LOCUS       NZ_BBXG01000009        17772 bp    DNA     linear   CON 08-AUG-2024
DEFINITION  Herbidospora cretacea strain NBRC 15474, whole genome shotgun
            sequence.
ACCESSION   NZ_BBXG01000009
VERSION     NZ_BBXG01000009.1
KEYWORDS    WGS; HIGH_QUALITY_DRAFT; RefSeq.
SOURCE      Herbidospora cretacea
  ORGANISM  Herbidospora cretacea
            Bacteria; Actinomycetota; Actinomycetes; Streptosporangiales;
            Streptosporangiaceae; Herbidospora.
REFERENCE   1
  AUTHORS   Komaki,H., Ichikawa,N., Oguchi,A., Hamada,M., Tamura,T. and
            Fujita,N.
  TITLE     Genome-based analysis of non-ribosomal peptide synthetase and type-I
            polyketide synthase gene clusters in all type strains of the genus
            Herbidospora
  JOURNAL   BMC Res Notes 8, 548 (2015)
   PUBMED   26452464
  REMARK    DOI:10.1186/s13104-015-1526-9 Publication Status: Online-Only
REFERENCE   2  (bases 1 to 396994)
  AUTHORS   Hosoyama,A., Oguchi,A., Ichikawa,N., Kimura,A., Ohshita,R. and
            Fujita,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAY-2015) Contact:Director-General Department Of
            Biotechnology National Institute of Technology and Evaluation,
            Biological Resource Center (NBRC); 2-49-10 Nishihara, Shibuya-ku,
            Tokyo 151-0066, Japan URL :http://www.nite.go.jp/nbrc/index.html
COMMENT     ##Genome-Assembly-Data-START##
            Finishing Goal                    :: Noncontiguous Finished
            Current Finishing Status          :: High-Quality Draft
            Assembly Method                   :: newbler v. 2.6
            Genome Coverage                   :: 71x
            Sequencing Technology             :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_001570605.1-RS_2024_08_08
            Annotation Date                   :: 08/08/2024 02:39:29
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,566
            CDSs (total)                      :: 7,487
            Genes (coding)                    :: 7,430
            CDSs (with protein)               :: 7,430
            Genes (RNA)                       :: 79
            rRNAs                             :: 4, 5 (5S, 16S)
            partial rRNAs                     :: 4, 5 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 10 of 57
            Pseudo Genes (incomplete)         :: 49 of 57
            Pseudo Genes (internal stop)      :: 3 of 57
            Pseudo Genes (multiple problems)  :: 4 of 57
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 12:10:14
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 379222
            Orig. end                         :: 396994
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            BBXG01000009.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..17772
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="terpene-precursor"
                     /region_number="2"
                     /rules="(PT_FPPS_like and not PT_HPP)"
                     /tool="antismash"
     cand_cluster    1..17772
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(PT_FPPS_like and not PT_HPP)"
                     /kind="single"
                     /product="terpene-precursor"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..17772
                     /aStool="rule-based-clusters"
                     /category="terpene"
                     /contig_edge="True"
                     /core_location="[10000:10933](+)"
                     /cutoff="10000"
                     /detection_rule="(PT_FPPS_like and not PT_HPP)"
                     /neighbourhood="10000"
                     /product="terpene-precursor"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      10001..10933
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="10000"
                     /detection_rule="(PT_FPPS_like and not PT_HPP)"
                     /neighbourhood="10000"
                     /product="terpene-precursor"
                     /protocluster_number="1"
     misc_feature    complement(1345..1358)
                     /note="TFBS match to ANR, Anaerobic transcriptional
                     regulator, confidence: weak, score: 19.57"
                     /tool="antismash"
     gene            1646..1942
                     /locus_tag="AW270_RS20845"
     CDS             1646..1942
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /GO_function="GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /GO_function="GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020542548.1"
                     /locus_tag="AW270_RS20845"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="WhiB family transcriptional regulator"
                     /protein_id="WP_061296783.1"
                     /transl_table=11
                     /translation="MWITDWTSRAACRGADPDALFVQGAAQNRAKLICRGCPVRTECLA
                     DALDNRIEFGVWGGMTERERRALLRRRPDVDSWRDLLELAKEEYERTNELIAG"
     gene            complement(1946..3076)
                     /locus_tag="AW270_RS20850"
     CDS             complement(1946..3076)
                     /codon_start=1
                     /gene_functions="other (smcogs) SMCOG1157: hypothetical
                     protein"
                     /gene_kind="other"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012855220.1"
                     /locus_tag="AW270_RS20850"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="ArsA family ATPase"
                     /protein_id="WP_061296784.1"
                     /transl_table=11
                     /translation="MTTPPTPPELDLDGIIDDPGTRIVVCCGSGGVGKTTTAAALGLRA
                     AERGRNVVVLTVDPARRLAQSMGLTSLDNTPRKVAGADGELFAMMLDMKRTFDEIIEAH
                     ADPERARQILTNPFYQSLSSSFSGTQEYMAMEKLGQLRRSGEWDLIIVDTPPSRSALDF
                     LDAPERLGRFLDGKLIRLLMAPAKAGGRSAFKILNAGFGLVAGVLTKVLGAQVLKDIQT
                     FVSALDAVFGGFRQRAEQTYKLLQAPGTAFVVVATPERDAMREASYFVERLAEERMPLA
                     GLVVNRVHRSPAPGLTAARSTAAAEDLESRGEHELTAAVLRLHADRMQLAAREHREQER
                     FASAHPTVPATQVPAMSQDVHDLDGLREIGELLAKR"
     misc_feature    3142..3157
                     /note="TFBS match to LexA_variant_1, Repressor of DNA
                     damage response, confidence: medium, score: 24.49"
                     /tool="antismash"
     misc_feature    complement(3143..3156)
                     /note="TFBS match to LexA_variant_2, Repressor of DNA
                     damage response, confidence: weak, score: 18.28"
                     /tool="antismash"
     gene            3190..>4367
                     /locus_tag="AW270_RS20855"
                     /pseudo=""
     CDS             3190..>4367
                     /codon_start=1
                     /gene_functions="other (smcogs) SMCOG1192: transposase"
                     /gene_kind="other"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019607257.1"
                     /locus_tag="AW270_RS20855"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /product="RNA-guided endonuclease InsQ/TnpB family protein"
                     /pseudo=""
                     /transl_table=11
                     /translation="MAQIVKRAYKYRFYPTPEQAEELARTFGCVRLVYNKALEERTRAY
                     RLEGRKLSYAESSAALTAWKRTEELSFLNEVSCVPLQQALRHQQMAFSNFFARRAGHPV
                     FKSRKKSRSSAEYTRSAFRWREGNLTLAKMDGPLDIVWSRPLPEGAKPSTVTVSRDAAG
                     RWFVSILVEEKIAPLSPAGSMIGVDVGISALATLSTGERLANPRYEASDRRKLARAQRA
                     LARKSPGSGNRARAKLAVARIYARIADRRRDYLHKVTTRLVRENQVIAVEDLTVANMVK
                     NRSLARAISDASWRELRSMLAYKASWYGRELLVVDRWFPSSKLCSACGVLTALMPLDVR
                     EWSCGCGAAHDRDVNAAKNILAAGLAER"
     gene            complement(4417..5397)
                     /locus_tag="AW270_RS20860"
     CDS             complement(4417..5397)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019633549.1"
                     /locus_tag="AW270_RS20860"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="ArsA-related P-loop ATPase"
                     /protein_id="WP_061296786.1"
                     /transl_table=11
                     /translation="MRSRDTDWDGVRLHVVTGKGGTGKTTVAAALALALAAGGRKVLLV
                     EVEGRQGIAQVFDLPPLPYEERKIAVAPGGGDVHALAIDPEEAMLEYLEMFYGLKRMGK
                     ALTKMGVVDFATTIAPGLRDVLVTGKTSEAVRRRDRTGKRVYDTVVMDAPPTGRVTRFL
                     NVTQEVAGLARVGPVRAHADMVRGVVASPETAVHFVTLLEEMPVQETLDGVTELRAAKL
                     PVGGIFVNMVRTVDPVLEGPFDVAELALGVKAAGLGDDGNAAVIAEALGGEVAEQQRRA
                     ELEITERDALETAGVPRYELPLLADGADLAGLYELAEAIRTQGAA"
     misc_feature    complement(5403..5421)
                     /note="TFBS match to DmdR1, Iron(II)-dependent repressor,
                     confidence: weak, score: 21.38"
                     /tool="antismash"
     gene            5467..5622
                     /locus_tag="AW270_RS39655"
     CDS             5467..5622
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017621623.1"
                     /locus_tag="AW270_RS39655"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_169807346.1"
                     /transl_table=11
                     /translation="MTKWEYVTVPLLTHATKQILDNWGDDGWELVAVVPGPNPEQLVAY
