LOCUS       NZ_BBXG01000009        21140 bp    DNA     linear   CON 08-AUG-2024
DEFINITION  Herbidospora cretacea strain NBRC 15474, whole genome shotgun
            sequence.
ACCESSION   NZ_BBXG01000009
VERSION     NZ_BBXG01000009.1
KEYWORDS    WGS; HIGH_QUALITY_DRAFT; RefSeq.
SOURCE      Herbidospora cretacea
  ORGANISM  Herbidospora cretacea
            Bacteria; Actinomycetota; Actinomycetes; Streptosporangiales;
            Streptosporangiaceae; Herbidospora.
REFERENCE   1
  AUTHORS   Komaki,H., Ichikawa,N., Oguchi,A., Hamada,M., Tamura,T. and
            Fujita,N.
  TITLE     Genome-based analysis of non-ribosomal peptide synthetase and type-I
            polyketide synthase gene clusters in all type strains of the genus
            Herbidospora
  JOURNAL   BMC Res Notes 8, 548 (2015)
   PUBMED   26452464
  REMARK    DOI:10.1186/s13104-015-1526-9 Publication Status: Online-Only
REFERENCE   2  (bases 1 to 396994)
  AUTHORS   Hosoyama,A., Oguchi,A., Ichikawa,N., Kimura,A., Ohshita,R. and
            Fujita,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAY-2015) Contact:Director-General Department Of
            Biotechnology National Institute of Technology and Evaluation,
            Biological Resource Center (NBRC); 2-49-10 Nishihara, Shibuya-ku,
            Tokyo 151-0066, Japan URL :http://www.nite.go.jp/nbrc/index.html
COMMENT     ##Genome-Assembly-Data-START##
            Finishing Goal                    :: Noncontiguous Finished
            Current Finishing Status          :: High-Quality Draft
            Assembly Method                   :: newbler v. 2.6
            Genome Coverage                   :: 71x
            Sequencing Technology             :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_001570605.1-RS_2024_08_08
            Annotation Date                   :: 08/08/2024 02:39:29
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,566
            CDSs (total)                      :: 7,487
            Genes (coding)                    :: 7,430
            CDSs (with protein)               :: 7,430
            Genes (RNA)                       :: 79
            rRNAs                             :: 4, 5 (5S, 16S)
            partial rRNAs                     :: 4, 5 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 10 of 57
            Pseudo Genes (incomplete)         :: 49 of 57
            Pseudo Genes (internal stop)      :: 3 of 57
            Pseudo Genes (multiple problems)  :: 4 of 57
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 12:10:14
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 44060
            Orig. end                         :: 65200
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            BBXG01000009.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..21140
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="darobactin"
                     /region_number="1"
                     /rules="((cds(PF04055 and (TIGR04085 or SPASM)) and
                     darobactin_precursor) or darobactin_rSAM)"
                     /tool="antismash"
     cand_cluster    1..21140
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="((cds(PF04055 and (TIGR04085 or SPASM))
                     and darobactin_precursor) or darobactin_rSAM)"
                     /kind="single"
                     /product="darobactin"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..21140
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[10000:11140](-)"
                     /cutoff="5000"
                     /detection_rule="((cds(PF04055 and (TIGR04085 or SPASM))
                     and darobactin_precursor) or darobactin_rSAM)"
                     /neighbourhood="10000"
                     /product="darobactin"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(10001..11140)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="5000"
                     /detection_rule="((cds(PF04055 and (TIGR04085 or SPASM))
                     and darobactin_precursor) or darobactin_rSAM)"
                     /neighbourhood="10000"
                     /product="darobactin"
                     /protocluster_number="1"
     misc_feature    complement(1239..1259)
                     /note="TFBS match to NrtR, NAD synthesis repressor,
                     confidence: weak, score: 22.12"
                     /tool="antismash"
     misc_feature    1370..1383
                     /note="TFBS match to ANR, Anaerobic transcriptional
                     regulator, confidence: medium, score: 21.93"
                     /tool="antismash"
     gene            2835..3212
                     /locus_tag="AW270_RS19255"
     CDS             2835..3212
                     /GO_function="GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /locus_tag="AW270_RS19255"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="FAD-dependent monooxygenase"
                     /protein_id="WP_218009282.1"
                     /transl_table=11
                     /translation="MQRARAPSRAVRGEPSAARSPPGEERVLPLRSVVYSPMSRGKLYL
                     LGDAAHIVPPMSAKGINLALNDAEVFARAAIQQIQQGDSRLLDSYSDTCLSRIWNYQAF
                     AAWITEIMHNVGDTSYAGEFR"
     gene            complement(3634..4170)
                     /locus_tag="AW270_RS19260"
     CDS             complement(3634..4170)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="AW270_RS19260"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_061296257.1"
                     /transl_table=11
                     /translation="MRQVVEPVYGDERLPSLCKHYDILDKLVLHDDPSGFRVRLHLFGP
                     DHYDRPHNHRWTYSARLLSGRHIPSLYGTEDDFDNTIDVAALQPLMVRTEQPGPGYTPH
                     HSMVHAAVSRPDTVSLIVRGPAVKDRFLVTDRTTGQVWWQYGAAPESAAEAAAKRMPPA
                     MINDCVARLEKLAIV"
     gene            complement(4218..5399)
                     /locus_tag="AW270_RS19265"
     CDS             complement(4218..5399)
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF017885.4"
                     /locus_tag="AW270_RS19265"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="WP_174549874.1"
                     /transl_table=11
