LOCUS       NZ_CP011151            10324 bp    DNA     circular CON 28-JAN-2024
DEFINITION  Bacillus cereus strain CMCC P0021 chromosome, complete genome.
ACCESSION   NZ_CP011151
VERSION     NZ_CP011151.1
KEYWORDS    RefSeq.
SOURCE      Bacillus cereus
  ORGANISM  Bacillus cereus
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus;
            Bacillus cereus group.
REFERENCE   1  (bases 1 to 5521782)
  AUTHORS   Wang,Y., Tian,W., Zhu,L., Wang,B., Wang,H. and Zeng,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-APR-2015) Institute for Biological Product Control,
            National Institutes for Food and Drug Control, No. 2, Tiantan Xili,
            Dongcheng District, Beijing 100050, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: RS_HGAP_Assembly v. V2
            Genome Coverage                   :: 35.03
            Sequencing Technology             :: Pacific Biosciences
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/28/2024 08:42:11
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,142
            CDSs (total)                      :: 5,983
            Genes (coding)                    :: 5,793
            CDSs (with protein)               :: 5,793
            Genes (RNA)                       :: 159
            rRNAs                             :: 16, 16, 16 (5S, 16S, 23S)
            complete rRNAs                    :: 16, 16, 16 (5S, 16S, 23S)
            tRNAs                             :: 106
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 190
            CDSs (without protein)            :: 190
            Pseudo Genes (ambiguous residues) :: 0 of 190
            Pseudo Genes (frameshifted)       :: 77 of 190
            Pseudo Genes (incomplete)         :: 106 of 190
            Pseudo Genes (internal stop)      :: 68 of 190
            Pseudo Genes (multiple problems)  :: 50 of 190
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 15:21:29
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 2646262
            Orig. end                         :: 2656586
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP011151.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10324
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="8"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10324
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10324
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:5324](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5324
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     misc_feature    338..352
                     /note="TFBS match to OsdR, Development and stress
                     management regulator, confidence: weak, score: 17.35"
                     /tool="antismash"
     gene            428..1876
                     /locus_tag="WR47_RS12950"
                     /old_locus_tag="WR47_13300"
     CDS             428..1876
                     /GO_function="GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /NRPS_PKS="Domain: Aminotran_1_2 (145-469). E-value:
                     5.7e-30. Score: 96.6. Matches aSDomain:
                     nrpspksdomains_WR47_RS12950_Aminotran_1_2.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_1_2"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1019: aminotransferase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016108507.1"
                     /locus_tag="WR47_RS12950"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="WR47_13300"
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="WP_000471611.1"
                     /sec_met_domain="Aminotran_1_2 (E-value: 5.6e-28, bitscore:
