LOCUS       NZ_BBXG01000016        10801 bp    DNA     linear   CON 08-AUG-2024
DEFINITION  Herbidospora cretacea strain NBRC 15474, whole genome shotgun
            sequence.
ACCESSION   NZ_BBXG01000016
VERSION     NZ_BBXG01000016.1
KEYWORDS    WGS; HIGH_QUALITY_DRAFT; RefSeq.
SOURCE      Herbidospora cretacea
  ORGANISM  Herbidospora cretacea
            Bacteria; Actinomycetota; Actinomycetes; Streptosporangiales;
            Streptosporangiaceae; Herbidospora.
REFERENCE   1
  AUTHORS   Komaki,H., Ichikawa,N., Oguchi,A., Hamada,M., Tamura,T. and
            Fujita,N.
  TITLE     Genome-based analysis of non-ribosomal peptide synthetase and type-I
            polyketide synthase gene clusters in all type strains of the genus
            Herbidospora
  JOURNAL   BMC Res Notes 8, 548 (2015)
   PUBMED   26452464
  REMARK    DOI:10.1186/s13104-015-1526-9 Publication Status: Online-Only
REFERENCE   2  (bases 1 to 189997)
  AUTHORS   Hosoyama,A., Oguchi,A., Ichikawa,N., Kimura,A., Ohshita,R. and
            Fujita,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAY-2015) Contact:Director-General Department Of
            Biotechnology National Institute of Technology and Evaluation,
            Biological Resource Center (NBRC); 2-49-10 Nishihara, Shibuya-ku,
            Tokyo 151-0066, Japan URL :http://www.nite.go.jp/nbrc/index.html
COMMENT     ##Genome-Assembly-Data-START##
            Finishing Goal                    :: Noncontiguous Finished
            Current Finishing Status          :: High-Quality Draft
            Assembly Method                   :: newbler v. 2.6
            Genome Coverage                   :: 71x
            Sequencing Technology             :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_001570605.1-RS_2024_08_08
            Annotation Date                   :: 08/08/2024 02:39:29
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,566
            CDSs (total)                      :: 7,487
            Genes (coding)                    :: 7,430
            CDSs (with protein)               :: 7,430
            Genes (RNA)                       :: 79
            rRNAs                             :: 4, 5 (5S, 16S)
            partial rRNAs                     :: 4, 5 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 10 of 57
            Pseudo Genes (incomplete)         :: 49 of 57
            Pseudo Genes (internal stop)      :: 3 of 57
            Pseudo Genes (multiple problems)  :: 4 of 57
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 12:10:14
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 96357
            Orig. end                         :: 107158
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            BBXG01000016.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10801
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="2"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10801
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10801
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:5801](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5801
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     misc_feature    complement(353..371)
                     /note="TFBS match to LexA_variant_3, Repressor of DNA
                     damage response, confidence: weak, score: 20.16"
                     /tool="antismash"
     gene            511..978
                     /locus_tag="AW270_RS27100"
     CDS             511..978
