LOCUS       NZ_JTKO01000010        11614 bp    DNA     linear   CON 11-APR-2024
DEFINITION  Staphylococcus pseudintermedius strain 2001-08-299-1 contig_10,
            whole genome shotgun sequence.
ACCESSION   NZ_JTKO01000010
VERSION     NZ_JTKO01000010.1
KEYWORDS    WGS; RefSeq.
SOURCE      Staphylococcus pseudintermedius
  ORGANISM  Staphylococcus pseudintermedius
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus; Staphylococcus intermedius group.
REFERENCE   1  (bases 1 to 269712)
  AUTHORS   de Vries,L.E., Hasman,H., Jurado Rabadan,S. and Agerso,Y.
  TITLE     Sequence-Based Characterization of Tn5801-Like Genomic Islands in
            Tetracycline-Resistant Staphylococcus pseudintermedius and Other
            Gram-positive Bacteria from Humans and Animals
  JOURNAL   Front Microbiol 7, 576 (2016)
   PUBMED   27199912
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 269712)
  AUTHORS   de Vries,L.E. and Agersoe,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-DEC-2014) Department of Technology, University College
            Metropolitan, Sigurdsgade 26, Copenhagen 2200, Denmark
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: SEP-2014
            Assembly Method                   :: CLC NGS Cell v. 7.5.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 20.0x
            Sequencing Technology             :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_001622945.1-RS_2024_04_11
            Annotation Date                   :: 04/11/2024 12:27:59
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,368
            CDSs (total)                      :: 2,296
            Genes (coding)                    :: 2,259
            CDSs (with protein)               :: 2,259
            Genes (RNA)                       :: 72
            rRNAs                             :: 5, 2, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 5, 1 (5S, 16S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 57
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 13 of 37
            Pseudo Genes (incomplete)         :: 26 of 37
            Pseudo Genes (internal stop)      :: 7 of 37
            Pseudo Genes (multiple problems)  :: 9 of 37
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 08:20:57
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 246960
            Orig. end                         :: 258574
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            JTKO01000010.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..11614
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="RiPP-like"
                     /region_number="3"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..11614
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..11614
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="True"
                     /core_location="[5000:6614](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..6614
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            2581..3711
                     /locus_tag="NO87_RS09955"
                     /old_locus_tag="NO87_09955"
     CDS             2581..3711
                     /EC_number="2.4.-.-"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Glycos_transf_2"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /locus_tag="NO87_RS09955"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NO87_09955"
