LOCUS       NZ_KV411303            10393 bp    DNA     linear   CON 12-FEB-2024
DEFINITION  Nocardia terpenica strain IFM 0406 scaffold1, whole genome shotgun
            sequence.
ACCESSION   NZ_KV411303
VERSION     NZ_KV411303.1
KEYWORDS    WGS; RefSeq.
SOURCE      Nocardia terpenica
  ORGANISM  Nocardia terpenica
            Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales;
            Nocardiaceae; Nocardia.
REFERENCE   1  (bases 1 to 4641778)
  AUTHORS   Buchmann,A., Eitel,M., Koch,P., Schwarz,P.N., Stegmann,E.,
            Wohlleben,W., Wolanski,M., Krawiec,M., Zakrzewska-Czerwinska,J.,
            Mendez,C., Botas,A., Moris,F., Cortes,J. and Gross,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-APR-2016) Pharmaceutical Biology, University of
            Tuebingen, Auf der Morgenstelle 8, Tuebingen 72076, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 30-JUL-2014
            Assembly Method                   :: CLC NGS Cell v. 7.0.4
            Genome Coverage                   :: 118.0x
            Sequencing Technology             :: Illumina HiSeq; PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/12/2024 01:56:15
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,832
            CDSs (total)                      :: 8,760
            Genes (coding)                    :: 8,562
            CDSs (with protein)               :: 8,562
            Genes (RNA)                       :: 72
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 198
            CDSs (without protein)            :: 198
            Pseudo Genes (ambiguous residues) :: 0 of 198
            Pseudo Genes (frameshifted)       :: 40 of 198
            Pseudo Genes (incomplete)         :: 174 of 198
            Pseudo Genes (internal stop)      :: 9 of 198
            Pseudo Genes (multiple problems)  :: 23 of 198
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 16:51:21
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 1477051
            Orig. end                         :: 1487444
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            KV411303.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10393
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="ectoine"
                     /region_number="6"
                     /rules="ectoine_synt"
                     /tool="antismash"
     cand_cluster    1..10393
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="ectoine_synt"
                     /kind="single"
                     /product="ectoine"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10393
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5393](+)"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5393
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
     misc_feature    complement(805..819)
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: weak, score: 17.8"
                     /tool="antismash"
     gene            1382..2092
                     /locus_tag="AWN90_RS06635"
                     /old_locus_tag="AWN90_06670"
     CDS             1382..2092
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1008: response
                     regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014351413.1"
                     /locus_tag="AWN90_RS06635"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AWN90_06670"
                     /product="response regulator transcription factor"
                     /protein_id="WP_067578598.1"
                     /transl_table=11
                     /translation="MTANLMIVEDDDRVRSALRLAMEDEGYDVAEAEEAEDALEHLRDN
