LOCUS       NZ_LQYL01000009         9329 bp    DNA     linear   CON 26-JAN-2024
DEFINITION  Bacillus licheniformis strain B4090_201601 NODE_14, whole genome
            shotgun sequence.
ACCESSION   NZ_LQYL01000009
VERSION     NZ_LQYL01000009.1
KEYWORDS    WGS; RefSeq.
SOURCE      Bacillus licheniformis
  ORGANISM  Bacillus licheniformis
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus.
REFERENCE   1  (bases 1 to 9329)
  AUTHORS   Krawczyk,A.O., de Jong,A., Holsappel,S., Eijlander,R.T., van
            Heel,A., Berendsen,E.M., Wells-Bennik,M.H. and Kuipers,O.P.
  TITLE     Genome Sequences of 12 Spore-Forming Bacillus Species, Comprising
            Bacillus coagulans, Bacillus licheniformis, Bacillus
            amyloliquefaciens, Bacillus sporothermodurans, and Bacillus
            vallismortis, Isolated from Foods
  JOURNAL   Genome Announc 4 (3), e00103-16 (2016)
   PUBMED   27174261
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9329)
  AUTHORS   Berendsen,E.M., Wells-Bennik,M.H., Krawcyk,A.O., De Jong,A.,
            Holsappel,S., Eijlander,R.T. and Kuipers,O.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-JAN-2016) Molecular Genetics, University of Groningen,
            Nijenborgh 7, Groningen, Groningen 9747AG, the Netherlands
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2015
            Assembly Method                   :: Velvet v. 1.2.10
            Assembly Name                     :: B4090
            Genome Coverage                   :: 100.0x
            Sequencing Technology             :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/26/2024 01:23:23
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,545
            CDSs (total)                      :: 4,463
            Genes (coding)                    :: 4,342
            CDSs (with protein)               :: 4,342
            Genes (RNA)                       :: 82
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 121
            CDSs (without protein)            :: 121
            Pseudo Genes (ambiguous residues) :: 0 of 121
            Pseudo Genes (frameshifted)       :: 68 of 121
            Pseudo Genes (incomplete)         :: 58 of 121
            Pseudo Genes (internal stop)      :: 20 of 121
            Pseudo Genes (multiple problems)  :: 22 of 121
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 10:24:46
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 0
            Orig. end                         :: 9329
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            LQYL01000009.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     gene            <1..1615
                     /locus_tag="B4090_RS05170"
                     /old_locus_tag="B4090_0459"
     source          1..9329
                     /collected_by="TIFN project"
                     /collection_date="2012-01-10"
                     /country="Netherlands"
                     /db_xref="taxon:1402"
                     /isolation_source="Pea soup (8h 40 C degree)"
                     /mol_type="genomic DNA"
                     /organism="Bacillus licheniformis"
                     /strain="B4090_201601"
                     /submitter_seqid="NODE_14"
     region          1..9329
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="NRPS"
                     /region_number="1"
                     /rules="cds(Condensation and (AMP-binding or A-OX) and not
                     alpha_am_amid)"
                     /tool="antismash"
     cand_cluster    1..9329
                     /SMILES="NC(C(C)C)C(=O)NC(CC(=O)O)C(=O)O"
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="cds(Condensation and (AMP-binding or
                     A-OX) and not alpha_am_amid)"
                     /kind="single"
                     /product="NRPS"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..9329
                     /aStool="rule-based-clusters"
                     /category="NRPS"
                     /contig_edge="True"
                     /core_location="[1634:>9329](+)"
                     /cutoff="20000"
                     /detection_rule="cds(Condensation and (AMP-binding or A-OX)
                     and not alpha_am_amid)"
                     /neighbourhood="20000"
                     /product="NRPS"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      1635..>9329
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="cds(Condensation and (AMP-binding or A-OX)
                     and not alpha_am_amid)"
                     /neighbourhood="20000"
                     /product="NRPS"
                     /protocluster_number="1"
     CDS             <3..1615
                     /GO_function="GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /NRPS_PKS="Domain: PCP (0-64). E-value: 2.7e-26. Score:
                     83.5. Matches aSDomain: nrpspksdomains_B4090_RS05170_PCP.1"
                     /NRPS_PKS="Domain: Epimerization (75-377). E-value:
                     4.9e-168. Score: 552.3. Matches aSDomain:
                     nrpspksdomains_B4090_RS05170_Epimerization.1"
                     /NRPS_PKS="Domain: TIGR01720 (382-533). E-value: 4.4e-49.
                     Score: 158.2. Matches aSDomain:
                     nrpspksdomains_B4090_RS05170_TIGR01720.1"
                     /codon_start=2
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PP-binding"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Condensation"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1127: condensation domain-containing protein"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020450105.1"
                     /locus_tag="B4090_RS05170"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="B4090_0459"
                     /product="condensation domain-containing protein"
                     /protein_id="WP_235598151.1"
                     /sec_met_domain="PP-binding (E-value: 9.9e-21, bitscore:
                     61.4, seeds: 164, tool: rule-based-clusters)"
                     /sec_met_domain="Condensation (E-value: 1.4e-85, bitscore:
                     274.0, seeds: 42, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="LADIWQDVLGIDRIGVTDNFFALGGDSIKGIQMASRLQQHGWKLE
                     MKDLFQHPTIGELSAYVQAADDQPIDQNPVEGEVTLTPIQRWFFERKFTNEHHWNQSVM
                     LHALSGFDPEAAGKALTKLIEHHDALRMVYQRNGDGIVQYNRGLEETAVRPEVIRFNES
                     GAELEAAVLKESNRIQSSINLTEGPLLKAAIFETDQGDHLLIVIHHLVVDGISWRILLE
                     DFAAGYAQAEKGEPIILQEKTHSFAEYAARLKEYARSKAFAKEIGYWQEVEKAETASLP
                     KDDEAEDKRMHHTKTAEFSLSKEETEQLMTKVHEAYNTEMNDILLTALGLALKEWTGQE
                     DFIICLEGHGREDIMEGLNISRTVGWFTSQFPALIQLRHSEDIGYQIKQIKEELRHIPN
                     KGIGYGIYRYLTEEGKKAQPIKHDISFNYLGQFAEMADSGLFTRSALPSGDPLSPETEK
                     PNALDIVGYIENGILTMSIAYHSLEYKESTVAAVAESFKTYLLQLIDHCLERDGGELTP