                     MKRAKA"
     gene            5619..6074
                     /locus_tag="AW270_RS20865"
     CDS             5619..6074
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014913527.1"
                     /locus_tag="AW270_RS20865"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="RidA family protein"
                     /protein_id="WP_061296787.1"
                     /transl_table=11
                     /translation="MTSPEDRLSALGLSLPEVAAPLAAYVPAVRSGSHVYTSGQLPIAA
                     GELLKTGKVGAEVTPEEAYELARVCALNAIAALKSVVGDLGQVAGIVKVVGFVASTPDF
                     TGQPQVVNGASELLGEVFQGAHARSAVGVAVLPRDAPVEVELIAEIR"
     gene            6096..6872
                     /locus_tag="AW270_RS20870"
     CDS             6096..6872
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020542553.1"
                     /locus_tag="AW270_RS20870"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="NUDIX hydrolase"
                     /protein_id="WP_061296822.1"
                     /transl_table=11
                     /translation="MALPGELGERARDLLAGRLVPVPARAAATVALLREPPLEVYLLRR
                     VATMAFAAGAYVFPGGSVDPRDAGPDIGWAGPSPAEWGDLLNADEDTARGLVCAAVRET
                     FEESGVLLAGDPVDDTTMWEAERLALISRELSFAEFLAANGLVLRSDLLKPWAHWITPE
                     IETRRFDTRFFVAALPPGQRTRDVGGEADTVVWTSPADALTSGYFLMPPTLHTLSALAA
                     AGTITDVFAADPKITTVMPAPVERNGQMVLTTEGQI"
     gene            6872..7678
                     /locus_tag="AW270_RS20875"
     CDS             6872..7678
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1170: metallo-beta-lactamase family protein"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020542554.1"
                     /locus_tag="AW270_RS20875"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="WP_061296788.1"
                     /transl_table=11
                     /translation="MSGLSLPIEGPIEGPIDGSGTATAVNVLAPNASPMTLDGTNTWVL
                     GRGESVAVVDPGPDDPGHLSTVVSRLGGRNVEVILLTHGHEDHSGGARRFAEMVKSPVR
                     ALDRTHVLGEEGLEDGDALTAGGVEIRVIGTPGHSFDSLCFWLPEDGALLTGDTILGRG
                     TTVIAPDGDLADYLRSLDRLRAMAELSEAESLLPGHGPILADPIGKLDEYITHRRQRLD
                     QIRQAIRDGAKDAREIVEMVYAEVPRELWPAAEMSVAAQLNYLKKL"
     gene            complement(7679..8359)
                     /locus_tag="AW270_RS20880"
     CDS             complement(7679..8359)
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_function="GO:0030552 - cAMP binding [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1133:
                     Transcription regulator, crp"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017578423.1"
                     /locus_tag="AW270_RS20880"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="WP_061296789.1"
                     /transl_table=11
                     /translation="MNTEEVLSRAPLFSALDRESAAALRTSITEVTLTKGQTLFSENET
                     GATLYVVLEGKIKLARTASDGRENLLSVLGPSEMFGELSLFDPRPRTASAIALTDVKLA
                     GLSHDDLRPWLTGRPEVALHLLRALAQRLRRTNDVLADLVFTDVPGRVAKALLDLAERF
                     GQKVEDGIRVHHDLTQEELAQLVGASRETVNKALADFAGRGWLRIEAKAVVIMDMERLY
                     NRSR"
     gene            complement(8401..8991)
                     /locus_tag="AW270_RS20885"
     CDS             complement(8401..8991)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1057: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: protein motif:HMM:NF037675.4"
                     /locus_tag="AW270_RS20885"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="WP_063819276.1"
                     /transl_table=11
                     /translation="MPGKADATRQRLLDAAMEEFAAYGIAGARVDRIAAVAGTNKAQIY
                     HYFGSKDQLFDAVFTGIVETVVRETPIDVHDLPGYAAALARGYDAHPQIARLATWQRLE
                     RADDPPNPRAVESTRAKVEAIAKAQAEGVLPAVFPAGVLLGLLLSLASAWTSVTPHYTA
                     AVETLTPDERAGYVEAAVRRLLSGSPGDTEGGA"
     misc_feature    complement(9055..9070)
                     /note="TFBS match to RutR, Regulator of the pyrimidine and
                     purine metabolism, confidence: weak, score: 20.73"
                     /tool="antismash"
     gene            9083..9976
                     /locus_tag="AW270_RS20890"
     CDS             9083..9976
                     /NRPS_PKS="Domain: PKS_KR (3-171). E-value: 6.7e-15. Score:
                     47.4. Matches aSDomain:
                     nrpspksdomains_AW270_RS20890_PKS_KR.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) adh_short"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1001: short-chain dehydrogenase/reductase SDR"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /locus_tag="AW270_RS20890"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="WP_063819277.1"
                     /sec_met_domain="adh_short (E-value: 3.3e-34, bitscore:
                     113.0, seeds: 230, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIWFVTGSSRGLGREIVLAALERGDSVAATARTPAQLDDLSQKYG
                     DRVLALPLDVTDAEAVEDAVATAVEAFGTLDVVVNNAGWADMSAFEDTPIDNFRAQVET
                     NFFGVVNVTRSVAPILRRQGHGSLINVSSIGGRLGTPGLSAYQASKWAVNGFTEVIAQE
                     LGPLGVRVTTIEPGGMTTDWGGASMTIPPISEPYEQSVGAMVRLLRQGERKPLGDPAKV
                     AQVIVRITEMDEPPVRLLLGSDALNGARYAAHTTAERDAAYTDLSRSTDRDDATAQERD
                     PLGQAFSTSADGAAVE"
     aSDomain        9092..9595
                     /aSDomain="PKS_KR"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_AW270_RS20890_PKS_KR.1"
                     /evalue="6.70E-15"
                     /label="AW270_RS20890_PKS_KR.1"
                     /locus_tag="AW270_RS20890"
                     /protein_end="171"
                     /protein_start="3"
                     /score="47.4"
                     /specificity="KR activity: inactive"
                     /specificity="KR stereochemistry: (unknown)"
                     /tool="antismash"
                     /translation="FVTGSSRGLGREIVLAALERGDSVAATARTPAQLDDLSQKYGDRV
                     LALPLDVTDAEAVEDAVATAVEAFGTLDVVVNNAGWADMSAFEDTPIDNFRAQVETNFF
                     GVVNVTRSVAPILRRQGHGSLINVSSIGGRLGTPGLSAYQASKWAVNGFTEVIAQELGP
                     LGVRV"
     CDS_motif       9476..9526
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_AW270_RS20890_0001"
                     /evalue="6.60E-04"
                     /label="PKSI-KR_m4"
                     /locus_tag="AW270_RS20890"
                     /protein_end="148"
                     /protein_start="131"
                     /score="12.2"
                     /tool="antismash"
                     /translation="SSIGGRLGTPGLSAYQA"
     gene            10001..10933
                     /locus_tag="AW270_RS20895"
     CDS             10001..10933
                     /EC_number="2.5.1.-"
                     /GO_function="GO:0004659 - prenyltransferase activity
                     [Evidence IEA]"
                     /GO_function="GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /GO_process="GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     terpene-precursor: PT_FPPS_like"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1182: Polyprenyl synthetase"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF012568.4"
                     /locus_tag="AW270_RS20895"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="polyprenyl synthetase family protein"
                     /protein_id="WP_061296791.1"
                     /sec_met_domain="PT_FPPS_like (E-value: 1.7e-40, bitscore:
                     133.4, seeds: 145, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTDSMTIRDVLNSTRELIEPAHRAAIDRLSPELRHVAGYHIGWWD
                     DEGNELIGGQGRFVRPALALGCARAAGGHAADAVPMAVATELVNDFSLLHDDVIDGDDI
                     RRERPAAWTVFGLSEALLTGDALLAVAMALVGPAGVRTLADALLDLCHGQASDLAFVDR
                     PQVTLAECMTMAENKTGALLGAACELGALAGGAGDLQVFREFGTRLGLAFRLTDDVRKL
                     VADLGCRRKSLPVVAALESGTPPAARLAESLRDGGDPETQADLVERTGAREWALREADR
                     QVELAFTALGHGATPELRLLADLSLGRTR"
     gene            complement(10930..11400)
                     /locus_tag="AW270_RS20900"
     CDS             complement(10930..11400)
                     /GO_function="GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020546078.1"
                     /locus_tag="AW270_RS20900"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="CoA-binding protein"
                     /protein_id="WP_061296792.1"
                     /transl_table=11
                     /translation="MRYQEPQDIQRIVNTAKTVAVVGLSGNELRASNFVGFYLKRHGYR
                     MIPVNPREKEILGETSYPSLSDIPVKVDVVNVFRAPDAVPGIAREAVAIGAGALWLQFG
                     VISEEGARIAEEGGLTVIMDRCMKIEHARYMGRMHWLGFNTGRITSVRTGIH"
     gene            complement(11397..12710)
                     /locus_tag="AW270_RS20905"
     CDS             complement(11397..12710)
                     /GO_function="GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /GO_function="GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /GO_process="GO:0019346 - transsulfuration [Evidence IEA]"
                     /NRPS_PKS="Domain: Aminotran_5 (77-226). E-value: 3.4e-11.