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /locus_tag="AW270_RS27100"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="VOC family protein"
                     /protein_id="WP_061298914.1"
                     /transl_table=11
                     /translation="MPMPAHPPRNPASPFASFAGHHVGIRVPDFEAAKSWYVEKLDFRV
                     LQEWPYGELKLAYLSPADDDDFHVEILAGPVPFPKEVLDDLEVSLRHGGYQHICVHVDS
                     VDETRAELVRRGVDVIGEPFEIEDISRRLAFFRDPWGNMIELSETLPGAGA"
     gene            complement(983..1699)
                     /locus_tag="AW270_RS27105"
     CDS             complement(983..1699)
                     /GO_function="GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:TIGR03083.1"
                     /locus_tag="AW270_RS27105"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="maleylpyruvate isomerase family
                     mycothiol-dependent enzyme"
                     /protein_id="WP_061298915.1"
                     /transl_table=11
                     /translation="MDHMTALIEQNARFGDLLHAADPSTEVPTCPGWTLKQLFRHVGRG
                     DRWSAQIISDRVDGGLDPREVRDGKPPADTEGALAWLHGGPAVLRDALAATGASTDVWT
                     FLGPRPAEWWVRRRLHEATVHRADAAFALGQPYDLAPDLAADGIEEWIDTVAASRPTLD
                     PGVTVHLHTTDVPGEWMIHGEEGRTVWEPGHGKGTLAVRGRAEDLFLALVNRREEGLEI
                     FGDENLWASWRERARF"
     gene            complement(1653..2231)
                     /locus_tag="AW270_RS27110"
     CDS             complement(1653..2231)
                     /EC_number="3.6.-.-"
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /locus_tag="AW270_RS27110"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="NUDIX hydrolase"
                     /protein_id="WP_245674869.1"
                     /transl_table=11
                     /translation="MGRGLSVAAVVASGGEFPAWYAALPVAVSAAGVLFTDGHDRVLLV
                     RTTYKDDWDIPGGVIEVDGGETPAAAARRELLEELGLDREVGDLLSIDVRPPQPDRRAF
                     VAFLFDGGVLTPGDVAGIGFPDGEIAEVRLVSLGEVARFTDAALARRIRSTLLHPGPGL
                     LFLSDGEPTGDDRGHGPHDRPDRAERPVR"
     gene            complement(2240..2608)
                     /locus_tag="AW270_RS27115"
     CDS             complement(2240..2608)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF07366"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020546924.1"
                     /locus_tag="AW270_RS27115"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="WP_061298916.1"
                     /sec_met_domain="PF07366 (E-value: 1.4e-06, bitscore: 21.6,
                     seeds: 29, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNLREIARRFAATWQTGWATHDVDLLLSLYAPGIVHRSMPFREPH
                     QGISALRDYLTWSFSEEVVRSVEFGEPLVDGDTAAIEFRVAAEEGDLAGCVFVRFDENG
                     LAIETRDYWHMSDNPERV"
     gene            2632..3336
                     /locus_tag="AW270_RS27120"
     CDS             2632..3336
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012890269.1"
                     /locus_tag="AW270_RS27120"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="DUF72 domain-containing protein"
                     /protein_id="WP_061298917.1"
                     /transl_table=11
                     /translation="MLVGTSGWQYADWRGVLYPPGVPQKRWLEHYGEEFRTVENNGSFY
                     RLPKHETFRQWHDRTPADFVMAVKASRYLTHVRRLRDPREPVDRLMRMAEGLGDKLGPI
                     LIQLPPNLPYEEDALGDCLKCFPESVALAVEPRHESWWNDDTRRTLETHEAALVWADRR
                     GRRQGPLWQTTTWAYLRFHEGTSRKNPPEYGDQAMRTWLDRLPDEGYVYFNNDPTGAAV
                     RNARRFTTLATK"
     gene            3342..3959