                     /product="glycosyltransferase"
                     /protein_id="WP_063284912.1"
                     /sec_met_domain="Glycos_transf_2 (E-value: 6.6e-20,
                     bitscore: 65.7, seeds: 256, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIEKINKLEIISEYTTISVVYIVKNERDYIIKSLLSILDLADEII
                     IVDTGSEDNTLDLIKKMCDKKIKIFTFNWCDDFSKARNFANAKATCDWIMILDADEIVR
                     KNDNLKFYLTYLRIFDDFENTAFNFKVIRDEEVYKTSKLIRNTNTLTYKGRVHETFFSL
                     NNSVSYANLNVEVLGIRRGSQKKTNYYNKLLLKTIIDFPKEGRWYYLYLRDNIEDINYN
                     KMLKFCKKIIFKRDAISGIEINNNYYSVRIIFLLMFKLLEESNHERFTYYMELLEKDFN
                     HTQDFIFLKYSYYIKKLNIDATNLLKSFLENYDLLNEGDVIFSKECTHSLLGASLFWGG
                     YLKESKKVFEELEINSKDTVCFYNKKFIYDCLSTLS"
     gene            3786..4094
                     /locus_tag="NO87_RS09960"
                     /old_locus_tag="NO87_09960"
     CDS             3786..4094
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF024424.4"
                     /locus_tag="NO87_RS09960"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NO87_09960"
                     /product="DUF3884 family protein"
                     /protein_id="WP_063284913.1"
                     /transl_table=11
                     /translation="MIKISLKKILCQLSQKKLYEKTFQSIYIVDFSLLDRAPLFKDEFK
                     VIGTWYSYSGKRWICHTELSTEQFKKMITKNIDHKDLEKVKFYLDYLPFSITNEIPF"
     misc_feature    complement(4248..4263)
                     /note="TFBS match to CopR, Copper-responsive regulator,
                     confidence: weak, score: 24.05"
                     /tool="antismash"
     misc_feature    complement(4277..4290)
                     /note="TFBS match to CatR, H2O2-responsive repressor,
                     confidence: strong, score: 21.85"
                     /tool="antismash"
     gene            4469..5044
                     /locus_tag="NO87_RS12470"
     CDS             4469..5044
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Peptidase_C39"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF015377.4"
                     /locus_tag="NO87_RS12470"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="cysteine peptidase family C39 domain-containing
                     protein"
                     /protein_id="WP_187530240.1"
                     /sec_met_domain="Peptidase_C39 (E-value: 1.1e-41, bitscore:
                     135.8, seeds: 36, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKFSKRFYRAQVDSQDCGAAALAMILEFYGSHYSLDFLRRKLRTT
                     VNGTTAYGLVQVADKLGFETVPIKADISLFEIDDLQYPFIVHVKKNNNHFHYYVVIRCD
                     KKYIYIADPVVNLTKMPYREFKEEWTGVTILLKPTSLYKPIKQDKVRLTYFIPLLLKEK
                     KIDYKNNNHFHFSQYDKYFRFIFYEVNY"
     gene            5001..6614
                     /locus_tag="NO87_RS09965"
                     /old_locus_tag="NO87_09965"
     CDS             5001..6614
                     /GO_component="GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /GO_function="GO:0043214 - ABC-type bacteriocin transporter
                     activity [Evidence IEA]"
                     /GO_process="GO:0043213 - bacteriocin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: TIGR01193"
                     /gene_functions="transport (smcogs) SMCOG1288: ABC
