LOCUS       NZ_BBXG01000006        20906 bp    DNA     linear   CON 08-AUG-2024
DEFINITION  Herbidospora cretacea strain NBRC 15474, whole genome shotgun
            sequence.
ACCESSION   NZ_BBXG01000006
VERSION     NZ_BBXG01000006.1
KEYWORDS    WGS; HIGH_QUALITY_DRAFT; RefSeq.
SOURCE      Herbidospora cretacea
  ORGANISM  Herbidospora cretacea
            Bacteria; Actinomycetota; Actinomycetes; Streptosporangiales;
            Streptosporangiaceae; Herbidospora.
REFERENCE   1
  AUTHORS   Komaki,H., Ichikawa,N., Oguchi,A., Hamada,M., Tamura,T. and
            Fujita,N.
  TITLE     Genome-based analysis of non-ribosomal peptide synthetase and type-I
            polyketide synthase gene clusters in all type strains of the genus
            Herbidospora
  JOURNAL   BMC Res Notes 8, 548 (2015)
   PUBMED   26452464
  REMARK    DOI:10.1186/s13104-015-1526-9 Publication Status: Online-Only
REFERENCE   2  (bases 1 to 375251)
  AUTHORS   Hosoyama,A., Oguchi,A., Ichikawa,N., Kimura,A., Ohshita,R. and
            Fujita,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAY-2015) Contact:Director-General Department Of
            Biotechnology National Institute of Technology and Evaluation,
            Biological Resource Center (NBRC); 2-49-10 Nishihara, Shibuya-ku,
            Tokyo 151-0066, Japan URL :http://www.nite.go.jp/nbrc/index.html
COMMENT     ##Genome-Assembly-Data-START##
            Finishing Goal                    :: Noncontiguous Finished
            Current Finishing Status          :: High-Quality Draft
            Assembly Method                   :: newbler v. 2.6
            Genome Coverage                   :: 71x
            Sequencing Technology             :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_001570605.1-RS_2024_08_08
            Annotation Date                   :: 08/08/2024 02:39:29
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,566
            CDSs (total)                      :: 7,487
            Genes (coding)                    :: 7,430
            CDSs (with protein)               :: 7,430
            Genes (RNA)                       :: 79
            rRNAs                             :: 4, 5 (5S, 16S)
            partial rRNAs                     :: 4, 5 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 10 of 57
            Pseudo Genes (incomplete)         :: 49 of 57
            Pseudo Genes (internal stop)      :: 3 of 57
            Pseudo Genes (multiple problems)  :: 4 of 57
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 12:10:14
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 347558
            Orig. end                         :: 368464
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            BBXG01000006.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..20906
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="terpene"
                     /region_number="2"
                     /rules="(PT_phytoene_like or phytoene_synt or Lycopene_cycl
                     or Lycopene_cycl_fung or T1TS or T1TS_KS or T2TS or TS_UbiA
                     or TS_Pyr4)"
                     /tool="antismash"
     cand_cluster    1..20906
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(PT_phytoene_like or phytoene_synt or
                     Lycopene_cycl or Lycopene_cycl_fung or T1TS or T1TS_KS or
                     T2TS or TS_UbiA or TS_Pyr4)"
                     /kind="single"
                     /product="terpene"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..20906
                     /aStool="rule-based-clusters"
                     /category="terpene"
                     /contig_edge="True"
                     /core_location="[10000:10906](-)"
                     /cutoff="20000"
                     /detection_rule="(PT_phytoene_like or phytoene_synt or
                     Lycopene_cycl or Lycopene_cycl_fung or T1TS or T1TS_KS or
                     T2TS or TS_UbiA or TS_Pyr4)"
                     /neighbourhood="10000"
                     /product="terpene"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(10001..10906)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(PT_phytoene_like or phytoene_synt or
                     Lycopene_cycl or Lycopene_cycl_fung or T1TS or T1TS_KS or
                     T2TS or TS_UbiA or TS_Pyr4)"
                     /neighbourhood="10000"
                     /product="terpene"
                     /protocluster_number="1"
     misc_feature    complement(1366..1382)
