LOCUS       NZ_CP015098            11278 bp    DNA     linear   CON 03-MAR-2024
DEFINITION  Streptomyces qaidamensis strain S10 chromosome, complete genome.
ACCESSION   NZ_CP015098
VERSION     NZ_CP015098.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces qaidamensis
  ORGANISM  Streptomyces qaidamensis
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces; Streptomyces aurantiacus group.
REFERENCE   1  (bases 1 to 9083372)
  AUTHORS   Zhang,B.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2016) State Key Laboratory of Cryospheric
            Sciences, Chinese Academy of Sciences, Donggang West Road 320,
            Lanzhou, Gansu 730000, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SMRT Analysis v. 2.3.0
            Genome Coverage                   :: 278x
            Sequencing Technology :: Illumina HiSeq 4000; PacBio RSII
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 03/03/2024 10:55:15
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,226
            CDSs (total)                      :: 8,137
            Genes (coding)                    :: 7,871
            CDSs (with protein)               :: 7,871
            Genes (RNA)                       :: 89
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 119 of 266
            Pseudo Genes (incomplete)         :: 213 of 266
            Pseudo Genes (internal stop)      :: 25 of 266
            Pseudo Genes (multiple problems)  :: 80 of 266
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 16:26:55
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 4637520
            Orig. end                         :: 4648798
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP015098.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..11278
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="butyrolactone"
                     /region_number="12"
                     /rules="AfsA"
                     /tool="antismash"
     cand_cluster    1..11278
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="AfsA"
                     /kind="single"
                     /product="butyrolactone"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..11278
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:6278](-)"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..6278)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
     misc_feature    complement(1..14)
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 12.9"
                     /tool="antismash"
     misc_feature    complement(5..20)
                     /note="TFBS match to CRP, Cyclic AMP receptor protein,
                     confidence: medium, score: 16.52"
                     /tool="antismash"
     misc_feature    complement(52..67)
                     /note="TFBS match to CRP, Cyclic AMP receptor protein,
                     confidence: medium, score: 16.38"
                     /tool="antismash"
     gene            complement(118..1314)
                     /locus_tag="A4E84_RS20605"
                     /old_locus_tag="A4E84_20605"
     CDS             complement(118..1314)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003971741.1"
                     /locus_tag="A4E84_RS20605"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A4E84_20605"
                     /product="spore photoproduct lyase family protein"
                     /protein_id="WP_062927994.1"
                     /transl_table=11
                     /translation="MHHPSGTPGDDSDGTLFGLDALAPGPAAPGSTTAPGTDSSFRTSA
                     TARRLLSVREIYAEPAAAASPRGRQISARFPDARVIMVDSHWRIPGLHGNEGNVERWVR
                     IKGETLVLGEKKTLTTRPNGRSADWIAPGTSNGCAMACAYCYVPRRKGYANPITVFTNI