                     96.5, seeds: 48, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MFKDFKVVKDRPVYIQLKDYLKKMIMKGHLLGDQKIPSTRELSEL
                     LSVSRNTVLAAYADLEQEGLIYAVKGKGNFVAKVDISNTSSVEIDWKNKLNTVTALADE
                     LDLMKHGVRWEKGMIVFNSIAPDEKLFDVENFKRAFLTRMSIEGDIVLNYGYAKGYRPL
                     MNYLLHYMEMKGVDISNKDILITNGFTEGLDIVLSSLSKKSGRVICENPTHHAALKLFR
                     LHGLEVHGIDMHEDGIDTNEVEKSLREKDFDFAYLIPSYHNPTGIVTSSEKRTELMRLF
                     SKYNVPIIEDGFNEELRYSGSHLAPLLTFAGAGNNVIYISSFSKVLFPGLRVGWIIADK
                     ELIHYLESVKRARTIHTSTLDQAVLFQYLHEGYFEKYLKKARSVYKKKYELAVGACNQY
                     IPFKRMTGDGGLHLFIELEEHMNARTLLQKCYEKGVTFSPGDVFYSDGEGANTFRLGFS
                     RLKEEEIVRGIKIIGDTLKNENWS"
     aSDomain        863..1834
                     /aSDomain="Aminotran_1_2"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_WR47_RS12950_Aminotran_1_2.1"
                     /evalue="5.70E-30"
                     /label="WR47_RS12950_Aminotran_1_2.1"
                     /locus_tag="WR47_RS12950"
                     /protein_end="469"
                     /protein_start="145"
                     /score="96.6"
                     /tool="antismash"
                     /translation="IEGDIVLNYGYAKGYRPLMNYLLHYMEMKGVDISNKDILITNGFT
                     EGLDIVLSSLSKKSGRVICENPTHHAALKLFRLHGLEVHGIDMHEDGIDTNEVEKSLRE
                     KDFDFAYLIPSYHNPTGIVTSSEKRTELMRLFSKYNVPIIEDGFNEELRYSGSHLAPLL
                     TFAGAGNNVIYISSFSKVLFPGLRVGWIIADKELIHYLESVKRARTIHTSTLDQAVLFQ
                     YLHEGYFEKYLKKARSVYKKKYELAVGACNQYIPFKRMTGDGGLHLFIELEEHMNARTL
                     LQKCYEKGVTFSPGDVFYSDGEGANTFRLGFSRLKEEEIVRGI"
     gene            1881..2795
                     /locus_tag="WR47_RS12955"
                     /old_locus_tag="WR47_13305"
     CDS             1881..2795
                     /EC_number="6.3.2.4"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA]"
                     /GO_function="GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /GO_process="GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Dala_Dala_lig_N"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) ATP-grasp_3"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) RimK"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Dala_Dala_lig_C"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) ATP-grasp"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002038752.1"
                     /locus_tag="WR47_RS12955"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="WR47_13305"
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="WP_063549318.1"
                     /sec_met_domain="Dala_Dala_lig_N (E-value: 8.6e-22,
                     bitscore: 76.0, seeds: 508, tool: rule-based-clusters)"
                     /sec_met_domain="ATP-grasp_3 (E-value: 1.1e-13, bitscore:
                     49.9, seeds: 15, tool: rule-based-clusters)"
                     /sec_met_domain="RimK (E-value: 1.2e-08, bitscore: 33.1,
                     seeds: 4, tool: rule-based-clusters)"
                     /sec_met_domain="Dala_Dala_lig_C (E-value: 6.8e-53,
                     bitscore: 177.5, seeds: 12, tool: rule-based-clusters)"
                     /sec_met_domain="ATP-grasp (E-value: 1.6e-05, bitscore:
                     22.9, seeds: 20, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKIGVIMGGVSSEKQVSIMTGNEMIAHLDKNKYEIVPITLNEKMD
                     LIEKAKDIDFALLALHGKYGEDGTVQGTLESLGIPYSGSNMLSSSICMDKNISKKILRY
                     EGVETPDWIELTKMEDLNFDELDKLGFPLVVKPNSGGSSVGVKIVYNKNELISMLETVF
