10801 gtggccctcc ggttccttac ccgcccaccg cgacgtcagg ccagcgccgc cgagatccgc
    10861 ccggcgaacg cctcggcctc ggcgtcacct gcgtaccggg tacgcggcca gaagaagccg
    10921 cgcagaccgt cgcccttcgt cctgggaacc acgtgggtgt gcaggtgcgg tacggactgg
    10981 gacacccggt tgttcatcgc cacgaacgtc ccaccggcgt ccagaccggt ctccaccgcc
    11041 acggcgatgc gctggaccag ccggaagtag ccgggcagcg cgtcgggcgg caggtcggac
    11101 agggtgacca ggtgggtgcg gggcaccacg agcacgtgcc ccttgaacac cgggcgggtg
    11161 tccaggaacg cgaccccgtc cggctcgtcg gtgacccgga acgcgggcac ctcgcccgcc
    11221 acgatcccgc agaacacgca cccgctcatc cgcccaccct aggtccgacc cgcccacccc
    11281 acctccgggc gagcgttgat catgaggttg gcggcgacaa ttcggacggc gaccaccgtc
    11341 aacctcatga tcgacgagaa gaccggccgt aggtcaggcg cgatcgaggt aggcgtccca
    11401 gtcatcgtcg gggatgccgg tcgggccggt ctcgccgttg gcgaggcgcg cccggatgtc
    11461 cgcctcccac cgacgggccc agcgcctgag tccgccgatt tcgtcctcgc cgagaccgta
    11521 ggaggcgaat gtctccacgt cgagctcgtc gaccccgccg agtcggtcgg ccagctcgtg
    11581 aagcgaaaac gcgacggtgt gccgggcgcc caaggattcg agtcccgtga actccacgat
    11641 ccggctggca gcgcatacgt cgatgtagtc gcgatgcgcg gcgcggtcgt gaagcgccct
    11701 gaccttgagg ccgatggcat cctccagcgc gagcaccggg ccgatctgca acctcgccgg
    11761 cggcccgatc gcctctttca agaggtcgac ctcgcaggcg aaaccctgcc cctcgaccag
    11821 caaccgggcc atccgcggcg tcgcctcaac gacccggcag ccgaattctc cggctcggta
    11881 cgcctcggcc agccgcgccg ccacgagggg cagcggcaac gcggttgcgg tggcgaagtc
    11941 gatgtcgcgg gacggtcggc tcacgagatc gtgcgcggcg agtgcgtagc cacccgcgag
    12001 taccagaccg aggtcgtcac ccgcacggaa tccgacgtgc agcaagcgcg tgtgcaactc
    12061 gtccacggct aggctgccag gcccggcatt ctgctctccc agagtcggat cacccggtgc
    12121 gcggtcagct tgcggatgcg ggaccagtcc tgccggagga gcgccgggtt gacgtaccgg
    12181 acgatgtcct cgcgctgccc gcagtccatc agcgtgcggt agagcgtgag ccgctgtccg
    12241 ctgtcgtcga cgtcgaactc gctgagcccg gaccacgcga ggcgggtcgg cagcgtgacc
    12301 cggccgcgct cgggcccctt gaggtcgtcc agtgacgtgg ggatccggtc ggcgacgccg
    12361 tgcaggcggg aggggagcgc ctcggtcgta ctcaccggac cagtatggcc gaccaccccg
    12421 acagagtcct ggttgtgaca ggcgcgcccg gcctaccggg agcaaagggg aagcgggcgg
    12481 actaacgtct gcggcatgag gcacggcttc gacacactcg ccatccacgc cggtcaggcc
    12541 ccggaggccc gcaccggcgc ggtgattccg ccgatctacc agaccagcac gtacgcccag
    12601 gacgccgtcg gcgcgccgcg cgagggctac gagtacagcc gctccggcaa ccccacccgg
    12661 gacgcgctcc aggagtgcct ggccgcgctg gagggcggcc aggtggcact cgccttcgcc
    12721 agtggcctgg ccgccgagga cacgctgctg cggaccgtct gcaagccggg cgaccacgtg
