LOCUS       NZ_KV411304            10957 bp    DNA     linear   CON 12-FEB-2024
DEFINITION  Nocardia terpenica strain IFM 0406 scaffold2, whole genome shotgun
            sequence.
ACCESSION   NZ_KV411304
VERSION     NZ_KV411304.1
KEYWORDS    WGS; RefSeq.
SOURCE      Nocardia terpenica
  ORGANISM  Nocardia terpenica
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Nocardiaceae; Nocardia.
REFERENCE   1  (bases 1 to 4638951)
  AUTHORS   Buchmann,A., Eitel,M., Koch,P., Schwarz,P.N., Stegmann,E.,
            Wohlleben,W., Wolanski,M., Krawiec,M., Zakrzewska-Czerwinska,J.,
            Mendez,C., Botas,A., Moris,F., Cortes,J. and Gross,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-APR-2016) Pharmaceutical Biology, University of
            Tuebingen, Auf der Morgenstelle 8, Tuebingen 72076, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 30-JUL-2014
            Assembly Method                   :: CLC NGS Cell v. 7.0.4
            Genome Coverage                   :: 118.0x
            Sequencing Technology             :: Illumina HiSeq; PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/12/2024 01:56:15
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,832
            CDSs (total)                      :: 8,760
            Genes (coding)                    :: 8,562
            CDSs (with protein)               :: 8,562
            Genes (RNA)                       :: 72
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 198
            CDSs (without protein)            :: 198
            Pseudo Genes (ambiguous residues) :: 0 of 198
            Pseudo Genes (frameshifted)       :: 40 of 198
            Pseudo Genes (incomplete)         :: 174 of 198
            Pseudo Genes (internal stop)      :: 9 of 198
            Pseudo Genes (multiple problems)  :: 23 of 198
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 16:51:21
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 3735012
            Orig. end                         :: 3745969
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            KV411304.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10957
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="butyrolactone"
                     /region_number="26"
                     /rules="AfsA"
                     /tool="antismash"
     cand_cluster    1..10957
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="AfsA"
                     /kind="single"
                     /product="butyrolactone"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10957
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5957](-)"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5957)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
     gene            464..1558
                     /locus_tag="AWN90_RS38040"
                     /old_locus_tag="AWN90_38060"
     CDS             464..1558
                     /GO_function="GO:0008171 - O-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1042: O-methyltransferase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019045794.1"
                     /locus_tag="AWN90_RS38040"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AWN90_38060"
                     /product="methyltransferase"
                     /protein_id="WP_067593296.1"
                     /transl_table=11
                     /translation="MALPDLVSRLPKLPPLPMIRAVELVRYGLAEAYRRLAPGPLALME
                     LLAAGWLTQAIHAAAALGVADALAERPRDATDLARAVGADVDALTRLLRLLTSYGIFTR
                     RRNGTYALTSAGRALCRDSPTSLRDAALFFGSGPHRRHWTHIVDAVRTGRPVGQALDGM
                     EFFDYLRTDRELGDLFDRAMTSIGTLTAESLFAAYDFTRFGTVVDVGGGRGALLTDILA
                     RAPRSRGILFDLPEVVAEADPHRSDRIELRGGSFFDAVPAGGDAYILKHVVHDWPDASA
                     ERILRTVRAAMTADARLILVEIVLPEDNRPHPGKYIDLEMLVNSGGRERTEADYRSLLA
                     RAGLAVTRVVPTASPESVLEARPA"
     gene            complement(1562..2017)
                     /locus_tag="AWN90_RS38045"
                     /old_locus_tag="AWN90_38065"
     CDS             complement(1562..2017)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019046971.1"
                     /locus_tag="AWN90_RS38045"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AWN90_38065"
                     /product="pyridoxamine 5'-phosphate oxidase family protein"
                     /protein_id="WP_067593299.1"
                     /transl_table=11
                     /translation="MGARDAAGRRRAENTPAHVSRDLTTTESLRLLAGVRFGRIVYSRY
                     ALPTIRPVNHLVDGETIIVHAERAAGLAPERQVVAYEADTLDEDTRRGWCVIVTGTSDR
                     VTDPAEIDRYRGQLCAWLPGPRDHFVRIEPDIITGIEFVDPDDPRLA"
     gene            complement(2465..2974)
                     /locus_tag="AWN90_RS38050"
                     /old_locus_tag="AWN90_38070"
     CDS             complement(2465..2974)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014988092.1"
