LOCUS       NZ_CP015098            10399 bp    DNA     linear   CON 03-MAR-2024
DEFINITION  Streptomyces qaidamensis strain S10 chromosome, complete genome.
ACCESSION   NZ_CP015098
VERSION     NZ_CP015098.1
KEYWORDS    RefSeq.
SOURCE      Streptomyces qaidamensis
  ORGANISM  Streptomyces qaidamensis
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces; Streptomyces aurantiacus group.
REFERENCE   1  (bases 1 to 9083372)
  AUTHORS   Zhang,B.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-APR-2016) State Key Laboratory of Cryospheric
            Sciences, Chinese Academy of Sciences, Donggang West Road 320,
            Lanzhou, Gansu 730000, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SMRT Analysis v. 2.3.0
            Genome Coverage                   :: 278x
            Sequencing Technology :: Illumina HiSeq 4000; PacBio RSII
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 03/03/2024 10:55:15
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,226
            CDSs (total)                      :: 8,137
            Genes (coding)                    :: 7,871
            CDSs (with protein)               :: 7,871
            Genes (RNA)                       :: 89
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 266
            CDSs (without protein)            :: 266
            Pseudo Genes (ambiguous residues) :: 0 of 266
            Pseudo Genes (frameshifted)       :: 119 of 266
            Pseudo Genes (incomplete)         :: 213 of 266
            Pseudo Genes (internal stop)      :: 25 of 266
            Pseudo Genes (multiple problems)  :: 80 of 266
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 16:26:55
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 2149542
            Orig. end                         :: 2159941
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP015098.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10399
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="ectoine"
                     /region_number="7"
                     /rules="ectoine_synt"
                     /tool="antismash"
     cand_cluster    1..10399
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="ectoine_synt"
                     /kind="single"
                     /product="ectoine"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10399
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5399](+)"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5399
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
     gene            1024..2124
                     /locus_tag="A4E84_RS09395"
                     /old_locus_tag="A4E84_09395"
     CDS             1024..2124
                     /NRPS_PKS="Domain: Aminotran_5 (73-339). E-value: 2.6e-29.
                     Score: 94.2. Matches aSDomain:
                     nrpspksdomains_A4E84_RS09395_Aminotran_5.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_5"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1139: aminotransferase class V"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010037833.1"
                     /locus_tag="A4E84_RS09395"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A4E84_09395"
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="WP_062926101.1"
                     /sec_met_domain="Aminotran_5 (E-value: 1e-22, bitscore:
                     79.5, seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTHPFLDLAPLGADRFAAIEDRVARLLSTEQDVVIMQGEALLPLE
                     GAIRAAAGPGTTALNVITGPYGQTFGDWLRDCGATVIDLAVPFHTAVTAEEIRQAFAEH
                     PQIDFVSLVHAEAATGNTNPVAEIGEIVRAHGALFYLDAVASIGAEPVLPDAWGVDLCV
                     IGAQKAMGGPAGVSAVSVSERAWARMAANPKAPRRSYLSLLDWKERWIDGGRRALLHAP
                     AQLEMLALEACVERIEAAGAEAVMARHASAAAATRAGAVALGGGLEPYVYEARDAAPVA
