LOCUS       NZ_LTAO01000037        10399 bp    DNA     linear   CON 26-JAN-2024
DEFINITION  Alkalihalobacillus trypoxylicola strain KCTC 13244 scaffold6.1,
            whole genome shotgun sequence.
ACCESSION   NZ_LTAO01000037
VERSION     NZ_LTAO01000037.1
KEYWORDS    WGS; RefSeq.
SOURCE      Alkalihalobacillus trypoxylicola
  ORGANISM  Alkalihalobacillus trypoxylicola
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae;
            Alkalihalobacillus.
REFERENCE   1  (bases 1 to 192919)
  AUTHORS   Jeong,H., Park,S.H. and Choi,S.K.
  TITLE     Draft Genome Sequences of Four Plant Probiotic Bacillus Strains
  JOURNAL   Genome Announc 4 (3), e00358-16 (2016)
   PUBMED   27174273
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 192919)
  AUTHORS   Jeong,H., Park,S.-H. and Choi,S.-K.
  TITLE     Genome sequence of Bacillus trypoxylicola KCTC 13244(T)
  JOURNAL   Unpublished
REFERENCE   3  (bases 1 to 192919)
  AUTHORS   Jeong,H., Park,S.-H. and Choi,S.-K.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-FEB-2016) Super-Bacteria Research Center, KRIBB, 125
            Gwahak-ro, Yuseong-gu, Daejeon 34141, Korea, Republic of
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 25-FEB-2016
            Assembly Method                   :: A5-miseq v. 20140604
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 138.0x
            Sequencing Technology             :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/26/2024 01:26:58
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,153
            CDSs (total)                      :: 4,073
            Genes (coding)                    :: 4,035
            CDSs (with protein)               :: 4,035
            Genes (RNA)                       :: 80
            rRNAs                             :: 5, 2, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 5, 1, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 19 of 38
            Pseudo Genes (incomplete)         :: 25 of 38
            Pseudo Genes (internal stop)      :: 12 of 38
            Pseudo Genes (multiple problems)  :: 14 of 38
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 15:35:49
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 27414
            Orig. end                         :: 37813
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            LTAO01000037.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10399
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="ectoine"
                     /region_number="1"
                     /rules="ectoine_synt"
                     /tool="antismash"
     cand_cluster    1..10399
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="ectoine_synt"
                     /kind="single"
                     /product="ectoine"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10399
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5399](+)"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5399
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
     gene            587..1498
                     /locus_tag="AZF04_RS17705"
                     /old_locus_tag="AZF04_11460"
     CDS             587..1498
                     /GO_function="GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1010: NAD-dependent epimerase/dehydratase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003322938.1"
                     /locus_tag="AZF04_RS17705"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AZF04_11460"
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="WP_061949914.1"
