LOCUS       NZ_KV411303            11836 bp    DNA     linear   CON 12-FEB-2024
DEFINITION  Nocardia terpenica strain IFM 0406 scaffold1, whole genome shotgun
            sequence.
ACCESSION   NZ_KV411303
VERSION     NZ_KV411303.1
KEYWORDS    WGS; RefSeq.
SOURCE      Nocardia terpenica
  ORGANISM  Nocardia terpenica
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Nocardiaceae; Nocardia.
REFERENCE   1  (bases 1 to 4641778)
  AUTHORS   Buchmann,A., Eitel,M., Koch,P., Schwarz,P.N., Stegmann,E.,
            Wohlleben,W., Wolanski,M., Krawiec,M., Zakrzewska-Czerwinska,J.,
            Mendez,C., Botas,A., Moris,F., Cortes,J. and Gross,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-APR-2016) Pharmaceutical Biology, University of
            Tuebingen, Auf der Morgenstelle 8, Tuebingen 72076, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 30-JUL-2014
            Assembly Method                   :: CLC NGS Cell v. 7.0.4
            Genome Coverage                   :: 118.0x
            Sequencing Technology             :: Illumina HiSeq; PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/12/2024 01:56:15
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,832
            CDSs (total)                      :: 8,760
            Genes (coding)                    :: 8,562
            CDSs (with protein)               :: 8,562
            Genes (RNA)                       :: 72
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 198
            CDSs (without protein)            :: 198
            Pseudo Genes (ambiguous residues) :: 0 of 198
            Pseudo Genes (frameshifted)       :: 40 of 198
            Pseudo Genes (incomplete)         :: 174 of 198
            Pseudo Genes (internal stop)      :: 9 of 198
            Pseudo Genes (multiple problems)  :: 23 of 198
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 16:51:21
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 1681210
            Orig. end                         :: 1693046
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            KV411303.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..11836
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="8"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..11836
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..11836
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:6836](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..6836
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     misc_feature    469..482
                     /note="TFBS match to ANR, Anaerobic transcriptional
                     regulator, confidence: weak, score: 19.57"
                     /tool="antismash"
     gene            complement(1048..1986)
                     /gene="cysD"
                     /locus_tag="AWN90_RS07555"
                     /old_locus_tag="AWN90_07595"
     CDS             complement(1048..1986)
                     /EC_number="2.7.7.4"
                     /GO_function="GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA]"
                     /GO_process="GO:0019419 - sulfate reduction [Evidence IEA]"
                     /codon_start=1
                     /gene="cysD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006369832.1"
                     /locus_tag="AWN90_RS07555"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AWN90_07595"
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="WP_067578906.1"
                     /transl_table=11
                     /translation="MIRGYVALRDLTKYEISHLRVLESESVHVIREAVATLHHPVMLFS
                     GGKDSTVMLRLAEKAFWPAPVPFPAMHIDTGHNFDEVIEFRNRVIANSSMRLIVAKVQD
                     DIDAGRVVDEAGVGASRNRLQSATLLRSIRAGGFDAVFGGARRDEEKSRAKERIFSLRN
                     KFGAWNPHDQRPELWSLYNGRHAEGEHVRVFPLSNWTELDIWQYIESESIPLPSIYFAH