                     TTLRAPSGLVASELVRRALEADPAVPLAAGGGALAKEMIRVNHYGADATPGAVRASLAA
                     LGVALSEQGLSVDVEAALRAVEGAWR"
     aSDomain        1243..2040
                     /aSDomain="Aminotran_5"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_A4E84_RS09395_Aminotran_5.1"
                     /evalue="2.60E-29"
                     /label="A4E84_RS09395_Aminotran_5.1"
                     /locus_tag="A4E84_RS09395"
                     /protein_end="339"
                     /protein_start="73"
                     /score="94.2"
                     /tool="antismash"
                     /translation="LRDCGATVIDLAVPFHTAVTAEEIRQAFAEHPQIDFVSLVHAEAA
                     TGNTNPVAEIGEIVRAHGALFYLDAVASIGAEPVLPDAWGVDLCVIGAQKAMGGPAGVS
                     AVSVSERAWARMAANPKAPRRSYLSLLDWKERWIDGGRRALLHAPAQLEMLALEACVER
                     IEAAGAEAVMARHASAAAATRAGAVALGGGLEPYVYEARDAAPVATTLRAPSGLVASEL
                     VRRALEADPAVPLAAGGGALAKEMIRVNHYGADATPGAVRASLA"
     gene            2242..2778
                     /locus_tag="A4E84_RS09400"
                     /old_locus_tag="A4E84_09400"
     CDS             2242..2778
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004002247.1"
                     /locus_tag="A4E84_RS09400"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A4E84_09400"
                     /product="DinB family protein"
                     /protein_id="WP_062926102.1"
                     /transl_table=11
                     /translation="MTDTRAEASPTLPDGRPVPHLTGDERAMLESWLDFHRATLELKCA
                     GLDDAQVRVAAAEPSSLTLLGLVQHLAEVERNWFQRVAGGVAVAPVFEDATGYSLDPAR
                     GLDAALGIWRREIARGRELCAGLPLDHIGRVAEGPVPGMEVSLRWVLIHMIEEYARHNG
                     HADLLRERIDGVTGA"
     misc_feature    complement(2948..2963)
                     /note="TFBS match to CRP, Cyclic AMP receptor protein,
                     confidence: medium, score: 16.52"
                     /tool="antismash"
     misc_feature    complement(2984..2998)
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 16.83"
                     /tool="antismash"
     misc_feature    3082..3094
                     /note="TFBS match to LuxO, Repressor of luminescence,
                     confidence: weak, score: 17.04"
                     /tool="antismash"
     gene            3119..3625
                     /gene="ectA"
                     /locus_tag="A4E84_RS09405"
                     /old_locus_tag="A4E84_09405"
     CDS             3119..3625
                     /EC_number="2.3.1.178"
                     /GO_function="GO:0033816 - diaminobutyrate
                     acetyltransferase activity [Evidence IEA]"
                     /GO_process="GO:0019491 - ectoine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="ectA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003989362.1"
                     /locus_tag="A4E84_RS09405"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A4E84_09405"
                     /product="diaminobutyrate acetyltransferase"
                     /protein_id="WP_062926103.1"
                     /transl_table=11
                     /translation="MTAVQADPQIDRPTVADGAALWRMAKDSKVLDLNSSYSYLLWCRD
                     FAATSAVARDAQGEPMGFITGYVRPDSPHTLLVWQVAVDEAHRGRGLAAALLDGLVART
                     TTELEVTTVETTITPGNTASERLFTSFAERHGARLEREVLFDTGLFPDGPHDAEVLYRI
                     GPLPH"
     gene            3685..4956
                     /gene="ectB"
                     /locus_tag="A4E84_RS09410"
                     /old_locus_tag="A4E84_09410"
     CDS             3685..4956
                     /EC_number="2.6.1.76"
                     /GO_function="GO:0045303 - diaminobutyrate-2-oxoglutarate
                     transaminase activity [Evidence IEA]"
                     /NRPS_PKS="Domain: Aminotran_3 (29-366). E-value: 7.3e-74.