                     SDLGDDELTLEELDKLMEIF"
     aSDomain        5..196
                     /aSDomain="PCP"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_B4090_RS05170_PCP.1"
                     /evalue="2.70E-26"
                     /label="B4090_RS05170_PCP.1"
                     /locus_tag="B4090_RS05170"
                     /protein_end="64"
                     /protein_start="0"
                     /score="83.5"
                     /tool="antismash"
                     /translation="LADIWQDVLGIDRIGVTDNFFALGGDSIKGIQMASRLQQHGWKLE
                     MKDLFQHPTIGELSAYVQA"
     aSModule        5..1603
                     /domains="nrpspksdomains_B4090_RS05170_PCP.1"
                     /domains="nrpspksdomains_B4090_RS05170_Epimerization.1"
                     /domains="nrpspksdomains_B4090_RS05170_TIGR01720.1"
                     /incomplete
                     /locus_tags="B4090_RS05170"
                     /tool="antismash"
                     /type="unknown"
     CDS_motif       59..88
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_B4090_RS05170_0001"
                     /evalue="3.70E-03"
                     /label="PCP_mC"
                     /locus_tag="B4090_RS05170"
                     /protein_end="28"
                     /protein_start="18"
                     /score="10.7"
                     /tool="antismash"
                     /translation="NFFALGGDSI"
     aSDomain        230..1135
                     /aSDomain="Epimerization"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_B4090_RS05170_Epimerization.1"
                     /evalue="4.90E-168"
                     /label="B4090_RS05170_Epimerization.1"
                     /locus_tag="B4090_RS05170"
                     /protein_end="377"
                     /protein_start="75"
                     /score="552.3"
                     /specificity="E activity: active"
                     /specificity="Active site motif: HHLVVDG"
                     /tool="antismash"
                     /translation="EGEVTLTPIQRWFFERKFTNEHHWNQSVMLHALSGFDPEAAGKAL
                     TKLIEHHDALRMVYQRNGDGIVQYNRGLEETAVRPEVIRFNESGAELEAAVLKESNRIQ
                     SSINLTEGPLLKAAIFETDQGDHLLIVIHHLVVDGISWRILLEDFAAGYAQAEKGEPII
                     LQEKTHSFAEYAARLKEYARSKAFAKEIGYWQEVEKAETASLPKDDEAEDKRMHHTKTA
                     EFSLSKEETEQLMTKVHEAYNTEMNDILLTALGLALKEWTGQEDFIICLEGHGREDIME
                     GLNISRTVGWFTSQFPALIQL"
     CDS_motif       245..274
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_B4090_RS05170_0002"
                     /evalue="3.20E-05"
                     /label="NRPS-E1"
                     /locus_tag="B4090_RS05170"
                     /protein_end="90"
                     /protein_start="80"
                     /score="16.5"
                     /tool="antismash"
                     /translation="LTPIQRWFFE"
     CDS_motif       335..406
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_B4090_RS05170_0003"
                     /evalue="3.80E-05"
                     /label="C2_LCL_024-062"
                     /locus_tag="B4090_RS05170"
                     /protein_end="134"
                     /protein_start="110"
                     /score="16.1"
                     /tool="antismash"
                     /translation="FDPEAAGKALTKLIEHHDALRMVY"
     CDS_motif       611..676
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_B4090_RS05170_0004"
                     /evalue="1.40E-11"
                     /label="NRPS-E2"
                     /locus_tag="B4090_RS05170"
                     /protein_end="224"
                     /protein_start="202"
                     /score="36.5"
                     /tool="antismash"
                     /translation="LLIVIHHLVVDGISWRILLEDF"
     CDS_motif       1079..1126
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_B4090_RS05170_0005"
                     /evalue="1.30E-07"
                     /label="NRPS-E5"
                     /locus_tag="B4090_RS05170"
                     /protein_end="374"
                     /protein_start="358"
                     /score="23.8"
                     /tool="antismash"
                     /translation="NISRTVGWFTSQFPAL"
     aSDomain        1151..1603
                     /aSDomain="TIGR01720"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_B4090_RS05170_TIGR01720.1"
                     /evalue="4.40E-49"
                     /label="B4090_RS05170_TIGR01720.1"
                     /locus_tag="B4090_RS05170"
                     /protein_end="533"
                     /protein_start="382"
                     /score="158.2"
                     /tool="antismash"
                     /translation="IGYQIKQIKEELRHIPNKGIGYGIYRYLTEEGKKAQPIKHDISFN
                     YLGQFAEMADSGLFTRSALPSGDPLSPETEKPNALDIVGYIENGILTMSIAYHSLEYKE
                     STVAAVAESFKTYLLQLIDHCLERDGGELTPSDLGDDELTLEELDKL"
     CDS_motif       1172..1234
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_B4090_RS05170_0006"
                     /evalue="3.80E-10"
                     /label="NRPS-E6"
                     /locus_tag="B4090_RS05170"
                     /protein_end="410"
                     /protein_start="389"
                     /score="31.9"
                     /tool="antismash"
                     /translation="IKEELRHIPNKGIGYGIYRYL"
     gene            1635..>9329
                     /gene="licB"
                     /locus_tag="B4090_RS05175"
     CDS             1635..>9329
                     /GO_function="GO:1904091 - non-ribosomal peptide synthetase
                     activity [Evidence IEA]"
                     /NRPS_PKS="Domain: NRPS-COM_Nterm (1-29). E-value: 2e-15.
                     Score: 47.7. Matches aSDomain:
                     nrpspksdomains_B4090_RS05175_NRPS-COM_Nterm.1"
                     /NRPS_PKS="Domain: Condensation_DCL (6-306). E-value:
                     9.7e-114. Score: 371.4. Matches aSDomain:
                     nrpspksdomains_B4090_RS05175_Condensation_DCL.1"
                     /NRPS_PKS="Domain: AMP-binding (470-862). E-value:
                     1.8e-113. Score: 371.2. Matches aSDomain:
                     nrpspksdomains_B4090_RS05175_AMP-binding.1"
                     /NRPS_PKS="Domain: PCP (967-1035). E-value: 2.3e-24. Score:
                     77.3. Matches aSDomain: nrpspksdomains_B4090_RS05175_PCP.1"
                     /NRPS_PKS="Domain: Condensation_LCL (1050-1337). E-value:
                     1.9e-104. Score: 340.8. Matches aSDomain:
                     nrpspksdomains_B4090_RS05175_Condensation_LCL.1"
                     /NRPS_PKS="Domain: AMP-binding (1502-1905). E-value:
                     1.3e-112. Score: 368.4. Matches aSDomain:
                     nrpspksdomains_B4090_RS05175_AMP-binding.2"
                     /NRPS_PKS="Domain: PCP (2012-2081). E-value: 2.3e-23.
