LOCUS       NZ_BBXG01000015        10810 bp    DNA     linear   CON 08-AUG-2024
DEFINITION  Herbidospora cretacea strain NBRC 15474, whole genome shotgun
            sequence.
ACCESSION   NZ_BBXG01000015
VERSION     NZ_BBXG01000015.1
KEYWORDS    WGS; HIGH_QUALITY_DRAFT; RefSeq.
SOURCE      Herbidospora cretacea
  ORGANISM  Herbidospora cretacea
            Bacteria; Actinomycetota; Actinomycetes; Streptosporangiales;
            Streptosporangiaceae; Herbidospora.
REFERENCE   1
  AUTHORS   Komaki,H., Ichikawa,N., Oguchi,A., Hamada,M., Tamura,T. and
            Fujita,N.
  TITLE     Genome-based analysis of non-ribosomal peptide synthetase and type-I
            polyketide synthase gene clusters in all type strains of the genus
            Herbidospora
  JOURNAL   BMC Res Notes 8, 548 (2015)
   PUBMED   26452464
  REMARK    DOI:10.1186/s13104-015-1526-9 Publication Status: Online-Only
REFERENCE   2  (bases 1 to 197492)
  AUTHORS   Hosoyama,A., Oguchi,A., Ichikawa,N., Kimura,A., Ohshita,R. and
            Fujita,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAY-2015) Contact:Director-General Department Of
            Biotechnology National Institute of Technology and Evaluation,
            Biological Resource Center (NBRC); 2-49-10 Nishihara, Shibuya-ku,
            Tokyo 151-0066, Japan URL :http://www.nite.go.jp/nbrc/index.html
COMMENT     ##Genome-Assembly-Data-START##
            Finishing Goal                    :: Noncontiguous Finished
            Current Finishing Status          :: High-Quality Draft
            Assembly Method                   :: newbler v. 2.6
            Genome Coverage                   :: 71x
            Sequencing Technology             :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_001570605.1-RS_2024_08_08
            Annotation Date                   :: 08/08/2024 02:39:29
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,566
            CDSs (total)                      :: 7,487
            Genes (coding)                    :: 7,430
            CDSs (with protein)               :: 7,430
            Genes (RNA)                       :: 79
            rRNAs                             :: 4, 5 (5S, 16S)
            partial rRNAs                     :: 4, 5 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 10 of 57
            Pseudo Genes (incomplete)         :: 49 of 57
            Pseudo Genes (internal stop)      :: 3 of 57
            Pseudo Genes (multiple problems)  :: 4 of 57
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 12:10:14
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 23359
            Orig. end                         :: 34169
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            BBXG01000015.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10810
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="1"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10810
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10810
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:5810](-)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5810)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            1127..1657
                     /locus_tag="AW270_RS25825"
     CDS             1127..1657
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="AW270_RS25825"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_061298470.1"
