LOCUS       NZ_CP011152            12220 bp    DNA     circular CON 28-JAN-2024
DEFINITION  Bacillus cereus strain CMCC P0021 plasmid pRML04, complete sequence.
ACCESSION   NZ_CP011152
VERSION     NZ_CP011152.1
KEYWORDS    RefSeq.
SOURCE      Bacillus cereus
  ORGANISM  Bacillus cereus
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus;
            Bacillus cereus group.
REFERENCE   1  (bases 1 to 591110)
  AUTHORS   Wang,Y., Tian,W., Zhu,L., Wang,B., Wang,H. and Zeng,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-APR-2015) Institute for Biological Product Control,
            National Institutes for Food and Drug Control, No. 2, Tiantan Xili,
            Dongcheng District, Beijing 100050, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: RS_HGAP_Assembly v. V2
            Genome Coverage                   :: 35.03
            Sequencing Technology             :: Pacific Biosciences
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/28/2024 08:42:11
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,142
            CDSs (total)                      :: 5,983
            Genes (coding)                    :: 5,793
            CDSs (with protein)               :: 5,793
            Genes (RNA)                       :: 159
            rRNAs                             :: 16, 16, 16 (5S, 16S, 23S)
            complete rRNAs                    :: 16, 16, 16 (5S, 16S, 23S)
            tRNAs                             :: 106
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 190
            CDSs (without protein)            :: 190
            Pseudo Genes (ambiguous residues) :: 0 of 190
            Pseudo Genes (frameshifted)       :: 77 of 190
            Pseudo Genes (incomplete)         :: 106 of 190
            Pseudo Genes (internal stop)      :: 68 of 190
            Pseudo Genes (multiple problems)  :: 50 of 190
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 15:21:29
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 255136
            Orig. end                         :: 267356
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP011152.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..12220
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="2"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..12220
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..12220
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:7220](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..7220
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            38..1720
                     /locus_tag="WR47_RS28655"
                     /old_locus_tag="WR47_29335"
     CDS             38..1720
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947886.1"
                     /locus_tag="WR47_RS28655"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="WR47_29335"
                     /product="DUF2075 domain-containing protein"
                     /protein_id="WP_063549697.1"
                     /transl_table=11
                     /translation="MLSFAAKLSSFYIIKKKRKEFEQGCIRDPKIDGIPVVYIQRDKKN
                     LYVGKSTDIYERFVAHISDEAKTFNEVIVIKSELFNESSIKHIETLLIEYLSADDKFNL
                     LNKVRGQKIHSYNGIEDVQSMFKEIWNELIKEGVASENLGEIHNKFIYKYSPFKELSAN
                     QIEVCKDIFNTMLTTSNSRHLITGDPGTGKTIVLTNILYALAYDSETGKAREGLNYKDI
                     ALVVPQNHSLNLYKSLMNKLGLQSITVLSPSQFIKQAKSRGTKYKYVFVDEAHRLKQYF
                     GKQARDLKHLVTDNGYTTELELIETFAYHLTVVYDPYQTIRPADIDTDVFQQLTANYKR
                     HPLHKQFRLKSGDQYLAWLRKYLQIANNVGVYEEGLLRGYDFKVMDSITELNQSMKALN
                     EKYELCRVVAGYSWKWITQKDKNHYDIKDPVTGDTFKWNSKVKGWINFENSIEEIGCIH
                     TTQGADLNYVGVILGAEIDCDYTGEENGDYDLNKAKIVVNPKEYKDRNGLPIKGTDINN