                     /locus_tag="AWN90_RS38050"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AWN90_38070"
                     /product="hypothetical protein"
                     /protein_id="WP_067593302.1"
                     /transl_table=11
                     /translation="MYVPQPDGRIVAAGPAPDDSARTRLPDWHELLAAFCGHIGDQPDD
                     HPITRWARALAELHLARREHPLHVAEIDGRRNELVAHIDDWVAAHTARRPRSQSLGAVV
                     DGMAAAQVRATHLLRSVDDVTDDRVHSAWFQLASMADGWTDLVEQVLGVAARPLARPLR
                     SRAAGA"
     misc_feature    complement(2900..2902)
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            complement(3198..4133)
                     /locus_tag="AWN90_RS38055"
                     /old_locus_tag="AWN90_38075"
     CDS             complement(3198..4133)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014349694.1"
                     /locus_tag="AWN90_RS38055"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AWN90_38075"
                     /product="hypothetical protein"
                     /protein_id="WP_067596181.1"
                     /transl_table=11
                     /translation="MTESITQRRTGIAVAMGRLARFTATRYKPYYLLYAVVWTLAVEAT
                     AALVSHPQVGWRPGGATVVRVVVVAGVLLYLRMVDEQKDLGYDRIHYPDRPLVTGAITT
                     RELRIAMTVLAAAAIGASLALSVWSGVWITIVLGYGLVLWGLERASAAIRDTLLLNLAV
                     TYPVQVWLTVYVVVSAIGTGQVQSRWVTAGVAVVFAGAFLGFEFARKTTSTARPGEWFY
                     SNILGATGSAAAVVSCSVLAVGCDLMLVQPWRYPITHAVVGWIAVVALVFPLWGTLRFR
                     RQNRDYPVAAAVLFILTLYATLITQALVLP"
     misc_feature    complement(3813..3815)
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            complement(4450..5004)
                     /locus_tag="AWN90_RS38060"
                     /old_locus_tag="AWN90_38080"
     CDS             complement(4450..5004)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF00881"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014982638.1"
                     /locus_tag="AWN90_RS38060"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AWN90_38080"
                     /product="nitroreductase family protein"
                     /protein_id="WP_067593305.1"
                     /sec_met_domain="PF00881 (E-value: 1e-20, bitscore: 72.5,
                     seeds: 78, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTDLDLLTTTRAFRRRLDPTAPISYRDIQDCLDIAVHAPSGSNRQ
                     PWHFVLIDDPHTRHTIADYYRQGFAHNLSGRTPRPDQLRDLDSARHLADHLHTIPLLIL
                     VCTHGRPPATAAHLASFYASIYPTVWNLQLALHAHGYGTCLTTAHLTHEHDTATLLNIP
                     YDNITQRRPDRGGREDGHGDA"
     gene            complement(5001..5957)
                     /locus_tag="AWN90_RS38065"
                     /old_locus_tag="AWN90_38085"
     CDS             complement(5001..5957)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     butyrolactone: AfsA"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014349692.1"
                     /locus_tag="AWN90_RS38065"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AWN90_38085"
                     /product="ScbA/BarX family gamma-butyrolactone biosynthesis
                     protein"
                     /protein_id="WP_067593308.1"
                     /sec_met_domain="AfsA (E-value: 1.9e-19, bitscore: 67.5,
                     seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTTDIARPSTATHIRTISRHLAHRCAVSEVFVTSLHANTEDSYTV
                     GAQLPRMHAYYGDHVGAAAVQYDPLLVMEAARQAAIALTHEYFGVPGDMAFVVRTFNGS
                     GADTAAWRIGPTPADLVMTVQITRRHQHHGRVHGVDMVLDIACADTPMMTVDGSFTWIT
                     PHQWEQLRDSARTGLGLGGFDGTTVSGERADPATVGRESRRNVVIGPLQPHSEGGTAAL
                     VADTGHPALFDHPLDHAPGSLLIEAARQTATALLGPNPPQLLCVASTFEKFVELDHPAA
                     CIAEIVQTDPVVIRCDIHQGDTTAARIDLEFAEVPEQ"
     gene            complement(5954..6859)
                     /locus_tag="AWN90_RS38070"
                     /old_locus_tag="AWN90_38090"
     CDS             complement(5954..6859)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014982636.1"
                     /locus_tag="AWN90_RS38070"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AWN90_38090"
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="WP_231951842.1"
                     /transl_table=11
                     /translation="MTNLRELLMPLTPQSAKVDAYVADTYVQEAVTQLESRGVDPAVFA
                     RKYSMLLLKPDAIVARAVDKTLVWLRDNGFRVVAVRAVPVGRHFVRALWYFAWNIASPE
                     RRRIADLLAAVSDALVLVIASDTDTMPTPVRLAAGKGATNPAKRHPGELRYLLGRHNYL
                     LNLVHSPDDPADVLREFAIYFDERTRTQILTEINTGRDRSALASELGDHLYALTPARSF
                     DRATALERILTEIGGAPPGFDPACDADCARLLYQAWAQDRPLDPWSVIVLGSHVLPMRT
                     GTQPQTLPPVTAHDWLKDRP"
     gene            complement(6856..7998)
                     /locus_tag="AWN90_RS38075"
                     /old_locus_tag="AWN90_38095"
     CDS             complement(6856..7998)
                     /NRPS_PKS="Domain: NAD_binding_4 (8-228). E-value: 1.1e-42.