                     DRIIAHLARHIARQGPKPEPNQCDPEAWVYDIGENGDCSVDALICHNTADLVHAFRQWP
                     TAKASFATKFVNPDLLALDPRGRTRVRFSLMPPDDARLLDVRTSPVAERIAAAADFLDA
                     GYEVHFNLSPVVVRPGWQDAWAELLVHLDDVLPPRVKQQAAAEVIMLTHNRELHEVNLG
                     WHPRAEETLWRPELQQAKRSENGALNIRYRNGVKAEAVHTLRALIDHHAPWLRVRYAF"
     misc_feature    complement(1773..1786)
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 12.96"
                     /tool="antismash"
     misc_feature    complement(1776..1789)
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 12.78"
                     /tool="antismash"
     gene            1840..2613
                     /locus_tag="A4E84_RS20610"
                     /old_locus_tag="A4E84_20610"
     CDS             1840..2613
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010032569.1"
                     /locus_tag="A4E84_RS20610"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A4E84_20610"
                     /product="ScbR family autoregulator-binding transcription
                     factor"
                     /protein_id="WP_079129037.1"
                     /transl_table=11
                     /translation="MTKQDRAVRTRLALIRSAAAQFEAHGYVRAGLARISAGAGVSSGA
                     LHFHFADKAAVAEAVEDAAARALREAAVRGHGGDGCALQRLVDVTHHVARLLCADVVVR
                     AGLRLNVEGAAGRVRDLRREWRECVQGLLAEAERRGELAAGVTPQRTGAVVLAATTGIE
                     VLAREDQEWLARPSLTDLWQLLLPSLAAPRLAGAVDPAGSDPTGSGPAGAEPVGSQPVG
                     SQPVGSDPAVSDPAGRGPVARDGQAEAGRAGLALR"
     misc_feature    complement(2654..2674)
                     /note="TFBS match to MntR, Manganese transport regulator,
                     confidence: weak, score: 20.62"
                     /tool="antismash"
     gene            2733..4022
                     /locus_tag="A4E84_RS20615"
                     /old_locus_tag="A4E84_20615"
     CDS             2733..4022
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) p450"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1007: cytochrome P450"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003991559.1"
                     /locus_tag="A4E84_RS20615"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A4E84_20615"
                     /product="cytochrome P450"
                     /protein_id="WP_062927995.1"
                     /sec_met_domain="p450 (E-value: 1.4e-16, bitscore: 59.2,
                     seeds: 50, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MAQQACPFLSIDPSGQDLYGEISRIRAQGPAVEVELPGGVRAWWI
                     TGLELNKRLLAGPETSKDAFQHWPAWINGDISRTWPLAIWVSVRNMVTAYGSDHTRLRK
                     PMAAAFTKRRVDALLPRVQEIVDRALDALERIPEGEVVDLRAAFAAPIPHEVVCELFGV
                     PAGEDGPRAALYRIISRFFDTAISLEDAQANAVDLYGTLTAFLQHKREHPADDLTTALI
                     AARDEGGLSEQELMDNLILLLTAGFETTVNLIDNTVHSLLAHPGQLDLVRTGQVSWEDA
                     LEESLRFEAPGAMSGLRYAVEDIEIEPGLTIPKGDPLVVSFAGAGRDPERHGPDAERFD
                     VTRTTSRDHVSFGHGVHHCLGRPLAMAEATVALTSLFTRFPRLALADPAHPPKRLRSII
                     STGHEELPVLLHGRGPAGPRPEEAEMPAQA"
     gene            complement(4081..5004)
                     /locus_tag="A4E84_RS20620"
                     /old_locus_tag="A4E84_20620"
     CDS             complement(4081..5004)
                     /GO_function="GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /NRPS_PKS="Domain: NAD_binding_4 (51-157). E-value:
                     7.8e-08. Score: 23.7. Matches aSDomain:
                     nrpspksdomains_A4E84_RS20620_NAD_binding_4.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) RmlD_sub_bind"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1010: NAD-dependent epimerase/dehydratase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010032561.1"
