LOCUS       NZ_CP015098            10480 bp    DNA     linear   CON 03-MAR-2024
DEFINITION  Streptomyces qaidamensis strain S10 chromosome, complete genome.
ACCESSION   NZ_CP015098
VERSION     NZ_CP015098.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces qaidamensis
  ORGANISM  Streptomyces qaidamensis
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces; Streptomyces aurantiacus group.
REFERENCE   1  (bases 1 to 9083372)
  AUTHORS   Zhang,B.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2016) State Key Laboratory of Cryospheric
            Sciences, Chinese Academy of Sciences, Donggang West Road 320,
            Lanzhou, Gansu 730000, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SMRT Analysis v. 2.3.0
            Genome Coverage                   :: 278x
            Sequencing Technology :: Illumina HiSeq 4000; PacBio RSII
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 03/03/2024 10:55:15
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,226
            CDSs (total)                      :: 8,137
            Genes (coding)                    :: 7,871
            CDSs (with protein)               :: 7,871
            Genes (RNA)                       :: 89
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 119 of 266
            Pseudo Genes (incomplete)         :: 213 of 266
            Pseudo Genes (internal stop)      :: 25 of 266
            Pseudo Genes (multiple problems)  :: 80 of 266
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 16:26:55
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 3247944
            Orig. end                         :: 3258424
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP015098.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10480
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="melanin"
                     /region_number="9"
                     /rules="melC"
                     /tool="antismash"
     cand_cluster    1..10480
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="melC"
                     /kind="single"
                     /product="melanin"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10480
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5480](-)"
                     /cutoff="20000"
                     /detection_rule="melC"
                     /neighbourhood="5000"
                     /product="melanin"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5480)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="melC"
                     /neighbourhood="5000"
                     /product="melanin"
                     /protocluster_number="1"
     gene            complement(379..1296)
                     /locus_tag="A4E84_RS14170"
                     /old_locus_tag="A4E84_14170"
     CDS             complement(379..1296)
                     /NRPS_PKS="Domain: Abhydrolase_1 (69-289). E-value:
                     7.4e-20. Score: 63.5. Matches aSDomain:
                     nrpspksdomains_A4E84_RS14170_Abhydrolase_1.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF00561"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Abhydrolase_6"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1036: alpha/beta hydrolase fold protein"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010472070.1"
                     /locus_tag="A4E84_RS14170"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A4E84_14170"
                     /product="alpha/beta fold hydrolase"
                     /protein_id="WP_062926916.1"
                     /sec_met_domain="PF00561 (E-value: 1.3e-13, bitscore: 50.1,
                     seeds: 48, tool: rule-based-clusters)"
                     /sec_met_domain="Abhydrolase_6 (E-value: 1.5e-20, bitscore:
                     73.6, seeds: 455, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MAVSETTARTQADIGRYVSDTWRDRYFAACDAVYALGPPALAEQD
                     VETKFGTTHVYRYGPTDKAAQSRTPVVLVHGAGSCSAMWYPNTPALSADRPVYAVDTPG
                     DPGRSVQREPIHQPERAAQWLDETLDGLGLERVHLVGTSYGGWLALNQAHRRPERLASV
                     TLLDPGGLEKVGLRFFVWIFAGLFATFAPRKLRPRLAAWLEQPVLVMPELRTMIRTAIR
                     AYRIRRPAPLPLTEAELSGIRTPLYLVLGKRSLLLHPERQMERVPRLIPGARAEIITGT
                     GHGPQIDHAQEMNRRMLAFMASVD"
     aSDomain        complement(430..1089)
                     /aSDomain="Abhydrolase_1"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_A4E84_RS14170_Abhydrolase_1.1"
                     /evalue="7.40E-20"
                     /label="A4E84_RS14170_Abhydrolase_1.1"
                     /locus_tag="A4E84_RS14170"
                     /protein_end="289"
                     /protein_start="69"
                     /score="63.5"
                     /tool="antismash"
                     /translation="PVVLVHGAGSCSAMWYPNTPALSADRPVYAVDTPGDPGRSVQREP
                     IHQPERAAQWLDETLDGLGLERVHLVGTSYGGWLALNQAHRRPERLASVTLLDPGGLEK
                     VGLRFFVWIFAGLFATFAPRKLRPRLAAWLEQPVLVMPELRTMIRTAIRAYRIRRPAPL
                     PLTEAELSGIRTPLYLVLGKRSLLLHPERQMERVPRLIPGARAEIITGTGHGPQIDH"
     CDS_motif       complement(835..888)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_A4E84_RS14170_0001"
                     /evalue="8.00E-04"
                     /label="NRPS-te1"
                     /locus_tag="A4E84_RS14170"
                     /protein_end="154"
                     /protein_start="136"
                     /score="12.0"
                     /tool="antismash"
                     /translation="VHLVGTSYGGWLALNQAH"
     gene            1401..2018
                     /locus_tag="A4E84_RS14175"
                     /old_locus_tag="A4E84_14175"
     CDS             1401..2018
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1057: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018560477.1"
                     /locus_tag="A4E84_RS14175"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A4E84_14175"
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="WP_062926917.1"
                     /transl_table=11
                     /translation="MPKRVDHAERRTEIAEALVRVAGRRGLHAVGMRDVAAEAGVSLRL
                     VQYYFETKEKLLLFGLEHLTGKFGERLAARIRAAGDEPGPRAMVEALLMTALPTDEESR
                     IFHHLYTSYAVLAVTDRTLAAQPFIKDPDAAEGALTDILRQAQTADLLQPGVDPQLTAA
                     GLLAMSAGLGTSILVGQRSPESASAVLDDHLDRVFRQTGTAG"
     gene            2299..2610
                     /locus_tag="A4E84_RS14180"
                     /old_locus_tag="A4E84_14180"
     CDS             2299..2610
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="A4E84_RS14180"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="A4E84_14180"
                     /product="hypothetical protein"
                     /protein_id="WP_159029574.1"
                     /transl_table=11
                     /translation="MPVREAVLTCGVALPLAAAVCGSVLVPLSIALTGGSAVPALADGP
                     LSALAAGALVLTAGTVALAGLLALWSAAAMPGGGEGRFLTTVPGRHFPHPRRTRDHGR"
     gene            2642..3601
                     /locus_tag="A4E84_RS14185"
                     /old_locus_tag="A4E84_14185"
     CDS             2642..3601
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) 2-Hacid_dh_C"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1072: dehydrogenase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014672797.1"
                     /locus_tag="A4E84_RS14185"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A4E84_14185"
                     /product="D-2-hydroxyacid dehydrogenase family protein"
                     /protein_id="WP_062926919.1"