                     /translation="MSSSKQGRRISGQSGLDESSSPQAILDAFDPARLTQARHLAGMTK
                     KELADSIDVSPAAIGQYETGTNRPRWDLVPRLADVLGVPMQFFLAGRPHGKLDGSMTYF
                     RSLRSARVYQRAKAMALVEQVWELALALEKQVTFPPVDLPGFSGGEVHPGTELSRDPVR
                     AAQALRQRWGLGTGPISHLVRHLEAHGIIVVLPPADKDVATVDAFSTLQLPRPIIVLTA
                     NRYDDIHRYRYTAAHELGHLILHGDAPAGDSRQEREADSFAAEFLTPRDSILSELPGRL
                     DLRRLAELRHKWGVSVDSLLYRCREVGLLSDSAASRGYQRLHELRSQPGFTSESITGYP
                     GELPVLLRQAFELATEQTGLTMHDLAGQLAWPASRVRELLGIADQRPVLRIVP"
     gene            complement(5389..6180)
                     /locus_tag="AW270_RS19270"
     CDS             complement(5389..6180)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="AW270_RS19270"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_061296259.1"
                     /transl_table=11
                     /translation="MSGDESSNWATRRFGEQAGAIRDLVSKSFLQAVANAQDAQEVSGS
                     KKRFTFGSTLMTLRYERLVENFRDLPEVQIVKPKAAPYELVLVGENLLFPFRYAKDNTT
                     PIEDARIGESRLSGLVESLFNQFGPKPSYVQDPIWDTPEDTGPEPVLTHLPEGTRLILI
                     AHAANDKAGPIQLYWGEAVVVDAFGRLKWIDKELIQLTRPDGRLRSVATPELESTERFD
                     HGTLPDLTITPRPEIERKNEAVFPVNSEADEETASAEDHEQ"
     gene            complement(6542..6781)
                     /locus_tag="AW270_RS19275"
     CDS             complement(6542..6781)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="AW270_RS19275"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_061296261.1"
                     /transl_table=11
                     /translation="MARLLGIGWYGPWIAYAAESAPPGHTSFALGLAMAINQVAVILAP
                     TVLGLLTDLTGGFTAPWVPLAALTAVTLLLTSRP"
     misc_feature    complement(7212..7227)
                     /note="TFBS match to CRP, Cyclic AMP receptor protein,
                     confidence: strong, score: 19.26"
                     /tool="antismash"
     misc_feature    7826..7844
                     /note="TFBS match to HgtR, Hydrogen-dependent regulator,
                     confidence: weak, score: 20.89"
                     /tool="antismash"
     gene            complement(8005..8658)
                     /locus_tag="AW270_RS19280"
     CDS             complement(8005..8658)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="AW270_RS19280"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_061296263.1"
                     /transl_table=11
                     /translation="MTLIDSYTRALRELDWNDAAQVEAATGELLSALAQDTTALRQAVE
                     AVSGDEHLQSLCEHYDILDKLVLHDDPSGFRVRLHLFGPDHYDRPHNHRWTYSARLLSG
                     SYTHTLYGTEDGFDDTTDVTALQPLMVRTEQPGPGYTLHHSMVHAAVARPATISLIVRG
                     PAVKDRFLVTDRATGKVWWQYGAATESAAEAAVKRMTPVTINDCIARLEKLALI"
     gene            complement(8669..9544)
                     /locus_tag="AW270_RS19285"
     CDS             complement(8669..9544)
                     /EC_number="2.7.1.-"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0016301 - kinase activity [Evidence IEA]"
                     /GO_function="GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA]"
                     /GO_process="GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1276: PfkB domain protein"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /locus_tag="AW270_RS19285"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="carbohydrate kinase family protein"
                     /protein_id="WP_061296265.1"
                     /transl_table=11
                     /translation="MTSIVDLSCFSYLAAARLLTVDRYPAADTGAEVHDALQSLAGDGP
                     ITAITAASQGLRVALSGNDVGEDAVGLDLLAQLERYGVRHRISARPGTVTPNLTVITDR
                     TGTRTWFAHLSSATADLLDADMSLLAPARLAYIDCYEIITDAAVQALEHVTRSDGPIML
                     NLGGDPIHPAIQKAAVGTNLLAVQTSLPDNQVNAAERAADSVYEQLRPQITLVTLGRHG
                     VLARTETGFHHEPACTGPIAHTHGAGAAFSAGFAVAHLAGSDIKDCLIHACRTGTEHCS
                     PEPSPLSLPH"
     gene            complement(9546..9983)
                     /locus_tag="AW270_RS19290"
     CDS             complement(9546..9983)
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017536402.1"
                     /locus_tag="AW270_RS19290"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="NUDIX domain-containing protein"
                     /protein_id="WP_061296268.1"
                     /transl_table=11
                     /translation="MSSERYKTCVDLHLILRRGDQVLLGQRQNTGYADGSWHLPSGHLD
                     PGESASQGALREAAEEIGVTIEPGDVQFAHLMHHYTNDARTALFYEAEQWTGEITNMEP
                     DKCVEWRWFPLDALPDQMIPYAADALAHCIKGDVYSERGWS"
     gene            complement(10001..11140)
                     /locus_tag="AW270_RS19295"
     CDS             complement(10001..11140)
                     /GO_function="GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /GO_function="GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     darobactin: TIGR04085"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     darobactin: PF04055"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     darobactin: darobactin_rSAM"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PTHR43273"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Fer4_12"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /locus_tag="AW270_RS19295"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="radical SAM protein"
                     /protein_id="WP_061296270.1"
                     /sec_met_domain="PTHR43273 (E-value: 5e-33, bitscore:
                     109.1, seeds: 35, tool: rule-based-clusters)"
                     /sec_met_domain="Fer4_12 (E-value: 3e-06, bitscore: 22.1,
                     seeds: 84, tool: rule-based-clusters)"
                     /sec_met_domain="PF04055 (E-value: 1.4e-18, bitscore: 62.4,
                     seeds: 518, tool: rule-based-clusters)"
                     /sec_met_domain="TIGR04085 (E-value: 1.4e-09, bitscore:
                     32.8, seeds: 281, tool: rule-based-clusters)"
                     /sec_met_domain="darobactin_rSAM (E-value: 1.0, bitscore:
                     1.0, seeds: 0, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTSVDLPMPAIRTGSDAYGHARMPVSADDPEVRRIDRVPYGRFRN