                     /transl_table=11
                     /translation="MRKRLSVLLLAALTGCGVTSTGVQDGGPPPVISPKAAVATVYLLR
                     AGKLEPSSVAVASHSMDNVMAALFEAGTRSTSGLTSELTGLVWQTSQLTRFGAVRNDPD
                     NADGFRLSLFVSGARPITKKAQAQMTCTAMRQRQDIWAVTITRTDVTGPVSYGEHTCRE
                     FWSLAAPGRQLPR"
     gene            complement(1654..2967)
                     /locus_tag="AW270_RS25830"
     CDS             complement(1654..2967)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012894895.1"
                     /locus_tag="AW270_RS25830"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="PhoH family protein"
                     /protein_id="WP_061298472.1"
                     /transl_table=11
                     /translation="MSGHTPGTSRRTYILDTSVLLADPAALSRFDEHEVVLPVVVITEL
                     EGKRHHPELGYFARKALRFLDDLRVQHGRLDEPIPMGEGTIRVELNHSDPSGLPAGFRL
                     GDNDTRILTVAQNLASEGLDVVLVSKDLPMRIKAASIGLTAEEYRAELVAESDETWTGM
                     AELQVTAEDVGELFEHGGTELEEARDLPTHTGLRLLTSKGSALGRVLPDKSVRLVRGDR
                     EVFGLHGRSAEQRIALDLLMDPEIGIVSLGGRAGTGKSALALCAGLEAVLERRQHRKVI
                     VFRPLYAVGGQELGYLPGSENEKMGPWAQAVYDTLGAVTTPEVIEEVLDRGMLEVLPLT
                     HIRGRSLHDAFVIVDEAQSLERGVLLTVLSRIGANSRVVLTHDVAQRDNLRVGRHDGVV
                     AVVEKLKGHPLFAHVTLTRSERSPIAALVTEMLEDITP"
     gene            complement(3222..3983)
                     /locus_tag="AW270_RS25835"
     CDS             complement(3222..3983)
                     /GO_function="GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017598077.1"
                     /locus_tag="AW270_RS25835"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="isoprenyl transferase"
                     /protein_id="WP_061298474.1"
                     /transl_table=11
                     /translation="MDLRDLVYKVYERRLERKLTSIQAPRHVGVIVDGNRRWARSNGHE
                     DVRHGHQKGADKISELLVWCKEAGVEYVTLWLLSTDNLSRDKKELEPLLQVIENLVTDL
                     VDQGWHVKPMGSLDLLPDHTAHVLKNAKEATSHRPGLIVNVAVGYGGRREIADAVRSLL
                     QEHASKGASIEDLVEVLDVEHIAEHLYTRGLPDPDLVIRTSGEQRLSGFLLWQSAHSEF
                     YFCEAYWPDFRRVDFLRALRAYAARNRRYGF"
     gene            complement(4039..4548)
                     /locus_tag="AW270_RS25840"
     CDS             complement(4039..4548)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012851444.1"
                     /locus_tag="AW270_RS25840"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_245674851.1"
                     /transl_table=11
                     /translation="MACRDSAGVPYEVTLELFRDGVPYGSVGERCGWYLARLARSVAEA
                     RDSQGAWDDPDDRFPGDDEEELFGFRYRSRTDVVGGGELRCQLRLIPVWVPGRGVTGRW
                     RLTRRAHVEAWGASGAGLRATLTSGELLIFLSTLVTESEVRLGTQYGMERNSSLISGAG
                     TSSRPR"
     gene            complement(4637..5008)
                     /locus_tag="AW270_RS40680"
     CDS             complement(4637..5008)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="AW270_RS40680"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_061298475.1"
                     /transl_table=11
                     /translation="MADPRAQLADQQRALLAALVAGAAPPHGFDAERLRIQASSLIAKR
                     RGLVAALRPDVVRALGQAFGREFHAYASGRPKPQGGSRADAAAFAEWLGERGLLAAAEP
                     ERQQKRHWWRITPKKDQYR"
     gene            complement(5001..5810)
                     /locus_tag="AW270_RS40685"