                     /sec_met_domain="2-Hacid_dh_C (E-value: 3.5e-51, bitscore:
                     171.9, seeds: 135, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRLRCAVLDDFQQVAAEAADWSVVADDVEVVFFDRHIDGEDDLAA
                     ALAGFDIVVTLRERVPFPGSLITRLPRLKLIVASGMRNSVIDYAAAEEQGVTVCGTASS
                     STPPVELTWALLLGLARGIVEESNALRSGGPWQSTVGADLHGRRLGLLGLGKIGGRVAR
                     VGLAFGMHVSAWSQNLTRERTGEVGVERAGSKEELLAGSDFVSVHLALGDRTRGLLGPA
                     ELALLKPTAYLINTSRAAIVDQDALLDALHTGRIAGAGVDVFDIEPLPADHPMRTAPRL
                     LATPHLGYVSRANYATYYGHAVEDIRAFLDGTPVRRLP"
     gene            3762..4043
                     /locus_tag="A4E84_RS14190"
                     /old_locus_tag="A4E84_14190"
     CDS             3762..4043
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020115467.1"
                     /locus_tag="A4E84_RS14190"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A4E84_14190"
                     /product="chaplin"
                     /protein_id="WP_062926920.1"
                     /transl_table=11
                     /translation="MSRIAKAAGAALGTGAVVLSGAGLAVADAGAQGAAVGSPGVLSGN
                     VVQVPVHIPVNLCGNTVNVIGLLNPAFGNECENKDDPKSHPGGYGYGH"
     gene            complement(4151..5017)
                     /locus_tag="A4E84_RS14195"
                     /old_locus_tag="A4E84_14195"
     CDS             complement(4151..5017)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020116480.1"
                     /locus_tag="A4E84_RS14195"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A4E84_14195"
                     /product="tyrosinase family protein"
                     /protein_id="WP_062926921.1"
                     /transl_table=11
                     /translation="MAYTRKDVSTLTRTEKRRFVKAMLEVKRRGEYDEFVRTHIEYYTS
                     DGEDGLRTAHMAPSFLPWHRQFLLDLEKALRRVDASVSVPYWDWTRDRTTTSAPWTADL
                     LGGTGRRSDRQVMTGPFAYAEGNWTIKEGITDEEFLTRDLGRARDPIQLPTEAELERAL
                     KDPLYDASPWDSTTGKGFRNKLEGWGSSQGSASWQNHNRVHRWVGGAMVGGASVNDPVF
                     WMHHAFVDLVWSRWQRRHREHRYLPAKPPGPGSAQYRRVVARHEKLPPWNVTPDQLEDH
                     GRIYRYG"
     gene            complement(5001..5480)
                     /locus_tag="A4E84_RS14200"
                     /old_locus_tag="A4E84_14200"
     CDS             complement(5001..5480)
                     /GO_function="GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /GO_process="GO:0042438 - melanin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     melanin: melC"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006378445.1"
                     /locus_tag="A4E84_RS14200"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A4E84_14200"
                     /product="tyrosinase family oxidase copper chaperone"
                     /protein_id="WP_062926922.1"
                     /sec_met_domain="melC (E-value: 3.3e-46, bitscore: 155.8,
                     seeds: 38, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MVVGVGGVPVGTAGDEGAAGTRRGVLRGLAAAAAGAVLTPVAVAA
                     WPEESDGKNTDVRAGDGMFEETYRGRLIRGVPIPSPEQEAGDVDWHVTVDGRPLHLMRR
                     ADGSWLSMVDHYRSYPTPLAATRAAVDELGPGQRLRDLPGHGPGHQDGGAHGVHA"
     gene            complement(5587..6105)
                     /locus_tag="A4E84_RS14205"
                     /old_locus_tag="A4E84_14205"
     CDS             complement(5587..6105)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003976098.1"
                     /locus_tag="A4E84_RS14205"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A4E84_14205"
                     /product="hypothetical protein"
                     /protein_id="WP_062926923.1"
                     /transl_table=11
                     /translation="MKSTTRGTLAAVITCVAAAAGAATATPAAAVGTVPVPVPLGGAEK