                     /transl_table=11
                     /translation="MKIAITGGTGFIGKKLVQALLDEGHSVYVLTRSDRSNDSQGNLHY
                     IQWLQNHSYPEKELEGIDAFYHLAGESIGAKRWTEEQKEKILESRLSTTNEVIRILTEL
                     EKKPSALICASAIGYYGHSDHEVFTEESTPSQENFLTTVVKQWEESTQRAEELGIRVVS
                     CRLGIVLHPEEGALKKILLPIRFFIGGPLGKGTQWMSWIHIEDVIRLFVFVLKDLTISG
                     PINFTAPEPVKMKTFGKEIAQILNRPFWLPAPSFALKIALGEMSTLVLDGQKVMANKAF
                     SSDFSYKYTTVSEALNDLLTKQ"
     misc_feature    1509..1522
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: medium, score: 23.16"
                     /tool="antismash"
     gene            1812..2369
                     /locus_tag="AZF04_RS17710"
                     /old_locus_tag="AZF04_11465"
     CDS             1812..2369
                     /GO_function="GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003322939.1"
                     /locus_tag="AZF04_RS17710"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AZF04_11465"
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="WP_061949915.1"
                     /transl_table=11
                     /translation="MILKKRDVTDCHDLYHYLVDPAVFPYVRQKAFSYDEFIFLTKQTI
                     EQEERGELISRTICDEWGSPIGTINLFDISEHAGFLGTWIGKPFFGKGYNKLAKDVFFD
                     ELFYRCDIQTVYMRIRKENIRSILAAKKLPYSTLANEIRPDLYRQLNTAGDIYDLFQIE
                     KDHYYFHKIRTENELEQDAIEA"
     gene            2468..2596
                     /locus_tag="AZF04_RS21055"
     CDS             2468..2596
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="AZF04_RS21055"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="YfhE family protein"
                     /protein_id="WP_161485280.1"
                     /transl_table=11
                     /translation="MSKKKSNYDEKWNLRKALSVQYQSEFRAADRAAQSKNKNQND"
     gene            2987..3463
                     /gene="ectA"
                     /locus_tag="AZF04_RS17715"
                     /old_locus_tag="AZF04_11470"
     CDS             2987..3463
                     /EC_number="2.3.1.178"
                     /GO_function="GO:0033816 - diaminobutyrate
                     acetyltransferase activity [Evidence IEA]"
                     /GO_process="GO:0019491 - ectoine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="ectA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017187169.1"
                     /locus_tag="AZF04_RS17715"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AZF04_11470"
                     /product="diaminobutyrate acetyltransferase"
                     /protein_id="WP_268753434.1"
                     /transl_table=11
                     /translation="MHFSHPNKEDGAAMWQIVNETSLDQNSAYKYIMMADFFADTCLVA
                     KQNDELIGFVTAFRQPNQENTLFIWQIGVKPSAQGQGIASKLLKNLLKTEFSPEIRFIE
                     ATITPSNQASQALFKSLARDKKTECKIEPYFNEGLFPGDHHEEELMFRIGPVAR"
     gene            3573..4856
                     /gene="ectB"
                     /locus_tag="AZF04_RS17720"
                     /old_locus_tag="AZF04_11475"
     CDS             3573..4856
                     /EC_number="2.6.1.76"
                     /GO_function="GO:0045303 - diaminobutyrate-2-oxoglutarate
                     transaminase activity [Evidence IEA]"
                     /NRPS_PKS="Domain: Aminotran_3 (27-359). E-value: 6.6e-78.
                     Score: 254.0. Matches aSDomain:
                     nrpspksdomains_AZF04_RS17720_Aminotran_3.1"
                     /codon_start=1