                     Score: 240.7. Matches aSDomain:
                     nrpspksdomains_A4E84_RS09410_Aminotran_3.1"
                     /codon_start=1
                     /gene="ectB"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_3"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1013: aminotransferase class-III"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007448159.1"
                     /locus_tag="A4E84_RS09410"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A4E84_09410"
                     /product="diaminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="WP_062926104.1"
                     /sec_met_domain="Aminotran_3 (E-value: 2.3e-78, bitscore:
                     262.5, seeds: 13, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTITQPDLSVFETVESEVRSYCRGWPTVFDRAVGSRMYDEDGHEY
                     LDFFAGAGSLNYGHNNPVLKRALIDYLERDGVTHGLDMSTTAKRTFLEAFQNLLLRPRD
                     LPYKVMFPGPTGTNAVEAALKLARKVKGRESIVSFTNAFHGMSLGSLAVTGNAFKRAGA
                     GIPLVHGTPMPFDNYFDGTVEDFLWFERLLEDQGSGLNKPAAVIVETVQGEGGINVARA
                     EWLRALSELCERQDMLLIVDDIQMGCGRTGAFFSFEEAGITPDIVTVSKSISGYGLPMS
                     LCLFKPELDVWEPGEHNGTFRGNNPAFVTATAALETYWADGSAMEKQTRKRGEQVEQAL
                     ISITEENLADVKEYRGRGLVWGMEFHDKARAGKVAKRAFELGLLIETSGPESEVVKLLP
                     ALTITPEELDEGLSMLARAVRETA"
     aSDomain        3772..4782
                     /aSDomain="Aminotran_3"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_A4E84_RS09410_Aminotran_3.1"
                     /evalue="7.30E-74"
                     /label="A4E84_RS09410_Aminotran_3.1"
                     /locus_tag="A4E84_RS09410"
                     /protein_end="366"
                     /protein_start="29"
                     /score="240.7"
                     /tool="antismash"
                     /translation="DRAVGSRMYDEDGHEYLDFFAGAGSLNYGHNNPVLKRALIDYLER
                     DGVTHGLDMSTTAKRTFLEAFQNLLLRPRDLPYKVMFPGPTGTNAVEAALKLARKVKGR
                     ESIVSFTNAFHGMSLGSLAVTGNAFKRAGAGIPLVHGTPMPFDNYFDGTVEDFLWFERL
                     LEDQGSGLNKPAAVIVETVQGEGGINVARAEWLRALSELCERQDMLLIVDDIQMGCGRT
                     GAFFSFEEAGITPDIVTVSKSISGYGLPMSLCLFKPELDVWEPGEHNGTFRGNNPAFVT
                     ATAALETYWADGSAMEKQTRKRGEQVEQALISITEENLADVKEYRGRGLVWGMEFH"
     gene            5001..5399
                     /locus_tag="A4E84_RS09415"
                     /old_locus_tag="A4E84_09415"
     CDS             5001..5399
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     ectoine: ectoine_synt"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009337952.1"
                     /locus_tag="A4E84_RS09415"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A4E84_09415"
                     /product="ectoine synthase"
                     /protein_id="WP_033310882.1"
                     /sec_met_domain="ectoine_synt (E-value: 2.2e-54, bitscore:
                     181.6, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIVRSFKEIEGTDRHVKAASGTWESKRIVLAKEKVGFSLHETILY
                     AGTETSMWYANHIEAVVCVEGEAELTDHETGQKHTITPGTMYLLDGHERHTLRVKQDFR
                     CLCVFNPPVTGREDHDENGVYPLLTEEV"
     gene            5407..6315
                     /gene="thpD"
                     /locus_tag="A4E84_RS09420"
                     /old_locus_tag="A4E84_09420"
     CDS             5407..6315
                     /EC_number="1.14.11.55"
                     /codon_start=1
                     /gene="thpD"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PhyH"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003989365.1"
                     /locus_tag="A4E84_RS09420"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A4E84_09420"
                     /product="ectoine hydroxylase"
