LOCUS       NZ_CP015098            10951 bp    DNA     linear   CON 03-MAR-2024
DEFINITION  Streptomyces qaidamensis strain S10 chromosome, complete genome.
ACCESSION   NZ_CP015098
VERSION     NZ_CP015098.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces qaidamensis
  ORGANISM  Streptomyces qaidamensis
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces; Streptomyces aurantiacus group.
REFERENCE   1  (bases 1 to 9083372)
  AUTHORS   Zhang,B.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2016) State Key Laboratory of Cryospheric
            Sciences, Chinese Academy of Sciences, Donggang West Road 320,
            Lanzhou, Gansu 730000, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SMRT Analysis v. 2.3.0
            Genome Coverage                   :: 278x
            Sequencing Technology :: Illumina HiSeq 4000; PacBio RSII
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 03/03/2024 10:55:15
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,226
            CDSs (total)                      :: 8,137
            Genes (coding)                    :: 7,871
            CDSs (with protein)               :: 7,871
            Genes (RNA)                       :: 89
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 119 of 266
            Pseudo Genes (incomplete)         :: 213 of 266
            Pseudo Genes (internal stop)      :: 25 of 266
            Pseudo Genes (multiple problems)  :: 80 of 266
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 16:26:55
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 4778267
            Orig. end                         :: 4789218
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP015098.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10951
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="butyrolactone"
                     /region_number="13"
                     /rules="AfsA"
                     /tool="antismash"
     cand_cluster    1..10951
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="AfsA"
                     /kind="single"
                     /product="butyrolactone"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10951
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5951](+)"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5951
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
     gene            complement(207..920)
                     /locus_tag="A4E84_RS21235"
                     /old_locus_tag="A4E84_21235"
     CDS             complement(207..920)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1016: LuxR family
                     DNA-binding response regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013002045.1"
                     /locus_tag="A4E84_RS21235"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A4E84_21235"
                     /product="response regulator transcription factor"
                     /protein_id="WP_062928105.1"
                     /transl_table=11
                     /translation="MTIRVVVADDQELVRSGFAMILDAQPDIEVVAEAGDGGGAVDAVR
                     RHAPDVALLDIRMPRMDGIEACRAISAASDCRTVMLTTFDSDEYVYDALHAGASGFLLK
                     DVRRDDLVHAVRVVARGDSLLAPSVARRLVEQYTRPATARARRPDPRLDVLTARERETL
                     LLLARGLSNAEIAAELVVSDHTVKTHVGNVLAKLGLRDRIQAVICAYETGLVAAGDPPA
                     GGAAGAGSSPAPVRK"
     gene            complement(917..2086)
                     /locus_tag="A4E84_RS21240"
                     /old_locus_tag="A4E84_21240"