                     /locus_tag="A4E84_RS20620"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A4E84_20620"
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="WP_062927996.1"
                     /sec_met_domain="RmlD_sub_bind (E-value: 5.1e-10, bitscore:
                     37.8, seeds: 37, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTVKVLITGASGFVGSRVADTAKRQPDVHVRLLSRPAPHGPEYVH
                     GDLCAPPSLRGVCADTDVLVHCATRVGGDAETVQAVNDHGTRALVEEAVRAGVRRIVYV
                     STAAVHGRGPFRGVRPGEVPIAPASPTSRTRAAAERYVLDAGGLVLRPHLVYGEGDRWV
                     IPGLVWLMKELSATLTGCEALQSMIDVDTLGRAVVAAALSPAPGGGVHHVNHPEPVAVA
                     DLLAAVSREMGEPGGGAAVDVATALDRVAGSPPALHHLGMLTVDHWFVDDAFWKDLDCP
                     PGEGFAAAFTRAAPWYRAFLDGRRAP"
     aSDomain        complement(4534..4851)
                     /aSDomain="NAD_binding_4"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_A4E84_RS20620_NAD_binding_4.1"
                     /evalue="7.80E-08"
                     /label="A4E84_RS20620_NAD_binding_4.1"
                     /locus_tag="A4E84_RS20620"
                     /protein_end="157"
                     /protein_start="51"
                     /score="23.7"
                     /tool="antismash"
                     /translation="PSLRGVCADTDVLVHCATRVGGDAETVQAVNDHGTRALVEEAVRA
                     GVRRIVYVSTAAVHGRGPFRGVRPGEVPIAPASPTSRTRAAAERYVLDAGGLVLRPHLV
                     YG"
     gene            complement(5001..6278)
                     /locus_tag="A4E84_RS44620"
                     /old_locus_tag="A4E84_20625"
     CDS             complement(5001..6278)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     butyrolactone: AfsA"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010032560.1"
                     /locus_tag="A4E84_RS44620"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A4E84_20625"
                     /product="ScbA/BarX family gamma-butyrolactone biosynthesis
                     protein"
                     /protein_id="WP_237304966.1"
                     /sec_met_domain="AfsA (E-value: 4.3e-21, bitscore: 73.6,
                     seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSALTEDQTGRGGRPANGTATTVENPPHPAVPRQTAPPSPATAEP
                     APPTTASKEPASSATVFEDPAPSTTAPEKPASSATTSEDPASSTTAPEKPASLATASEE
                     PAPPSPATDQPAPHSPVTAVTAVTAVTTELAHRTTETDVFPTRWTRISDTRFRFTAHWP
                     ATHPFFGPVDDRHQDPMIVGETLRQASMVLAHAEFGAPADTHFVMWDLTVSVDPTALLL
                     ADAAEPVEFDVVCSEIRRRGHGLRSMRTTMEFRRAGRFVARGTGSTGCTSPRAYRRLRA
                     GRLDSLNTPVPLLAGIAPQAAGRSRAEDVVLAPADRPGVWNLRVDTGHPVLFPRPNDHV
                     PGMVLFEAARQAATAASGLRPFLPGTLTAEFAHYAELHSPCRIETEVFDERPGEVTVRV
                     SGRQDGASVFTASLTSARPARVRSLS"
     misc_feature    complement(5985..5987)
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     misc_feature    complement(6264..6279)
                     /note="TFBS match to CRP, Cyclic AMP receptor protein,
                     confidence: weak, score: 16.04"
                     /tool="antismash"
     misc_feature    complement(6359..6372)
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 13.06"
                     /tool="antismash"
     misc_feature    complement(6360..6373)
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 13.63"
                     /tool="antismash"
     misc_feature    complement(6361..6374)
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 13.64"
                     /tool="antismash"
     gene            6456..7172