                     /protein_id="WP_062926105.1"
                     /sec_met_domain="PhyH (E-value: 1.9e-38, bitscore: 131.8,
                     seeds: 39, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTATTDTTTGIPDLYPSRGAAEALTPRQDPVVWGAPDAPGPVTPA
                     DLLSFERDGFLAIDELISEDEVAVYRNELNRLVNDPDMRADERSIVEPKSKEIRTVFEV
                     HKISEVFARLVRDERVVGRARQILGSDVYVHQSRINVKPGFGASGFYWHSDFETWHAED
                     GLPRMRTVSVSIALTENYDTNGGLMIMPGSHKTFLGCAGETPKDNYKKSLQMQDAGTPS
                     DEALTQLATQHGIKLFTGKAGSATWFDCNAMHGSGDNITPFPRSNVFIVFNSVENTAVE
                     PFAAPVRRPEFIGARDFTPVR"
     gene            complement(6568..7620)
                     /locus_tag="A4E84_RS09425"
                     /old_locus_tag="A4E84_09425"
     CDS             complement(6568..7620)
                     /NRPS_PKS="Domain: Aminotran_5 (17-344). E-value: 4.5e-24.
                     Score: 77.0. Matches aSDomain:
                     nrpspksdomains_A4E84_RS09425_Aminotran_5.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_5"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1139: aminotransferase class V"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010037842.1"
                     /locus_tag="A4E84_RS09425"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A4E84_09425"
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="WP_062926106.1"
                     /sec_met_domain="Aminotran_5 (E-value: 3.1e-18, bitscore:
                     64.8, seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="METFENLVRAEFTPKHTYLNTASNGLLPARTVSAVQQAVRMRAEG
                     TPLGPLYEDVEAARASFARLAGVPAERVATGASVAEYGALIAASLPAGAEVLTAESDFA
                     SLVNPFHVRGDLKVRAVPLERLAESVRPGTALVAVSAAQSADGRIADLPAVREAARAHG
                     ARTYVDASQSAGWLPMEADADDFLAAVGFKWLMGPHGASFFVAPQDFGGLTPLLAGWVA
                     GEVPWDSCYGPVAELAHSARRFDVSPALFTYAGLRASLELLEEIGVDTVHAHDVGLADR
                     FRAGLAGLGQEPVPAPGSAIVSVPGLGHRQGELSRAGIEVSNRAGNLRAAFHLYNTPAD
                     VDRLLEVLAA"
     aSDomain        complement(6589..7569)
                     /aSDomain="Aminotran_5"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_A4E84_RS09425_Aminotran_5.1"
                     /evalue="4.50E-24"
                     /label="A4E84_RS09425_Aminotran_5.1"
                     /locus_tag="A4E84_RS09425"
                     /protein_end="344"
                     /protein_start="17"
                     /score="77.0"
                     /tool="antismash"
                     /translation="YLNTASNGLLPARTVSAVQQAVRMRAEGTPLGPLYEDVEAARASF
                     ARLAGVPAERVATGASVAEYGALIAASLPAGAEVLTAESDFASLVNPFHVRGDLKVRAV
                     PLERLAESVRPGTALVAVSAAQSADGRIADLPAVREAARAHGARTYVDASQSAGWLPME
                     ADADDFLAAVGFKWLMGPHGASFFVAPQDFGGLTPLLAGWVAGEVPWDSCYGPVAELAH
                     SARRFDVSPALFTYAGLRASLELLEEIGVDTVHAHDVGLADRFRAGLAGLGQEPVPAPG
                     SAIVSVPGLGHRQGELSRAGIEVSNRAGNLRAAFHLYNTPADVDRL"
     gene            complement(7717..8433)
                     /locus_tag="A4E84_RS09430"
                     /old_locus_tag="A4E84_09430"
     CDS             complement(7717..8433)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003989367.1"
                     /locus_tag="A4E84_RS09430"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A4E84_09430"
                     /product="DsbA family oxidoreductase"
                     /protein_id="WP_062926107.1"
                     /transl_table=11
                     /translation="MRVEIWSDIACPWCYVGKARFEKALRDFPYREDVEVVHRSFELDP
                     GRAKDDIQPVITMLTKKYGMSAAQAEAGEDNLGAQAAAEGLDYRTRGRDHGSTFDMHRL
                     LHFAEEQGRQEQLLDLLYRANFAEERSVFNDDERLVELASAAGLDSEAVRAVLADPDAY
                     ADEVRADEREAAQLGANGVPFFVLDRKYGVSGAQPTEVFTRALTQAYGEAPPLKIVDSG