     CDS             complement(917..2086)
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA]"
                     /GO_function="GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /GO_process="GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1048: sensor
                     histidine kinase"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015036506.1"
                     /locus_tag="A4E84_RS21240"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A4E84_21240"
                     /product="histidine kinase"
                     /protein_id="WP_062928106.1"
                     /transl_table=11
                     /translation="MGMRGELRGWRERGRVLAAAHPLLVDLGVALLVQGAMTMPFLVPR
                     APGLPAATWAAYGLSTLTVVPLVWRRRAPVAVLFAVLATSLLYRLAVDGPGQPLPYTGL
                     VVVYTIAALSPARKRAVTGLVLLIAVPVGVWLNTRSARELTFSVFVFAAAYVFGRLTDA
                     RQRAHRIEAEQAAARERARIAREMHDILSHAVSLMIVQAEAGPVAVRSAPERAEAAFDA
                     ISATGRDAMAQLRHMLGVLRDGGDDSGGSPRQPQPGIAALPGLLDRVRGSGLDVVYRTA
                     GDAGPVADGVGVTVYRVVQEALTNVVRHAEAQTATVELIRRGNELEVTVTDDGRGAQTV
                     SRRGGHGLAGIRERAAAHGGDAVTGPGADGHGFEVRVRIPLSAAAEVKT"
     gene            complement(2169..2552)
                     /locus_tag="A4E84_RS21245"
                     /old_locus_tag="A4E84_21245"
     CDS             complement(2169..2552)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010032318.1"
                     /locus_tag="A4E84_RS21245"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A4E84_21245"
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="WP_062928107.1"
                     /transl_table=11
                     /translation="MSATVRQAPVIKVVPEPEDLPEPLPEPAVGELRLETVLGALSDPL
                     RLRIVRKLLLESEAFDHSCGWFGLDRPKSSLTHHFKALREAGVTRQRQYGLERRSHVRV
                     DDLNARFPGLVDLVAAWTPPNAP"
     gene            2650..3933
                     /locus_tag="A4E84_RS21250"
                     /old_locus_tag="A4E84_21250"
     CDS             2650..3933
                     /GO_function="GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1005: Drug
                     resistance transporter, EmrB/QacA"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007387370.1"
                     /locus_tag="A4E84_RS21250"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A4E84_21250"
                     /product="MFS transporter"
                     /protein_id="WP_062928108.1"
                     /transl_table=11
                     /translation="MSESRTLSAETVDEQQAAVPEPATPAPPERAAAARTPTWWVWLAA
                     WPVTAVFVLSNAATPLYVLWQRDIGFSKGTLTVVFAFYIVGLLGSLLVSGVVSDRLGRK
                     PVLLPALALALAACAIFATATTVVALIVARLFTGIAVGAVVSAGMAAVTDVAGPERKRL
                     AALLASCAMVFGAGLGPLLAGLLSETLPAPTVTVFVVEAVLLLTAVLAVLRMPVRRPAA
                     RGKGPWVRVPGVPHGNGIHLALGIAVFAPGITATSFVLSLGPTLLTELLGTTSRIVAGA
                     MAFVMFLAATGVQFAVQKLPRRTILTAGAISTTLSMAALIAAVHASSVALLVASALLAG
                     AGQGMGQLGGLSLLNSTVPAQRLAEANAALNVGGYIPAGLLPVSTGYLMDAVGLPTGAT
                     LFGTVLMGLAVVGGLVVLSGHRRVSEPA"
     gene            complement(4057..4710)
                     /locus_tag="A4E84_RS21255"
                     /old_locus_tag="A4E84_21255"
     CDS             complement(4057..4710)
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1057: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011039544.1"