                     GELTSYIKRIYYVLLSRGINGCYVYAVNQNMQRYLKEIVKVSQ"
     gene            complement(2021..2509)
                     /locus_tag="WR47_RS28660"
                     /old_locus_tag="WR47_29340"
     CDS             complement(2021..2509)
                     /EC_number="3.4.-.-"
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /GO_process="GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Abi"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001172082.1"
                     /locus_tag="WR47_RS28660"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="WR47_29340"
                     /product="CPBP family intramembrane glutamic endopeptidase"
                     /protein_id="WP_063549699.1"
                     /sec_met_domain="Abi (E-value: 1.8e-14, bitscore: 48.9,
                     seeds: 823, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MQKRLHHFIYNLRFPYFIILMTIATFVLPLLSFLIPIEDAQQNPI
                     EDISLIRQVISGCVVAPLIETALYQMFLFWILKDIPFVRKYDNIPTIFLSAIIFGTIHS
                     YGLSYIVYSALMGVILGYSYWIYQKKKEKTSKAPSAYWVVFLIHALHNLFTFLLKNFF"
     misc_feature    2675..2686
                     /note="TFBS match to CcpA, Catabolite control protein A,
                     confidence: medium, score: 18.77"
                     /tool="antismash"
     gene            2762..2989
                     /locus_tag="WR47_RS28665"
                     /old_locus_tag="WR47_29345"
     CDS             2762..2989
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000854591.1"
                     /locus_tag="WR47_RS28665"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="WR47_29345"
                     /product="hypothetical protein"
                     /protein_id="WP_000854592.1"
                     /transl_table=11
                     /translation="MKTLNKENSLLKNLEEIELEEINGGGWRDHLRWGADFGRGLVSFP
                     YGCGMGDAYIKGSTYTSSGGGYLKGSYGSR"
     gene            3072..4466
                     /locus_tag="WR47_RS28670"
                     /old_locus_tag="WR47_29350"
     CDS             3072..4466
                     /EC_number="2.4.-.-"
                     /GO_function="GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Glycos_transf_2"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016135757.1"
                     /locus_tag="WR47_RS28670"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="WR47_29350"
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="WP_063549701.1"
                     /sec_met_domain="Glycos_transf_2 (E-value: 1e-20, bitscore:
                     69.3, seeds: 256, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTNFNYVSKELRYNINFFEIACNKLLTMNIDDNSLLKKVKKRLAL
                     LNHIHKYSEEVNELLSELHSGTVDEINHLSDIQFIEMLKSFLVTKRIKVSVNIMTLNEE
                     RCIERCIRSIKNFADEIIVLDTGSTDKTLEIIQKKFPDVKLYCTEWKDDFSECRNQLID
                     YSTGDWIFQIDADEDLEINQEDLRDFLELFYEFPKSPMVICPKIKNHDNQELDFNKRIF
                     KKNDNLRYFGMIHEDLRYNIQKKGDDLIYFTTDFVLNHDGYKPEIIELKNKYKRNLDLE
                     NEMVRIEPDNIRWFYFLARERKLAGYSDEAVIHTLVQGIKNSKNEQKNNHFYLLSLLML
                     ADLYHNNHNFEALHGIANELSNGFPYCIDGLYYNLISSWEYQSSQISLLTEKSFQHMRK
                     VESPYSIINSNGYHIFYLLGMLYFNVGNYAKAFQMFSIIKDDIMLDEIKSNLNALKDSI
                     ENFLSK"
     gene            4569..4919
                     /locus_tag="WR47_RS28675"
                     /old_locus_tag="WR47_29355"
     CDS             4569..4919
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016135758.1"
                     /locus_tag="WR47_RS28675"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="WR47_29355"
                     /product="PH domain-containing protein"
                     /protein_id="WP_063549703.1"