                     Score: 137.8. Matches aSDomain:
                     nrpspksdomains_AWN90_RS38075_NAD_binding_4.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) RmlD_sub_bind"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) NAD_binding_4"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1010: NAD-dependent epimerase/dehydratase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014982635.1"
                     /locus_tag="AWN90_RS38075"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AWN90_38095"
                     /product="SDR family oxidoreductase"
                     /protein_id="WP_067593314.1"
                     /sec_met_domain="RmlD_sub_bind (E-value: 7.2e-18, bitscore:
                     62.7, seeds: 37, tool: rule-based-clusters)"
                     /sec_met_domain="NAD_binding_4 (E-value: 8.1e-41, bitscore:
                     137.8, seeds: 102, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTGTGAVLLTGATGLVGAEVAARLLAAGREVVAVRHTTSRILRND
                     GTVLDGVHAVPGDIRRPGLGLTPNEPGVVDDQRVGVIVHSAATTAFDATDTDYQELNVQ
                     ATAHAVELALAWGVPLVYVSTAYVCGMQGGTIEEAELEGGSGFGNRYEDSKFRAEHLVR
                     GAAGLRWVIVRPGIVTGATGTGRIRDFKNLYTVVKLIVEGKLRVLPGRYDATIALAPVD
                     HVADVITTATLNVDTASGRTFHALGRGGLSLREVSDVLAEYPSFRVATFVPESTFTAED
                     LDPLQREYFLRIGALYTSYFQRRPVFATANADALLGRSSPATGKDYLRLVLDYCLESGY
                     LGTPLPTVRDVLARYVQPPKGNGVAASRGTGDDRNLGGVL"
     aSDomain        complement(7315..7974)
                     /aSDomain="NAD_binding_4"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_AWN90_RS38075_NAD_binding_4.1"
                     /evalue="1.10E-42"
                     /label="AWN90_RS38075_NAD_binding_4.1"
                     /locus_tag="AWN90_RS38075"
                     /protein_end="228"
                     /protein_start="8"
                     /score="137.8"
                     /tool="antismash"
                     /translation="LTGATGLVGAEVAARLLAAGREVVAVRHTTSRILRNDGTVLDGVH
                     AVPGDIRRPGLGLTPNEPGVVDDQRVGVIVHSAATTAFDATDTDYQELNVQATAHAVEL
                     ALAWGVPLVYVSTAYVCGMQGGTIEEAELEGGSGFGNRYEDSKFRAEHLVRGAAGLRWV
                     IVRPGIVTGATGTGRIRDFKNLYTVVKLIVEGKLRVLPGRYDATIALAPVDHVADVI"
     gene            complement(7995..8261)
                     /locus_tag="AWN90_RS38080"
                     /old_locus_tag="AWN90_38100"
     CDS             complement(7995..8261)
                     /GO_function="GO:0000036 - acyl carrier activity [Evidence
                     IEA]"
                     /GO_process="GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /NRPS_PKS="Domain: PP-binding (10-57). E-value: 2.7e-10.