                     GAPDVMIVDLMLGDMDGFSCIREIRRDHDVPIIVVSARDDTHDVVAALEAGADDFVTKP
                     FEIKEITARMRALRRRAQQFAEPPTPMEVVLDADPDAPLLLSPEGGTVRRGDEELHLTL
                     TEFRLLCELAETPGRVLSRSVLLERVWDRGFFGDERIVDVHVRRLRTKIERDPSEPAVV
                     VTVRGLGYRLDVRR"
     gene            2181..3107
                     /locus_tag="AWN90_RS06640"
                     /old_locus_tag="AWN90_06675"
     CDS             2181..3107
                     /GO_function="GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA]"
                     /GO_function="GO:0004673 - protein histidine kinase
                     activity [Evidence IEA]"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_process="GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1003: sensor
                     histidine kinase"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /locus_tag="AWN90_RS06640"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AWN90_06675"
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="WP_067578600.1"
                     /transl_table=11
                     /translation="MAVVAVVGRGVWETLLLGALCGTAIGAIFGLWLSRRLLEPLRQVT
                     GTAARIASGELGTRLPDTDDPDLEPTVAAFNTMVDSLQRRIDRERRLVGDVSHELRTPL
                     TTLTTSVGVMARYEDELPERSRRALALVQAELDHLRRLLDDLLALARAEARMHAADAEP
                     LSVPDLLTHTLAELRYPPDLLSTATGRDALVIGRKLELERAVVNLLDNAQRHGGGAVGV
                     GVERRGDEIIVTVDDAGPGVAAEDRERIFDRFVTVRRSRKSGTGTGIGLALVAETVAAH
                     GGTVSCTDRPGGGARFTVKLPGIPREK"
     misc_feature    2271..2273
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     misc_feature    complement(3131..3148)
                     /note="TFBS match to ArcA, Repressor of carbon oxidation,
                     confidence: weak, score: 15.09"
                     /tool="antismash"
     gene            3195..3749
                     /gene="ectA"
                     /locus_tag="AWN90_RS06645"
                     /old_locus_tag="AWN90_06680"
     CDS             3195..3749
                     /EC_number="2.3.1.178"
                     /GO_function="GO:0033816 - diaminobutyrate
                     acetyltransferase activity [Evidence IEA]"
                     /GO_process="GO:0019491 - ectoine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="ectA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019930017.1"
                     /locus_tag="AWN90_RS06645"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AWN90_06680"
                     /product="diaminobutyrate acetyltransferase"
                     /protein_id="WP_082871066.1"
                     /transl_table=11
                     /translation="MQTRTLPSPPSETTTIRTLRPGDGTALWRIAVDSQVLDANSSYAY
                     LLWCRDFAATSVVAEIGSDIAGFVTGYFRPQAPDTLFVWQVAVDHAYRGRGIAVSMLER
                     LVDSVAGQGISALETTVSPDNAASLAMFAALARRRGAELTRRQLFGPEDFPDGHEAEDL
                     YRIAPLHTVAQREEGIIDDHR"
     gene            3736..4998
                     /gene="ectB"
                     /locus_tag="AWN90_RS06650"
                     /old_locus_tag="AWN90_06685"
     CDS             3736..4998
                     /EC_number="2.6.1.76"
                     /GO_function="GO:0045303 - diaminobutyrate-2-oxoglutarate
                     transaminase activity [Evidence IEA]"
                     /NRPS_PKS="Domain: Aminotran_3 (27-359). E-value: 1.9e-75.
                     Score: 245.9. Matches aSDomain:
                     nrpspksdomains_AWN90_RS06650_Aminotran_3.1"
                     /codon_start=1