                     transporter related protein"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:TIGR01193.1"
                     /locus_tag="NO87_RS09965"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NO87_09965"
                     /product="peptide cleavage/export ABC transporter"
                     /protein_id="WP_272914040.1"
                     /sec_met_domain="TIGR01193 (E-value: 1.5e-204, bitscore:
                     675.4, seeds: 5, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MINILGSYFMKSIIDFYIPQKLKTWVSMISMALIASYIMQQILSY
                     LQKYFLILLVKTYSINILSPYIKHLFRLPITFFETGRAGDILSRLNDANSITDALASTM
                     LSLFLDVTIIVGISIFLYYQSTILFVLILIIIPIYAVIVFGFMNSFERKRIKSMEANAT
                     LSSYLMENITGMETIKALTCEKKCYKQIKYNFSNYCEKTFLLNKLNIKQNVLKKVVQLI
                     LNVSILWVGANLVIDNQISIGTLLVFNSLMIYFMNPIENIMNLQLKLQIAGVANNRLNE
                     IFTVKSEFEKNKLDIKLNQNSSKLIFSNISYGYVYEKEILTDINITIEHNSKVAFIGES
                     GSGKTTLAKLMVSFFEPSKGKITLGGLDITNINKSVLRQQIIYIPQQTYIFNGTILENL
                     LLGVVKEVTQKDIDSAIKMVELKSFIDSLPLGLDSVLSSDFSALSGGQRQKIALARALL
                     TNSSILIMDEATSNIDILTERKIINNLMALNKTIIFISHNLNITQKVDKIFVLAKGKII
                     EEGSHEFLLKAKGYYSNLIN"
     gene            complement(6871..7686)
                     /locus_tag="NO87_RS09970"
                     /old_locus_tag="NO87_09970"
     CDS             complement(6871..7686)
                     /GO_function="GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_process="GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019168536.1"
                     /locus_tag="NO87_RS09970"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NO87_09970"
                     /product="ZIP family metal transporter"
                     /protein_id="WP_015728742.1"
                     /transl_table=11
                     /translation="MLDYISNMPPFLQALLAGVITWLLTALGAATVFVFKSVNEKVLNS
                     MQGFAAGIMIAASFWSLLQPAIESSSGSTVPWLPAAIGFLLGGVFIRSLDYVIPHMHRN
                     ATDESQHQEGVETSLNKNTLLLLAITLHNIPEGLAIGVAFGGIATGNEHATLLGALGLA
                     IGIGIQNIPEGAALSLPIHASGQSKWKAFNYGQASALVEPVFATVGAAAVLVVTPILPY
                     ALAFAAGAMIFVVVEELIPDSQAGKNTDLATLSLMVGFTIMMILDVALG"
     gene            7798..9675
                     /locus_tag="NO87_RS09975"
                     /old_locus_tag="NO87_09975"
     CDS             7798..9675
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019166146.1"
                     /locus_tag="NO87_RS09975"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NO87_09975"
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="WP_063284914.1"
                     /transl_table=11
                     /translation="MEAYKIERLNKSYADKVIFDDLQLSISNQERIGLVGINGTGKSSL
                     LKVIAGMDEDFDAVQSHPKQYRIRYAAQNHNLEPTLTVYEAVYSADSKELQVIKRYEEA
                     TAQYAEQQTDKAFDAMMAAQADMDAYSAWDYSAEIKTILSKLGIHDTTRRMSELSGGQQ
                     KRVVLARTLIEKPDLLLLDEPTNHLDFESIHWLIQFVKSYPKTVVFVTHDRYFLNEVAT
                     RIVELRNGKLDTYPGNYEAYIAMRAEREEIEVRQQTKQRALFKQELAWMRAGVKARTTK
                     QQARQDRFHDLKEQVQSHQVQGKGSLNLAYSRLGKQVFELENVTKRIDGRTLFENVSTL
                     IQKGVQIGIVGPNGVGKTTMLNILAGEDQHFEGVLKIGQTVKVAYFKQTDERLNRDVRV
                     IDFLREESELARQKDGTTVSITQLLERFLFPSVTHGKKVYKLSGGEQKRLYLLKLLVHQ