                     Score: 32.4. Matches aSDomain:
                     nrpspksdomains_AWN90_RS38080_PP-binding.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PP-binding"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014349689.1"
                     /locus_tag="AWN90_RS38080"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AWN90_38100"
                     /product="acyl carrier protein"
                     /protein_id="WP_067593317.1"
                     /sec_met_domain="PP-binding (E-value: 2.4e-09, bitscore:
                     35.6, seeds: 164, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTDIDHSTPARVRELIAAMAPDPGSAVTDDQQLVTDLGFDSLRLM
                     ELTLVLEQAFDLPRYRPDQLAGVHRVTDVIALIEQGLSERSQP"
     aSDomain        complement(8091..8231)
                     /aSDomain="PP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_AWN90_RS38080_PP-binding.1"
                     /evalue="2.70E-10"
                     /label="AWN90_RS38080_PP-binding.1"
                     /locus_tag="AWN90_RS38080"
                     /protein_end="57"
                     /protein_start="10"
                     /score="32.4"
                     /tool="antismash"
                     /translation="RVRELIAAMAPDPGSAVTDDQQLVTDLGFDSLRLMELTLVLEQAF
                     DL"
     misc_feature    10307..10321
                     /note="TFBS match to MexT, Global virulence regulator,
                     confidence: weak, score: 16.64"
                     /tool="antismash"
     misc_feature    complement(10760..10774)
                     /note="TFBS match to MexT, Global virulence regulator,
                     confidence: weak, score: 16.84"
                     /tool="antismash"
ORIGIN
        1 gctgctggag acgctgcgca tgtgggggcc gtgggtgagc acgaacaccg ccgccgggcc
       61 gatcaccctc ggcgacgtca cgctgcccgc gggatcggtg gtcgcgttca gcccgtacat
      121 gattcaccac gacccgcgct atttcaccgc cccggagaca ttcgaccccg accgctgggc
      181 ccccgggcgg gtcgaggaga tcgaacgggc cgcggtgctg ccgttctcgg tgggccccag
      241 gcactgtccc ggcaacaatt tcgcgctgct gaccatcacg ctgcagtcgg cggcgctgct
      301 gtcgcgctgg gaaccgatcc ccgatccggg ctaccgcgtc cggcccgcgg gccgcgactt
      361 cgtggcctca cccgcccgac tcccggtgcg gttgcggccg cgccgggcga tcaccccata
      421 ggctcggcac cgaccgccga ccatccgacc ggagggagca gccatggccc tacccgatct
      481 ggtatcccgt ctcccgaaac tgcctccgct gccgatgatc cgggccgtgg agctggtgcg
      541 ctacgggctg gcggaggcgt accggcggct cgcgcccggc ccgctggcgc tgatggagct
      601 gctggccgcg ggctggctga cccaggccat ccacgccgcc gccgcgctcg gcgtcgccga
      661 cgcactggcc gaacggccgc gcgacgcaac ggatctggcg cgcgcggtgg gcgccgacgt
      721 cgacgccctc acccgcctgc tgcggctgct gaccagctac ggcatcttca cccgccgccg
      781 caacggcacc tacgcgctga cctcggccgg gcgcgcgctg tgccgggact cgccgacctc
      841 gctgcgagat gccgcgctgt tcttcggttc cgggccgcat cgccgccact ggacccatat
      901 cgtcgacgcc gtccgcaccg ggcggccggt gggtcaggcg ctggacggga tggaattctt
      961 cgactatctg cgcaccgacc gggaattggg cgatctgttc gaccgcgcga tgaccagcat
     1021 cggcaccctc accgccgaat ccctgttcgc cgcctacgat ttcacccgat tcggcaccgt