                     /transl_table=11
                     /translation="MQKSLGKIKEQLDSTENILCTLQCSFDQYVNRNCARPGILVATEQ
                     RMIFYGTPFMNFKNEIVEEFLYEGISSFTLKKNIFGIRIRMQYNDEFLKFNKITNADAT
                     TFVETVNILRKK"
     gene            5001..7220
                     /locus_tag="WR47_RS28680"
                     /old_locus_tag="WR47_29360"
     CDS             5001..7220
                     /GO_component="GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA]"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /GO_function="GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /GO_function="GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_function="GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: TIGR01193"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Peptidase_C39"
                     /gene_functions="transport (smcogs) SMCOG1288: ABC
                     transporter related protein"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000251810.1"
                     /locus_tag="WR47_RS28680"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="WR47_29360"
                     /product="peptidase domain-containing ABC transporter"
                     /protein_id="WP_063549705.1"
                     /sec_met_domain="Peptidase_C39 (E-value: 1.7e-39, bitscore:
                     129.7, seeds: 36, tool: rule-based-clusters)"
                     /sec_met_domain="TIGR01193 (E-value: 6.1e-214, bitscore:
                     707.3, seeds: 5, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MVRKKFRCIKQHDLKDCGPACLAMISRHYGLTISISKIREIAGTD
                     LHGTNIKGLLTAANRLDFDTKGVKAESIDALSEIPLPAIAHVVMDGGLLHYVVIHKVKN
                     GKIYVADPAQGLVTYTSVDFGQIWQGILILLAPRQNFQKGKQSQGTLSRFFSLLRPQMS
                     LLLPLFVASIFFNIFGFAGAFYFKFLIDDIVTKQLLQTLHIVSIGIVILYIFKVLLSYF
                     RTHLILYLSRRMDIQLMLGYYHHVVGLPMNFFETRKVGEIISRFMDASKIREALSTITI
                     TLMIDTVMVLLGGILLYVQSSTLFVVTLLLIPFYIGVIFLFHKPYQTINRNEMESNAKL
                     TSYLVESLNGIATIKSYNAEKEVFFQTENRFVNLLKHTFKRGMLSNLQGSIKMVLALIG
                     GTVILWVGAIQVLKGNMTIGELITYNALLGYFLNPIENLIGIQPTMQSALVAGERLNEI
                     LDLDGEKDETEYRKVSPKALSGKIEWSDVTFRYGTRKNVLENINFSIQSGHQVAFVGES
                     GSGKTTIAKLLMNYYEPQKGDIYYDEFHIKEVNRTALRDRIAYVSQDSFFFSGSIYDNL
                     RFGLEQMLSLEEIIQACKSACAHEFICDLPLRYETHLEENASNLSGGQRQRLAIARALL
                     KKPDIIILDEATSNLDSTTEKHITDMLKDLGSKGITVIMIAHRLSTIQHANRIFVMGKG
                     NILEKGSHEELLTQKGEYYRLWKNQTVHAEYLETEFDAIRGGQYG"
     gene            7213..8520
                     /locus_tag="WR47_RS28685"
                     /old_locus_tag="WR47_29365"
     CDS             7213..8520
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1029: RND family
                     efflux transporter MFP subunit"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000041333.1"
                     /locus_tag="WR47_RS28685"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="WR47_29365"
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="WP_063549707.1"
                     /transl_table=11
                     /translation="MVKIYSFDELTDSVELLERKPPRFIAILLSFLFLCLLVFAIWAYV
                     GKVDIVSKGTAIVEGKSNISTIRTQIVGIVDTISVQSGDEVKKGETLLQLKNQELTGKQ
                     KQLNNIIKHVEEQKTMLEQLKQSIKSEKAIFSKDLDQKIRDEYKAYEQGYQALEKEQDN
                     EIKSLMNNKLPNEQDEVLQGLLAEKENIQRELKFTRKQKEKTTLEQIDSINERIQSLEA
                     QQTNVEKRVEQRKVTLENERSKIESVKEGKQEQKKFTLRKYKEDALVSINQRIQSVEQE
                     LFVKKQEMDSLHHQGETTVIKAQKDGIIQFPSILQTGDLVDPGQELISIIPKENIKKIK
                     ILMPAQEVKGIKKGDKAQYSFKLKKTDKQVGTVTYISAHPIFDKDSKTYMYEMEASIDT
                     NEFDELHMGMVGRVSVVTGEESIWKFILRELDFISN"