                     /gene="ectB"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_3"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1013: aminotransferase class-III"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013488592.1"
                     /locus_tag="AZF04_RS17720"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AZF04_11475"
                     /product="diaminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="WP_061949917.1"
                     /sec_met_domain="Aminotran_3 (E-value: 2e-78, bitscore:
                     257.3, seeds: 13, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKPTDMNVFEKLESEVRSYCRSFPTVFSKAIGSKMWDESGKEYID
                     FFAGAGALNYGHNDPIMKSKLVDYILDDGITHSLDMASTAKGQFLETFNETILKPRNLN
                     YKIMFPGPTGTNTVESALKLARKATGRTDIISFTNGFHGMTIGALSVTGNSFKRKGAGI
                     PLHNVVTMPYDSFVHDKLDTLEYLERFLEDRGSGVAIPAAMILETVQGEGGINAASYEW
                     LQRIERICKRWGILLIIDDVQAGVGRTGTFFSFEKAGITPDIVCISKSIGGYGLPLALT
                     LIQPELDIWAPGEHNGTFRGNNHAFVTATAALSYWENPKFEASISKKSEKTKAFLEKLI
                     ADYPEIKGHFRGRGLMVGVCSEVEGFASKVAAEAFERGLIMETSGPEDEVFKLFPALTI
                     EDELLEKGFSIIEESVKAVVGQKEAIAN"
     aSDomain        3654..4649
                     /aSDomain="Aminotran_3"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_AZF04_RS17720_Aminotran_3.1"
                     /evalue="6.60E-78"
                     /label="AZF04_RS17720_Aminotran_3.1"
                     /locus_tag="AZF04_RS17720"
                     /protein_end="359"
                     /protein_start="27"
                     /score="254.0"
                     /tool="antismash"
                     /translation="SKAIGSKMWDESGKEYIDFFAGAGALNYGHNDPIMKSKLVDYILD
                     DGITHSLDMASTAKGQFLETFNETILKPRNLNYKIMFPGPTGTNTVESALKLARKATGR
                     TDIISFTNGFHGMTIGALSVTGNSFKRKGAGIPLHNVVTMPYDSFVHDKLDTLEYLERF
                     LEDRGSGVAIPAAMILETVQGEGGINAASYEWLQRIERICKRWGILLIIDDVQAGVGRT
                     GTFFSFEKAGITPDIVCISKSIGGYGLPLALTLIQPELDIWAPGEHNGTFRGNNHAFVT
                     ATAALSYWENPKFEASISKKSEKTKAFLEKLIADYPEIKGHFRGRGLMVGV"
     gene            5001..5399
                     /locus_tag="AZF04_RS17725"
                     /old_locus_tag="AZF04_11480"
     CDS             5001..5399
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     ectoine: ectoine_synt"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003322942.1"
                     /locus_tag="AZF04_RS17725"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AZF04_11480"
                     /product="ectoine synthase"
                     /protein_id="WP_045487887.1"
                     /sec_met_domain="ectoine_synt (E-value: 6.2e-56, bitscore:
                     181.2, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKVVKLADVIGTEHDVKNEDNTWNSRRLVLKKDGMGYSVHDTIIY
                     AGTETHIWYQNHLESVYCIEGEGEVETIADGKVWPIKKDEIYVLDKHDEHLLRAKTDMR
                     MVCVFNPPVSGKEVHDENGVYPVDDSDE"
     gene            complement(5602..6654)
                     /locus_tag="AZF04_RS17730"
                     /old_locus_tag="AZF04_11485"
     CDS             complement(5602..6654)
                     /EC_number="1.-.-.-"
                     /GO_function="GO:0010181 - FMN binding [Evidence IEA]"
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="other (smcogs) SMCOG1251: luciferase
                     family protein"
                     /gene_kind="other"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013312675.1"
                     /locus_tag="AZF04_RS17730"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AZF04_11485"