                     ALNVELPEATGQLPVPVPGSPDGPRYVEGRLIPERAVPQLPVHGGLPGADLRAPLPHIL
                     GAGFDHIGVDAPASDLRTLAPGGSVDAPLTSPHADGFGLPQAKLPQVGVLAPLVQTVPT
                     ADLGIGPGL"
     gene            complement(6242..6847)
                     /locus_tag="A4E84_RS14210"
                     /old_locus_tag="A4E84_14210"
     CDS             complement(6242..6847)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005479492.1"
                     /locus_tag="A4E84_RS14210"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A4E84_14210"
                     /product="vitamin K epoxide reductase family protein"
                     /protein_id="WP_062926924.1"
                     /transl_table=11
                     /translation="MTPPAHAGARTAGAGRAFALLLVVTGAAGLLASWIITLDKFKLLQ
                     DPDFTPGCSLNPVVSCGSVMESDQAEVFGFPNPMLGLVAYGVVICVGLSLLAGARFPRW
                     YWLAFDAGCLFGVGFVTWLQFESLYRINALCLWCCLAWAATIPLFWYVTSFAVRQGFLP
                     APGAVRRFLDEFTWVLPVLHLGIVGMLVLTRWWDFWTS"
     gene            complement(6844..7128)
                     /locus_tag="A4E84_RS14215"
                     /old_locus_tag="A4E84_14215"
     CDS             complement(6844..7128)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019325462.1"
                     /locus_tag="A4E84_RS14215"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A4E84_14215"
                     /product="hypothetical protein"
                     /protein_id="WP_062926925.1"
                     /transl_table=11
                     /translation="MKSLKAAAVVAGSLVVAGAAAPAHAAYDTDSVTPTSLNGAVNALT
                     REPIDPMPLKHQSNALDTENEDSMLSTVKQATTRLNSNGGLLGGLPLPK"
     gene            complement(7297..7923)
                     /locus_tag="A4E84_RS14220"
                     /old_locus_tag="A4E84_14220"
     CDS             complement(7297..7923)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003976096.1"
                     /locus_tag="A4E84_RS14220"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A4E84_14220"
                     /product="hypothetical protein"
                     /protein_id="WP_062926926.1"
                     /transl_table=11
                     /translation="MRLPARRIATSALCATLLLGVTGPAAVAADSASAPEHTHAASRAP
                     VPDADELLGQVESLGGLGGVLVPVADLLTAVLKADDGQLPHSQAAKLSTSVQDAIDHAT
                     ATATAPTAPGAAPQSSPLPQASTLPAPVEDDAELPEDLAAEAMTALEKSTGELLKAVTS
                     GDAAQVTPAVDAVVAGVVNAVAATLVGSRLPAPDLAGLPSLPKAP"
     gene            complement(8170..8409)
                     /gene="chpG"
                     /locus_tag="A4E84_RS14225"
                     /old_locus_tag="A4E84_14225"
     CDS             complement(8170..8409)
                     /codon_start=1
                     /gene="chpG"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007449696.1"
                     /locus_tag="A4E84_RS14225"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A4E84_14225"
                     /product="chaplin ChpG"
                     /protein_id="WP_062926927.1"
                     /transl_table=11
                     /translation="MSRIAKGLVLTSAAAAAVAGSTGIAAADAGAQAAAAHSPGVLSGN
                     VVQVPLHIPVNVCGNTVDIIGLLNPAFGNECEND"
     misc_feature    complement(8520..8529)
                     /note="TFBS match to AbrC3, Antibiotic production
                     activator, confidence: strong, score: 18.16"
                     /tool="antismash"
     misc_feature    8552..8565
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 14.73"
                     /tool="antismash"
     misc_feature    complement(8553..8566)
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 12.79"
                     /tool="antismash"
     misc_feature    complement(8554..8567)