                     /locus_tag="AW270_RS27125"
     CDS             3342..3959
                     /EC_number="2.1.1.-"
                     /GO_function="GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /GO_function="GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019048519.1"
                     /locus_tag="AW270_RS27125"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="WP_061298918.1"
                     /transl_table=11
                     /translation="MGKLLGAAELEVSPVVANNAMNRGRRLASYSRELGLDLAGLLPGR
                     RWLDLCCGEGHALAEGAMLGADVTGVDLVDHFAVRPGRNLRLVVAGVPGWRPGERFDLV
                     TVVHGLHYVGDKLRVLADVAGWLNEGGRFVASFDVASVEVPSRRGLVATLRGQGFGYDA
                     RAKRIWRTGPAVIEFPYDYLGADDTAGPNYTGQPAVVSYYGS"
     misc_feature    3990..4000
                     /note="TFBS match to HypR, L-hydroxyproline utilization
                     repressor, confidence: weak, score: 18.59"
                     /tool="antismash"
     gene            3994..5004
                     /locus_tag="AW270_RS27130"
     CDS             3994..5004
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009083254.1"
                     /locus_tag="AW270_RS27130"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="Dyp-type peroxidase"
                     /protein_id="WP_061298919.1"
                     /transl_table=11
                     /translation="MSPATPSGVEDYRSGVQAVLGPLSPSAIFIVATIKPTGHQAVRDL
                     GEDLAGLLRTVGFRSLDPGLSCVLGFGSQLWDRLYPGLPKPQGLHPLKEIRGAHYAPST
                     PGDLLIHIRANRHDLCFELAQLIMNRLRDGVASADEVHGFRYFDARDLLGFVDGTENPT
                     GDLALESVLIGDEDPDHAGGSYVLVQKYVHDLSTWNRLNTEDQEKVIGRHKLSNVELDD
                     DVKPANAHNALTVIEEDGRELKILRDNMPFGNASQGEFGTYFIGYSRTSELLEHMLTNM
                     FIGDPPGNYDRILDFSTALTGSLYFVPSGAFLEDPPAAQIPTRMAIGDLKGVPQL"
     gene            5001..5801
                     /locus_tag="AW270_RS27135"
     CDS             5001..5801
                     /GO_component="GO:0140737 - encapsulin nanocompartment
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: Linocin_M18"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009083253.1"
                     /locus_tag="AW270_RS27135"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="family 1 encapsulin nanocompartment shell
                     protein"
                     /protein_id="WP_061298920.1"
                     /sec_met_domain="Linocin_M18 (E-value: 2.9e-93, bitscore:
                     305.2, seeds: 61, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNNLHRELAPISDAAWAQIEEEATRTFKRHIAGRRVVDVHGPGGT
                     ELSAVSTGHTTPVTSPSPGLQVRRREVSRLIELRRPFELDREAVDDVERGSNDSDWQPV
                     KDAAAALAIAEDRAVFEGFDAGHISGVIPLSSHRPLVLPSDVRAYPDAIAQALGILRMA
                     GVDGPYSVLLSADSYTGVAETADHGYPVLEHIQRLVSGDIVWAPAITGAVVLTTRGGDF
                     DLHLGQDVSIGYLSHTDATVTLYLQESFTFLPFTAEAAIHLTMP"
     gene            5892..6524
                     /locus_tag="AW270_RS27140"
     CDS             5892..6524
                     /GO_function="GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA]"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1008: response
                     regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /locus_tag="AW270_RS27140"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="response regulator transcription factor"
                     /protein_id="WP_061298921.1"
                     /transl_table=11
                     /translation="MRVFVVDEDRQTAADIEAALGKSGYSVTVGPDRTAVLGYDVVFLG