                     /locus_tag="A4E84_RS20630"
                     /old_locus_tag="A4E84_20630"
     CDS             6456..7172
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1057: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018092050.1"
                     /locus_tag="A4E84_RS20630"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A4E84_20630"
                     /product="ScbR family autoregulator-binding transcription
                     factor"
                     /protein_id="WP_062927997.1"
                     /transl_table=11
                     /translation="MARQERAIRTRRTILEAAAAVFDERGYTSATIGEILDRAGVTKGA
                     LYFHFGSKEELAVGVFEEQLAIALPPQSSKLQELVDSGLVMARRLRSEPLVRASVGLAL
                     DQGAMDLDRTPAFRMWIDQNTQLLTEAKARGELLQHVDVAETAELLVGSFSGVQLLSQL
                     RCQRQDLERRVVVLFEHFLPSITVPAVLARLDISVDRAERLLAQVRTPEEAAVTVEAPV
                     PSVPSVPSAPSVPSGA"
     gene            7307..8746
                     /locus_tag="A4E84_RS20635"
                     /old_locus_tag="A4E84_20635"
     CDS             7307..8746
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010032556.1"
                     /locus_tag="A4E84_RS20635"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A4E84_20635"
                     /product="DUF2252 domain-containing protein"
                     /protein_id="WP_062927998.1"
                     /transl_table=11
                     /translation="MTRAGAQDEGGAKAREGAPRRLPEVRGFAEWPAAGSPKDEGKALR
                     KRIPRSAHAMLDLDASRPDAVSAVEESGRGRLPELAPIRVGRMTATPFAFLRGAAGLMA
                     YDLARTPMTGIRAQICGDAHAANFGLYGDARGGLVIDLNDFDETVSGPWEWDLKRLATS
                     LVLAGRVAGADEDECRKAAYDAAGAYRRTMRLLARLPVLDAWNAIADEELVSHADAHDL
                     LGTLERVSEKARHNTSGRFAAKSTETTEDGGRRFVDAPPVLRRVPDAEAAAVAASLEEY
                     AGTLQEDRLPLLARYAVHDVAFRVVGTGSVGTRSYVVLLLDHRGEPLVLQVKEARASAL
                     VPHLVKAGFEAPEAGHEGRRVVMGQKRMQVVSDNLLGWTTVDGFPFQVRQFRNRKGSVD
                     PAALAADQMDDYGRMTGALLARAHAHSADPRLIAGYCGKNEELDEAMADFAVAYADRTE
                     ADHAELVAGVRAGKVAAEVGV"
     gene            9062..9907
                     /locus_tag="A4E84_RS20640"
                     /old_locus_tag="A4E84_20640"
     CDS             9062..9907
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003991564.1"
                     /locus_tag="A4E84_RS20640"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A4E84_20640"
                     /product="hypothetical protein"
                     /protein_id="WP_062927999.1"
                     /transl_table=11
                     /translation="MTTPEAEQDGGDAARPEARLERAVRAAEQALIEYEIAVETFRVEV
                     ENFSRLHEQKLGPIYARLEELDARILEARAARSGDPADRQRAEEARARLAPIPGVEDLL
                     HGWMDGSGLFPEASAMLTDQPVRPPQRVRPSEEARKLYRDLARKAHPDLAQEEKERARR
                     EEFITRVNAAYARGDEAELRELAAEWAKGPELKRGPSRAEELYARLEWLAQRKELLGLL
                     ARELEDSAIGHMLQLAPDDPDRLLDEIGQGLLAQVTEREMELAALLGEGEGQGRGESQV
                     "
     gene            9955..10302
                     /locus_tag="A4E84_RS20645"
                     /old_locus_tag="A4E84_20645"
     CDS             9955..10302
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1163: rhodanese domain-containing protein"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015659144.1"
                     /locus_tag="A4E84_RS20645"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A4E84_20645"
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="WP_062928000.1"
                     /transl_table=11
                     /translation="MSFGAGVPTVAVADLKDGDFLLDVREDDEWKAGHAEGALHIPISE