     1081 cgtcgacgtc ggtggcggcc gcggggcgct gctgaccgac atcctcgcgc gcgccccgcg
     1141 ctcgcgcggc atcctgttcg atctgcccga ggtggtggcc gaggccgacc cgcaccgatc
     1201 cgatcggatc gagctgcgcg gcggatcgtt cttcgacgcc gtgcccgcgg gcggcgacgc
     1261 ctacatcctc aagcacgtcg tgcacgactg gcccgatgcg agcgccgagc ggatcctgcg
     1321 cacggtgcgc gccgccatga ccgccgacgc ccgcctgatt ctcgtggaaa tcgtgctgcc
     1381 cgaggacaat cggccgcatc cgggcaaata catcgatctg gagatgctgg tgaacagcgg
     1441 cgggcgcgag cgcaccgagg ccgactaccg gtcgctgctg gcgcgcgcgg gactggccgt
     1501 cacccgcgtg gtgcccacgg cctcaccgga atccgtgctc gaggcccgcc cggcgtagcg
     1561 gtcaggccaa ccgcggatcg tccgggtcga cgaattcgat gccggtgatg atatccggtt
     1621 cgatgcggac gaaatggtcg cgcgggccgg gcagccacgc gcacagctga ccccggtagc
     1681 ggtcgatctc cgccgggtcg gtgacccggt cggaggtgcc ggtgacgatg acgcaccagc
     1741 cgcgccgggt gtcctcgtcg agggtgtcgg cctcgtaggc gacgacctgc cgttccggcg
     1801 ccagcccggc cgcgcgctcg gcgtgcacga tgatcgtttc cccgtcgacg aggtgattca
     1861 ccggccggat ggtgggtagc gcgtagcggg agtagacgat ccggccgaac cgcacgcccg
     1921 cgagcaggcg tagcgattcg gtcgtcgtca gatcccgcga cacgtgcgcg ggggtgttct
     1981 cggcccggcg gcggcccgcg gcgtcgcgcg cgcccacccg gtgtgcgatt cccgacgaac
     2041 atggcgacgg cggccgcctc gcgaagtcgt cgactcgacc ggtcatcgtg ttgttagccc
     2101 caaaagtcgt ccattgtgat gcggtccgga cacataacga cgttcccgga accgggacgc
     2161 ccgaaacccg aaacgttttg tcgaccccgg acatcggcgg ttcgctgcgc gatatcgccg
     2221 acgtcaccgc gcacatgctg cccggtcacc cgctggttgc gagcatattg cgacgcggcg
     2281 gtgacgagat tccaccggac cgcgcgccac ccgtcatcga cgaaccgggg ccggtgaccg
     2341 ggcaggaacc gactccccca tttcgattcg cctgcgcgac aacggcgcgc cctcccccgg
     2401 tattcgaccc ccgtggccgc gggcaccacc cggccgtcac gccgacgccg gcggcgccga
     2461 ccgttcaggc acccgcggcc cgggagcgaa gcgggcgtgc gagcggccgt gcggccaccc
     2521 ccagcacctg ctcgaccaga tcggtccagc cgtcggccat cgacgccagc tggaaccagg
     2581 cactgtgcac tcgatcgtcg gtgacgtcgt cgacgctgcg cagcagatgg gtggcgcgga
     2641 cctgagccgc ggccatcccg tcgacgacgg cgcccagcga ctgcgagcgc gggcggcgcg
     2701 cggtatgggc ggccacccag tcgtctatgt gggcgacgag ttcgttacgg cgaccgtcga
     2761 tctcggccac gtgcaacgga tgttcacgac gcgccagatg cagttcggcg agggcgcgcg
     2821 cccagcgggt gatcggatga tcatcgggtt gatcgccgat atgcccgcag aaggctgcca
     2881 gcagttcgtg ccaatccggt aaccgggtcc gggcgctgtc gtccggggcc ggtcccgcag
     2941 cgactatcct gccgtccggc tgcggtacgt acatctcgag gtacctccaa agcgggctcg
     3001 aacgcacccg gcgccacacg cgcggacacc ggaccgcgag cccggtgcgg caagggcgga
     3061 ccgcaccgga tccggcgcgc cgcaatcttg cgaagggggc ttttcctcga gcggcgccgg
     3121 gtgcacctcc agaatgcgcg ccgaacccgg tccgcccact gccctttcgg tggccctccg
     3181 atgaccatcc gcaaagctca cggcagaacg agtgcttggg tgatgagtgt ggcgtacagg
     3241 gtgagtatga acagcacagc cgcggcaacg gggtaatccc ggttttgtcg ccggaatcgc
     3301 agcgtgcccc atagcggaaa caccaacgct accaccgcga tccaccccac caccgcgtgt
     3361 gtgatcgggt accgccacgg ctgcaccagc atcaggtcac accccaccgc cagcaccgag
     3421 cacgacacca ccgcggcggc gctgccggtc gccccgagaa tgttggagta gaaccactcc
     3481 cccggccggg ccgtgctggt ggttttgcgg gcgaattcga atcccagaaa cgctcccgcg