                     /locus_tag="A4E84_RS21255"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A4E84_21255"
                     /product="ScbR family autoregulator-binding transcription
                     factor"
                     /protein_id="WP_062928109.1"
                     /transl_table=11
                     /translation="MSEPQPAIAAFAPRKFVTRRGPDPKQERSARTRLLVLTAASELFA
                     EQGFRHTSVKDVADRMKMTKGAVYFHYPSKEALAVAVVEEHYARWPALLEEVLTEGYGP
                     LETAEQMLARAAVSFRDDIVVQAGARLQIERPHIDAELPTPYVDWTELLASLLVAARDE
                     GQVREGVSPEAAARSLVSAFFGAQHISDVLRGRADVVERWTETSELLFHAIRAH"
     misc_feature    4790..4808
                     /note="TFBS match to LexA_variant_3, Repressor of DNA
                     damage response, confidence: weak, score: 18.64"
                     /tool="antismash"
     misc_feature    complement(4829..4845)
                     /note="TFBS match to ZuR_variant_2, Zinc-responsive
                     repressor, confidence: weak, score: 15.44"
                     /tool="antismash"
     gene            5001..5951
                     /locus_tag="A4E84_RS21260"
                     /old_locus_tag="A4E84_21260"
     CDS             5001..5951
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     butyrolactone: AfsA"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF041195.1"
                     /locus_tag="A4E84_RS21260"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A4E84_21260"
                     /product="ScbA/BarX family gamma-butyrolactone biosynthesis
                     protein"
                     /protein_id="WP_159029602.1"
                     /sec_met_domain="AfsA (E-value: 6.3e-20, bitscore: 69.9,
                     seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MARESVHKSASTEVLLTDARHLGEDRFAVAARLPREQFITADHPG
                     EGALDPVLLAEAARQAAIHLSHRFHDIPLGHPFVLGEVAVELDEPLPPSADATLQAHCR
                     RKAGNPRRACLELDATVWAAGRAAGRARVLWEAMEPRRYAVLRKRGAAQPPVVSGPAHA
                     AAAGAASLPPARVGLRRDRDVLLASDTERSGRWWLRLDPGHPVLFDHPSDHIPGMALVE
                     AFRQAAGVAATQGAVGPARVRDIGELTVAYDSFGEPDLPVAITLEPRGAGAREPHDEHA
                     RLVHLTATQGDRTLARARIRCGTPGRAHRRTGAAC"
     misc_feature    5097..5099
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            5945..7075
                     /locus_tag="A4E84_RS21265"
                     /old_locus_tag="A4E84_21265"
     CDS             5945..7075
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1191: acyl-CoA dehydrogenase type 2"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011039546.1"
                     /locus_tag="A4E84_RS21265"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A4E84_21265"
                     /product="oxidoreductase"
                     /protein_id="WP_062928111.1"
                     /transl_table=11
                     /translation="MLIDDPERAALRTPLRDLSALTATARRDAPVAEERRALTADAVDA
                     LTRAGFPRHFVPGRFGGEAGEFVPLVAGVADAGEACAATAWCAALFAAHGRLAAYLPPE
                     GQREVWADGPDVRIAAAVVPPQGAAVAVDGGWRLDGAWRPASGVDHADWILLASWTDGE
                     EGREHRIFAVPRAQVTVEDTWHSLGLRGTGSNTVRCEGLVVPRHRTYTLDALLRSRPGA
                     ARCHTVPYPMVAALMFAAPLLGAARGALRAWTGERLAAERRHPAAHTVLARASARIHAA
                     GLLLTGAAERADRGEITPLAVAENRRDATAAATLCREAVDALFHASGLRAQSPDSAVQR
                     AWRDATAIAAHAALDPDAAARAYAEAVLSPAGGDGP"
     gene            7072..7875
                     /locus_tag="A4E84_RS21270"