                     /gene="ectB"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_3"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1013: aminotransferase class-III"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019930018.1"
                     /locus_tag="AWN90_RS06650"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AWN90_06685"
                     /product="diaminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="WP_067578602.1"
                     /sec_met_domain="Aminotran_3 (E-value: 1.2e-76, bitscore:
                     256.0, seeds: 13, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTTAEMTVFESLESDVRGYCRSWPTVFDTARGSWITDAGGREYLD
                     FFAGAGALNYGHNNPVLKTALLDYIGRDGITHGLDMATVAKRELLETIEQVLLDPRGLD
                     YKVQFPGPTGANAVEAALKLARKVTGRTTILNFTNAFHGMTLGALSVTGNAAKRAGAGV
                     PLVHVNPMPYDGYLDGDEGLQWMARALDDHSSGVDKPAAVIVETVQGEGGVNVARPEWL
                     RQLSELCSARGILLIVDDVQMGCGRTGPFFSFEIAGITPDIVTLSKSIGGYGMPLALVL
                     MRRELDQWAPGEHNGTFRGNNPAFVTATVALDHYWRDDRLTLSTMAKGERIHAAFASLA
                     DTYEGVSTRGRGLVHGLVFEDASQAGKVSRIAFEQGLLVETSGAEDQVVKLLPPLTITD
                     DELDHGLSILGRAVETARGGN"
     aSDomain        3817..4812
                     /aSDomain="Aminotran_3"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_AWN90_RS06650_Aminotran_3.1"
                     /evalue="1.90E-75"
                     /label="AWN90_RS06650_Aminotran_3.1"
                     /locus_tag="AWN90_RS06650"
                     /protein_end="359"
                     /protein_start="27"
                     /score="245.9"
                     /tool="antismash"
                     /translation="DTARGSWITDAGGREYLDFFAGAGALNYGHNNPVLKTALLDYIGR
                     DGITHGLDMATVAKRELLETIEQVLLDPRGLDYKVQFPGPTGANAVEAALKLARKVTGR
                     TTILNFTNAFHGMTLGALSVTGNAAKRAGAGVPLVHVNPMPYDGYLDGDEGLQWMARAL
                     DDHSSGVDKPAAVIVETVQGEGGVNVARPEWLRQLSELCSARGILLIVDDVQMGCGRTG
                     PFFSFEIAGITPDIVTLSKSIGGYGMPLALVLMRRELDQWAPGEHNGTFRGNNPAFVTA
                     TVALDHYWRDDRLTLSTMAKGERIHAAFASLADTYEGVSTRGRGLVHGLVF"
     gene            5001..5393
                     /locus_tag="AWN90_RS06655"
                     /old_locus_tag="AWN90_06690"
     CDS             5001..5393
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     ectoine: ectoine_synt"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013126207.1"
                     /locus_tag="AWN90_RS06655"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AWN90_06690"
                     /product="ectoine synthase"
                     /protein_id="WP_067578604.1"
                     /sec_met_domain="ectoine_synt (E-value: 2.2e-52, bitscore:
                     174.3, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIVRTTAEITGTDRDVAAEGWRSKRIVLGGDGVGFSFHETTIQAG
                     TVHEFHYANHIEAVWLVEGEGTLTDLDNDVTYELAPGSMYLLDGHERHRLETRTQMRML
                     CVFNPPVTGKEVHDENGVYPLVAVGE"
     gene            5420..6301
                     /gene="thpD"
                     /locus_tag="AWN90_RS06660"
                     /old_locus_tag="AWN90_06695"
     CDS             5420..6301
                     /EC_number="1.14.11.55"
                     /codon_start=1
                     /gene="thpD"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PhyH"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019047359.1"
                     /locus_tag="AWN90_RS06660"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AWN90_06695"
                     /product="ectoine hydroxylase"
                     /protein_id="WP_067578606.1"
                     /sec_met_domain="PhyH (E-value: 1.8e-34, bitscore: 117.9,