                     Score: 34.6. Matches aSDomain:
                     nrpspksdomains_AW270_RS20905_Aminotran_5.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_1_2"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) DegT_DnrJ_EryC1"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_5"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1168: O-succinylhomoserine sulfhydrylase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020546079.1"
                     /locus_tag="AW270_RS20905"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase family
                     protein"
                     /protein_id="WP_061296793.1"
                     /sec_met_domain="Aminotran_1_2 (E-value: 4e-09, bitscore:
                     30.6, seeds: 48, tool: rule-based-clusters)"
                     /sec_met_domain="DegT_DnrJ_EryC1 (E-value: 1.3e-13,
                     bitscore: 45.4, seeds: 38, tool: rule-based-clusters)"
                     /sec_met_domain="Aminotran_5 (E-value: 9.6e-10, bitscore:
                     32.4, seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSDRPFRTEEAREFGFETRQLHAGQRPDPNTGARAVPIFQTTSYV
                     FEDPESAAAYFNLQEYGNTYSRIMNPTTAVFEERMANLEGGTGAVAFASGIAAQAASLF
                     TLLEPGDHVVSSSALYGGTVNQLKHLFRKMNVELTWVDPDDPEAWRAAVQPNTKAFYGE
                     TIGNPAGNVLDIETVAAIAHDNGLPLIVDNTFATPYLCRPIEWGADIVVHSATKFIGGH
                     GTSIGGVVVEAGTFDYSNGRFPVIAQPSPAYHGLKFHETFGEYGYLMKLRAETLRDLGA
                     AMSPFNAFLFLQGLETLSLRMDRHVDNALAVAEFLQAHELASKVTYPGLADSSYKALVE
                     KYLPRGAGAVFSFECAGGREGGQAFIRGTTLWSHLANVGDAKSLIIHPASTTHRQLGDA
                     ELAAAGVGAGTIRLSVGVESVADLIWDLEQGFAHVEKA"
     aSDomain        complement(12033..12479)
                     /aSDomain="Aminotran_5"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_AW270_RS20905_Aminotran_5.1"
                     /evalue="3.40E-11"
                     /label="AW270_RS20905_Aminotran_5.1"
                     /locus_tag="AW270_RS20905"
                     /protein_end="226"
                     /protein_start="77"
                     /score="34.6"
                     /tool="antismash"
                     /translation="ERMANLEGGTGAVAFASGIAAQAASLFTLLEPGDHVVSSSALYGG
                     TVNQLKHLFRKMNVELTWVDPDDPEAWRAAVQPNTKAFYGETIGNPAGNVLDIETVAAI
                     AHDNGLPLIVDNTFATPYLCRPIEWGADIVVHSATKFIGGHGTSI"
     gene            complement(12707..13891)
                     /locus_tag="AW270_RS20910"
     CDS             complement(12707..13891)
                     /EC_number="2.3.1.31"
                     /GO_function="GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /GO_process="GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /NRPS_PKS="Domain: Abhydrolase_1 (45-375). E-value: 5e-27.
                     Score: 87.0. Matches aSDomain:
                     nrpspksdomains_AW270_RS20910_Abhydrolase_1.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF00561"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020546080.1"
                     /locus_tag="AW270_RS20910"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="homoserine O-acetyltransferase"
                     /protein_id="WP_245674788.1"
                     /sec_met_domain="PF00561 (E-value: 2.6e-38, bitscore:
                     126.6, seeds: 48, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MGSIGFVETQYLDLPGPIRLDSGRELRDVRVAYETYGTLSPERDN
                     VILVCHALSGNAHAAGSAKTAPEASARDGFGAEDRDGVKGKGLGWWDGMIGPGKAFDTD
                     RYFVVCTNLLGGCSGTTGPSTIDSATGRPYGSDFPVVTVADMVRTERAFLDELGIERLA
                     VIAGGSLGGMQALEWAVQYPGQVDSIVVIASTHALHPQGLAWNAIARESIMRDPDWQGG
                     HYYGTGRKPDAGMGVARMVGHVTYLSGVALTDKFGRRLQFSDDIRHTIVEPEFEVESYL
                     RHQADSFVKRFDANSYLYISRALTYFDLAREHGGGSLEKALENVQARTLLISFSSDWLY
                     PPEASDEIAAALTNLGKPVENHLIDAPYGHDCFLLEEARQTPLIRQFLTRKMAS"
     aSDomain        complement(12767..13756)
                     /aSDomain="Abhydrolase_1"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_AW270_RS20910_Abhydrolase_1.1"
                     /evalue="5.00E-27"
                     /label="AW270_RS20910_Abhydrolase_1.1"
                     /locus_tag="AW270_RS20910"
                     /protein_end="375"
                     /protein_start="45"
                     /score="87.0"
                     /tool="antismash"
                     /translation="VILVCHALSGNAHAAGSAKTAPEASARDGFGAEDRDGVKGKGLGW
                     WDGMIGPGKAFDTDRYFVVCTNLLGGCSGTTGPSTIDSATGRPYGSDFPVVTVADMVRT
                     ERAFLDELGIERLAVIAGGSLGGMQALEWAVQYPGQVDSIVVIASTHALHPQGLAWNAI
                     ARESIMRDPDWQGGHYYGTGRKPDAGMGVARMVGHVTYLSGVALTDKFGRRLQFSDDIR
                     HTIVEPEFEVESYLRHQADSFVKRFDANSYLYISRALTYFDLAREHGGGSLEKALENVQ
                     ARTLLISFSSDWLYPPEASDEIAAALTNLGKPVENHLIDAPYGHDCFLL"
     misc_feature    complement(13904..13922)
                     /note="TFBS match to LexA_variant_3, Repressor of DNA
                     damage response, confidence: weak, score: 20.07"
                     /tool="antismash"
     gene            13941..14480
                     /locus_tag="AW270_RS41335"
     CDS             13941..14480
                     /EC_number="1.11.1.-"
                     /GO_function="GO:0004601 - peroxidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020546081.1"