     gene            complement(9227..>9382)
                     /locus_tag="WR47_RS31885"
                     /pseudo=""
     CDS             complement(9227..>9382)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016090861.1"
                     /locus_tag="WR47_RS31885"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /product="zinc ribbon domain-containing protein"
                     /pseudo=""
                     /transl_table=11
                     /translation="CGYQNKDVKNLNLHEWDCPSCCTHHDRDINASINLKNEAISLLTA
                     RTVEIA"
     gene            <9384..9718
                     /locus_tag="WR47_RS28690"
                     /old_locus_tag="WR47_29370"
                     /pseudo=""
     CDS             <9384..9718
                     /GO_process="GO:0030638 - polyketide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000764204.1"
                     /locus_tag="WR47_RS28690"
                     /note="frameshifted; incomplete; partial in the middle of a
                     contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /old_locus_tag="WR47_29370"
                     /product="ester cyclase"
                     /pseudo=""
                     /transl_table=11
                     /translation="NEVFKNIISISFLNM"
     gene            complement(9933..10874)
                     /locus_tag="WR47_RS28695"
                     /old_locus_tag="WR47_29375"
     CDS             complement(9933..10874)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181487.1"
                     /locus_tag="WR47_RS28695"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="WR47_29375"
                     /product="zinc metalloprotease"
                     /protein_id="WP_063549709.1"
                     /transl_table=11
                     /translation="MSNHTNSSKKKITPTVRKCATIDLHHKLLQESEQYKNARVEIESM
                     TKEYLNSARAQEERPVVKIPVVVHVVWNTEEQNISEEQVHSQIDVLNLDFRAANPDIAN
                     VPSAFKDSVSDAKVEFFLATKDPNGNQSNGITRTHTTKQSFSVVDDPVKSQSTGGINAW
                     PADEYLNIWVCHLEDGLLAYAQFPGGPPKTDGVVIAYNYFGTTGTATEPFHLGRTATHE
                     IGHWLNLFHIGGDDGGACNGTDRVADTPNQADLNVGKPTFPTISCNNGPNGDMFMNYMD
                     FVDDEIMLMFTQGQVDRIDACLAGPRSSFLTN"
     gene            complement(11222..12163)
                     /locus_tag="WR47_RS28700"
                     /old_locus_tag="WR47_29380"
     CDS             complement(11222..12163)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181487.1"
                     /locus_tag="WR47_RS28700"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="WR47_29380"
                     /product="zinc metalloprotease"
                     /protein_id="WP_063549711.1"
                     /transl_table=11
                     /translation="MTNHTNSSEKTITPTVRRCATIDLHHKLLQESEQYKNARVEIESM
                     TNEYLNSARVQEERPVVKIPVVVHVVWNTEEQNISEEQVHSQIDVLNLDFRAANPDIAN
                     VPSVFKDSVSDARVEFFLATKDPNGNQTNGITRTHTTKQSFSYLDDPVKSQSTGGVNAW
                     PADKYLNLWVCKLEDGLLGYAQFPGGPSKTDGVVIGYNYFGTTGTATEPFHLGRTATHE
                     IGHWLNLFHIGGDDDGACSGTDRVADTPNQADQNTGKPTFPTISCDNRPNGDMFMNYMD
                     YVDDDTMVMFTKGQVDRIDACLAGPRSSFLTN"
ORIGIN
        1 aagtattctt aaaattaaaa agtagggagt tattcaaatg ctatcatttg cagcaaaatt
       61 atcttcattt tacataatta aaaagaagcg aaaagagttt gagcaaggtt gtatcagaga
      121 tcctaaaata gatggaatac cagtagtcta tattcagcgg gataagaaga atttatatgt
      181 aggtaagtct acagatatat atgagcgttt tgttgcacat ataagtgatg aagctaaaac
      241 attcaatgaa gttattgtaa taaaaagtga attatttaat gaaagttcaa ttaagcatat
      301 tgaaacattg ttaattgaat atttatctgc agatgataaa tttaacttac tgaataaagt
      361 aaggggccaa aagattcatt cttataacgg cattgaagat gttcaaagta tgtttaaaga