                     seeds: 39, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTLADSRIDRYPTRTSAAAPPGERTDPTVWGAVTAPALASFDADG
                     YAIIDELLSTEEVAAFSAEIDRLAADPELRLDERVIIEKKSNRVRSIFEVHRISAAVAE
                     LVRDERVVGLARQVLGSEVYIHQSRVNYMPGFEGTGFYWHSDFETWHAEDGMPSPRACS
                     LSIALTDNYPFNGSLMVMPGSHRTFVPCQGETPADHYRESLREQQIGVPSHEHITELAD
                     RYGIAQFTGARGSALLFDSNIMHGSGNNITPFPRSNIFLVFNSVENTLSEPYSAPARRP
                     TYIASRDFDPVR"
     gene            complement(6378..7514)
                     /locus_tag="AWN90_RS06665"
                     /old_locus_tag="AWN90_06700"
     CDS             complement(6378..7514)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Acyl-CoA_dh_N"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Acyl-CoA_dh_M"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Acyl-CoA_dh_1"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1006: acyl-CoA dehydrogenase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006553521.1"
                     /locus_tag="AWN90_RS06665"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AWN90_06700"
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="WP_067578608.1"
                     /sec_met_domain="Acyl-CoA_dh_N (E-value: 3.4e-11, bitscore:
                     41.8, seeds: 790, tool: rule-based-clusters)"
                     /sec_met_domain="Acyl-CoA_dh_M (E-value: 1.5e-16, bitscore:
                     58.4, seeds: 43, tool: rule-based-clusters)"
                     /sec_met_domain="Acyl-CoA_dh_1 (E-value: 1.9e-23, bitscore:
                     81.3, seeds: 49, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MDFELTAEQQLLRDTVTEFLATRYDLEQSRAAARLGAGWQPEIWH
                     ALAAEVGILGAGLPDSAGGFGGGPVETMIIAEALGAALVVEPYVDTVVLGGGLLRRAGG
                     ERAASLLRGIVSGEVITAFAALEEDSGYDVRRVSTSARREGDSWVLDGAKTVVTTAPLA
                     THLLVTTRTADAAGLSLFVVAAGTPGLTLHPYRTIDDRSAADVLLDGVRVPADALLGTE
                     GGADDSLARAVDEAVAAIAAEAVGGMRRVLADTVDYAKQRRQFGVPIGSFQVLAHRLVD
                     MHLELEQSIAATYLVTLKLDADPTARARAAAAAKATIGRAARFIGQHAVQLHGAMGMTE
                     ELAIGHYFKRLTAIQYEWGTTDQHIARYAALQIGPAVR"
     misc_feature    7465..7477
                     /note="TFBS match to MatP, Cell cycle regulator,
                     confidence: medium, score: 23.79"
                     /tool="antismash"
     gene            complement(7518..8750)
                     /locus_tag="AWN90_RS06670"
                     /old_locus_tag="AWN90_06705"
     CDS             complement(7518..8750)
                     /GO_function="GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Acyl-CoA_dh_N"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Acyl-CoA_dh_M"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Acyl-CoA_dh_1"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1006: acyl-CoA dehydrogenase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011728213.1"
                     /locus_tag="AWN90_RS06670"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AWN90_06705"
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="WP_067578610.1"
                     /sec_met_domain="Acyl-CoA_dh_N (E-value: 7.3e-18, bitscore:
                     63.3, seeds: 790, tool: rule-based-clusters)"
                     /sec_met_domain="Acyl-CoA_dh_M (E-value: 3.1e-16, bitscore:
                     57.4, seeds: 43, tool: rule-based-clusters)"
                     /sec_met_domain="Acyl-CoA_dh_1 (E-value: 1.1e-20, bitscore:
                     72.4, seeds: 49, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MDLDWSPTDLAFRDEVRRFLDEHLTPDLRRAGRLATSVYPDHEAS