                     /locus_tag="AW270_RS41335"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="OsmC family protein"
                     /protein_id="WP_061296795.1"
                     /transl_table=11
                     /translation="MTNGVNVQALLDAREVLKGAPEAAQFTWRASSKWQHGVHSTTRIE
                     KFFGLGQEQSHKSESVFEADHPAIFAAEDNGITPIEYLLVGLASCLTAGIASVAQNRGI
                     KLNSVEASVEGGHDIRGILGADSDVRNGFNDIKVTFSIDADAPRADIEALVAQSQKRSA
                     VFDALTNPTNVTVEVA"
     gene            <14384..15673
                     /locus_tag="AW270_RS20920"
                     /pseudo=""
     CDS             <14384..15673
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Pyr_redox_2"
                     /gene_functions="other (smcogs) SMCOG1092: hypothetical
                     protein"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF025114.4"
                     /locus_tag="AW270_RS20920"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /product="NAD(P)-binding domain-containing protein"
                     /pseudo=""
                     /sec_met_domain="Pyr_redox_2 (E-value: 1e-13, bitscore:
                     45.7, seeds: 64, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="RRHRGSRRPVAEAFGRLRRADQPDERHRRSRLIVNVTTAVIGAGH
                     AGLAASHFLTAASIDHVVLERGEVANSWRHERWDSLRLLTPNWLNRLPGSHYEGPDPDG
                     YADMGEVVDFLERSAKDVPVRTGVTVTSVRRSAGGYTLTTDQGPVEARTVVIASGACNV
                     ASVPALAAAVPSSVEQVTPFGYRNPSRLPDGGVLVVGASATGVQLAAEIRRSGRPVVLS
                     VGEHVRMPRVYEGRDVLWWMHNSGVWSQRYDEVDDLTRARRLPSPQLAGASTLDLNVLT
                     ALGVELVGRLSAIRDGAALFSGGLRNVAALADLKMNRLLDTFDQWAGTAPGERFAPTDV
                     PGTARWRVDLGSGEIRSIVWATGFRPDYSWLDVPVVDPKGALTHDGGVLGDGLYALGLP
                     VLRRRRSTFICGIEDDAREVVDHLRLHLAG"
     gene            complement(15670..16569)
                     /locus_tag="AW270_RS20925"
     CDS             complement(15670..16569)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="AW270_RS20925"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_061296796.1"
                     /transl_table=11
                     /translation="MNWLRDNDPACHVRADPATMAAVLDTIVRTPRPVRRRRRRSLVLI
                     PLTALTAILVIMSQPGVSASYELGPAKSVAFSRNSDRIDIRIADPNADPERFRADFAAH
                     GLDVTLDTVPTSPSLVGAVISVNSPQRMVHYHTDGFEIVGEQGSEWITSIRGTRCGDRS
                     CVTAVSVPVDLRSPLELTIGRAPEPGERYEVGGDPTAPGEPLEGFDLTNYTVDEAARLL
                     RELNVTVQSYHPERPPDHVGGWDYSAHTVQPEKVPGSWHVQRVFGGHAPDTVRLVVAAE
                     PTDRPFWEFVDSLMFWRD"
     gene            complement(16566..17057)
                     /locus_tag="AW270_RS20930"
     CDS             complement(16566..17057)
                     /GO_function="GO:0016987 - sigma factor activity [Evidence
                     IEA]"
                     /GO_process="GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1032: RNA
                     polymerase, sigma-24 subunit, ECF subfamily"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /locus_tag="AW270_RS20930"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="RNA polymerase sigma factor"
                     /protein_id="WP_061296797.1"
                     /transl_table=11
                     /translation="MSDEADFTRMFKEHYTPVLRYAWRRAGPADAADIAAETFKIAWEK
                     YDRVPRDQPLPWLYATARNLTLNLIRRTHRRDALTEWAGGAEEDHAAAVTDRQAALAAL
                     NSLSEGDRELVLLMSWEGLDLGQAAKVVGCSRATAAMRVHRLRRRLRHLFVEEPERSVR
                     "
     misc_feature    complement(16620..16622)
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     CDS             complement(17252..>17768)
                     /codon_start=3
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="AW270_RS39660"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_169807347.1"
                     /transl_table=11
                     /translation="PTPTPTSTPKAPCWAGSWTVTTATAEVAGKKHNFFAQGGAGIKAT
                     FTPEGKYVGAHYDFRTAALLTGGATFRLKGRENFTYTFGGHGPQTYGKVALKPRTGNIG
                     ARLERSGGGKGFSGLLRDAVARNEDLGAIPRKGTYTCVPGKSLRIDTHGKSGRTVITIK
                     WELTRTPVV"
     gene            complement(17252..>17772)
                     /locus_tag="AW270_RS39660"
ORIGIN
        1 ctggagttga acacctggtg ggtctcgccg gcgcggttgc ccttgcagtc gcggaagggc
       61 gacagcgagt tggtcgggat gtcgtaggtc ttgccggtgg cgaacccgtc gttgagcttc
      121 atgccgttgt cgagggcggt cagcagggtg atcaccttga acgtggaacc cgcctggaag
      181 ccggaaccgc cgccgtgggc gcggttggcc gccaggttgt agctcatctc gttcttcttc
      241 ttgttgtcac cgaacttgcg gctggcggcc agcgccttga tcgcgcccgt gcccggttcg
      301 accagcgcct cggccgcgac ctgctcgtcg ctcgcgtaga cgtgcttctt gatcgcgtcg
      361 ttggcggcct tctgcatctt cgtgtcgagc gtggtgcgga tgaccaggcc accgcggttc
      421 aggaagtcgc gccgggcctt ctcggtcttg ccgaagtcgg ggttgttcag gatgtcgttc
      481 tgcacgtaga ggcagaagaa cgggtaggag ctgtcctcgc agccaccggg catcttctcg
      541 cccttgtagc cgagcttctc cttcttggcc tcggccgcct gcgccacggt gatcttcccg
      601 agttcggcca tccggtcgag cacggtgttg cggcgggcga gcagccgctc gcggaactcc
      661 ttgccccggc ccgggtcggt ctggctcggg ttctgcaccg cgccggccag cgtcgcggcc
      721 tcgtgcagct ccagctcaga ggcgtgcttg tcgaagaagc gcttggacgc cgcctcgatg
      781 ccgtaggcgc cgtcgccgaa gtaggagatg ttcaggtagc ggttgaggat ctcgtccttc