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 12.97"
                     /tool="antismash"
     misc_feature    complement(8555..8571)
                     /note="TFBS match to HexR, Glucose-responsive regulator,
                     confidence: weak, score: 17.97"
                     /tool="antismash"
     misc_feature    8577..8592
                     /note="TFBS match to CopR, Copper-responsive regulator,
                     confidence: weak, score: 17.49"
                     /tool="antismash"
     gene            8814..9980
                     /locus_tag="A4E84_RS14230"
                     /old_locus_tag="A4E84_14230"
     CDS             8814..9980
                     /EC_number="2.4.-.-"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Glycos_transf_1"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1045: glycosyl transferase group 1"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019065680.1"
                     /locus_tag="A4E84_RS14230"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A4E84_14230"
                     /product="glycosyltransferase"
                     /protein_id="WP_062926928.1"
                     /sec_met_domain="Glycos_transf_1 (E-value: 4.3e-32,
                     bitscore: 110.0, seeds: 63, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MHLSPTDPPPRVLHVTQPVDGGVARVVTDLAGAQLAAGLHVTVAC
                     PDSPLAARLTALGADVRNWAATRAPGPSLTGEVRRLARIVEDVRPCLVHAHSAKAGLAG
                     RLAVRGRIPTVFQPHAWSFEAVGGVTAALARGWERRGARWAARVVCVSEAERRLGVRAG
                     VRGRWSVVPNGIDVERFRPADDADAVRAELLPHSRPRGPLVVCVGRLCRQKGQDVLLTA
                     WESVLRRVPRARLVLVGDGPDHGRLRALAPGSVLFAGDVDDVVPWYQAADLVVLPSRWE
                     GMALAPLEAMACGRPVVVTDVGGARESLPPGLVPHCLVPALAPAALAAAVAGLLPDPPL
                     RTALGEQGRRHVLSTHDVRRTAQAVADVYRELLAAHAVSTERRESVAS"
ORIGIN
        1 ggctcgggtt tgtcaggcgt acgaagatcg ggcatgcaaa gggcacgacc tctccgccca
       61 cacgacgggc atcgcccccc tccccgccac ctgacgacgc ggatccgcgg attcgcatgc
      121 gttcggcacg gcctacgcgt ccgcgagcgc tgagtctgcc gttcccccgg tagacctgcc
      181 gaggtcctgg aggcgccaca gcggccgcgt acgcccaagt tctgtgtgac ggagggagtt
      241 cgtgccgggg tctctcgtcg gagcggcagg cgggagcctt gcgccctcag tccgacggca
      301 tacgcctcgg gttcggtttc cggatggact ccgggccgcc cacccggcct gtccggatgc
      361 ccgtcggctc gacgcttgtc agtcgacgga ggccatgaag gccagcatcc tgcggttcat
      421 ttcctgggcg tggtcgatct gcggtccgtg cccggtaccg gtgatgatct cggcgcgggc
      481 gcccggtatc aggcggggga cgcgttccat ctgccgttcg gggtgcagca ggaggctgcg
      541 cttgccgagc accaggtaga gcggcgtgcg gatgccggac agctccgcct cggtcagggg
      601 aagcggtgcc gggcggcgga tgcggtaggc gcggatggcg gtcctgatca tcgtgcgcag
      661 ctcgggcatg accaggaccg gttgttccag ccaggccgcc agtcgcgggc gcagtttcct
      721 gggtgcgaag gtggcgaaga ggccggcgaa gatccagacg aagaagcgca gcccgacctt
      781 ctcgagtccg cccgggtcga gcaaggtgac cgaggcgagg cgttcgggcc gtcggtgtgc
      841 ctggttcagg gcgagccagc ctccgtagga ggtgccgacg aggtggacgc gttcgagccc
      901 gagcccgtcg agcgtctcgt ccagccactg tgcggcccgt tcgggctggt ggatcggctc
      961 gcgctgcacg ctgcggccgg ggtcgccggg ggtgtcgacg gcgtagacgg ggcggtcggc
     1021 gctgagggcg ggggtgttgg ggtaccacat ggcggagcag gagcccgctc cgtgtaccag
     1081 gacgaccggg gtgcgggact gggccgcttt gtcggtgggt ccgtagcgat acacgtgcgt
     1141 ggtcccgaac ttggtctcca cgtcctgctc cgcgagggcg ggtggtccca gcgcgtagac
     1201 ggcgtcgcag gcggcgaagt agcggtcgcg ccaggtgtcg ctcacatagc ggccgatgtc
     1261 ggcctgggtg cgggcagtgg tctcggacac ggccacctcc ggagggtatc gggttcgtgg
     1321 tacgaacgta tcacgatgtt gatacggctg tatcatcaaa gggccggcga ggcagcagac
     1381 gacgagcgga gaacaagccg atgcccaagc gggtggacca tgcggaacgg cgcaccgaga
     1441 tcgcggaggc gctcgtccgg gtcgccgggc gacgcgggct tcatgccgtg gggatgcgcg