                     AGNDDGAEACREIRRTSDVPIIVLSPRDDGAERVRGLRAGADDYLVKPVSMEELEARME
                     AVLRRTRGGKTDVLQAGDIQIDAKRYLVWRDGRNVPLTPKEFALLRRLVQTPGEVVTKQ
                     TLLLENWKDLSRSAARTLDVHIAHLRAKLGEPNVVETVRGVGYRLVP"
     misc_feature    6333..6335
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            6642..7292
                     /locus_tag="AW270_RS27145"
     CDS             6642..7292
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="AW270_RS27145"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="SRPBCC family protein"
                     /protein_id="WP_061298922.1"
                     /transl_table=11
                     /translation="MRKKPFRLLSAPLLALTMVLSVMSSPARADADPLVALQWELARTT
                     YTLIEALNHPNPALGRAARSQSISVDIAAPRDVVFAIYSNFENHIGANPLLQRVVVHSD
                     TTAAGVRTINQTAIENTPLQPGLPATPINTHAQQRINAAGYFYEVDSWTLPNVITHQTI
                     TFEDLGAAGTRVTENLTFEASVLLIDYTVSQGVMAHQGIQSVFKSRIEGGFYS"
     misc_feature    6666..6668
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            complement(7289..8023)
                     /locus_tag="AW270_RS27150"
     CDS             complement(7289..8023)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1136: GntR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012893796.1"
                     /locus_tag="AW270_RS27150"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="GntR family transcriptional regulator"
                     /protein_id="WP_061298923.1"
                     /transl_table=11
                     /translation="MHPRYARIADELRAAILGGEIPVGTPLPSETVLAARHQVARGTVR
                     QAVAVLAAEGLVGSRQGARRIVLGRERSQNFAVLHSFAQWARAAGHEVTGQVIEQRRRT
                     ADAAEAARLGLAVGDPVLRVLRLRRLDGAAALLERTVYAGWLAPAVERLPADCDSVTQA
                     LYESRGLVFAYGEHLIDAVAAGSEDARLLGVRRGGPLLRQRRVTTTREGRPAEWSDDRY
                     RAGSVTFAIRNAAGALAVNPPG"
     misc_feature    complement(7643..7645)
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            8213..9388
                     /locus_tag="AW270_RS27155"
     CDS             8213..9388
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012893797.1"
                     /locus_tag="AW270_RS27155"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="WP_061298924.1"
                     /transl_table=11
                     /translation="MVSSRVRLTGLTAGLLIAVAACGSAPGTEATSTSSAAASGGAAAV
                     DARTATSAADFGGVDKLYEAAKAEGKLHVIALPPDWANYGAILAAFKAKYPGIEIEEET
                     PDASSADEINAVKTRKGQDRAPDVLDIGQSFAYSGAAEGLFAPYKVATWDKIPAAQKEA
                     DGLWVNDYGGYVSIACDAKKIAACPQTFADLLKPEYKGKVAMNGNPTKAGSAFAGVYAA
                     ALANGGSFDDIQPGIDFFKKLKDAGNFNPVETTPATIEKGETQISIDWDYNNAAYAPIM
                     AAKGLDWKTVIPTDGKYISLYAQAINKDAPHPAAARLWQEFLYSTEGQNLYLKGFARPV
                     LLPAMIADGSVDQAASAALPPVDGEPAFPTADQVTKANEVVASGWGAAVAG"
     gene            9388..10236
                     /locus_tag="AW270_RS27160"
     CDS             9388..10236
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1067: putative ABC
                     transporter permease protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020542070.1"
                     /locus_tag="AW270_RS27160"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="ABC transporter permease subunit"
                     /protein_id="WP_061298925.1"
                     /transl_table=11
                     /translation="MTGPVRSRRRGGGAGWLAAAPLLAVTLIAFGIPVVVLVVGAFTVK