     CDS             complement(5001..5810)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: DUF692"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020545141.1"
                     /locus_tag="AW270_RS40685"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="DUF692 domain-containing protein"
                     /protein_id="WP_061298757.1"
                     /sec_met_domain="DUF692 (E-value: 7.4e-78, bitscore: 255.0,
                     seeds: 7, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MAADRLGVGIGWRSEIDLTVEHLPGVEFTEVIAETIVPARVPESL
                     RLMRGRGIPVIPHGVALGIGGAERPDQARLDHLAAVAQALGSPLVSEHLAYVRSGDLEA
                     GHLLPVPRTRDALSVIVENVRIALDCLPVPLALENVAALFGWPGDELTEAQFLGELVER
                     TGVKLLIDVANLYTNQVNLGLDAVKALDALPLEHVAYVHVAGGHGHGDVWHDTHTASPP
                     QEVLDLLAELSARHRPPGALLEWDADYPSDAVLSEELDRIRAALNRG"
     gene            complement(5800..6765)
                     /locus_tag="AW270_RS41430"
     CDS             complement(5800..6765)
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:TIGR04222.1"
                     /locus_tag="AW270_RS41430"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="TIGR04222 domain-containing membrane protein"
                     /protein_id="WP_061298477.1"
                     /transl_table=11
                     /translation="MDSLVLFIPAIVAVVLVFRQARRVKQEQRRVKSGGGYAPPGLDPY
                     SLAFLAGGPRRVVNTAVAVLVSQGRVRISRDGSVTAVRGIAGAPAERVEQEVLDLATGV
                     NGRPLADIRRTVCVGPAVTGIDYGLRGHGLVVADSALTSARRANTKLRWLCGLAGLFVP
                     VSIVSLAFGWFESVLWSVLTLIVSVLTWIIGMAVASGNAKENLSVLTPAGHAALRAAKA
                     RYPRTRELAYAHNSGNDSTIALYGLAIYTGPGHAEIQHGGGGCGAGACGGAVGADSGWG
                     SGSDFGGGDFGGGGSDSGGGGDSGGGSSCGGGCGGGCGGS"
     gene            complement(6984..8777)
                     /locus_tag="AW270_RS25860"
     CDS             complement(6984..8777)
                     /EC_number="4.1.1.32"
                     /GO_function="GO:0004611 - phosphoenolpyruvate
                     carboxykinase activity [Evidence IEA]"
                     /GO_function="GO:0005525 - GTP binding [Evidence IEA]"
                     /GO_function="GO:0017076 - purine nucleotide binding
                     [Evidence IEA]"
                     /GO_process="GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013133322.1"
                     /locus_tag="AW270_RS25860"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="phosphoenolpyruvate carboxykinase (GTP)"
                     /protein_id="WP_174549891.1"
                     /transl_table=11
                     /translation="MSVSLQLSHPTTHAELAAWVSEIAELTEPDRIEWCDGSEAEWTRL
                     TNMLVKQGTLTRLRARPNSFHAASDPSDVARVEDRTFICSEREKDAGPTNNWIEPGEMR
                     RTFGRLFKGSMRGRTMYVVPFCMGPLGGPISQLGVEITDSAYVAVSMKVMTRMGEEALR
                     LIEERGDYVKCVHSVGAPLAPGQEDVAWPCSDTKYISHFPETREIWSYGSGYGGNALLG
                     KKCYALRIASTMARDEGWLAEHMLILKLTPPSGETRYIAAAFPSACGKTNLAMLKPSIP
                     GWKVETIGDDIAWMRFGDDGRLYAINPEAGFFGVAPGTGETTNANAITTLWGNSIFTNV
                     ALTDDGDVWWEGLTDEPPAHLTDWRGHSWTPGSGELAAHPNARFTTPAHQCPTIAPEWQ
                     DPNGVPISAILFGGRRATAVPLVTEARSWRHGVFMGANVASEKTAAAEGKVGELRRDPF
                     AMLPFCGYNMGDYFAHWLDVGETEGAKLPRIYYVNWFRKDGEGRFVWPGFGENIRVLKW