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="WP_061949918.1"
                     /transl_table=11
                     /translation="MELGLSTFVETTPTIGNGEKVTHAERIREVVEEIILADRVGLDVF
                     GVGEHHREDYAASSPAPILAAAAAQTKNIRLTSAVTVLSSADPVRVFQDFATVDAISNG
                     RAEIMAGRGSFIESFPLFGYDLQDYDQLFEEKLDLLLKITASEKVTWQGNFRPAIHDLG
                     IYPRPVQDPLPVWIGSGGNSESIARAGLLGLPLVLAIIGGNPKQFAPLVQLYKRAAEHA
                     GHNPNALTVASHSHGFVADTTEEAAEQFFPSTQYAMNKIGRERGWGPYTRAAFDSARSS
                     EGALYVGDPQTVADKIIDLRKNVGITRFMLHVPVGSMPHKQVMKAIQLLGEEVAPIVRE
                     EVAKWEEENK"
     gene            complement(6793..7089)
                     /locus_tag="AZF04_RS17735"
                     /old_locus_tag="AZF04_11490"
     CDS             complement(6793..7089)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003322946.1"
                     /locus_tag="AZF04_RS17735"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AZF04_11490"
                     /product="hypothetical protein"
                     /protein_id="WP_061949919.1"
                     /transl_table=11
                     /translation="MNLFKAHTVHPETDIPLVIYFNKRDGYMVFERDEEVITILKSVRL
                     DLWENDSFLQNLENITKLCKTQFPVQNFDDVYEILSLMGISKSDVQFKQMILH"
     gene            7340..8095
                     /locus_tag="AZF04_RS17740"
                     /old_locus_tag="AZF04_11495"
     CDS             7340..8095
                     /EC_number="1.1.1.320"
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1001: short-chain dehydrogenase/reductase SDR"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012957563.1"
                     /locus_tag="AZF04_RS17740"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AZF04_11495"
                     /product="(S)-benzoin forming benzil reductase"
                     /protein_id="WP_061949920.1"
                     /transl_table=11
                     /translation="MNYAIITGGSRGLGAAISKELLLENIHIISISRNENEELKTLAEA
                     NGGSFETIPFDLSLTEEIPSVMKEIFEKISLKRAEKIFLINNAGVVTPIKALHLADPME
                     ISDNIAINLIAPLLITSSFIYHTEKYAIDKRIINISSGSAQNPVYGWSSYGSSKAGINL
                     FSQTVAIEQKQLKHPTKICAFAPGVLDTDMQAEIRSTDKSDFTELARFQAYKAEGNLLS
                     ADTVATVISNLLYSEQFPNGKIVSINDYV"
     gene            complement(8191..9132)
                     /locus_tag="AZF04_RS17745"
                     /old_locus_tag="AZF04_11500"
     CDS             complement(8191..9132)
                     /NRPS_PKS="Domain: Abhydrolase_1 (73-183). E-value:
                     1.3e-16. Score: 52.9. Matches aSDomain:
                     nrpspksdomains_AZF04_RS17745_Abhydrolase_1.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF00561"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Abhydrolase_6"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF023568.4"
                     /locus_tag="AZF04_RS17745"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AZF04_11500"
                     /product="alpha/beta fold hydrolase"
                     /protein_id="WP_061949921.1"
                     /sec_met_domain="PF00561 (E-value: 5.2e-14, bitscore: 45.9,
                     seeds: 48, tool: rule-based-clusters)"
                     /sec_met_domain="Abhydrolase_6 (E-value: 1.2e-12, bitscore:
                     42.4, seeds: 455, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MDKEILDTLNKIKKEQVPFSLQQTKLNRTSIDQYLRFYHFQLKNI