                     ESADACGPDGCAVPQH"
     misc_feature    complement(8483..8496)
                     /note="TFBS match to ArgR, Regulator of arginine
                     biosynthesis genes, confidence: weak, score: 17.0"
                     /tool="antismash"
     gene            complement(8508..9002)
                     /locus_tag="A4E84_RS09435"
                     /old_locus_tag="A4E84_09435"
     CDS             complement(8508..9002)
                     /GO_function="GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003989368.1"
                     /locus_tag="A4E84_RS09435"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A4E84_09435"
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="WP_174569413.1"
                     /transl_table=11
                     /translation="MINDRLTPDRVIRTALPAEVADVVAVHARARATYYPDGLPQDGTD
                     WSAIWRSALTRPDGRVLCVVEGGRIVGLASFRVPEDTPADTVALFQFHVDPDHWRHGVG
                     SALHAACVEEWQADGRRTAVLDVHVDNVRAQGFYRRQGWVPEPAGPGDHHLRMRFVLPG
                     E"
     gene            complement(9048..9656)
                     /locus_tag="A4E84_RS09440"
                     /old_locus_tag="A4E84_09440"
     CDS             complement(9048..9656)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016436202.1"
                     /locus_tag="A4E84_RS09440"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="A4E84_09440"
                     /product="DUF1349 domain-containing protein"
                     /protein_id="WP_062926108.1"
                     /transl_table=11
                     /translation="MDLEFSELPFGLRTYGPDGHWSYEDGVLTGWAGARQDRFVPPTGE
                     GLDPASDAPRLLGAPEGDFQLIAKVTVGFGAAFDAGVLYVHVGERAWAKLCLEYSPDVP
                     TVCTVVTRGHSDDANSFTVEGSSVWLRVSRTGRAFAFHASRDGERWTFVRLFTLGEEKE
                     TEAALVGFMTQSPMGEGCVVTFDHIEFRPAWPDDLRDGS"
     misc_feature    complement(9632..9646)
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 19.08"
                     /tool="antismash"
ORIGIN
        1 cgacctcggc cgcggccagg ccgtgcgcga ggccaccgcc ggcgtcggtg ccgacgaaga
       61 ccgggatccc ggcgtcgtag gcgccccgga cggtgtcgta gcggcgttcg tggagcctgc
      121 gcatatgggc cgaccagcgg gggaacttgc tctccccgcc gtccgcgagc tgcgggaacg
      181 tcgcgatgtt gaccagggtc gggacgatgg cgacgccgcg ttcggcgaac agcgggacga
      241 ggtcctcggt caggcccgtc gcgtgttcga tgcagtcgat gcccgcctcg acgaggtcct
      301 tgagggagtc ctccgcgaag cagtgcgcgg tcacccgcgc gccgaggcgg tgggcctcgg
      361 cgatcgccgc ctcgacggcc ccgcggggcc agcaggccga caggtcgccg aggtcgcggt
      421 cgatccagtc gccgaccagc ttgacccagc cgtcgccgcg ccgggcctcc tgggcgacat
      481 aggcgaccag gtcctccggc tcgatctccc aggcgtagtt gcggatgtag cggcgggtgc
      541 gggcgatgtg ccgccctgcg cggatgatct tcggcaggtc ctcgcggtcg tcgatccagc
      601 gggggtcgga gggcgagccg gcgtcacgga tgaggagagt gcccgcgtcc cggtcggtca
      661 gggcctgctt ctccgcgacg tcctggggga ccggtccgtg ccggtcgagt ccgacgtggc
      721 agtgcgcgtc gacgaggccg ggcagggccc agccgacgac cgtgcggacg tcgcgggcgc
      781 cggcgggacg gtcgtaggag atgcggccgt cgaccatcca cagctcgtcc cggacctcct
      841 cgggcccgac gagcacccgc cccttcacgt gcagcaccgt gcgatcgctc atgcaccgca
      901 ccttagtgag ccggccgccc ggcctcgaaa ggggccggtg tccgggggac gggccgcgcg
      961 ggggcgcgag ggccgccggt taccctcgat gcgatcgaac ctgtgagtga agagagcccc
     1021 accgtgacgc atccgtttct ggacctggcc ccgctcggcg cggaccgctt cgccgcgatc
     1081 gaggaccgtg tggcgcggct gctgagcacc gagcaggacg tcgtgatcat gcagggcgag
     1141 gcgttgctgc cgctggaggg cgcgatccgc gccgcggccg gtcccggcac gacggcgctg
     1201 aacgtcatca cggggcccta cgggcagacc ttcggcgact ggctgcggga ctgcggcgcc
     1261 acggtgatcg acctggcggt gcccttccac acggcggtga cggccgagga gatcaggcag
     1321 gccttcgccg agcacccgca gatcgacttc gtgtcgctgg tgcacgcgga ggcggcgacc