     1501 atgtggcagc ggaggcgggc gtgtcactgc ggctggtgca gtactacttc gaaaccaagg
     1561 agaagttgct gctcttcgga ctggagcacc tgaccgggaa gttcggggag cggctcgccg
     1621 cccgcatccg agccgccggg gacgagccgg ggccgcgcgc gatggtcgag gcgctgctga
     1681 tgacggcgtt gccgacggat gaggagagcc gcatcttcca ccacctctac acctcgtacg
     1741 ccgttctcgc cgtgaccgac cggacgctcg cggcccagcc cttcatcaag gaccccgacg
     1801 cagccgaagg cgccctgacc gacatcctcc ggcaggcgca gaccgcggac ctgctgcagc
     1861 ccggcgtgga cccgcagttg acggcggcag gcctgctcgc gatgtccgcg gggctcggca
     1921 ccagcatcct cgtcggtcag cgcagccccg agtccgcctc cgcggtcctg gacgaccatc
     1981 tcgaccgcgt cttccgccag accggtacgg caggttgaca cacagccgtt cgcggtcctg
     2041 cttcccgcat gccccgtagc ggggggggtg agcgcgtggg acgccttcat tgaggtcacc
     2101 ggagcgaggt gagcggcatg cgggttcctc cgtgcgctcc tgcaatggcg cacgtccccc
     2161 gagctctcgc tttcagtgct ccgcaaccac gaagcggcca cgaaatcgtc gggaacggtc
     2221 aacacatctc catgatgatt tccacccgcc cctcccgtac gaatgctcgg cacgccgcgc
     2281 tcgtgcgcgg tcctgcggat gcctgtccgg gaggccgtac tgacctgcgg agtcgcgctg
     2341 ccgctggccg cggcggtctg cgggtcggtg ctggtcccgc tcagcatcgc gctcaccggc
     2401 ggctcggccg tcccggccct ggccgacggg ccgttgagcg cactggccgc cggggcgctt
     2461 gtgctcacgg ccgggaccgt cgccctcgcc ggtctcctgg ccctgtggag cgccgcggcc
     2521 atgcccgggg gcggcgaggg ccgtttcctg accacggtcc ccggcaggca cttcccgcac
     2581 ccccggcgta cgcgcgatca tggccggtga acgggaccct gggccgtgaa aggggcacga
     2641 cgtgcggctg cgctgcgctg tactggacga ctttcagcag gtggcggccg aggccgccga
     2701 ctggtcggtg gtcgcggacg acgtggaggt cgtgttcttc gaccggcaca tcgacggcga
     2761 ggacgacctg gccgccgccc tggccggctt cgacatcgtg gtgaccctgc gcgaacgcgt
     2821 cccgttcccc gggtcgctga tcacccggct gccccggctg aaactgatcg tcgcctccgg
     2881 catgcggaac tccgtcatcg actacgcggc cgccgaggaa cagggcgtca cggtgtgcgg
     2941 cacggccagt tcctccaccc cgcccgtcga actgacctgg gccctgctgc tcggcctcgc
     3001 tcgcggcatc gtcgaggaga gcaacgccct gcgctccggc ggcccctggc agagcaccgt
     3061 cggtgccgat ctgcacggcc ggcgactcgg gctgctcggc ctggggaaga tcggcggccg
     3121 ggtcgcccgg gtcgggctcg ccttcggcat gcacgtgagc gcctggagtc agaacctcac
     3181 ccgggagcgc accggcgagg tcggtgtcga acgtgccggc tcgaaggagg agctgctcgc
     3241 gggcagtgac ttcgtctccg tccacctggc cctcggcgac cgtacccggg gcctgctcgg
     3301 tccggccgaa ctcgccctcc tcaagccgac cgcctacctg atcaacacct cccgcgcggc
     3361 gatcgtcgac caggacgccc tgctcgacgc cctgcacacg ggccgtatcg ccggagccgg
     3421 cgtggacgtc ttcgacatcg agccccttcc cgcggaccac cccatgcgca cggcaccccg
     3481 cctcctggcc accccgcacc tgggctacgt ctcccgcgcc aactacgcga cctactacgg
     3541 ccacgccgtc gaggacatcc gtgcgttcct cgacggcaca ccagtgcgac ggcttccctg
     3601 acgcggcctg cccccttctg accgtcctca tggcggagcg tgcccgtcca ggacgggtga
     3661 tccggccatg atcgccgtga ccacccgggt acgggcgcgt tgaacggtga gcacggcggc
     3721 gccacctgca gccgtgctca ccgagtcaga aggagaacgt gatgtctcgc atcgcgaagg
     3781 cagccggtgc cgcactcggc acgggtgccg tggtactcag cggggccggt ctggccgtgg
     3841 ccgacgcggg tgcccagggc gcggccgtcg ggtcgccggg cgtcctctcg ggcaacgtcg
     3901 tccaggtgcc ggtccacatc ccggtcaacc tgtgcggcaa caccgtcaac gtcatcggcc
     3961 tgctcaaccc ggcgttcggc aacgagtgcg agaacaagga cgaccccaag agccaccccg
     4021 gtggctacgg gtacggccac tgatcgcacg acccaccccg tgcacgcacg caccacaccg
     4081 tgcacgcaca gagagccccg gcaccctgag cgccggggct cttcgcatgg gccgaaaggc
     4141 ggtcaccagg tcacccgtag cggtagatcc gcccgtggtc ctccagctgg tccggggtca
     4201 cgttccacgg cggcagcttc tcgtgccgcg ccacgacccg ccggtactgc gcgctgcccg
     4261 gtccgggcgg cttggccggc aggtagcggt gctcccggtg ccgccgctgc cagcgggacc
     4321 acaccaggtc gacgaaggcg tggtgcatcc agaacaccgg gtcgttgacg gaggcgccgc
     4381 cgaccatggc cccgccgacc cagcggtgca cccggttgtg gttctgccag gaggcgctgc
     4441 cctggctgct cccccatccc tccagcttgt tgcggaagcc cttgcccgtc gtcgagtccc