                     SVRHNYGYLKVRQEQIFVQSFLPDNPKGTVFLLHGYLDHLGSLSSFIMFLLKKNYQVIG
                     FDMPGHGLSSGEKVSIQDFHDYVTVLDTIIEKVQKEISTPLFLVAHSTGAAVSFSFLSE
                     KNHLFKKIVLVAPLIRSASFHISHILYNVTRPFIRSIPRVYRSNSSEKAFIHFIKKDPL
                     QSKQLSMNWFGAKLKWFNQLKKGAVICKERVLIFQGNKDKTVDWRYNLHFLKKALPENQ
                     IYLIKGGKHHLLNEQMNIRQKVFEQIYQYFSN"
     aSDomain        complement(8584..8913)
                     /aSDomain="Abhydrolase_1"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_AZF04_RS17745_Abhydrolase_1.1"
                     /evalue="1.30E-16"
                     /label="AZF04_RS17745_Abhydrolase_1.1"
                     /locus_tag="AZF04_RS17745"
                     /protein_end="183"
                     /protein_start="73"
                     /score="52.9"
                     /tool="antismash"
                     /translation="TVFLLHGYLDHLGSLSSFIMFLLKKNYQVIGFDMPGHGLSSGEKV
                     SIQDFHDYVTVLDTIIEKVQKEISTPLFLVAHSTGAAVSFSFLSEKNHLFKKIVLVAPL
                     IRSASF"
ORIGIN
        1 aaaatgaata atttaacaat gcttgctcta taatcgaaga gttaactcct ttttgtacta
       61 gctcttgctt tactttgttc ggccctttta actgtgtatt cttctttgta gatatataac
      121 gttcagcaaa atctcgatca tctaataaat gttcttcgat tagttttttt ataactaact
      181 ctatatgttt gaccattctt cctttttcat ttaaatattt tctcatttct tcaatggaac
      241 gtattctgta cgataaataa tgaatggcaa ggtgataagt ttttttcatt tcgtcttcat
      301 ctataatttt ctctaattca gcatgagtta ttttaagacc ttttctaatt ccctttttaa
      361 tgagaacatc ttcatcaaca ctaaatccgt actcctcacc attaccttta tctataaata
      421 aatgataacg ctgtttgtat cttttatgat tggtaatttt tgttatagta atttccatgc
      481 tttcacctca gtttcttact ccttaagtat tccattaatt gcaggaaatc gtcaatcatt
      541 atagaatagg cgaaaggctt agcaactaat gaaaagaggg attatgatga aaattgccat
      601 aactggtggg accggcttta taggaaaaaa actagttcaa gctttacttg atgagggaca
      661 ttctgtttat gtcttaacaa gatccgatcg ttctaatgat agtcaaggaa atttacatta
      721 tattcaatgg ttacaaaacc actcatatcc tgaaaaagaa ttagagggca ttgatgcctt
      781 ttatcacctc gctggagaat ctatcggagc aaaacgatgg actgaggaac aaaaagaaaa
      841 aatacttgaa agtcgtcttt ccacaaccaa tgaggtcatc cgcattctca ctgagttaga
      901 gaaaaaacca tctgctctta tttgtgcttc cgctataggc tattatggtc attccgatca
      961 tgaagttttt acagaggaat ctactccgag tcaggaaaac tttttaacaa ccgtcgttaa
     1021 acaatgggaa gaatcaactc agagagctga agagttgggg attcgggttg tatcatgccg
     1081 tttaggaatt gttctacacc cagaagaagg cgctttaaag aaaatacttc tacctattcg
     1141 cttttttatt ggaggaccac ttggaaaagg gacccaatgg atgtcatgga ttcatataga
     1201 agatgttatt cgattatttg tatttgtttt aaaagatcta accattagtg gccctattaa
     1261 ttttacagca cccgagcctg taaaaatgaa gactttcgga aaggaaatag cccaaatatt
     1321 aaatcgcccc ttttggttac ccgctccatc atttgcatta aaaatagcat taggtgaaat
     1381 gagcacccta gtattagatg gtcaaaaggt gatggctaat aaggcatttt cctccgattt
     1441 ttcttataaa tacacaacgg ttagcgaagc cttaaatgat ttgttaacaa aacagtaaca
     1501 atggtaaatt tttttacaaa aaattttaaa aaaaatgtat cttttttaac aaaaaaacat
     1561 taatatatag aaggaaacaa gagtatttta acgaaatata tatagatagt agtagttaat
     1621 tcagtttaaa ccttatattt agctaaatat ctcaatattt tcaaccatat agcaattggg
     1681 aaggcaattg gtgcgccatc agtcaagctg gttctagtgg gttcgattcc caccccgaat
     1741 ttgataatga aagactatta aattcgaggg tggatccttt ttttgccctc gtcttacaag
     1801 gagggctttt tatgatctta aaaaaacgtg atgttacaga ttgccacgat ctttatcatt
     1861 atttagtcga cccggctgta tttccgtatg tgcgacaaaa agcattttca tacgatgaat
     1921 ttattttttt aactaaacag accattgaac aagaagaacg tggtgaatta atatctagaa