                     EWDSEVVIEKYIKGDEITCSILDGKQLPIVSIRHAAEFFDYNAKYDDTSTVEEVIELPA
                     EINERVNKASLACYKALKCSVYARVDMMVKDGIPYVMEINTLPGMTQSSLLPKSAEAAG
                     ISYSKLLDMIIETSLKVRNEEGF"
     misc_feature    complement(2818..2830)
                     /note="TFBS match to MatP, Cell cycle regulator,
                     confidence: weak, score: 19.27"
                     /tool="antismash"
     regulatory      2869..2974
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /regulatory_class="riboswitch"
     gene            3102..3791
                     /gene="tenA"
                     /locus_tag="WR47_RS12960"
                     /old_locus_tag="WR47_13310"
     CDS             3102..3791
                     /GO_function="GO:0050334 - thiaminase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="tenA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000144490.1"
                     /locus_tag="WR47_RS12960"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="WR47_13310"
                     /product="thiaminase II"
                     /protein_id="WP_000144507.1"
                     /transl_table=11
                     /translation="MTFSQSLRKEVDSIWEASFNHPFVKKLGEGTLDLASFRYYVLQDS
                     YYLSHFARVQTLGAAKALELETTARMAHHAQNTYEAELSLHENFAKKLGITKEEKDNFI
                     PAPTAYAYTSHMYRAAYEGHLGDIIAAILPCYWLYYEIGEHLKECQPEEPIYNEWISAY
                     GSDWFRTLVEEQITRLDTIAENVTEADRKRMKQHFIISSQYEYSFWEMAYTLEKWPVDK
                     DVKDVIG"
     gene            4189..4452
                     /locus_tag="WR47_RS12965"
                     /old_locus_tag="WR47_13315"
     CDS             4189..4452
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002101034.1"
                     /locus_tag="WR47_RS12965"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="WR47_13315"
                     /product="DUF3937 domain-containing protein"
                     /protein_id="WP_002082716.1"
                     /transl_table=11
                     /translation="MNFERCYMFTNKKLIRFGLSLFVFLGIINFTISYFQTYLETAADI
                     KWVIPEIWKTILLDVPQGILVLLGAIALYDFTKEASQKDASI"
     gene            5001..5324
                     /locus_tag="WR47_RS12970"
                     /old_locus_tag="WR47_13320"
     CDS             5001..5324
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: TIGR03601"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001071363.1"
                     /locus_tag="WR47_RS12970"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="WR47_13320"
                     /product="heterocycloanthracin/sonorensin family
                     bacteriocin"
                     /protein_id="WP_063547374.1"
                     /sec_met_domain="TIGR03601 (E-value: 1.7e-40, bitscore:
                     135.6, seeds: 6, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNQFQQELQALSLNDYRSGNIVYWDQQNQYPYYYIEDAARRCGGC
                     GRCGGCGGGRCGGFRCIGCFGCFGCGGCGGCGGCGGCGGCSNCFDGFSGTTDTTGTIIT
                     YEY"
     gene            <5474..5610
                     /locus_tag="WR47_RS32195"
                     /pseudo=""
     CDS             <5474..5610
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016077325.1"
                     /locus_tag="WR47_RS32195"
                     /note="frameshifted; incomplete; partial in the middle of a
                     contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /product="site-specific integrase"
                     /pseudo=""
                     /transl_table=11