     4501 agggggacgc gtcgtagagc gggtccttca gggcccgttc cagctcggcc tcggtgggca
     4561 gctggatggg gtcgcgggcg cggcccaggt cccgggtgag gaactcctcg tccgtgatgc
     4621 cctccttgat ggtccagttg ccctccgcgt aggcgaacgg cccggtcatc acctggcggt
     4681 cggagcgccg gccggtgccg ccgaggaggt cggcggtcca gggggcggac gtggtcgtgc
     4741 ggtcacgcgt ccagtcccag tacggcaccg acaccgacgc gtcgacccgg cgcagcgcct
     4801 tctccaggtc cagcaggaac tgccggtgcc agggcaggaa ggagggcgcc atgtgggccg
     4861 tgcgcaggcc gtcctcgccg tcggaggtgt agtactcgat gtgcgtgcgg acgaactcgt
     4921 cgtactcgcc gcgccgtttc acctccagca tcgccttcac gaagcggcgt ttctcggtgc
     4981 gggtgagggt gctgacgtcc ttacgcgtgt acgccatggg ctcccccgtc ctggtggccc
     5041 gggccgtggc cgggcaggtc gcgcaggcgc tgccccgggc ccagttcgtc gacggcggcg
     5101 cgggtcgccg ccaggggggt ggggtaggag cggtagtggt cgaccatgct cagccagctg
     5161 ccgtccgcgc ggcgcatcag gtgcagcggc cgcccgtcca cggtgacgtg ccagtccacg
     5221 tcaccggcct cctgctccgg cgaggggatc ggcacgccgc ggatgaggcg gccccggtag
     5281 gtctcctcga acatgccgtc gccggcccgc acgtccgtgt tcttgccgtc ggactcctcg
     5341 ggccaggcgg ccacggcgac gggggtcagc acggcgccgg ccgccgcggc cgccaggccg
     5401 cgcagcacac cgcgccgggt gcccgcggcg ccctcgtccc ctgccgtgcc caccggtact
     5461 ccaccgacgc ctacgaccat gtcgtgctcc tcgttccgct cgggttgtca ctgcggtaac
     5521 agcccgaagg cccccgcggt caccaccgcg ggggccacac gagaacaccc ggtacggacc
     5581 ggcgtcctac agaccggggc cgatacccag gtccgccgtg ggcaccgtct gcaccagcgg
     5641 ggccagcacg cccacctgcg ggagcttcgc ctggggcagg ccgaagccgt ccgcgtgggg
     5701 cgaggtgagc ggggcgtcga cggacccgcc cggggcgagc gtgcgcaggt cggaggcggg
     5761 ggcgtcgaca ccgatgtggt cgaagccggc gccgaggatg tgcggcaggg gcgcccgcag
     5821 gtcggcgccg ggcagtccgc cgtggacggg cagctgcggg acggcgcgct ccgggatgag
     5881 ccggccctcg acgtagcggg gtccgtcggg cgagcccggc accggtaccg ggagctgccc
     5941 ggtggcctcg ggcagctcca cgttgagggc cttctcggcg ccgcccagcg gcacggggac
     6001 ggggaccgtg ccgaccgcgg cggcgggggt cgcggtggcg gcgccggccg cggccgccac
     6061 acacgtgatc actgcggcga gggttcctcg ggtggtcgac ttcatctggg gtacggtccc
     6121 tttcgcgtgt cgggggctca gcgtgcgtac cgagtaacga gccggggaac cgcacgagcg
     6181 cacaattccc ccgactgccg cagtaatacg acggaccgga ccgctttcgg acggccgtcg
     6241 gtcagctcgt ccagaagtcc caccagcgcg tcaggaccag catgccgacg atgccgaggt
     6301 gcagcaccgg cagcacccag gtgaactcgt cgaggaaccg gcgcaccgcc ccgggcgcgg
     6361 gcaggaaccc ctggcgcacg gcgaacgagg tcacgtacca gaacaggggg atcgtcgcgg
     6421 cccacgccag gcagcaccac aggcacagcg cgttgatccg gtacagcgac tcgaactgca
     6481 gccacgtcac gaaccccacc ccgaacaggc atccggcgtc gaaggccagc cagtaccagc
     6541 gcgggaagcg ggcaccggcc agcaggctca ggccgacgca gatgacgacg ccgtaggcga
     6601 ccagtcccag catcgggttg gggaacccga agacctcggc ctggtcgctc tccatgacgc
     6661 tgccgcagga caccaccggg ttcaggctgc agccgggcgt gaagtccggg tcctgaagca
     6721 gcttgaactt gtcgagggtg atgatccagg aggccagcag cccggccgct cccgtgacga
     6781 cgagcagcag ggcgaacgcg cggccggcgc ccgccgtgcg cgccccggcg tgcgcgggtg
     6841 gggtcacttc ggcagcggaa gaccgccgag gagcccgccg ttcgagttga gccgggtggt
     6901 cgcctgcttg acggtgctga gcatcgagtc ctcgttctcg gtgtcgagcg cgttggactg
     6961 gtgcttgagc ggcatcgggt cgatgggctc cctggtgagc gcgttgacgg cgccgttgag
     7021 gctggtgggg gtgacgctgt ccgtgtcgta ggcggcgtgc gcgggtgcgg cggcaccggc
     7081 gacgacgagg gaaccggcaa cgacggcggc ggccttcagg gacttcatcg tgaatccttt
     7141 cttcggcgac ggatgtcacc ggcggggaat tcggacgccg tgcctaacga tgcctggacg
     7201 gagcggaaac cccgtcgcca gaacctcata tgagtcgctg aacggacgcg tgccggtccg
     7261 gggggccggc acgacgaccg gtgagcgggg gcgctctcag ggggccttcg gcagggaggg
     7321 caggccggcc aggtcgggtg cgggcagccg gctgccgacc agggtcgcgg cgacggcgtt
     7381 gacgaccccg gccacgacgg cgtcgaccgc cggggtgacc tgggcggcgt caccggaggt
     7441 gacggccttg agcagctccc cggtggactt ctccagggcg gtcatggcct cggcggcgag