     1381 ggcaacacca acccggtcgc ggagatcggc gagatcgtga gggcccacgg ggcgctgttc
     1441 tacctggacg ccgtggcgtc gatcggggcc gagccggtgc tgccggacgc gtggggcgtg
     1501 gatctgtgcg tgatcggcgc gcagaaggcg atgggcggtc cggccggggt gtcggcggtg
     1561 tccgtgagcg agcgggcctg ggcgcggatg gccgccaacc cgaaggctcc gcgccggtcg
     1621 tacctgtcgc tcctcgactg gaaggagcgg tggatcgacg gcggccgcag ggcgctgctg
     1681 cacgcgcccg cgcaactgga gatgctggcg ctggaggcgt gcgtggagcg gatcgaggca
     1741 gcgggagcgg aggccgtgat ggcccggcac gcgtccgccg cggcggcgac ccgggccggt
     1801 gcggtcgcgc tcggtggcgg tctggagccg tatgtgtacg aggcccggga cgcggcgccc
     1861 gtcgccacga cgctgcgggc gccctccggg ctggtggcgt cggagctggt ccggcgggcg
     1921 ctggaggcgg atccggccgt gccgttggcc gcgggcgggg gtgcgctggc caaggagatg
     1981 atccgggtca accactacgg cgcggacgcg acgccgggcg cggtgcgggc gagtctggcg
     2041 gcgctgggcg tggcgctctc cgagcagggg ctgtcggtgg atgtggaggc ggcgctgcgg
     2101 gccgtcgagg gggcgtggag gtagtcgtct ggccggggtt cgttcctcgc ccccgccggc
     2161 gccgggccgg acgggctgaa agacaccggt cggcgccgac ggccggcgag cgatgtcagt
     2221 gacgcatgcc atgctcccgg catgaccgac acacgcgccg aagcatcccc gacccttccc
     2281 gacggccgcc ccgtcccgca tctgaccggt gacgagcgcg ccatgctgga gagctggctg
     2341 gacttccacc gggccacgct ggagctgaag tgcgccggtc tggacgacgc gcaggtgcgg
     2401 gtcgctgcgg ccgagccgtc gtcgctgacc ctgctcgggc tggtgcagca cctcgccgag
     2461 gtcgagcgga actggttcca gcgggtggcc ggcggggtgg ccgtggcgcc cgtgttcgag
     2521 gacgcgactg gctactcgct cgatccggcg cgcgggctcg acgcggcgct cggaatctgg
     2581 cggcgggaga tcgcgcgggg gcgggagctg tgcgccgggc tgccgctgga ccacatcggg
     2641 cgggtcgccg aggggccggt gcccgggatg gaggtcagtc tgcgctgggt gctcatccac
     2701 atgatcgagg agtacgcgcg gcacaacggt cacgccgatc tcctgcggga acgtatcgac
     2761 ggagtgaccg gggcctgaat tccccgtgct ttccgtgata tctcacatgt aagctggcac
     2821 acaattaatg aattctttca tgaacggctt cccgtattct tctgcccgct tttcccgttc
     2881 ctaaacgcgc agattttgag gacgctcgga ggggaaattc cgacaccccc cgcaaggtcc
     2941 acgcagctgt gacgcgttac acactgtgac cactttgccc gggaattacc ggacaatacc
     3001 gggcatcctg ctgccttgat acgtgaaccc tcgcgctcgc gcgataacac agacggggcc
     3061 catatgtaac cctcatcggc gatgcaattt tgaatttcgc tgggtaagtt caattcgcat
     3121 gactgccgta caagcagatc cgcaaatcga ccgccccacg gtggctgacg gagccgcact
     3181 ctggcggatg gcgaaggact cgaaggttct cgacctgaac tcgtcctaca gctatctgct
     3241 gtggtgtcgg gacttcgccg ccacctcggc cgtcgcccgc gacgcgcagg gcgagccgat
     3301 gggtttcatc accgggtacg tgcggccgga cagcccgcac accctgctgg tctggcaggt
     3361 ggcggtggac gaggcccacc gcgggcgcgg actggccgcc gcgctgctcg acggcctggt
     3421 cgcacggacc acgaccgagc tggaggtgac gacggtggag accaccatca ccccgggcaa
     3481 caccgcctcg gagcgcctgt tcacgtcgtt cgccgagcgt cacggcgccc ggctggagcg
     3541 cgaggtgctg ttcgacacgg gcctgttccc cgacgggccg cacgacgcag aggtgctgta
     3601 ccgcatcggc ccgctgcccc actgagactt cccgccccac cccactcccg cagacttccc
     3661 ccacgtaccg aggagcgatt cgtcgtgacc atcacccagc ccgacctgag cgtcttcgag
     3721 accgtcgagt ccgaggtgcg cagttactgc cgcggctggc ccaccgtctt cgaccgtgcg
     3781 gtgggcagcc gcatgtacga cgaggacggc catgagtacc tcgacttctt cgccggagcc
     3841 gggtcactga actacgggca caacaacccg gtcctgaaac gcgcgctgat cgactacctg
     3901 gagcgcgacg gcgtcacgca cgggctcgac atgtcgacca ccgccaagcg cacgttcctt
     3961 gaggccttcc agaacctgct gctgcgcccg cgcgacctgc cgtacaaggt catgttcccc
     4021 ggcccgaccg gcaccaacgc cgtcgaggcc gccctgaagc tggcgcggaa ggtgaagggg
     4081 cgcgagtcga tcgtgtcctt caccaacgcc ttccacggca tgtcgctggg ctcgctggcc
     4141 gtgaccggca acgccttcaa gcgcgccggc gccggtatcc cgctggtcca cggcaccccg
     4201 atgccgttcg acaactactt cgacggcacg gtcgaggact tcctgtggtt cgagcggctc
     4261 ctggaggacc agggctcggg cctcaacaag cccgccgccg tgatcgtcga gaccgtgcag
     4321 ggcgagggcg gcatcaacgt ggcccgggcc gagtggctgc gcgccctgtc cgagctgtgc