                     /translation="YMYVPTKNIAIIIMDIFNHSSERVTIRYIGVIKR"
     misc_feature    5702..5719
                     /note="TFBS match to ArcA, Repressor of carbon oxidation,
                     confidence: medium, score: 19.24"
                     /tool="antismash"
     misc_feature    complement(5770..5788)
                     /note="TFBS match to LexA_variant_3, Repressor of DNA
                     damage response, confidence: medium, score: 24.87"
                     /tool="antismash"
     gene            5820..6305
                     /locus_tag="WR47_RS12975"
                     /old_locus_tag="WR47_13325"
     CDS             5820..6305
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016124614.1"
                     /locus_tag="WR47_RS12975"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="WR47_13325"
                     /product="hypothetical protein"
                     /protein_id="WP_002041181.1"
                     /transl_table=11
                     /translation="MNMIELKSIIHSYKLKRKIAKDLYGKRDELTLLLNEFNKMKCTVT
                     SEKKKNNILSRLQLIYQNMKLDKQYPLSVAFDSKLLERLEKEPLHTIEDGVTCLHLMLD
                     VNYEKIKQYGSSTSRSFVPLSQSSICLADCICLTGVVVGLLGAISFGGLLLSICSIT"
     misc_feature    6612..6625
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: medium, score: 23.3"
                     /tool="antismash"
     gene            6617..7288
                     /locus_tag="WR47_RS12980"
                     /old_locus_tag="WR47_13330"
     CDS             6617..7288
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016124615.1"
                     /locus_tag="WR47_RS12980"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="WR47_13330"
                     /product="DUF3962 domain-containing protein"
                     /protein_id="WP_000736204.1"
                     /transl_table=11
                     /translation="MKKLRLLTFENIVEPLLNEKVSFIYFPIEWLDIVEIHYKTFLLTS
                     KLKRLNERLYDMFSDILFIQHNPYVLNENTPWIVSKEPIRQEQLDYIFQSWYEIIHDWK
                     PNRLVEPPKYEWQSDLISNLPVLHDNETYSKWVPALISHIFCERPIYLENTNEEEIYFS
                     PLRSQNICEAMSGPIKDEKTQDFFSYVYRFECITRGGENAPLLNISIGIRRFYQEYKMI
                     G"
     gene            complement(7357..8466)
                     /locus_tag="WR47_RS12985"
                     /old_locus_tag="WR47_13335"
     CDS             complement(7357..8466)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /GO_process="GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="other (smcogs) SMCOG1077: phage integrase
                     family protein"
                     /gene_kind="other"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000675870.1"
                     /locus_tag="WR47_RS12985"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="WR47_13335"
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="WP_063547375.1"
                     /transl_table=11
                     /translation="MKGHIRKRGNKYCIVIDIGPDPETGKRRQKWFSGYKTKKEAQADV
                     AKKITELNEGTFIEPSKVTLKDYLNHWLEIKSMSIEKSTFAGYKAFINQHVIPSIGMVA
                     LHQLNVIHIQKCYKTAMDKGIASNSILLMHRILKSALNLAVKQNIMSRNPADFAEIPKK
                     EKSPIQTWTEEEVKKFLAHSQESRYHIGYLLAITTGMRLGEVLGLRWQDIDFEKHTVTI
                     NQTSGHDNKIKKTAKTNSSKRTIPVPNETIAALKKHKILINKEKLRFGSAYLDQDLINC
                     NEFGRIIKRAPFRKGFIRATHKVGIKEIKFHDLRHTHATILFKQGVNPKIISERLGHTD
                     ISMTLSIYSHVLPNMQEEAVKNFGKSIFG"
     gene            8698..9171
                     /locus_tag="WR47_RS12990"
                     /old_locus_tag="WR47_13340"
     CDS             8698..9171