     1981 ccatttgtga tgaatggggt tccccaatag gaactataaa tctgtttgat atatcagagc
     2041 atgcaggttt tttaggaaca tggattggaa agcccttctt tggtaaagga tataataagc
     2101 tagctaagga tgtctttttt gatgagctat tttatcgttg tgatattcag acagtctata
     2161 tgagaattcg caaagaaaat attcgttcta ttttagccgc taaaaaacta ccttacagta
     2221 cactagcgaa tgaaattaga ccggatttat acagacagct caacacagct ggcgatattt
     2281 atgatttgtt tcaaattgaa aaagatcatt attatttcca caaaatccgt acagaaaacg
     2341 agctagagca agatgcgatt gaagcatagt ttggttgcta agttttaaat taaagtctcc
     2401 tttatttttt gtatgaaatc tcttgctttt ggtaatgact aaaaaaagcg agaagggaga
     2461 ttatcacatg tcaaaaaaga aaagcaacta tgacgaaaaa tggaatctac ggaaagccct
     2521 gtccgtccaa tatcaatcgg aatttcgtgc tgccgaccga gcagctcaat caaagaataa
     2581 aaatcaaaat gactaatttt taaaaagtcc tatatagggc ttttttttat atttcattct
     2641 ttttttaaat aattggtacg ttttattcaa atgataacgt cggatattag cgaatgaatg
     2701 tatttttaat aagtcttttt tcaatttgta agaatctcta acaaaatagg aggatattat
     2761 acgatactac tccttagagt gattaagata tcctaatcca tgtattagct ctgttatacg
     2821 cttatagctc atttgcacca ttgaacgctt acacttaaga tataatatgt ggtttgaaaa
     2881 acttatacac gggtattaaa aggttgaagt gaaataaaac agattaaagg aggagtccat
     2941 cattagtagt caacaaacga ttacgccagt aaaagacaaa gaacttatac atttttctca
     3001 tcccaataaa gaagatggtg ccgcgatgtg gcaaattgtt aatgaaactt cattagacca
     3061 aaattcagcc tataaatata ttatgatggc cgattttttt gcagacactt gtttagttgc
     3121 caagcagaat gacgaactga ttggttttgt aacagccttt cgccagccta atcaagaaaa
     3181 tactttattt atctggcaaa ttggagtaaa accaagtgct caaggacaag gtatcgcatc
     3241 taaattattg aaaaatttat taaagactga attttcacca gaaattagat ttatagaagc
     3301 cactataact ccttctaacc aagcctctca agcattattt aaaagcttag cgcgagataa
     3361 aaaaaccgaa tgtaaaatag aaccgtattt taacgaaggg cttttcccag gagatcatca
     3421 tgaagaagag ttgatgtttc gaataggacc ggttgcccgt tagaataagt aaaaatcaaa
     3481 tgttttatta ttatgtatgt ttttgtacat ctactaatat actcatacac agtattagta
     3541 tattgattat aaataggagg agattataaa taatgaaacc taccgatatg aacgtttttg
     3601 aaaaactaga atcagaggtt cgtagttatt gtagaagctt tcctactgtc ttttcaaaag
     3661 caataggatc aaaaatgtgg gatgaatctg ggaaggaata tattgatttc tttgccggag
     3721 caggagcatt aaattatggg cataatgacc ctatcatgaa atcaaaatta gtggactata
     3781 ttttagatga tggaattact cactcattag acatggcttc gacagcaaaa ggacaatttt
     3841 tagagacctt caacgaaacc attttgaagc caagaaatct taattataaa atcatgttcc
     3901 caggtccaac aggaacaaat acggtagaaa gtgcacttaa attagcacgt aaagcaacag
     3961 gtcgtactga tattatcagt tttactaatg gatttcatgg tatgactatc ggcgctttat
     4021 ctgttactgg taattcattt aaacgaaaag gagcaggtat tcctctccat aatgtagtta
     4081 cgatgcctta tgatagtttt gtacatgata aactcgacac tctggagtat ctcgaacgct
     4141 ttttagaaga tcgtggaagt ggagtagcga tcccagctgc tatgatcctc gagacagtcc
     4201 aaggtgaagg tggtataaat gccgcaagct acgagtggtt acaaagaatc gaaagaatat
     4261 gtaaacgatg gggtatctta ttaattattg atgatgttca ggcaggtgta ggacgaacag
     4321 gaactttctt tagttttgaa aaagcaggaa ttacacctga tattgtgtgt atttcaaaat
     4381 caatcggagg ttatggatta ccattagctt taactttaat ccaacctgag ctcgatattt
     4441 gggcgcctgg tgaacataat ggtacattcc gagggaataa ccatgcattt gtcacagcta
     4501 cggctgcact atcttattgg gagaatccaa agttcgaagc aagtatttca aaaaaatcag
     4561 agaaaacaaa ggctttttta gaaaaattaa tagctgatta tcctgaaata aaaggacatt
     4621 tccgaggacg cggattaatg gtaggtgtgt gctcagaggt tgaaggattc gcctcaaaag
     4681 tagcagcaga agcatttgag cgtggattaa ttatggaaac atctggtcca gaagatgaag
     4741 tctttaagct cttccctgct ttaacaattg aagatgagtt actagaaaaa ggattttcca