     7501 gtcctcgggc agctcggcgt cgtcctcgac gggggcgggc agcgtgctgg cctgcggcag
     7561 ggggctgctc tggggggcgg cgcccggcgc ggtcggggcc gtcgcggtcg cggtcgcgtg
     7621 gtcgatggcg tcctgcaccg aggtgctcag cttggccgcc tgggaatgcg gcagctggcc
     7681 gtcgtccgcc ttgagcacgg cggtcagcag gtcggcgacc gggacgagga cgccgcccag
     7741 gccgcccagg ctctcgacct ggccgagcag ttcgtccgcg tcggggacgg gggcccggga
     7801 cgccgcgtgg gtgtgctccg gggccgatgc gctgtcggcg gccacggccg cgggcccggt
     7861 gaccccgagc agcagcgtgg cgcagagcgc ggacgtcgcg attcgccgtg cgggcagacg
     7921 catgggggtt cctttcggtg gtgcgtcgga tcttgtgccc accgtgcgag cggccggaac
     7981 gctccgcaac cgagcgaacg ccccaggtca gcgccgcgtc cccgggctcg cggcgtcccc
     8041 cctggtaggc ggcgtgcgcg ttccccgtcc gccgatccgc ccgtcggccc ccgttcgggg
     8101 caacgcgaaa ccggccgctc tcccgcgtgg tcccgcagga gaacggccgg tcagggtttc
     8161 gaggcgacgt cagtcgttct cgcactcgtt gccgaacgcc gggttcagca ggccgatgat
     8221 gtcgaccgtg ttgccgcaga cgttgaccgg gatgtgcagc ggaacctgga cgacgttgcc
     8281 ggacagcacg cccggggagt gggcggcggc ggcctgcgcg ccggcgtcgg cggcggcgat
     8341 gccggtgctg cccgcgacag cggcagcggc agcggaggtc aggaccaggc ccttcgcgat
     8401 acgcgacatg gagaggtgct ccttggggtt gacacaaaca tccgggcgga tgcccggcgc
     8461 tgtgtcaacg cgtggcgttc acgcgagttg tgccgccaaa ggagtgatct cgccggaaag
     8521 gccgccttca ccgttccgac acacacgccc ggttttgaag gaaaagtacg gacagcgtct
     8581 agagtcgcaa acctctcaac gcacccctac agcaagcaaa tcgcactcag ggacgaggga
     8641 gggcaccacg gtccgcgaag ccctccggtg cggtggtgac caggtggccg tccggctcgt
     8701 ttcccagcgg gacgcggcgt gtgccgggcg tgcacttcgg cgctgtcggc acccggcagt
     8761 tcaggcgcgt tcccgccgtc ccgagcgcca tccgtgagcg aggaataccg cgcatgcacc
     8821 tgtcaccgac cgaccctccg ccacgggtcc tccacgtcac gcagcccgtg gacggcgggg
     8881 tcgcccgcgt cgtgacggac ctggccgggg cgcagctggc cgcgggcctg cacgtcacgg
     8941 tcgcctgccc cgacagcccc ctcgcggccc ggctcacggc gctcggcgcc gacgtacgga
     9001 actgggcggc gacgcgtgcg ccgggaccgt cgctcacggg cgaggtacgg cggctcgccc
     9061 ggatcgtcga ggacgtacgg ccctgtctgg tgcacgcgca cagcgcgaag gccgggctgg
     9121 ccgggcggct cgccgtgcgg gggcgcatcc cgacggtctt ccagccgcac gcctggtcgt
     9181 tcgaggcggt gggcggcgtc accgcggccc tcgcccgcgg ctgggagcgg cggggggccc
     9241 gctgggcggc gcgagtggtg tgcgtcagcg aggcggaacg ccggctgggg gtgcgggccg
     9301 gggtccgcgg gcggtggagc gtcgtcccca acgggatcga cgtcgagcgc ttccgtccgg
     9361 ccgacgacgc cgacgccgtg cgggcggagc tgctgccgca cagccgtccg cgggggccgc
     9421 tcgtggtgtg cgtggggcgg ttgtgccggc agaaggggca ggacgtcctg ctgacggcgt
     9481 gggagtccgt actgcggcgg gtgccccggg cgcgactggt gctggtcggc gacgggccgg
     9541 accacggccg gctgcgggcc ctggcccccg gctcggtgct gttcgcgggg gacgtcgacg
     9601 atgtcgtgcc ctggtaccag gcggcggacc tggtcgtcct gccgtcgcgg tgggagggca
     9661 tggcgctggc cccgctggag gccatggcgt gcgggcggcc ggtggtggtg acggacgtcg
     9721 gcggggcccg ggagagcctg ccgccgggcc tcgtaccgca ctgcctggtc cccgccctgg
     9781 ccccggcggc actggccgcg gccgtcgccg ggctgctgcc ggacccgccg ctgcgcaccg
     9841 cgctcggcga gcaggggcgc cggcacgtcc tgtccacgca cgacgtgcgc cgcacggctc
     9901 aggcggtcgc ggacgtgtac cgcgaactcc tggccgcgca cgccgtgtcc accgagcgca
     9961 gggagtctgt cgcctcgtga ccgcggaaag caccgttccc acccccgccg ggcagccgcg
    10021 ggacccccgg ttcccgtccg tgtcggtcct gccgccgcgc ggcgccgccg cgggtttccg
    10081 cttccccggc cggatgccgc cggcccggcc cgtgtcgccg ctgccgctgc tcgcagcgga
    10141 cggcacggcg gccgtgctgg gcgggctggc gctgtccggg acccaacgcc acccgctgct
    10201 gctggcggtg ctggtggcgg cctcgctgct gctgcgtccg ccccggcccg gccgggtgcc
    10261 ggccgtgctg gacgaactgc ccgccgtggg cggccggatc gcggtggcct ggctggcact
    10321 cggggcgttc gtggcggcgt acaccccgca gcacctgctc tccgcctcga ccctgctcgc
    10381 cggcttcctg ctgcagaccg cggcgggctg cgccgggcgc ggcgcggtgc acgcgtgccg
    10441 gcgcgccgaa ctgctgcacc ggccgcaggc cgcgctcgtg
//