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000732892.1"
                     /locus_tag="WR47_RS12990"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="WR47_13340"
                     /product="hypothetical protein"
                     /protein_id="WP_063547376.1"
                     /transl_table=11
                     /translation="MKKLFTVFFALVLAFTVIGTTPAMAAGDGNAQVYYNNEFNITNYV
                     FSKKPLLNSPSDPNPVGYVAWQYVTVKQAWFLIDTSLGPKWVGYNEDNGVLLWSRFDAA
                     HAKLFISEHRVGLHNDPSDPRVVGYVANQHVTVKQAWYLINTSLGQKWIGYNM"
     gene            9405..9866
                     /locus_tag="WR47_RS12995"
                     /old_locus_tag="WR47_13345"
     CDS             9405..9866
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000734558.1"
                     /locus_tag="WR47_RS12995"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="WR47_13345"
                     /product="hypothetical protein"
                     /protein_id="WP_000734558.1"
                     /transl_table=11
                     /translation="MKKLLAVFFAIALAFTAIGSTPAMAAGNSDARIISYVGVLQNYIY
                     GKKPLFNSPSDPTIVGYVANQYVTIKQPWFIVNTSLGEKWMGFNQWESSLWRDFDAGAG
                     RLYVSGKVAIRNDPGSQPVGYVANQNVTVKQAWYLINTSLGQKWIGYNL"
ORIGIN
        1 gcttaatgga aacagagaaa acacttactg atatacatat aaaaggaatc gagcatgtta
       61 caaaacagca aatacaaaat gctaagaaac aacataaaat tattaaatta atagcatcaa
      121 tatataagga tgagggtggc aatgtgaatt taaatgttga accgtgcgaa atagaaaaag
      181 atcatccgtt agcaaaagta aatgggagcg aaaaaggaat tacgttcttt acagatacga
      241 tgggacaagt tactacaatt ggtggggctt ctaatccacg aggcgctgca gctgcagctt
      301 taaaagatgt aattaactta tatcgaaaag atttgtaaga ccgtaccgcc ctttataaaa
      361 aatacatatg tcgtggacct tttgagaatg aaataaatat gcaaaaatga ttgcaggggg
      421 taaggaattg tttaaggatt ttaaagttgt taaagatcgt cccgtttata ttcaattaaa
      481 agactatttg aaaaagatga ttatgaaagg gcatttgctc ggggatcaaa aaatcccatc
      541 aacaagggaa ctaagtgaat tactaagtgt gagtagaaac actgtactcg ctgcttatgc
      601 agatttagag caagaaggac tcatttatgc agttaaagga aaagggaact ttgttgcgaa
      661 ggtggatata tctaacactt cgtctgttga aatagattgg aaaaataaac ttaatacagt
      721 gaccgcatta gcggacgaat tagatttaat gaaacatggt gttcgttggg aaaagggaat
      781 gattgttttt aatagtatag ccccggatga gaagctattt gatgttgaaa atttcaaaag
      841 agcttttctt actcgtatgt ccattgaagg ggacatcgta ttgaactacg gatatgcaaa
      901 gggatataga ccgttaatga attatttact tcattacatg gaaatgaaag gtgtagacat
      961 ttcgaataaa gatattctaa ttacaaatgg atttacagaa gggttggata ttgtactctc
     1021 ttctttatca aaaaaatccg ggcgtgtcat ttgtgagaat ccaactcacc acgctgcact
     1081 taagcttttc cgtttacatg ggcttgaagt tcatgggatt gatatgcatg aggatggtat
     1141 tgatacgaat gaagttgaaa aaagtttgcg tgaaaaagat tttgattttg cttatttaat
     1201 tccttcttat cataatccaa ccggtattgt tacgagttca gagaaaagaa cggaattgat
     1261 gaggttattc tcaaaataca acgttcctat tatagaggac ggatttaatg aagaattacg
     1321 ttattcaggt tcacatttag ctccattatt aacttttgct ggtgctggca ataatgtgat
     1381 ttatattagt agcttttcaa aggtactttt ccctggttta cgtgtagggt ggattattgc
     1441 agataaagag ctgattcatt atttggaaag cgtaaaaaga gcaagaacga ttcacacatc
     1501 tacgctagat caagctgttc tgtttcaata tttacatgag ggatattttg aaaaatattt
     1561 aaaaaaggca agatctgttt ataagaaaaa atatgagtta gctgtcgggg catgtaacca
     1621 gtacattcct ttcaaaagaa tgactggaga tggtggactg catttattta tagagctaga
     1681 agagcatatg aacgcccgca cacttttaca aaagtgttat gagaaaggcg ttacgttttc
     1741 acctggagat gttttttact ctgatggaga aggagcgaac actttccgat tagggttttc