                     /old_locus_tag="A4E84_21270"
     CDS             7072..7875
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /GO_process="GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019981291.1"
                     /locus_tag="A4E84_RS21270"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A4E84_21270"
                     /product="HAD-IB family hydrolase"
                     /protein_id="WP_062928112.1"
                     /transl_table=11
                     /translation="MTRAAAVPVAFFDVDETLVATKSLLDFWDYWVTRQDGPAGTGRGP
                     YDPQRRTAQPAPPATTSTAAAGPPDRSRLNRDYYRRYTGTPESLLQAAARDWYARYRRG
                     ERAFVTAGLHALARHRALGHEIVLVSGSLRPVVDAVAADLGATAVRCAEQTVTDDGLLT
                     GEIDRPMIGRAKADAVEAHLAERGARAGDCHAYGDHDSDLPMLRSVGHPVVIGGAPALR
                     HEAARLGWTTLPGDTGPHPARPPVATGRPPRSAAGQRQDQASVSP"
     misc_feature    complement(7907..7922)
                     /note="TFBS match to CsoR, Copper-responsive repressor,
                     confidence: weak, score: 22.0"
                     /tool="antismash"
     gene            7959..8570
                     /locus_tag="A4E84_RS21275"
                     /old_locus_tag="A4E84_21275"
     CDS             7959..8570
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1057: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /locus_tag="A4E84_RS21275"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A4E84_21275"
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="WP_062928113.1"
                     /transl_table=11
                     /translation="MAKQERALRTREKVLDAAAEEFAAQGYAKATLNDVARRTGMTKGA
                     LYGHFPSKEILAATLLAQSRVAWEEMRRARDVPGAAARAVLEELVLELARRLRSDIRLR
                     AALRLASDLPCLARAAPDLFDDVRRHLVGLVHRAQQEDGIAPYPPDLVVELLLTSLHGT
                     LHASQCDDRYGSAPTREAVWRLLLDALAGDRGGCRGMAGR"
     misc_feature    complement(8589..8598)
                     /note="TFBS match to ToxT, Regulator of MSHA biosynthesis,
                     confidence: weak, score: 13.37"
                     /tool="antismash"
     misc_feature    complement(8605..8618)
                     /note="TFBS match to LexA_variant_2, Repressor of DNA
                     damage response, confidence: weak, score: 18.02"
                     /tool="antismash"
     gene            8847..9743
                     /locus_tag="A4E84_RS21280"
                     /old_locus_tag="A4E84_21280"
     CDS             8847..9743
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1014: LysR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /locus_tag="A4E84_RS21280"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A4E84_21280"
                     /product="LysR family transcriptional regulator"
                     /protein_id="WP_063827569.1"
                     /transl_table=11
                     /translation="MRHLRYFLAVVRSGTVSAAAKELRISQPSLSQQIKRLEQRIGAAL
                     FVRSSRGVELTAGGRAFLREIQSIPGQLRSAIAAAAPTPALWSVGVCGGVPAEVLAEVQ
                     NGLAGQGAHHVGSRPGPHLRMRSVGAAEQPELLEHGEIVFGIMRLPVTAPHLARAVVWD
                     EPLGVVLPTAHPLASQPSLTWEDLSTQRLLWYDEGCAPGYADAVPAQLTALGWKAVLHP
                     VDQDQQALFVHALQTTRDLVALRPSTAVTDDPQLVWRPLPTASAPRERLAVTALAGSRA
                     ARYLGRVAARCGWPYRA"
     misc_feature    9826..9837
                     /note="TFBS match to CcpA, Catabolite control protein A,
                     confidence: weak, score: 15.92"
                     /tool="antismash"
ORIGIN