                     /note="TFBS match to GnfM, Regulator of nitrogen-fixation
                     genes, confidence: weak, score: 19.16"
                     /tool="antismash"
     misc_feature    1374..1394
                     /note="TFBS match to NrtR, NAD synthesis repressor,
                     confidence: medium, score: 27.36"
                     /tool="antismash"
     gene            complement(1405..2847)
                     /locus_tag="AW270_RS14385"
     CDS             complement(1405..2847)
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1041:
                     transcriptional regulator, SARP family"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012889319.1"
                     /locus_tag="AW270_RS14385"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="BTAD domain-containing putative transcriptional
                     regulator"
                     /protein_id="WP_061294796.1"
                     /transl_table=11
                     /translation="MVILRALGPFQAVSDGRPLDLGGQRQQAVLARLLVAGGRAIPVST
                     LIDELWPGRPPVQALSTIQGYVSRLRRALEPGRAPREEAGVLVSAPPGYALRAEIADVD
                     AWHFEHLVKRDDGGDLEATLARLDTALALWRGPALAEFGDLPWAQAEAQRLDDLRLIAV
                     ERRADAAVRLGRSAALIPDLEAHASAHPLREEAWRLLALALYRAGRQADALGALRRARD
                     VLRDDLGLDLGPALQRLEQDILGQAAHLDGPVAAAPATTGTRPADVLRVVVADDQALVR
                     TGLKMVLDSEPGLEMVAEAENGEQAIQQVQLVRPDVVLMDIQMPRLDGLAAARRILAGT
                     DPPKVIMMTTFGTDDNLYSALRAGVSGFVLKTSAPEQLIAAIRAAVAGDALIDPAVTTH
                     LIAAFAGRHNPAAPGGLSDSEVDLVRLIARGFTNRQIAVTLAVEEAEVAAEVAGLLKRL
                     DLIDRAQLVVLAYESGLVSPGA"
     gene            complement(2872..4866)
                     /locus_tag="AW270_RS14390"
     CDS             complement(2872..4866)
                     /GO_function="GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA]"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1048: sensor
                     histidine kinase"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /locus_tag="AW270_RS14390"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="sensor histidine kinase"
                     /protein_id="WP_063819201.1"
                     /transl_table=11
                     /translation="MNDRGPGQALVIAAVVGVVSVAAVGAAVAIPYRLPAHLQEAFRPT
                     PDFTAGVTFPALGAYIAARRPRLPIGWLMIAGGLCEAASVLFSALMVQAEHGGDLAAAG
                     VLRVVGVAAWSLGGGILATLVPLFAIGGRRPSRGWSLFGVLAVVVIAVQTAVRVGRPTP
                     DAPVAGMSAVIPNPLEFVPGAIYRPLADTVWTVEQGCILLALVSMVVRMRGADPETRRA
                     IAWPALTFAVYVVLVVTGDPAFFTFLTFWTALIPVAITFSVLRHGLFGIDDVLSRTVVV
                     AGTIAGVSAVYFGTGAVFSLLMSEYDIVAGLAAALFAGVFYQPLRRLLQRGMDRVLYGP
                     VGDPRTLTARLVEEVRNADPSEALAAVVDVVRDGLAVGGARVEVVNGLRVSSGDVGDAP
                     KEHPLVWHGEPVGRLLLGAPGTRRFGAAHDERVIATLLPYVADVAHAVRMAGDLQRSRE
                     RILAAREEERRRLRRDLHDGLGQTLGAMAMTLNMARLTMKDSPGTADHLLHDLRTGMDA
                     VAGDIRELVYGLRPPALDDLGLAEAIRTLAAETLEPGTATHVSADGPLDDLPAAIEVAA
                     YRIAQEALTNVRRHAHARRVSISLGLDADLLTLKIADDGIGLPEVRRAGVGTSSMRERA
                     AELGGSCAITAPDTGGTVVTARFPLTGTM"
     misc_feature    4879..4899
                     /note="TFBS match to NrtR, NAD synthesis repressor,
                     confidence: weak, score: 19.27"
                     /tool="antismash"
     gene            complement(4894..6108)
                     /locus_tag="AW270_RS14395"
     CDS             complement(4894..6108)
                     /GO_function="GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1202: major
                     facilitator transporter"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020541533.1"
                     /locus_tag="AW270_RS14395"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="MFS transporter"
                     /protein_id="WP_061294798.1"
                     /transl_table=11
                     /translation="MTAPPVERSPVRGFIHSRVGGLPREFWVLFSGTAVNKLGTMAQPF
                     MGIYLTQSRGVSVAVTGLVLTLWGCGSLLSQPVAGFLADRVGRRATLAGGMLATAATML
                     FLGYAHDLTAIAAGMFVLGLVVDIYRPASQALLADVVSPQDRPRAYGLLFWAINLGFSV
                     AMVAGGRLASGGFLLLFWIDAASCLVFAYLVWRLIPETRPPASERLPGGYRDVLRDRTM
                     VAFVLMNLAYTCTYLQAFQTLAIAMTQRGLPPEAFGAAIAVNGALIIVVQPITTAWLTR
                     FSPSLVLAAGFAVVGAGFGLQAFATTTLQYALAVVVWTCGEILTAGMPLAITSALAPPH
                     LRGRYNGLYGLSWSVGAAVTPILGTRLLEVGHEALWLTTAGLGLVAAAGQLLLAPAIRH
                     RTNTQ"
     misc_feature    complement(6149..6169)