                     CREVVPRNGVLLAKTRFLAMAPGEISYRDSVRFRTVGDATCTGCIPSRADTVKKVIAEL
                     AVTRSTERGATRADDRISETGMEDRKREGYF"
     misc_feature    complement(1342..1344)
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     misc_feature    complement(2097..2117)
                     /note="TFBS match to MntR, Manganese transport regulator,
                     confidence: weak, score: 21.27"
                     /tool="antismash"
     gene            2334..3632
                     /locus_tag="AWN90_RS07560"
                     /old_locus_tag="AWN90_07600"
     CDS             2334..3632
                     /GO_function="GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /GO_function="GO:0005506 - iron ion binding [Evidence IEA]"
                     /GO_function="GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /GO_function="GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) p450"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1007: cytochrome P450"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /locus_tag="AWN90_RS07560"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AWN90_07600"
                     /product="cytochrome P450"
                     /protein_id="WP_156673926.1"
                     /sec_met_domain="p450 (E-value: 2.5e-13, bitscore: 47.5,
                     seeds: 50, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MCAEVGSRKGGEQKWSTVTELEYNWCRPAASGGTSRAKVRRTGKS
                     LKFDPLSPEWIDDPYVFYSRLRHNEPVLRDDNLGAYLLFSYADCAAVLRTTDDFATDPA
                     RLGRDFPAIRKSIQLIDPPENTALRKATAEAIHAGFSALSATTGGLTEVVAKALFFARH
                     ADRPVDLITEFSKPLCLWVMCGVLGARQPDPDWFAEVSDRMVFGMDYGFRPDALEPSLS
                     AKRELSELTDSWLDEHRDSVFFGTLLGVATTYTRDVVANSVRVVLHAGYSSVSGLIENL
                     LLHLLVEREMFEEHRERLLESKAARITAIEEMIRYDSPVQVSKRLTVEDLELSGVDVPR
                     GADVIALIGAANRDPTVFAEPDQMNLLRTPNPHLGFGRGPHACLGANLSIELGSAVLKA
                     FLVEQPNMRLAAHPVRRKTASIRGLASLPVWLE"
     gene            3637..4980
                     /locus_tag="AWN90_RS07565"
                     /old_locus_tag="AWN90_07605"
     CDS             3637..4980
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="AWN90_RS07565"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="AWN90_07605"
                     /product="hypothetical protein"
                     /protein_id="WP_156673927.1"
                     /transl_table=11
                     /translation="MSLSIATAHTALTNDHTAFCRGVRARFPLGREGRHGLPAFRRVVV
                     IAASSRGGSSLLYELLRSTGAFLSLGGEHSVLYKLHGLGLPQGQDAHDGCIADDADLTG
                     FHADLAADVTTGTEPDLIDPRDWDSATLTDRFVRMFAQQWPQCPASAEDIWKIVHEVIA
                     GRLRHGLANRSALLMVCVRALRTAGWQIDPWYFDVDPAIIRAAFPSVPPPTGPPPGLAR
                     TVEAPPFLVPSLAPLPDTNDLQRPLLLKASMDAYRLAALPRLFPDAELTVIHLVRNPAA
                     SVNGLIDGWLDRGFFSHNLNGRADLRIPGYSGPADWSMQWWNFDLPPGWRNLVDRPLPW
                     VCAAQWCAAHSHILDALEASALPALRVQAEDIMDGATRRATIDTILQHCRLRARRPARS
                     RVVMASRIPEPGRWRRRRAILEPMISSGEIRSCAMRLGYDSTAGDRWK"
     misc_feature    4417..4419
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            5001..6836
                     /locus_tag="AWN90_RS07570"
                     /old_locus_tag="AWN90_07610"
     CDS             5001..6836
                     /GO_function="GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: TIGR03975"
                     /gene_functions="other (smcogs) SMCOG1277: Radical SAM
                     domain protein"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:TIGR03975.1"
                     /locus_tag="AWN90_RS07570"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AWN90_07610"
                     /product="RiPP maturation radical SAM C-methyltransferase"