                     MRWQRILHARGWAAPAWPVQHGGCDWSPAQHYIFDRESTLAGAPALSPMGIRMVAHAII
                     AFGTAAQQAYFLPRILTGEVFFCQGYSEPEAGSDLAALTMAAVADGDDLVCTGSKIWTT
                     HATAANWIFCLVRTSRGERKQQGITFVLIDMTTSGIRVRPLVMTSGEQVQNQVFFDQVR
                     VPRANVLGRVDDGWTVAKYLLNFERGGAAYGPTLQVLAEGLAAQAATRPGPDGGALLDD
                     PAFAARLADARIRADVLEILEYRTMSAISRGRNPGPAASMLKILGTELSQRLTELALTA
                     AGPRGRVYQPHATMPGGPIAAYVPPADGYVSGEPWQAVAPLRYLNDRAGSIYAGSNEIQ
                     RNIIAKATLGL"
     gene            8957..9397
                     /locus_tag="AWN90_RS06675"
                     /old_locus_tag="AWN90_06710"
     CDS             8957..9397
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="AWN90_RS06675"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="AWN90_06710"
                     /product="hypothetical protein"
                     /protein_id="WP_156673894.1"
                     /transl_table=11
                     /translation="MFASRVSRSLLRAVPAVLLAVIAAATFTAVGGVATAAPMPAIPGI
                     PSVVHKLAFVPGQNTCGASGSIAGGQGDSYAISTAPGQHLAFDITSQNNNAAVSVAPLV
                     GPQIAAGVPHADIDDANGADYQIEVTSTDGNAADYTLTVTLS"
ORIGIN
        1 ggttcaggca gccgtgtgag gtgtcggtgt tgccctgcgc ccagacggtg gtgtcgagct
       61 ggtgcaggta gatgccgtcg gcgctgatgc gggtggcgta gtagatggcc tccttgtagc
      121 cgagccggga gttgaccggc aggccgtagg tggaggagtc catgatcacc ggattgccct
      181 tgtccatcac ggtgtagacg cccgagggag tgttgaagta gatggtcttc ccgccgacca
      241 cctcgctgcc gccgcggccc atggaggtgg gcatggtgcg gatcagcttg ccgttctcgt
      301 acacctggac ctgtttggtg ttgtcgtcgg cgatggacac gtgcgagggg ccgatgacga
      361 acgaggactt ggaatcctcc tggccgtaca ggcccgggcc cagttgcgcg ccatagatat
      421 tggcctgcac ggtcaccttc gtgcccggcg cccagtactg ctgcggccgc cagtgcgcat
      481 tgcggtcgtc gagccagtac cacgcgccct gcaccggcgg atcggtggtg accgtgagcc
      541 gcttctccgc ggcggcccgg tccgggatgt tctcgtcgaa gtgggcgacg atgaccgtgc
      601 cgacgccgta ggtgccgccg tcggccagcg gctgcccgcc ggtggtggtc agatacgcct
      661 tggtctgatt gctcggcgtg atggtggaga acgtggccgt ggtcgggccg ctcgggccgg
      721 agatcgtcac gccctcggcc ttgaccgtgt aggtgtggtc gtagccgagc ggaccggacg
      781 gtttccatgc ggtcttgtcc ggcgtgaaga tgccgtccac gggtttgccc tgctcattgg
      841 tcacgctcac cgcggtcagg accccatcgg acgcgctcac ctcgatcttg cccagcgggt
      901 cgacgttctt gctgccgtcc ccgggcgtca ccgagatgat cggtgccgcc ggacccggtt
      961 tcgccggcgt cgaattcgac gacgagtccg gatgggatga gcacgcggcc atcagcatca
     1021 cgaccagggc ggaccccagt gcgataccga atcggcagcg gcgacctgac atggtgtctc
     1081 ctgcgctcgc cgtgcgcctg ggcggacggg cccccgcagc gcaccaatcg cctaactgtc
     1141 ttgacgggta gtttacgggt cgcccgcgcg gcccggcact cgaggtgttg ccccaggttt
     1201 acgtggtggc cagcggggta ctcccactac cgatgtatgt cccgagagcg gttcgaccgc
     1261 tgctgccgat agcggtgcgt aaggatgcat gagctacggg cctcggtctg caagggtgga
     1321 gagctatgag ggagcgcagc gaccgattga tcgacacagc cagcgccggt gaggcggcgg
     1381 catgaccgca aacctgatga tcgtggagga cgacgaccgg gtacgcagtg ccctgcggtt
     1441 ggcaatggag gacgagggct acgacgtcgc cgaggccgag gaggccgagg acgcgctcga
     1501 gcatctgcgc gacaacgggg cgccggatgt gatgatcgtc gacctcatgc tgggagatat
     1561 ggacggcttc tcctgcatcc gggagattcg ccgcgatcac gatgtgccga tcatcgtggt
     1621 gagcgcgcgc gacgacaccc atgacgtggt ggcggcgctg gaggcggggg ccgacgactt
     1681 cgtcaccaag ccgttcgaga tcaaggagat caccgcccgc atgcgcgcgc tgcgccggcg
     1741 cgcccagcag ttcgccgagc cacccacccc gatggaggtg gtgctcgacg ccgatcccga
     1801 tgcgcccctg ctgctttcgc cggagggcgg caccgtgcgg cgcggcgacg aggaactgca