                     VYLYITEACQLRCGSCYMGQRLERALKMPFEEIRNNLTAWRQMGGSKLTILGGEPTLHP
                     RYEDTIRLAGELGYEHVITTSNGLGPAARKFRRLEPSDFAYVQISMDGGSPATHDAVRG
                     EGTFATTLETIRELTGRGFDTRIICTVNKANQSDCLNLLTIADDLGVSLVKFHVFSVIG
                     RGHDNESWGMNPTEWLQFCDELPLIAAKHRTRVWYQPTFARRSEMPRYEEEGYRGCIGR
                     TLDRISIFPDGRAYVCSFLFDTELNYATSTNGQIEINKGPNEFDLFTGALTKAACGDCK
                     ATTSCMGGCPAEELIDGAASCTEPDIVPVCRLWKSTPAN"
     gene            complement(11137..11823)
                     /locus_tag="AW270_RS19300"
     CDS             complement(11137..11823)
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF012624.4"
                     /locus_tag="AW270_RS19300"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="nucleoside monophosphate kinase"
                     /protein_id="WP_061296272.1"
                     /transl_table=11
                     /translation="MTGPVVILFGPPASGKSTLSEQLVAKRNARLFRLREYVQSLPEAE
                     TAVLKGTSRDSLGWLPDDIATQLVHDAISGPFQPGETPVVFEGYPGNGVQSRHLADLLT
                     RLDVSATVIHLRADWATALQRAQLRRVCLHCEPTATGQPHKPAQVTTADTCARCGNTLL
                     QRQDDNPDRLSSRTQRFTSRLPEIYTALTGHGIPWRTVDSHPPACEVLAVALDAVSNCP
                     STRSSQ"
     misc_feature    complement(11722..11724)
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            complement(11820..12758)
                     /locus_tag="AW270_RS37845"
     CDS             complement(11820..12758)
                     /GO_function="GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /locus_tag="AW270_RS37845"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="WP_082801670.1"
                     /transl_table=11
                     /translation="MTPPISSVVYLPGTALDEKLADLGYAAMRGWPDQRPVTSALVRSR
                     LRRNGNAQPTTMLLARLPNGTLAGAAALRHGTAPGLSARLWGPIVAPEWQRRGLGTALL
                     QQAVRCHDGGASLLTAEIPEARKHGCEFFERAGWQLHSTATLLKADLTALPDVPHPGRV
                     GVRQAEASDADALSRLYQSIHPEHGQQVAEDTYLRWTADERFVSDGLLIAAGTGDQLRG
                     AALMYPLAHMDPAEPTEALLADVLIDPATERSDVAAPLIAAALAAGLRHHAPVVRAIVP
                     DSATDLLSDLHAVGLAPAGQIRYFRANGRVS"
     gene            complement(12755..13561)
                     /locus_tag="AW270_RS19310"
     CDS             complement(12755..13561)
                     /EC_number="2.1.-.-"
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="AW270_RS19310"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="WP_061296276.1"
                     /transl_table=11
                     /translation="MTGSLTIRGAWTAARENCARGCFCYHASWRLFRRTGLKGNPRWHS
                     AFYQRALAGRQPSEALVCGSSDETMPQVLTELQPSMRITVADSCPTPLVLINAWAKETG
                     VQVAALQSEAPELCGIAGPFDLVVTDGLLSLLPGPADRQAVLARLAGLLSDDGLLLYTT
                     RVAGLAGHLEYDRIGRVGQALTATTWPATSAERMDLARQRLRRPSRPSPFASQNQIASV
                     FRSRFGQVRVFTRSAPPTAALALHPAFLTGRGSVCVGIAATRPRRP"
     gene            complement(13558..14334)
                     /locus_tag="AW270_RS19315"
     CDS             complement(13558..14334)
                     /GO_function="GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1089: methyltransferase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /locus_tag="AW270_RS19315"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="methyltransferase domain-containing protein"
                     /protein_id="WP_061296278.1"
                     /transl_table=11
                     /translation="MSNSWTSASGADLSAGYARHAGTLRGALRHALVARALRIHLPADP
                     QQVLDVGGGDGHQAVLLARAGHRVTLLDPDPGMHKRATDRLTAESDQVRTRVMLVEGTA
                     DQARDLAENGFDLVCCHGVLMYAEDPHTFLTDLASAVRPGGLLSLLTKNADALAMRPGL
                     EGRWADAAAVMGAGSVEGNLGVSSRADTLAAVETILGDAGVTTSEWYGVRVFTDHLGDA
                     PVGEGFDYVLEAEWQAGCRDPYRQVARLFHLIGRKQ"
     gene            complement(14331..14969)
                     /locus_tag="AW270_RS39880"
     CDS             complement(14331..14969)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="AW270_RS39880"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_061296282.1"
                     /transl_table=11
                     /translation="MITAQGERLFTAQDAGLLLSPGVVTCGARRRVWHEEHDPLAAVRL
                     ALTSAQRSWVEAVAIAPTGIALAQTGLALAFSRHLSQIRGRRSGEAACVTSPVPPPLPK
                     SRLCRIPHLVTAAGPDGVWGDAVVWEVMTVERLRSWLDRDLPIDLQQVEARIPQLIALR
                     RAAREGTLPDTPGVRVLRELLRTRYLSIRLVLQHQDLFDSLLSPKETHP"
     gene            complement(14966..16450)
                     /locus_tag="AW270_RS19325"
     CDS             complement(14966..16450)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="AW270_RS19325"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_061296284.1"
                     /transl_table=11
                     /translation="MESVVTTEIRGEAADLLMVRNAVIEAIAKVTDIGEATHDGAGTRL
                     RLGTEACVRLFVSTPSRPKATSSDTHIATLIMEETEGLSPDQVQTLQTAVRRIPFTTTE
                     MKALRGQMPLTGELPAFLPPGTLSSLAPVLTVHHMTDFLVMVESVLAMGVPAQAITVLD
                     KGYPYQHTDRVDAHLTRQGIAVWPWTNATDALDDHTRRAAALGLRGLLIDDGGYTLPVL
                     LAQRPDLLPHFIGLVEQTISGITRLEPWEGRLPLPIFSVAESRLKATVECYGIADAAVR
                     NLLRLLPEEKLEGRPALVVGFGRIGEHIADVLRSRRMRVAVHDRQLVRLIAAHERGYLT
                     NRCLSTLLRDHRPLLVIGCTGRTGLRGEHAAAIGRNCYLVSTTSRNREFAVAELEEEAR
                     LVTDEGVLGTRLHLPSGVQVTLVGDGFPINFHHAESLPNMYSDLILASLLVAASTLAAP
                     DHGFIAGHNVEASNRVLESCGLLERYYARFGPEATR"
     misc_feature    complement(15356..15358)
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            complement(16768..17082)
                     /locus_tag="AW270_RS19330"
     CDS             complement(16768..17082)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="AW270_RS19330"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_157522241.1"
                     /transl_table=11
                     /translation="MMVLRRAIDRFKHPKGDHHPGSSRTARRDRRIDHYAGHRFADELN
                     REHSGWLVIWLTWQREFCAMAQWPVDTPLVVTAPTPKELRRAVLNAEMSLIRTGVLPPR
                     "
     gene            complement(17079..17294)
                     /locus_tag="AW270_RS19335"