     4801 taatagagga aagtgtaaaa gcggtcgttg gacaaaaaga agcgattgca aactaattaa
     4861 tttatcagaa taaacatcaa aacaattttg aatgattcag aatttagcag ttagcagaga
     4921 caaacattct ctatgtaagt gacaaaaaaa gaatgattag tcctgtatgc tacatgtaac
     4981 taacgatgga ggtatgtaaa atgaaagtcg tcaaactcgc tgatgtaatt ggaacagaac
     5041 atgatgtaaa aaatgaagat aatacatgga atagccgacg tctcgtatta aaaaaagatg
     5101 gaatggggta ttccgtacac gatacaatta tttacgcagg aacagaaaca catatttggt
     5161 atcaaaatca ccttgaatca gtctattgca ttgaaggtga aggcgaggtc gaaacaatag
     5221 cagatggtaa agtttggcct ataaaaaagg atgagattta tgttttagat aaacatgatg
     5281 agcatctttt acgagctaaa acggatatga gaatggtttg tgtttttaac ccccctgttt
     5341 ctggaaaaga agtgcacgat gaaaatggag tttatccagt agacgattca gatgaataaa
     5401 ataggtcatt tatttccaat taaataagaa cagcgggtaa aatcatcaac tggtgattta
     5461 cccgctgttt tcattattgg ggtatgaacg ctatcaaaaa gcattttttc aaaactttat
     5521 agaaaaaatg atgaccattt aacatttaaa tagtcatcgg gcttttccaa tatatacttt
     5581 tatccaacct ctttaatatg attacttatt ctcttcttcc cactttgcta cctcttctcg
     5641 aacgatagga gccacttctt caccaagcaa ttgaatggct ttcataactt gtttatgagg
     5701 catagagcca acgggaacat gtagcataaa ccgtgtgatc ccaacatttt ttcttaaatc
     5761 aataatttta tctgcaaccg tttgtgggtc acctacatat aaagctcctt ctgaggacct
     5821 tgccgaatca aaagcagctc tagtatatgg tccccatcct ctttctcttc caattttatt
     5881 catcgcatat tgagtagaag gaaagaattg ttcagctgct tcctctgtag tatcagccac
     5941 aaaaccatgt gagtgggagg caaccgttag agcatttggg ttatgacctg catgctcagc
     6001 tgctcgctta tatagttgaa ctaatggtgc gaattgtttt ggattcccac cgataatcgc
     6061 taatacaagt ggaagtccta atagcccagc tctagcaatg gactctgaat tcccaccact
     6121 ccctatccat acaggaagag gatcttgtac aggtctcgga taaattccta aatcatgaat
     6181 ggccggtcta aaattccctt gccaagttac cttctcagaa gccgtaattt ttaaaagcaa
     6241 atctaatttt tcttcaaaaa gttgatcata atcctgtaaa tcatatccaa atagagggaa
     6301 tgattctata aatgaacctc taccagccat aatttcagct ctaccatttg aaattgcatc
     6361 tacagtagca aaatcctgaa aaaccctaac aggatcagca gaagaaagga cagtaacagc
     6421 acttgttagg cgaatattct tcgtttgtgc agccgccgcc gccaatatag gtgctggaga
     6481 tgaggccgca taatcttctc tgtgatgctc ccctactcca aatacatcaa gacctacccg
     6541 atcagctaat ataatctcct caaccacttc tctaattctt tctgcatgag taaccttttc
     6601 tccattacca atggtcggtg ttgtttcaac gaaggtactg agccctaatt ccattataaa
     6661 tccccctatt aatttttact tactttttta tattaacact atttttatca taaataaact
     6721 tttttgaata aacagtagaa ctttgttcga taagatgtct tttaaacgag aggcttaaat
     6781 gaatttaatt agttaatgca gaatcatctg cttaaattgt acatcggatt tcgaaattcc
     6841 cattaatgaa aggatttcat agacatcgtc aaaattttgc actggaaatt gtgttttaca
     6901 tagcttcgta atattttcta agttttgtaa aaaagaatca ttttcccaga ggtccaatct
     6961 aacacttttt aaaatagtaa tgacttcctc atctctctca aataccatat atccatctct
     7021 tttattaaaa taaatgacta gaggaatatc ggtctctgga tggaccgtat gggctttaaa
     7081 taaattcatt aaattccaac tccctaaact ttctttattt ttctattatt tatcatatgg
     7141 gattttcata atgaatcaag aaaatggtcc atttatttcc tcttaaaata aatctagtag
     7201 taatcaaaag caatatgata gactatttag tgtactataa catgtctaaa tgtccggaag
     7261 aaataatggt acatatctca ttttgtagaa atactttatt ggttctttcc actattgtaa
     7321 aaaggggtgc atcaaggtaa tgaattatgc aataattact ggtggttcta gaggcttagg
     7381 agcagctata agtaaagagc ttttattaga gaacattcac attatatcaa tttctcgtaa
     7441 tgaaaatgaa gaactaaaaa cgctagccga agcaaacgga ggttcatttg aaacgattcc
     7501 ttttgattta agtttaacgg aggaaatacc ctctgttatg aaggaaatat ttgaaaagat
     7561 cagtttaaaa cgagccgaaa aaatctttct tatcaataat gctggagtag ttacacccat