                     FVARYGELTEAAPQDGRVHVICRSGGRSAQVTMYLAQQGVDAVNVDGGMQVWEAAGRPV
                     VTDDGQEGFVL"
     misc_feature    complement(10813..10828)
                     /note="TFBS match to CopR, Copper-responsive regulator,
                     confidence: weak, score: 16.64"
                     /tool="antismash"
ORIGIN
        1 cctttgtgaa acatcacaca gcccggccag gtcccacggc aggccccggc gcgtgagcta
       61 actcacgggc ggggcctgcc gtcttcggtg aggtcggcgc ggcgcagctc ctggggttca
      121 gaaggcgtag cgcacgcgta gccagggcgc gtggtggtcg atcagggcgc gcagggtgtg
      181 cacggcctcc gccttgaccc cgttgcggta gcggatgttc agggcgccgt tctcggagcg
      241 cttggcctgc tggagctcag ggcgccacag cgtctcctcg gcgcggggat gccagccgag
      301 gttgacctcg tgcagctccc ggttgtgggt gagcatgatc acctccgcgg cggcctgctg
      361 tttcacccgc gggggcagga cgtcgtcgag gtggacgagc agctcggccc aggcatcctg
      421 ccagccgggg cggacgacca cgggtgagag gttgaagtgc acctcgtatc ccgcgtccag
      481 gaagtccgcc gcggcggcga tgcgctcggc gacgggtgag gtgcgcacat cgagtagccg
      541 ggcgtcgtcc ggtggcatca gcgagaaccg gacgcgggtg cgaccgcgtg ggtcgagggc
      601 cagcaggtcg gggttgacga acttggtggc gaaggacgcc ttggcggtgg gccactgccg
      661 gaaggcgtgc accaggtcag cggtgttgtg gcagatcagg gcgtccaccg agcagtcgcc
      721 gttctcgccg atgtcgtaca cccacgcctc ggggtcgcac tggttcggct cgggcttggg
      781 cccctgtcga gcgatgtgcc tcgcgaggtg ggcgatgatc cggtcgatgt tggtgaagac
      841 ggtgatgggg ttggcgtatc ccttgcgccg gggcacgtag cagtaggcgc aggccatcgc
      901 gcagccgttg gaggtgccgg gagcgatcca gtccgccgac cggccgttgg ggcgggtggt
      961 gagggtcttc ttctcgccca ggaccagcgt ctctcccttg atgcgcaccc agcgctcgac
     1021 gttgccctcg ttgccgtgca gcccggggat gcgccagtgg gagtcgacca tgatcacccg
     1081 cgcgtcgggg aaccgggcgc tgatctgccg ccctcgcggg gacgcggcgg ccgcgggctc
     1141 ggcgtagatc tcccgcacgg acagcagccg acgggccgtg gcggaggtcc ggaaggacga
     1201 gtccgtgccg ggagcggtgg tggagccagg ggcggcgggg ccgggggcga gggcgtcgag
     1261 cccgaacagt gtgccgtcgg agtcgtcgcc cggggtgccg gacgggtggt gcatgagtgc
     1321 tcccgtacgg atgcgtgcga ggtggggggt gcgaggtggg gggggcgacg cgggaaggtc
     1381 cgcccgtcac ccgggagggg ccggtgggtg ggttgggcgc ttgttccgac gggtggtgcc
     1441 gccgatcgcc acggagctga ggcgctcgtg gcacggggcc ggcatctggt ggtcatcgcg
     1501 gggcgatcag cgtgatcgtc cgtctcggtg gccgcctcgg gttccgcctg gcggcggtgg
     1561 ccaccggtgt gcgggcgggc ctggcgtcca gtctaccggc gctccgctgg tggagccggg
     1621 gcggcgaggg cggcgtgggc ggcgtgggcg gcgcgagcgg cgtgggcggc gtgggcggcg
     1681 cgagcggcgt gggcgtctgt gggcgtgccc tgggcctgga aggggagtgg ccggggcggg
     1741 cagcagggca agggccgctg ttgagtctcg acgcgttgta taaaaaccag cgagttggtt
     1801 tcattgcgtc gccatcagag ccctgcaggg gagccacggg tgaccaaaca ggaccgggcc
     1861 gtccggaccc gtctcgcgct gatccggtcg gcggccgcac agttcgaggc ccatgggtac
     1921 gtaagggccg gcctggcgcg gatcagcgcg ggagccggcg tcagctccgg tgcgctgcac
     1981 ttccacttcg ccgacaaggc cgccgtcgcg gaggccgtcg aggacgctgc cgcccgagcc
     2041 ctgcgcgagg ccgcggtccg tggtcacggt ggtgacggct gcgccctgca acgtctcgtg
     2101 gacgtcacgc atcacgtcgc ccggctcctg tgcgccgatg tcgtggtgcg tgcggggctg
     2161 cggctgaacg tggaaggtgc ggccgggcgg gtgcgggacc tgcgccggga gtggcgggag
     2221 tgcgtgcagg ggctgctggc cgaggcggag cgcagggggg aactcgccgc cggtgtgacg
     2281 ccgcagcgca ccggcgccgt cgtcctcgcc gccacgaccg gcatcgaggt gctggcgcgg
     2341 gaggaccagg agtggctcgc ccggccgtcg ctgaccgacc tgtggcagtt gctcctgccg
     2401 agcctggcgg cgcctcggct ggccggggcg gtggatccgg cggggagtga tccgacgggg
     2461 agcggccctg cgggggccga accggtgggg agtcaaccgg tggggagtca accggtgggg
     2521 agtgatccgg cggtgagtga tccggcgggg cgcggaccgg tcgcccggga cgggcaggcg
     2581 gaggcgggca gggcggggct cgcgctgcgg tagccgcccc ccttcggccg acagtgggtg