        1 gcggccgtcg acgcggcggg ggcggaggcg gcggccggga gcgcggacgg ccccacggcg
       61 agtcccgcga cgagcacggc ggcggtcagc aggccggtac gcatcttcat ggcgaacttc
      121 cttgtgagga ggcggtgttg tgcggccgct tcatcctccg ccgccacgcc gtcccgcgga
      181 tcgcccccgc cggtggtccc gcggcgtcac ttcctcaccg gcgcggggga ggacccggcc
      241 ccggccgctc ccccggccgg gggatcaccg gccgcgacga ggccggtctc gtaggcgcag
      301 atcacggcct ggatgcggtc ccggaggccc agcttcgcga ggacgttgcc gacgtgcgtc
      361 ttgacggtgt ggtcgctgac caccagctcg gcggcgatct cggcgttcga cagaccgcgc
      421 gcgagcagca gcagcgtctc gcgctcccgg gcggtcagga cgtcgagccg gggatcgggc
      481 cggcgggccc gggcggtggc gggccgggtg tactgctcga ccagccgccg ggcgacggag
      541 ggggcgagca gtgagtcgcc ccgggcgacg acccgcacgg cgtgcacgag gtcgtcgcgg
      601 cggacgtcct tgagcaggaa gccgctcgca cccgcgtgca gcgcgtcgta cacgtactcg
      661 tcggaatcga aggtcgtcag catcaccgtc cggcagtcgc tcgccgcgct gatcgcccgg
      721 caggcctcga tgccgtccat gcggggcatc cggatgtcga gcagcgcgac gtcgggcgcg
      781 tgccgccgta ccgcgtcgac ggccccgccg ccgtcacccg cctcggcgac gacctcgatg
      841 tcgggctgcg cgtccaggat catggcgaag ccgctgcgca cgagttcctg gtcgtcggcg
      901 acgacgacac ggatcgtcac gtcttcacct ccgccgcggc ggagagggga atccgcaccc
      961 gtacctcgaa gccgtgcccg tccgctcccg gcccggtgac cgcgtcgccc ccgtgggcgg
     1021 cagcccgctc gcggatgccg gcgagcccgt gcccgccccg gcgggacacg gtctgcgccc
     1081 ctcgcccgtc atcggtcacc gtgacctcca actcgttccc cctacggatc agttccactg
     1141 tcgcggtctg cgcctccgcg tgcctgacga cattggtcag ggcctcctgg accacccggt
     1201 agacggtcac cccgactccg tcggcgaccg gcccggcgtc ccccgcggtc cggtacacga
     1261 cgtcgagtcc gctcccgcgc acccggtcga gaaggccggg cagcgcggcg atcccgggct
     1321 gcggctgtcg cggggagcca ccgctgtcgt cgccgccgtc ccgcagcacc cccagcatgt
     1381 gacgcagttg cgccatggcg tcccgcccgg tcgcggagat ggcgtcgaag gcggcctcgg
     1441 cccgctcggg cgcggaccgt accgcgaccg gtccggcctc ggcctgcacg atcatcaggc
     1501 tcacggcgtg ggagaggatg tcgtgcatct cccgcgcgat ccgggcccgt tcgcgagcgg
     1561 cggcctgttc ggcctcgatg cggtgagccc gctgccgggc gtcggtcagc cgcccgaaga
     1621 cgtaggcggc ggcgaacacg aagacggaga aagtcagttc acgcgccgac cgggtgttga
     1681 gccagactcc caccggcacg gcgatcagca gcacgagccc ggtgacggcc cgtttccgcg
     1741 cgggcgacag cgcggcaatc gtgtacacga ccaccagtcc ggtgtacggc aggggctgcc
     1801 cgggcccgtc gacggccagc cggtacagca ggctggtggc cagcaccgcg aacagcacgg
     1861 cgaccggtgc ccgcctgcgc cataccaggg gcacgacggt gagggtgctc agcccgtacg
     1921 cggcccaggt cgccgcgggc agcccgggcg cgcgcggcac caggaacggc atggtcatcg
     1981 caccctggac gagcagcgcg acgccgaggt cgaccagcag cggatgggcc gccgcgagca
     2041 cccggccccg ctcccgccac ccgcgcagtt ccccgcgcat gcccatggcg cccccccgtc
     2101 ccgttgcggc ccgaccccgg aaccgtagcg gggtggtggc cgcacgcgcc gggagaacaa
     2161 gccctggttc agggcgcgtt gggcggtgtc caggccgcca ccaggtccac cagcccgggg
     2221 aaccgggcgt tgaggtcgtc cacgcggacg tggctgcggc gttccaggcc gtactggcgc
     2281 tggcgggtga cgccggcctc gcgcagggcc ttgaagtgat gggtgaggga ggacttgggg
     2341 cggtcgaggc cgaaccagcc gcaggagtgg tcgaacgcct ccgactcaag caggagtttg
     2401 cgcacgatcc gcagccgcag cgggtcgctc agcgcgccca gcacggtctc cagccgcagc
     2461 tccccgaccg ccggctcggg cagcggttcc ggcaggtcct ccggctccgg caccaccttg
     2521 atgaccggcg cctgtcgaac cgtcgcagac ataggcaact ccctataacc gaaatcctat
     2581 gtacgagttc aatcgtactg catgctaagt tcgaatggac tcgtacttag ctgccggagg
     2641 gagcaaccca tgtcggagag tcggaccctg tccgccgaga cggtggacga acagcaggcc
     2701 gcagtgccgg agccggccac gcccgcaccg ccggagcggg ccgcggcggc ccgcacgccc