                     Score: 74.2. Matches aSDomain:
                     nrpspksdomains_B4090_RS05175_PCP.2"
                     /NRPS_PKS="Domain: Condensation_LCL (2096-2382). E-value:
                     1.6e-102. Score: 334.5. Matches aSDomain:
                     nrpspksdomains_B4090_RS05175_Condensation_LCL.2"
                     /codon_start=1
                     /gene="licB"
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     AMP-binding"
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     Condensation"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PP-binding"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1002: AMP-dependent synthetase and ligase"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020450106.1"
                     /locus_tag="B4090_RS05175"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="lichenysin non-ribosomal peptide synthetase LicB"
                     /protein_id="WP_061576348.1"
                     /sec_met_domain="Condensation (E-value: 3.4e-90, bitscore:
                     289.2, seeds: 42, tool: rule-based-clusters)"
                     /sec_met_domain="PP-binding (E-value: 2e-18, bitscore:
                     53.9, seeds: 164, tool: rule-based-clusters)"
                     /sec_met_domain="AMP-binding (E-value: 1.8e-120, bitscore:
                     389.3, seeds: 400, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MGKEKIQKVYPLTPMQEGMLYHAMLDPESSSYFTQLELVIDGEFD
                     LGIFEKSVNQLVRSYDILRTVFVHQQLQKPRQVVLAERQAIVEFEDLADLDEGRQKTRI
                     DQYKQDVQAAGFNLAKDMLFKTAVFRLDRKKHHLVWSNHHIVMDGWSMGILMKRLFQNY
                     EAFRANRTVTLDQGKPYADYIKWLGRQDKDEAASYWEHRLSGLESPSVIPGGKTQPDSG
                     SYRNEEFTFVWDKDMVDALLKTANRYHVTAPNLFQAIWGAVLAKYNRTDDVVFGTVVSG
                     RPSEIDGIEHMAGLFINTIPVRLKIDKNASFAELFKQAQQHAIDAERYDYVPLYEIQKQ
                     SALAGRLISHLVAFENYPLDKELENGTIHERLGFSIKAAGAFEQTNYDFNLIVYPGAEW
                     TIKLKYNGAAFDAAFIEQAAGHLTRLAEEAIANPESKAVSASMLSKQEQQTLLAFNQTK
                     TGYPKEKTIPELFEEQVEASPDKTAVAADGRSLTYRRLNEKANQLARKLKAKGLMHGNA
                     AAILMERSLEAAVSMLAVLKAGGVYVPIDPEYPEERIRFLLEDSGAKIVLTKDSPQISL
                     EGYEVLAANAVDAEKEDTANLVHANKPEDLAYYIYTSGSTGKPKGVMVEHRNIVRLVKN
                     AGYIPLKSDVKMAQTGAVSFDASTFEVFGALLNGGTLYPVPKETLLDGKRFRVFLEETG
                     ITTMWLTSPLFNQLAQQDPGMFATLSDLIIGGDALVPGIVNRVKRESPELSLWNGYGPT
                     ENTTFSTCFFIDQAYERTIPIGKPIGNSTAYIVDEHGALQPIGVPGELCVGGDGVARGY
                     LNQPELTDEKFVGDPFAEGKRMYRTGDLAKWLPDGNIEFLGRIDHQVKVRGFRIELGEV
                     EAALARLEGISEAAAVIRENNTGETEICAYYTAVGARPPAQLRTELSRSLPEYMIPAHL
                     IELDSMPLTANGKVDKRQLPAPIAEETDRFEAPKNETEKILAAIWEEILGIKQPGIDDN
                     FFSIGGHSLKAMMLTAKIQEQLQKEVPIKVLFEKPTIRELAEFLQGETKEKTAPIAPAP
                     SRANYPVSSAQRRMYILNQLEEASTSYNVPAVLLLEGELDKERLEDAFRALISRHETLR
                     TSFELIDGEIVQTIHQDAVFHLTESVADEAEAEAAIRGFIRPFQLNKAPLVRAELVKLE
                     EKRHLLMIDMHHIITDGSSTGILIRDLASFYHGEEAPAPKLHYKDFAVWQNGPEHQEKL
                     KEQEQYWLDIFKGELPVLDLPYDFPRPSKRSFEGDRVVFGINRKLTADVQKLLSEADAT
                     LYMFLLAAFNILLSKYASQEDLIVGSPVAGRTHPDLHNIPGMFVNTLALRNRPEGEKTF
                     KEFLQEVKETSLQGFAKQDYPLEELIEKLPLLRDTSRNPLFSVFFNMQNMEIPTLKLGD
                     LQISSYSVRHRTAKFDLSLEAVEHDGEIGLSFDYAVSLFKEETIRRWSGHFLNVIKTIC
                     ENPGIKLRDIELLSDKERGLLLEEVRRSRAERLEERPFHVRFEEKAAEMPDQPAVVCGE
                     TILTYRGLDERANQIANVLRSEGIGKDDVVGIMLDRSAEVAAAILGVMKSGGAFLPIDP
                     EMPTSRIQYMLEDSKARWLLTEHSHQAALADWYNGRLIDVRNDTLMASKKRPHLIHDGA
                     SLAYIIYTSGTTGQPKGVQLEHRNLANYVSWFISEARLSASDKTMLLSSYAFDLGYTSL
                     FPVLLAGGELHIVPKETYTEPETFIHYIGEQGITYIKLTPSLFHTVVQPQSFALAKGLQ
                     SLRLIVLGGEKINPKDVERFHSRYPDTRFINHYGPTETTIGAIAKPVEPGMIRQFAERP
                     TIGRPISGAGALVLDASRRLVPAGAAGELYITGSGLARGYVNNRELTAAKFIENPYTPG
                     TFMYRTGDLVRRLPNGEIEFLGRTDDQVKIRGYRIELKEIEEAFIGLEHIERAVVLSFT
                     ADSGLDELCAYIQAKRQLPVSELRERLSERLPSYMIPSYFVTVDKMPLAANGKVDRSAL
                     PEPQCLSGQSDSYRAPSTEFEKALCGIWEEVLGVRPVGLDDNFFDLGGHSLKGMMLTSE
                     IQSKLGKKVPLKTLFDCPTAGLLARSIEADGQAEQSGIQPAEKRDWYPVSSAQQRMYAL
                     HHIEKNGTGYNMPSVLMLEGVLDTDRLRRAFADLVNRHETLRTAFVEIDGRTVQIVHEK
                     ADIDLKVIHIHEEDAEAQINRFIRPFDLSTAPLIRAELLSISSARHLLLIDTHHIIADG
                     VSRSLFVKEIAQLYKGASLPEPKLHYKDYAVWQQQPDQQENIRKQEDFWLRQFKETVPE
                     LSLPLDFPRPPVQSFAGDRVHFKVSKGTALKIRRLMAKTNTTLNIVMLAVFNLFLSRLA
                     GQKDIVVGSAAAGRTNADLKDVPGMFVNSLALKNHVPDQASFSGFLEEVKNNSLAALDH
                     QDYPFEELIAKLDLPRDMSRNPLFNVMLTTEDPDKETLDLDGLIIKPYETSHAAAKFDL
                     TLGAFEKDDEIGLQFEYATDLFKKQTIERWSGYLLNLLEAIAENPDASLSDLSLLDEAD
                     KRRILYEWNETVLDVPQNKTVHELFEAQVLRTPDRGAAVYNG"
     aSDomain        1638..1721
                     /aSDomain="NRPS-COM_Nterm"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_B4090_RS05175_NRPS-COM_Nterm.1"
                     /evalue="2.00E-15"
                     /label="B4090_RS05175_NRPS-COM_Nterm.1"
                     /locus_tag="B4090_RS05175"
                     /protein_end="29"
                     /protein_start="1"
                     /score="47.7"
                     /tool="antismash"
                     /translation="GKEKIQKVYPLTPMQEGMLYHAMLDPES"
     aSDomain        1653..2552
                     /aSDomain="Condensation"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_B4090_RS05175_Condensation_DCL.1
                     "
                     /domain_subtypes="Condensation_DCL"
                     /evalue="9.70E-114"
                     /label="B4090_RS05175_Condensation_DCL.1"
                     /locus_tag="B4090_RS05175"
                     /protein_end="306"
                     /protein_start="6"
                     /score="371.4"
                     /specificity="C activity: active"
                     /specificity="Active site motif: HHIVMDG"
                     /tool="antismash"