     CDS             complement(17079..17294)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) darobactin_precursor"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020543727.1"
                     /locus_tag="AW270_RS19335"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_061296288.1"
                     /sec_met_domain="darobactin_precursor (E-value: 1.0,
                     bitscore: 7.0, seeds: 0, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MPSPTPEVPDTWRYLTSDVGRLWAWRKEDFGKTAREAGATPAVDA
                     DDLAALARAINSQEQIAQRADEEYQS"
     misc_feature    complement(17595..17608)
                     /note="TFBS match to ArgR, Regulator of arginine
                     biosynthesis genes, confidence: weak, score: 17.46"
                     /tool="antismash"
     gene            complement(17882..18067)
                     /locus_tag="AW270_RS19340"
     CDS             complement(17882..18067)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="AW270_RS19340"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_061296291.1"
                     /transl_table=11
                     /translation="MIAWTCACWPIVYEKLMVGGSYLIRHTNQSDGSGAVPLESPPTRH
                     EEADTLWRKILSGAAT"
     misc_feature    complement(18090..18104)
                     /note="TFBS match to SypG, Regulator of biofilm formation
                     and host colonization, confidence: weak, score: 19.01"
                     /tool="antismash"
     gene            18485..19291
                     /locus_tag="AW270_RS19345"
     CDS             18485..19291
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1136: GntR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012851094.1"
                     /locus_tag="AW270_RS19345"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="GntR family transcriptional regulator"
                     /protein_id="WP_082801672.1"
                     /transl_table=11
                     /translation="MAVHSRYLDLAAQIRARIEAGEWEPGAKLPRLDDFASEYGANRDT
                     VGRAIGVLESEGVVWAVQGRGIIVRHGMMRLRRPRGNLVKRNVAADGPGYSFPSASGQE
                     VWKHHVPATTSIEPLTDPRIAKLLGVPAGSSVMRRFRVTGPESEPPFQISVSWIHPRGV
                     EDVSDIDAEATSPSEWLYRLEKAGHWPITWMEIHRARMPTKDEATLLEIPTSLPVLEIV
                     RVGTSGGDGKPIEVTEYIVAGDRVETVHVLHRDESAQCPWPDEAAR"
     gene            19288..20013
                     /locus_tag="AW270_RS19350"
     CDS             19288..20013
                     /EC_number="3.1.3.16"
                     /GO_function="GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /GO_function="GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /GO_process="GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012888328.1"
                     /locus_tag="AW270_RS19350"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="PP2C family serine/threonine-protein phosphatase"
                     /protein_id="WP_061296294.1"
                     /transl_table=11
                     /translation="MSLTVTTAAGTHVGLVRRRNEDAFYCGQALFAVADGLGGHVSGDI
                     ASNTAIDALRPYDKETAPADLLTALGRMVYAANQALREKVEAEPESAGMGTTLVAMLWA
                     GSMVAFANIGDSRAYLLRDGDTDHGEAELIPLTEDHTYGNLVSDASTVPNLPPKMSRFL
                     DGRVDGRSPDLNIRELADGDRFLLCSDGLSAVVPPHLIREALRSSPDPGDAVDRLVSLA
                     TDRGGPDNITVIVVDMHKG"
     gene            complement(20079..20624)
                     /locus_tag="AW270_RS37850"
     CDS             complement(20079..20624)
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF044913.1"
                     /locus_tag="AW270_RS37850"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="WP_082801673.1"
                     /transl_table=11
                     /translation="MNATNLLKPAKLWSAAEVLVPHSPVPAAPGVYGWHFDKSPDPQLP
                     EDRLLYVGIAPRRMLTRPSRQNLRTRVRYHFRGNAEGSTLRLTLGCLLGLELRRVGSGK
                     RLTFGRAGERELSDWMAEHARVCWLLHPEPWIAETALLSSLDLPLNLDQNRNHTFHPYL
                     SGLRATAKARARALPINS"
     misc_feature    complement(20421..20423)
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
ORIGIN
        1 caaactgctg cccgtcttcg agaactattg ggaggcgtac ggcggcatcg atacccggct
       61 gcggtgggag gggctgtccg gcggtcaccc gcagcgcgcg gccttcttcg acaagaccaa
      121 gtgccccggc tgcttcacgt cgtacaagaa ctatgtcagc tacgtcctca accgcacgaa
      181 ccactacagc ggagtgaagt acaaggacga gtcgacgatc ttcgcgtggg agctgatgaa
      241 cgagcctcgc taccaggacg tcagcgcggc cgagaacacc agcgggacca cgctgcgcaa
      301 gtgggtcgac gagatggggg ccttcatcaa gggcatcgac cccaaccaca tgctcggcac
      361 cggcctcgaa ggccacgaga cgagatacgg cttcggcggt gatgagggca accccttcat
      421 ctacatccac cagtcgccct acatcgactt cacctcggcc catccctacc cgaccgagtc
      481 atgggccgac ctggacatca acgagaccaa ggcactgatc cggacctgga tctccgagtc
      541 gcacaacatc gtcggaaagc ccttctacat gggcgagttc agtacgcaca acgtcgatcg
      601 caccacatgg tggaacgcga tcttccagga gttcgaagcg gccggcggtg acggtaccgg
      661 cttctggtgg taccagaacc gcaacgtcga cgggaagttc ggcgtctccg aaggcgcgcc
      721 cgagctggcg gctttccgca gccactccgc ccgtcaggcc gccaagaacg ggccggcccc
      781 gagtggttcc ccttcgccgt ccccctcgcc atcggcgagc cccagcccgt ccccgtcccc
      841 gtccccgtcc gcgtcgcctt cgccgacccc gtcacccaca cccacctcgt cgagcgggtt
      901 cgtgaaggcg tcgaacggtt ccttcgtcct ggacggccag ccctacaagt tcggcggcac
      961 gaacaactac tacctgcact acaaatcgcc cgccatggtc gatgacgtct tcgccgacgc
     1021 ccgggacgcg aacctcaagg tcgtccgggc atgggccatg atcgaccgcg gctcactgga
     1081 tggatcggtg cccaatgtcg acggcgtggg gcacaaggac ggctactaca tgcagtactg
     1141 ggatccgtcc agcggaaagc cggtcgtgaa cgactcggcg acgggcctgc gacagctcga
     1201 ctacatgatc gcctcggcga agcggcacgg cgtcaagctg atcctcacgc tgacgaacaa
     1261 ctggaaagag ttcggcggca tggaccagta cgtgcgctgg cggggcgggc agaaccacga
     1321 ccagttctac accgactcaa ccatcagagg ctggtacaag aactgggcgt cgaccctgat
     1381 caaccgcacg aacagcgtga cgggaaccaa gtacaaggac gatccgacca tcttcgcctg
     1441 ggagctcgcc aacgagcccc gttgctccaa cggcaacctg ggatcctccg gctcctgcac
     1501 cacccagaca atcgtcaact gggcgagcga gatgtcgtcg tacatcaagg gcctggaccc
     1561 caaccacatg gtcgcggtcg gcgacgaggg tttcctcaag cggaacaacc agagcgactg
     1621 ggcctacaac gccggtgagg gtgtcgacca cgaggcgctg acctcgttgt cctcagtcga