                     IVERLNGQAEAVDTPIGRLPVKIDTEGLDIAEDDMDLLLTVDREVWREEAALIPAHFET
                     FGDHLPEGLWEEYRSLVARLG"
     misc_feature    8813..8827
                     /note="TFBS match to MexT, Global virulence regulator,
                     confidence: weak, score: 17.51"
                     /tool="antismash"
     misc_feature    8843..8858
                     /note="TFBS match to DasR, N-acetylglucosamine dependent
                     repressor, confidence: medium, score: 22.86"
                     /tool="antismash"
     misc_feature    8974..8989
                     /note="TFBS match to DasR, N-acetylglucosamine dependent
                     repressor, confidence: weak, score: 20.6"
                     /tool="antismash"
     misc_feature    complement(8993..9008)
                     /note="TFBS match to DasR, N-acetylglucosamine dependent
                     repressor, confidence: medium, score: 23.82"
                     /tool="antismash"
     misc_feature    9015..9030
                     /note="TFBS match to DasR, N-acetylglucosamine dependent
                     repressor, confidence: weak, score: 19.91"
                     /tool="antismash"
     gene            complement(9021..9530)
                     /locus_tag="AW270_RS25865"
     CDS             complement(9021..9530)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020545144.1"
                     /locus_tag="AW270_RS25865"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_061298479.1"
                     /transl_table=11
                     /translation="MSAESEWFAHRLPENWFTERPEVVVDREEITVVGALPPLTVTEGL
                     PDAERAAAEEGYVERFREETRERRIEIARQAEHRFRRKVAWGVKIGEQTVMFTTLSVPV
                     MTRLRQGERQVLDTLVAAGVARSRSDALAWCVRLVGRHTDTWLADLRDALQAVDRVRAA
                     GPGGVD"
     misc_feature    9581..9596
                     /note="TFBS match to ColR, Phenol-responsive regulator,
                     confidence: weak, score: 17.99"
                     /tool="antismash"
     misc_feature    9586..9595
                     /note="TFBS match to ToxT, Regulator of MSHA biosynthesis,
                     confidence: weak, score: 15.37"
                     /tool="antismash"
     misc_feature    9591..9606
                     /note="TFBS match to FuR_variant_1, Ferric Uptake
                     Regulator, confidence: weak, score: 18.42"
                     /tool="antismash"
     misc_feature    complement(9591..9609)
                     /note="TFBS match to FuR_variant_2, Ferric Uptake
                     Regulator, confidence: weak, score: 23.23"
                     /tool="antismash"
     misc_feature    9594..9607
                     /note="TFBS match to PerR, Peroxide regulator, confidence:
                     weak, score: 15.74"
                     /tool="antismash"
     misc_feature    complement(9594..9609)
                     /note="TFBS match to FuR_variant_1, Ferric Uptake
                     Regulator, confidence: weak, score: 19.43"
                     /tool="antismash"
     misc_feature    complement(9663..9676)
                     /note="TFBS match to ANR, Anaerobic transcriptional
                     regulator, confidence: weak, score: 19.32"
                     /tool="antismash"
ORIGIN
        1 cgccccggtt ctctgaaggc gcgcacgtca ccctggccga catccccgac gacgtccggg
       61 ccgccgcccg cacccggatc gcctaccgga cgatggaggt cgaccaggag ccgacgctcg