                     /translation="QKVYPLTPMQEGMLYHAMLDPESSSYFTQLELVIDGEFDLGIFEK
                     SVNQLVRSYDILRTVFVHQQLQKPRQVVLAERQAIVEFEDLADLDEGRQKTRIDQYKQD
                     VQAAGFNLAKDMLFKTAVFRLDRKKHHLVWSNHHIVMDGWSMGILMKRLFQNYEAFRAN
                     RTVTLDQGKPYADYIKWLGRQDKDEAASYWEHRLSGLESPSVIPGGKTQPDSGSYRNEE
                     FTFVWDKDMVDALLKTANRYHVTAPNLFQAIWGAVLAKYNRTDDVVFGTVVSGRPSEID
                     GIEHMAGLFINTIPVRLKI"
     aSModule        1653..4739
                     /complete
                     /domains="nrpspksdomains_B4090_RS05175_Condensation_DCL.1"
                     /domains="nrpspksdomains_B4090_RS05175_AMP-binding.1"
                     /domains="nrpspksdomains_B4090_RS05175_PCP.1"
                     /locus_tags="B4090_RS05175"
                     /monomer_pairings="Val -> Val"
                     /tool="antismash"
                     /type="nrps"
     CDS_motif       1662..1700
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_B4090_RS05175_0001"
                     /evalue="1.10E-05"
                     /label="C1_dual_004-017"
                     /locus_tag="B4090_RS05175"
                     /protein_end="22"
                     /protein_start="9"
                     /score="18.0"
                     /tool="antismash"
                     /translation="YPLTPMQEGMLYH"
     CDS_motif       1725..1841
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_B4090_RS05175_0002"
                     /evalue="7.50E-14"
                     /label="C2_DCL_024-062"
                     /locus_tag="B4090_RS05175"
                     /protein_end="69"
                     /protein_start="30"
                     /score="44.0"
                     /tool="antismash"
                     /translation="SYFTQLELVIDGEFDLGIFEKSVNQLVRSYDILRTVFVH"
     CDS_motif       2058..2123
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_B4090_RS05175_0003"
                     /evalue="1.30E-12"
                     /label="C3_DCL_135-156"
                     /locus_tag="B4090_RS05175"
                     /protein_end="163"
                     /protein_start="141"
                     /score="40.0"
                     /tool="antismash"
                     /translation="NHHIVMDGWSMGILMKRLFQNY"
     CDS_motif       2169..2207
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_B4090_RS05175_0004"
                     /evalue="8.60E-03"
                     /label="C4_DCL_171-183"
                     /locus_tag="B4090_RS05175"
                     /protein_end="191"
                     /protein_start="178"
                     /score="9.1"
                     /tool="antismash"
                     /translation="PYADYIKWLGRQD"
     CDS_motif       2448..2543
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_B4090_RS05175_0005"
                     /evalue="5.70E-20"
                     /label="C5_DCL_263-294"
                     /locus_tag="B4090_RS05175"
                     /protein_end="303"
                     /protein_start="271"
                     /score="63.7"
                     /tool="antismash"
                     /translation="DVVFGTVVSGRPSEIDGIEHMAGLFINTIPVR"
     CDS_motif       2592..2708
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_B4090_RS05175_0006"
                     /evalue="7.20E-16"
                     /label="C67_DCL_14fromHMM"
                     /locus_tag="B4090_RS05175"
                     /protein_end="358"
                     /protein_start="319"
                     /score="50.6"
                     /tool="antismash"
                     /translation="QQHAIDAERYDYVPLYEIQKQSALAGRLISHLVAFENYP"
     aSDomain        3045..4220
                     /aSDomain="AMP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_B4090_RS05175_AMP-binding.1"
                     /evalue="1.80E-113"
                     /label="B4090_RS05175_AMP-binding.1"
                     /locus_tag="B4090_RS05175"
                     /protein_end="862"
                     /protein_start="470"
                     /score="371.2"
                     /specificity="substrate consensus: Val"
                     /tool="antismash"
                     /translation="FEEQVEASPDKTAVAADGRSLTYRRLNEKANQLARKLKAKGLMHG
                     NAAAILMERSLEAAVSMLAVLKAGGVYVPIDPEYPEERIRFLLEDSGAKIVLTKDSPQI
                     SLEGYEVLAANAVDAEKEDTANLVHANKPEDLAYYIYTSGSTGKPKGVMVEHRNIVRLV
                     KNAGYIPLKSDVKMAQTGAVSFDASTFEVFGALLNGGTLYPVPKETLLDGKRFRVFLEE
                     TGITTMWLTSPLFNQLAQQDPGMFATLSDLIIGGDALVPGIVNRVKRESPELSLWNGYG
                     PTENTTFSTCFFIDQAYERTIPIGKPIGNSTAYIVDEHGALQPIGVPGELCVGGDGVAR
                     GYLNQPELTDEKFVGDPFAEGKRMYRTGDLAKWLPDGNIEFLGRIDHQVKVR"
     CDS_motif       3231..3272
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_B4090_RS05175_0007"
                     /evalue="2.20E-05"
                     /label="NRPS-A_a2"
                     /locus_tag="B4090_RS05175"
                     /protein_end="546"
                     /protein_start="532"
                     /score="17.6"
                     /tool="antismash"
                     /translation="LAVLKAGGVYVPID"
     CDS_motif       3453..3512
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_B4090_RS05175_0008"
                     /evalue="1.80E-10"
                     /label="NRPS-A_a3"
                     /locus_tag="B4090_RS05175"
                     /protein_end="626"
                     /protein_start="606"
                     /score="32.6"
                     /tool="antismash"
                     /translation="AYYIYTSGSTGKPKGVMVEH"
     CDS_motif       3882..3905
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_B4090_RS05175_0009"
                     /evalue="8.90E-01"
                     /label="NRPS-A_a5"
                     /locus_tag="B4090_RS05175"
                     /protein_end="757"
                     /protein_start="749"
                     /score="3.3"
                     /tool="antismash"
                     /translation="YGPTENTT"
     CDS_motif       4014..4103