     1801 gcgcttgaaa gaagaagaaa tagttcgggg aattaaaata attggtgata cattaaaaaa
     1861 tgaaaattgg agttgagaaa atgaaaatcg gtgttattat gggcggggta tcgtctgaaa
     1921 aacaagtttc aattatgacc gggaatgaaa tgattgctca tttagataaa aataagtatg
     1981 aaatagtccc aattacatta aatgaaaaaa tggatttaat tgaaaaagcg aaagacattg
     2041 actttgcatt gctcgcatta cacggaaaat acggagaaga tgggacagtt caaggaacac
     2101 tggagagttt agggattcct tatagcggaa gtaatatgtt atctagtagt atatgtatgg
     2161 ataaaaatat ttcgaaaaag attttacgtt atgaaggtgt agaaacacca gattggattg
     2221 aacttacaaa aatggaggat ctaaattttg atgagttaga taagttagga tttccattag
     2281 tggtaaaacc aaactccggc ggatcgagtg tcggggtgaa aatcgtttac aataaaaatg
     2341 aattgatctc aatgctagaa actgtattcg aatgggattc tgaagtagta attgagaagt
     2401 atataaaagg tgatgaaatt acatgttcta ttctggatgg aaaacagtta cctatagttt
     2461 caattcgaca tgcagccgag ttctttgatt acaatgcaaa atatgatgat actagtacag
     2521 ttgaggaagt tatcgaactt ccagcagaaa ttaatgaacg tgtaaataaa gcttcactgg
     2581 cttgttataa agcattaaaa tgtagtgttt atgcaagagt tgatatgatg gtgaaggacg
     2641 gaattccata tgtaatggaa attaatacat taccagggat gacacaatca agcctattgc
     2701 ccaaaagtgc agaagcagcg ggaattagtt atagcaaact attagatatg attattgaaa
     2761 catcattaaa agttagaaat gaagaagggt tttaaggaga ttatataact aattcttttg
     2821 acagtttcac gaagtatgct atgatggata cattataaaa acaaaatatt tatccactag
     2881 gggggccttt tataggctga gatcaaatgt gattttgaga ctcttagtac ctgatctggt
     2941 taatgccagc gtagggaagt gggaaagacg ttgctattta atgagtaaat aaatactgtc
     3001 aatttcactt tctttacgcc tgtaaagaaa gtttttttat tttatagctt taataattgt
     3061 ggataaatac ttatatttta tacattattg gagggattta aatgactttt tcacaatcat
     3121 tacgtaaaga agtagattca atttgggaag cgagttttaa ccatcctttt gtaaaaaaac
     3181 ttggtgaagg aacattagat ttagctagtt ttcgctatta tgtgcttcaa gattcatatt
     3241 atttgagtca ttttgctaga gtacaaactt taggagctgc aaaagctctt gaattagaga
     3301 cgacagctcg tatggcacat catgctcaaa atacatatga ggccgagtta tctttacatg
     3361 agaattttgc taaaaagcta gggataacaa aagaagaaaa agataatttt attcctgcgc
     3421 caactgcata tgcgtatact tcacatatgt atcgtgctgc gtatgaaggg catttaggag
     3481 atatcattgc agcaatttta ccttgctatt ggttgtatta tgaaattggc gaacacttaa
     3541 aagagtgcca gccggaagag ccaatttata acgaatggat ttctgcttat ggatctgatt
     3601 ggttccgtac gttagttgaa gaacaaatta cacgtttaga tactatcgct gaaaatgtaa
     3661 cagaggcaga ccgcaaacgt atgaaacagc attttattat tagcagtcaa tatgaatatt
     3721 cattttggga aatggcttat acattagaaa agtggccagt ggataaagac gtaaaagacg
     3781 taattggata aggaatagaa gtaatgttat agataggtat ggataatatc tatctatata
     3841 ttttttcttg tcaaatttgc gtaaagtcat tagtgctacg gatatggcga aatggaatat
     3901 cgataattta gctcatttac ttgtctacgc attaactgga taaacttctg tcacatttat
     3961 tttacaatca aagaacgtaa atgttggtgg tatgaagtta aataagaaaa gaaacccgag
     4021 ctgttagctg taacggagtt gttttttctt tgcaggaatt tctaaattat catggagtgt
     4081 tgttaatacg atatgcaaat atgcatatcg tatttttttg agttctttat gtgttaaaat
     4141 tagatataaa gatatggaaa tataaagaag caaaagcagg aatatccaat gaattttgag
     4201 aggtgttata tgtttacaaa taagaaatta attcgatttg gtttatcgtt gtttgtattt
     4261 ttagggataa ttaattttac aatcagctat ttccaaacat atcttgaaac agcagcagat
     4321 attaaatggg taattccaga aatttggaaa actattttac tagatgttcc tcaaggtata
     4381 cttgttcttt taggcgcaat tgctttatat gatttcacaa aagaggcatc acaaaaagac
     4441 gcatcaatct aagtgtgtct tttttctatt aggaatttca tagagagttt tgctggagaa
     4501 atagaatgaa aagccctaat aagggctttt ttctttgtag gaatttctta tccgtcatag
     4561 aaaccagtta cttggaggtg ttatagcgaa aggcatttac agtacagaaa aatctaagcg
     4621 gcagacggaa atatatagga tatgacaaca taattattga gaaattaatt gaatttagta