      841 gagtacttct tctcgatcgc catggcgtag cggatctcgt tgagcttgcg cgtgtacgtc
      901 gcctcgatgg ccttgagctg ctcttcctcg gtctcggccg agttgaagat gaccagcttg
      961 acgtactgct gcgtgatcga cgagccaccc tgcacgaccc cgccgccgaa gacgttcttc
     1021 gtgagcgcgc ggaccgttcc ctcgatgtcg agggcgccgt gctcgtagaa gcggaagtcc
     1081 tcgatcgcga tgatggcttg ctgcatcacc ggcgcgatct tgctcagcgg caccgactcg
     1141 cggttctgga agtagaactg cgcgatctgc ttgccgtcgt tgtcgagcag gacggtcttc
     1201 tcgggcagcg ggggctcgtc gagctcctcc gcctcgatgc gcaacgactc gacgcccgac
     1261 ttcgcgccga acccggctcc acccacggcg ggcagcccga cggccgcaac gatcacgccg
     1321 gccatggcgg cggcggcgac cagcttgacc agcgtcaaga tcgcgttccc gaaacgggct
     1381 ttgcctgaag cttgcacgga gctaagcgta cgtcgaagta aggggagatt cggtaccaaa
     1441 cgcaccacgt cgcattaccc gcgcttggga tgactagtca tacccggaca ccgccgcccg
     1501 ccaaatttgg tccctgcggg tctgtccatt cggatgcgga gtctcgtacg ttctcctctg
     1561 cggcaactga ataaggaggc tcggcgaccg cgcccgtcgg ccccagaagt cacgactgac
     1621 cacctaaggg gagtggggtc gaaaaatgtg gatcacggat tggacctccc gtgccgcctg
     1681 tcgaggtgcg gatcctgacg ctctgttcgt tcagggcgcc gcacaaaacc gggcgaagtt
     1741 gatctgtcga ggatgccctg tccgtaccga atgcctcgcc gatgcgctgg acaaccgcat
     1801 cgagttcggg gtttggggcg gaatgaccga acgggaaagg cgcgccctcc tgcgccgtcg
     1861 gcccgacgtc gactcctggc gtgacctgct ggagctggcg aaggaagaat acgaacgcac
     1921 taatgagctg atcgccggct gaccctcacc gcttggccag gagttcgccg atctcacgca
     1981 acccgtcgag gtcgtggacg tcctgtgaca tcgcgggcac ctgggtggcc ggcacagtgg
     2041 ggtgggccga agcgaaacgt tcctgctccc ggtgctcgcg agcggcgagt tgcatccggt
     2101 cggcgtgcag tctcagtaca gcggcggtga gctcgtgttc accacgtgac tccaggtctt
     2161 ccgcggcggc ggtgctccgc gccgcggtaa gaccgggagc cggtgagcgg tgcacccggt
     2221 tgaccaccag accggccagc ggcatccgct cctcggccag ccgctccacg aaataggagg
     2281 cctcccgcat cgcgtcacgc tccggcgtgg ccaccacgac gaacgcggtg ccaggagcct
     2341 gcaacagctt gtaggtctgc tccgcccgct ggcggaatcc accgaagacg gcgtcgagcg
     2401 cggagacgaa cgtctggatg tccttcagca cctgggcgcc gagcaccttc gtgagcaccc
     2461 cggcgacgag gccgaacccg gcgttcagga tcttgaacgc gctgcgcccg cccgccttgg
     2521 cgggggccat cagcagccgg atcagcttcc cgtcgaggaa ccgcccgagc cgttcgggcg
     2581 cgtcgaggaa gtcgagcgcc gaccgtgacg gaggcgtgtc gacgatgatc agatcccact
     2641 cgcccgaccg ccggagctgg ccgagcttct ccatggccat gtattcctgc gtgccggaga
     2701 agctcgacga gagcgactgg tagaaggggt tcgtcaggat ctggcgggcc cgctcggggt
     2761 cggcgtgggc ctcgatgatc tcgtcgaagg tccgcttcat gtcgagcatc atggcgaaca
     2821 gctcgccgtc ggcccccgcc accttgcgcg gggtgttgtc gagcgaggtc agccccatcg
     2881 actgggccag ccggcgggcg gggtcgacgg tcagcaccac gacgttgcgg ccccgctcgg
     2941 cggcccggag cccgagggcg gccgccgtgg tcgtcttgcc gacaccgccc gatccgcagc
     3001 agaccacgat ccgggtcccc ggatcgtcga tgatgccgtc gaggtcgagc tcaggcggcg
     3061 tcggaggcgt ggtcatcaga actcctggag cagggtcggt gccgtgggca ggcgggagag
     3121 gtgtttcttg gtgtcacagg atcgaacttg tgtgcgatta tcgactgatg gcgggttgac
     3181 ttctctaccg tggcgcagat cgtgaagcgg gcctacaagt accgcttcta cccaacccct
     3241 gagcaggccg aggaactggc ccggacgttc ggctgcgtgc gcctggttta caacaaggct
     3301 ctggaagagc gcacccgcgc ctatcggctg gaaggccgca agctctccta cgccgaatcc
     3361 tcggccgccc tcacggcctg gaagcgaacc gaggagctga gtttcctcaa cgaggtctcc
     3421 tgcgtgcctc tgcagcaggc attgcggcac cagcagatgg cgttctcgaa cttcttcgcc
     3481 agacgagccg gtcatccagt gttcaagtcg cgcaagaaat cccggtcgtc ggccgagtac
     3541 acccgatcgg cattccgctg gcgcgagggc aacctgacgt tggccaagat ggacggtccc
     3601 ctggacatcg tgtggtcgcg cccactgccc gagggcgcga agccgtccac ggtcacggtg
     3661 tcgagggatg cggcgggccg ctggttcgtg tccatcctgg tggaagagaa gatcgccccg
     3721 ttgtccccgg ctgggtcgat gatcggcgta gacgtcggga tctccgctct cgcgacgttg
     3781 tcgacaggcg aacggctcgc caacccgcga tatgaagcct cggacaggag gaaactcgcc
     3841 agagcgcagc gcgcgctcgc ccgcaagtcc cccggcagtg gcaatcgggc cagagcgaag
     3901 ctcgccgtcg cccggatcta tgccaggatc gccgaccgaa gacgggacta tctgcacaag
     3961 gtcaccacac ggcttgttcg cgagaaccaa gtgatcgccg ttgaagacct gaccgtcgcc
     4021 aatatggtca aaaaccgttc gctcgcccga gccatttctg atgcgagctg gcgggaactg
     4081 cgctccatgc tggcgtacaa agcgtcctgg tacggacggg aactgctggt cgttgatcgc
     4141 tggtttccct ccagcaagct gtgctcggcc tgcggggtgc tgaccgcctt gatgccgctc
     4201 gacgtccgag aatggtcgtg cggctgtggg gcggcccacg accgggatgt caacgccgcg
     4261 aagaacatcc tcgccgcagg gcttgcggag aggtgaaacg cctgtggagc tggcgtagga
     4321 ccccaaggag actcctctct ggcgggcggc cggccgtgaa gcaggaatcc tcacgggcga
     4381 ccgtgaggat cccctctcgt caaagagggg acgatgtcaa gcggctcctt gggtgcggat
     4441 cgcttcggcg agttcgtaga ggcccgcgag gtcggcgccg tcggccagca ggggcagttc
     4501 atatcgtggc accccggcgg tctccagcgc gtcgcgctcg gttatttcga gctcggcccg
     4561 ccgctgctgc tcggcaacct ctccgccgag ggcctcggcg atgaccgccg cgttgccgtc
     4621 gtcgcccagc ccggccgcct tcacgcccag cgccagttcg gccacgtcga aggggccttc
     4681 gaggacgggg tcgacggtgc gcaccatgtt gacgaagatc ccgccgacgg gcagcttggc
     4741 cgcccgcagc tcggtgacgc cgtcgagggt ctcctggacg ggcatctcct ccagcagggt
     4801 gacgaagtgg acggccgtct cgggcgaggc caccactccg cggaccatgt cggcgtgggc
     4861 gcgcaccggg ccgactctgg ccagccccgc cacctcctgg gtgacgttga ggaagcgggt
     4921 gacccggccg gtgggcgggg cgtccatgac gacggtgtcg tacaccctct tgccggttct
     4981 gtcgcgccgc cggaccgcct cgctggtctt gccggtgacc aggacgtcgc gcaggccggg
     5041 ggcgatggtg gtggcgaagt cgaccacgcc catcttggtc agcgccttgc ccatgcgctt