                     PNVLLLDEPTNDLDTETLTILEDYIQTFGGTVITVSHDRYFLDKVVDCYWYIHDGQIDM
                     ILGQFEDYLAYQKQLEQATTQVASVKQQPTLQPKKKKGLSYNEKREYDMLLVRIEETES
                     RLAEIDAEMIEANADYAKIKALNEEKETLEAQYDTDMTRWSELEEILEQ"
     gene            9685..11466
                     /gene="recQ"
                     /locus_tag="NO87_RS09980"
                     /old_locus_tag="NO87_09980"
     CDS             9685..11466
                     /EC_number="3.6.4.12"
                     /GO_function="GO:0003678 - DNA helicase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /gene="recQ"
                     /gene_functions="other (smcogs) SMCOG1122: ATP-dependent
                     RNA helicase"
                     /gene_kind="other"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016424488.1"
                     /locus_tag="NO87_RS09980"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="NO87_09980"
                     /product="DNA helicase RecQ"
                     /protein_id="WP_063284915.1"
                     /transl_table=11
                     /translation="MEQTLSHYFGYKSFRPGQKEIIERVLNHHHTLGVLPTGGGKSLCY
                     QVPGLMFKGTTIVISPLISLMKDQVDQLTAMGISAAYLNSSLSTQAQKEIETQLVNGEL
                     KFLYIAPERFDQTYFEMLLRRVDISLVAFDEAHCISKWGHDFRPSYQAVIQRVLTMPQQ
                     FSIVALTATATTEVQQDIMERLMISRQHLVKTSIRRRNLKFQVNGTYQRQKFVLDYIQK
                     HSQKAGIVYCSTRKQVEALYEALEDADIPVSYYHAGMTAKQRDEAQNDFLYDRTRVVVA
                     TNAFGMGIDKSNVRYVIHYNMPGDLESYYQEAGRAGRDGLDSDCILLYSDRDIALHQYF
                     IGASKADDDYKERMGEKLTKMIQYTKTTKCLEATLVHYFEPNEKLEECHQCSNCIDKNK
                     TYNMTKEAKMIISGVARLRQQEGYQTVIQVLRGEVSDYIRHQGYENLSTYGLMKDYTTG
                     ELHHLIDELRFKGFLNEHDEILMCDASVEKLLSEDTQVFTRPFKRKSKETVHINTVEGV
                     DRTLFEKLVEVRREMSERLNMPPTNIFTDYTLEAFAKRKPVTKQEMIQIDGVGSYKLKH
                     YCPEFLEAIQEYKMHRS"
ORIGIN
        1 ttggtatcga taagttttat gatgcaacat attttgacat gtctccagaa aacttagaaa
       61 accttggatt aaaagataaa gagtttttca aagaggcggc ggattactta tctaaagaaa
      121 aacaaccgtt ctattcacat ttaattacgt tgacgaacca ttatcctttt acattaagtg
      181 atgaagacgc aacgattgca aaaggtgaca caggtaactc aacggtcgat ggttacattc
      241 aaacagcgcg ttacttagat gagtcgttag aacagttcat taatgacttg aaagagcgtg
      301 gtctgtacga tgattcagtc attatgattt acggtgacca ttatggtatc tcagaaaatc
      361 ataacaaagc gatgtcacag ttattaggtg aagagattac accagcgaag tttaatgact
      421 tgaatgcaac gggtttctgg ttaaaagcac caggggttga gcctaaagtg gacgacacgt
      481 atgcaggtca agttgatgtg atgccaacat tacttcactt gttaggtatt gatacgaaaa
      541 attatctcat gtttggtaca gatatgttat ccaaggacca taaagaaatt gtaccattcc
      601 gtaatggtga tttcgtgacg agaaattata aatatgtcaa cggtaaagcc tacagtaaca
      661 gagacaatca accgttagaa acaccgccag cagatcttga taaggacaaa aagcaggtac
      721 aacatgactt agaaatgagt gataaagtct tgaatggaga cttattccga ttctataaaa
      781 atcctgattt caacaaattg aacccagcag attataaata tccgacaggt ccaaaggcaa
      841 atcaaaaata aaatgttatg acagggaagt gtacaaatca gtgcatttcc ctgtttttta
      901 ggttgtatat atttggttgt gtatttttaa cggttggtga gagatttact gcccgttttt
      961 taattttgaa agacctgttt atttaaattt gaagaagcat cgtttttact ttaaagcttg
     1021 tcaacaaacc tttataacta aaatacccta tattcaaccc cgaagtctca aataaggtca