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_B4090_RS05175_0010"
                     /evalue="2.90E-18"
                     /label="NRPS-A_a6"
                     /locus_tag="B4090_RS05175"
                     /protein_end="823"
                     /protein_start="793"
                     /score="57.9"
                     /tool="antismash"
                     /translation="PIGVPGELCVGGDGVARGYLNQPELTDEKF"
     CDS_motif       4185..4250
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_B4090_RS05175_0011"
                     /evalue="6.40E-12"
                     /label="NRPS-A_a8"
                     /locus_tag="B4090_RS05175"
                     /protein_end="872"
                     /protein_start="850"
                     /score="37.4"
                     /tool="antismash"
                     /translation="FLGRIDHQVKVRGFRIELGEVE"
     aSDomain        4536..4739
                     /aSDomain="PCP"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_B4090_RS05175_PCP.1"
                     /evalue="2.30E-24"
                     /label="B4090_RS05175_PCP.1"
                     /locus_tag="B4090_RS05175"
                     /protein_end="1035"
                     /protein_start="967"
                     /score="77.3"
                     /tool="antismash"
                     /translation="EKILAAIWEEILGIKQPGIDDNFFSIGGHSLKAMMLTAKIQEQLQ
                     KEVPIKVLFEKPTIRELAEFLQG"
     aSDomain        4785..5645
                     /aSDomain="Condensation"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_B4090_RS05175_Condensation_LCL.1
                     "
                     /domain_subtypes="Condensation_LCL"
                     /evalue="1.90E-104"
                     /label="B4090_RS05175_Condensation_LCL.1"
                     /locus_tag="B4090_RS05175"
                     /protein_end="1337"
                     /protein_start="1050"
                     /score="340.8"
                     /specificity="C activity: active"
                     /specificity="Active site motif: HHIITDG"
                     /tool="antismash"
                     /translation="ANYPVSSAQRRMYILNQLEEASTSYNVPAVLLLEGELDKERLEDA
                     FRALISRHETLRTSFELIDGEIVQTIHQDAVFHLTESVADEAEAEAAIRGFIRPFQLNK
                     APLVRAELVKLEEKRHLLMIDMHHIITDGSSTGILIRDLASFYHGEEAPAPKLHYKDFA
                     VWQNGPEHQEKLKEQEQYWLDIFKGELPVLDLPYDFPRPSKRSFEGDRVVFGINRKLTA
                     DVQKLLSEADATLYMFLLAAFNILLSKYASQEDLIVGSPVAGRTHPDLHNIPGMFVNTL
                     ALRNRP"
     aSModule        4785..7877
                     /complete
                     /domains="nrpspksdomains_B4090_RS05175_Condensation_LCL.1"
                     /domains="nrpspksdomains_B4090_RS05175_AMP-binding.2"
                     /domains="nrpspksdomains_B4090_RS05175_PCP.2"
                     /locus_tags="B4090_RS05175"
                     /monomer_pairings="Asp -> Asp"
                     /tool="antismash"
                     /type="nrps"
     CDS_motif       4851..4964
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_B4090_RS05175_0012"
                     /evalue="3.00E-17"
                     /label="C2_LCL_024-062"
                     /locus_tag="B4090_RS05175"
                     /protein_end="1110"
                     /protein_start="1072"
                     /score="55.0"
                     /tool="antismash"
                     /translation="TSYNVPAVLLLEGELDKERLEDAFRALISRHETLRTSF"
     CDS_motif       5160..5225
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_B4090_RS05175_0013"
                     /evalue="2.30E-09"
                     /label="C3_LCL_132-143"
                     /locus_tag="B4090_RS05175"
                     /protein_end="1197"
                     /protein_start="1175"
                     /score="29.7"
                     /tool="antismash"
                     /translation="MHHIITDGSSTGILIRDLASFY"
     CDS_motif       5256..5285
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_B4090_RS05175_0014"
                     /evalue="9.70E-04"
                     /label="C4_LCL_164-176"
                     /locus_tag="B4090_RS05175"
                     /protein_end="1217"
                     /protein_start="1207"
                     /score="11.9"
                     /tool="antismash"
                     /translation="HYKDFAVWQN"
     CDS_motif       5547..5636
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_B4090_RS05175_0015"
                     /evalue="3.90E-15"
                     /label="C5_LCL_267-296"
                     /locus_tag="B4090_RS05175"
                     /protein_end="1334"
                     /protein_start="1304"
                     /score="48.2"
                     /tool="antismash"
                     /translation="DLIVGSPVAGRTHPDLHNIPGMFVNTLALR"
     CDS_motif       5685..5804
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_B4090_RS05175_0016"
                     /evalue="1.40E-13"
                     /label="C67_LCL_14fromHMM"
                     /locus_tag="B4090_RS05175"
                     /protein_end="1390"
                     /protein_start="1350"
                     /score="43.1"
                     /tool="antismash"
                     /translation="KETSLQGFAKQDYPLEELIEKLPLLRDTSRNPLFSVFFNM"
     aSDomain        6141..7349
                     /aSDomain="AMP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_B4090_RS05175_AMP-binding.2"
                     /evalue="1.30E-112"
                     /label="B4090_RS05175_AMP-binding.2"
                     /locus_tag="B4090_RS05175"
                     /protein_end="1905"
                     /protein_start="1502"
                     /score="368.4"
                     /specificity="substrate consensus: Asp"
                     /tool="antismash"
                     /translation="FEEKAAEMPDQPAVVCGETILTYRGLDERANQIANVLRSEGIGKD
                     DVVGIMLDRSAEVAAAILGVMKSGGAFLPIDPEMPTSRIQYMLEDSKARWLLTEHSHQA
                     ALADWYNGRLIDVRNDTLMASKKRPHLIHDGASLAYIIYTSGTTGQPKGVQLEHRNLAN
                     YVSWFISEARLSASDKTMLLSSYAFDLGYTSLFPVLLAGGELHIVPKETYTEPETFIHY