      421 aatttggaat gagttaatta aagaaggggt agcaagtgag aatttaggtg agatacataa
      481 taaatttatt tataaatatt caccgtttaa agagttatcg gctaatcaaa ttgaggtttg
      541 taaagacatt tttaatacaa tgcttactac atcaaatagt cgacatttga ttacagggga
      601 cccgggtact ggtaaaacaa ttgtattaac aaacatctta tatgcgcttg cttatgacag
      661 tgaaactggt aaagcgagag agggattgaa ttataaggat atagcacttg ttgttccaca
      721 aaatcactca ttaaatttat ataagagctt gatgaacaaa ctaggtctac aaagtattac
      781 agtactgtca ccttcacaat ttattaagca ggcaaaaagt aggggcacaa aatataaata
      841 tgtttttgtg gacgaagcac atcgtctaaa acaatatttt ggtaaacaag cacgtgattt
      901 aaaacattta gtcaccgata atggttatac aactgagttg gaattaatag aaacatttgc
      961 gtatcactta actgtggttt atgatccata ccaaacaatt cgaccagcag atatagatac
     1021 agatgtattc cagcagttga cagcgaatta taagaggcat cctttacata aacaattccg
     1081 tttaaaatct ggagatcaat atttagcatg gcttcgtaaa tatttacaaa tagcaaataa
     1141 tgttggagtg tatgaagaag ggttattgcg gggttatgac tttaaggtaa tggactctat
     1201 tacagagttg aatcaatcta tgaaagcgtt aaatgaaaaa tatgaattat gtcgtgtagt
     1261 cgccggttat tcttggaaat ggattacaca gaaggacaaa aatcattatg atataaaaga
     1321 tcctgttaca ggcgatacat tcaaatggaa ctcaaaggta aaaggttgga ttaactttga
     1381 gaatagtata gaggaaatag gatgtataca tactacccaa ggtgcggatt taaattatgt
     1441 gggtgtgatt ttaggagctg aaattgattg tgactataca ggtgaagaaa atggagacta
     1501 tgatttaaat aaagctaaaa ttgtagtgaa cccaaaagaa tataaagatc gaaatggtct
     1561 tccaattaag ggaacagata taaataatgg ggagttaaca tcctatatta aacgtattta
     1621 ttacgtgcta ttatctcgtg ggataaatgg atgttatgtc tatgcagtaa atcaaaatat
     1681 gcaaagatat ttaaaggaga tagtaaaagt aagtcaataa ttatgaaaag ggattacaga
     1741 atttacgata cgggaaagtc catgcaggac tttagtcagc aacaggaagc ataactttgc
     1801 tgtacaagga tttaattctg taaagttttt gcaagaagtt taaactagaa taaaaattat
     1861 taaaccaata taatctcata ttaatttaaa tcaaaaaaac gctattcttc ttgtagaaat
     1921 atacagaaag aatagcgttt tttatgaatt ttttaattcg aggtgaaata taatttttaa
     1981 tttgaaaatt ccttatattg ctgttcaaat cataatctgt ttaaaagaaa ttttttaata
     2041 gaaaggtaaa taaattatgt aatgcatgga ttaaaaaaac aacccaatag gcggaggggg
     2101 ctttggatgt tttttctttc tttttttgat aaatccaata tgagtatcct agtatgacac
     2161 ccattagggc gctataaact atataggaaa gcccatatga gtgaatagtt ccaaaaataa
     2221 tggctgaaag aaaaattgtt gggatattat cgtatttacg cacaaatgga atatctttta
     2281 aaatccaaaa caaaaacatt tgataaaggg ctgtttctat tagcggggct acgacacacc
     2341 cagatataac ttgccgaata agagagatat cctcaatcgg attttgttgc gcgtcttcta
     2401 ttggtataag aaaggataac aatggtagta caaatgtagc tatagtcata agtatgatga
     2461 aataagggaa tcttaagtta taaataaaat gatgtaaacg tttctgcatg atctactcct
     2521 ttggcatgtt gtgaaattat aaatcctggt taggaactat gtattttact ttactatact
     2581 attaatcata atgatgggca aacttggaaa atgtgtactg aatatattat tctgcaaata
     2641 taatctaaga ttaatatttt gtaaaaaaac tattgaaaac gtttccttat aggtgtacaa
     2701 tgaatatcgt aagcttacaa tatatttata taatgatttt tgaaaatgga ggatgttatg
     2761 tatgaaaact ttaaacaaag aaaatagttt attaaaaaat ctagaagaaa ttgaattaga
     2821 agaaattaat ggtggaggct ggagagacca tctaagatgg ggcgcagact ttggtagagg
     2881 acttgtatct ttcccttatg gttgcggtat gggcgacgca tatattaaag gtagcacata
     2941 cacttctagt ggcggtggtt acttaaaagg atcatatggt agccgttaat aacttttatt
     3001 aataaataat agctacaggg ttagtttaca aaactaaccc tgtagctatt acattaaagc
     3061 agggatgata tatgacaaac tttaattatg tttcaaaaga attaagatat aatataaatt
     3121 tttttgaaat agcatgtaat aaattattaa caatgaatat tgatgataat agcttattaa
     3181 aaaaggttaa gaaacgatta gcattgttaa accacattca taaatattca gaagaagtta
     3241 acgaattact ttctgaatta cattcaggca cagttgatga gataaatcat ttaagtgata
     3301 tacaatttat agaaatgtta aaaagctttc tagtcactaa aagaattaag gtttctgtga