     1081 aacgcttgat gacgcggaag ctaactaaat tcacatctga aaaagatgtc gctaaaagtc
     1141 tatgtgtatc accttcaact gttcattgcc atttaaaaga ggtgagtaat ttgtaaagac
     1201 atacgcacat gatgctttac ctgagcactt agcttttgac gaattcaaat caatgaaaga
     1261 tatcgaagga ccaatgagct ttatttactg tgatagtgta acccatgata tcatcgacat
     1321 tttacctaat cgttgcaaac attcatcaaa tatttgccct gaacctcaaa tactatgcat
     1381 catcctcatt taacaaatgg cccgattgaa aggattaata ataaaacaaa tgtgtgtctt
     1441 atagttatag aaatttctgg aatcttaatc atttctaaga tacttgtaag tgcatataaa
     1501 aaacggaatg ggtcttaaaa atttttgatg ctattgttgt aatattttta ttaaacttgt
     1561 ccgcattacg atttgtggat ttaattgtca tcatggtttc gctttcctag agccggccct
     1621 tcaactcaat tcggcttgat tgaagccaaa ttggatttta ggagcagtac agaaacctca
     1681 tatgcaacaa gatttcgttg tactgccttg catggctgat tagacttctc gaaagtgaaa
     1741 acactgagaa gtcagcccgc tactgcgata aacgcctccc accattaaag taaagaggcg
     1801 actgtcaaca gtatgcacgt tccccctcag gcgtctcagc catctgggta atcctatatc
     1861 aatacagcga aactgagtgc aagattatga aatccagaaa acaatggcat cattttttat
     1921 acacattcac cccttatttt cgaatgatca atcattatta atcgatatca gtctataaat
     1981 agaagcagag attaggaaac cataatgtct cagtgccgtt attgtctcac caaccgacgt
     2041 tgacaaagaa cccgtctcaa tcgcctctat cgttggaaag tattttagat taaaacattt
     2101 cctttcataa aaagaataaa cttgtccata tttatcttgg cgtttatata tgaaatgcag
     2161 cattttcatt tcgccatttt cctacatttt ataaccgtca tctacactgc atgtacttct
     2221 gtaattaaag tatatgtgag aaattatact tccgaatata aataaatatt gacactatat
     2281 ttacaaagta atacaattat ttataattaa agcatagcaa tttgtaaatg tgatagtaga
     2341 attacgtatt tatcataata attcgcctcc cagtcttgca aaacttttaa aaatattttg
     2401 ttttattatt tttttttttg agggggtgtg attaactttg aaaaacatat accctatatt
     2461 tgatgaagat tggttagaaa aaaataaggc atttcttaac atttcttgtg aaacatacaa
     2521 caaaataaat ttatagaatc aaaagattgg gagtctaaaa gagttaaaaa cgatacagaa
     2581 ttgattgaaa aaataaataa attagagatt ataagtgaat atactacaat ttctgtagtg
     2641 tatattgtta aaaacgaaag agattatatt ataaaaagcc ttttgagtat tttagattta
     2701 gccgatgaaa tcattattgt tgacactggt tctgaagaca acactttaga tttaattaaa
     2761 aaaatgtgtg ataaaaaaat aaaaatattt acatttaatt ggtgtgatga tttttcaaaa
     2821 gcaagaaact ttgcaaatgc taaagcaact tgtgactgga ttatgatttt agatgctgac
     2881 gaaattgtta gaaaaaatga taatttaaag ttttacttaa cgtatttaag aattttcgac
     2941 gactttgaaa atacggcctt taattttaaa gtaattagag atgaagaagt atataaaaca
     3001 agtaagttga taagaaatac taatactttg acgtacaaag gtagagtaca cgaaacattc
     3061 ttttctttaa ataattctgt atcttatgct aatttaaatg tagaagtttt aggaattaga
     3121 agaggaagtc aaaagaaaac aaattattat aataaattgc ttcttaaaac aattatagat
     3181 ttcccaaaag aaggtcgttg gtattattta tatttgaggg ataatataga agatataaat
     3241 tataataaaa tgttaaaatt ttgtaaaaaa attatattta aaagagatgc gatatctgga
     3301 atcgaaataa acaacaatta ttactctgtt agaattattt ttcttttgat gtttaagtta
     3361 ttagaagaat ccaatcatga acgttttact tactatatgg agctgttaga gaaagatttt
     3421 aatcatactc aagactttat ttttttgaaa tattcatact atataaagaa acttaatata
     3481 gatgctacaa atttattgaa aagtttctta gagaattatg atttgttaaa tgagggagat
     3541 gtaatttttt cgaaagaatg cactcattcg ttattaggag cctctttatt ttggggagga
     3601 tatttaaagg aaagtaaaaa agtatttgaa gaattagaaa taaatagtaa agatacagtt
     3661 tgtttttata ataaaaaatt tatttatgat tgtctatcga ctttaagcta ataaatattg