     5101 gaggccgtag aacatctcca ggtattcgag catggcctct tcggggtcga tcgccagggc
     5161 gtggacgtca ccgcctcccg gcgccaccgc gatcttgcgc tcctcgtagg ggagcggtgg
     5221 aagatcgaag acctgcgcga tgccctggcg gccctccacc tccacgagca gcaccttgcg
     5281 gcccccggcc gcgagggcca gggccagggc ggcggccacg gtcgtcttgc cggtgccgcc
     5341 cttgccggtc acgacatgga gacgtactcc gtcccagtcg gtgtcgcgag atctcacacc
     5401 cgtgaggcta cccaaacgtc atttcccgat acgccgaggc ctgctgggta tcgataacct
     5461 tccgccatga cgaagtggga gtacgtgacc gtgccgctgc tgacgcacgc gaccaagcag
     5521 atcctcgaca actggggcga cgacgggtgg gagctcgtgg ccgtcgtccc cggcccgaac
     5581 cccgagcagc tcgtggccta catgaagcgg gccaaggcgt gaccagcccc gaagaccgtc
     5641 tgtcggccct gggcctgtcg ctgcccgagg tggcggcgcc cctggcggcc tacgtgccgg
     5701 cggtccgttc gggctctcac gtctacacct cgggccagct gcccatcgcg gccggggagc
     5761 tgctgaagac gggcaaggtc ggcgcggagg tcacccccga ggaggcctac gaactggccc
     5821 gcgtgtgcgc gctgaacgcc atcgcggcgc tcaagtcggt cgtgggggat ctcggccagg
     5881 tcgccgggat cgtcaaggtc gtcgggttcg tcgcctccac gcctgacttc accggccagc
     5941 cgcaggtcgt gaacggcgcg agcgagctgc tcggcgaggt cttccagggc gcccacgccc
     6001 gcagcgcggt cggtgtggcc gtcctgcccc gggatgcccc ggtcgaggtg gaactgatcg
     6061 ccgaaatccg ttagatctac actcttacgg tggacattgc gctgccgggc gagctgggtg
     6121 aacgggcgcg tgacctgctg gccgggcggc tcgtcccggt tccggccagg gccgccgcca
     6181 cggtggccct gctgcgggag ccccctctgg aggtctacct gctccgccgc gtggccacga
     6241 tggccttcgc ggccggcgcc tacgtcttcc ccgggggctc cgtcgacccc cgggacgccg
     6301 gcccggacat cggctgggcc ggcccctcgc ccgccgagtg gggcgacctg ctgaacgccg
     6361 acgaggacac ggcgcggggc ctcgtctgcg cggcggtgcg cgagacgttc gaggagagcg
     6421 gcgtcctgct ggccggtgac ccggtcgacg acacgaccat gtgggaggcc gagcggctgg
     6481 cgctgatcag ccgggagctc tcgttcgccg agttcctggc cgccaacggc ctggtgctcc
     6541 gctccgacct gctcaagccg tgggcccact ggatcacccc ggagatcgag acccggcgtt
     6601 tcgacacccg cttcttcgtg gcggccctgc cgccgggcca gcgcaccagg gacgtgggcg
     6661 gcgaggccga cacggtcgtc tggacgagtc ccgccgacgc cctcacatcc ggatatttcc
     6721 tgatgccgcc gaccctccac acgctgtccg ccctggcggc ggccggaacg atcaccgacg
     6781 tgttcgccgc cgaccccaag atcaccaccg tcatgcccgc cccggtcgag cggaacgggc
     6841 agatggtcct gacgaccgag ggacagatct gatgagcggc ctctccctgc ccatagaggg
     6901 gcccatcgaa gggcccatcg acggctccgg caccgcgacc gccgtcaacg tgctggcccc
     6961 caacgcctcc ccgatgaccc tcgacgggac caacacctgg gtcctcggcc gaggcgagtc
     7021 ggtcgcggtg gtcgaccccg gccccgacga cccgggccac ctgtcgacgg tcgtgtcgcg
     7081 cctgggcggc cggaacgtgg aagtgatcct gctcacgcac ggccacgagg accacagcgg
     7141 cggagcccgc cggttcgccg agatggtgaa gtccccggta cgagccctcg accgcaccca
     7201 cgtcctgggc gaggagggcc tggaggacgg cgacgccctg acggccggcg gcgtggagat
     7261 ccgggtgatc ggaacgccgg gccactcgtt cgactcgctc tgcttctggc tccccgagga
     7321 cggcgccctg ctgacgggcg acacgatcct gggccgcggc acgacggtga tcgcccccga
     7381 cggcgacctg gccgactacc tccgcagcct cgaccggctc agggccatgg cggagctgtc
     7441 ggaggcggag tcgctgctgc cgggccacgg cccgatcctg gccgacccga tcggcaagct
     7501 ggacgagtac atcacccatc gcaggcagcg cctcgaccag atccgccagg cgatcaggga
     7561 cggcgcgaag gacgcgcgcg agatcgtgga gatggtctac gcggaggtgc ccagggaact
     7621 gtggccggcg gcggagatgt cggtcgcggc ccagctgaac tacctgaaga agctctagct
     7681 agcgcgagcg gttgtagagc cgctccatgt ccatgatcac gacggccttg gcctcgatgc
     7741 ggagccagcc tcgccccgcg aagtcggcca gcgccttgtt gaccgtctcc cgggacgccc
     7801 ccacgagctg ggccagctct tcctgggtca ggtcgtggtg gacccggatg ccgtcttcga
     7861 ccttctgccc gaaccgctcg gccaggtcga gcagggcctt ggccacccgc ccggggacgt
     7921 cggtgaagac caggtcggcc agcacgtcgt tggtccgccg gagccgctgg gccagcgccc
     7981 ggagcaggtg gagcgccacc tcgggacggc cggtcagcca gggtcgcagg tcgtcgtggg
     8041 acaggccggc gagcttcacg tcggtcagcg cgatggcgga ggccgtgcgg gggcgcgggt
     8101 cgaacagcga gagctcgccg aacatctcgg aggggccgag gaccgacagc aggttctccc
     8161 ggccgtcgga ggcggtgcgg gccagcttga tcttgccttc gaggacgacg tagagggtgg
     8221 ccccggtctc gttctcgctg aacagggtct gacccttcgt cagggtcacc tcggtgatgc
     8281 tggtgcggag ggccgcggcg ctctcacggt cgagcgcgga gaagaggggg gccctgctca
     8341 ggacttcttc ggtgttcatg cctgcctccc aacttcattc gcgatcgtag ccattgtggc
     8401 ctatgccccg ccttccgtat cacctggact gccagaaagc agacgtcgca cggctgcctc
     8461 gacgtaaccg gcgcgctcgt cgggggtgag ggtctcgacg gcggcggtgt agtggggagt
     8521 gaccgacgtc cacgcggagg cgagggagag cagcaggccg agcagcaccc cggcggggaa
     8581 cacggcgggc aggacgccct cggcctgcgc cttggcgatg gcctcgacct tggcccgggt
     8641 gctctccacg gcccgggggt tgggcgggtc gtccgcgcgc tccagtcgct gccaggtggc
     8701 caggcgggcg atctgcgggt gggcgtcgta gcccctggcc agggcggcgg cgtagccggg
     8761 gaggtcgtgg acgtcgatcg gggtctcccg gacgaccgtc tcgacgatgc cggtgaagac
     8821 ggcgtcgaac aactgatcct tgctgccgaa gtagtggtag atctgcgcct tgttcgtgcc
     8881 cgccaccgcc gcgatgcggt cgacccgggc gccggcgatg ccgtaggcgg cgaactcctc
     8941 catcgcggcg tcgagcagtc gctgtcgggt ggcgtcggct ttaccaggca tgccccaaga
     9001 ctattttcta accggttagt tgacaacttc cgagaccgct gcctagtgtt ctaactaacc
     9061 agttagtcag aaggagtcga acatgatctg gttcgtcacc ggcagctccc gcggtctcgg
     9121 ccgcgagatc gtcctggccg cgctcgaacg cggcgacagc gtcgcggcga ccgcccgcac
     9181 gcccgcccag ctcgacgacc tctcgcagaa gtacggcgac cgcgtcctcg cgctccccct
     9241 cgacgtcacc gacgccgaag cggtcgaaga cgccgtggcc acggccgtgg aggcgttcgg