                     /protein_id="WP_067578912.1"
                     /sec_met_domain="TIGR03975 (E-value: 5.6e-175, bitscore:
                     581.1, seeds: 14, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKILLVSMPWNLLEMPSLPVGTLRVVTQRCRDRHEVADAYLNLAW
                     AEHLHDATDGHIGVPEYDYVANVGVWHGMGDWIFTPALYGAPEWRRDRYEAYLQQRGVR
                     PGASVEMAQHSGAFIESHAQRIADQGYDLVGFSSTFQQNVPSLALAKQLKRLRPETTII
                     MGGGNCDIPMGPAIHRNFPFIDYVLSGEAEQSFVDFLDHLSGWQSTSAVRGLSWRAADG
                     VTVTNPPAPSPAMHTVPAPDYSEWFARIGASAVRDSVRPKLVYEAARGCWWGEKHTCTF
                     CGLNGLAMKFRSRPADQVMTHLHDLIALHRILDVVAVDNILDMEYLRTLLPRLAEQDWD
                     LHFYYEVKANLREEDIGLLRRAGLVQIQPGIENLSSRVLSIMDKGVHATQNVKLLRDCE
                     EHDLTVDWNYLYGFPGETEADYAIVLRQLPALAHLQPPGGSVRILLERYSPYFQRPELG
                     FEHRRPAALYNHVYDLDQEQMSDVAYQFDTERCGIGIDYEHAVRAAIAAWQQAYPSSSL
                     VARELPDRTLIVEDRRVGWPVRDIKLEPGPEAELFRYLRTPHTVASAARATGLAPRVVD
                     AVVDEWERQGLVFHEGGRVVSLPTGRASIKSAIDR"
     gene            6873..7325
                     /locus_tag="AWN90_RS07575"
                     /old_locus_tag="AWN90_07615"
     CDS             6873..7325
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF039872.3"
                     /locus_tag="AWN90_RS07575"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AWN90_07615"
                     /product="DUF5825 family protein"
                     /protein_id="WP_156673928.1"
                     /transl_table=11
                     /translation="MHANMHHPDEFLAFVADLRDMTARGESVRWTCAADADTVAPLQHL
                     APPLWLKPGVEPTVWRARHRPCQFYFRRGPGFVIIHDERSGSAVETLLDDPEHLVLFER
                     LHHPGALRPGSATSALRAAGLLFELGDKGVVLPYRLSRLALPTKLL"
     misc_feature    7146..7148
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            7362..8144
                     /locus_tag="AWN90_RS07580"
                     /old_locus_tag="AWN90_07620"
     CDS             7362..8144
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /NRPS_PKS="Domain: TauD (25-246). E-value: 4.4e-21. Score:
                     67.8. Matches aSDomain:
                     nrpspksdomains_AWN90_RS07580_TauD.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) TauD"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF014698.4"
                     /locus_tag="AWN90_RS07580"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AWN90_07620"
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="WP_082870649.1"
                     /sec_met_domain="TauD (E-value: 3.2e-19, bitscore: 67.8,
                     seeds: 119, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNDPAASAVLAFTAGPTALADKMSKALTAFGYVHVAGVPADFDRL
                     SVLSLLGAPVRQYQGELIRDIKPDPAMQAFEVSALNMKPLVPHTEHFEFAGLPPRYIAL
                     WAVTPAQGPGGETTLADGYELLGTFSPEQRAAMSVREFEWRSPMSLTAEGVHEAARHPI
                     AEHTPWGIVLRFSGREMRYRTQDGEYADDSLLTEYRDRGLRHFERTCRSVKIAQNALLV
                     WDNWRMYHSRNGFSDPRRHLRRVMLDHCPTARFPLPAN"
     aSDomain        7437..8099
                     /aSDomain="TauD"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_AWN90_RS07580_TauD.1"
                     /evalue="4.40E-21"
                     /label="AWN90_RS07580_TauD.1"
                     /locus_tag="AWN90_RS07580"
                     /protein_end="246"
                     /protein_start="25"
                     /score="67.8"
                     /tool="antismash"
                     /translation="ALTAFGYVHVAGVPADFDRLSVLSLLGAPVRQYQGELIRDIKPDP
                     AMQAFEVSALNMKPLVPHTEHFEFAGLPPRYIALWAVTPAQGPGGETTLADGYELLGTF
                     SPEQRAAMSVREFEWRSPMSLTAEGVHEAARHPIAEHTPWGIVLRFSGREMRYRTQDGE