     1681 cttcggcacc taccacctct atcccaacca ctgggggaag agcgccgact ggggcacgca
     1741 gtggatcaag gaccacaaca ccgccgccgt cggctacaac aagcccgtca tccttgagga
     1801 gttcggctac ctggaccagg gccagcgcga tggggtctac cagtcgtgga ccgacgcggt
     1861 acgcaccgga ggcggcgacg gctggaactt ctggatcctg accggcgtcc aggacgacgg
     1921 ctccctctac ccggactacg acggcttccg tgtcacctac ccctcgaaca cgtccacagt
     1981 gctgaagagc gccgccacag cgctcggcgg aagccccgac ccgagcccgt ctccttcgcc
     2041 gtccccgagc ccctctccca gcccgtcgcc gtcgccgagc ccgtctccct ctcccggggg
     2101 ggcctgcacg gtcacgtaca cgatctcgga ctggggaaac agcttcaacg ccgaggtcac
     2161 catcaagaac aacagcgcgg ctgcgatcaa cggctggacg ctgaagtggt cgttccccgg
     2221 taaccagacg atcaacagcg tgtggaatgc caaggtcacc cagagcggta aggcggtaac
     2281 ggccgtcaac gagccgtgga cggcggtcat ccaggccggc ggaaccgtca gcttcggtat
     2341 gagcgccaac tccgtcgcgg gcaccaacgg tgtcccggcg agcttcacgc tgaacaacgc
     2401 cacctgcgcg aagggctagc ccgcgagctg agggccggag ctccggccct cagctcacca
     2461 ggcgacttca cgcgatccac gttcttccgg gcatctcggc gcacggggtc gtcggcgcgc
     2521 ggttaggcac cacaccaccg gtagtcagac ggctgatgcc ggaaaaccgc cagccctctc
     2581 catggtcttc gactccaccc aaggcgacac ctcgtcgtgg aggctggctt tccgcacggc
     2641 taccaccttg acacccactc tcacactgag ggccagctcg tctatgcggc cgcaggagcc
     2701 ttggccacta cgactgaacg cggaacctgg gtcgccccgg ccaaccgcgc gacgtggacg
     2761 ccgcccggat tcgggcattc ccaccgcttc tatggcgaga ccgacgtccg tctgctcacc
     2821 atcctggccg aactatgcag cgcgctcgtg ccccgtcccg ggctgttcgc ggtgagccct
     2881 ctgctgcgag aagccctcct ggcgaagagc gggtccttcc cctgcgcagc gtggtgtaca
     2941 gccccatgag ccgcggcaag ctctacctgc tcggcgacgc ggcacacatc gtccctccga
     3001 tgagcgcgaa gggaatcaac ctcgcgctca atgacgccga ggtgttcgcc cgcgccgcga
     3061 tccagcagat ccaacagggc gattcgcgtc tactcgacag ttactccgac acctgcctca
     3121 gccgcatctg gaactaccag gcgttcgcgg catggatcac cgaaatcatg cacaacgtcg
     3181 gcgacacctc ctacgcagga gagttccgct agcagttcgc ccgcgcggaa ctacaacgcc
     3241 agttcacctc tcccacagca accagctctt cggcgaattc acctccgggg tgaactgacg
     3301 acgcgacgaa cttctgcgcc ggcgttgctc cgcggggtgc cgggcacagg aatgcgcgtt
     3361 ctccgccgat gtgcggctgg ccagccgcac cggcttcgcg ctgctccgcc accgcatcct
     3421 cttggcctaa cgctccgcca ccaccgaaag agagacagag ccgaatctgc gacagagccg
     3481 ttcagtggac agacccgccg atgaacgcac aggcgggaca catggcgaag gctgaagcgg
     3541 tccaggtcga tggagacttc gatgttgccc ggacccgcct cacctacccc tagccacctt
     3601 tgatgagggc tactgggagc agggcagggt cgctcagaca atggcgagct tctccaaacg
     3661 cgccacgcag tcgttgatca tggctggggg catgcgcttg gcggcggctt cggccgctga
     3721 ctcaggggcg gcgccgtact gccaccagac ctggccggtg gttcggtcgg tgacgaggaa
     3781 gcggtccttg acggccggtc ctcgcacgat gagggagacg gtgtcgggcc gggagacggc
     3841 ggcgtggacc atgctgtggt gcggggtgta gccggggccg ggctgttcgg tgcggaccat
     3901 gaggggctgg agggcggcga cgtcgatggt gttgtcgaag tcgtcttcgg tgccgtacag
     3961 gctgggtatg tggcggccgg acagcaggcg ggcggagtag gtccagcggt ggttgtgggg
     4021 tcggtcgtaa tggtcggggc cgaacagatg cagccggacg cggaagccgg acgggtcgtc
     4081 gtgcaggacc agcttgtcca ggatgtcgta gtgcttgcac aggctcggga ggcgctcgtc
     4141 cccgtacacc ggttcgacga cctgccgcaa ggcggtcgtg gtcctgggct ggcggagagc
     4201 aggccccagt gagcgcccta cggaacgatg cgaagcaccg gacgctggtc ggctatcccg
     4261 agaagttcac ggacccggga ggcaggccag gcgagctggc ctgccagatc gtgcatggtg
     4321 agccctgtct gctcggtagc gagttcgaag gcttgccgga ggagaactgg aagttcccct
     4381 ggatagccgg taatcgactc ggacgtgaag ccgggttggc tccggagctc atgaagtcgc
     4441 tgatagccgc ggctggcggc tgagtcggac agcaggccga cctcgcggca gcgatagagg
     4501 agcgagtcga ccgacactcc ccatttgtgg cggagctccg ccagacggcg cagatccagt
     4561 cggcctggta gctcggagag gatgctgtcc cggggggtga ggaattctgc ggcgaagctg
     4621 tccgcttcgc gttcttgccg ggagtcgcct gcgggagcgt cgccgtgcaa gatcagatgg
     4681 ccgagttcgt gagctgcggt gtagcggtag cggtgaatgt cgtcgtatcg gttggcggtg
     4741 agcacgatga tcggccgggg tagttgaagc gtggagaacg cgtccacggt cgccacgtct
     4801 ttgtccgcag gaggaagcac cacgatgatc ccgtgtgcct caaggtgtcg gacgaggtgg
     4861 gaaatcgggc cggttccaag cccccaccgc tgtcgcagag cttgagcggc acgtactggg
     4921 tcacgggaca gctctgtacc agggtggact tctccgcctg agaatccggg taggtcgaca
     4981 ggagggaacg tgacttgttt ctccagggcc aaggccagct cccagacctg ttcgaccaag
     5041 gccatggctt tggcccgctg gtacaccctg gctgatcgca ggcttcggaa gtaggtcata
     5101 gagccgtcca acttgccgtg cggccggccc gccaggaaga actgcatcgg aacaccgagc
     5161 acgtcggcca gtcgtgggac cagatcccat cgtgggcggt tcgtccctgt ttcgtattgg
     5221 ccgatggcgg ctggggagac atcgatgctg tccgcgagct ccttcttcgt catcccggca
     5281 aggtgtcgcg cctgcgtcaa cctggctggg tcgaacgcat cgaggatcgc ctgaggactc
     5341 gacgactcat cgagaccgga ctgtcccgaa atgcgtcggc cctgcttgct actgctcatg
     5401 atcctcagcg gatgcggtct cctcgtccgc ctcactgttg acggggaaga ccgcttcgtt
     5461 cttccgctcg atctcgggcc gcggggtgat cgtaaggtcg ggcagcgttc cgtggtcgaa
     5521 gcgctcggtg gattccagct ccggagtcgc aacactgcgg agacggccat cgggccgggt
     5581 cagctggatc agctctttat cgatccactt gagacgcccg aaggcgtcaa cgacgacagc
     5641 ctccccccaa tacagctgga tggggccagc cttgtcgttg gccgcgtggg cgatcagaat
     5701 cagccgcgtc ccctccggca ggtgagtcag aacgggctcg gggccggtgt cctcgggtgt
     5761 gtcccagatg gggtcctgta cgtaggaagg cttcgggccg aactggttga acaggctttc
     5821 cacaagtcca gagagccggc tctcaccaat acgcgcgtcc tcgatagggg tcgtgttgtc
     5881 cttggcgtac cggaaaggga agagcaggtt ctcgccgacc aggacgagtt cgtacggggc
     5941 ggctttaggc ttgacgatct ggacttccgg aaggtcacgg aagttctcga cgagcctttc
     6001 gtagcgaagg gtcatcagcg tcgagccgaa ggtgaatcgc ttcttgctgc ccgacacctc
     6061 ctgggcgtcc tgggcgttcg ccacggcttg gagaaacgac ttggacacca ggtctcgtat
     6121 cgccccggcc tgctcgccga aacgtctggt cgcccagttg gagctctcgt cgccgctcac
     6181 gcagacctct ccttcgcttg ggttcggtgc tcgggaacta gccagggact ttagttttca
     6241 acatcatact cccgtaatcg cttcagtcgg caactcgtcc atgggcgtcc agtagttgat
     6301 cgggacgctt gccgtcaagc gtctcgccga cgccggaagc gcataaggcg agccgggttg
     6361 tagtcgctgc ggagcccggt ccgttttaga gaccccaatg agggcttcgg gtggagaacg
     6421 tggcggctca cgtgctgcag cacttggcgc atctgctgca caacgatccc gtggtcgccg
     6481 tggcgttcgc ggtgacacct gctgtcgtcc agcggctgcg ccggatgccg tcccgaactg