                     /note="TFBS match to MntR, Manganese transport regulator,
                     confidence: weak, score: 21.0"
                     /tool="antismash"
     gene            6200..7165
                     /locus_tag="AW270_RS14400"
     CDS             6200..7165
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020541534.1"
                     /locus_tag="AW270_RS14400"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="DUF5937 family protein"
                     /protein_id="WP_061294799.1"
                     /transl_table=11
                     /translation="MAVEWSFTPGDVARLRFAFSPMWEVVASLRAVQGAALHPAQLPWL
                     REVRDRLATLGLEELTGLVPAAGYLPDFLTPTPDTHQPDFADELALVRAADPEVARSEV
                     ARSAPRRAGVAARFAADPAGALAWTGDALEAYWNLVFAEFWPKVRGVLEADVLYRSRRL
                     TEGGVSDLFAALHPSVTWHGDRLITHKDCIHLAELGGAGLVLLPSVFQRLDRVAIVHEP
                     YRPMLIYPARGTGNLWARGPEEVPGALSALIGRTRARILTALETPASTSGLARVMELTP
                     GAVSQHLTVLSDGGLVSRQRLGREVFYQRTPIGDALVGRP"
     gene            complement(7275..9128)
                     /locus_tag="AW270_RS14405"
     CDS             complement(7275..9128)
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA]"
                     /GO_function="GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /GO_process="GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1048: sensor
                     histidine kinase"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: protein motif:HMM:NF019350.4"
                     /locus_tag="AW270_RS14405"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="histidine kinase"
                     /protein_id="WP_157521945.1"
                     /transl_table=11
                     /translation="MTGASPEAETRPRWMRVPDRAARVLAAGIAGAAVVLVVADLVIGA
                     PLPFTPEDVMGLAFPVFGALLVQRRPKLVIGWLLALGGLGAAASVAAADLAALLVREGG
                     HPLAAPLDVFSHGVWNLTTYALGVLLPMLYPTGALLSRAWCWPGGFAAAVLAAEWLAAI
                     AGPGPSWAGRTLQGLVTLSLVIAFSSLVLRFRRAGEDERRQVLWPLLAISCLVVPWIIG
                     SSVWWVTTITIPLIPAAITLAVLRYRLFGIDTLITRALVGAGMVTVVASVYVLAGTASS
                     LFLAEVDRYFGLVAALGAGVFFHPMRRALHRLADRALYGTRGDPVTLADDLRGRLQQAD
                     PVHGLLATLETIKEGLAVTGTALDLDGTRTSWGELPVVARRVELVWHGEPVGTLLIGPP
                     GPRRFPAAHDERVIAVLTPYIADAAHTMRLTTALRRSRERILTAREEERRRLRRDLHDG
                     LGQTLSAMAMRISMARTQLRGSPEIADTLLADLRGGMDAVSGDIRELVHGLRPPALDDL
                     GLAGAIRQLGPIDLVVEGDLTGLPAAVEVAAYRIAQDALRHTAATAITVSRGADLVVEI
                     RAGGRPEAGLSSMRERAAELGGGCEIGTSGEGTTITARIPL"
     gene            complement(9196..9957)
                     /locus_tag="AW270_RS14410"
     CDS             complement(9196..9957)
                     /GO_function="GO:0030248 - cellulose binding [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF041144.1"
                     /locus_tag="AW270_RS14410"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="expansin EXLX1 family cellulose-binding protein"
                     /protein_id="WP_157521947.1"
                     /transl_table=11
                     /translation="MRHARRRRRRGVVWAVAALVAAAVLVQLTGPVCASPERRGRAYFY
                     EAKAPGNCSLPGHPMLFASVSTPEYDGSAACGGYLDVTGPAGTVRVLVVDRCPACGPGD
                     LDLSAEAFVSIADRDDGSAAITYRRVRDPEPGRRLAFRVKPGSTAHWSAVQVLDHGNRL
                     ARVRIKNGVHWRDLRRGADNYWVFPPEPGPGPYTIRVSDVYGGQATVTGIRLRPGATQR
                     SGERLYGVVPPVVRTITTPSDRPSPESPDIC"
     gene            complement(10001..10906)
                     /locus_tag="AW270_RS14415"
     CDS             complement(10001..10906)
                     /GO_function="GO:0010333 - terpene synthase activity
                     [Evidence IEA]"
                     /GO_function="GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /GO_process="GO:0046246 - terpene biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     terpene: T1TS"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1052: Terpene synthase/cyclase metal-binding domain
                     protein"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF040195.3"
                     /locus_tag="AW270_RS14415"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="terpene synthase family protein"
                     /protein_id="WP_157521949.1"
                     /sec_met_domain="T1TS (E-value: 7.1e-40, bitscore: 132.0,
                     seeds: 237, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTPELRIPSAAAVLAPLVYCLPPTVGEPVVCDTRALGAWAGLYGL