                     YADDSLLTEYRDRGLRHFERTCRSVKIAQNALLVWDNWRMYHSRNGFSDPRRHLRRVM"
     gene            8290..9519
                     /locus_tag="AWN90_RS07585"
                     /old_locus_tag="AWN90_07625"
     CDS             8290..9519
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="AWN90_RS07585"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="AWN90_07625"
                     /product="circularly permuted type 2 ATP-grasp protein"
                     /protein_id="WP_171982950.1"
                     /transl_table=11
                     /translation="MAELSVPLDAWPFVLTPQFLSRGALAALRTGLTAILDGIDIVLRE
                     KFQHDPGRLAAALRLPPRQRDVYRIGAAQDWARIARPDVVFDSSGAPWFVELNAGTPLG
                     GIAMSAVLARMYDAWPESAEYLRGVGATYVDTVRALAEHLAEVDRLDRSRLMVVAYWGH
                     EDDNMPSHSYLGLVRALGRYGITAVAAAVEDLDLDGEYIRYDGRRVDALYRFFDESDGT
                     PGLKDDRWRHLVEHVDRGSVSLVGNLVGNVFVNKGFLAILSEAAASGSLPSSLAERINA
                     ALPWTRMIDDVAETVAQQRSAYVLKPADGCCGEGLVFGPATEQSAWEQAIDDAVTGEEL
                     WVVQRVVRPPVLRLASLGTGGMTFSEFSTSTGVFAVGRRFAGAIRRCDPTLGLNVTPSL
                     GAAQGSVHVL"
     misc_feature    8665..8667
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            9509..10900
                     /locus_tag="AWN90_RS07590"
                     /old_locus_tag="AWN90_07630"
     CDS             9509..10900
                     /GO_function="GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /GO_process="GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /NRPS_PKS="Domain: Aminotran_1_2 (122-272). E-value:
                     2.1e-08. Score: 25.6. Matches aSDomain:
                     nrpspksdomains_AWN90_RS07590_Aminotran_1_2.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_1_2"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1180: Decarboxylase, pyridoxal-dependent"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF012503.4"
                     /locus_tag="AWN90_RS07590"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AWN90_07630"
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="WP_067578919.1"
                     /sec_met_domain="Aminotran_1_2 (E-value: 1.5e-06, bitscore:
                     25.6, seeds: 48, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSSEETGDRPGALPGLHTYAEDFVQLMLSTVRRRMANGPLPLGSP
                     GGQAELKKALDGVIGPVGRDPRDVLRIYEEVLEPTFVAMDNPTFLAFFQGAPSQAATLF
                     DMLVSSVALRGVTWLGASGAVAAENQVLDLLAARAGLPDTAGGCFLSGGSAANLSALVV
                     ARDTARRRLGLDDSRQVDIIVSDQAHPSVLHAIRIIGARPIVVTTPSCRLTAAGLETAL
                     RERVVAPAAVIATAGTPNAGLVDDLAAIADVASRSGIWFHVDAAYGGGAALFDPDSRAV
                     FAGIERADSLVVDPHKWLFSPYDCSALIYRRPQSARAVHTQRAAYFDVIHDAEHLEWNP
                     SDFAYHSTRRPRGLPLWFALAVYGTAAFEDAVRTSLRTARELAALVESTPYLESVLPPE
                     LSVVLVRRKGWSPRDYQLWSAGLLERQEGFVAPTIWAGETVARFAILNPRTSVDMLEKI
                     VASMA"
     aSDomain        9875..10324
                     /aSDomain="Aminotran_1_2"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_AWN90_RS07590_Aminotran_1_2.1"
                     /evalue="2.10E-08"
                     /label="AWN90_RS07590_Aminotran_1_2.1"
                     /locus_tag="AWN90_RS07590"
                     /protein_end="272"
                     /protein_start="122"
                     /score="25.6"
                     /tool="antismash"
                     /translation="GAVAAENQVLDLLAARAGLPDTAGGCFLSGGSAANLSALVVARDT
                     ARRRLGLDDSRQVDIIVSDQAHPSVLHAIRIIGARPIVVTTPSCRLTAAGLETALRERV
                     VAPAAVIATAGTPNAGLVDDLAAIADVASRSGIWFHVDAAYGGGAA"
     misc_feature    10757..10759
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     misc_feature    11280..11293
                     /note="TFBS match to ANR, Anaerobic transcriptional