    12781 gtgatcccgg acgacgcgta cggcggcacc taccggctgt tcgccaaggt ggccgagcgc
    12841 tgggggcttg actacacccc ggcgaaggtc tccgacccgg acgccgtgcg cgcggcgatc
    12901 cgcccgggcg ccacccggat cgtgtgggtg gagacgccga ccaacccgct gctcggcatc
    12961 gccgacatcg cggcgctggc cgccgtggcg cacgacgccg gcgcgctgct ggtggtcgac
    13021 aacacgttcg cctcgccgta cctgcagcag ccgatcgcgt tcggcgcgga cgtggtggtg
    13081 cactccacca ccaagtacgt cggcggacac tccgacgtgg tcggcggcgc gctcgtcgcg
    13141 gcggaccggg cgctcggcga ggagctgcgt taccaccaga acgcgatggg cgcgatcaac
    13201 ggcccgttcg acgcctggct caccctgcgc ggcatcaaga ccctcggggt acggatggac
    13261 cggcactgcg acaacgccga gcggatcgcg gcctacctgg acggccacgc gaaggtcgcg
    13321 caggtcatct acccgggcct gccctcgcac cccggccacg aggtcgccgc caagcagatg
    13381 cggcggttcg gcggcatgat ctcgttccgc gcggcgggcg gcgaggagca tgccgtggac
    13441 atctgcaacc gatcccggct gttcgtgctc gccgagtcgc tcggcggcgt cgagtccctg
    13501 atcgagcacc cgggccggat gacacacgca agcgctgcgg gctcgccgct tgaagttccc
    13561 ggcgatctcg tgcgactgtc tgtcggcatc gagacggtcg acgacctgct cgccgatctg
    13621 gagcaggccc tgggctgacc gctggctggg gagggtggca gtgccggaga tcttgccgac
    13681 gacgtgggtc ggtgcgaccg cgaaacagat cgcccgggcg gtacgccgtg gcgacgtctc
    13741 cgccacccag gtcgtcgccg accacctgga tcacgtcacc cgggtcgacg ccgacctggc
    13801 cgcgttccgc acggtacgcg gcggtgaggc gatcaccgag gcggagaagg tcgacgagca
    13861 ggaggaactg gcgaacctcc cgctcgccgg cgtgccggtg gcggtcaagg agaacacgcc
    13921 ggtggccggc ctgcccacgt ggaacgggtc ggccgccatg cgtacaccgg tggcggaggc
    13981 cgaccacgag gtggtccggc ggctgcgcgg agcgggcgcg gtgatcctgg gcgtgacccg
    14041 gatgccggaa ctgggcctgt gggcgctcac cgacgacgag accgctgcca cccgcaaccc
    14101 gtgggatccg gcgcgtaccc ccggcggctc ctccggcggc gcggccgccg cggtggccgc
    14161 cgggctggtg ccgatggcgc acggcaacga cggcctcggg tcgatccgca tcccggcggc
    14221 ctgctgcggc ctggtcgggc tcaagcccgg ccggggcgtg gtgccctgcc agctcggcgc
    14281 ggacgactgg ttcggcctga ccgagcacgg catgctcacc agcaccgtgg ccgacgcggt
    14341 ggtcggtttc tcggtgctgg ccggccgccg gccggacaag ctggtcccgc cggcgcggct
    14401 acgggtcggc gtctcgctgc gctcgccggt gcgcggcgtc tcgccggacg ccccgaaccg
    14461 ggacgcggtg gccgccgccg gccgcctgct cgccgccgcc ggtcacgaca ccgtgcccac
    14521 cgacccggtc tacccgaccc ggctcggcct gcagggcatc gccacctggt tcgcggcggc
    14581 cgccgccgac gtgcgcgccg ccgggatcgc cccgcgccag ctccagcgcc gcagccgccg
    14641 gcacgtcacg ctcggcgagt gggcgcagcg ccgggggtac gtgcgggagg ccgaccgggc
    14701 ggcctggcgc gaacggtcgg tgaacttctt cgcggacaac tcgatcgacc tgctgctcac
    14761 cccggcgctg gccgggccgc cgccggaggc cgccggctgg tccggccgct cct
//