     2641 aagctgtggc caaaacaaac cggatgccaa gttttttccg tccgcggatc gcacgatggt
     2701 cccccagcct ctggaccaag gagcgatgag tcatggcaca gcaggcctgc cccttcctgt
     2761 cgatcgaccc gtccggccag gatctctatg gcgagatatc ccgcatccgc gcccagggcc
     2821 cggccgtgga ggtcgaactc cccggcggcg tccgggcgtg gtggatcacc ggcctggagc
     2881 tgaacaagcg gctcctggcc ggaccggaga cgagcaagga cgccttccag cactggccgg
     2941 cctggatcaa cggggacatc tcccgcacct ggccgctcgc catctgggtc tcggtgcgca
     3001 acatggtcac cgcctacggc tccgaccaca cccggctgcg caagcccatg gccgccgcct
     3061 tcaccaagcg ccgcgtcgac gcgctgctgc cgcgtgtcca ggagatcgtc gaccgggcgc
     3121 tcgacgccct ggagcggatc ccggagggcg aagtcgtgga cctgagggcg gcgttcgcgg
     3181 cgccgatccc gcacgaggtg gtgtgcgagc tcttcggcgt accggccggc gaggacggcc
     3241 cgcgcgccgc cctctaccgc atcatcagcc gcttcttcga cacggcgatc tccctggagg
     3301 acgcccaggc caacgccgtc gatctctacg gcacgctgac cgcgttcctc cagcacaagc
     3361 gggagcaccc cgccgacgac ctgacgacgg cgctcatcgc cgcgcgcgac gaaggcggcc
     3421 tcagcgaaca ggagttgatg gacaacctga tcctgctgct caccgccggt ttcgagacga
     3481 ccgtcaacct catcgacaac accgtgcaca gcctgctcgc ccaccccggc cagctcgacc
     3541 tcgtccgcac cgggcaggtc agctgggagg acgcactgga ggagtcgttg cgcttcgagg
     3601 cgccgggcgc catgtcgggc ctgcgctacg ccgtcgagga catcgagatc gagccgggtc
     3661 tgaccatccc caagggtgac ccgctcgtgg tctccttcgc cggcgccggg cgcgaccccg
     3721 agcggcacgg gccggacgcc gaacggttcg acgtgacgcg cacaaccagc cgtgaccacg
     3781 tgtccttcgg ccacggcgtg caccactgcc tgggccggcc cctcgcgatg gccgaggcga
     3841 ccgtggctct gacgtcgctg ttcacgcgct tcccgcggct ggcactggcc gatccggcgc
     3901 atccgccgaa gcggctgcgg tccatcatct ccaccggcca cgaggaactg ccggtgctgc
     3961 tgcacggccg gggcccggcg ggcccgcgcc cggaggaggc cgagatgccg gcgcaggcgt
     4021 aggcggaggc ggcggccgag atgccggcgt aggcggagat gccggcggag gcgggggcgg
     4081 tcagggggcc ctccggccgt cgaggaacgc gcggtaccag ggcgccgcgc gggtgaacgc
     4141 ggccgcgaac ccctcaccgg gcgggcagtc gaggtccttc cagaacgcgt cgtccacgaa
     4201 ccagtggtcg acggtgagca tgccgaggtg atggagggcg ggcggtgacc cggcgacccg
     4261 gtcgagggcg gtggcgacgt ccacggccgc accgccgccg ggctccccca tctcccggga
     4321 cacggcggcc agcaggtccg cgaccgccac cggctcgggg tggttgacgt ggtgcacacc
     4381 gccgccgggc gcgggggaga gcgcggcggc caccaccgca cggcccaggg tgtcgacgtc
     4441 gatcatcgac tggagcgcct cgcagccggt cagggtggcc gacagttcct tcatcagcca
     4501 caccaggccg gggatcaccc agcggtcgcc ctcgccgtac acgaggtgcg ggcgcagcac
     4561 gaggccgccg gcgtcgagga cgtaccgttc ggcggcggcc cgggtgcggc tggtgggtga
     4621 cgccggagcg atcggcacct ccccggggcg tacgccgcgg aaggggcccc ggccgtggac
     4681 ggcggccgta ctgacgtaca cgatccggcg caccccggcg cgcacggcct cctcgaccag
     4741 ggcacgggtg ccgtggtcgt tgaccgcctg gacggtttcg gcgtcgccgc cgacccgcgt
     4801 ggcgcagtgc accaggacgt cggtgtcggc gcagaccccg cgcagggagg gcggggcgca
     4861 gaggtcgccg tggacgtact cggggccgtg cggcgcaggc cgggacagca ggcgtacgtg
     4921 gacgtccggt tgccgctttg ccgtgtcggc gacccggctg ccgacgaagc cgctcgcgcc
     4981 cgtgatcaga accttgaccg tcacgacagg gaccgtaccc gcgccggccg ggcggaggtc
     5041 aggctcgcgg tgaagacgga cgcgccgtcc tgccgccccg agacccgtac ggtcacctcc
     5101 ccggggcgct cgtcgaagac ctcggtctcg atccggcacg ggctgtgcag ctctgcgtag
     5161 tgggcgaact cggccgtcag cgtgcccggc aggaaggggc gcaggccgga cgcggcggtc
     5221 gcggcctggc gggccgcctc gaacaggacc atgccgggga cgtggtcgtt gggccgtggg
     5281 aagaggaccg ggtggccggt gtcgacgcgc aggttccaga cgccgggacg gtcggcgggg
     5341 gcgaggacga cgtcctcggc acgggaccgt cccgcggcct gcggcgctat gcccgccagg
     5401 agcggaaccg gtgtgttcag ggaatccaac cggccagcgc gcaggcggcg gtaggcgcgc
     5461 ggtgaggtgc agccggtgct tccggtgccg cgggcgacga accgacccgc gcggcggaac