     2761 acctggtggg tgtggctggc ggcctggccg gtcaccgccg tcttcgtgct gtccaacgcg
     2821 gcgaccccgc tgtacgtgct gtggcagcgc gacatcgggt tctccaaggg cacgctgacc
     2881 gtcgtcttcg cgttctacat cgtcggactg ctcggctcgc tgctcgtgtc gggcgtcgtc
     2941 tccgaccggc tgggacgcaa gcccgtgctg ctgcccgccc tcgccctcgc cctggccgca
     3001 tgcgcgatct tcgcgaccgc cacgacggtc gtcgcactga tcgtcgcccg gttgttcacc
     3061 ggcatcgccg tcggcgcggt cgtctccgcc ggtatggccg cggtgacgga cgtggcaggc
     3121 ccggagcgga agcggctggc cgcgctgctg gcctcctgcg cgatggtctt cggggccggc
     3181 ctcggcccgc tgctcgccgg cctgctgtcc gagacgctcc cggctcccac cgtcaccgtc
     3241 ttcgtcgtcg aggcggtcct gctgctcacg gccgtcctcg ccgtgctgcg catgccggta
     3301 cgccgtcccg ccgcccgcgg caagggcccc tgggtgcgcg tgcccggcgt gccgcacggc
     3361 aacggcatcc atctcgctct gggcatcgcg gtgttcgccc ccggcatcac cgccacgtcc
     3421 ttcgtgctgt cgctcggccc caccctgctg accgaactgc tcggcaccac cagccggatc
     3481 gtcgccggag ccatggcctt cgtcatgttc ctcgccgcca ccggcgtcca gttcgccgtg
     3541 cagaagctgc cccggcgcac catcctgacc gccggtgcga tcagcaccac cctcagcatg
     3601 gccgcgctga tcgccgccgt gcacgcctcg tcggtcgcgc tgctcgtcgc ctccgccctg
     3661 ctcgccggag ccggccaggg catgggccag ctcggcggtc tgtcgctgct caactccacc
     3721 gtcccggcgc agcgcctcgc cgaggccaac gccgcactga acgtgggtgg ttacatcccc
     3781 gccggcctcc tgccggtgtc caccggctac ctcatggacg cggtgggcct gcccaccggc
     3841 gcgaccctct tcggcaccgt cctgatgggc ctcgccgtcg tcggcggact cgtcgtgctc
     3901 tccggccacc gccgggtgtc cgagccggcg tgacgggacg gcgccggtgc cgggtccccg
     3961 ggcgtacccg cgtggcggtg ctccacggct tcggccgcgg agcaccgcca tgcacgtgtg
     4021 cgccccggcg cccggagccg cttccgaggc cccgggtcag tgggcccgga tggcgtggaa
     4081 gagcagttcg ctcgtctccg tccagcgctc caccacgtcc gcgcgtcccc tgagcacgtc
     4141 cgagatgtgc tgggcgccga agaacgccga gaccagggac ctcgccgccg cctccggcga
     4201 gacgccctcc cgcacctgac cctcgtcccg cgccgcgacc agcagcgagg cgaggagctc
     4261 ggtccagtcg acgtacggcg tcggcagctc ggcgtcgatg tgcgggcgct ctatctgcag
     4321 gcgcgccccc gcctgcacga cgatgtcgtc ccggaacgac accgccgcac gcgccagcat
     4381 ctgctccgcc gtctccagcg gcccgtaccc ctccgtcagg acctcttcca ggagcgccgg
     4441 ccaccgggcg tagtgctcct ccaccaccgc cacggccagc gcctccttgg aggggtagtg
     4501 gaagtagacc gcgcccttcg tcatcttcat gcggtcggcc acgtccttga ccgacgtgtg
     4561 ccggaatccc tgctcggcga acagctcgga cgccgcggtg agcaccagca acctggtgcg
     4621 cgcggaccgt tcctgtttgg ggtcgggccc cctcctggtg acgaacttcc tcggcgcgaa
     4681 cgcggcgatg gctggctgcg gctcggacat gctcggttcc tctgactcgg cggcctcggc
     4741 cggagctggc ggacgtgttc ggccggggca gatggaatcg ttccggccgt actggctcaa
     4801 aaacgattct cggctcgata ccttctggaa ggtatctttt ttcgcgtcgc ttcccgtgaa
     4861 cgacgggacc atagcgggct cacccctgct tccgcgaccc gaaagggaac agaaccttgc
     4921 actgcgtacc gtcccccgcc gcgccgccgg acctccacac cccgggacct cccggcggcg
     4981 cgctccgcta cgaccgcccc gtggcccgcg aatccgtgca caagtccgcc tccacggaag
     5041 tgctcctgac cgacgcccgg cacctgggcg aggaccggtt cgcggtcgcc gcccggttac
     5101 cccgggagca gttcatcacc gccgaccacc ccggcgaggg ggccctggac cccgtactgc
     5161 tggccgaggc ggctcggcag gcggcgatcc acctgtccca ccgtttccac gacatccccc
     5221 tcgggcaccc cttcgtcctc ggcgaggtcg ccgtcgaact cgacgagccg ctgccgccct
     5281 cggccgacgc gacgcttcag gcgcactgtc ggcgcaaggc cgggaacccc cgccgcgcct
     5341 gcctggaact ggacgcgacg gtgtgggcgg cgggccgggc agccggccgg gcgcgagtcc
     5401 tctgggaggc catggagcca cgccgatacg cggtcctccg caagcgcggc gccgcccagc