     3721 atattattat agctcaggaa tctaccgcat taagataaca ggtagtgtaa aggagtgaga
     3781 aatctatgat taaaatatct ttaaagaaaa ttttatgtca attatctcaa aaaaaacttt
     3841 atgaaaaaac atttcaaagt atttatatag ttgattttag tcttttagat agggctcctc
     3901 tatttaaaga cgaatttaaa gtaataggaa cttggtatag ctatagtggt aagcgttgga
     3961 tatgccatac ggaattatca accgagcaat tcaagaaaat gataacaaaa aatatagacc
     4021 ataaagattt agaaaaagtc aaattttatt tggattatct cccattttct ataactaacg
     4081 agattccgtt ttgaaaataa atcatacatt taattgattt ttaatagtat tattaaattg
     4141 ttctatttag gagggataga tataaaaagt attgataaat ttaaagtgat atcaaatcag
     4201 gtgctgaaag acttaagtgg tgggatgtcc cgttgtaaat gggctcagat tacatgtata
     4261 aacgacagta tattggttag attcggtaca tctgatccag agagtgcatg tgattatatg
     4321 aaaaaaatat gtaaagatga ataaccatta aagcttttaa taataggatt gaattttgac
     4381 atgatattaa gtgggtggga attaccgccc gctttttgaa tatttaaatg ttgttctaaa
     4441 aagtggtggg aaggagtaga agtgtcgaat gaaattttca aaacgatttt atcgtgcaca
     4501 agtagattct caagattgtg gcgcggcagc gctcgctatg atattagaat tttacggttc
     4561 tcactattct ttggattttc tgagaagaaa gttaagaaca acagtaaatg gcactacagc
     4621 atatggtttg gttcaggtag cagataagtt aggttttgag acagttccta ttaaagcaga
     4681 tatatctttg tttgaaattg atgatttaca gtatcctttt atcgttcatg ttaaaaaaaa
     4741 taacaatcat tttcactatt atgttgtaat tagatgtgat aaaaagtata tctatatagc
     4801 tgaccctgtt gttaatttaa caaagatgcc ttatagggaa tttaaagaag agtggactgg
     4861 agttacaatc ttattaaaac ctacatcttt atataagcct ataaagcaag ataaagttcg
     4921 tttaacttat tttatacctt tattattaaa agaaaaaaag attgattata aaaataataa
     4981 tcatttccat tttagtcaat atgataaata ttttaggttc atattttatg aagtcaatta
     5041 ttgattttta tattccccaa aagttaaaaa catgggttag tatgatttcc atggctttaa
     5101 ttgcttctta cattatgcaa caaattctta gttatttgca gaaatatttt ttgattttat
     5161 tagtaaagac atattctata aatatacttt caccatacat taaacatttg tttagattac
     5221 ctattacttt ttttgaaacg ggaagagcag gagacatact atcaagactt aatgatgcaa
     5281 attcaattac tgatgcttta gctagtacca tgctatcatt gtttttagat gttacaataa
     5341 tagttggcat atctatcttc ctgtattatc aaagtactat tttatttgta ttaatattaa
     5401 ttatcattcc tatttatgcc gtaattgttt ttggttttat gaactctttt gaacggaaaa
     5461 gaataaaatc tatggaggca aatgctactt tgtcttctta tttgatggaa aatataacag
     5521 gtatggagac gataaaagca ctgacatgtg aaaagaaatg ctataagcag ataaaatata
     5581 atttttcgaa ttattgcgaa aaaacattct tgttaaacaa actaaatata aagcaaaacg
     5641 ttttgaaaaa ggttgttcaa ttaatattaa atgtatcgat tttatgggta ggcgctaacc
     5701 tagttataga taatcaaatc tcaattggaa ctttgcttgt ttttaattca ttaatgattt
     5761 actttatgaa tccaatagaa aatattatga atttacagct taaattacaa atagcaggtg
     5821 tggctaataa tcgattgaat gaaatattca cagttaaatc agaatttgaa aagaataaat
     5881 tggatatcaa attgaaccaa aactcaagta aacttatatt ttcaaatatt tcatatggat
     5941 atgtgtacga aaaagaaatc cttacagata ttaatataac aatagaacat aatagtaaag
     6001 tagcctttat tggagagtct ggatcaggta aaacaacttt ggcgaaattg atggttagtt
     6061 tttttgaacc ttcaaagggt aaaataacac taggtggatt ggacataaca aatatcaata
     6121 aatctgtttt acgacagcag attatataca ttccacaaca aacttatatt tttaatggaa
     6181 ctatacttga aaatctttta ttaggggtag ttaaagaggt tacgcaaaag gacatagata
     6241 gtgctattaa aatggttgaa ctaaaatcct ttattgatag cttaccactt ggacttgata
     6301 gtgtattaag ttctgatttt tctgctttgt caggaggtca gcggcagaag atagctttag