                     IGEQGITYIKLTPSLFHTVVQPQSFALAKGLQSLRLIVLGGEKINPKDVERFHSRYPDT
                     RFINHYGPTETTIGAIAKPVEPGMIRQFAERPTIGRPISGAGALVLDASRRLVPAGAAG
                     ELYITGSGLARGYVNNRELTAAKFIENPYTPGTFMYRTGDLVRRLPNGEIEFLGRTDDQ
                     VKIR"
     CDS_motif       6327..6368
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_B4090_RS05175_0017"
                     /evalue="5.80E-04"
                     /label="NRPS-A_a2"
                     /locus_tag="B4090_RS05175"
                     /protein_end="1578"
                     /protein_start="1564"
                     /score="13.3"
                     /tool="antismash"
                     /translation="LGVMKSGGAFLPID"
     CDS_motif       6555..6614
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_B4090_RS05175_0018"
                     /evalue="1.40E-09"
                     /label="NRPS-A_a3"
                     /locus_tag="B4090_RS05175"
                     /protein_end="1660"
                     /protein_start="1640"
                     /score="29.9"
                     /tool="antismash"
                     /translation="AYIIYTSGTTGQPKGVQLEH"
     CDS_motif       6999..7022
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_B4090_RS05175_0019"
                     /evalue="6.50E-02"
                     /label="NRPS-A_a5"
                     /locus_tag="B4090_RS05175"
                     /protein_end="1796"
                     /protein_start="1788"
                     /score="6.5"
                     /tool="antismash"
                     /translation="YGPTETTI"
     CDS_motif       7143..7232
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_B4090_RS05175_0020"
                     /evalue="1.30E-14"
                     /label="NRPS-A_a6"
                     /locus_tag="B4090_RS05175"
                     /protein_end="1866"
                     /protein_start="1836"
                     /score="46.2"
                     /tool="antismash"
                     /translation="PAGAAGELYITGSGLARGYVNNRELTAAKF"
     CDS_motif       7314..7379
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_B4090_RS05175_0021"
                     /evalue="1.60E-11"
                     /label="NRPS-A_a8"
                     /locus_tag="B4090_RS05175"
                     /protein_end="1915"
                     /protein_start="1893"
                     /score="36.1"
                     /tool="antismash"
                     /translation="FLGRTDDQVKIRGYRIELKEIE"
     aSDomain        7671..7877
                     /aSDomain="PCP"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_B4090_RS05175_PCP.2"
                     /evalue="2.30E-23"
                     /label="B4090_RS05175_PCP.2"
                     /locus_tag="B4090_RS05175"
                     /protein_end="2081"
                     /protein_start="2012"
                     /score="74.2"
                     /tool="antismash"
                     /translation="EKALCGIWEEVLGVRPVGLDDNFFDLGGHSLKGMMLTSEIQSKLG
                     KKVPLKTLFDCPTAGLLARSIEAD"
     aSDomain        7923..8780
                     /aSDomain="Condensation"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_B4090_RS05175_Condensation_LCL.2
                     "
                     /domain_subtypes="Condensation_LCL"
                     /evalue="1.60E-102"
                     /label="B4090_RS05175_Condensation_LCL.2"
                     /locus_tag="B4090_RS05175"
                     /protein_end="2382"
                     /protein_start="2096"
                     /score="334.5"
                     /specificity="C activity: active"
                     /specificity="Active site motif: HHIIADG"
                     /tool="antismash"
                     /translation="WYPVSSAQQRMYALHHIEKNGTGYNMPSVLMLEGVLDTDRLRRAF
                     ADLVNRHETLRTAFVEIDGRTVQIVHEKADIDLKVIHIHEEDAEAQINRFIRPFDLSTA
                     PLIRAELLSISSARHLLLIDTHHIIADGVSRSLFVKEIAQLYKGASLPEPKLHYKDYAV
                     WQQQPDQQENIRKQEDFWLRQFKETVPELSLPLDFPRPPVQSFAGDRVHFKVSKGTALK
                     IRRLMAKTNTTLNIVMLAVFNLFLSRLAGQKDIVVGSAAAGRTNADLKDVPGMFVNSLA
                     LKNHV"
     CDS_motif       7992..8102
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_B4090_RS05175_0022"
                     /evalue="6.40E-17"
                     /label="C2_LCL_024-062"
                     /locus_tag="B4090_RS05175"
                     /protein_end="2156"
                     /protein_start="2119"
                     /score="53.9"
                     /tool="antismash"
                     /translation="YNMPSVLMLEGVLDTDRLRRAFADLVNRHETLRTAFV"
     CDS_motif       8298..8360
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_B4090_RS05175_0023"
                     /evalue="5.30E-08"
                     /label="C3_LCL_132-143"
                     /locus_tag="B4090_RS05175"
                     /protein_end="2242"
                     /protein_start="2221"
                     /score="25.4"
                     /tool="antismash"
                     /translation="HHIIADGVSRSLFVKEIAQLY"
     CDS_motif       8391..8423
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_B4090_RS05175_0024"
                     /evalue="5.50E-04"
                     /label="C4_LCL_164-176"
                     /locus_tag="B4090_RS05175"
                     /protein_end="2263"
                     /protein_start="2252"
                     /score="12.7"
                     /tool="antismash"
                     /translation="HYKDYAVWQQQ"
     CDS_motif       8682..8768
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_B4090_RS05175_0025"
                     /evalue="3.00E-11"
                     /label="C5_LCL_267-296"
                     /locus_tag="B4090_RS05175"
                     /protein_end="2378"
                     /protein_start="2349"
                     /score="35.7"
                     /tool="antismash"
                     /translation="DIVVGSAAAGRTNADLKDVPGMFVNSLAL"
     CDS_motif       8823..8936
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_B4090_RS05175_0026"