                     regulator, confidence: weak, score: 19.57"
                     /tool="antismash"
ORIGIN
        1 ccggtactcg tggcaatgag gtatcggctc gacgagcgta attcggagtt gtcggcgagc
       61 caacacacgt tggcatcgat ctcgcaaccg acgaaggggc ggtttctcag ccgtgccagc
      121 atgtcgccac gtgccaggtc gatgtcgtcg gtcaacgata ccgataccgc catcggggga
      181 aaggcctctg tcatcttttc gccgcccgga ccccagatct gttcaatcct cgagatcacc
      241 ccaccgggta atgcgacgat ctcgtcaccg gggcggaacg tgcccccggc gacggtgccg
      301 gcatagctgc gatgaccgac gcggtcaccg gactgcgggt cgaggacata ctgcaccggc
      361 agtctagcgt ctatcaggtt gcgattcgag gcgatatgca cgtcctcaag atggcgcagc
      421 agcgaaacgc cctgatacca ccgcatatgc ttcgaacgat cgacgacgtt gtcgccgaac
      481 aaggcagaca ccgggacgaa ggtcagatcg tggacgtcaa gtttggatgc gaaggagccg
      541 aattcggcac ggatctcctc aaatcgttcc tgcgaccaat ccacaagatc catcttgttg
      601 acacacaaca ccaggtgcgg aatttctagg agggcggcca agaaggcatg ctgtctggtc
      661 tgatccagta cgcctttgcg agcatccaac aggatcaaca ccagatccgc agtggacgcg
      721 ccggtcacca ggttgcgggt gtactgccta tgtcctgggg tgtcggcgat aatgaacttg
      781 cgctgggccg tggcaaagaa ccggaatgcg acatcgatgg tgatgccctg ttcgcgttcg
      841 gcccgtaatc catcggtcaa cagcgccagg ttcgtgtatc tgtcaccccg ctggttgctg
      901 gcacgctcca ccgcgtcgag ttggtcctcg gggatcgact cggagtcgta caaaaggcgg
      961 ccgatgagag tcgattttcc gtcgtcaaca ctaccggcgg tcgcgagtcg cagcagctcc
     1021 cggggccggg tcattggatc tcccccatca gaagtagccc tctcgcttgc ggtcttccat
     1081 tcccgtctct gagatccgat cgtcggctcg cgtcgctccg cgctcggtcg agcgggtcac
     1141 cgcaagctcc gctatcactt ttttcacggt gtcggctcgg ctcggaatgc atcctgtgca
     1201 ggtggcatcc ccgaccgtcc ggaaacgtac cgaatcccgg tacgagatct cgcccggtgc
     1261 catcgccaga aaacgtgtct tcgccagcag cactccattt ctgggcacta cctcccgaca
     1321 atgagcgaag tagatgctgg gtaatgggat ggattccgac tcgatgtatt gccaaatgtc
     1381 gagttcggtc cagttgctca acgggaatac ccggacgtgc tcgccctcgg catgcctacc
     1441 gttgtacagg ctccacagtt ccgggcgttg atcgtgtggg ttccacgcgc cgaacttgtt
     1501 tcggagcgaa aagatgcgtt ctttggctcg cgacttctcc tcgtcccgcc gcgcaccgcc
     1561 gaatacggca tcaaaaccac ccgcccggat ggaacgcagc agagttgcgc tctgcagccg
     1621 gttgcgagag gcgccaacac cggcctcatc gactacccgg ccagcatcga tatcgtcctg
     1681 caccttcgca actatcaatc gcatactcga attagcaatc actctgttgc ggaattctat
     1741 gacttcgtcg aagttgtgac cggtatcgat gtgcattgcg ggaaatggta caggtgccgg
     1801 ccagaatgct ttctcagcga ggcgcaacat tactgtggag tctttgcctc cggagaacag
     1861 catcacagga tgatgcagcg tggcgacagc ttcgcgaatc acgtgtaccg attccgactc
     1921 caggaccctc agatgtgata tttcgtattt cgtgagatca cgcaatgcaa cgtacccccg
     1981 gatcaagcgg acgtatgtcc gtctccacta tgtggtgcca taacggcgct tgtcaagcag
     2041 atcgaagcac cactcagctt gtggcctcgt gacacgttct aagatcattt cacccccgca
     2101 aagccatagc taagttcgga cattctcgaa ggacttagga gatggagttc ttccacttga
     2161 caagaactat cggacgccgc atagtggagc cccatacatc gaactaccgg gtcgcaagct
     2221 cagcttcagt tcgataatgt ctttggggcc agatctgcaa gggggatgca ttgcatagcg
     2281 tggcgacctt cgccttgcaa cttcttactg acacctgact gcacacgctg catttgtgtg
     2341 cggaagttgg atctcgaaaa ggaggtgagc agaagtggag tacggtcact gagcttgagt
     2401 acaactggtg ccgcccggcg gcgtcgggcg gcaccagccg cgctaaagtc cgacgaacgg
     2461 ggaaatcctt gaaattcgat ccgttgtcgc cggaatggat tgacgacccc tacgtcttct
     2521 attctcggtt gcgtcacaac gagcctgtac ttcgagacga caatctcggc gcgtacctgt
     2581 tgttcagcta tgccgactgt gccgcggtac ttcgaaccac ggacgatttc gccacggatc
     2641 cggcgcggct gggccgagat ttcccggcca ttcgcaagag cattcagctc atcgatccgc
     2701 cggagaacac cgcgctgcgg aaggcgactg cggaagcgat acatgccggg ttctccgcct
     2761 tgtccgcaac taccggcgga ctcaccgaag tggtcgccaa agccctcttc ttcgcgcggc
     2821 acgccgatcg accggtcgat ctgatcacgg agttctccaa gccgttgtgc ctgtgggtga