                     TGGLGFDNLSATMREPYIGALWSSVKLSAVVAVAGAILGAFLAQAVLTSRSGLLREAVL
                     TASGVLSNFGGVPLAFIWIATLGNSGVVTAALGLGPGALYNFWGLALVYLYFSVPLMVL
                     VIAPALDGLKPQWREAAQNNGASTWQFWRMVGIPVLGPSLLGGGVLLFGGAFAAYATAA
                     AMVGASVPLVTLQIADALTGNVLIGYENIALALSLDMIVIALLVMAVYLPLQKRSSRWL
                     A"
ORIGIN
        1 cggcgggcac cgggtcgagg gcgtcgagca tcatccgcac cgccggccgg gcccggggcg
       61 gccggtcgtc tcccgggtcg gccaccggct tgaccaggtc gaacaggtgg cgccgctcca
      121 ggtcgccgag ccgcagggcg tcggcgaccg cgcccagcac ctgctcggag gcgtgggggg
      181 cgcggccctg ctccaggcgc gtgtagtagt cgacgctgac accggccagc atcgccagtt
      241 cctcccggcg caggcccgcc acgcggcggc tcccggccct gatcggcagc ccgacgtcgg
      301 ccggggtcag tcgggcccgg cgcgagcgca ggaattcgcg aatctccgct ggcctggaca
      361 tgggtccagt atcccgcgcc tagggtggtc ctgcgggaac caggttgagc ggtgccaggc
      421 tgggcgttca cccggcgggt cccggccggt tgacttcagt ggggccgccc gaggggggcg
      481 gcccgttctc tcttccgctg gagttgcacc atgccgatgc cggcccaccc gcccaggaac
      541 cccgccagcc cgttcgcgtc gttcgccggg caccacgtcg gaatccgggt cccggacttc
      601 gaggccgcca agtcctggta cgtcgagaag ctcgacttcc gggtcctgca ggaatggccg
      661 tacggggagc tgaagctggc ctatctgagc cccgccgacg acgacgactt ccacgtcgag
      721 atcctcgccg ggccggtccc gtttcccaag gaggtcctgg acgacctgga ggtgagcctg
      781 cgccacggcg gctaccagca catctgcgtc cacgtggaca gcgtcgacga gacccgggcc
      841 gaactggtcc ggcgcggcgt ggacgtgatc ggcgagccgt tcgagatcga ggacatcagc
      901 cgccgcctgg ccttcttccg cgacccgtgg ggcaacatga tcgagctctc cgagaccctg
      961 ccgggcgccg gggcctgaga ggtcagaacc gggcgcgttc ccgccacgac gcccagaggt
     1021 tctcgtcgcc gaagatctcc aggccctcct ccctccggtt gaccagggcc aggaagaggt
     1081 cctccgcgcg tccccggacc gcgagcgtgc ccttgccgtg gcccggctcc cagacggtcc
     1141 ggccctcctc gccgtggatc atccactcgc ccgggacgtc ggtcgtgtgg aggtgcacgg
     1201 tcacccccgg gtcgagggtg gggcgggagg ccgcgaccgt gtcgatccac tcctcgatgc
     1261 cgtcggcggc caggtcgggg gccaggtcgt agggctgccc cagggcgaag gcggcgtcgg
     1321 cgcggtggac ggtcgcctcg tggaggcggc ggcggaccca ccactccgcc gggcgggggc
     1381 cgaggaacgt ccacacgtcc gtcgacgctc cggtcgccgc gagcgcgtcc cgcaggacgg
     1441 ccgggccgcc gtgcagccag gccagggctc cttcggtgtc ggccgggggt ttgccgtcac
     1501 ggacctcgcg ggggtccagg ccgccgtcga cccggtcgga gatgatctgg gccgaccagc
     1561 ggtcgccccg gccgacgtgg cggaagagct gcttcagggt ccagccgggg caggtgggca
     1621 cctcggtgga ggggtcggcg gcgtggagga ggtcaccgaa ccgggcgttc tgctcgatca
     1681 gggcggtcat gtggtccatg gccacgatca tccccggtcg gctcgccgtc cgacaggaac
     1741 agcaggcccg gacccgggtg gagcagcgtg gacctgatcc ggcgggccag cgccgcgtcg
     1801 gtgaagcggg ccacctcgcc gagggagacc aggcggacct cggcgatctc gccgtcgggg
     1861 aacccgatcc cggccacgtc gccgggggtc agcacgccgc cgtcgaagag gaaggccacg
     1921 aacgcccgcc ggtccggctg cggggggcgg acgtcgatgc tcagcaggtc gcccacctcg
     1981 cggtcgaggc ccagctcctc cagcagctcc cgccgggccg ccgccgcggg ggtctcgccg
     2041 ccgtcgacct cgatcacgcc gccggggatg tcccagtcgt ctttgtacgt ggtccgcacc
     2101 agcagcaccc ggtcatgccc atcggtgaac agcactccgg ccgcggagac ggcgaccggg
     2161 agggcggcat accaggcggg aaattcaccg cccgatgcta caaccgccgc cacggacagg
     2221 ccccgaccca cacctggtcc taaacccgtt cgggattatc cgacatgtgc caataatcgc
     2281 gtgtctcgat ggcgagaccg ttttcgtcga agcgtacgaa aacgcatccc gccagatcgc
     2341 cctcctcggc ggcgacccgg aactcgatcg ccgccgtgtc gccgtcgacc agcggctccc
     2401 cgaactccac ggaccgcacg acctcctcgg agaacgacca cgtcagatag tcccgcaggg