      121 tggtcggcac cgacgtcaaa cgccagaccg gcagcggcgc ggtccgctcc accggcatga
      181 cggtcttcgt gttcgtctcc atgaccgacg tcgaccgggt gctggtcaac ctgcgcgccg
      241 cgctgctcca ggcgggctcg ctcacgctcg tcctcgcgct cacggtggcg ctgctgtcgg
      301 cccgccaggt ccttcttccg gtacgccgcc tcggtgaggc cgcccgcgcg ctgggagccg
      361 gccggctcga cacccggctg ccggtccacg gccgcgacga actcgccgag ctcaccgcca
      421 cgttcaacga gacggcgcgg gcgctggagc agaccgtcca ggagctgtcg tcgctggagg
      481 cgatgtcgcg gcgcttcgtc gcggacgtct cccacgagct gcgcaccccg ctgaccacca
      541 tgacggcggt caccgacatg ctctccgacg aggccgaacg gctgccgccg gacgccgggc
      601 aggccgtcca cctcgtggtc gccgagatcg agcggctgcg ctccctggtc gacgacctga
      661 tcgaggtcag ccgcttcgac tccggcgcgg ccgtgctccg ccgcgagacc gtcgacgtcg
      721 gcgacgagct gaccgagtgc ctggagatcc gcggctggac cgaccaggtg accctgcggg
      781 tccccgacgg cctgacctac cgggtcgacc cgcgccgctt cgacgtcatc gtcgccaacc
      841 tcgtcggcaa cgccctgaag cacggcgaac gccccgtcac cgtcaccgcc aaggtcctgc
      901 ccagcggcct ggaggtccgc gtccgcgacc acggccccgg catcccccgc gaggacctgc
      961 cccacgtctt cgagcgcttc ttcaagaccg gcgccaaccg ggcccgctcg ggcggcagcg
     1021 gcctgggcct gtcgatcgcc aaggccaacg ccgaactcca ccgcggcacg atcaccgtca
     1081 aactcgccga ccccggaacc cagttcacgt tgtggatacc gcgcccatga ggaaacggct
     1141 cagcgttctg ctcctggcgg ccctgaccgg ctgtggggtc acctcgaccg gcgtccagga
     1201 cggcggcccg ccgccggtga taagccccaa ggccgccgtc gcgaccgtct acctgctgcg
     1261 cgcgggcaag ctggagccca gcagcgtcgc ggtggcctcc cactcgatgg acaacgtgat
     1321 ggccgccctc ttcgaggcgg gcacccgctc gacctcgggc ctgaccagcg agctcaccgg
     1381 cctggtctgg cagaccagcc agctcacccg gttcggggcg gtccgcaacg accccgacaa
     1441 cgccgacggc ttccggctgt cgctgttcgt cagcggcgca cgcccgatca ccaaaaaggc
     1501 gcaggcccag atgacctgca ccgccatgcg gcagcggcag gacatctggg ccgtcacgat
     1561 caccaggacg gacgtcaccg gcccggtgtc ctacggtgag cacacctgcc gcgagttctg
     1621 gagtctcgcg gcgccgggcc ggcaactgcc ccgttagggc gtgatgtctt ccagcatctc
     1681 ggtcaccagg gccgcgatcg gggagcgttc cgaacgcgtc agggtgacgt gggcgaacag
     1741 cgggtggccc ttgagcttct cgaccaccgc gaccacgccg tcgtgccggc cgacccgcag
     1801 gttgtcgcgc tgggcgacgt cgtgggtcag cacgacccgc gagttggcgc cgatgcgcga
     1861 caggaccgtc agcagcacgc cgcgttccag cgactgcgcc tcgtcgacga tcacgaacgc
     1921 gtcgtggagc gaccggccgc ggatgtgggt cagtggcagg acttccagca tgccccggtc
     1981 gaggacctcc tcgatcacct cgggcgtcgt caccgcgccc agggtgtcgt agacggcctg
     2041 ggcccacggg cccatcttct cgttctcgct gccgggcagg tagccgagct cctggccgcc
     2101 gaccgcgtag agcgggcgga acacgatgac cttgcggtgc tggcggcgtt cgagcacggc
     2161 ctcgaggccc gcgcagagcg ccagcgccga cttgccggtg ccggcccggc cgcccagcga
     2221 gacgatcccg atctccgggt ccatcagcag gtcgagcgcg atgcgctgct cggcgctgcg
     2281 gccgtggagg ccgaagacct cgcggtcgcc gcgcaccagg cgcaccgact tgtccgggag
     2341 cacccggccg agcgcggagc ccttgctggt cagcagccgc agaccggtgt gggtcggcag
     2401 gtcgcgggcc tcttcgagct cggtgccgcc gtgttcgaag agctcgccga cgtcttccgc
     2461 ggtgacctgg agctcggcca tgccggtcca cgtctcgtcg gattcggcga ccagctccgc
     2521 gcggtattcc tcggcggtca ggccgatcga ggcggccttg atccgcatcg gcaggtcttt
     2581 ggagaccagc acgacgtcga ggccctcgga ggccagattc tgcgcgacgg tcaggatgcg
     2641 cgtgtcgttg tcgcccagcc tgaacccggc cggcaggccg gatggatcgc tgtggttgag
     2701 ctcgaccctg atggttccct cgcccatcgg gatcggctcg tcgaggcgac cgtgctggac