                     SIAARPERLAARVLPGNELLARWVTWQLAYDDWCDEGSGSRDTTVLDLMQGRLREAAHA
                     GHRTEEPLVAAFADLWRVTAPDMSPAWEQRFTQRIEAQAQACRMQAVNRSIGRVPSAGE
                     YPALRRDVFAGAGAEIIEGCLRIEVPGRLHATPPWRTLLDAVVDVTAWCNDVASLGKER
                     GDPHNYVRVAVSRIGVPAPAEWVADRIVERAEDLTDAAKALPESYAELGVPDDVEKVAA
                     ALFGVPRAYLEWLLESPRYQ"
     misc_feature    complement(10977..10994)
                     /note="TFBS match to ArcA, Repressor of carbon oxidation,
                     confidence: weak, score: 14.1"
                     /tool="antismash"
     misc_feature    complement(11012..11027)
                     /note="TFBS match to DasR, N-acetylglucosamine dependent
                     repressor, confidence: weak, score: 19.73"
                     /tool="antismash"
     gene            11049..12410
                     /locus_tag="AW270_RS14420"
     CDS             11049..12410
                     /EC_number="6.3.1.-"
                     /GO_function="GO:0004356 - glutamine synthetase activity
                     [Evidence IEA]"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_process="GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013151975.1"
                     /locus_tag="AW270_RS14420"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="glutamine synthetase family protein"
                     /protein_id="WP_061294803.1"
                     /transl_table=11
                     /translation="MDRQQEFVLRTLEERDIRFIRLWFTDVLGFLKSVAIAPAELEGAF
                     AEGIGFDGSAIEGFSRVYESDMLAKPDPSTFQILPWRSETPGAARMFCDILMPDGSPSH
                     ADPRWVLKRTLARAADMGFTFYTHPEVEFFLLRDRPERGVRPEPIDDGGYFDHTPHSAG
                     HDFRRNAIMMLEAMGISVEFSHHEGAPGQQEIDLRYADALSTADNIMTFRLVMKEVALE
                     QGVWASFMPKPFTEYPGSGMHTHMSLFEGDRNAFYEPGSDYQLSKVGRSFIGGLLKHAA
                     EITAVCNQWVNSYKRLWGDTEAIAGAGGEAPSYICWGHNNRSALVRVPMYKPHKGGSTR
                     IEFRSLDSACNPYLAYAMILAAGLKGIEEGYDIPAGAEDDVWALTSGERRALGIHPLPQ
                     SLDEAITAMEHSELAAETLGEHVFDYFLRNKRSEWREYRRQVTEFELGRYLPVL"
     gene            complement(12849..14468)
                     /locus_tag="AW270_RS14425"
     CDS             complement(12849..14468)
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF040097.3"
                     /locus_tag="AW270_RS14425"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_061294804.1"
                     /transl_table=11
                     /translation="MGWLVLAGFWLAAMWALFFFVFPVVFAATVAAAVLGALLAYFVTA
                     GTVLLPDRGVRAPPEGAEPAYRHYLVSQVWRDWWAVLDGSVPMIDERVRKLLRFLNKLL
                     LKDQRRIVLFPVWLAVVAGLVVALLPLAAVAVLVTAAYLVTVVTGLLAWLACVGVLRGA
                     EQSVMLLRRILQACPYPGCYARIDRPVYACPTCQAEHRALRPDRNGAFRHVCRCGARLP
                     TTILLGRHHLRALCPRCGRTLPGKIGKVRVEPVPLVGGPDAGKTTFLAVAVDALRESAG
                     ARFVDQGDEQTFRRLRHQLAAGTMTKTGPQLPKAVMLDVSARILYLFDPSGEHYTGASQ
                     VEAMGYLAHGEAMVLLIDPFALPAVQDSLIPADRELLAAGGVVPSREDPADTVHRVRNE
                     LSTRPDGGLQRRIAVVVTKGDLLGRTTTGQGADDDPRTWLTAMGMGNLVRDLANRGSEV
                     RYATSGLPPRPGEIAGLLTWLTGLNGESRADRPAEEPWRPRSRPEGRPPLSYTVVRRLL
                     FAASALLSAGAVTALILWYVQS"
     gene            complement(14469..16634)
                     /locus_tag="AW270_RS14430"
     CDS             complement(14469..16634)
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF040416.3"
                     /locus_tag="AW270_RS14430"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_061294805.1"
                     /transl_table=11
                     /translation="MAWQLQYTSAESGPTGRAGFQVTAETPGLPPVLRERSLPFLSYQP
                     PPDAPFDDPAALPVSLAYGREVLARCVYLGRDYSGREGNFLGHAVLPGEGELVGLRPIE
                     FWGAPWWADRPSSDASLGSLELAPGPFLDPEALVAWLGGLGPDAYVRLGGLLESVRLFL
                     ARGHGRLVLVSADVAEIVRWIAVVSFSLPWASAVRVSFVTYTGDPASSGQVIAGTTPDV
                     WIPPDIDATVVRLEEPPAPVRTGRFAGTVAGFWRRGDLDGLDALGELGDDPETAAALLA
                     LCRGDDTVSPEEQSAVARLAEGGLPDWAWRRLASQAGLLTHDLASAVAVHAVEGAARCV
                     SLALRDPSLPLPVRLPPPGHASALGAEATAALRAAVTPAQVAGVARVADACDLPVDVAE
                     VESAAARAVPGNQEEAAAELARVPAGWREAVITGYVAGLEAAPGEIGDTLCGLLADRRR
                     DWRRAPRTGVAVLRWQTLHGGRDRVDATVELMDRAFEAFREEALPSLWTAQPTPGEISR
                     LVDLLGPRMDASPTLGELPGRAFVKAGLTDGAALELAERVRDGLPGFPADDAEATLIAA
                     RLPSAPTPVEAATAIDRLTDLTREGNADLAVLTRAAAAKVLATRDPRFRSTVARSVTDA
                     TRRWLIPEWLKPRRTRDQRMALLEIAIRMRAAGVVEPRLDAWARSEVNNWGPFSSVEAR