     5461 cacccgtggt gtccgggccg gcccacgccg ctgccgccgg ggccgcctcg ctgccaccgg
     5521 ccagggtcgg gctgcggcgc gaccgcgacg tcctcctcgc ctccgacacg gagcgctccg
     5581 gccggtggtg gctgcggctg gaccccggcc accccgtcct cttcgaccac ccgtcggacc
     5641 acataccggg catggctctc gtggaggcct tccgccaagc ggccggcgtc gcggccaccc
     5701 agggcgccgt cggcccggcc cgggtgcggg acatcggtga actcaccgtg gcatacgact
     5761 cgttcggcga accggacctg ccggtggcca tcaccctgga gccccgcggg gcaggggccc
     5821 gggagccgca cgacgagcac gcccgcctcg tccacctgac cgccacgcag ggcgaccgca
     5881 cgctcgcccg ggcccgcatc cggtgcggga cgcccggccg tgcccaccgg cgtacggggg
     5941 ccgcatgctg atcgacgacc cggagcgcgc cgccctgcgc acacccttgc gtgacctgtc
     6001 ggccctgacg gcgaccgccc gccgggacgc cccggtggcg gaggagcggc gtgcactcac
     6061 ggccgatgcc gtcgacgcgt tgacccgcgc gggttttccg cgtcacttcg tgcccggccg
     6121 gttcggcggg gaggcggggg agttcgtccc gctggtggcc ggcgtggcgg acgcggggga
     6181 ggcctgtgcc gccacggcgt ggtgcgccgc gctgttcgcc gcgcacggcc ggctggccgc
     6241 ctatctcccg cccgaggggc agcgcgaggt gtgggcggac ggcccggacg tgcggatcgc
     6301 cgccgcggtg gtaccgcccc agggcgcggc ggtggcggtc gacggcggct ggcgcctcga
     6361 cggcgcctgg cggcctgcca gcggggtcga tcacgcggac tggatcctgc tggcctcctg
     6421 gacggacggc gaggaggggc gggagcaccg gatcttcgcc gtcccccgtg cgcaggtgac
     6481 cgtggaggac acctggcact ccctgggcct gcggggcacc ggcagcaaca cggtgcggtg
     6541 cgagggactc gtcgttccgc ggcaccggac gtacacgctc gacgcactcc tgcgaagccg
     6601 tccgggtgcg gcccggtgcc acaccgtgcc gtatcccatg gtcgcggcgc tgatgttcgc
     6661 cgctccgctg ctcggagccg cccgcggggc cctgcgcgcc tggaccggcg aacggctcgc
     6721 cgccgagcgc cgccacccgg cggcccacac cgtgctggcc cgggcatcgg cgcggatcca
     6781 cgccgccggt ctgctcctga ccggtgcggc cgagcgcgcc gaccggggcg agatcacccc
     6841 gctcgccgtc gcggagaacc gcagggacgc caccgccgcg gccacactgt gccgggaggc
     6901 ggtcgacgcg ctgttccacg cctccggcct gcgcgcccag tccccggaca gcgccgtgca
     6961 acgcgcctgg cgggacgcca cggccatcgc cgcccacgcc gccctggacc cggacgccgc
     7021 ggcccgggcc tacgcggaag cggtgctgtc cccggcggga ggtgacgggc catgacccgg
     7081 gcggcagccg tcccggtcgc cttcttcgac gtcgacgaga ccctcgtcgc cacgaagagc
     7141 ctgctcgact tctgggacta ctgggtgaca cggcaggacg gcccggcggg cacgggccgg
     7201 gggccgtacg acccgcagcg acgaacggcg cagccggcgc cgcccgcgac cacgtccacg
     7261 gcggcagccg gcccgcccga ccgctcccgg ctcaaccgcg actactaccg ccgctacacg
     7321 ggcacgcccg agtcgctcct ccaggccgcc gcccgcgact ggtacgcccg ctaccgccgc
     7381 ggcgagcgcg ccttcgtcac cgccggactg cacgccctcg cccggcaccg cgctctcggg
     7441 cacgagatcg tcctggtctc cggctccctg cgccccgtcg tggacgccgt cgccgcggac
     7501 ctcggcgcca ccgccgtacg gtgcgccgag cagaccgtca ccgatgacgg actgctgacc
     7561 ggcgagatcg accggccgat gatcgggcgg gccaaggccg acgccgtcga ggcccacctc
     7621 gcggagcgcg gcgcccgcgc cggtgactgc cacgcgtacg gcgaccacga cagcgacctg
     7681 cccatgctcc gctccgtcgg ccaccccgtc gtgatcggcg gcgcccccgc cctgcggcac
     7741 gaggccgccc ggctgggctg gacgaccctc cccggcgaca ccggaccaca ccccgcaagg
     7801 ccgcccgtgg ctaccggtcg accgccccgt tccgccgcag gccaacggca ggatcaggcc
     7861 tccgtgagtc cctgatgtac ggccgacttt cgcctcctcg gaggaatcct tcccaggggt
     7921 atgtttgtgc tctcgatcaa gaccggaggg caccccgcat ggccaaacag gaaagggcgc
     7981 tgcgcacacg tgagaaggtg ctggacgccg cggccgagga gttcgccgcg cagggatacg
     8041 ccaaggccac gctgaacgac gtggcgaggc ggacggggat gaccaaaggg gccctctacg
     8101 ggcacttccc gtccaaggag atcctcgccg cgaccctgct cgcccagtct cgcgtggcct
     8161 gggaggagat gcggcgtgcc cgggacgtgc cgggcgccgc ggcccgtgcc gtgctggagg