     6541 gtcacggcct ggatgtcagc agcaaggtaa cggctgtaag ggcggcgagc gggacccagg
     6601 gggcggtgaa gccgccggtc aggtcggtca gcaggccgag gacggtcggt gccaggatga
     6661 cggcaacctg gttgatcgcc atcgccaggc cgagggcgaa gctggtgtgg cccggcggcg
     6721 ctgattcggc ggcgtaggcg atccacgggc cgtaccagcc gatcccaaga agccgagcca
     6781 cgtgccgttc ccgatgctgt agcagaggtg agcggctccg agctgtgtca gtagctgccc
     6841 gcgacagcgt gacggcgatg ggtgaagcgg gtgagctcgg cgatcgccag ctggagctga
     6901 acgccgacgt ccaagtttcc gtcagggacg ggatgcaagc tgatctggcc gccgctggcg
     6961 tgggcgagtt gctgaacgac cggtcaggcc caggccgatg ccgtcgtggc tgccgggagc
     7021 gtgccagaac cggtcgaagg cgaggcatgg tcggccacgc gcatgcccga gccgggcctt
     7081 ctgggcattg ggctgagggc agatcattcg ctgtcagccc tcatcgtgga ggaactgtct
     7141 gacggcggat ttaggacgca ggttgaggcc gcgatggata tcgcagacgg gcctccttgt
     7201 cacgacaatg atgtgatttg catcacactg cgagtattgc gatcggtgaa gaaggcaacg
     7261 gtttatggcg tggattgaac gtttgcggat atttgtggtt gttcctggcg atgtgtatca
     7321 atctagaccc gattttcctg ccgggtgcct gcgaagttct cgcgaagttg cgctcagttg
     7381 cggtcagtac cgcaactacc tgccccaaat agccttcagg tgtgggctac cttccgaagg
     7441 cggtgtttcg tcttcgaagt tgtgtgcgtc gaagtgagta atggaaacgc gtaggctgtc
     7501 cgtatccact ccggtgtcat cgctagtcgg tcttcatgca tggggccgac cggtttggcc
     7561 cttcggtctg tccctcggtt aaagggccaa gaaggaggaa actcagcgat ggtggctggt
     7621 aggcgccgta agcggcagct ttctggcgga gagggggctc tgcttgtggt tcgggcagtc
     7681 ctcatcttcc tggactgggt ttttaacgga gggggactca ggtagggggc tagcctcctg
     7741 agtccgttct actctccgcg ggccccgaat gatcctcgga tcgttcgggg ctttctcttg
     7801 cccgtcagct tattgctgat cagtgatttt gttgcctgta tgcgtgcgcg cctcaccgtt
     7861 atgcatgcat gcataagcgc agtctacgcc aggcttgaca cccaaataca ccgctcaatc
     7921 tcggctatac gtctatgggt tccaaagtcc acagcacgcg ctgtaggccc tgccggggcg
     7981 aggcgggtct gctggtcgtg gcgatcagat gagcgcgagc ttttccagcc gggctatgca
     8041 gtcgttgatc gtgacggggg tcatgcgctt cacggctgct tcggccgcag attcggtggc
     8101 ggcgccgtac tgccaccaga ccttgccggt cgcgcggtcg gtgacgagga agcggtcctt
     8161 gacggccgga cctcgcacga tgagagagat ggtagcgggc cgggcgacgg cggcgtggac
     8221 catgctgtgg tgcagggtgt agccggggcc gggctgttcg gtgcggacca tcaggggctg
     8281 gagggcggtg acgtcggtgg tgtcgtcgaa tccgtcttcg gtgccgtaca gggtgtgggt
     8341 gtagctgccg gacagcaggc gggcggagta ggtccagcgg tggttgtggg gccggtcgta
     8401 gtggtcggga ccgaagaggt gcagtctgac tcggaagccg gacgggtcgt cgtgcaggac
     8461 gagcttgtcc aggatgtcgt agtgctcgca caggctctgg aggtgctcgt cgccagacac
     8521 cgcctcgacg gcctgccgca gcgcggtcgt gtcctgggcc agggccgaga gcagttcccc
     8581 ggtggccgcc tcgacctggg cggcgtcgtt ccagtccagt tcgcgcaggg cccgggtgta
     8641 ggagtcgatc aacgtcatgc tggttctcct agtgtggaag agacagtggt gacggttcgg
     8701 gcgagcagtg ttcggtgccg gtccggcagg cgtggatcag gcagtccttg atgtcgctgc
     8761 cggcgagatg ggcgacggcg aatccggcgg agaacgcggc accggcgccg tgggtgtgcg
     8821 cgatcggtcc ggtgcatgcg ggctcgtggt ggaagccggt ctcggtgcgg gcgagcacgc
     8881 cgtgtcggcc gagggtcacg agcgtgatct gcgggcgtag ctgctcatag acgctgtcgg
     8941 cggctctctc ggctgcattc acctggttgt cgggaaggct ggtctggacg gcgaggaggt
     9001 tggtgccgac agcggctttc tggatggctg ggtggatcgg atcgccgccg aggttcagca
     9061 tgatcgggcc gtctgaacgg gtgacgtgct cgagggcttg gacggccgcg tcggtgatga
     9121 tttcgtagca gtcgatgtag gcgagccggg ccggggcgag cagggacatg tccgcgtcga
     9181 gaaggtcagc ggtcgccgag ctgagatggg cgaaccacgt tctggtgccg gtccgatcag
     9241 tgatgaccgt cagattcggc gtgacggtcc ccggtcgggc ggagatgcgg tgtcggaccc
     9301 cgtagcgctc cagttgcgcg aggaggtcga ggccgacggc atcttcgccg acgtcatttc
     9361 cgctgagggc cacccgcagg ccctggctgg cagcggtgat ggcggtgatg ggtccgtcgc
     9421 cggccaggga ctggagggca tcgtggacct cggcgccggt gtcggccgct gggtagcggt
     9481 cgacggtgag caggcgggcg gcggcgaggt agctgaagca ggagaggtcc acgatgctgg
     9541 tcatgtcagg accagccgcg ctcggagtac acgtcgccct tgatgcagtg ggccagcgcg
     9601 tcggcggcgt aggggatcat ctggtcgggg agcgcgtcga gggggaacca gcgccactcg
     9661 acgcatttgt cgggctccat gttggtgatc tcgccggtcc actgttcggc ctcgtagaac
     9721 agggcggtgc gggcgtcgtt ggtgtagtgg tgcatgaggt gggcgaactg gacgtcgccg
     9781 ggttcgatcg tgacgccgat ctcctcggcg gcctcgcgga gggctccctg gctggccgac
     9841 tcgccggggt cgaggtggcc gctgggcagg tgccaggacc cgtcggcgta gccggtgttc
     9901 tggcgctggc ccagcaggac ctggtcgccc cggcggagga tgaggtgcag gtccacgcag
     9961 gtcttgtagc gctcgctcga cataggtgct ccttggtggg tcagttggcc ggggtggatt
    10021 tccacagccg gcagacgggg acgatgtccg gctcggtgca ggaggcggcg ccgtcgatga
    10081 gttcctcggc gggacagccg cccatgcacg aggtggtggc cttgcagtcg ccgcaggcgg
    10141 ctttggtgag ggctccggtg aacaggtcga actcgttggg gcccttgttg atctcgatct
    10201 ggccgttcgt gctggtggcg tagttcagtt cggtgtcgaa caggaaggag cacacgtagg
    10261 cgcgtccgtc ggggaagatc gagatccggt ccagggtccg gcctatgcag ccgcgatagc
    10321 cctcttcctc gtaacggggc atctcggagc ggcgcgcgaa ggtcggctgg taccagacgc
    10381 gggtgcggtg cttggcggcg atcaggggaa gttcgtcgca gaactggagc cactcggtgg
    10441 ggttcatgcc ccaggactcg ttgtcgtggc cgcgtccgat gacggagaag acgtggaact
    10501 tcaccaggct gacaccgagg tcgtcggcga tggtcagcag gttcaggcag tcgctctggt
    10561 tggccttgtt gacggtgcag atgatccggg tgtcgaagcc gcgcccggtc agttcccgga
    10621 tggtctccag cgtggtggcg aaggtgccct cgccgcggac ggcgtcgtgg gtggcgggtg
    10681 atccgccgtc catgctgatc tgcacgtagg cgaagtcgga gggctccagg cgccggaact
    10741 tcctggcggc cgggcccagg ccgttgctgg tggtgatgac gtgctcgtac ccgagctcgc
    10801 ccgccagccg gatggtgtcc tcgtagcgcg ggtgcagggt cggttcgccg ccgaggatgg
    10861 tcaacttgga gccgcccatc tgccgccagg ccgtgaggtt gttacggatc tcctcgaagg
    10921 gcatcttgag ggcgcgttcc agccgctggc ccatgtagca ggagccgcag cggagctggc
    10981 acgcctcggt gatgtagagg tagacgttgc ggaacctgcc atagggcacg cggtcgatgc
    11041 gccggacctc ggggtcgtcg gcagagactg gcatcctggc gtggccgtag gcatcgctgc
    11101 cggtcctgat cgccggcatc gggaggtcga cggaagtcac tggctactcc tggtcgaggg
    11161 acagttggag actgcgtcca gagcgacggc gaggacctcg caggccggag ggtggctgtc
    11221 gaccgtgcgc cacggaatcc cgtgaccggt cagagccgtg tagatctcgg gcaggcggct
    11281 ggtgaagcgc tgggtgcggg agctgagccg gtccgggttg tcgtcttggc gctgaagcag
    11341 ggtgttgcca catctggcgc aggtatcggc ggtggtgacc tgggctggct tgtgtggttg