     3541 aatacgaccg cgactcctgc tgtgacccat cgggattgga cctgcccggt cccgatggcc
     3601 gacaccacca cgtagacggt gagccatacc tgcaccggat aggtgacggc cagattcagc
     3661 aacagcgtgt cccggatggc cgccgatgcc cgttccaggc cccacagcac cagcccgtac
     3721 ccgagcacga tggtgatcca tacccctgac cacaccgaca gtgccagcga cgccccgatc
     3781 gcggcggccg cgaggacggt catggcgatg cgtaactcgc gtgtggtgat ggccccggtg
     3841 accaacggtc gatccggata atgaatccgg tcgtatccca ggtctttctg ttcgtcgacc
     3901 atccgcagat acagcaacac tcctgccacc accacgaccc gcaccaccgt cgccccgccc
     3961 ggccgccacc cgacctgcgg gtgcgacacg agtgcggcgg tggcttcgac cgccagtgtc
     4021 cacacgaccg cgtacagcag gtagtacggc ttgtacctgg tggcggtgaa ccgcgccaga
     4081 cgacccatcg cgacggcgat accggtgcgg cgctgggtga tcgactcggt catgaccgag
     4141 tcgatcaccc agcgccgcac cggtatcgcc gtcgcgatgg gtcgtctggc gcggttcacc
     4201 gccaccaggt acaagccgta ctacctgctg tacgcggtcg tgtggacact ggcggtcgaa
     4261 gccaccgccg cactcgtgtc gcacccgcag gtcgggtggc ggccgggcgg ggcgacggtg
     4321 gtgcgggtcg tggtggtggc aggagtgttg ctgtatctgc ggatggtcga cgaacagaaa
     4381 gacctgggat acgaccggat tcattatccg gatcgaccgt tggtcaccgg ggccatcacc
     4441 acacgcgagt tacgcatcgc catgaccgtc ctcgcggccg ccgcgatcgg ggcgtcgctg
     4501 ggtgatgttg tcgtagggga tgttgaggag ggtggctgtg tcgtgttcgt gggtgaggtg
     4561 ggcggtggtg aggcaggtgc cgtagccgtg ggcgtgcagg gctagttgca ggttccagac
     4621 ggtggggtag atggaggcgt agaagctggc caggtgggcg gcggtggccg gggggcggcc
     4681 gtgggtgcag acgaggatga gtagggggat ggtgtggagg tggtcggcga ggtggcgggc
     4741 ggagtcgaga tcgcgcagtt ggtcggggcg gggggtgcgg ccggacaggt tgtgggcgaa
     4801 gccttggcgg tagtagtcgg cgatggtatg gcgggtgtgg gggtcgtcga tgaggacgaa
     4861 gtgccagggt tggcggttgg agccgctggg ggcgtgcacg gcgatatcga ggcagtcctg
     4921 gatgtctcgg taggagatgg gggcggtggg gtcgaggcgg cggcgaaacg cgcgggtggt
     4981 ggtcaacagg tcgagatcgg tcactgttcg ggtacctcgg cgaattccag gtcgatacgg
     5041 gcggcggtgg tgtcgccctg gtggatgtcg cagcggatca cgacaggatc ggtctggacg
     5101 atctcggcga tgcatgcggc ggggtggtcg agttccacga atttctcgaa tgtgcttgcg
     5161 acacagagta gttggggtgg gttcgggccc agcagggcgg tcgctgtctg gcgggcggct
     5221 tcgatgagca ggctgccggg ggcgtggtcg agggggtggt cgaacagggc gggatggccg
     5281 gtgtcggcga ccaaagccgc tgtgccgcct tcggagtggg gttggagcgg gccgatcacg
     5341 acattgcggc ggctctcccg gcccaccgtc gccgggtcgg cgcgctcacc ggacacggtc
     5401 gtgccgtcga acccacccag gcccagaccg gtgcgggcgc tgtcgcgtag ttgttcccat
     5461 tggtgggggg tgatccaggt gaaggagccg tcgacggtca tcatcggcgt atcggcgcag
     5521 gcgatgtcga ggaccatgtc cacgccgtgg acgcggccgt ggtgctggtg gcggcgtgtg
     5581 atctgcacgg tcatgaccag atcggcgggg gtgggtccga ttcgccaggc ggcggtgtct
     5641 gcgccgctgc cgttgaaggt gcgcaccacg aacgccatat cgcctggcac gccgaagtat
     5701 tcgtgcgtca acgcgatcgc ggcctgacgg gccgcctcca tcaccagcag cgggtcatac
     5761 tgcacggcag ccgctcccac gtgatcaccg tagtaggcgt gcatgcgggg tagctgcgcg
     5821 ccgacggtgt agctgtcttc ggtgtttgcg tgtagggagg tgacgaagac ttccgagacc
     5881 gcgcagcggt gggcgaggtg gcgggagatc gttcgtatgt gggtggcggt ggatggtcgg
     5941 gcgatgtcgg tggtcatggg cgatccttca gccagtcgtg ggcggtgacc gggggcagcg
     6001 tctggggctg ggtcccggtg cgcatgggta ggacgtggga gccgagcacg atcaccgacc