     5521 tccatcgtcg tccgcatgct gcgcagtccg tggccgcggc ggcggatctc ggagcagacg
     5581 acgtcgaact cgaccggctc ggcggcgtcc gccagcagca gtgcggtcgg gtcgacgctg
     5641 accgtcaggt cccacatgac gaagtgggtg tcggccgggg cgccgaactc ggcgtgcgcc
     5701 agcaccatcg aagcctgccg caacgtttcg cccacgatca tcgggtcctg gtgccggtcg
     5761 tcgacggggc cgaagaaggg gtgggtggcc ggccagtgcg cggtgaaccg gaagcgggtg
     5821 tcggagattc gggtccaccg ggtggggaag acgtccgtct cggtggtgcg gtgggcgagt
     5881 tccgtggtga cggcggtgac ggcggtgacg gcggtgacgg gcgagtgggg ggcgggctgg
     5941 tcggtggcgg gagacggcgg cgcgggttct tcggaggcgg tggctaagga tgcgggtttc
     6001 tcgggggcgg ttgtcgagga agcggggtct tcggaggtgg tggccgagga tgcgggtttc
     6061 tcgggggcgg ttgtcgaggg ggcggggtct tcgaagacgg tggccgagga cgcgggttcc
     6121 ttggaggcgg tcgtcggggg tgcgggttcc gcggttgcgg gactcggcgg tgccgtctgc
     6181 cgggggacgg cggggtgtgg cgggttctcg acggtcgtag cggttccgtt tgcgggacgg
     6241 cccccgcgtc cggtctgatc ctccgtgagt gcgctcaaag gtcctgtctg tggcatgaga
     6301 acccccagga tcggctgcct ggccgcgatc gacgcttgac taagatacgg acgagccggt
     6361 ttttttcaaa gggtgagcca agccgctggg acaggccctc gcgccctcgc cacacagcga
     6421 cttcacaggg atcgaccaag ctcaggaggc acccgatggc gcgacaggag cgcgcgatcc
     6481 gcacccggcg gaccatcctg gaggcggccg cggccgtctt cgacgaacgc ggctacacct
     6541 cggcgaccat cggcgagatc ctggaccggg caggcgtcac caagggcgcg ctgtacttcc
     6601 acttcggctc caaggaggag ctggcggtgg gggtgttcga ggagcagctc gcgatcgcgc
     6661 tcccgccgca gtccagcaag ctccaggaac tcgtcgactc cggcctggtg atggcccgcc
     6721 ggctgcggtc cgaaccgctg gtgcgggcca gtgtggggct cgccctcgac cagggggcca
     6781 tggacctcga ccgcaccccg gccttccgga tgtggatcga ccagaacaca cagttgctga
     6841 ccgaggcgaa ggcgcgcggc gagctgctgc agcacgtcga cgtggcggag acggcggagc
     6901 tgctcgtcgg cagcttctcc ggggtgcagc tgctgtccca gctgcgttgc cagcggcagg
     6961 atctggagcg ccgcgtcgtc gtgctgttcg agcacttcct gcccagtatc accgtgcccg
     7021 cggtgctggc caggctcgac atctcggtgg accgggccga gcggttgctg gcgcaggtgc
     7081 ggacgccgga ggaagcggcc gtgaccgtgg aggctccggt gccttcagtg ccttcagtgc
     7141 cttcagcgcc ttcggtgccc tcgggggcct agcgcaccgc cgccgtaccg cccggcacgg
     7201 attgtccaca gctgtggagt tgtccacagg gtctgacgcg tttcggccgc cggttgtacg
     7261 gtcggcacga gttgatgttc gtgcgcgtgc gggggaggcg gtcgggatga ccagggctgg
     7321 agctcaggac gagggcgggg ccaaggcgag ggaaggggcg ccacgccggc tgcccgaggt
     7381 gcgtggcttc gccgaatggc cggccgcggg ctcgcccaag gacgagggca aggccctgcg
     7441 caagcggatc ccgcgcagcg cgcacgccat gctcgacctg gacgcgtccc ggcccgacgc
     7501 ggtgagcgcc gtcgaggagt ccggccgcgg gcggctgcct gagctggccc cgatccgggt
     7561 cggccgcatg accgccaccc cgttcgcgtt cctgcgcggc gcggcgggcc tcatggcgta
     7621 cgacctggcg cgcacgccca tgaccggcat ccgggcccag atctgcggcg acgcccacgc
     7681 ggccaatttc ggcctgtacg gcgacgcgcg cggcggcctg gtcatcgacc tgaacgactt
     7741 cgacgaaacg gtgtccggcc cctgggagtg ggacctcaag cgtctcgcga cctccctggt
     7801 gctcgcgggc cgagtggcgg gcgccgacga ggacgagtgc cgcaaggcgg cgtacgacgc
     7861 ggcgggcgcc taccgcagga ccatgcggct cctggcccgg ctcccggtgc tggacgcgtg
     7921 gaacgccatc gcggacgagg aactcgtctc ccacgcggac gcccatgatc tgctcggcac
     7981 gctggagcgg gtctccgaga aggcccgtca caacaccagc ggccgcttcg cggccaagtc
     8041 gaccgagacc accgaagacg gcgggcgccg cttcgtcgac gccccgccgg tgctgcgacg
     8101 ggtcccggac gccgaggccg cggcggtggc ggcgtccctg gaggagtacg ccggcaccct
     8161 gcaggaggac cgcctgccgc tcctcgcccg gtacgcggtg cacgacgtgg ccttccgggt
     8221 cgtcggcacg ggcagtgtcg gcacccggtc ctacgtcgtg ctgctcctcg accaccgggg
     8281 cgagccgctg gttctccagg tgaaggaggc acgggcgtcg gcgctggtgc cgcatctggt
     8341 gaaggccggt ttcgaggcgc cggaggccgg tcacgaaggg cggcgggtcg tgatgggcca