     2761 acgcagatcg tccaggaaac gcaaggcttt gcgggcgaag tatcccagtt cgggatggtg
     2821 ccgcttacct tccagctctg tgatcacgac aacggggagc accacttcat gttcgtcgaa
     2881 ccggctgagt gcggccgggt ctgcgagcag gacagaggtg tccaggatgt acgtgcgccg
     2941 ggaggtgcca ggcgtgtgac cggacatgga cgggttggac aatgccacgc gttctccccc
     3001 gggcgcctgg ggtcggcgcc ctgccttgac tggtggtgaa cggaccgggc ccggacgcct
     3061 cgaaggcgga gccgggaacc ggcccctcct gcgaagtcat caagacgcaa gaacgggccc
     3121 tctcccgagg tgagcgtgga tgctcactcc ttacggtacg tcctgaatcc ccctgcgcca
     3181 acgctccaat ccttgccgaa agggggtgtt cacatgccgt ttcagaatcc gtaccgacgg
     3241 ttacgcgcgg cgtaggcgcg aagtgcccgc aagaagtcca ccctgcggaa atcaggccag
     3301 taggcctcgc agaagtagaa ctccgagtgg gcgctctgcc agagcaggaa tccggagagt
     3361 cgctgctctc ccgaggtccg gatgacgaga tccgggtcgg gcaggccacg cgtgtagaga
     3421 tgctcggcga tgtgctccac atcgaggacc tccacgaggt cttcgatgct cgcgcccttg
     3481 ctcgcgtgct cctggaggag ggagcgcacc gcatcagcga tctcacgtct tcctccatac
     3541 ccaacggcca cgttcacaat cagcccaggt cggtgagatg tcgcttcttt ggcatttttc
     3601 aggacatggg ccgtgtgatc gggcagcagg tcgagcgacc ccatcggctt gacgtgccag
     3661 ccctggtcga ccaggtcggt gaccaggttc tcgatcacct ggaggagcgg ttcgagctcc
     3721 ttcttgtcac gggagaggtt gtcggtggag agcagccaga gggtgacgta ctcgacgccg
     3781 gcctccttgc accagacgag gagctcggag atcttgtcgg cgcccttctg gtggccgtgg
     3841 cggacgtcct cgtggccgtt ggagcgggcc cagcggcggt tgccgtcgac gatcacaccg
     3901 acgtggcggg gggcctggat cgaggtgagc ttccgttcga gcctgcgttc gtacaccttg
     3961 taaacaagat cgcgcaggtc catgactccc ccggtgttcg cctgagcccg ctgtcacatg
     4021 acagacatgg tcaagcaatt atcgggggcg cgatgaggtc cccgctcccg aaatgaggga
     4081 gctgttacgt tccatcccat attgggttcc tagccggact tctgactcag tcaccaatgt
     4141 gctcaggaat atcaggagtt ctccggaagt gagcgtcgcc cgcaatccgg cccccgaagc
     4201 gccccaggcc tcgacgtggg cccgccgggt cagccgccac cggccggtga cgccccggcc
     4261 gggcacccag accgggatga gcctgagctg gcagcggagc tccccgccgc ccacgacgtc
     4321 ggtgcggctg cggtagcgga acccgaacag ttcctcctcg tcgtcgccgg ggaaccggtc
     4381 gtcggggtcg tcccaggccc cctgggaatc gcgcgcctcg gcgaccgacc gggccagccg
     4441 ggccagatac cagccgcacc gctccccgac cgagccgtag ggcacgccgt cgcggaacag
     4501 ctccaacgtg acctcgtagg gcaccccggc gctgtcgcgg catgccacgg gatcaaccgt
     4561 caggtacgaa ccgtcaacgc accgtagtcc acccacgcca cagagggtat cccgggcaca
     4621 ccccggcaaa ccggccctac cggtattgat ccttctttgg ggtaattcgc caccagtgcc
     4681 gcttttgttg gcgctccggc tcggcggcgg ccagcagccc ccgctccccc agccactcgg
     4741 cgaacgccgc cgcgtcggcc cgcgatccgc cctggggttt cggacggccg gaagcataag
     4801 cgtggaactc ccgcccgaac gcctgcccga gcgcccggac gacgtcgggc cgaagggctg
     4861 cgaccagccc ccgcctcttg gcgatcagac tgctcgcctg gatccgcagc cgctccgcgt
     4921 cgaacccgtg aggcggcgcg gcccccgcca ccagagccgc gagcagcgcc cgctgctgat
     4981 cggccagctg agcccgaggg tcagccacgg ttcagggccg ctctgatccg gtcgagttcc
     5041 tcggagagga ccgcgtcgct ggggtagtcg gcgtcccact ccaggagcgc gcccgggggc
     5101 cggtggcggg cggagagttc ggccaggagg tcgaggacct cctgcggcgg cgaggccgtg
     5161 tgggtgtcgt gccacacgtc gccgtgaccg tggcctcccg cgacgtggac gtaggcgacg
     5221 tgttccaggg gcagcgcgtc gagggccttg accgcgtcga ggcccaggtt gacctggttg
     5281 gtgtagaggt tggccacgtc gatcaggagc ttcaccccgg tccgttcgac cagttcgccg
     5341 aggaactgcg cctcggtgag ctcgtcgccg ggccagccga acagggccgc gacgttctcc
     5401 agggccagcg gcaccggcag gcagtcgagg gcgatgcgga cgttctccac gatcacgctc