     3361 atatcatgac tttaaatgaa gaaagatgta ttgagagatg tattagaagt attaaaaatt
     3421 ttgcagatga gattattgtt ttagatactg gttctactga taaaacacta gaaatcatac
     3481 aaaaaaaatt cccggatgta aaattatatt gcacagagtg gaaagatgat ttttcggaat
     3541 gtagaaatca gttaatagat tattcgacgg gcgattggat atttcagatt gatgcggatg
     3601 aggatttaga aatcaaccaa gaggatttaa gagatttctt agaattattt tatgaatttc
     3661 ctaaatctcc aatggttatc tgtccgaaaa ttaaaaatca tgataaccaa gagttggatt
     3721 ttaataaaag gatttttaaa aagaatgata atctcagata ctttggaatg atccatgaag
     3781 atttacgtta taatatccag aaaaaagggg acgacttaat ctattttact actgattttg
     3841 tacttaacca tgatgggtat aagccagaaa taattgaatt aaaaaacaaa tataaaagaa
     3901 atctagattt agaaaatgaa atggtacgca ttgaaccaga caacatcaga tggttttatt
     3961 tcttagccag agaaagaaaa ttagctggtt attctgatga agcagtaatt catacactcg
     4021 tacaaggaat aaagaatagt aaaaatgagc aaaaaaataa tcatttttat ttattatctc
     4081 ttttaatgtt agcagatctc tatcataata atcataattt tgaagcgcta catggaattg
     4141 ctaatgaatt atctaatgga ttcccgtatt gtattgatgg attatactat aatttaatca
     4201 gtagctggga ataccagtca tctcaaattt cacttcttac cgagaaaagt tttcaacata
     4261 tgagaaaggt cgaatctcct tattccataa taaattctaa tgggtatcat atcttttatc
     4321 tattaggcat gttgtatttt aatgtgggga attatgcaaa ggcttttcaa atgttttcta
     4381 taataaagga tgacattatg ctagatgaaa tcaaatcaaa cttaaatgct ttaaaagata
     4441 gtattgaaaa ttttttaagt aaataaatag tatatcctaa ctactgtaaa tacttttatt
     4501 ttgagttagg atttatggct aagagagata gatcctaact caaaataaaa ttgtaaggag
     4561 tttttggaat gcaaaagtca ttaggaaaaa taaaggaaca attggattct actgaaaaca
     4621 tattgtgtac attacaatgt tcattcgacc aatatgtcaa ccgaaattgt gctcgtcccg
     4681 gcatattagt tgccactgag caaagaatga ttttttacgg aacacctttt atgaatttta
     4741 aaaacgaaat agttgaagaa ttcctttatg aagggatctc ttcattcaca ttgaaaaaga
     4801 atattttcgg aattcgaatc cgaatgcagt acaatgatga attcttgaaa tttaataaaa
     4861 ttacgaatgc agatgctact acatttgtag aaacagtaaa tatcctacgt aaaaagtaat
     4921 agcaaacttc gtatctgcta cttagataat aaaatgtttt gtaacttagc agttttatca
     4981 ctgagagaga aggtagtata ttggttcgaa agaagtttcg ttgtatcaaa caacacgatt
     5041 taaaagactg cggtccagct tgtttagcta tgatttcacg tcactatggc cttacaatat
     5101 ccatatcaaa aattcgagaa attgctggaa cggacctaca tggcacaaac attaagggtt
     5161 tactaacagc tgctaataga ttggattttg atacgaaggg tgtaaaagca gagagtatag
     5221 atgcattgtc agaaatacct ttgccagcta ttgctcatgt cgttatggac ggtggactac
     5281 ttcattatgt agtcatacac aaagtaaaga acggtaaaat ttatgtagca gatccagcac
     5341 aaggccttgt tacatataca tcggttgatt tcggtcagat ttggcaagga atattgatac
     5401 tactggctcc tcgtcagaat ttccaaaaag ggaagcaatc tcaagggaca ttatctcgat
     5461 ttttttcact attaagacct caaatgagct tgctacttcc actatttgta gcttctattt
     5521 tttttaatat atttggtttt gctggcgcat tttattttaa gttcttaatt gatgatattg
     5581 tgacgaaaca attgttacaa acactgcata tagtatctat aggaattgtt attttataca
     5641 tttttaaagt attactctcc tattttcgga cgcacctcat tctatattta agtagacgga
     5701 tggatattca attaatgctt ggatattatc atcatgttgt aggcttacct atgaattttt
     5761 ttgaaacccg aaaagtcggt gaaattattt cccgctttat ggatgcaagt aaaatacgtg
     5821 aagctttatc gacaattact attacattaa tgattgatac agttatggtt ttgttgggtg
     5881 gtattttatt atatgtgcaa agctctactt tatttgtagt tacattactt ctcatcccat
     5941 tttatatagg ggttattttt ttatttcata agccctatca gaccataaat cgtaacgaga
     6001 tggaaagtaa tgccaaatta acatcgtatt tagtggaatc attaaatgga attgcgacaa
     6061 taaagtccta taatgcggaa aaagaagtgt tttttcaaac agaaaatcgc tttgtaaact
     6121 tattaaaaca tacatttaaa agaggaatgt tatcaaattt acaaggttct attaaaatgg
     6181 ttttagcact tattggaggt actgttattc tttgggtggg agcaatacaa gtcctaaaag
     6241 gaaatatgac aattggcgaa ttaattacat ataatgcact tttaggatat ttcttaaatc