                     FKQDAELAAGLKELAGQRRRLFGRGER"
     gene            complement(16638..17732)
                     /locus_tag="AW270_RS14435"
     CDS             complement(16638..17732)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018657912.1"
                     /locus_tag="AW270_RS14435"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="GTPase"
                     /protein_id="WP_061294806.1"
                     /transl_table=11
                     /translation="MITCPYCFARVAEDQIAFRCAGGCEPVADEPLGRLRGGTPPSLPP
                     VFRVRRPGRRADCPSCGRDTGRRVCPECHSGLAAEYCANPGRIVALVGAKGSGKSTYIA
                     VLLHELMNRVGAELDASLVPCDDRTIERYRRDFARPLYGERRLLRATQSAVTDTLRDPL
                     VYLLSRTVKGRLRSRKAALTLVLFDTAGEDLRSREVTERHLRYLRAADAVVFLVDPSSL
                     QKPSDDPDSDPVNVIARVTELLRTGKGLLTVPVAVALSKIDAERREPASALHRSRTPSG
                     AFDLDDHEAVHEQVRALLESWQAGGLDRYLRQNYADFAFFALSSLGEAPGDAEVGPGGI
                     RPYRVEDPLLWLLHRFKMLDGVRR"
     gene            complement(17729..20197)
                     /locus_tag="AW270_RS14440"
     CDS             complement(17729..20197)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012852229.1"
                     /locus_tag="AW270_RS14440"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_061294807.1"
                     /transl_table=11
                     /translation="MPFDEARYVREVLDPARAAGAPPDDLLVRYALGRDLAEVGETVRA
                     VRQCWRRQRSQLKFRRLVDRLEADHARLAPLFDAAAGGDPGPLRNALAQAGARERERLE
                     EARRRLDDAAGRLRMVTPDVVAGIARSTGLDLAPLARELGIAVQEPEALASTPPYAAYD
                     RLREALETLRVRHLAAFVLGPEGPYRVLRATSLPLQAVEAEWRRKTRGPWTTAADTLLT
                     ALKSDPAALIRYDMVARLRERVREHPYDDTLLRHAVDDLGLDPAEARRLVFAVRQETGV
                     AGGPAARLRELADAGEIHAAADLAASLSASLELDGPAAELAAEIRARLATAEALRERAL
                     AAEDPDEAWLALADALRRVPDLPGAAELQRRLVPLPAEDVKATPDGASVVVTWRASRSK
                     AGEISYEILRNGSPLDGGEPFRVTDAEPPIDEPVVYGVVARRGVAAAREVAAPPVTVRP
                     EVTDVRVRAGDGLVTGSWTRPAHAAGVVVTRDGTPIEIDGPGFTDRAVRNGVGYVYLVK
                     AVYSGGVTTSGVRARATPCSRPAPVAEFTVEPAPDGRILLRCAPPPAGHVEFRLLESAP
                     PWPFGTTVSVAEARATGRALAVLPAPDGHLVKAASGVLLALTVAGDLATIGGTREHVNL
                     TPPRSLTVERLGRAVHVGLDWPVDVPEVEVAVNGTALVVTAASYRAQGGIRVEVAEDEP
                     VTVEVVPTALVNGVRLRGPGLSRSLEAVPPVRYDLSRSGPFWRRRLIVEVFAERPVTVD
                     RLVLVLRPGRVEPLSPDQGTVLGEWAGVVTPARFEVKAPRQAKPYWLKCFAGDGVELIG
                     PPVRRLKVGT"
ORIGIN
        1 gtcgccgtag agggtcgagt agcccgcggc gagctcgctc ttgttgccgg tggtgaggac
       61 caggtggccg tgctggttcg acagcgacat caggaacatg ccgcggacgc gggcctggag
      121 gttctcggcc gccaggccgg acagctcgat ctccttctcg aacgcggcca ccacgtcggc
      181 gatcggcgcg atccgcgagt ggaggccctg gcggcggacc agctcctcgg cgtcggcgat
      241 cgagtggtcg gagctgtagc gcgagggcat cagcaccacg tggacccggt cggggccgat
      301 ggcgtcggcg gcgatcgtgg cggtcagggc cgagtcgatg ccgcccgaca ggccgaggat
      361 gaccgagcgg aagccgttct tctccacgta gtcgcgcacg ccgagcacga gcgcgccgta
      421 gacctcggcg gggtcgtcga gccgttcggc gatcgtggcg gggcgcggct cgaaggcgac
      481 cgggtcctcc gacaggacca gccgctcgac ggtgatcgag gtgccgtcgc cggcgtcgtg
      541 cacggcctcg ccgggctcgc ccgtgccgag cggcaggtcg aggtcggcga acagcagctc
      601 ctcggtgaac tggcccgcgc gggcgaccag agtgccgtcg gcggccacca cgagggagtc
      661 gccgtcgaag accagttcgt cctggccgcc gacctggttg gcgtaggcga gcgcgcagcc
      721 cgcctcggcc gcccgccgga cgcacaggtc gcgccgcacg tcgtccttct cccgctcgta
      781 cggcgaggcg ttaggcacca ccagcagacc cgccccggcc gctccggcga ccgacaccgg
      841 gccgccctcc tgccacaggt cctcgcagat cgcgacggcc acgtcgacgc cgtggaggcg
      901 gaagaccggc agccggtcgc cgcgcacgaa gtagcggtat tcgtcgaaga cgccgtagtt
      961 gggcaggtgg tgcttggcgg tgcgggccac cacccggccc tggtagagga ccgcggcggc
     1021 gtcgagcggc gcgcccttcg gctggcccac ccgggcgacc aggtcggccc ggtcgacgta
     1081 gccgacgacg accgggaggt cgccgtgccc ggcctcggcg aggcggcggg cggcggactc
     1141 cagggtcgcg atggacgcct cgacgaacga ggcgcgcagg accagatcct cgaccggata
     1201 gccggtcagg aacatctcgg tgaagaccac caggtgggcg ccgccctcgt gggcccggcc
     1261 ggtccattcg atgagccggt cggcgttccc ggcgaggtcg ccgacgacgg gattggtctg
     1321 ggcgagtgcg atgcgaagct gagccacgcc cccaccctac ggtgacgccc cggttttcat
     1381 caatgtgacg ataagcaccc cgggtcaggc gccggggctg acgagcccgc tctcgtaggc
     1441 cagcacgacg agctgggccc ggtcgatcag gtcgagccgc ttgagcagcc cggccacctc
     1501 ggcggcgacc tcggcctcct cgaccgccag ggtgacggcg atctggcggt tggtgaagcc