     6361 caagagcact attaacaaat tcttctatat tgataatgga tgaagcaacg agtaatattg
     6421 atatactcac ggagagaaaa attattaata atttgatggc gttaaataaa actattatat
     6481 ttatctccca taatctaaat ataactcaaa aagtagataa aatatttgtt ttagcaaaag
     6541 gaaaaattat agaagagggc tcacatgaat ttttattaaa agcaaaaggt tactattcta
     6601 acttaattaa ttaataatgg tattttttgt attaacacaa ccaatgtgaa gaacgaaatg
     6661 aaggtactgt tttttcacac cttttatttt ctgaatttta caattataac ttaagttaat
     6721 tattatattt tggtatattg gttgatattt tatatagtca acagtgtgaa atctttgttg
     6781 tgagttgtta aagagtattt ttaatgtatc gatatatgta cacatcaaaa aaccgctcag
     6841 tgttttatca cattgaacgg ttttttgaat ttaccctaaa gcaacatcta atatcatcat
     6901 gatggtaaag ccaaccatca agcttagtgt cgcaagatct gtgtttttac cggcttgtga
     6961 atcaggaatt aattcttcaa cgacgacaaa aatcatcgcc cctgcagcaa aagcgagtgc
     7021 gtatggtagt atcggagtaa cgaccaacac agcagctgcg cccactgtag caaaaacagg
     7081 ttctacaagt gctgaagctt gtccatagtt gaaggctttc catttggact gtcctgaagc
     7141 atgtatcggt aatgataatg cagccccttc aggaatgttt tgaatcccaa tcccaattgc
     7201 aagtccgagt gcccctaaca aagttgcgtg ttcatttcct gtcgcaatac caccaaaggc
     7261 aaccccgatt gcgagccctt caggaatgtt atgcaatgta atcgcaagta ataaaagtgt
     7321 gtttttattt agactcgtct caacaccttc ttgatgttgt gattcatcag ttgcgtttcg
     7381 atgcatgtgt ggaataacat agtctaaaga acggatgaaa accccgccga gtaaaaagcc
     7441 aattgcagca gggagccacg gtacagtact gcccgaactg ctttcaatgg ccggttgaag
     7501 taatgaccaa aaacttgcgg cgatcataat accagcagca aagccttgca tcgaattcaa
     7561 cactttttcg ttgacacttt taaatacaaa tacggttgca gcccctaatg cggttaataa
     7621 ccacgtgatg acacctgcaa gtaatgcttg taaaaagggc ggcatgtttg aaatataatc
     7681 caacatagaa atagctcctt gtatcatttg ttccaaaaaa agtgtataat atatagaact
     7741 gatttaaggt taacagataa atgcgagtaa atctataaga aaaagggtgt atgtgttgtg
     7801 gaagcgtata aaattgaacg cctcaacaaa tcttatgcgg ataaagtcat ttttgatgat
     7861 ttacagttat ccatttccaa tcaagagcga atcggattag ttggtattaa tggaacgggg
     7921 aaaagttcat tgctcaaagt catcgcaggc atggatgaag attttgatgc cgttcaatcg
     7981 catccgaaac aatatcgtat tcgttatgcg gcacaaaatc ataatttgga gccaacgtta
     8041 acggtttatg aagcggttta tagtgcagat tccaaagagt tgcaagtcat taaacgttat
     8101 gaagaagcta ctgcacaata cgcagaacaa cagactgaca aagcgtttga tgcaatgatg
     8161 gcagctcaag ctgatatgga tgcttacagt gcttgggatt atagtgctga aattaaaacg
     8221 attttatcga aacttggcat ccatgatacg acacgccgaa tgagtgaact ctcaggtggc
     8281 caacaaaagc gtgtagtgtt agcgcgtacg ttgattgaaa aaccagattt attattatta
     8341 gacgaaccga cgaaccattt agatttcgaa tcgattcatt ggttaattca atttgtaaag
     8401 tcttatccga aaacagtcgt ttttgtcacg catgaccgtt actttttaaa tgaagtggcg
     8461 acgcgtattg tagaattgcg aaatggcaag ctagatacat atccaggtaa ctatgaagcg
     8521 tatattgcaa tgcgagcaga gcgagaagaa atcgaagtgc gtcaacaaac gaaacaacgt
     8581 gcacttttta aacaagagtt agcgtggatg cgtgccggtg tcaaagcgcg aactacgaag
     8641 caacaagcaa ggcaagatcg ttttcatgac ttgaaagaac aagtacaatc gcatcaagtg
     8701 caagggaagg ggtcattgaa tctggcttat tcgcgtttag gcaaacaagt ctttgaactt
     8761 gaaaatgtga cgaaacgtat tgatggccgt acattatttg aaaacgtgtc gacattgatt
     8821 caaaaaggtg ttcaaatcgg tattgtgggc ccaaacggtg tcggcaaaac gacgatgctg
     8881 aacattttag caggtgaaga tcagcatttt gaaggtgtat taaagatagg tcaaacggta
     8941 aaagtggcat attttaaaca aacagatgaa cgattgaatc gtgatgtccg tgtcattgat