     6301 caattgaaaa tttaatcggt attcaaccta cgatgcaatc tgcattagtt gctggcgaac
     6361 gattaaatga aattttagat ttagatggag aaaaagatga aacagaatat cgtaaagttt
     6421 ctcctaaagc attatctggg aagatagagt ggtcagatgt tacattccga tatggaacac
     6481 gtaaaaacgt gttggagaat atcaactttt cgattcaatc aggtcaccaa gtcgcatttg
     6541 taggagaaag tggttcaggt aaaacgacta ttgctaagtt attaatgaat tattatgaac
     6601 cacaaaaagg tgacatatat tatgacgaat ttcatataaa agaggttaat cgtacagcac
     6661 taagagatcg tattgcttat gtatcacaag attcattctt ctttagtgga tctatttatg
     6721 ataatttacg atttggatta gaacaaatgc tttcgttaga agaaattatt caagcttgta
     6781 agagtgcatg tgcgcatgaa tttatttgtg atttaccttt acgctatgaa actcatttag
     6841 aggaaaatgc ttcaaatcta tctggcggtc aaagacagag attagctatt gcaagggcac
     6901 tgttaaaaaa accagatatt attattttag acgaagcaac cagtaactta gattcaacaa
     6961 cagaaaagca tattacagat atgttaaaag acttaggatc caaaggcata acggttatca
     7021 tgattgcgca tcgacttagt accattcaac atgccaatag aatttttgta atgggaaaag
     7081 gaaatatcct agaaaaagga tctcatgagg aattattaac tcaaaaaggt gagtattatc
     7141 gtctatggaa aaatcaaacc gttcatgcgg agtatctgga gactgaattt gatgcaatac
     7201 gaggtggaca atatggttaa aatatactct tttgatgaat taactgatag tgttgagtta
     7261 ttagaaagaa aaccgcctcg ttttatagct atattgttga gttttctttt tttatgtcta
     7321 ttagtttttg ctatttgggc atatgttggt aaagtagata ttgttagcaa aggtactgcg
     7381 atagttgaag ggaaatcaaa tatcagtaca attcgtacgc aaattgtagg tattgtagac
     7441 actatttctg tgcaatctgg tgatgaagta aaaaaaggag agaccttact ccaattgaag
     7501 aaccaggagt taacaggtaa acagaagcaa ctaaataaca ttataaaaca tgtagaagaa
     7561 caaaaaacaa tgttagaaca gctaaaacag agtattaaat ctgaaaaagc aattttttct
     7621 aaggatttag atcaaaaaat acgtgatgaa tacaaggcat atgagcaagg gtatcaagct
     7681 ttagaaaaag aacaggataa tgagattaaa tctctcatga ataataagtt acctaacgaa
     7741 caagatgagg ttttacaagg gttacttgcg gaaaaggaaa acatacaaag agagctaaaa
     7801 tttactcgaa aacagaaaga aaagacgaca ttagaacaaa tcgattcaat taatgaaagg
     7861 attcaatcat tagaggcgca acaaactaat gtagaaaaaa gagtcgaaca aagaaaagta
     7921 acattagaaa atgaaagaag taaaattgaa tccgttaagg aaggaaaaca agagcaaaag
     7981 aaatttactt tacgaaaata taaagaggat gctcttgttt caattaatca gcgtattcag
     8041 tcagtagagc aagaactatt tgtaaaaaaa caagaaatgg atagcttaca tcatcaaggt
     8101 gaaacgactg ttataaaggc acaaaaggat ggtattatac aatttccctc tattttacaa
     8161 acaggtgatt tagtcgaccc aggtcaagag ttaatatcga ttattccaaa ggaaaatatc
     8221 aaaaagataa agatattaat gcctgcacaa gaagtaaaag ggataaaaaa aggagacaaa
     8281 gcgcagtatt cctttaaatt aaaaaagacc gataagcagg ttgggactgt aacttatata
     8341 tctgcccacc ctatttttga taaagattcc aaaacatata tgtatgaaat ggaagcttca
     8401 attgatacaa atgaatttga tgaattacat atgggaatgg taggcagagt ttctgtcgtg
     8461 acgggtgaag aatccatttg gaaatttatt ttacgcgagt tggattttat ttctaattag
     8521 tctaaaagga gaaagggaag aactattagt aagcacgttg ataaagatat tgtagtaaga
     8581 gtttggcaac aagcagaaat ccaatgagta ttaacaattt gagaaattgg aaaaagagat
     8641 acttaatatt tttaatatga ttataacagt gcattcgttt ttgtaaatgt aacttatggt
     8701 tcttgctcat taacttggac ctctctaaca gatgcaaaaa ctgaatgtag atcctatgta
     8761 acgatgccag ttggattttt taaaaaataa caaaaggtgc atatatacta tttagtatat
     8821 gcaccttttg ttattttcgg catgaagatt gatattaaac cggttacata tcataaccat
     8881 agatagtaca attagtaatt taaaaaacaa tatttttcat gtatttctct taatgtaatg
     8941 aattttatta atattattag acttaatttc atgaaaagaa agttgaaaaa tagggttaga
     9001 aactatcttg aaaccacttg acactttata tggaaactgc atgtattatt taatgggtaa
     9061 gctatctttc aaatcataac tgcttttatt ttcaagaaat tttcggagat aataataact
     9121 attttttatg cagaattaaa agtctctttt cttgaactac ctccactttc acttcgttta
     9181 gaagaggggg attcctaagt aaagagttct atcgaactct aatttattag gctatctcca
     9241 cagtccttgc ggttagaaga cttatcgctt cattctttag attgatactt gcgttaatat