     4681 ggtatgaaga tatgacgcta gaattcatat tttttaattt ttatttatcc cgcattaacg
     4741 ggctgttcgt aaaagcctga ttggtgaggg ctaataatca gcgggggatg aactcccccc
     4801 actgattaaa gtttcacttt ataaaaatga gcccatagca aaaataggta tatgaacaag
     4861 gtgcatcatt tagtgataag tcatactttg ttggtgtacg tattgaactt agtttatagc
     4921 actattttta cttagatgta acgaacagaa atatagggaa gcattaatga ggttgtattt
     4981 tataaacgga ggttatgaaa atgaatcagt ttcaacaaga actacaagcg ttaagtctta
     5041 atgattaccg gtctggaaat attgtctatt gggaccagca aaaccaatat ccatattact
     5101 atattgaaga tgctgctcgt cgctgtggcg gttgtggtcg ttgtggtggt tgtggtggag
     5161 gtcgttgtgg cggattccgt tgtattggtt gcttcggttg ttttggttgc ggaggttgtg
     5221 gaggttgtgg aggttgtgga ggttgtggtg gttgctctaa ctgttttgat ggttttagtg
     5281 gtactactga tactactggt actattataa cgtatgaata ttgattgcat ttatcctgct
     5341 atttgcaggg cagtaagacc ctaaaaaaat tcagcgggag caaagagaag ttaggtggcg
     5401 gactacccat aaaagcccaa ttggtgaggg ctaattaaag tttcacctta tcaattaaaa
     5461 aacattagtt ttatatatgt acgtgccgac aaagaatata gccattatta taatggatat
     5521 attcaatcat tcaagtgaaa gagtaacaat aagatatata ggtgtaatca agaggtgatg
     5581 gataaagcaa tgactagatt taaaatctaa tcattgcttt tttcttatgg ttttatcatc
     5641 aaatatctgt ctggtttagg gattgattcc ttctagctaa tgaattcatt acaactttgt
     5701 aaggttaatg taatgttaat cgatagtaaa tgaagatgct tgagtgtaat ttaaataaga
     5761 ttaaaaagta ctgtttatct ataaaataat cgggtaaata atatgtaatt aggaagtgta
     5821 tgaatatgat tgaactaaaa tctattatcc attcgtataa actaaagaga aaaatagcta
     5881 aagatttata cggaaaaaga gatgaattga cactgttatt aaatgaattt aataaaatga
     5941 aatgtacagt aacatctgaa aagaagaaaa acaatatatt atctcgtttg caactaattt
     6001 atcaaaatat gaaattagat aagcagtatc ctctatcagt tgcttttgat agtaaattat
     6061 tggagcgatt agaaaaagaa cctctacata ctattgagga tggtgtaaca tgtttacatc
     6121 taatgttaga tgtgaattat gagaaaataa aacaatatgg ttcgagtaca agtaggtcat
     6181 ttgttccatt atcgcagtct tctatttgtc ttgctgattg tatttgttta acaggagttg
     6241 tagtaggttt actaggagca atttcatttg ggggattact gttatctata tgttcaatta
     6301 cataaaggtt gatatttgta aaaagggcac cgaattaagg tgttcttttt tggttttttt
     6361 gtaaagatat tgtaaatgat aagtacaatg cattaagtgt aatgtataat tcgaatgtat
     6421 cgaaattcaa atataataga ggtgttatac atgataatta ctttgaattt ttaaaagagt
     6481 cggtttgata ataatttatg tagataaaca gtttttacgt ttcttttaaa tacttggttt
     6541 aaatggatta gattaagtaa ctattgttct agctttttga taatcaagac aactgaagaa
     6601 aggaaggaat cattttatga aaaaattaag gctgctaaca tttgaaaata tagtagaacc
     6661 ccttttaaat gaaaaggtat catttatata ctttcctatt gaatggctgg acatcgtaga
     6721 gatacattat aagacatttt tattaacgag taagttgaaa cgtttgaatg aaagattgta
     6781 tgatatgttt tcggatatat tgtttattca gcataacccg tacgtattaa atgaaaatac
     6841 accatggatt gtatcgaaag aacctattag acaagagcaa ctcgattata tttttcaaag
     6901 ttggtatgag attattcatg attggaaacc aaatagatta gtagaaccac caaaatatga
     6961 atggcaatcc gatttgattt ctaatttgcc agtactacat gataatgaaa cgtattctaa
     7021 gtgggtgccc gctttaatct cacatatttt ttgtgagcgt cctatatatt tagaaaatac
     7081 aaatgaagaa gaaatctatt tttcccctct tagatcacaa aatatttgtg aggcgatgtc
     7141 agggccgata aaagatgaaa aaacacaaga ttttttctcc tatgtatatc gattcgaatg
     7201 cataacccgc ggtggtgaga acgctccatt attaaatatt tcaataggga ttcggagatt
     7261 ttatcaagaa tataagatga taggttaaac aaaccttgat ataacaacgt ttgtttgacc
     7321 tatttttatg aattgcaaac attttgcaaa catacgttat ccaaagatgc ttttaccaaa
     7381 gtttttaacg gcttcttctt gcatattcgg taaaacatga gaatagatac ttaatgtcat
     7441 tgaaatatct gtatgaccta atctctcact gatgatttta gggttaactc cttgtttaaa