     9001 ttcttgcgtg aagaaagtga gttggcgcgt caaaaagatg ggacgaccgt ttcgattacg
     9061 caattgttgg aacgcttttt attccccagt gtgacgcatg gtaagaaagt gtataaactc
     9121 tcaggtggcg aacaaaagcg tttgtattta ttgaagttgt tagtgcatca gccgaacgtg
     9181 ttgttgttag acgaaccgac aaatgatttg gatacagaaa cgttaacgat tttagaagac
     9241 tacattcaaa catttggcgg taccgtcatt actgtgagcc atgaccgtta ctttttagat
     9301 aaagtcgtgg attgttattg gtatattcac gacggtcaaa ttgacatgat tttagggcag
     9361 tttgaagatt atttagcata ccaaaaacaa ttggaacaag caacaactca agtagcgtcc
     9421 gtgaaacaac aaccgacatt acaaccaaaa aagaaaaaag gattatcata caacgaaaaa
     9481 cgagaatatg acatgttatt agtgcgcatc gaggaaacag agtcgcgtct ggctgaaatt
     9541 gacgccgaaa tgatcgaagc gaacgcagat tatgccaaaa tcaaagcatt aaatgaagaa
     9601 aaagaaacgc tcgaagctca atatgatacc gacatgacgc ggtggagtga actagaagaa
     9661 atattagaac aatagaggga tagcatggag caaacattgt cgcattattt tggatacaaa
     9721 tcttttcgtc ctggtcaaaa agaaattatt gaacgcgtat tgaatcatca tcatacgtta
     9781 ggggtgttac ccacaggtgg gggtaaatcg ttatgttatc aagtaccggg attgatgttt
     9841 aaaggaacga ccattgtcat tagtccttta atttcgttaa tgaaagacca agtggaccaa
     9901 ttgaccgcaa tgggaatatc tgcggcttat ttgaatagta gtttaagtac gcaagcacaa
     9961 aaagagatag aaacgcaatt ggtcaatggt gagttgaaat ttttatacat cgcaccggaa
    10021 cgttttgatc aaacttattt tgaaatgttg ttgcgacgtg tcgatatttc actcgtcgca
    10081 tttgacgaag cacactgtat ttcaaaatgg ggacatgatt ttcggccgag ttatcaagca
    10141 gtcatacaac gcgtattgac tatgccgcaa cagttttcga ttgtcgcatt gactgcaaca
    10201 gcgacgactg aagtgcaaca agatattatg gaacggttaa tgatttcgcg acaacactta
    10261 gtcaaaacta gtattcgtcg acgtaatttg aagttccagg tgaatgggac gtatcaacgt
    10321 caaaagtttg tattggatta tattcaaaaa catagtcaga aagcggggat tgtttattgt
    10381 tcaacgcgta aacaagtaga agccttgtat gaagctttag aagacgcaga tatccctgta
    10441 tcctattatc atgcaggaat gacagcgaaa caaagagatg aagcccagaa tgacttttta
    10501 tatgaccgta ctcgtgtagt cgttgcgacc aacgcattcg gtatgggaat tgataaatcg
    10561 aatgtccgtt atgtcattca ttacaatatg ccgggtgatt tggaatcgta ttatcaagaa
    10621 gcaggacgtg caggtcgaga cggattagat agtgattgta ttttattgta cagtgatcga
    10681 gatattgctt tacatcagta ttttatcggt gcctcgaaag cggacgatga ttacaaggaa
    10741 cgtatgggcg aaaaattgac gaaaatgatt caatatacga aaacgacgaa atgtctagaa
    10801 gcaacattag tccattattt tgagccgaat gaaaaacttg aagaatgtca ccaatgtagt
    10861 aattgtattg ataaaaataa aacgtacaat atgacgaaag aagcgaaaat gatcattagc
    10921 ggtgtcgcac gtttacgtca acaagaagga tatcaaacgg tcattcaagt tttgcgtggc
    10981 gaggtttcgg actacattcg tcatcaaggc tatgaaaatc tttcgacgta tggtttgatg
    11041 aaagactata cgactggcga gttacatcat ttaatcgatg aactccgctt taaaggcttt
    11101 ctcaacgaac atgacgaaat actcatgtgt gatgcctcag tagaaaagtt attgagtgag
    11161 gatacacaag tgtttacaag gccgttcaag cgtaagtcga aagaaacagt gcatattaac
    11221 actgtagaag gggtcgaccg cacgttattt gaaaagcttg tcgaagtcag acgtgaaatg
    11281 agcgaacgat taaacatgcc accgacgaat atttttacag actatacgtt agaagctttt
    11341 gcgaaaagaa agcctgtcac aaagcaagaa atgattcaga ttgatggtgt tggtagttat
    11401 aagctgaagc actattgtcc tgaattttta gaagcgattc aagaatataa aatgcatcgt
    11461 tcgtaaaaca tcagatggag ctgggggaat tcctggctcc ttttttacaa ataaacgatt
    11521 tcttattgag tggtaaagaa tggaaaagtg agacgtgtcg atagtagatg atttaaattt
    11581 tttagatgca aattgtgtta tttttacaat gaat
//