                     /evalue="5.70E-17"
                     /label="C67_LCL_14fromHMM"
                     /locus_tag="B4090_RS05175"
                     /protein_end="2434"
                     /protein_start="2396"
                     /score="54.1"
                     /tool="antismash"
                     /translation="NNSLAALDHQDYPFEELIAKLDLPRDMSRNPLFNVMLT"
ORIGIN
        1 gttggcggac atttggcagg atgttctcgg catagaccga atcggagtca cggacaattt
       61 cttcgcactc ggcggggact cgatcaaagg aattcaaatg gcaagccgcc ttcagcagca
      121 cggttggaag ctggagatga aggacttgtt tcagcacccg acgatcgggg agctcagtgc
      181 ttatgttcaa gcggctgacg atcagccgat cgatcagaac cctgtcgaag gcgaagtcac
      241 actgacgccg atccagcgct ggttctttga gcgcaaattt acgaacgagc atcattggaa
      301 ccaatcagtt atgcttcacg ctttatcagg attcgacccg gaagcggcag gcaaggcttt
      361 aaccaagctg atcgaacacc atgatgcact tcgcatggtt tatcagcgaa atggagacgg
      421 gattgttcag tacaacagag gtctggaaga aacagcggtt cgtcctgaag tcatccgctt
      481 caatgaatcg ggtgctgaac ttgaagctgc cgtgttaaag gaatcaaacc ggattcaaag
      541 cagtatcaat ttaaccgaag gcccgctttt gaaagcggcg atctttgaga cggatcaagg
      601 cgatcatctt ctcatcgtca ttcatcatct cgttgtggac ggaatttcat ggagaatcct
      661 ccttgaggat ttcgccgccg ggtacgccca agctgaaaaa ggggagccga tcattcttca
      721 agaaaagacg cactcttttg ctgaatatgc ggctcgcctg aaagaatacg caaggtcaaa
      781 agctttcgcg aaagaaatcg gctattggca ggaagtcgag aaggcggaga cggcatcgct
      841 ccctaaagat gatgaagcag aggacaaacg gatgcaccat acgaaaacgg ctgagttttc
      901 tctctcgaaa gaagaaacag aacagttgat gacaaaagtt catgaagcat ataacacgga
      961 aatgaacgac attttgctta cggcgttggg cttggcgctg aaagagtgga ccgggcagga
     1021 agactttatc atctgcttgg agggacacgg ccgggaggac atcatggaag gcttgaatat
     1081 ttcccggacg gtcggctggt ttacaagcca gttcccggcg ctgattcaac tgcgccattc
     1141 cgaagatatc ggctatcaaa tcaaacagat caaagaagag ttgcgccaca ttccgaataa
     1201 aggaatcggc tatggcattt atcgctattt gaccgaagaa ggcaaaaagg cgcagccgat
     1261 caagcatgat atcagcttta actatttggg ccagtttgca gagatggcgg attcgggtct
     1321 gttcacgaga tcagctttgc cgtccggcga tccgctcagc ccggaaacgg aaaaaccgaa
     1381 tgcgctagac atcgtcggct atatcgaaaa cggcattttg acaatgtcaa ttgcctatca
     1441 ttcactcgaa tacaaagaaa gcaccgtcgc cgctgtcgct gaaagcttta aaacttattt
     1501 gctgcaattg atcgatcatt gcttagagcg ggatggaggc gaactcaccc cgagcgatct
     1561 tggagatgac gaactaacgc ttgaagaatt agataaactg atggaaattt tctaacaaaa
     1621 aaacgaggtg ccttatgggg aaagaaaaga ttcaaaaggt gtatcctttg acaccgatgc
     1681 aggaaggaat gctgtaccac gcaatgcttg atccggaatc ttcctcttac ttcacacagc
     1741 tggagcttgt catagacggg gaatttgatt tggggatctt tgaaaaaagc gtgaatcagc
     1801 tcgtgcgttc atacgatatt ctcagaacgg tttttgttca tcagcaattg caaaaaccgc
     1861 gccaggttgt cctggctgaa cggcaggcaa tagtggaatt tgaggatctt gcggacttgg
     1921 atgaaggaag gcaaaaaacc cgcattgacc agtataaaca agatgtacag gcggccgggt
     1981 ttaatttggc gaaagacatg ctttttaaaa cggcggtctt tcggcttgac cggaaaaagc
     2041 accatctcgt ctggagcaac catcatatcg tgatggacgg ctggagcatg gggatattga
     2101 tgaaacgcct ttttcaaaac tatgaagcgt ttcgagcaaa ccggacggtt acgcttgatc
     2161 agggaaaacc ttatgcggac tatattaaat ggctcggcag acaggataag gatgaagcgg
     2221 catcctactg ggaacaccgc ctttcgggtt tagaaagccc aagcgtcatt cccggcggaa
     2281 aaacgcagcc ggattcagga tcgtaccgga acgaggagtt cacgttcgtt tgggacaaag
     2341 acatggtgga tgctcttcta aagacggcaa accgctatca tgtcacagct cccaaccttt
     2401 ttcaggcgat ttggggagca gtgcttgcca aatataacag aacagatgat gtggtgttcg
     2461 gcacagtggt gtccgggcgc ccgtcggaaa ttgacggaat cgaacatatg gcagggctgt
     2521 tcatcaacac gatccccgtc cgtttaaaga tcgataaaaa cgcatcgttt gcagagctgt
     2581 ttaaacaagc acagcagcat gcaattgacg cggaacggta cgactatgtc ccgctatatg
     2641 agattcagaa gcaatcggca ttggccggcc gcttgatcag ccaccttgtc gcgtttgaga
     2701 actatccgct tgataaagag cttgaaaacg gaacgatcca tgagcggctc ggcttttcca
     2761 tcaaggctgc cggagctttt gaacaaacga attacgactt taacttgatc gtttatccgg
     2821 gagcggagtg gacgatcaaa ttgaaataca acggcgcggc ttttgacgcg gctttcattg
     2881 agcaggccgc cggacattta acgagactgg cagaagaggc gattgcaaat cccgaatcaa
     2941 aagcagtcag cgccagcatg ttatcaaaac aggagcaaca aacactgctc gcttttaatc
     3001 aaacgaaaac cggttatcca aaagaaaaga cgattccgga gctgtttgaa gaacaagttg
     3061 aagcctctcc tgacaaaacg gctgttgccg cagacggaag aagcctgact taccgcaggc
     3121 tgaatgagaa agccaatcag ctcgcgcgaa agctgaaggc aaaaggcctg atgcacggaa
     3181 atgctgccgc cattttgatg gagcggtctc ttgaagcagc cgtcagcatg ctggcagtgc
     3241 tgaaagcagg cggcgtgtac gtgccgatcg atcccgaata tcctgaagag cggattcgct
     3301 tcttgctcga agacagcgga gcaaagatcg tccttacaaa agacagcccg cagatttcac
     3361 tggaagggta tgaggtactc gccgcaaatg cggtggatgc agaaaaagaa gacaccgcca
     3421 accttgtaca tgccaacaaa cccgaagatc ttgcctatta catttataca tccggatcga
     3481 cgggcaagcc gaagggcgtg atggtcgaac accgcaatat cgtccgcctt gtcaaaaatg
     3541 cgggctacat cccgctgaag agcgacgtga agatggcgca gacgggggct gtcagctttg
     3601 acgccagtac atttgaagtg ttcggcgcac tgctaaacgg gggaaccctt tatcccgtcc
     3661 cgaaagagac gctccttgat ggaaagcggt tcagagtgtt cctggaagag accggaatca
     3721 caacaatgtg gctgacttcg cctcttttta atcagctcgc ccagcaggat ccgggaatgt
     3781 ttgcaaccct aagtgatctg attatcggcg gagatgccct tgttcctgga atcgtcaaca
     3841 gagttaaacg cgaatcgccg gaactctcgc tttggaatgg atacggacct actgaaaata
     3901 caacattttc gacgtgtttt ttcatcgatc aagcatatga gagaacgatc ccgattggaa
     3961 agccgatcgg aaattcaacc gcctatatcg ttgatgaaca cggcgcgctt cagccgatcg
     4021 gcgtgcccgg cgagctgtgt gtcggcggag acggggtggc ccgcggctac ttgaaccagc
     4081 ctgagctgac agatgaaaag tttgtcggcg atccttttgc cgaagggaag cggatgtacc
     4141 gcacgggcga cttggcaaaa tggcttcccg acgggaatat cgaatttctc gggcggatcg
     4201 atcatcaagt gaaggttcgc ggcttccgga tcgaattagg cgaggttgaa gcggctttgg
     4261 cccgccttga gggaatttca gaagcggctg ctgtcattcg ggaaaacaat accggagaaa
     4321 ccgagatttg cgcctactac actgcagttg gcgcgaggcc gcccgcacag ctgcggacag
     4381 agctgtcccg gtcgcttccg gagtatatga ttccggctca tttgatcgaa ctcgacagca
     4441 tgccgctgac agccaacgga aaggttgaca aacggcagct gccggctcct atcgctgaag