     4381 gagcggcagg acatgctgct gatcgtcgac gacatccaga tgggctgcgg ccgtaccggc
     4441 gcgttcttct ccttcgagga ggcgggcatc acgccggaca tcgtcaccgt ctccaagtcg
     4501 atcagcggct acggcctgcc catgtcgctg tgcctgttca agccggagct cgacgtgtgg
     4561 gagcccggcg agcacaacgg caccttccgc ggcaacaacc ccgcgttcgt cacggccacc
     4621 gcggccctgg agacgtactg ggccgacggt tccgccatgg agaagcagac ccgcaagcgc
     4681 ggcgagcagg tcgagcaggc gctgatctcc atcaccgagg agaacctggc cgacgtgaag
     4741 gagtaccggg gccgtggcct ggtgtggggc atggagttcc acgacaaggc ccgcgccggc
     4801 aaggtcgcca agcgcgcctt cgagctcgga ctgctcatcg agacgtccgg cccggagagc
     4861 gaggtcgtga agctgctccc ggcgctcacc atcacgcccg aggagctgga cgagggcctg
     4921 agcatgctgg cccgcgccgt ccgagagacc gcctgacaca gcaccgagag caccacccat
     4981 ctaggaggca tcgcagcacc gtgattgtcc gttcgttcaa ggagatcgaa ggtaccgacc
     5041 ggcacgtgaa ggcggcgtcc ggcacgtggg agagcaaacg catcgtcctc gccaaggaga
     5101 aggtcggctt ctccctgcac gagacgatcc tgtacgcggg tacggagacg tcgatgtggt
     5161 acgcgaacca catcgaggcc gtcgtctgcg tggagggcga ggccgagctg accgaccacg
     5221 agaccgggca gaagcacacc atcacgcccg gcacgatgta cctcctggac gggcacgaga
     5281 ggcacacgct gcgggtcaag caggacttcc gctgcctctg cgtgttcaac ccgcccgtga
     5341 ccggacggga ggaccacgac gagaacggcg tctacccgct gctcaccgag gaggtgtgac
     5401 gtttccatga ccgccactac cgacaccacc accggtatcc ccgacctcta ccccagtcgc
     5461 ggtgccgccg aggcgctgac cccgcgccag gacccggttg tctggggcgc acccgacgcc
     5521 ccgggcccgg tcaccccggc cgacctgctg tccttcgagc gcgacggctt cctggccatc
     5581 gacgagctca tcagcgagga cgaggtcgcg gtctaccgca acgagctgaa ccggctggtg
     5641 aacgacccgg acatgcgggc cgacgagcgg tcgatcgtcg agccgaagtc caaggagatc
     5701 cgcacggtct tcgaggtgca caagatcagc gaggtgttcg cccggctggt ccgcgacgag
     5761 cgggtcgtcg gacgggcgcg gcagatcctc ggctcggacg tgtacgtcca ccagtcgcgg
     5821 atcaacgtca agccggggtt cggcgccagt ggcttctact ggcactcgga cttcgagacg
     5881 tggcacgccg aggacggtct gccgcggatg cgcacggtgt cggtctcgat cgcgctgacc
     5941 gagaactacg acaccaacgg cggtctcatg atcatgccgg ggtcgcacaa gacgttcctc
     6001 gggtgcgcgg gggaaactcc gaaggacaac tacaagaagt cgctgcagat gcaggacgcg
     6061 ggcacgccgt ccgacgaggc gctgacccag ctggccacgc agcacggcat caagttgttc
     6121 acgggcaagg ccggttcggc gacgtggttc gactgcaacg ccatgcacgg ttccggcgac
     6181 aacatcacgc cgttcccgcg cagcaacgtg ttcatcgtgt tcaacagcgt ggagaacacg
     6241 gcggtggaac cgttcgcggc accggtgcgg cggccggagt tcatcggcgc gcgggacttc
     6301 acgccggtgc ggtgaccgcc cctcacccgg ccgggggtcc gggggtcgcc cccggagaaa
     6361 cacagcatcg gcgcgcggga cttcacgccc gtgcggtgac cgccctcacc cggccggggg
     6421 tccgggggtc gcccccggag aaacacagca tcggcgcgcg ggacttcacg cccgtgcggt
     6481 gaccgccggt cagcggtgag acgaccgtgt gacagagctg ccgggtgtgg cctcctcgtg
     6541 tgggacggga ggccccaccc ggttctgtca tgccgccagg acctccagca gccggtccac
     6601 gtccgcgggc gtgttgtaga ggtggaacgc ggcccgcagg ttcccggccc ggttcgagac
     6661 ctcgatgccc gcccggctca gttcgccctg ccggtggccg agccccggca ccgacacgat
     6721 cgccgacccc ggtgcgggca cgggctcctg gcccagcccg gcgagcccgg cgcggaagcg
     6781 gtccgcgagg ccgacgtcat gggcgtgcac ggtgtcgacg ccgatctcct ccagcagttc
     6841 cagtgaggcg cgcagccccg cgtaggtgaa gagggccggg ctgacgtcga accgccgcgc
     6901 cgagtgggcg agttccgcca cggggccgta gcagctgtcc caggggacct cgccggcgac
     6961 ccagccggcg agcagcggtg tgagccctcc gaagtcctgc ggggcgacga agaacgaggc
     7021 cccgtgcggg cccatgagcc acttgaagcc gacggcggcg aggaagtcgt ccgcgtcggc
     7081 ctccatcggc agccagccgg ccgactggga ggcgtcgacg taggtgcggg cgccgtgcgc
     7141 ccgggccgcc tcgcgcaccg cgggcaggtc ggcgatgcgg ccgtcggcgg actgcgcggc
     7201 gctgaccgcg acgagcgcgg tgccggggcg gacggactcg gcgagccgct ccagggggac
     7261 ggcccgcacc ttgaggtcgc cgcgcacatg gaacgggttc accagggacg cgaagtcgct
     7321 ctccgccgtg aggacttcgg caccggcggg gagcgaggcg gcgatcagcg caccgtactc