     1861 cctgacgctc accgaattcc ggctgctgtg cgagctggcg gagaccccgg gccgggtgct
     1921 gtcgcgcagc gtgctgctgg aaagagtctg ggaccgcggc tttttcggcg acgagcggat
     1981 cgtggacgtg catgtgcgcc ggttgcgcac caagatcgag cgggatccgt ccgagcccgc
     2041 cgtcgtcgtg acggtgcgcg ggctcggcta ccgactcgat gttcggcgat aggcgtcgtc
     2101 gcctgcctac acgccaaagt ctgcgtggcc gtgcggttgt cgcgttcgcc gccaccgccg
     2161 gtctggtttc cgcactgctc gtggccgtcg tcgccgtcgt cggccgcggc gtgtgggaga
     2221 cgctgctgct gggcgcgctg tgcgggaccg cgatcggcgc gatcttcgga ttatggctca
     2281 gtagaaggct tctggagccg ttgcgtcagg tgaccggcac ggccgcacga atcgcgtcgg
     2341 gcgaattggg cacgcggcta ccggataccg acgatccgga cctggaaccg actgtggcgg
     2401 cgttcaatac gatggtcgac tcgttgcagc ggcgcatcga tcgcgagcgg cggctggtcg
     2461 gcgacgtcag ccacgagctg cgtaccccgc tgaccaccct caccaccagc gtcggtgtca
     2521 tggcccgtta cgaggacgag ttgcccgagc gctcccgccg ggcattggcc ctggtacagg
     2581 ccgaactcga tcatctgcgt cgtttgctgg acgatttgct ggccctggcc cgcgccgagg
     2641 cgcgcatgca cgccgccgac gcggaaccgc tgtcggtccc cgatctgctc acccacaccc
     2701 tggccgagct gcggtacccg cccgacctgc tgtcgacggc cacgggccgc gacgccctgg
     2761 tcatcgggcg caaattggag ctggagcgcg cggtggtgaa cctgctggac aacgcccagc
     2821 gacacggcgg cggcgcggtc ggggtcggtg tcgaacgccg cggcgacgag atcatcgtca
     2881 cagtcgacga cgccggaccc ggtgtcgcag ccgaggatcg ggagcggatc ttcgaccgtt
     2941 tcgtcacggt tcggcgcagc cgaaaatcgg gcaccggcac cggaatcggc ctggccctgg
     3001 tggcggagac cgtcgccgcg cacggcggaa cggtgtcgtg cacggaccgg cccggcggcg
     3061 gcgcgcgctt cacggtgaag ctacccggta taccccggga aaagtgaggt aactcaccaa
     3121 aaacgcctct gtaacacgac cgtaaagtag ccgatcggtt tgcctcaaag ttcgcgggca
     3181 accctggaat ggtgatgcag acccgaacgc tgccttctcc tccctccgag accacgacca
     3241 tccgcaccct gcgccccggc gacgggaccg cgctgtggcg gatcgccgtg gactcgcagg
     3301 tgttggacgc gaattcgagc tacgcgtacc tgctctggtg tcgtgacttc gcggccacct
     3361 cggtggtcgc cgaaatcggt tccgacatcg cgggtttcgt gacgggttac ttccgcccgc
     3421 aggcgccgga cacgttgttc gtctggcagg tcgccgtcga tcacgcctac cgcggcaggg
     3481 gaatcgccgt ctcgatgctg gagcgtctgg tcgacagcgt tgccgggcag gggatttcgg
     3541 ccctggagac cacggtctcg ccggacaacg cggcgtcgct ggcgatgttc gccgcgctgg
     3601 cgcgccgccg cggtgcggaa ctcaccagga ggcagctgtt cggccccgag gattttccgg
     3661 acgggcacga agccgaagac ctgtaccgga tcgcgccact gcacacagtg gcgcagagag
     3721 aggaagggat catcgatgac caccgctgag atgaccgttt tcgaatcatt ggaatcggac
     3781 gtacgcggtt actgccggtc ctggccgaca gtgttcgaca ccgcccgagg ctcctggatc
     3841 acggacgcgg gcggacgcga gtacctggac ttcttcgccg gcgccggtgc gctgaactac
     3901 ggccacaaca atccggtgct caagacggcc ctgctggatt acattgggcg cgacggcatt
     3961 acgcacggcc tggacatggc caccgtcgcc aagcgggagc tgctggaaac catcgagcag
     4021 gtcctgctcg atccgcgcgg actcgattac aaggtgcagt tccccggacc gaccggcgcg
     4081 aacgccgtcg aggccgcgct caagctggcg cgcaaggtga ccggccgcac cacgattctc
     4141 aacttcacca acgccttcca cggcatgacg ctgggcgcgc tgtcggtcac cggcaacgcg
     4201 gctaagcgcg ccggcgcggg cgtgccgctg gtgcacgtga atcccatgcc ctacgacggc
     4261 tacctggacg gcgacgaggg gctgcagtgg atggcgcggg ccctcgacga ccactcctcg
     4321 ggcgtggaca agcccgcggc ggtgatcgtg gagaccgtgc agggcgaggg cggcgtcaat
     4381 gtggcgcggc cggagtggct gcggcagctg tccgaattgt gctcggcgcg tgggattctg
     4441 ctgatcgtcg acgatgtgca gatgggttgc gggcgtaccg gcccgttctt ctccttcgag
     4501 atcgccggta tcacaccgga tatcgtgacg ctgtcgaagt cgatcggcgg ctacggcatg
     4561 ccgctggcgc tggtgctgat gcgccgggag ctggaccagt gggcgccggg tgagcacaac
     4621 ggcaccttcc ggggcaacaa ccccgcgttc gtcaccgcca cggtggcgct ggatcactac