     6061 acggatccag cggccgatcc tgcgcccacg cctggtacag caaccgcgca cagtcggcat
     6121 cgcaggcggg gtcgaaaccc ggtggcgcgc cgccgatttc ggtgaggatc cgctccaacg
     6181 ccgtcgcccg gtcgaaactg cgggcggggg tgagggcata caggtggtca ccgagttcac
     6241 tggccagcgc cgaccggtcg cggccggtat tgatctcggt caggatctgg gtgcgggtgc
     6301 gttcgtcgaa atagatcgcg aactcccgca acacatccgc cggatcatcc ggggaatgca
     6361 ccagattcaa cagataattg tggcggccca gcagataccg caactccccc ggatgacgct
     6421 tggccggatt cgtggcaccc ttgcccgcgg ccaaccgcac cggggtgggc atggtgtcgg
     6481 tgtcgctggc gatgaccaac accagagcat cggagaccgc ggcgaggagg tcggcgatgc
     6541 ggcggcgttc gggagaggcg atattccagg cgaaatacca caacgcacgc acgaaatgcc
     6601 gccccaccgg caccgcccgc accgccacca cccgaaaccc attatcccgc aaccacacca
     6661 acgtcttatc caccgcacgc gcgacgatcg catccggctt caacaacaac atcgaatact
     6721 tacgcgcgaa aacagcagga tcaacgcccc gggattccag ctgtgtcacc gcctcctgaa
     6781 cataggtgtc ggcgacatag gcgtccacct tcgccgactg cggggtcagg ggcatcaata
     6841 gctcacgcaa gttcgtcaca acacacctcc gagattcctg tcgtcacccg tacctcgcga
     6901 cgctgcgaca ccgttgccct tggggggctg gacgtatcgc gcgagcacat cacgcaccgt
     6961 cggtagcgga gtgccgaggt aaccggactc caggcagtag tccagcacca gtcgtagata
     7021 gtccttgccg gtcgccggac tcgaacgccc gagcagcgca tcagcattcg cggtcgcgaa
     7081 caccgggcgg cgctgaaagt agctggtata cagggcgccg atgcggagga agtactcccg
     7141 ctgcaacgga tccaggtctt cggcggtgaa ggtggattcg gggacgaagg tcgcgacccg
     7201 gaacgacggg tactccgcca gcacgtccga cacctcccgc agcgacaacc cgccacggcc
     7261 gagggcatgg aaggtgcgcc cactcgcggt atcgacgttc agggtggcgg tggtgatgac
     7321 atcggcgaca tgatcgaccg gggccagcgc gatcgtcgcg tcgtagcggc cgggcagcac
     7381 ccgcagcttg ccctccacga tcagtttcac caccgtgtac agattcttga aatcccggat
     7441 ccgcccggtc ccggtcgcac ccgtcacgat cccgggccgc acgatcaccc accgcaaccc
     7501 cgctgcgccc cgcaccaggt gttcggcacg gaacttgctg tcctcatacc ggttaccgaa
     7561 cccggaccca ccctcgagtt ccgcctcctc gatggtgccg ccctgcatgc cgcacacata
     7621 cgcggtactc acatacacca gcggcacacc ccacgcgagc gccaactcca ccgcgtgcgc
     7681 ggtggcttgc acgttgagtt cctgatagtc ggtgtcggtg gcgtcgaagg cggtagtcgc
     7741 ggcactgtgc acgatcaccc ccacccgctg atcatcaacc acacccggct cgttgggggt
     7801 gaggccgagg ccgggacggc ggatgtcacc gggcaccgcg tgcacgccgt cgaggacggt
     7861 gccgtcgttg cggaggatgc gggacgtggt gtgccgcacc gccacaactt cgcgtccagc
     7921 ggccagcagg cgggcggcga cctccgcacc aaccaaaccg gtggcaccgg tcagcaggac
     7981 cgccccggtg ccggtcatgg ctggctccgt tcactcaggc cctgttcgat cagggcgatc
     8041 acgtcggtga cgcggtggac tccggcgagt tggtcggggc ggtagcgggg caggtcgaac
     8101 gcctgctcca acaccagggt gagttccatc agacgcaacg agtcgaagcc caggtcggtc
     8161 acgagttgct gatcgtcggt gaccgccgat cccgggtcgg gtgccatggc agcgatcaac
     8221 tcccggacgc gggcgggggt gctgtggtcg atatcggtca ctgcgagcct ccttggtgtg
     8281 gttggtggcc tccggtggtg agcaggtcgc gggcctcgtc caggggtagg tcggtgtcca
     8341 ggtacccggc gatgcgcagc cagtcgtaac tgatgtcgag ggtgcgccgc cactgctcac
     8401 ccgagtcgaa cgacgccgga aaacccgtgc cacccaccac aacacggata cgggcgataa
     8461 ggtcgtcgag gaccgccatc ttccgtaacg cgtcgtcccc gatctgcggg atcggggaga