     7381 ggcgaccgag gccccggtcg cgacgcgctc ggccgggacg ccggccagcc gggcgaagga
     7441 cgcgcgggcg gcctccacgt cctcgtagag gggacccagg ggcgtgccct cggcgcgcat
     7501 tcgcaccgcc tgctgcacgg cgctgacggt acgggcgggc agcaggccgt tgctcgcggt
     7561 gttgaggtag gtgtgcttcg gggtgaactc ggcacggacg aggttctcga aggtctccat
     7621 gggaccactc tgcgtccccc ggatctcccc gtccattgcc gatttctgcg tgatgccgct
     7681 aaggagcgct tatggatgcg ctccgagctg ggggtttcag tgctggggca cggcgcagcc
     7741 gtccgggccg caggcgtccg cgctctcgcc gctgtcgacg atcttcaggg gcggggcctc
     7801 gccgtacgcc tgggtgaggg cccgggtgaa gacctcggtg ggctgggcgc cggagacgcc
     7861 gtacttgcgg tcgagcacga agaacggcac accgttcgcg ccgagctgtg cggcctcgcg
     7921 ctcgtcggcc cggacctcgt cggcgtacgc gtccgggtcg gcgagcaccg cgcgtacggc
     7981 ctcgctgtcg agaccggcgg ccgaggcgag ctccaccagc cgctcgtcgt cgttgaagac
     8041 ggaccgctcc tcggcgaagt tggcccggta gagcaggtcg agcagctgct cctggcggcc
     8101 ctgctcctcg gcgaagtgca gcaggcggtg catgtcgaaa gtgctgccgt ggtcgcggcc
     8161 ccgggtgcgg tagtcgaggc cttccgccgc agcctgggcg ccgaggttgt cctccccggc
     8221 ctcggcctgc gcggcgctca tcccgtactt cttggtgagc atcgtgatca cgggctggat
     8281 gtcgtccttg gcgcggccgg ggtccagttc gaaggagcgg tgcaccacct cgacgtcctc
     8341 gcggtacggg aagtcccgca gcgccttctc gaagcgggcc ttgcccacgt agcaccaggg
     8401 gcaggcgatg tcgctccaga tctcgacgcg catgtctcgg ctctctccag gtcgtacggg
     8461 tacggagact ccctccgcca ggtgcatgaa cgttcaagca ggccgcttca ttccccgggc
     8521 aggacgaacc gcatgcgcag gtggtgatct cccggcccgg ccggttccgg gacccagccc
     8581 tgccgccggt agaacccctg cgcccgcacg ttgtccacgt gcacgtcgag cacggccgta
     8641 cgcctgccgt cggcctgcca ctcctccaca caggccgcgt gcagcgcgct gccgactcca
     8701 tggcgccagt ggtcggggtc gacgtggaac tggaacagtg ccaccgtgtc cgccggggtg
     8761 tcctcgggga cgcggaagga ggccaggccg acgatgcggc ccccctccac gacgcacagc
     8821 acccggccgt ccggccgggt gagggcgctg cgccagatgg cggaccagtc ggtgccgtcc
     8881 tgtggcagcc cgtcggggta gtacgtggcc cgggcccgtg cgtggaccgc gacgacgtcg
     8941 gccacctccg cgggcagggc ggtgcggatc acacggtcgg gggtcagtcg gtcgttgatc
     9001 atgcaaggga tgacgagacc ggccgggagg cggttcccgg ccggtcctca gctgccgtcg
     9061 cgcagatcgt ccggccaggc aggcctgaac tcgatgtggt cgaacgtcac cacgcagccc
     9121 tcccccatcg gggactgggt catgaagccg accagggccg cttccgtctc cttctcctcg
     9181 ccgagggtga agaggcggac gaaggtccag cgttcgccgt cgcgggaggc gtggaaggcg
     9241 aaggcgcggc cggtgcggct cacccgcagc cagacggaac tgccctccac ggtgaaggag
     9301 ttggcgtcgt cggagtgccc ccgggtcacc accgtgcaga cggtggggac gtccggggag
     9361 tactccaggc agagcttggc ccaggcccgc tcccctacat ggacgtagag caccccggcg
     9421 tcgaaggccg cgccgaaccc gacggtgacc ttggcgatca gctggaagtc gccctccggc
     9481 gccccgagca gccggggcgc gtcggaggcc gggtccagcc cttcaccggt gggcggcacg
     9541 aaccggtcct gccgcgcccc ggcccacccc gtgagcacgc cgtcctcgta ggaccagtgg
     9601 ccgtcgggcc catatgtgcg caggccgaag ggaagttcag aaaattccag atccattcgg
     9661 ccaagtgttt caggtcccac cctcaggggc gcggggaact gcgcgatcag ccacgacgag
     9721 cgtgcagccc gcagtccacc cgcaccccga cggcgtcaac gctccaaacg cccgttgaac
     9781 cgccgcggca gccccagcgg gttctcgtcc cgcaactcgg cgggaagcag cgcagacggc
     9841 gcgttctggt aggcaaccgg ccgcatccac cgctcgatcg ccgtcccgcc caccgacgtg
     9901 gacgtcgacg tcgtggccgg gtacggcccg ccgtggtgct gggccggggc gacggcgaca
     9961 cccgtaggcc agccgttcac caacacacgc ccggccagcg gcgtcagttc cgcgagaatc
    10021 tcggcgccac ggccctggcc ggcggcctcc tcctcggaca gctgcaccgt cgccgtgagg
    10081 ttccccggca gccgcgacag caccgacttc acctcggcct cgtcctcgta gcgggccacc
    10141 acggtgaccg gcccgaagca ctcctccagg agcaggtcgt gctcgccctc cgcggccagc
    10201 ctgctcgccg gcaccgtgag gaagccggcg ctgacggtgt gctcgccgcc cgcgccgggg
    10261 gtgaccgggg agtcgacgtc gggcagcccg gcccgctcgg cgacgccggc cacgaagttg
    10321 tcgcgcatgc ggtggtcgag caggaccccg gcgtcggtgt cgctgacggc gtcggtcagc
    10381 gacttcagca gggcgtcgc
//