     1561 gcgcgcgatc agccggacca ggtcgacctc ggagtcgctc agcccgccgg gcgcggcggg
     1621 gttgtggcgg cccgcgaagg ccgcgatgag gtgggtcgtc acggccgggt cgatcagcgc
     1681 gtcgcccgcc acggcggccc ggatcgcggc gatgagctgc tcgggggccg aggtcttcag
     1741 cacgaacccg ctgacccccg cgcgcagcgc ggagtagagg ttgtcgtcgg tgccgaaggt
     1801 ggtcatcatg atcaccttcg gcgggtcggt cccggccagg atccggcggg ccgccgccag
     1861 gccgtcgagg cgcggcatct ggatgtccat cagcacgacg tcgggcctga ccaactggac
     1921 ctgctggatc gcctgctcgc cgttctccgc ctcggccacc atctccaggc cgggctcgct
     1981 gtcgaggacc atcttcaggc cggtgcggac cagggcctgg tcgtcggcca cgaccacccg
     2041 cagcacgtcg gccgggcgcg tccccgtggt ggccggggcc gccgcgaccg ggccgtcgag
     2101 gtgggcggcc tgcccgagga tgtcctgttc cagccgctgg agcgcgggcc ccaggtcgag
     2161 cccgaggtcg tcgcggagca cgtcgcgggc ccggcgcagc gcccccagcg cgtcggcctg
     2221 ccgcccggcc cggtagaggg ccagcgcgag cagccgccag gcctcctcgc gcagcgggtg
     2281 ggcggaggcg tgcgcctcca ggtcggggat gagcgcagcg ctgcggccca gccggacggc
     2341 cgcgtcggcc cgccgctcga ccgcgatcag ccgcaggtcg tcgaggcgct gcgcctcggc
     2401 ctgggcccag ggcaggtcgc cgaactcggc cagcgccggg ccccgccaca gcgccagcgc
     2461 ggtgtcgagc cgggcgaggg tggcctccag gtcgccgccg tcgtcgcgct tcaccaggtg
     2521 ttcgaagtgc caggcgtcca cgtcggcgat ctcggcccgc agggcgtatc cgggcggggc
     2581 cgacacgagc accccggcct cctcgcgggg cgcgcggccg ggctccaggg cccggcgcag
     2641 ccgcgagacg tatccctgga tcgtcgacag ggcctggacg ggaggccgcc ccggccacag
     2701 ctcgtcgatc agcgtgctga ccgggatcgc ccgcccgccc gccaccagca gcctggccag
     2761 cacggcctgc tgccgctgac ctccgagatc gagcggccgc ccgtcggaaa cagcctggaa
     2821 cggccccagg gcccgaagta tcaccatggt gctggtaagc gtagagcaac gtcacatggt
     2881 gccggtcaac ggaaagcggg cggtcacgac ggtgcccccg gtgtcgggcg cggtgatcgc
     2941 gcacgagccg ccgagctcgg ccgcccgctc ccgcatcgag gaggtgccca ccccggcccg
     3001 gcgcacctcg ggcagcccga tcccgtcgtc ggcgatcttg agggtgagca ggtcggcgtc
     3061 cagtcccagc gagatcgaca cccgccgggc gtgggcgtgc cggcgcacgt tggtcagcgc
     3121 ctcctgcgcg atccggtagg ccgccacctc gatcgccgcc gggaggtcgt ccagggggcc
     3181 gtcggccgag acgtgggtgg ccgtgcccgg ttcgagggtc tcggcggcca gggtccggat
     3241 cgcctcggcc aggcccaggt cgtcgagggc gggcgggcgc aggccgtaga cgagctcgcg
     3301 gatgtcgccg gccaccgcgt ccatgccggt gcgcaggtcg tggagcaggt ggtcggcggt
     3361 gccgggggag tccttcatgg tgaggcgggc catgttgagc gtcatcgcca tcgcgccgag
     3421 ggtctggccg agcccgtcgt gcaggtcgcg gcgcagccgc cggcgctcct cctcgcgggc
     3481 cgccaggatg cgctccctgg agcgttgcag gtcgccagcc atccggacgg cgtgggcgac
     3541 gtcggcgacg tagggcagca gggtggcgat gacgcgttcg tcgtgggcgg cgccgaacct
     3601 gcgggtgccg ggcgcgccca ggagcaggcg gccgaccggt tcgccgtgcc agacgagcgg
     3661 gtgctccttg ggggcgtcgc cgacgtcgcc ggaggagacg cgcaggccgt tgacgacctc
     3721 gacccgggcg ccgccgaccg ccaggccgtc gcggaccacg tcgaccacgg cggccagcgc
     3781 ctccgacggg tcggcgttgc ggacctcctc gacgagccgg gcggtcagcg tccgggggtc
     3841 gccgaccggg ccgtagagga cccggtccat gccgcgctgg agcaggcggc gcagcggctg
     3901 gtagaagacg cccgcgaaca gggcggcggc cagaccggcc acgatgtcgt actcggacat
     3961 cagcaggctg aagaccgcgc cggtgccgaa gtagacggcg ctcaccccgg cgatcgtgcc
     4021 cgccacgacg acggtccggc tgaggacgtc gtcgatgccg aacaggccgt gccgcagcac
     4081 cgagaaggtg atcgccaccg ggatcagggc ggtccagaac gtcaggaagg tgaagaaggc
     4141 cgggtcgccg gtgacgacca ggaccacgta gaccgcgaag gtcagcgcgg gccaggcgat
     4201 cgcccggcgc gtctcgggat ccgctccccg catccggacc accatcgaga ccagcgccag
     4261 caggatgcac ccctgctcga cggtccacac cgtgtccgcg agcggccggt agatcgcgcc
     4321 cggcacgaac tccagcgggt tggggatgac tgccgacatg cccgccaccg gcgcgtcggg
     4381 ggtggggcgg ccgacccgga ccgccgtctg gacggcgatg accaccacgg cgaggacccc
     4441 gaacaggctc catccccggg acggccgccg cccgccgatg gcgaacagcg gcaccagcgt
     4501 cgccaggatc cccccgccca gcgaccacgc cgcgacgccc acgacccgga gcacgcccgc
     4561 cgcggccagg tcgccgccgt gctcggcctg caccatgagc gcggagaaca gcacgctggc
     4621 cgcctcgcac agcccaccgg cgatcatcag ccagccgatc ggcaggcgcg ggcgccgggc
     4681 cgcgatgtag gcgcccaggg ccgggaacgt gaccccggcc gtgaagtcgg gcgtcggccg
     4741 gaaggcctcc tggagatgcg cgggaagccg gtaggggatc gccacggcgg cgccgacggc
     4801 ggcgaccgag accacgccga cgacggccgc tatcaccagt gcctggcccg gacctctgtc
     4861 gttcatttcg ggcaggatat tgaggaattg tgactattgg gtgttggtcc ggtgccggat
     4921 cgccggggcg aggaggagct gccccgccgc ggccacgagg cccagcccgg ccgtggtcag
     4981 ccagagcgcc tcgtggccca cttcgagcag gcgggtgccg aggatcgggg tgaccgccgc
     5041 gccgaccgac cacgacagac cgtaaaggcc gttgtagcgg ccccggaggt gcggcggggc
     5101 cagcgcggag gtgatcgcca ggggcatccc ggcggtgagg atctccccgc acgtccagac