     2881 tgtgcggtgt gctcggtgcc cggcagccgg atccggattg gttcgccgag gtgagcgatc
     2941 ggatggtctt cggaatggac tacgggttcc gccccgatgc gctggagcct agtctctccg
     3001 caaagcgcga actatccgaa ctcaccgact cctggctgga cgagcaccgg gactccgtct
     3061 tcttcggcac tctgctcgga gtcgccacca cctacacgcg cgatgtcgtc gccaattcgg
     3121 tgcgggtggt tctgcacgcg ggttactcct cggtcagcgg actcatcgag aatctcctcc
     3181 tgcacctgct ggtcgaacgc gaaatgttcg aagagcatcg ggaacggctg ctggagtcca
     3241 aggcagcccg tattactgcg atcgaggaga tgattcggta cgacagtccg gtacaggtat
     3301 cgaaacggct cacggtcgaa gacctggagc tgtccggcgt cgacgtgccc cgcggtgcgg
     3361 acgtgatcgc gctgatcggg gcggccaacc gcgacccgac cgtattcgcc gagccggatc
     3421 agatgaatct gttgcgaaca ccgaacccac acctcggttt cggacgcggc ccccatgcgt
     3481 gcctgggggc caatctctca atcgaattgg gttccgccgt gctcaaggcg ttcttggtgg
     3541 agcaaccaaa tatgaggttg gctgcacatc cggtacgccg gaagaccgcg agtattcgag
     3601 ggctcgcgag cctgcccgtg tggttggagt gaaagcgtgt ccctctccat cgctacagcc
     3661 cacaccgccc tgaccaacga ccacaccgcc ttctgccgcg gagtccgggc ccgcttcccg
     3721 ctcggccgag aggggcgcca cggcctgcct gccttccgtc gtgtcgtcgt cattgcggcc
     3781 tcgtcacgag gaggtagcag cctgctctac gagctgcttc gctccaccgg cgccttcctc
     3841 agcctcggcg gcgagcatag cgtgctgtac aagctgcacg gactcggact accacaaggg
     3901 caagatgcgc acgacggttg catcgccgac gatgccgacc tcaccggctt ccacgcagac
     3961 ctcgcggccg atgtaacgac gggaaccgag ccggacctga tcgacccccg agactgggac
     4021 tccgcgacgc tcacggaccg cttcgtgcgg atgttcgccc agcaatggcc ccaatgcccg
     4081 gcctccgccg aggatatctg gaagatcgtg catgaggtga tcgcgggacg cttgcggcac
     4141 ggcctggcga acaggtccgc gctgttgatg gtatgcgttc gggcactgcg gacggccggt
     4201 tggcagatcg acccctggta tttcgacgtc gatccggcaa tcatcagggc ggcgtttccg
     4261 agtgtgccac cgcccaccgg cccgccgccg gggctggccc ggacggtgga ggcaccgccc
     4321 ttcctggtac ccagcttggc gccgctgccc gatacgaacg acctgcaacg cccactgctg
     4381 ctcaaggctt cgatggatgc ctaccgcctc gccgcgttac cgcgcctgtt ccccgatgcc
     4441 gaactgactg tcattcactt ggtccgcaac cctgcggcct cggtgaacgg cctcatcgat
     4501 ggctggctcg accgggggtt cttctcgcac aacctaaacg gacgtgccga cttgcgaatc
     4561 cccggctact cgggacccgc tgactggtcc atgcagtggt ggaacttcga tctgccgccc
     4621 ggctggcgga atctggtcga ccggcccctg ccatgggtat gcgccgcgca atggtgcgcc
     4681 gcacattcac acatcctcga cgcgctggaa gccagcgcgc tacccgcact gcgggtacag
     4741 gccgaggaca tcatggacgg ggccactagg cgggcgacga tcgacacaat tctgcagcat
     4801 tgccgcctgc gagcccggcg accggcacgg tcacgggtgg tcatggccag ccggatacca
     4861 gagccgggac ggtggcgtcg gcgtcgcgcg atattggagc cgatgatcag cagcggcgaa
     4921 atccggtcct gcgcaatgcg tctggggtac gactcgaccg ccggtgaccg gtggaagtaa
     4981 cgatcacgag aggaggggtt gtgaaaatac ttctggtctc gatgccctgg aatctgctgg
     5041 agatgccttc gttgccggtg ggcacactgc gggtcgtcac ccaacggtgc cgcgatcgtc
     5101 atgaggtcgc cgacgcctat ctcaacctgg catgggccga gcacctgcat gatgccacgg
     5161 acggacacat cggggtgccg gagtacgact acgtggccaa tgtcggggtc tggcacggca
     5221 tgggcgactg gatcttcact ccggcgctgt acggtgcgcc ggagtggcgc cgtgaccgat
     5281 acgaggccta tctgcagcag cgcggagttc ggccgggcgc gtccgtggaa atggcccagc
     5341 actcaggtgc attcatcgaa tcgcacgccc agcggatcgc cgatcaggga tacgacctgg
     5401 tcggcttcag cagcaccttt cagcagaacg tgccctcgct ggccctggca aagcagctga
     5461 agcggctgcg gccggagacg accatcatca tgggcggcgg caactgcgac attccgatgg
     5521 gcccagccat ccaccgcaac tttccgttca tcgactacgt cctgagcggt gaggcggagc
     5581 agtccttcgt ggacttcctg gaccacttga gtggttggca atcgacgagt gccgtccgcg
     5641 gcttgtcctg gcgcgccgcc gacggtgtca ccgtcaccaa cccaccggct ccgtcgccag
     5701 ccatgcatac ggtcccggcg cccgactact ccgaatggtt tgccaggatc ggtgccagtg
     5761 ccgtgcgcga ctccgttcgg cccaagctcg tgtacgaggc tgcccgcggc tgctggtggg
     5821 gagagaagca cacctgcacg ttctgtggac tgaacgggct cgccatgaag ttccgctcca