     2461 ccgaaatgcc ctggtggggc tcgcggaacg gcatcgagcg gtggacgatg cccggggcgt
     2521 acagagaaag cagcaggtcg acgtcgtggg tggcccagcc ggtctgccag gtggcggcga
     2581 agcggcgggc gatctccctg aggttcaccc cgcgaagcct aggctggggg gatgttggtg
     2641 ggaacatccg gctggcagta cgccgactgg cgcggggtcc tctatccgcc gggtgtgccg
     2701 cagaaacgct ggctggaaca ctacggcgag gaattccgca ccgtcgagaa caacggctcc
     2761 ttctaccgcc tgccgaaaca cgagaccttt cggcaatggc acgaccggac gcccgccgat
     2821 ttcgtcatgg ccgtcaaggc gagccgatat ctcacccacg tcagacgcct gcgcgacccg
     2881 agggaaccgg tcgaccgcct gatgcgcatg gccgaaggcc tgggcgacaa actcggcccc
     2941 atcctgattc aactgccgcc caatctcccg tacgaggagg acgccctcgg agactgcctg
     3001 aaatgcttcc ccgaaagcgt cgccctggcg gtggaacccc gccacgagtc gtggtggaac
     3061 gacgacaccc ggcggaccct cgaaacccac gaggccgccc tcgtctgggc cgaccgccgg
     3121 ggccgccggc aggggccgct gtggcagacg accacctggg cctacctgcg cttccacgaa
     3181 ggaacgtcga ggaagaaccc gcccgaatac ggcgaccagg cgatgaggac ctggctcgac
     3241 cgcctgccgg acgagggcta cgtctacttc aacaacgacc ccaccggcgc ggcggtgcga
     3301 aacgcccgcc gcttcacgac attggcgaca aaataggccg catggggaag ctgctcgggg
     3361 ccgctgagct tgaggtgtct cccgtggtgg cgaacaacgc catgaaccgg gggcggcggc
     3421 tggcgagtta ctcgcgggag ctcgggctcg acctggccgg gctcctgcca ggcaggaggt
     3481 ggctggatct ctgctgcggg gaggggcatg ccctcgccga gggggcgatg ctcggggccg
     3541 acgtcacggg ggtcgatctg gtcgatcact tcgcggtgcg gccgggacgg aacctgcggc
     3601 tggtcgtggc gggcgtgccc gggtggcggc ccggcgagcg gttcgacctg gtcacggtcg
     3661 tgcacgggct gcactacgtc ggggacaagc tccgggtcct cgccgacgtc gccggatggc
     3721 tgaacgaggg cggcaggttc gtggccagct tcgacgtcgc gtcggtcgag gtgccgtccc
     3781 ggcgcgggct ggtggccact ctgaggggtc aggggttcgg gtacgacgcc cgcgccaagc
     3841 gcatctggcg gaccgggccc gccgtgatcg agtttcccta cgactacctg ggcgccgacg
     3901 acacggccgg gcccaactac accggccagc cggccgtcgt gtcgtactac gggtcataga
     3961 cgaatccgta ccggatcagg aggttcctca gccatgtcac cggcgacccc tagcggggtg
     4021 gaggactatc gcagcggcgt acaggcggtc ctgggtccac tgagtccctc ggccatcttc
     4081 atcgtcgcga cgatcaagcc gaccggccat caggcggtcc gggacctggg cgaagacctg
     4141 gcggggctgc tgcggacggt cgggttccgc tcgctcgacc cggggctgtc gtgtgtgctc
     4201 gggttcgggt cgcagctctg ggaccggctc taccccgggc tgccgaagcc gcaggggctc
     4261 catccgctca aggagatcag gggcgcccac tacgccccct ccacgccggg cgacctgctg
     4321 atccacatcc gggccaaccg gcacgacctc tgcttcgagc tggcccagct catcatgaac
     4381 cggctccggg acggggtcgc gagcgccgac gaggtgcacg gcttccgcta cttcgacgcc
     4441 cgtgacctgc tcggattcgt cgacggcacc gagaacccca cgggggacct ggccctcgaa
     4501 tcggtgctca tcggcgacga ggaccccgac cacgccggcg gctcctacgt cctggtccag
     4561 aaatacgtcc acgacctctc gacctggaac cggctgaaca ccgaggacca ggagaaggtc
     4621 atcggccgcc acaagctctc caacgtcgag ctcgacgacg acgtgaagcc cgccaacgcc
     4681 cacaacgccc tcacggtcat cgaggaggac ggcagggaac tgaagatcct gcgcgacaac
     4741 atgccgttcg gcaacgcctc gcagggcgag ttcggcacct acttcatcgg ctactcgcgc
     4801 acgtccgagc tgctggaaca catgctgacc aacatgttca tcggtgaccc gccgggcaac
     4861 tacgaccgga tcctcgactt ctccacggcg ctgaccggct cgctctactt cgtcccgtcg
     4921 ggcgccttcc tcgaagaccc tcccgcagcg cagatcccca ccaggatggc catcggcgac
     4981 ctcaagggag tcccgcagct atgaacaacc tccatcgcga gctcgccccg atctccgacg
     5041 ccgcctgggc gcagatcgag gaggaggcca cccggacgtt caagcgccac atcgccggcc
     5101 gccgggtggt cgacgtccac ggcccgggag ggaccgagct gtcggccgtg tcgaccgggc
     5161 acacgacgcc ggtcacctcg ccgtcgcccg gcctccaggt ccggcggcgg gaggtgtccc
     5221 ggctgatcga gctgcgccgg ccgttcgagc tggaccgcga ggcggtcgac gacgtcgagc