     5461 agcgcgtcgc gggtgcgcgg caccggcagc aggtgcccgg cttcgaggtc tcccgaccga
     5521 acgtacgcga gatgttcgct caccagcgga gacccgaggg cctgggcgac ggcggccagg
     5581 tggtcgagcc gggcctggtc gggccgttcg gcgccgccga tgccgagcgc gacgccgtgg
     5641 gggatcaccg ggatcccccg cccgcgcatc agccgcagcg actcgggcac ccgcgccggg
     5701 acgatcgtct cggcgatgac ctcggtgaac tcgaccccgg gcaggtgctc gacggtcagg
     5761 tcgatctccg accgccagcc gatccccacg cccagccggt cagctgccac cgcagccacc
     5821 accgcaccct ccgccgcagc tcgacccgcc gccggagtcg ccgccgccgc ccgagtcgct
     5881 cccgccgccg ccgaagtcgc cgccgccgaa gtcggaaccg gagccccacc cggagtcggc
     5941 gcccaccgcg ccgccgcagg cgccggcgcc gcagccgcca ccgccgtgct ggatctcggc
     6001 gtggccgggg ccggtgtaga tggccaggcc gtagagggcg atcgtgctgt cgttgccgct
     6061 gttgtgggcg taggcgagct cgcgggtgcg cgggtagcgc gccttggccg cccgcagcgc
     6121 ggcgtgcccg gccggggtca gcacgctgag gttctccttg gcgttcccgg acgccaccgc
     6181 catcccgatg atccaggtga gcaccgagac gatcagcgtg agcacgctcc agagcaccga
     6241 ctcgaaccag ccgaacgcca gcgacacgat gctgaccggc acgaacagcc ccgcgagccc
     6301 gcacagccag cgcagcttgg tgttggcccg ccgggccgag gtgagcgccg agtcggccac
     6361 cacgagcccg tggccccgca gcccgtagtc gatgccggtg acggccgggc ccacgcagac
     6421 ggtccgccgg atgtcggcca gcggccggcc gttgaccccg gtcgcgaggt cgagcacctc
     6481 ctgctcgacc cgctcggcgg gggccccggc gatgccgcgg acggccgtca ccgagccgtc
     6541 gcgggagatc cgcacgcggc cctgggagac gagcacggcg accgcggtgt tgaccacccg
     6601 ccgggggccg cccgcgagga aggccagcga gtagggatcg aggccgggcg gagcgtagcc
     6661 gccgccggac ttcacccggc gctgctcctg cttgacccgg cgagcctgac ggaagaccag
     6721 caccacggcg acgatcgccg ggatgaacag gacgagggag tccatgccgc ctccatgacc
     6781 ccaggggaga gttacgactg attagacgat cccatggggc gccgggttcg cgcatcggcc
     6841 gtaataacgg ccatttcctc cgtcttcagc aggagaccac aaaaacgacg agacggctcg
     6901 cgccgtggat ccggctggac ccggcgcgag ccgtctcggg tgggggaatc ccgacgccgt
     6961 cggcgggact cctgctggtg gggtcacccc aatcgggcga ccagggagcg gtattcctcc
     7021 cagaggcctt cgggcaggtg gtcgccgaag gtctcgaagt gggccgggat cagggcggcc
     7081 tcctcgcgcc agacctcgcg gtcgacggtg agcagcaggt ccatgtcgtc ttcggcgatg
     7141 tccaggccct cggtgtcgat cttgacgggg agccgtccga tcggggtgtc gaccgcctcg
     7201 gcctggccgt tgaggcgttc gacgatccac ttgagcaccc ggatgttctc gccgaagccg
     7261 ggccagacga accggccctc gccgtccttg cggaaccagt tgacgtagta gatccgcggc
     7321 agcttcgcgc cctcggtctc gccgacgtcg agccagtggg cgaagtagtc gcccatgttg
     7381 tagccgcaga acggcagcat cgcgaacggg tcgcggcgca gctcgccgac cttgccctcg
     7441 gcggcggcgg tcttctccga ggcgacgttg gcgcccatga agacgccgtg ccgccagctg
     7501 cgggcctcgg tgaccagcgg caccgccgtg gcccggcggc cgccgaacag gatcgccgag
     7561 atcggcacgc cgttcgggtc ctgccactcg ggggcgatcg tcgggcactg gtgggccggc
     7621 gtggtgaacc gggcgttcgg gtgggcggcg agctcccccg agccgggcgt ccaggaatgg
     7681 ccgcgccagt cggtcaggtg ggccgggggc tcgtcggtca ggccctccca ccagacgtcg
     7741 ccgtcgtcgg tcagcgcgac gttggtgaag atgctgttgc cccacagcgt cgtgatcgcg
     7801 ttggcgttgg tggtctcgcc ggtgccgggc gcgacgccga agaagcccgc ctccgggttg
     7861 atcgcgtaga ggcggccgtc gtcgccgaag cgcatccagg cgatgtcgtc gccgatcgtc
     7921 tcgaccttcc agcccgggat gctcggcttg agcatggcca ggttggtctt gccgcacgcc
     7981 gacgggaagg ccgccgcgat gtagcgggtc tcgccgctgg gcggggtgag cttgaggatc
     8041 agcatgtgct cggccagcca gccctcgtcg cgggccatcg tgctggcgat ccgcagggcg
     8101 tagcacttct tgccgaggag ggcgttgccg ccgtagccgg agccgtacga ccagatctcc