     7621 aaaggcactg cacctagcag acccaatgga aattagcgat aatattgcta ttaatttaat
     7681 agcgccttta cttattactt caagctttat ttatcataca gaaaaatatg ccatcgataa
     7741 gcgtatcatt aatatctctt ctggttcagc tcaaaatcct gtgtatggat ggagtagcta
     7801 tggaagttca aaggctggta ttaatctctt tagccaaact gttgcgattg aacaaaaaca
     7861 attaaaacac cctactaaaa tttgcgcttt tgctcctggg gtacttgata ctgatatgca
     7921 agccgaaatt cgttctacgg ataaatcaga tttcacagag ctagcaagat ttcaagcata
     7981 taaagcagaa ggaaatcttc tatcggccga tacggttgca accgttattt ctaatttgct
     8041 atatagtgaa caatttccta acggaaaaat tgtgagcatc aatgattatg tgtaacttta
     8101 taaacgttca gtgggggctc cccccattga atgtttatgt tctcatgttg attaagtaat
     8161 tccccaccta tgctctaaca cgtacatctc ctaatttgaa aaatattgat aaatttgttc
     8221 aaatactttc tgtctaatat tcatttgctc atttaaaagg tgatgcttac caccttttat
     8281 taaatagatt tgattttctg gtaaagcctt ttttaaaaag tgtaggttat accgccaatc
     8341 cactgtttta tctttgttac cttgaaagat aagtacacgc tctttgcaaa taaccgcccc
     8401 cttttttaat tgattaaacc actttaactt tgccccaaac cagttcattg aaagctgttt
     8461 actttgtaaa gggtcttttt ttataaaatg aataaatgcc ttctcacttg aattagaacg
     8521 atacactcga ggaatacttc gtatgaatgg tcttgtaacg ttatataaaa tatgtgagat
     8581 atgaaaagag gctgaacgta ttaatggagc aacaagaaca atttttttaa agagatgatt
     8641 tttttctgaa agaaaactaa agctaacagc ggccccagta ctatgtgcga ccaaaaataa
     8701 aggggtagaa atctccttct gtactttttc tataatcgta tcaagtactg taacataatc
     8761 atgaaagtct tgaatactaa ctttctctcc tgatgataat ccatgtcctg gcatatcgaa
     8821 gccaattacc tgataatttt ttttcaataa aaacatgata aatgaactta aactaccaag
     8881 gtgatccaaa tacccatgta ataaaaaaac agtgccttta gggttgtctg gtaaaaagga
     8941 ttgaacgaat atttgctcct gtcttacttt taaataacca taattatgtc gaacagatat
     9001 attttttaat tgaaaatgat aaaatcgtaa atattggtct atagaagtcc gattcagttt
     9061 cgtttgttgt agggaaaaag gcacttgttc tttcttaatc ttattaagtg tatcaagtat
     9121 ttctttatcc atttaaccca ctcctattgt tcattcttgt ttatattccc tctatgagag
     9181 aatttaaatg ctcttttcgt aattattttg tttttttagt aagaaaatat acaaactact
     9241 aatcacgatg gaacatttaa cgacaaaaaa gcctctaaga aagggatttc cctccttaaa
     9301 ggcctaatat ttcgaaaata tacattggta cgattagaat tgtaataaat atcctattta
     9361 ataaacataa tatggtatat ttaaactata aaaaggaaag aacatactct attatagcta
     9421 gtttaatttt ttctcactat gaacccattt caccattctt atcttttatt gaaaaacgaa
     9481 tgatagagat ttttgatttt cactttaggt agatgctttc ccaagggctt gtcagctaac
     9541 tccgactact tagagcgccg taagagtggg tcttcaaacg gcgctcaccc tttgagagtc
     9601 actacggtca tcggtttcgt ccacttaaaa acaaagataa aactggactt actataaata
     9661 caaaaaaaaa caaaaaccca aaggctatta aattcccttg ggtctatcga atgatagatt
     9721 cataaaaaat gctctattaa actttattta agtcatgtaa aattaaatta ttaactacgc
     9781 tagttgtatg tttggcaagg aatatcttct aaaaaatcac gtatgagcta atggtagcat
     9841 gaaaggtaca ataattccta ctgaagcaac gaaccttaat aagtgatact ttttcatatt
     9901 ccttcacctc cttagtctat attttacata actcgtctta attcagcaaa acgagaagaa
     9961 ttacgattaa cccctacttt ttctttactt ttttgacaat ttcgggcatt ttcttctcct
    10021 tatattaaaa ttttaatatg tttagtgagg gatcgttatg gacagacata ttggtaaaaa
    10081 acttcaagaa ttaagacatt attataatgt cagtcaaaaa gacctagctg aaggaatatg
    10141 taatcaaagt aatatcgccc acatagaaaa tgggggaaac atacctaacg cagaaattct
    10201 cttcaaatta gcgaaaaagt taggtgttac cattaattat ttctttgatg attatgaagc
    10261 tcctcgttca aactatgtac aagatacttg tgaattaatc aggcaaggaa ttagagagta
    10321 taaatttaag gaaacccttc aaattataaa aaaagaatat aaaaatccgt tatttaaaaa
    10381 agtagaatta aagaaattt
//