     5281 gcggctcgaa cgactccgac tggcagccgg tcaaggacgc cgccgccgcg ctggccatcg
     5341 ccgaggaccg ggccgtcttc gaggggttcg acgcggggca catctcgggc gtcatcccgc
     5401 tcagttccca ccggccgctg gtgctcccct ccgacgtccg cgcctatccg gacgcgatcg
     5461 cgcaggccct cggcatcctg cggatggccg gggtcgacgg gccctattcg gtgctcctca
     5521 gcgccgactc ctacacgggc gtcgccgaga cggccgacca cggatatccg gtgctggagc
     5581 acatccagcg cctcgtctcg ggcgacatcg tctgggcgcc ggcgatcacc ggcgcggtcg
     5641 tgctcaccac ccggggcggc gacttcgacc tgcatctcgg gcaggacgtc tccattggat
     5701 atctgagcca caccgacgcc acggtgacgc tctatctgca ggagtcgttc acgttcctgc
     5761 cgttcaccgc cgaggcggcc attcatttga ccatgcccta attgattctt cggcaattgt
     5821 tgacatgtca tcctgaaccc accagtattg cccggacgag gcgggggcca cagctttaaa
     5881 cacggggaga catgcgggta ttcgtcgtgg acgaagacag gcagacggcg gccgacatcg
     5941 aagccgcact ggggaaatca ggttattcgg tcacggtcgg acccgaccgg acggccgtgc
     6001 tcggatatga cgtcgtgttc ctgggggcgg gaaacgacga cggggccgag gcgtgccggg
     6061 agatccggcg gacgagcgac gtcccgatca tcgtgctgtc gccccgggac gacggagccg
     6121 aacgggtccg cggactacgg gccggcgccg acgactacct ggtcaaaccg gtctccatgg
     6181 aggaactgga agcgcgcatg gaggccgtgc tgcgccgcac gcggggcggc aagaccgacg
     6241 tcctccaggc gggcgacatc cagatcgacg ccaagcgcta tctggtctgg cgggacggcc
     6301 ggaacgtgcc actgacgccg aaggagttcg cgttactccg gcggctggtg cagacacccg
     6361 gcgaggtcgt caccaagcag accctgctgc tggagaactg gaaggacctc agccggtcgg
     6421 cggcgcgcac cctcgacgtg cacatcgccc atctgcgggc caagctcggc gagccgaacg
     6481 tcgtggagac cgtgcgggga gtggggtacc gattggtgcc ttgatattgg gcagattccg
     6541 ggcgcgcgag ccgcggtcgg ccttcggccg cccgcgtctc gcttaaagac tggcaggtct
     6601 tgccaggagc gttccaacgg cttctggggt gagatgtggc catgagaaag aagccgttcc
     6661 ggctgttaag cgctccactg ctcgccctca cgatggtcct gtccgtcatg agctcgcccg
     6721 ccagggccga cgccgacccg ctggtcgccc tccagtggga gctggcccgc acgacgtaca
     6781 ccctgatcga ggccctgaac catccgaacc ccgccctggg ccgggccgcg aggtcgcaga
     6841 gcatctccgt cgacatcgcg gccccccgcg acgtggtgtt cgcgatctac tcgaacttcg
     6901 agaaccacat cggcgccaac ccgctgctcc agcgggtggt cgtccactcc gacaccacgg
     6961 cagccggggt ccggaccatc aaccagaccg cgatcgagaa cacgccgctc cagccggggc
     7021 tgccggcgac gccgatcaac acccacgccc agcagcggat caacgcggcc gggtacttct
     7081 acgaggtcga ctcgtggacg ctgcccaacg tcatcaccca ccagacgatc accttcgagg
     7141 acctcggcgc ggccgggacc cgggtgaccg agaacctgac gttcgaggcc agcgtcctgc
     7201 tgatcgacta caccgtctcg cagggcgtca tggcccacca gggcatccag tcggtgttca
     7261 agagccgcat cgagggcggc ttctacagct agccgggcgg gttcaccgcc agggcgcccg
     7321 ccgcgttgcg gatcgcgaac gtcacgctgc cggcccggta gcggtcgtcc gaccactccg
     7381 ccgggcgccc ttcccgcgtc gtcgtcaccc gccgctgccg cagcagcggc ccgccccgcc
     7441 gcaccccgag caggcgggcg tcctcgctcc cggccgcgac ggcgtcgatc aggtgctctc
     7501 cgtacgcgaa caccagcccc cgggactcgt acagggcctg cgtgaccgag tcgcagtcgg
     7561 cgggcagccg ttcgactgcg ggggcgagcc agcccgcgta cacggtccgt tccagcaggg
     7621 ccgccgcgcc gtcgaggcgg cgtaaccgca gcacccgcag caccggatcg cccaccgcca
     7681 gcccgagccg ggccgcctcg gcggcgtcgg ccgtacggcg gcgctgctcg atgacctggc
     7741 cggtcacctc atgtccggcg gcccgcgccc actgcgcgaa actgtgcagc accgcgaagt
     7801 tctgactgcg ctcgcggccc agcacgatcc gcctggcccc ctgccgggag ccgaccagcc
     7861 cctcggccgc cagcaccgcc acggcctgac ggacggtccc ccgggcgacc tggtgccggg
     7921 cggcgagcac ggtctccgac ggcagcggcg tgcccaccgg gatctcgccg ccgaggatcg
     7981 ccgcccgtag ttcgtcggcg atgcgcgcgt aacgcgggtg cacggcggct ccccaccttc