     5881 gaccagccga ccaggtcatg acccacctgc acgacctgat cgccctgcac agaattctcg
     5941 atgtcgtggc tgtggacaac atcctcgaca tggagtattt gcgcacgctg ctgccgcggc
     6001 tcgcagagca ggactgggac ctgcacttct actacgaggt gaaagcgaac ctacgggagg
     6061 aggacatcgg gctgctgcgt cgtgccggac tcgtccagat ccagcccggc atcgagaacc
     6121 tcagctcccg cgtgctgtcc atcatggaca agggcgtaca cgcgacacag aacgtcaagc
     6181 tgttgcgcga ctgcgaggag cacgacctga cggtcgactg gaactatctg tatggcttcc
     6241 caggggaaac cgaggccgat tacgccatag tcctgcgcca gcttccggcg ctcgcacacc
     6301 ttcagccgcc gggcggcagc gtgcgaattc tactcgagcg atacagcccg tacttccagc
     6361 gaccagagct cggcttcgaa caccgtcgac cggccgctct ctacaaccac gtttacgacc
     6421 tggaccagga acagatgagc gacgtcgcct accagttcga cactgagcgg tgcggtatcg
     6481 gtatcgacta cgaacacgcc gtgcgggccg ctatcgccgc atggcagcag gcttacccgt
     6541 cgagcagctt ggtggcacga gagcttccgg accgcacatt gatcgtcgag gaccgccggg
     6601 tcggttggcc ggtgcgcgat atcaagctcg aacccgggcc cgaggccgag ctgttccgct
     6661 atctgcgcac gcctcacacc gtcgccagtg ctgctcgcgc gaccggcctc gctccgcggg
     6721 tggtcgatgc tgtggtcgac gaatgggagc gccaaggtct ggtgttccac gagggcggca
     6781 gggtcgtatc gctgcccacc ggccgcgcca gcatcaagtc cgcgatcgat cggtaggcca
     6841 tgctgccagg acagtccacc cccgacatcc cggtgcacgc caacatgcac cacccggacg
     6901 aattcctcgc cttcgtggcc gatttgcgcg acatgactgc gcggggcgag tccgtgcggt
     6961 ggacgtgtgc cgccgacgcg gacaccgtgg ccccgctgca gcacctggcg ccgcctctgt
     7021 ggctcaagcc cggtgtcgag ccgaccgtgt ggcgcgcacg gcaccgcccc tgccagttct
     7081 acttccgccg cggtcccgga tttgtgatta tccatgacga gcgttcgggc agcgccgtcg
     7141 aaaccttact ggacgatccg gaacacctcg tactcttcga acgactgcac catcccggcg
     7201 cccttcggcc cgggtcggcc acatcggcgc tgcgcgccgc ggggctgctg ttcgaactcg
     7261 gcgacaaggg cgtcgtcctg ccgtatcggc tgagccgcct tgcactgccg accaagttgc
     7321 tgtgatgcga aacgacatgt cgagaccgag gtgaccagtc gatgaatgat cccgccgcat
     7381 cggcagttct cgcgtttacg gccggaccga cggccttggc ggacaaaatg tccaaggccc
     7441 tcaccgcatt cggatacgtc cacgtcgccg gtgttccggc cgacttcgat cgtctgtccg
     7501 tgctctcact tctgggggct ccggtccggc agtaccaggg cgagctgatc cgcgacatca
     7561 agccggatcc ggcgatgcag gcgttcgagg tctccgccct gaatatgaag ccgctggttc
     7621 cgcacaccga acacttcgag ttcgccggtc tgccgccgcg gtacattgcg ctctgggcgg
     7681 tgacacctgc acaggggccg ggcggggaga caacgcttgc cgacggatac gagttgctgg
     7741 gcaccttctc cccggagcag cgagcggcga tgtcagttcg cgagttcgaa tggcggtcgc
     7801 ctatgagcct gactgccgag ggagtacacg aggccgcgcg ccacccgatc gccgaacata
     7861 cgccgtgggg catcgtgttg cgcttctccg gcagggagat gcgttaccga acacaggacg
     7921 gtgaatacgc cgacgactct ctcttgaccg agtaccgcga tcgcgggctc cgccacttcg
     7981 aacgaacctg ccgctctgtc aagatcgccc aaaatgcgtt gctcgtgtgg gacaactggc
     8041 gaatgtatca ctcccgcaac ggcttttctg atccacgtcg gcacctgcgc agggtcatgc
     8101 tcgaccactg ccctaccgcc cgtttccccc ttcccgccaa ctgaggtaag cgtggactcc
     8161 aatgacctat gccttccggc gatcaccgac caggaggccg acttcaacca ctggctgcag
     8221 acccgccgga ccgatacgca gcgctacttc gatcccgatc cggcccggcc gggaaccgcg
     8281 ctgtcggaga tggcggagct gagcgtgccg ctcgacgctt ggcccttcgt gctaactccg
     8341 cagttccttt cccgcggcgc cctcgccgcc ctgcgaacgg gactgaccgc gatcctcgac
     8401 gggatcgata tcgtgcttcg ggagaagttc cagcacgatc cgggcaggct cgcggcagcg
     8461 ctgcggttgc caccgcggca gcgcgacgtg tatcggatcg gcgccgctca ggattgggca
     8521 cgcatcgcgc ggcccgacgt cgtcttcgac agctcgggtg ctccgtggtt cgtggagctc
     8581 aatgccggaa caccgctggg cggtatcgcg atgagcgcgg tactggcgcg aatgtacgac
     8641 gcatggcccg aatcggccga gtacttacgc ggcgtcggcg caacgtatgt cgatacggtg
     8701 cgggcgctcg ctgagcatct ggccgaggtc gaccgattgg accggtcgcg actgatggtc
     8761 gtcgcctatt gggggcacga ggacgacaat atgccgtcgc actcctacct cggactggtc
     8821 agggcactgg gccggtacgg catcacggcc gtagccgccg cggtcgagga cctcgacctc