     8161 cgggtctccg ggaagtggct gatgtacttg gtgtcgctgc acggccaggc gacgtcctcc
     8221 tggccggggg ccagcggggc gccgacggag tggacgcact tcacgtagtc gccgcgctcc
     8281 tcgatgagcc gcagggcctc ctcgcccatg cgggtcatca ccttcatcga gacggccacg
     8341 taggccgagt cggtgatctc cacgcccagc tgggagatcg ggccgcccag ggggcccatg
     8401 cagaacggga cgacgtacat cgtccggccg cgcatcgagc ccttgaacag ccgcccgaag
     8461 gtccgccgca tctcgccggg ctcgatccag ttgttggtgg gcccggcgtc cttctcccgc
     8521 tcggagcaga tgaacgtccg gtcctccacg cgggcgacgt ccgacgggtc ggaggcggcg
     8581 tggaagctgt tcgggcgggc cctgaggcgg gtcagcgtgc cctgcttcac gagcatgttg
     8641 gtgagccggg tccattcggc ctcggacccg tcgcaccact cgatccggtc gggctcggtg
     8701 agttccgcga tctcgctgac ccaggcggcc agttcggcgt gagtggtcgg atgcgaaagc
     8761 tgcagtgaca cggacacgag ggcggctcct taatgtacgt cagggccaat tcatcatccg
     8821 cgacgattac ccggtaaatc caattggtgt ggaccaccgg atattcgccg ccataaccgc
     8881 aggtcagcga agtagatgat ggatggagcg tacccctgac ggatgatttc caattgtgct
     8941 gaccacccct taccagttgt tctacgacta aacattggta taggccattg cgaatggtcc
     9001 agaccattcg gggaattggt tcagtccact ccgccggggc ccgcggcgcg cacgcggtcg
     9061 acggcctgga gggcatcgcg caggtcagcg agccaggtgt cggtgtggcg gccgaccagc
     9121 cggacgcacc aggcgagggc gtcggagcgg ctgcgcgcga cccccgcggc gacgagcgtg
     9181 tcgagcacct ggcgctcacc ctgccggagc cgggtcatga cggggacgga aagtgtggtg
     9241 aacatcacgg tctgctcacc gatcttcacg ccccaggcga ccttgcgccg aaacctgtgc
     9301 tccgcctggc gggcgatctc gatgcggcgc tcgcgggtct cctcacggaa ccgctcgacg
     9361 tagccctctt cggcggcggc ccgctcggcg tcgggcaggc cctcggtgac ggtcagcggg
     9421 ggaagcgcgc cgacgacggt gatctcctcg cggtcgacga cgacctcggg gcgctcggtg
     9481 aaccagttct cggggagacg gtgggcgaac cactcagatt ctgcactcat gtaatcaaca
     9541 ttacatcgtt gccagagaat cgggcggaga tattttgaca gtaactactt tttgagagtt
     9601 actatcaatt catggcaact tccagatggt gggctttgtc agccctggtc ctctccgtcc
     9661 tcgtgatcgg gctcgacgcg accgtgctca acgtcgcgct gcccaccctc gccgtcgatc
     9721 tcggcgccac caccggtgag ctccagtgga tcgtcgacgc ctatctcgtc gtgttcgccg
     9781 ccctgctgct gcccgccggg gtcttcggcg accgcttcgg gcgcaagagg ctcctgatcg
     9841 cgggcctggt ggcgttcggc gtcgcctcgc tgatcgcgat gctggccggc tcgaccagct
     9901 ggctgatcgc cgggcgggcg ctcatgggcg ccggggcggc gctgatcatg ccgctgtcca
     9961 cgtcgatcct gccgacgatc ttcccgcccg ccgagcgggc cagggccatc gccgtctgga
    10021 ccggcggcat ggccgtgggc ctgccgctcg gcccgatcgt cggcggctac ctgctcgacc
    10081 acttctggtg gggctcgatc ttcctgatca acctgcccgc cgtggcggtc gcgctggtcg
    10141 cggtggcctt cctggtgccg gagtcccgcg ccgccgagaa gcgcggcacc gacgtgcccg
    10201 gcacgctcct gtcggtcgcc gggctcgccg cactcgtctt cggcgtcatc gaggccccgg
    10261 tgcggggctg gggctcggcc ctggtgctcg gctcgatcgc cgccgggctc gtgctgctct
    10321 tcctgttcgt acgggtggag gcccgctccg ccgccccgat gatggacctg gggctgttcc
    10381 gcgaccgcac cttcgtgtgg ggcgctctcg gcgcgaccgt ggtctcgctc ggcatgatgg
    10441 gcgtcctgtt cgtcgcgccg ctctatctcc aggcggtgca gggcttcgac gccatggcca
    10501 ccggcctgcg catcgtgccc atgatcggcg ggctcatggc aggcggtctc gtcggcgaac
    10561 gcctgatcaa gcgcctcagc ctgcgggtca tcatgggtgc cgggctggtc gtgctggccg
    10621 ccgggctgat ggcgggggcg gtgctgccgg gcgactacac gtacacggcg atctggctca
    10681 ccgtcaccgg cgcgggcgtg ggcctgacga tggtcccggc catggacggc ctgctcgccc
    10741 tcctcccgcc ggagcgcacc ggcatgggca gcggcgtcct gcaggccctg cgccagaccg
    10801 gcggcgcgtt
//