     8041 gtgtgtacag ggcaggcttc accgtacggt ccggaaaacg tccggaaaag taggggttta
     8101 gccatttgac gaatggcctc cgactgttca ccggcaggac gtgggtggtc gatctggtgt
     8161 ccctagcttg tttggacaag ttctctgcac ttcgctccat ggggagttca ccgtggtctc
     8221 ttcccgagtc cgtctcaccg gcctgaccgc cggtctgctg atcgccgtcg cggcctgtgg
     8281 gtccgcgccc ggcaccgagg caacctccac gtcctccgcc gccgcctccg gtggcgccgc
     8341 cgcggtcgac gcccgcaccg ccacctcggc cgccgacttc ggtggcgtcg acaagctcta
     8401 cgaggccgcc aaggccgagg gcaagctgca cgtcatcgcc ctcccgcccg actgggccaa
     8461 ctacggcgcg atcctggccg ccttcaaggc caagtacccg ggcatcgaga tcgaggagga
     8521 gacccccgac gcctcctccg ccgacgagat caacgcggtg aagacccgca agggccagga
     8581 ccgcgccccc gacgtcctgg acatcggcca gtcgttcgcc tactccggcg cggccgaggg
     8641 cctgttcgcg ccgtacaagg tcgcgacctg ggacaaaatc cccgccgcgc agaaggaggc
     8701 cgacggcctc tgggtgaacg actacggcgg ctacgtctcc atcgcctgtg acgccaagaa
     8761 gatcgcggcc tgcccgcaga ccttcgccga cctgctcaag cccgagtaca agggcaaggt
     8821 cgcgatgaac ggcaacccga ccaaggccgg ttcggccttc gccggcgtgt acgccgccgc
     8881 gctcgccaac ggcggttcgt tcgacgacat ccagcccggc atcgacttct tcaagaagct
     8941 caaggacgcc ggcaacttca acccggtcga gacgaccccg gcgacgatcg agaagggcga
     9001 gacccagatc tcgatcgact gggactacaa caacgccgcc tacgcgccga tcatggccgc
     9061 caagggcctc gactggaaga ccgtcatccc caccgacggc aagtacatct cgctgtacgc
     9121 ccaggccatc aacaaggacg ccccccaccc ggccgccgcg cggctgtggc aggagttcct
     9181 ctacagcacc gagggccaga acctctacct gaagggcttc gcccgcccgg tgctgctgcc
     9241 cgcgatgatc gccgacggct cggtcgacca ggccgcctcc gccgccctgc cgccggtcga
     9301 cggcgagccc gccttcccca ccgccgacca ggtcacgaag gcgaacgaag tcgtcgcctc
     9361 cggctggggc gccgcggtcg ccggctgatg accggcccgg tacgttcccg gcgtcgcggt
     9421 ggcggcgccg ggtggctggc ggcggcgccc ctgctcgccg tcaccctgat cgccttcggc
     9481 atccccgtgg tggtgctggt cgtcggcgcg ttcaccgtca agaccggcgg gctcgggttc
     9541 gacaacctgt cggcgacgat gcgcgagccg tacatcgggg cgctgtggag cagcgtcaag
     9601 ctgtcggccg tcgtcgccgt cgcgggcgcg atcctcgggg cgttcctcgc gcaggcggtg
     9661 ctgacctcgc ggtcggggct gctgcgcgag gccgtgctga ccgcctccgg ggtgctgtcc
     9721 aacttcggcg gggtgccgct ggccttcatc tggatcgcca cgctcggcaa ctccggcgtg
     9781 gtcaccgccg ccctcggcct cggcccgggc gcgctctaca acttctgggg cctggctctg
     9841 gtctacctct acttctccgt gccgctgatg gtgctggtca tcgcccccgc gctcgacggc
     9901 ctcaagccgc agtggcgcga ggccgcgcag aacaacggcg cgtccacctg gcagttctgg
     9961 cggatggtcg gcatcccggt gctcggcccg tcgctgctcg gcgggggcgt gctgctgttc
    10021 ggcggggcgt tcgccgccta cgcgaccgcc gccgcgatgg tcggcgcgtc ggtgccgctg
    10081 gtgacgctcc agatcgccga cgccctcacg ggcaacgtgc tgatcgggta cgagaacatc
    10141 gccctcgcgc tgtccctcga catgatcgtg atcgcgctgc tggtgatggc cgtctacctg
    10201 cccctgcaga agaggagctc ccgatggctg gcgtgacctg gcgccgggtc accctcggcg
    10261 tggcggcggt ctacttcctg gtgcccctgg ccatcgcggt ctggttcacc gtctacaacg
    10321 agtcgcgggg cttctccctc ggcgcctacg gccggatcgt ctcggccccc ggcttcggtg
    10381 agagcctgct cacgtcgctg ctgctcgggg ccgcggtcat cgtgctggtc ctgctcctgg
    10441 cgctgcccgc tcttctggcg gtacgtctgg gggctccggg cctgcgcccg gtgctggaga
    10501 ccatcacgac gctgccgctg atcgtgccgc cgatcaccta cgtggtcggc atcgccaacg
    10561 cgctgcgcgg cggcaccgac gccctggcgg ccacgccgtt ctggcagacc ctcatctcca
    10621 tccaggacga ggacttcccc gtcgtcctgg tcctggcgta cgtcatgctc gcgctgccgt
    10681 tcgcctaccg ttcgctggac accggcctgc gggccatcga cgtgcggacc ctcgtggagg
    10741 cggcccggaa cctgggcgcc tcgtggccgt acgtgctgtt ccgggtgatc ctgccgaacc
    10801 t
//