     4501 agactgacag attcgaagcg ccgaaaaatg aaacggagaa aattctggcg gcaatctggg
     4561 aagagattct cggcatcaag cagccgggaa tcgatgacaa cttcttttct atcggcggac
     4621 actcgctgaa ggcgatgatg ctgacagcca aaatacaaga gcagctccaa aaagaagtgc
     4681 cgatcaaagt attgtttgaa aaaccgacga tcagagagct ggccgaattt cttcaagggg
     4741 aaacgaaaga gaaaaccgct ccgattgcgc cggcgccgtc ccgtgcgaat tatccggtgt
     4801 catccgccca acggcgtatg tacatcctca atcagctcga agaagcaagc acgagctaca
     4861 atgtgccggc ggttctgctt ctggaaggcg aattggataa ggagcgtctg gaagacgcgt
     4921 ttcgggcctt gatcagccgc catgaaacat tgcggacatc gttcgaatta atcgatggag
     4981 aaatcgttca aaccatccat caagacgcag tttttcacct gacggaatca gtggcggatg
     5041 aagcggaagc cgaagcggcg atcagagggt ttatccggcc gtttcagctg aataaggcgc
     5101 cgctcgtccg ggcggaactc gtaaagcttg aagaaaagcg ccatctgctg atgatcgata
     5161 tgcaccacat tattacggac ggaagctcca ccggcatatt gattcgcgac ctggccagct
     5221 tctaccacgg tgaagaggcg cctgctccta agctccacta taaggatttc gctgtgtggc
     5281 aaaacgggcc ggagcatcag gagaaattga aagaacagga acagtattgg ctcgatattt
     5341 tcaaaggcga gctgcctgtt ctcgatctgc cgtacgattt tccgcggcct tcaaaacgca
     5401 gctttgaagg cgaccgtgtc gtctttggaa tcaatcgaaa actgacggca gacgttcaaa
     5461 agctgctttc tgaagccgat gcgacacttt atatgttcct gttggccgcg tttaatattt
     5521 tgctgtccaa gtatgcgtct caggaagatt taatcgtcgg atcgcctgtg gccggcagaa
     5581 cccatccgga tctgcacaac attccgggca tgtttgtcaa tacgctggcg ctccgcaatc
     5641 gtcccgaagg ggaaaaaacg tttaaggaat ttttgcagga agtcaaagag acaagtcttc
     5701 agggctttgc caagcaggac tatccgcttg aagaactgat tgaaaaactg ccattgctgc
     5761 gggatacaag ccggaatccg ctgttcagcg tctttttcaa catgcagaac atggaaatcc
     5821 cgacgctgaa actcggagat ttgcagattt cgtcttactc ggtgcgccat cgcacagcga
     5881 agtttgatct gtcgcttgag gccgtggagc atgatgggga aatcggtttg agctttgact
     5941 atgccgtatc cttatttaaa gaagaaacga tcaggcgctg gagcggccac ttcttaaatg
     6001 tgattaaaac aatctgcgaa aatcccggca ttaagctccg ggacatcgaa ctgctttcag
     6061 acaaagagcg cggacttctc ctggaagaag tgaggcgcag ccgggcagaa cgcctagagg
     6121 agcggccgtt tcatgtacgg tttgaagaaa aggcggccga aatgcctgat cagccggctg
     6181 ttgtctgcgg ggaaacaatc ctcacatacc gcgggctaga tgaaagagca aatcaaattg
     6241 caaatgtgct gcgaagtgaa ggcatcggaa aagacgacgt tgtcggcatt atgctggatc
     6301 gttctgcaga ggtcgccgcg gcaattctcg gcgtaatgaa atcagggggc gcttttttgc
     6361 cgattgatcc ggaaatgccg acgagcagga ttcaatatat gctggaagac agcaaagccc
     6421 gctggctctt aacagaacat tcccatcaag ctgctctcgc agattggtat aacgggagat
     6481 tgatagatgt gagaaacgac acgctgatgg caagcaaaaa acgaccgcat ctaatccatg
     6541 acggggcgag tctggcgtac atcatctata catccggaac gacgggtcag ccgaaaggcg
     6601 ttcagctgga acaccgcaat ctggcaaact atgtttcctg gtttatcagt gaggcgcgcc
     6661 tgtcagcttc ggataaaacg atgcttttgt cttcctatgc gttcgatctc ggctacacga
     6721 gccttttccc ggtgctgctc gccggaggag agctccatat cgtcccgaaa gaaacgtata
     6781 cagaaccgga gacatttatt cattatatcg gtgaacaagg gatcacatat atcaagctga
     6841 cgccgtcgct gttccatacg gtcgttcagc cgcaaagctt cgctttggca aaggggcttc
     6901 agtcattgag actgattgta ctagggggag aaaaaatcaa tccgaaagat gtcgaacggt
     6961 tccattcccg ctatccggac acccggttca tcaatcatta cgggccgacg gaaacgacga
     7021 tcggtgccat cgctaaaccg gtcgaaccgg gcatgatccg gcaattcgcc gagcgcccga
     7081 cgattggacg gccgatttcg ggcgccggag cactggtatt ggacgcttcc cgccggcttg
     7141 ttcccgcggg agcggcaggc gagctgtata ttacgggaag cgggcttgca agaggatatg
     7201 tgaacaaccg ggaacttacg gctgcgaagt ttatcgaaaa tccttacaca ccgggcacat
     7261 tcatgtaccg gacgggggat ctcgtccgcc gtcttccgaa tggggagatt gaatttttag
     7321 ggcggacgga tgatcaggtg aaaatccgcg gctacagaat cgaattgaaa gaaattgaag
     7381 aggccttcat tggacttgaa catatagaga gagcggtcgt tctttcattt acggctgact
     7441 cagggctaga cgaactgtgc gcttatattc aagcgaaacg acagctgccg gtttccgaac
     7501 tgagagagcg tctttctgag cggcttccgt cgtacatgat cccgtcatat ttcgtaacgg
     7561 tggataaaat gccgcttgcg gcgaacggaa aagtggaccg cagcgcgctg ccggagccgc
     7621 aatgtctaag tggtcaaagc gattcatata gagcgccgtc aaccgaattt gaaaaagccc
     7681 tctgcggcat ttgggaggag gtgcttggcg ttcggccggt cggacttgac gacaatttct
     7741 tcgatcttgg cggacattca ttaaaaggaa tgatgctgac atcggaaatc caatccaagc
     7801 tcggaaaaaa agtaccgtta aaaacgttgt tcgattgtcc gacagccggc ttgctcgccc
     7861 gcagtatcga agccgatgga caggcagaac aaagcgggat tcagcctgcc gaaaaacgcg
     7921 actggtatcc cgtttcatcc gctcagcaac ggatgtatgc cctccatcat attgaaaaga
     7981 acggaacggg ctacaacatg ccgtctgttc tcatgctgga aggcgtactt gatacggatc
     8041 gcttaagacg ggcattcgca gacttggtga accgccatga aacattgcgg accgcattcg
     8101 ttgaaatcga tggccgcacc gttcaaattg tgcatgaaaa agcggacatt gacttaaagg
     8161 ttattcatat tcatgaagag gacgcagaag cacagatcaa ccgctttatc agaccatttg
     8221 atctcagcac cgctccgctg atcagagccg agctgctctc catcagctct gcgcgccatc
     8281 tgcttttgat tgatacgcat catattattg cggacggcgt ttccagaagc cttttcgtca
     8341 aagaaattgc acagctttat aaaggcgctt cgctccctga accgaagctg cattataaag
     8401 attatgcggt atggcagcaa caacctgacc agcaagaaaa cattcggaaa caggaagact
     8461 tctggctccg gcagtttaag gaaaccgttc cggaactcag ccttccgctc gactttccgc
     8521 gtcctcctgt ccaaagcttt gccggggacc gggttcactt taaagtatca aaaggcaccg
     8581 cccttaaaat ccgccgtttg atggcgaaaa cgaacacgac gctgaatatt gtaatgctcg
     8641 ccgtttttaa tctgttttta agccgtcttg ccggccaaaa ggatattgtc gtcggctcag
     8701 cggcagcggg cagaacgaat gctgatttga aagacgtgcc gggcatgttc gtcaactcgt
     8761 tggcgctgaa aaaccatgta cctgatcaag catcattcag cgggtttttg gaagaggtga
     8821 aaaacaacag cctcgcagcc ctcgaccatc aggactatcc gtttgaagag ctgattgcca
     8881 agcttgattt gccgcgcgat atgagccgga atccgctgtt caatgtcatg ctgacaacgg
     8941 aagatcctga taaagaaacg ctcgatttag acggtctaat aatcaagccg tatgagactt
     9001 cacatgccgc tgccaagttc gacctgacac tgggggcttt tgaaaaagat gatgaaatcg
     9061 gtcttcagtt cgaatatgca acagatttat ttaaaaaaca aacgattgaa cgctggagcg
     9121 gatatttact taacctgctc gaagcgattg ccgaaaaccc ggatgcaagc ctttcagacc
     9181 tttcgcttct ggatgaagcc gataaacggc ggatcttgta cgaatggaac gaaacagttc
     9241 tcgacgtgcc gcagaacaaa acagtccatg agctgttcga agcgcaagtc ctccgcacgc
     9301 ctgaccgcgg ggcagcggtg tacaacggc
//