     4681 tggcgcgacg atcgcctgac gttgtcgacg atggccaagg gtgagcgaat ccatgccgcg
     4741 ttcgcgtcgt tggcggacac ttatgagggg gtgtccaccc gcgggcgcgg cctggtgcac
     4801 gggctggtgt tcgaggacgc gtcgcaggcc gggaaggtga gcaggatcgc cttcgagcag
     4861 gggctgctgg tggagacctc cggcgccgag gatcaggtgg tgaagctgct cccgccgctg
     4921 acgattaccg acgacgaact ggaccacggc ctgagcatcc tgggccgggc ggtcgagacg
     4981 gccagaggag gcaactgaca atgatcgtgc ggaccaccgc ggagatcacc ggcaccgacc
     5041 gcgacgtggc ggccgagggt tggcgcagca agcggatcgt gctcggcggc gacggcgtgg
     5101 gattctcctt ccacgagacc acgatccagg ccgggacggt gcacgagttc cactacgcca
     5161 accacatcga ggcggtgtgg ctggtcgagg gtgagggcac gctcaccgac ctcgacaacg
     5221 acgtgaccta cgagctggcc ccgggctcga tgtatctgct ggacgggcac gagcgccacc
     5281 gcctcgagac ccgcacgcag atgcggatgc tgtgcgtgtt caacccgccg gtgaccggca
     5341 aggaggtcca cgacgagaac ggggtctacc ccttggtcgc ggtgggcgag taagttcggg
     5401 tgaggaggag aacactgcaa tgacgttggc cgacagtcga atagatcgct acccgacgcg
     5461 cacctcggcg gccgctccgc cgggcgagcg gaccgatccc acggtgtggg gtgcggtgac
     5521 cgcgccggcg ctggcgagct tcgacgccga cggatacgcg atcatcgacg agttgctgtc
     5581 cacggaggag gtggccgcct tctccgccga gatcgaccgg ctggcggccg atcccgagct
     5641 gcgcctggac gagcgggtga tcatcgagaa gaagtcgaac cgggtgcggt cgatcttcga
     5701 ggtgcaccgg atcagcgccg cggtggcgga gttggtgcgc gacgagcggg tggtcgggct
     5761 ggcccggcag gtgctcggct cggaggtgta catccatcag agccgggtga actacatgcc
     5821 gggcttcgag ggcaccgggt tctattggca ctcggacttc gagacctggc atgcggagga
     5881 cgggatgccg tcgccgcggg cgtgcagtct gtcgatcgcg ctgacggata attatccgtt
     5941 caacgggagc ctgatggtga tgcccggttc gcatcggacg ttcgtgccgt gtcaggggga
     6001 gaccccggcc gaccattatc gggagtcgtt gcgcgagcag cagatcgggg tgccgtcgca
     6061 cgagcacatc accgagctgg ccgatcggta cggtatcgcg cagttcaccg gggcgcgcgg
     6121 gtcggcgctg ctgttcgact cgaatatcat gcacggctcc gggaacaaca tcaccccgtt
     6181 cccgcggtcg aacatcttcc tggtgttcaa cagcgtcgag aacacgctga gcgagccgta
     6241 ttcggcgccc gcgcggcgcc cgacctacat cgcgagccgg gacttcgacc cggtccgcta
     6301 gaaacccacg cccggcctcg ccgactcagc gggacgacac taccgggcgt aggcgatcgg
     6361 cccggccgtc cgaggcgtca ccggacggcc gggccgatct gtagggccgc gtaccgcgcg
     6421 atgtgctggt cggtcgtgcc ccactcgtac tgaatggcgg tgagccgctt gaagtagtgg
     6481 ccgatggcca gttcctcggt catgcccatg gcgccgtgca gctggaccgc gtgctggccg
     6541 atgaagcggg cggcgcggcc gatggtggcc ttggcggcgg ccgccgcgcg agcgcgggcg
     6601 gtcgggtcgg cgtcgagctt cagcgtgacc agatacgttg cggcaatgga ctgttcgagc
     6661 tccaggtgca tatcgaccag gcggtgcgcc agcacctgga agctgccgat cggcacgccg
     6721 aactggcggc gctgcttggc gtagtcgacg gtgtcggcga ggacgcggcg catgccgccg
     6781 accgcctcgg ccgctatcgc cgccaccgcc tcgtcgacgg cgcgcgccag cgaatcatcc
     6841 gcgccgcctt cggttcccag cagcgcatcg gccgggacgc gcaccccgtc gagcagcacg
     6901 tcggcggcgc tgcggtcgtc gatggtgcgg taggggtgca gggtgagccc gggggtaccg
     6961 gccgccacca cgaacagcga cagccccgcg gcgtcggcgg tgcgcgtggt gacgagcagg
     7021 tgggtcgcca gcggcgcggt ggtcaccacc gtcttcgcgc cgtcgagcac ccacgaatcg
     7081 ccctcgcgcc gagccgatgt cgacacccgc cgcacgtcgt agcccgaatc ctcctccagc
     7141 gccgcgaatg ccgtgatcac ctcgccggaa acgatgccgc gcaacagcga tgcggcgcgc
     7201 tcgcccccgg cgcggcgcag cagcccgccg ccgagcacca cggtgtccac gtacggctcc
     7261 accaccagcg ccgcgcccag cgcctcggcg atgatcatcg tctcgaccgg tccgccgccg
     7321 aatccgcccg cgctgtcggg caatccggcg ccgaggatgc cgacctcggc ggcgagggcg
     7381 tgccagatct ccggctgcca tccggcgccc aggcgggccg cggccctgct ctgctccagg
     7441 tcgtagcggg tcgccagaaa ctcggtgacg gtgtcacgga gcagttgctg ttcggcggtg
     7501 agttcgaagt ccatcggtca cagtcccagg gttgccttgg cgatgatgtt gcgctgaatc