     8221 aactggtcct ggagctcgcc cggcgcctgc gctcggacat ccggctcagg gcggccctcc
     8281 ggctcgccag cgacctgccc tgtctggccc gcgcggcccc ggacctcttc gacgacgtgc
     8341 ggcgtcatct ggtcggcctc gtccacaggg cgcagcagga ggacggcatc gccccctatc
     8401 cgccggacct ggtggtggag ctgctgctga cctccctgca cggcactctc cacgcctcgc
     8461 agtgcgacga ccggtacggc agcgccccca cccgggaggc ggtgtggcgt ctcctgctcg
     8521 acgcgctcgc cggtgatcgc gggggctgcc ggggcatggc cgggcggtga ggtcgtcgtt
     8581 tcggacacgt aaaaaatacc atcccgaagg ttttttctgt catgatgatg ctgtcccggc
     8641 ccgccgggcc gcccgctgcc cggtcgcagg ccggtccgcg gtcgcggtcg ccatgaagtg
     8701 acggagggaa gcagatgagt gtgctggatg aaaggatcgg tccgttcccc ctcgggcagg
     8761 cagccttcgg cgcggccccc gaggagccgc accaggagga gattcagcgt gctgtcagcc
     8821 atgccgaaca tgtcgatgac ttcgatatgc gacaccttcg gtacttcctg gctgtggtcc
     8881 gctccggaac tgtctccgca gccgccaagg aattgcgtat atcgcaaccg agtctgagtc
     8941 agcagatcaa acggctggag cagcgaatag gagccgcgct tttcgttcgc agctcccgcg
     9001 gtgtcgaact gacggcgggt ggccgggcct ttctccggga gatccagagc atccccggcc
     9061 agctccggtc ggccatcgcg gccgccgccc ccacccccgc gctctggtcc gtcggcgtgt
     9121 gcggaggcgt gccggcggag gtgctcgcgg aggtgcagaa cggtctcgcc gggcagggcg
     9181 cacaccacgt cggcagcaga cccggaccgc acctgcgcat gcgttcggtg ggcgcggcgg
     9241 agcagcccga gctgctggag cacggcgaga tcgtgttcgg gatcatgcgc ctgccggtga
     9301 ccgcgcctca cctcgcccgc gccgtcgtat gggacgagcc gctcggcgtc gtcctgccca
     9361 ccgcgcaccc cctggcctcg caaccgagcc tgacctggga agacctttcc acgcagcgcc
     9421 tcctctggta cgacgagggg tgcgcgcccg ggtacgccga cgccgtgccg gcgcagctga
     9481 cggcgctggg ctggaaggcc gtgctgcatc ctgtcgacca ggaccagcag gctctgttcg
     9541 tgcacgccct gcagaccacc cgcgatctgg tggccctgcg cccgagcacg gcggtgacgg
     9601 acgatccgca gctcgtctgg cgcccgttgc ccaccgcctc cgcgccccgt gaacggctgg
     9661 ccgtgaccgc cctggccggc agccgcgcgg cccggtatct gggccgggtg gccgctcggt
     9721 gcgggtggcc ctaccgcgcc tgatctccct ttttcggccc cactcgacgg ccggtccgga
     9781 acttcttgtt ccgggccggc cgttctctgt ttccccgcag gaaatgaaaa cggaaactta
     9841 ctccgaggtc accttaccgg agggtcatat tccgtcatag tcggagcctt atggctacta
     9901 cgcggtatag gtcttggaca ggcgagatcc gctgaaccta gtctcctgta tgtgacacga
     9961 atccggttcg gtcacacagc aatttccgat cgatttccca atgacgtcgg attcattcct
    10021 gatccattcg gccgcggccg ggaaaacagg ggagaagcgt gatctccacc aagctgacgg
    10081 aactgctcgg catcgaacac cccgtcatca gcgcccccat ggggtccgtg tccggaggac
    10141 ggctcaccac cgccgtcacc cgcgccggcg ggctgggact cgtcggtgtc agctacggcg
    10201 accccgcctt catcgacgaa cacgtggggc aggccgcgca ggccggtggc gtctggggcg
    10261 tcggctgcgt gatgttcacc ttcgacgagc gcccgggcct gtgggacaag gtgctcggct
    10321 acgccccgcc ggtcgtcgcc ctgtcgttcg gggacccgga ccaggtacgc cggtacgtcg
    10381 cctccgccgc cgagagcggt gcccacgtcg tggtccaggt gcacgatccc gagcaggcgg
    10441 ccgtggccct gcaggccggg gcctccgtgc tggtggccca gggcgccgaa gccggcggcc
    10501 accacaagac acaggcgaca ctgccgctga tcccggccgt gctcgacatc acccagggcg
    10561 tggtgcccgt ggtcgccgcg ggcggtttcg cggacgggcg gggcctggcc gccgccctcg
    10621 ccctcggcgc cgacggcgtc atgatgggca cccgcttcgc cgtcaccgag gagtcgctgg
    10681 gcacgcccgg cttcaagtcc tcgctggtct ccggggccac ggcggacctc gtcgacaccc
    10741 gggccttcga cgtcgtgcgc ggcatcccgt gggacgaggt ctaccaggcg cgctcagtga
    10801 gcaacgactt cacccgcacc tggaccggcc gcgacgccga actcgccgcc cgtcgcgccg
    10861 agatcgagcc cgagtgggcc gcggcggtcg cccgcgacga cacgagccgg cgggcgctgt
    10921 tcgccggtga ggtcctcgac ctggtccacg a
//