     9301 cacgctcgac gtcgtggtca acaacgccgg ctgggccgac atgtcggcct tcgaggacac
     9361 ccccatcgac aacttccggg cgcaggtcga gaccaacttc ttcggcgtcg tcaacgtgac
     9421 caggtcggtc gcgccgatcc tgcgccggca ggggcacggc tcgctgatca acgtctcctc
     9481 gatcggcggc cgtctcggca cgcccggcct gtccgcctac caggcctcaa aatgggccgt
     9541 gaacggcttc accgaggtca tcgcccagga gctcggcccg ctcggcgtcc gggtcaccac
     9601 gatcgagcca ggcggcatga ccaccgactg gggcggcgcg tcgatgacga tcccgccgat
     9661 ctccgagccc tacgagcaga gcgtcggggc catggtgcgg ctgctgcggc agggcgagcg
     9721 caagccgctc ggcgaccccg ccaaggtcgc ccaggtgatc gtccggatca ccgagatgga
     9781 cgagccgccg gtccggctgc tgctcggttc cgacgccctc aacggcgccc ggtacgccgc
     9841 ccacaccacc gccgagcgcg acgcggcgta cacggatctg agccgttcca ccgaccgtga
     9901 cgacgccacc gcccaggagc gtgatcctct gggtcaagca ttctccacga gcgccgatgg
     9961 cgccgcggtg gagtgaggcg ggtcgtgtat cggtagccgg gtgaccgact cgatgaccat
    10021 ccgggacgtc ctcaactcga cccgcgaact gatcgaaccc gcccaccggg ccgcgatcga
    10081 ccggctctcc ccggagctgc ggcacgtcgc gggctaccac atcggctggt gggacgacga
    10141 gggcaacgag ctcatcggcg gccagggacg gttcgtgcgg cccgcgctgg cgctggggtg
    10201 cgcgcgggcg gccggggggc acgccgccga cgcggtgccg atggcggtcg cgaccgagct
    10261 ggtcaacgac ttctccctgc tccacgacga cgtgatcgac ggcgacgaca tcaggcggga
    10321 gcgcccggcg gcctggaccg tcttcgggct cagcgaggcg ctgctgaccg gcgacgccct
    10381 gctggcggtc gcgatggcgc tggtcggccc ggccggggtg cggaccctcg ccgacgcgct
    10441 gctcgacctg tgccacgggc aggccagcga cctggccttc gtcgaccggc cccaggtcac
    10501 cctggccgag tgcatgacca tggccgagaa caagaccggc gcgctgctcg gcgcggcctg
    10561 cgagctgggc gcgctggccg ggggagccgg tgacctccag gtcttccggg agttcggcac
    10621 ccgcctcggg ctggccttcc ggctcactga cgacgtgcgg aagctggtcg ccgacctggg
    10681 ctgccgccgc aagtcgctgc cggtcgtcgc cgccctggaa tcgggtacgc cccccgccgc
    10741 ccgcctggcc gagtcactgc gcgacggggg cgaccccgag acgcaggccg acctggtcga
    10801 gcggacgggc gcccgcgaat gggcgctccg ggaggccgac cgtcaggtcg agctcgcttt
    10861 cacggcgctc ggccacgggg ccaccccgga gctgcggctg ctggccgacc tgagtctcgg
    10921 ccggactcgc tagtggatgc cggtgcggac cgaggtgatc cgcccggtgt tgaagccgag
    10981 ccagtgcatg cggcccatgt agcgggcgtg ctcgatcttc atgcagcggt ccatgatcac
    11041 cgtgaggccg ccctcctcgg cgatgcgggc gccctcctcg ctgatcacgc cgaactggag
    11101 ccacagcgcg cccgcgccga tggcgaccgc ctcacgcgcg atgccgggga cggcgtcggg
    11161 cgcgcggaag acgttcacca cgtcgacctt gacggggatg tccgagaggc tcgggtagga
    11221 ggtctcgccg aggatctcct tctcgcgcgg gttgaccggg atcatccggt agccgtggcg
    11281 cttgaggtag aagccgacga agttgctggc ccgcagctcg ttgcccgaca ggccgacgac
    11341 cgcgacggtc ttggcggtgt tgacgatccg ctggatgtcc tgtggttcct gatatctcat
    11401 gccttctcca cgtgcgcgaa gccctgctcc aggtcccaga tcaggtccgc caccgactcg
    11461 acgccgaccg acaggcggat ggtgccggcc ccgaccccgg cggcggccag ttcggcgtcg
    11521 ccgagctgac ggtgggtggt gctggccggg tggatgatca ggctcttggc gtcgccgacg
    11581 ttggccaggt gggaccagag cgtcgtgccg cggatgaagg cctggccgcc ctcacggccg
    11641 ccggcgcact cgaaggagaa gaccgcgccc gcgccgcgcg gcaggtactt ctccaccagc
    11701 gccttgtacg agctgtcggc cagccccggg taggtcacct tcgaggccag ctcgtgggcc
    11761 tggaggaact ccgcgaccgc gagggcgttg tcgacgtggc ggtccatccg cagcgacagc
    11821 gtctccaggc cctggaggaa caggaacgcg ttgaacggcg acatcgccgc gcccaggtcg
    11881 cggagggtct cggcgcgcag cttcatcagg tagccgtatt cgccgaacgt ctcgtggaac
    11941 ttcagcccgt ggtaggccgg agacggctgg gcgatcaccg ggaagcggcc gttggagtag
    12001 tcgaacgtgc ccgcctcgac gacgacgccg ccgatgctgg tgccgtggcc gccgatgaac
    12061 ttggtcgcgg agtggacgac gatgtcggcg ccccactcga tcggccggca cagatagggc
    12121 gtcgcgaacg tgttgtcgac gatcagcggc aggccgttgt cgtgggcgat ggccgcgacg
    12181 gtctcgatgt cgagcacgtt gccggccggg ttgccgatcg tctcgccgta gaacgccttg
    12241 gtgttcggct ggaccgcggc gcgccaggcc tcggggtcgt cggggtcgac ccaggtcagc
    12301 tcgacgttca tcttgcggaa caggtgcttg agctggttga ccgtgccgcc gtagagggcc
    12361 gaggaggaga ccacgtggtc gccgggctcc agcagcgtga acagcgacgc ggcctgggcg
    12421 gcgatgccgc tggcgaacgc caccgcgccg gtgccgccct ccaggttggc catccgctcc
    12481 tcgaacaccg ccgtggtcgg gttcatgatg cgggagtagg tgttgccgta ctcctggagg
    12541 ttgaagtagg ccgccgccga ctcggggtcc tcgaagacgt aactggtggt ctggaagatg
    12601 ggcaccgcgc gggcgccggt gttcgggtcg ggccgctgtc ccgcgtggag ctggcgggtc
    12661 tcgaacccga actcccgggc ttcttcggta cggaatggtc tgtcgctcac gaagccatct
    12721 tcctcgtcaa gaactgccgg atgagggggg tctgccgtgc ttcctccagc aggaagcagt
    12781 catgaccgta cggtgcgtcg atgaggtggt tctccaccgg tttgcccaga ttagtgaggg
    12841 cggcggcgat ctcgtccgac gcctccggcg gatagagcca gtcggagctg aacgagatga
    12901 gcagcgtccg ggcctggacg ttctccagcg ccttctccag cgacccgccg ccgtgctccc
    12961 tggccaggtc gaagtaggtc agcgcgcggg agatgtagag gtagctgttg gcgtcgaacc
    13021 gcttcacgaa cgagtcggcc tggtggcgca ggtagctctc gacctcgaac tcgggctcga
    13081 cgatcgtgtg gcggatgtcg tcggagaact ggagccgccg gccgaacttg tcggtcaggg
    13141 ccacgccgga gagataggtc acatggccga ccatgcgggc cacgcccatg ccggcgtcgg
    13201 gcttgcggcc ggtgccgtag tagtggccgc cctgccagtc ggggtcgcgc atgatggact
    13261 cgcgggcgat cgcgttccac gccagaccct gggggtggag ggcgtgggtg gaggcgatga
    13321 ccactatgga gtcgacctga cccggatact ggaccgccca ttcgagggcc tgcatcccgc
    13381 ccagcgaacc gcccgcgatc accgcgagac gctcgattcc gagctcgtcc aagaaggcgc
    13441 gttcggtgcg gaccatatcc gccacggtga ccaccgggaa gtcggagcca taggggcgcc
    13501 cggtggcgga atcaatggtg gaagggcctg tcgtgccgct gcagccgcca aggaggttcg