     7561 tcgttgctac cggcgtagat cgagccggcc cggtcgttga ggtaccgcag cggcgccacc
     7621 gcctgccacg gttcgccgct gacgtatccg tcggcgggcg gcacatacgc ggcgatcggc
     7681 ccgccgggca tggtggcgtg cggctgatac acccgcccgc gcggcccggc ggccgtgagc
     7741 gccaattcgg tgagccgctg gctcagttcg gtgcccagga tcttcagcat cgaggccgcg
     7801 gggcccggat tccggccgcg cgagatcgcc gacatggtcc ggtattccag gatctccagc
     7861 acgtccgcgc ggatgcgggc gtcggcgagc cgggccgcga aggccggatc gtccagcagc
     7921 gcaccgccgt ccggcccggg ccgggtcgcg gcctgggcgg ccagcccctc ggccagcacc
     7981 tgcagcgtgg ggccgtaggc cgcgccgccg cgctcgaagt tcagcagata tttggccacg
     8041 gtccagccgt cgtcgacgcg gcccagcacg ttcgcccgcg gcacccggac ctggtcgaag
     8101 aagacctggt tctgcacctg ctcgccggag gtcatcacca gcgggcgcac ccgaatgccc
     8161 gacgtggtca tgtcgatgag cacgaaggtg atgccctgct gtttgcgctc gccgcgggag
     8221 gtgcggacca ggcagaagat ccaattcgcc gccgtcgcgt gggtggtcca gatcttgctg
     8281 cccgtgcaca ccagatcgtc gccgtcggcc acggccgcca tggtcagcgc ggccaggtcc
     8341 gacccggcct ccggctcgga gtagccctgg cagaagaaga cctcgccggt gaggatgcgc
     8401 ggcaggaagt atgcctgctg cgccgcggtc ccgaacgcga tgatcgcgtg cgccaccatc
     8461 cgaataccca tgggggacag ggccggtgcg cccgcgagcg tggattcgcg gtcgaagatg
     8521 tagtgctggg cggggctcca gtcgcagccg ccgtgctgca ccggccaggc cggggccgcc
     8581 cagccgcgcg cgtgcaggat gcgctgccac cgcatgctgg cctcgtggtc ggggtagacg
     8641 ctggtggcga gccgcccggc gcggcggagg tctggggtca ggtgctcgtc caggaagcgc
     8701 cgcacctcgt cgcgaaacgc cagatcggtg ggtgaccagt ccagatccac gggcaactcc
     8761 ttgtgcgata aatgttgccc taccttctca tttactcacg cccgagcgca aatggtcgtt
     8821 aacttaatgg ctcgcgacct cccactgtgg gctgagggtg ggtgcgggtg ttcggcgcgg
     8881 cgattccggc gtgtcgagca cgaatttccc gccgtagctt gcgatcttga cggcgatgtg
     8941 gggaaagatg ttcggcatgt tcgcatctcg agtgtcccgt tcgctgttgc gtgccgtccc
     9001 cgcggtgctc ctcgccgtca ttgccgcagc caccttcacc gcggtcggcg gggtcgccac
     9061 cgccgcgccg atgcccgcaa tccccggcat cccgagcgtc gtgcacaagc tggcgttcgt
     9121 gcccggccag aacacctgcg gcgccagcgg cagcatcgcc ggcggccagg gtgacagcta
     9181 cgcgatctcc accgcgcccg ggcagcacct ggccttcgac atcacctccc agaacaacaa
     9241 cgccgccgtg tcggtggccc cgctggtcgg cccgcagatc gccgccgggg tgccgcacgc
     9301 cgacatcgac gacgccaacg gcgccgacta ccagatcgag gtgacctcca ccgacggcaa
     9361 cgccgccgac tacaccctga ccgtcaccct gtcctgattc ccgctcccgc atccgagcag
     9421 ccgggcgaac atattcgccc ggcatactcg gagcaacggg gctggacacg gtagcctggc
     9481 gtgctgtgca cctgaagagt ctgacgctga agggcttcaa atccttcgcg tccgcgacga
     9541 ccctgcgctt cgagccgggc atcacctgcg tggtgggccc caacggctcc ggcaagtcga
     9601 acgtcgtcga cgcgctcagc tgggtgatgg gtgaacaggg cgcgaaggcc ctgcgcggcg
     9661 ggaagatgca ggacgtcatc ttcgccggca cctccggccg cgccccgctg ggccgcgccg
     9721 aggtcacgct gaccatcgac aactccgacg gcgcactgcc catcgaatac tccgaggtct
     9781 ccatcacccg gcgcatgttc cgcgacggcg ccggcgaata cgagatcaac ggcagctcct
     9841 gccgcctgat ggacgtgcag gaactgctca gcgactccgg catcggccgg gagatgcacg
     9901 tcatcgtcgg tcaggggcag ctgtcggcga tcctggaatc gcgtcccgag gaccgccgcg
     9961 ccttcatcga ggaggcggcc ggtgtgctca agcatcgccg tcgcaaggag aaggcggtcc
    10021 gcaagctgga ggccatgcag gccaacctgg cccgcctcac cgacctcacc accgaactgc
    10081 gccgccagct caagccgctg ggccggcagg ccgaggtcgc ccgccgcgcc gccaccgtgc
    10141 aggccgatct gcgcgacgcc cggctgcgcc tggccgccga cgatctggtc acccgccgcc
    10201 gcgagctgga gagccagcag agcaaggaag ccttcgcccg cgagcagcag atcaacgtcc
    10261 agaccgagct cgacgccgcc aatgccgcac tggcgcagca ggaattccag ctctcccggc
    10321 tgacgccggg cgccgaggcc gccgcgcaga cctggttcca gctctcggcg ctggtggagc
    10381 gcgtcaatgc cac
//