    11401 tcccgtggcg gttggctcgc agtggaggca gacgcgccgg agttgagctc gttgcagagc
    11461 ggttgcccag tcggcgcgga ggtggatcac cgtcgcggag acgtccaaac gggtcaacag
    11521 gtccgccagg tgccgggatt gcacaccgtt gccggggtaa ccctcgaaca ccacaggtgt
    11581 ttcaccaggc tggaagggac cggagatggc gtcgtgaacg agctgggtgg cgatgtcgtc
    11641 cgggagccag ccgagggagt cccggctcgt ccctttgaga acagccgtct cagcttcggg
    11701 aaggctctgg acgtactcgc gtaatcggaa caaccgtgcg ttgcgcttgg ctacgagttg
    11761 ctcggacagg gtgctctttc cgctcgctgg cggaccgaag aggatcacca ccggccccgt
    11821 cacgacaccc tcccgttagc cctgaagtag cggatctgtc ctgccggggc caggccgacc
    11881 gcgtgcaggt cggacagaag gtcggtagcg ctgtccggaa cgatcgcgcg caccaccggg
    11941 gcgtgatggc ggagcccggc ggcgagggca gcggcgatca gcggcgcggc aacgtcggac
    12001 cgctcggtgg ctgggtcgat gaggacatca gccagcaatg cctcagtggg ttcggcaggg
    12061 tccatatggg ccaacgggta catcagggcg gctccgcgga gttgatcccc ggttccggcg
    12121 gcgatgagaa ggccgtcgct gacgaagcgt tcgtctgcgg tccagcgcag gtaggtgtcc
    12181 tcggcgacct gctgcccatg ctcagggtga atggactggt aaaggcggct cagggcgtcg
    12241 gcatcggaag cttcggcctg ccgtactccc acacggccgg ggtgaggaac atccggcagg
    12301 gcagtgaggt cggctttcag caaggtggcg gtggaatgga gctgccatcc agcccgctcg
    12361 aagaattcgc acccgtgttt gcgagcctcc gggatttcgg cggtgagcag gcttgctcca
    12421 ccgtcgtggc atctaaccgc ctgctgcagc aacgccgtac cgagtccgcg ccgctgccat
    12481 tctggagcga cgatgggacc ccacagccgc gccgacaagc cgggtgccgt cccatgccga
    12541 agcgcagcag cgccggccag tgtgccgttc ggcagacggg ccaggagcat cgtcgtcggc
    12601 tgggcattgc cgttgcggcg gagccgggag cgtactagag cagaggtcac cggccgttgg
    12661 tcgggccagc cgcgcatggc ggcgtacccc aggtccgcaa gcttctcgtc cagcgcggtg
    12721 ccgggcagat agacaacgct actgattggc ggtgtcatgg gcgcctcggt cgggtggctg
    12781 ctatgccgac gcagacgctg ccgcgtccgg tcagaaaggc gggatggaga gccagcgcgg
    12841 ccgtgggagg ggcgctacgg gtgaacacgc gaacctggcc gaacctgctg cgaaagacgc
    12901 tggcgatttg gttttgagag gcgaacgggc tgggccggga cggacggcga agccgctgtc
    12961 gggcgagatc catccgctct gccgaggtcg cgggccacgt agtggccgtc agggcctgtc
    13021 caactcggcc gatccggtcg tactcaaggt ggccggcaag cccggcgacg cgggtggtgt
    13081 agagcagcag gccgtcgtcg gaaagaagac ccgccagccg ggcgagcacc gcttggcggt
    13141 cggccgggcc gggcagcagt gacagcagtc catcggtgac aaccaggtcg aacggcccag
    13201 ctatcccgca gagctcaggt gcttcggatt gcagggcggc aacctgcaca ccggtttcct
    13261 tcgcccaagc gttgatcaga accaacggtg tgggacagga gtcggccacc gtgatccgca
    13321 tgctgggctg aagctcggtg aggacctgcg gcatggtctc gtccgaggag ccgcacacca
    13381 gggcttccga cggctgcctg ccggccagcg cacgctgata gaaagcgctg tgccagcggg
    13441 ggttgccctt cagacccgtt cgccggaaca gccgccacga ggcgtggtag cagaaacagc
    13501 cccgggcgca gttctcgcgg gcggcggtcc aggcgcctct gatcgtgaga gagcccgtca
    13561 ttgtttccgc cctatgaggt ggaatagacg ggcgacctgt cggtaggggt cacgacatcc
    13621 ggcctgccac tcggcttcca ggacgtagtc gaatccttcg ccgaccgggg cgtcgccgag
    13681 gtggtcggtg aagacccgca ccccgtacca ctcggaggtc gtcacaccgg cgtccccgag
    13741 gatcgtctcg actgcggcga gagtgtcggc cctgctgctc accccgaggt tgccctcaac
    13801 ggaaccggcc cccatcaccg cggccgcatc cgcccaccgg ccttccaatc cggggcgcat
    13861 cgccaatgcg tccgcgttct tggtcagcag cgatagcagg ccgcccggcc gcaccgccga
    13921 cgcgaggtcg gtcagaaagg tgtgggggtc ctcggcgtac atcagcacgc cgtggcaaca
    13981 gacgaggtcg aagccgttct cggccaggtc tctggcctga tcggcggtgc cttcgaccag
    14041 catgacccga gtacggacct gatcgctttc cgccgtcagc cggtcggtag cccgcttgtg
    14101 catgccagga tcggggtcta gcagtgtcac tcgatgtccg gcccgtgcga gcaggacggc
    14161 ctggtggcca tcgccgccgc cgacgtccag gacctgctga gggtcggcgg gcagatggat
    14221 gcgcagggcg cgggcgacca gcgcgtggcg gagtgcgcct cgcaacgtcc cggcgtgacg
    14281 ggcgtaaccg gccgacaggt cggctccgga cgccgatgtc cagctgttgc tcacgggtgt
    14341 gtctccttcg gcgagagcag cgaatcgaac aggtcctggt gctgaagcac caggcggatc
    14401 gacaggtagc gggtccgcag cagctcgcgg agcacgcgga cgcccggcgt gtcggggagg
    14461 gtgccctcgc gcgcggcgcg gcgcagcgcg atcagctggg gtatgcgcgc ttcgacctgc
    14521 tgcaggtcga tcgggaggtc gcggtccagc cacgagcgga gcctctcgac ggtcatgacc
    14581 tcccacacca cggcatcgcc ccagactccg tccgggcctg cggcggtgac caggtgcggg
    14641 atgcggcaca gccgtgattt gggcagtggc ggcggtaccg ggctggtcac acatgcggcc
    14701 tccccactgc gacggccccg gatctggctc aggtgcctgg agaaggccag cgccagaccg
    14761 gtctgtgcca aagcgattcc ggtcggggcg atggccacgg cttcgaccca cgagcgctgg
    14821 gccgaggtca gcgcgaggcg tacggcggcg aggggatcgt gttcctcgtg ccagacccgc
    14881 cgtcgtgccc cgcaggtgac gacccccgga ctgagcagga ggccggcgtc ttgggcggta
    14941 aagagccgct caccctgggc ggtgatcacc gggtcgcctc cgggccgaac cgcgcgtagt
    15001 agcgctcaag caacccgcag gactccagga cccggttgga ggcttccacg ttgtgccccg
    15061 cgatgaagcc gtgatctggc gccgcgaggg tggacgcggc caccaggagg ctggcgagga
    15121 tcaggtcgga gtacatgttc ggcagggact cggcgtggtg gaaattgatg gggaagccgt
    15181 cgcccacgag cgtcacctgt acacccgatg gaagatgcag ccgcgttccc agcacacctt
    15241 cgtcggtgac cagccgcgcc tcttcttcca gctccgcgac cgcgaactcc cggttacgag
    15301 aggtcgtgga gaccaggtag cagttccggc cgatcgcggc ggcatgttcg ccgcgtaacc
    15361 cggtccggcc ggtgcagccg atgaccagca gcggccggtg gtcgcgcagg agggtgctga
    15421 ggcaccggtt ggtgaggtat ccgcgttcgt gcgctgcgat caggcgaacc agctgccggt
    15481 catggacggc cacgcgcatg cggcgtgatc tcaggacgtc ggcgatgtgt tcgccgatcc
    15541 gcccgaaccc gaccacgagg gcaggccggc cttcgagttt ctcctccggg agaaggcgca
    15601 acaggttgcg gaccgcggcg tcggcgatgc cgtagcactc taccgtcgcc ttcagccgcg
    15661 actcggcgac cgagaagatc ggcagtggaa gccgcccttc ccagggttcc agccgggtga
    15721 tgcccgagat cgtctgctcg acgaggccga tgaagtgcgg aagcaagtcg gggcgctgcg
    15781 ccagcaggac tggaagtgtg tagccaccgt cgtcgatcag cagtcctcgc aggccgaggg
    15841 cggcggcacg ccgggtgtga tcgtccaagg cgtccgtcgc gttcgtccac ggccataccg
    15901 cgatgccttg cctggtcaag tgggcgtcta cccggtcggt gtgctggtag gggtacccct
    15961 tgtccaacac agtgatcgcc tgcgcgggca cccccatcgc caggaccgat tccaccatca
    16021 ccaggaaatc ggtcatgtga tgaaccgtca gcaccggagc cagcgaggac agagttcccg
    16081 gcggaagaaa ggcgggcagt tcgcccgtca acggcatctg accgcgcagc gccttcatct
    16141 cggtcgtggt gaacggaatc cgtcgtacgg cggtctgaag cgtctgcacc tggtcgggag
    16201 acaggccttc cgtctcctcc atgatcaggg tcgcgatgtg ggtgtccgag gaggtcgcct