     9301 ccctatcatg gtgtgtacaa caagaaggac agtcccattc atgtaggttt agatttttaa
     9361 cgtctttatt ttgataccca caaaatgaag tttttaaaaa cataatatcg atatcattcc
     9421 taaatatgta agtgaagatt ataagcaaca taatcttttt gtggctgatg gacgaaaagc
     9481 tttcatggat ttctttaagg aggattttgt taaaaaccct aactcctctg cagaaattaa
     9541 acgtgtagtg gctgaaggag atatagttgc tcttcatgta cattctcgtg ctaactctca
     9601 agataaggga gttgctatag ttgatgtatt tcgtataaag gatggtaaga ttgtcgaaca
     9661 ctgggatgta attcaagaga ttccaaatga ggcagttaat gataatacta tgttttagat
     9721 agaagatcta tttaataatt gtttaatcga tagagatata taggatttag aaatttagac
     9781 aagtaaaata aaggaggtgt cctagacatg atactaggac atctcttttt gttttaacaa
     9841 gatttaattt actattattt atagagtact tatttcgtgt tttttgataa cttaacacta
     9901 aaactatagc gctataaaat tagataaccg catcagtttg taagaaagga tgaacgtggt
     9961 ccagcaaggc atgcatctat acgatctact tggccttgtg taaacatcaa cataatttca
    10021 tcgtcaacaa aatccatata gttcataaac atatcaccat tcggtccatt attacaagaa
    10081 attgtaggga atgtaggttt tccgacattt agatctgctt gatttggcgt atccgcaact
    10141 cgatcagtgc cattgcatgc accaccatca tcaccaccaa tatgaaatag gttaagccaa
    10201 tgtccaattt catgagtagc tgttcgacct aaatggaaag gttctgtcgc agtacctgta
    10261 gtgccaaaat agttgtatgc tataactaca ccatctgttt taggtggacc accaggaaat
    10321 tgggcgtatg ctaaaagacc atcttctaaa tggcataccc atatatttaa gtattcatca
    10381 gctggccatg catttattcc acctgtactt tgtgatttga caggatcatc tacaactgaa
    10441 aaagattgct tagttgtatg ggtacgagtg ataccatttg attgatttcc gttaggatct
    10501 tttgtagcaa gaaagaattc aactttagcg tcacttacag aatctttaaa ggcagaaggt
    10561 acgtttgcta tatcaggatt cgctgcacga aaatcaagat ttaaaacatc aatttgacta
    10621 tgaacttgtt cttcagagat gttttgttcc tcagtattcc aaacaacatg aactacaaca
    10681 ggaattttga caacgggtct ttcttcttgt gcacgtgcgc tattgagata ttccttcgtc
    10741 atactttcta tttcaacacg tgcattttta tattgttcgg actcttgtaa tagtttatgg
    10801 tgaagatcta tagttgcaca tttacgcaca gtaggtgtaa tttttttttt tgatgagttg
    10861 gtatgatttg acataagaat ttctccttta gatgttattt gaagttttag taaatatata
    10921 cggtaagtta gtactaaaaa gtgatgtgaa tgtttagtgt ttttaatttt taatgtattc
    10981 tggggtcatt atgtccatgc aggaaatcaa aatatgcaga gacacttgaa gggaatattg
    11041 cgcataagtc aagaactata aaaaggaatt tggaaattta gataggttat ttataaggtg
    11101 aataaaacta agggcatgta ctaggtacat gcccttagtt ttaatttgat atattttgta
    11161 tagtatgctt attttacgtc atgttcttat agctgtacgg atacaaaatt agattgatgt
    11221 tttagtttgt aagaaaggat gaacgtggtc cagcaagaca tgcgtctata cgatctactt
    11281 gaccttttgt aaacatcacc atagtgtcat cgtcaacata atccatatag ttcataaaca
    11341 tatcgccatt cggtctatta tcacaagaaa ttgtaggaaa tgtaggcttt ccagtatttt
    11401 gatctgcttg atttggtgta tccgcaactc gatcagtgcc actgcatgca ccatcatcat
    11461 cgccaccaat atgaaataag ttaagccaat gtccaatttc atgagtagct gttcgaccta
    11521 aatggaaagg ctctgtcgca gtacctgtag tgccaaaata gttgtatcct ataactacac
    11581 catctgtttt agatggacca ccaggaaatt gggcgtatcc taaaagtcca tcctctaact
    11641 tacaaaccca cagatttaag tatttatcag ctggccatgc atttactcca cctgtacttt
    11701 gtgatttaac gggatcatct aaatatgaaa aagattgctt agttgtatgg gtacgagtga
    11761 taccatttgt ttgatttccg ttaggatctt ttgtagcaag aaagaattca actctagcgt
    11821 cacttacaga atctttaaag acagaaggca cgtttgctat atcagggttc gctgcacgga
    11881 aatcaagatt taaaacatca atttgactat gaacttgttc ctcagagata ttttgttcct
    11941 cagtattcca tacaacatga actacaacag gaattttgac aacgggtctt tcttcttgta
    12001 cacgtgcgct attgaggtat tcattcgtca tactttctat ttcaacacgt gcatttttat
    12061 attgttcgga ctcttgtagt agtttatggt gaagatctat agttgcgcat ctacgcacag
    12121 taggtgtaat tgttttttct gatgagttag tatgatttgt catagtaatt tctcctttag
    12181 attgtattta aatttttagt aattatatac ggtaatttag
//