     8881 gacggcgaat acatcagata tgacgggagg cgggtcgatg ccctctaccg cttcttcgac
     8941 gaaagcgacg gcacccccgg actcaaggac gaccgttggc gacacctggt ggagcatgtg
     9001 gaccgtggct cggtctcgtt ggtgggcaat ctggtgggaa atgtcttcgt caacaaaggc
     9061 tttctggcca tcctgtccga ggcggcggcg tccgggagcc ttccgtcctc cctcgccgag
     9121 cggatcaatg ccgcgctgcc gtggaccagg atgatcgacg acgtcgcgga gacggtcgcc
     9181 cagcagcgat cggcatacgt gttgaagccc gctgacggct gctgcggaga agggctggtc
     9241 ttcggccctg ccacagagca gtcggcctgg gagcaggcca tcgacgatgc cgttaccgga
     9301 gaggagcttt gggtcgtaca acgggttgtc cggcctccgg tgcttcgact cgcctccctt
     9361 ggcacgggcg gtatgacgtt ttccgaattc tcgacctcga ccggtgtttt tgccgttggc
     9421 cgacggtttg caggggcgat ccgacggtgt gatccaacct tgggcttgaa tgtgacaccg
     9481 tccctcggcg cggcccaggg gagcgtgcat gtcctctgag gagacgggcg atcggccggg
     9541 cgcacttccc ggattgcaca cctacgcaga ggatttcgtc cagttgatgc tctcgacggt
     9601 gcgacggcgc atggcgaacg gaccgcttcc attggggagt ccgggtgggc aggcggaact
     9661 gaagaaggca ttggacggcg tgatcggccc cgtcgggcgc gaccccaggg atgtactccg
     9721 gatctacgaa gaagtgttgg agccaacatt cgtggccatg gataatccta cgtttctcgc
     9781 attcttccag ggagcgccca gtcaggccgc gacactgttc gacatgctgg tctccagcgt
     9841 cgccctgcga ggtgtgacgt ggctgggtgc gtcgggtgcg gtggccgccg aaaaccaggt
     9901 actggatctg ttggccgctc gcgcaggact gccggacacc gctggaggat gttttctcag
     9961 cggtggttcg gccgccaatc tgtctgcact cgtggtagct cgggacaccg cgcgccgtcg
    10021 actcggcctc gacgattctc gtcaggtgga catcattgtc agcgaccagg cccatccgtc
    10081 cgtgctgcat gccattcgga tcatcggcgc ccggccgatc gtggtcacca cgccgtcttg
    10141 tcggctcacc gcggcggggc tggagaccgc cctgcgggag cgcgtcgttg caccggcggc
    10201 tgtaatcgcg acggccggta cccccaacgc cggactcgtg gacgatttgg ccgcgatcgc
    10261 ggatgtcgcc tctcgatcgg gcatctggtt ccacgtcgat gccgcatacg gcggtggtgc
    10321 agcgcttttc gatccggatt cccgggctgt gttcgccggg atcgaacgtg cggactcact
    10381 tgtcgtcgac ccacacaaat ggttgttcag cccctatgac tgcagtgcgc tgatataccg
    10441 ccgaccgcag tcggcccggg ccgtgcacac tcagcgggcc gcctacttcg atgtcatcca
    10501 cgacgccgag catctggagt ggaacccgag cgacttcgcc taccacagca cgcgtcggcc
    10561 gcgcggactt ccgctgtggt tcgcattggc ggtgtacgga accgctgctt tcgaggacgc
    10621 ggtacggacc agtctcagga ccgccagaga acttgccgca ctcgtggaat cgacgccgta
    10681 cctcgagtcg gtgctcccgc ccgagctgtc cgttgtgctg gtccgtcgca aaggatggag
    10741 cccccgagac taccagttat ggtcggcagg cctgctggag cgacaggaag gctttgtggc
    10801 accgacgatc tgggccggag agacggtcgc gcggttcgcc atcctgaatc cgcggacaag
    10861 cgtagacatg ctggagaaga tcgttgcgag tatggcctga aggagatttc ggatgagctt
    10921 gttgccggtc ggtcaggatt tccgaaggtt ctggctcgct cgagtcacgt ccttcgcagg
    10981 cgaccagatc gcacaggtgg ctctgatcgt tatggccgcg gccgagggac cactggccgc
    11041 cagcatggtc ttgttggcgt tgtcgttgcc gcgcctggtc ggtccgttcg gcggggcact
    11101 tgccgacatc tactcggcga agaagatcat ggtgttgacc gacctcggtc agatggtgct
    11161 attcggcgtg gtcgcggtga ttccgtttca tctcgcggtg agtattccga tcatcgcggt
    11221 ggccaccctg ctcaacacgt tgttcctacc ggccgggcgg cggtcgattc ccgccatggt
    11281 cgatgccgag caactgccga acgcgttcgc ggctatggcg atctgtttca atgccggttt
    11341 cgcggcgggt ccgctcctgg gcggcgcgct gctcaccgtg gtggacgccc gtgtggtcgt
    11401 gctgatcgac gcgctgacgt ttctcgcttc catcgtcctg atcgtccgcc tcgatcttcc
    11461 gaaaacgcca cgtgcggagg agcatcgggt cggttatggg cgcaccctgg cactcggctt
    11521 ccgggaactg gtcggcaaca ggctgctggg ctcggtggca ctcggccttt tcgcggtagt
    11581 ggcattcgcc gcgttgacca cagcatcgtt ggtcttcctg gccagggatg ccttgaacgc
    11641 cccggcgtgg gcctacggtg cactggtagg ggttcacggc atcgggatgg tggtgggccc
    11701 caccgtgttc ctcggtcaag tccgacggag ccgaaatccg ttgcgcacat ggggcttcgg
    11761 gcagggcctg ttcggggtgg gcgcattgac actcgccgtg accccgtggt tggggaccgc
    11821 tttcgtgtcg cagcta
//