     8521 accgcacctg cccggcctgt tccaccaccg cgatcagatc ggggggcgtg gcgggtacca
     8581 ggcgacgcaa agtctgggtg ggggtggtgt cggcgggcag cgggtggatc ccgtacgcgg
     8641 acaacagcat ccggacacac atggcgacca cccggtgcgc ggcgatgtcg gcgaccgcgc
     8701 cctgcccgct cttgacggca ccggccaggt cctcgcgggc gttctccacc accacgttcg
     8761 cccacaccaa ctccgaggca cacgcgatga accgggtcgc gggcaacggc gacaccgcca
     8821 ccccaccatc acgagcagca ccagccaact cgatggcagg ggcgaccggg cacgggacag
     8881 gggtgtaggg gcgcaacggt ttacacatcg ccaacgcggg ggtgatcgcg cccgcgcccc
     8941 gccaggccaa cccgaccagc cccaatcgga tgaattctgc gagttcgggg ccgatggcgg
     9001 cgcgggtgag gatgtcgcgc agggagcggc gtacgacgac cgagcgccag gcgtgtgcgg
     9061 cgcggtcgga cagggcgaac acgtcgcggt cgtcgcgcag aacgtgcaca cggtccccga
     9121 ccgcccacgt cgtcaccttc gccaccctgg tcaacagaat ctggtcggag tcgtcggcga
     9181 cctccacacc gaaccccacc gccagggcga gaatcgttgc cagatcagca gattcatcca
     9241 gcgagcggta gcggccgatc aacgctgctg tgtccggtcg agcggccgcg agtcggttca
     9301 gttggtgggg aagccatttc gtctccaggt aggtttcgcc gtgttcggcc agccatgcct
     9361 tcaccgcttg caacagcccg tcgcgcgccc aagccacggc ctcgtcgtgt tcacccaacg
     9421 cgtgcaaggc gaccgcgtag cgcagggcgt ggcgggcgcg ctcggcccac cagcccgcca
     9481 cgacccccgc gaacacggag cgcggcaaca actcccggag cccgtccagg tccagggttt
     9541 ccgggccgga gcggaggacg gtggcgtgca ggaagcgttg gcagcggttg agcagatcct
     9601 cctccacctc cccgcgggcg acctggtcga gttggtggcg gagttgggtg tgggagcggg
     9661 tgcggatttc gatgcggcgg ccgtcgagga acaacaccgt cggcagcgtc ggcatctgtt
     9721 cgtcgccggg ggccacgatt aggaagtcga tatcggagcc gtcgtttccg aacccctcgg
     9781 ccagcgaccc ctcgaacaac accgccgaat ccggcgggac tgccaccatc gccaatgccc
     9841 gatccagtaa ctcgtgcaag cgatccagag gctcgggccg atcggcgccg gtggcctcga
     9901 tatcgggggt gtcgtggacg tgggcgccgt cgaggctgtt gtcgcgatac agctgccaca
     9961 tgtggcggcg gcgtggtttg ccgctggagg tgcggcggat gaacccccgc ggcccggtca
    10021 cgatggtgac cgcctgcgcc ggacccaact caccccgaat gatgcggcgc gcctcactga
    10081 tccagtcccc cggcgcggtt tcggcgaaca acgccacgcc ctgcgcaccg gcatcggtga
    10141 gggcgacggc ggcgagtttg cctttggtga tgccggtgtc gcgggcgatg cgggattcga
    10201 ggtcttccat gaacaccgac cggccccgca ccttcacact cgtgcccatc cgccccagca
    10261 cgaacacctc accgtggtaa aggaatccgg cgtcaccggt gaacaggtca ccgttctcga
    10321 tgcgggtacc gctaccggtg gtttcctctc cggaataccc gagggcgacc gagtccccgg
    10381 ccaccaccat ctcgcccaac gtcccgtcgg gcaggtcgtg gccgtgctcg tcgacgaccc
    10441 gcacccgcga ctcgggggtg gagtacccca ggccggtgat ccagcccggc ccgtccaccc
    10501 acctcccgtc accgagcgcg gcttcggcgg tgactcggat ggggtacccg aaccgcaggc
    10561 tcccggtgtc caggcgtagc gcggtgaggg gccggtggcg ggtggaggag gtcaccatca
    10621 acgtcgcctc cgccaagccg taggcgaggg tgtaggcgtc ggcggagaag ccctgcgggc
    10681 cggtcaattc cgcgaacgcc cgcaaatccg ccacctccac cggctccgac cccaccgcca
    10741 acgtccgcca ccccgacaga tcgagatcct ggatttgttc gggggtgacg cggtgggcga
    10801 catacccgag cgcgaacgac ggggaggggg cgtggcgggc tatcgtcatc gcccgtaacc
    10861 agcgcaccgg gtcgcgcacg aactgatccg gccgcatcaa atacaggtcg gcttggttgg
    10921 cgacggtggt gaggaatgcg ccgaccaggc ccatgtc
//