    13561 tgcagacgac gaagtatcgg tcggtgtcga aagcctttcc gggcccgatc atcccgtccc
    13621 accagcccag ccctttcccc ttcacgccgt cgcgatcctc ggcgccgaag ccgtcgcggg
    13681 cgctcgcttc gggggccgtc ttcgcggaac ctgccgcatg ggcgttgccg ctgagggcgt
    13741 ggcagacgag tatgacgttg tcgcgctcgg gggagagtgt gccgtaggtc tcgtaggcca
    13801 cacggacgtc gcgcagttcc cgaccgctgt cgaggcgtat cggaccaggc aggtcgaggt
    13861 attgagtttc aacgaaacct atggagccca tgggtttcat tgtattgttc atttaacctg
    13921 taggaaatga ggggatcatc atgacgaacg gagtgaacgt ccaggccctg ctggacgccc
    13981 gcgaggttct caagggcgcg cccgaggcgg cccagttcac ctggcgcgcc agctccaagt
    14041 ggcagcacgg cgtccacagc accaccagga tcgagaagtt cttcggcctc ggccaggagc
    14101 agagccacaa gagcgagtcg gtcttcgagg ccgaccaccc cgccatcttc gcggccgagg
    14161 acaacggcat cacccccatc gagtacctgc tcgtgggcct ggcctcctgc ctgaccgccg
    14221 gcatcgcctc ggtcgcgcag aaccgcggca tcaagctcaa ctcggtcgag gcctccgtcg
    14281 agggcggcca cgacatccgc ggcatcctcg gtgccgacag cgacgtgcgc aacggcttca
    14341 acgacatcaa ggtgaccttc tccatcgacg ccgacgcccc ccgcgccgac atcgaggctc
    14401 tcgtcgccca gtcgcagaag cgttcggccg tcttcgacgc gctgaccaac ccgacgaacg
    14461 tcaccgtcga agtcgcctga tcgtgaacgt caccacggcc gtcatcggcg caggtcacgc
    14521 gggcctggcg gccagccact tcctgacggc cgcgtcgatc gaccacgtgg tgctggagcg
    14581 cggcgaggtc gccaactcgt ggcggcacga acgatgggac tccctccggc tcctcacccc
    14641 caactggctc aaccgtctcc cgggctccca ctacgagggc cccgaccccg acggctacgc
    14701 cgacatgggc gaggtggtcg attttctgga gcgttccgcc aaggacgtcc cggtccgcac
    14761 cggcgtgacg gtcacgtcgg tgcggcgctc ggccggcgga tacacgctga cgaccgacca
    14821 ggggcccgtc gaggcccgga ccgtcgtcat cgccagcggc gcctgcaacg tcgcctcggt
    14881 gccggcgctc gccgcggcgg tgccctcgtc ggtggagcag gtgacgccgt tcggctaccg
    14941 gaacccctcc cggttgccgg acggcggcgt gctcgtcgtc ggggcctcgg ccacgggggt
    15001 gcagctcgcg gccgagatcc gccgctccgg ccgcccggtc gtgctgtcgg tcggcgagca
    15061 cgtccggatg ccccgcgtct acgagggccg cgacgtgctc tggtggatgc acaactccgg
    15121 cgtctggagt cagcgctacg acgaggtcga cgacctgacc cgggcccggc ggctgccgtc
    15181 gccgcagctc gccggggcgt ccaccctgga cctcaacgtg ctgaccgcgc tcggcgtcga
    15241 actggtcggg cggctctcgg cgatccgcga cggcgcggcc ctcttctccg ggggcctgcg
    15301 caacgtggcg gcgctggccg acctcaagat gaaccgcctg ctcgacacgt tcgaccagtg
    15361 ggccggaacc gcgccggggg agcgcttcgc gcccaccgac gtgcccggta cggcccgctg
    15421 gcgggtcgac ctggggtcgg gggagatccg ctcgatcgtc tgggcgaccg gcttccggcc
    15481 cgactactcg tggctggacg tcccggtcgt cgacccgaag ggcgcgctga cccacgacgg
    15541 cggcgtgctc ggcgacggcc tctacgccct cggcctgccg gtcctgcgcc gcagacggtc
    15601 gacgttcatc tgcggcatcg aggacgacgc ccgcgaggtc gtcgaccacc tgcggctgca
    15661 cctagccggt tagtcccgcc agaacatcag ggagtcgacg aactcccaga acggacgatc
    15721 ggtcggctcg gccgcgacga cgagccgcac cgtgtccggg gcgtggccgc cgaacacccg
    15781 ctgtacgtgc cacgacccgg gcaccttctc cggctggacg gtgtgggcgg agtagtccca
    15841 gccgccgacg tgatccggcg gcctctccgg gtggtaggac tggacggtga cgttcagctc
    15901 ccgcagcaga cgggcggcct cgtcgaccgt gtagttcgtc aggtcgaatc cctccagggg
    15961 ctcaccgggc gccgtcggat cgccgccgac ctcgtagcgc tccccgggct caggcgcccg
    16021 gccgatcgtg agttccagag ggctgcgcag atcgaccggc acactgaccg cggtgacgca
    16081 ggagcggtcc ccgcagcggg ttcctcggat cgaggtgatc cactcgctgc cctgttcgcc
    16141 gacgatctcg aacccgtcgg tatggtagtg gaccatccgc tggggagaat tgacggagat
    16201 caccgcgccg accaggctcg gtgaggtggg caccgtgtcc agcgtgacgt ccaggccgtg
    16261 cgcggcgaag tccgcgcgga agcgctcagg atcggcgttg gggtccgcga tccggatgtc
    16321 gatgcgatcg ctgttccggg agaacgcgac ggatttggcc gggcccagtt cgtaggaggc
    16381 gctcacgccg ggctgcgaca tgatcaccag gatcgccgtc agagcggtga gggggatcag
    16441 gaccagagac ctccgccggc ggcgcctcac cggccgtggc gttctgacga tggtgtccag
    16501 gacggcggcc atcgtcgccg ggtctgcccg cacgtggcag gccgggtcgt tgtcccgcaa
    16561 ccagttcatc ggacgctcct ttccggttcc tcgacgaaca ggtgccgcag ccgcctgcgt
    16621 aatcggtgca cgcgcatcgc cgccgtcgcc cgggagcagc ccacgacctt cgctgcctgg
    16681 ccgaggtcga gcccctccca cgacatgagc aggaccagtt cgcggtcgcc ctcgctcagg
    16741 ctgttcagcg cggccagcgc cgcctggcgg tcggtcaccg ccgccgcgtg atcctcttcg
    16801 gcgccgcccg cccactcggt cagcgcgtcg cggcggtggg tgcggcggat caagttcaag
    16861 gtgaggttgc gggcggtcgc gtagagccag gggagcggct ggtcgcgggg cacccggtcg
    16921 tacttctccc acgcgatctt gaacgtctcg gcggcgatgt ccgcggcgtc ggcggggccg
    16981 gcccggcgcc aggcgtagcg caggaccggc gtgtagtgct ccttgaacat gcgcgtgaag
    17041 tcggcctcat cgctcactcc gtctcctctt cagtgctgcc tctaccggta gatgtcgcgg
    17101 agcgcgcggt tatcacccgg gagacatgcg aagagcgggc cggggaacga ccacggcccg
    17161 ctcggtgcct gaacgcgtcg gcgactcgac cttcggtcag gggagatcgg ttccggtcac
    17221 ctcacgcggt gcgggccgcg tgaggtgact cctagacgac gggcgtgcgg gtgagctccc
    17281 acttgatggt gatgacggtc cggcccgact tgccgtgcgt gtcgatgcgc agcgacttgc
    17341 cggggacgca ggtgtaggtg cccttgcggg ggatcgcccc gaggtcctcg ttgcgtgcga
    17401 ccgcgtcgcg gagcaggccg gagaagccct ttccgccgcc ggagcgctcc aatctggcgc
    17461 cgatgttgcc ggtgcggggc ttgagggcga ccttgccgta ggtctggggt ccgtggccgc
    17521 cgaaggtgta cgtgaagttc tcacggccct tcagcctgaa ggtggcgccg ccggtcagca
    17581 gggccgcggt gcggaagtcg tagtgggcgc cgacgtactt gccctcgggg gtgaaggtcg
    17641 ccttgatgcc ggcgcctccc tgcgcgaaga agttgtgctt cttgccggcg acctcggcgg
    17701 tcgccgtggt caccgtccac gaccccgccc agcacggcgc cttgggcgtg gaggtggggg
    17761 tgggggtggg ag
//