     7501 tagtatagtt gcatgtgtat gtcttaaatc atggaattta atttctttta tacctacttt
     7561 gtgtgtcgcc ctaatgaaac cttttctgaa aggtgctctt tttatgattc ttccaaactc
     7621 attacaattt attaaatctt gatctagata agcagaacca aaccttaatt tctctttatt
     7681 aattaaaatt ttatgttttt ttaaggcggc tattgtttca ttaggtacag gaattgtgcg
     7741 ttttgacgaa tttgtttttg cagttttttt gattttattg tcatgaccag atgtttgatt
     7801 tattgtaacg gtatgttttt caaaatcaat gtcctgccat cgtaacccta gaacttctcc
     7861 tagacgcata cctgtagtta ttgcaagtag atacccaatg tgatatcgtg attcttgtga
     7921 atgagctaaa aattttttta cttcttcctc tgtccaagtc tggatagggg atttttcttt
     7981 tttaggtatc tcagcaaaat ctgctggatt tcgagacata atattttgtt ttacggctag
     8041 gtttaaagcg ctctttaaaa ttctatgcat aagcagaata gaattgcttg caatcccttt
     8101 atccatcgca gtcttataac atttttgaat gtgtataaca tttaattgat ggagtgcaac
     8161 cattcctata ctaggtataa catgttggtt gataaacgcc ttatagccag caaaggtact
     8221 cttttctatg ctcatacttt taatttctag ccaatgattt agataatcct ttaacgtaac
     8281 tttagatggt tctataaaag ttccttcatt caactctgta attttctttg ccacatcagc
     8341 ctgtgcttct ttttttgtct tatatccaga aaaccatttc tgtcttcttt ttcctgtctc
     8401 tggatcagga ccgatatcaa taacaataca atacttattt cctctttttc gaatatgtcc
     8461 tttcacttaa aacactcctt catttgtttt gaatcatgtt gtataagtct agttgtaatt
     8521 ttgctgctat gaaaattaca tagttggaca tatcagcgat ggatacattt taccatatat
     8581 aagtaaattc agttatagga tatgattgta aaataatact aattttatct ttacaatata
     8641 cggaaaattc ggtaatatac ttgatggaat tataaatcaa ttcaaggggg aagaaagatg
     8701 aaaaagttat ttacagtatt ttttgcacta gtattggcat ttacagttat tggtactacg
     8761 ccagctatgg cagcgggaga cgggaatgca caagtttatt ataataatga attcaatata
     8821 actaattatg tgttttcaaa aaaaccttta ttaaatagtc caagtgatcc aaacccagta
     8881 ggttatgttg cttggcaata tgttacagta aaacaagcat ggtttttaat tgatacatct
     8941 ttagggccta agtgggtagg atataatgaa gataatggag tcttgctgtg gagtagattc
     9001 gatgcggctc atgcgaagct ttttattagt gaacatagag tgggattaca taatgatcca
     9061 agtgaccctc gtgtagtagg ttatgtagca aatcaacatg tcacagtaaa acaagcatgg
     9121 tatttaatta atacatcatt aggacaaaaa tggattggtt ataacatgta aaaggcgctc
     9181 attatgagtg cttttttatt tgtatatata agtctagttg taattttgct gctataaaaa
     9241 ttacatagtt ggacatatca gcgatggata tattttacca tatatgaaca aattcagtta
     9301 taggataaga ttgtaagata atactaattt tatctttaca atatacggaa aattctgtaa
     9361 tgtaattaat ggaattataa agcgattcaa ggggaggaag aaagatgaaa aagttattag
     9421 cagtattttt cgcaatagca ttagcattta cggctattgg ttctacacca gctatggcag
     9481 cagggaattc tgatgcaaga attatatctt atgtcggagt actacaaaac tatatttatg
     9541 gtaaaaagcc actattcaat agtccaagtg atccaacaat agtaggatat gtagctaatc
     9601 aatatgtaac aataaagcaa ccatggttta ttgttaatac atctttaggg gaaaaatgga
     9661 tgggattcaa tcaatgggaa tcttcactat ggcgtgattt tgatgcaggc gcgggacgtt
     9721 tatatgtatc aggaaaagtg gctatacgca atgatccggg ctctcaacca gtaggttatg
     9781 ttgctaatca aaacgttaca gtaaaacaag catggtattt aattaataca tcattaggac
     9841 aaaaatggat aggttataat ttgtaaaaaa gcgctcatta tgagtgtttt tttgtatata
     9901 taagtctagt tgtaattttg ctgctataaa aattacatag ttggacatat cagcgatgga
     9961 tacattttac catatatgag caaattcagt tatagaaagt gatttccatt acttgttaat
    10021 aagtagttag acacgataca atatttaagt attcaatagt agaaataaaa tagtagaatt
    10081 agtaatgtat atttaaaagt tatagagttt gaaggagagg gaattatggt tacaagtaag
    10141 gtgttaaaag aaatatatat ttcggaggat agtgatatat ataatgaact ttcaccagtt
    10201 gggaagttta gttttttatt tgatggatac atttttcgag gggaaagtag caaatataag
    10261 cttttgccaa gtgctttaag agaagatgaa cagagtttta ataagctttt cgaatttgct
    10321 ggtc
//