     8401 gaagcggatg caggtcgtga gcgacaacct gctgggctgg acgacggtcg acggtttccc
     8461 cttccaggtc cgccagttcc gcaaccgcaa gggcagcgtc gacccggccg cgctggccgc
     8521 cgaccagatg gacgactacg gccgcatgac cggtgcgctc ctggcccgcg cacacgcgca
     8581 cagcgccgat ccgcggctca tcgccgggta ctgcgggaag aacgaggaac tggacgaggc
     8641 gatggcggac ttcgccgtgg cctacgcgga ccggacggag gcggatcacg cggagctggt
     8701 ggccggggta cgggcgggga aggtcgcggc agaggtgggg gtgtagaggc tgcgtgtttt
     8761 cgggtgcgtg gcgcgggatg cggggtgccg ggtgccgggt gccgggtgcg cgctgagggc
     8821 tgagggtgcg tgcggctgcg gctgcggctg cggctgcggg tggcgcgttg cgggttctgt
     8881 gagactgcgg gttgtgggct gtggctgcgg gatcgccgga tggggctgat ggctgtggct
     8941 cgtgggacgt cggatggggc gtcggatggg gcgtcggatg gggcgccgtc cgccgggtcc
     9001 gtccgaccgg ggctgccctc cggggcgtgg acggggtcgg gccgtcacct aggctggtcg
     9061 ggtgacgacc ccggaagctg agcaggatgg tggcgacgcc gcccggcccg aggcgcggct
     9121 ggagcgggcc gtgcgggccg ccgagcaggc gttgatcgag tacgagatcg cggtggagac
     9181 cttccgcgtc gaggtcgaga atttctcccg gctgcacgaa cagaagctcg gcccgatcta
     9241 cgcccggctc gaggagctgg acgcccggat cctggaagcc agagccgccc gcagcggcga
     9301 ccccgcggac cggcagcggg cggaggaggc cagggcgcgg ctcgccccaa ttcccggcgt
     9361 cgaggatctg ctgcacggct ggatggacgg cagcggtctg ttcccagagg cctccgcgat
     9421 gctcacggac cagccggtcc ggccaccgca gcgggtccgc cccagcgagg aggcccgcaa
     9481 gctgtaccgc gaccttgccc gcaaggccca cccggacctg gcgcaggagg agaaggaacg
     9541 cgcgcggcgc gaggagttca tcacgcgggt caacgccgcc tacgcccggg gcgacgaggc
     9601 cgagctgcgg gaactcgccg cggaatgggc caagggaccc gagctgaagc ggggcccgag
     9661 ccgtgccgag gagctctacg cccggctcga atggctcgcc cagcgcaagg agctgctcgg
     9721 cctgctcgcc agggagctgg aggacagcgc catcggccac atgctccagc tggccccgga
     9781 cgacccggac cggctcctgg acgagatcgg gcaagggctc ctcgcccagg tcaccgagcg
     9841 cgagatggag ctggccgcgc tgctcggcga aggagaaggc cagggccggg gcgagagcca
     9901 ggtctgagcc gtgccccggc cggggtccgg gccggttccg gtagcgtcgg acgcatgagt
     9961 tttggagctg gtgtacccac ggtcgcggtc gcggacctca aggacggtga cttcctgctg
    10021 gacgtccggg aggacgacga gtggaaggcc ggtcacgccg aaggggcgct gcacatcccc
    10081 atcagcgagt tcgtcgcccg ctacggcgag ctgaccgagg ccgccccgca ggacggccgg
    10141 gtccatgtga tctgccgctc cggaggccgg tccgcccagg tcacgatgta tctggcccag
    10201 cagggcgtcg acgccgtgaa cgtcgacggc ggtatgcagg tgtgggaggc ggcgggccgt
    10261 cccgtcgtga cggacgacgg gcaggaaggg ttcgtcctct agaccgcgtg ggccgcgtgg
    10321 gccgcgtggg ccgcgtgggc ccgtgggccg catgggctct gcatgggccg catgggccac
    10381 ctgaccgact aggtcgcccg ggccgttgga ctgcctggac cgcccaggcc gccggggccg
    10441 cccgttctcg tcaggccagt aggtgggtcg ccaggaggtc gcccagggcc tcttcgtggg
    10501 ccgaggccgg gccgagtgac agttccaggt gcttggccca ggcgtggtag cggtgcaggg
    10561 ggtagtcgac gtcggcgcca aagccgccgt gcaggtgctg cgcggtctgc acgacccggc
    10621 gtacgccctc cgccgcccag atcttggcga cggccacgtc tccggcggcg ggcagcgggc
    10681 ccggggcccc ggaggagatc cgccacgcgg cctgccacag cgtggcctcc atggcgcgca
    10741 ggtcgatata gcggtcggcg gcctgcacgg cgacggcctg gaaggtcgcg atggggaaac
    10801 cgaactgctc acgcttgcct gtgtagtccc cggtcatccg cagcacacgc tccccgaggc
    10861 cgagcgccag cgcacacgtc ccggtggcca gcaggtcccg cagccactcc cacgccccgt
    10921 cggtgtcgat gacgtcccgg gcggcgagcc gtgccgactc cagccgcagt tcgccgagcc
    10981 gctcgccgct ggtggagaac tgctcggcga gggcggttcc ctcgtggccg cgcggcacca
    11041 gggccagcac ggtccgtccg gtcccggtgt gcgcggggac gagcgtgacg tccgcgtcgt
    11101 gggcccacgg caccgcggtc tggaccccgt cgagcaccca ggtgccgccg tcctgccggg
    11161 cggtcacggc ctgctcggcc gggtcgtggc cggtgcggcc gtgcgcggcg acggtcagca
    11221 cggtctcgcc cctgccggcc cgggcgagca ggcccctctt cgcctcttcg ccgccgta
//