    16261 tgggtcggga cggcgtgctg acaaataggc ggacgcatgc ctcggttccc agacgtaggc
    16321 gtgtgccggc gccgtcgtgg gttgcctctc cgatgtcagt gaccttggcg attgcctcga
    16381 tcaccgcatt acgcaccatc aacaaatctg cagcttcacc acggatctcg gtggttacga
    16441 cgctctccat ggatatcagg aacgcccttc cgcggtcggc cgcgtcgggg cggtgcgcct
    16501 gtcacctgca ctgctcgaaa agttgatcac tcgtgtgctc ccttgctgac gtgaccgaga
    16561 ctcggccgaa ccgatgaaga cggcaggagt tcgtacaggt aaggggaggt ggcggcgtcg
    16621 catggcgata ggtccgtgag acgccgccgg agggcgaggg caatgggcaa ccctcgctcc
    16681 tcatgtacgg ccgaactgtc ccctccttca ccagcagggc cgtacgtcgg atggcgcgat
    16741 acgccgcacc cgcatcgcgc cccatggtca gcggggcggc aggacgccgg tgcggatgag
    16801 gctcatttcc gcattgagaa cggctcggcg aagttccttc ggcgtgggcg cggtgacgac
    16861 gagcggcgtg tcgaccggcc attgggccat ggcgcagaac tcgcgctgcc aagtcagcca
    16921 gatgaccagc cagccggagt gctcacggtt gagttcgtcg gcgaagcgat gcccggcgta
    16981 gtggtcgatg cgccggtccc ggcgggcggt acgggatgag cccgggtggt ggtcgccctt
    17041 ggggtgcttg aaacggtcga tagctcgacg gaggaccatc acgactggta ctcctcgtcg
    17101 gctcgctggg cgatctgttc ctggctgttg atcgctcggg ccaaagcggc gaggtcgtcc
    17161 gcgtcgacgg ccggagtggc gcccgcctcg cgtgcggtct tgccgaagtc ctccttccgc
    17221 caggcccaca agcgcccgac gtcgctggtc aggtatctcc acgtgtcggg aacctcgggc
    17281 gtcgggctgg gcatggcgcg gacctccggt gcgtgggagg aagaagtaga gggacgaggt
    17341 gccttcgacg tgagcggacg gctcggaggg cggttcggca tcggccgggt gcgggaaacg
    17401 accgtgcttc ggggacggtc ggtacgacgc cgctgcgccg gcaccaacgc gggagtctcc
    17461 tccgaggggg tgacagctgc gggagcggtc ggccggggaa gagtctgagg tccggtcgcg
    17521 cggcgggccg cgagacgtcg ggccgcctgg tctgaactcg gggggacgta atcgtggtcg
    17581 tgctggtcag agcatgcatg cgcgtacggg gcatggccgc agtcgccaca ccccatgggc
    17641 atgggcatcg attgaccgaa gcagacgcag ttctctgagt gcgcgatgcc tcgttcgagg
    17701 gtcgcactag tgttggccac gggtcagggc tccatcctga tcaagggctc gtccggtcgt
    17761 tgcagcgccg ggcgggcccg catttgtgcc tacggctgtc cagattcgtg cggaagtgac
    17821 tacttagagt gactgtcgaa gacgtgcgtc aacgctgtcg actgccaaag ggcttgggga
    17881 gttatgtagc cgcgccggag aggattttgc gccacagagt gtcggcttct tcgtgccggg
    17941 tcggcgggct ttcaagcggt acggcgccgg agccgtcgct ctggttcgtg tgccggatca
    18001 ggtagctgcc gccgaccatg agcttttcgt agacgatcgg ccagcaggcg caggtccagg
    18061 cgatcacatg cggtctgcgg gcgaaccgtt tcgctgattg agtcggccgc caatgcacgt
    18121 gtttgggctc ggcgcggaga accggcaggg gtaagtggtg gtcgcacgtg gactgttgcc
    18181 cattcatggc ctttcctctt gttcggctgg acctgccgtc gctcgtcaat ggccgggcgg
    18241 gggtgggaag actagggttc gacgggtcag aaatgcctgc ctggtcgagg gttgtcgcgg
    18301 cgctggctac gccgagcaag cctcctgtct gcgggggtgt gtctcatgag ccgactgtcg
    18361 tcagtgcgag ccctggtggc tactccagcg tttcatgagc agattgtctt gggactatag
    18421 tcagacctcg ctgccctgct gtcaattatc ccaagacaat ttcagtggaa gatagggttg
    18481 tgtgatggcc gtgcactcgc gttacctgga cctcgccgct cagatccgag cgcgaatcga
    18541 ggccggtgag tgggaaccgg gagccaagct ccctcgcctg gacgacttcg catccgagta
    18601 cggcgccaac agggacaccg tcggccgggc gatcggtgtg ctggagtctg aaggggtcgt
    18661 ctgggccgtt cagggccggg gaatcatcgt ccgacacggc atgatgcggc tgcgcagacc
    18721 acgagggaac ctcgtgaagc gaaacgtcgc cgccgacggt cccggctact ccttcccctc
    18781 ggcatccggc caggaggtct ggaagcacca tgttcccgcc actacgtcga tcgagccgct
    18841 gacggatccg aggatcgcga aactcctggg agtgccagcg ggcagctcgg tgatgcgccg
    18901 gttccgcgtg accgggccgg agtccgaacc accgttccag atcagcgtct cgtggatcca
    18961 tccgcgtggg gtcgaggacg tctcggacat cgatgctgag gcgaccagcc caagcgagtg
    19021 gctgtaccgg ctggagaaag ccggtcactg gcccatcacc tggatggaga tccacagagc
    19081 ccgcatgccc accaaggacg aggcgaccct gctggagatc cccaccagcc tgccggtatt
    19141 ggagatcgtc cgggtgggga cctcgggagg tgacggcaag cccatcgagg tgacggagta
    19201 catcgtggcc ggtgaccgcg tggagaccgt acatgttctc caccgcgacg agtcggccca
    19261 gtgtccgtgg ccggatgagg ccgcacgatg agcctcactg tcacaaccgc tgccggcacc
    19321 catgtcggcc tggtacgccg ccgcaacgaa gacgccttct actgcggtca agccctgttc
    19381 gccgtcgccg acgggctcgg tggccatgta tcgggtgaca tcgcgagcaa cacggccatc
    19441 gacgctctgc gaccgtacga caaggagacg gcgcctgcgg atctgctcac cgctctgggg
    19501 cggatggtct acgcagcgaa ccaggcgctg cgagagaagg tcgaagccga gcccgagtcc
    19561 gcagggatgg gcaccacact ggtggcgatg ttgtgggccg gctcgatggt ggcattcgcg
    19621 aacatcggag actcccgcgc ctacctgctc cgcgatggcg acaccgacca tggcgaggct
    19681 gagctgatcc cgctcaccga ggatcacact tacggcaacc tggtctccga cgcctccacc
    19741 gtgccgaacc tgccgccgaa gatgtcccgt ttcctggacg gacgggttga cggtcgttcc
    19801 cctgacctaa atatccgcga gctggcggat ggcgaccgct tcctcttgtg ttcagatggg
    19861 ctgagtgcgg tggttcctcc gcatctcata cgcgaagctc tgcgttcatc acctgatcca
    19921 ggcgacgcag tggatcggct tgtgtcactg gcgaccgacc gtggtggtcc cgacaacatc
    19981 acagtgatcg ttgtcgacat gcacaaaggc tgacgcggga cgctttcgcc gtcgtggccg
    20041 aagccggtgt actggaagct caggcacagg ttagagggtc acgagttgat ggggagagcg
    20101 cgcgcacgag ctttggcggt ggcacggagt ccgctcaggt aagggtggaa ggtgtggttc
    20161 cggttctggt cgagattgag cgggagatcc agactggaaa gaagagcagt ttcggcgatc
    20221 cagggttctg gatgcagaag ccagcacacg cgggcatgtt ctgccatcca atcgctgagc
    20281 tctcgctcac cagctcggcc aaaagtcaga cgcttgccgc tgccgacgcg gcgtagttcg
    20341 aggccgagca gacaaccaag agtgagccgc agcgtggagc cttcggcgtt cccgcggaag
    20401 tgataacgaa ccctggtgcg taagttctgc ctgctggggc gagttagcat ccggcgggga
    20461 gcgatcccga cgtagagaag acgatcttca ggcagttgcg gatcgggtga cttatcgaag
    20521 tgccagccgt agacgccggg cgcagcgggc acgggactgt ggggtaccag gacctccgct
    20581 gcactccaga gcttggcggg cttcaggagg ttagtagcgt tcatcgtgtc tcccacaggt
    20641 cgaatagaag cctgagcatg ccgcctcgcc aagggcagcg ctcgatccag ttcgggagag
    20701 ggtaatcctg gtcccttggc ggacctcaac ttctaccccg tcggctctaa gcaactggct
    20761 gctccgagga cgacgagcaa gagcttaaga gacatgcgcg cccgtgggtg gtgtccaaac
    20821 ggccggaagt gattctgttg cgcgttggta ggcgagcctg agggtggcgt gtcggatggc
    20881 tgcggttgcg ccggtctgga ccatgagcac ttcgcgtagg ggatggcgcg attcgatgag
    20941 gtcgttgttg cgctgttcga gccagggcat ccacgtctcg tgaggcgctc gatcccattt
    21001 cgcccggtta cagggctcgc atgacagaac caggttccag atggcgtcga cgtcagggcc
    21061 cttccagctg cgggtgagca gcttccaggg cagcacatgc tcgacgatgg gcccaggcgt
    21121 cggcatgggc tgattgcagt
//