     5161 gacgacggcc agggcgtact ggagggtcgt ggtcgcgaag gcctggagcc cgaagccggc
     5221 gcctacgacg gcgaacccgg ccgccagcac caggctcggg ctgaaccggg tcagccaggc
     5281 cgtggtgatc ggctgcacca cgatgatcag cgcgccgttg accgcgatgg ccgcgccgaa
     5341 cgcctccggc ggcagccccc gctgggtcat cgcgatggcg agcgtctgga acgcctgcag
     5401 ataggtgcac gtgtacgcga ggttcatcag gacgaacgcg accatggtcc ggtcgcgcag
     5461 cacgtcgcgg tagccgcccg gcagccgctc cgaggcgggc gggcgggtct ccgggatcag
     5521 ccgccagacc aggtaggcga agaccaggca actggccgcg tcgatccaga acagcagcag
     5581 gaacccgccc gacgcgagcc ttcccccggc caccatggcg accgagaacc ccaggttgat
     5641 cgcccagaag agcagcccgt aggccctcgg ccggtcctgc ggcgacacca cgtcggccag
     5701 cagcgcctgc gacgccgggc ggtagatgtc cacgaccagc cccagcacga acatgccggc
     5761 cgcgatggcg gtcaggtcgt gggcgtaccc gaggaagagc atggtcgccg ccgtcgccag
     5821 catgccgccc gcgagcgtgg cccggcggcc gacccggtcg gccaggaagc ccgcgaccgg
     5881 ctgggacagc agcgacccgc acccccacag ggtcaggacc agaccggtga ccgcgaccga
     5941 gacgccccgc gactgggtca ggtagatccc catgaacggc tgggccatcg tgccgagctt
     6001 gttgacggcg gtgccggaga agaggaccca gaactccctg ggcagcccgc cgacgcggga
     6061 gtggatgaag ccacggacgg gagagcgttc gacggggggc gcggtcatgg tgccccagcc
     6121 tcctgagccg gtccgggccg acgcgaacgt tttagcccgt gctaaaactt gcctggtcac
     6181 cggacggggg agggcaagca tggcggtcga gtggtcgttc acgccgggcg acgtggcgcg
     6241 gctgcggttc gccttctcgc cgatgtggga ggtggtggcc agcctccggg cggtccaggg
     6301 ggcggcgctg catccggcgc agctcccatg gctgcgggag gtgcgcgacc ggctcgccac
     6361 gctcgggctg gaggagctga ccgggctggt gccggcggcg ggatacctgc ccgacttcct
     6421 caccccgacg cccgacaccc accagcccga cttcgccgac gaactcgcgc tcgtccgggc
     6481 cgccgacccc gaggtggcgc ggtccgaggt ggcccggtcg gcgccccgcc gcgccggggt
     6541 ggccgcgcgg ttcgcggcgg atcccgccgg cgcgctcgcc tggacggggg acgcgctgga
     6601 ggcgtactgg aacctggtct tcgcggagtt ctggccgaag gtgcgcgggg tgctggaggc
     6661 cgacgtgctc taccggtcgc ggcggctgac cgagggcggg gtgagcgacc tgttcgcggc
     6721 gctgcacccg tcggtgacct ggcacggcga ccggctgatc acccacaagg actgcatcca
     6781 cctggccgag ctcggcggcg cggggctggt gctgctcccg agcgtgttcc agcggctcga
     6841 ccgggtcgcg atcgtgcacg agccctaccg gcccatgctg atctatccgg cccgggggac
     6901 ggggaacctg tgggcgcgcg gccccgagga ggtgcccggc gcgctgtcgg ccctgatcgg
     6961 gcggacccgg gcgcgcatcc tgaccgcgct ggagaccccg gcgtcgacgt cggggctggc
     7021 cagggtgatg gaactgacgc cgggggctgt ttcgcagcac ctcaccgtcc tgtcggacgg
     7081 aggcctggtg tcccggcagc gcctcggacg cgaagtcttc taccagcgga ccccgatcgg
     7141 cgacgccctg gtgggacggc cctagaccgc ccgtcgcggc ggggctccgc gcggccggtc
     7201 ggcgccgggt gcggcgaggg ccggaagcgg cgggcggcgc cggggcaggc ggagccgtaa
     7261 cggaagaagg gctcttacag tgggattctg gcggtgatcg tggtgccctc acccgaggtg
     7321 ccgatctcgc agccgccgcc gagttcggcg gcccgctccc gcatcgacga caggcccgcc
     7381 tccgggcggc cgccggcgcg gatctccacg accaggtcgg cgccccggct gacggtgatc
     7441 gccgtcgcgg cggtgtggcg gagggcgtcc tgggcgatgc ggtaggcggc cacctcgacg
     7501 gcggcgggca ggccggtcag gtcgccctcg acgacgaggt cgatggggcc gagctgcctg
     7561 atcgcgccgg ccaggccgag gtcgtcgagg gcgggcgggc gcaggccgtg gacgagttcg
     7621 cggatgtcgc cggagacggc gtccatgccg ccgcgcaggt cggccagcag ggtgtcggcg
     7681 atctcggggc tgcccctgag ctgggtccgg gccatcgaga tgcgcatcgc catcgccgac
     7741 agggtctggc cgagcccgtc gtgcaggtcg cggcgcaggc ggcggcgctc ctcctcgcgg
     7801 gcggtcagga tgcgctcccg cgaccgccgc agcgccgtgg tcagccgcat cgtgtgggcg
     7861 gcgtcggcga tgtacggggt caggaccgcg atcacccgct cgtcgtgggc ggccgggaag
     7921 cggcggggcc ccggcgggcc gatcagcagg gtgccgaccg gctcgccgtg ccagaccagc
     7981 tcgacccggc gggccaccac gggcagctcg ccccacgagg tgcgggtgcc gtcgaggtcg
     8041 agcgcggtgc cggtcaccgc caggccctcc ttgatcgtct ccagggtggc cagcaggccg
     8101 tggacgggat cggcctgctg gagccgtccg cgcaggtcgt cggcgagggt gaccgggtcg
     8161 ccgcgggtgc cgtagagggc ccggtcggcc agccggtgga gggcccgcct catcgggtgg
     8221 aagaacaccc ccgccccgag ggccgcgacc agtccgaaat agcggtcgac ctcggccagg
     8281 aacaggctgg aggccgtccc tgccagcacg tacaccgacg ccaccacggt caccatcccg
     8341 gcgcccacca gcgcccgggt gatcagggtg tcgatgccga acagccggta gcgcagcacc
     8401 gcgagcgtga tcgccgccgg gatcagcggg atcgtgatgg tggtgaccca ccacaccgag
     8461 ctgccgatga tccacggcac gaccaggcag gagatggcca gcagcggcca gaggacctgc
     8521 cgccgctcgt cctccccggc ccgccggaac cgcagcacca gcgacgagaa cgcgatcacc
     8581 agcgacagcg tgacgagccc ctggagggtt cgcccggccc acgacggccc cggcccggcg
     8641 atcgcggcga gccattcggc ggccagcacc gccgccgcga acccgcccgg ccagcaccac
     8701 gcccgcgaca gcagcgcccc ggtcggatag agcatcggca gcagcacgcc cagggcgtag
     8761 gtcgtcaggt tccagacgcc gtgggagaac acgtcgagcg gcgccgccag cggatggccg
     8821 ccctcccgga ccagcagcgc cgccaggtcg gcggcggcga cgctggccgc cgcgcccagc
     8881 ccgcccagcg ccagcagcca cccgatcacc agcttcggcc gccgctgcac cagcagcgcc
     8941 ccgaacaccg ggaacgccag ccccatcacg tcctccggcg tgaacggcag cggggcgccg
     9001 atcacgaggt cggcgacgac cagcacgacc gccgcgcccg cgatgcccgc cgccagcacc
     9061 cgcgccgccc ggtcgggcac ccgcatccat cgcggccggg tctccgcctc gggagaagcg
     9121 ccggtcatca gggtccgcct tacgagtcgt ccgagtcctt ctggctctct gagacggggc
     9181 ccggtgaggg aaagttcaac agatatccgg cgattcgggt gacggtctgt cagaaggggt
     9241 ggtgatggtc cggaccaccg gggggaccac gccgtagagg cgctcccccg accgctgggt
     9301 cgcgccgggc cgcagccgga tcccggtgac ggtggcctgg ccgccgtaga cgtccgagac
     9361 cctgatcgtg tacggccccg ggcccggctc cggcgggaac acccagtagt tgtcggcccc
     9421 ccggcgcaga tcccgccagt ggacgccgtt cttgatccgc acccgcgcca gccggttgcc
     9481 gtggtcgagc acctgcaccg cgctccagtg ggcggtcgag cccggtttga cgcggaaggc
     9541 cagccgccgc cccggctccg ggtcgcggac ccgccggtag gtgatcgccg cgctgccgtc
     9601 gtcccggtcg gcgatgctga cgaacgcctc cgccgacagg tcgaggtcac ccgggccgca
     9661 ggccgggcag cggtcgacga ccagcacccg cacagtcccg gccggcccgg tcacgtcgag
     9721 atagccgccg caggcggccg acccgtcgta ctccggcgtc gacaccgagg cgaacagcat
     9781 ggggtggccg ggcagcgagc agttgcccgg cgccttcgcc tcgtagaagt aggcccgccc
     9841 ccgcctctcc ggcgaggcgc agaccggacc ggtcagctgg acgaggaccg ccgccgcgac
     9901 gagcgccgcg acggcccaca ccactccgcg gcgcctgcgg cgcctcgcgt gtctcatgcc
     9961 ggatacatag agacacgcgg tgattacgtt acgtagatct tcactggtag cgcggggact
    10021 ccagcagcca ttcgaggtag gccctcggca ccccgaacag ggccgcggcc accttctcga
    10081 cgtcgtcggg gaccccgagc tccgcgtacg actccggcag cgccttggcc gcgtcggtca
    10141 ggtcctcggc ccgctccacg atccggtcgg ccacccactc ggccggggcg ggcaccccga
    10201 tccgcgacac cgccacccgc acgtagttgt gcgggtcgcc gcgctccttg ccgagcgagg
    10261 ccacgtcgtt gcaccaggcc gtcacgtcga ccacggcgtc gaggagggtc cgccacggcg
    10321 gcgtggcgtg cagccgtccg ggcacctcga tccgcaggca cccctcgatg atctccgcgc
    10381 cggcgcccgc gaagacgtcc cgccgcagcg ccgggtactc cccggccgag gggacccgcc
    10441 cgatcgaccg gttcaccgcc tgcatccggc acgcctgcgc ctgcgcctcg atccgctgcg
    10501 tgaaccgctg ctcccaggcg ggcgacatgt cgggcgcggt cacccgccac aggtcggcga
    10561 acgccgccac cagcggctcc tcggtccggt gaccggcgtg ggcggcctcc cgcaggcggc
    10621 cctgcatcag atcgagcacg gtggtgtccc ggctgccgct gccctcgtcg caccagtcgt
    10681 cgtaggcgag ctgccaggtg acccaccggg cgagcagctc gttccccggc agcacccgcg
    10741 cggccaggcg ctcggggcgg gcggcgatcg agaggccgta cagccccgcc caggccccca
    10801 gcgcccgggt gtcgcacacg accggttctc ccacggtcgg cggcagacag tagacgagcg
    10861 gggccagaac ggcggcagcc gaaggtatgc gcagctcagg ggtcatgtga tgacacgcta
    10921 ggtgagcatt caccggctgt cccgcaggcg cgacggggag tcctcacggc aggtgcgtaa
    10981 cgtatgtgaa acacacaagg ggcacactgg taggtgtcca gacctggaac tttcgtgagg
    11041 tagtcgcctt ggaccgccag caggaattcg ttctccgcac gctcgaagag cgcgacatcc
    11101 ggttcatccg gctctggttc accgacgtcc tcggcttcct caagtccgtg gccatcgcgc
    11161 cggccgaact ggagggcgcc ttcgccgagg gaatcggctt cgacggctcc gccatcgagg
    11221 gcttctcccg cgtgtacgag tcggacatgc tcgccaagcc cgacccgtcg accttccaga
    11281 tcctgccgtg gcgcagcgag acccccggcg cggccaggat gttctgcgac atcctcatgc
    11341 ccgacggctc cccgtcccac gccgaccccc gctgggtcct gaagcggacc ctggcgcgcg
    11401 ccgccgacat ggggttcacc ttctacaccc accccgaggt cgagttcttc ctcctccgcg
    11461 accgccccga gcgcggcgtc cggcccgagc cgatcgacga cggcggctac ttcgaccaca
    11521 ccccccacag cgccggccac gacttccgcc gcaacgcgat catgatgctg gaggcgatgg
    11581 ggatctcggt ggagttcagc caccacgagg gcgcccccgg ccagcaggag atagacctcc
    11641 gctacgccga cgccctgtcg accgccgaca acatcatgac cttccggctc gtgatgaagg
    11701 aggtggcgct ggagcagggc gtctgggcct ccttcatgcc caagccgttc accgagtacc
    11761 ccgggtcggg catgcacacc cacatgtccc tgttcgaggg cgaccgcaac gccttctacg
    11821 agcccgggtc ggactaccag ctctccaagg tgggccggtc gttcatcggc ggcctcctca
    11881 agcacgccgc cgagatcacc gcggtgtgta accagtgggt gaactcttac aagcgcctct
    11941 ggggcgacac cgaggccatc gccggggcgg gcggcgaggc tccctcgtac atctgctggg
    12001 gccacaacaa ccggtcggcg ctggtccggg tgccgatgta caagccccac aagggtggct
    12061 cgacccgcat cgagttccgc tcactcgact cggcctgcaa cccctacctg gcgtacgcga
    12121 tgatcctggc ggcggggctg aaggggatcg aagagggcta cgacatcccg gccggagccg
    12181 aggacgacgt gtgggcgctg acgtcgggcg agcggcgggc actgggaatc cacccgctgc
    12241 cgcagtcgct cgacgaggcg atcacggcga tggagcacag cgaactcgcg gcggagacgc
    12301 tgggggagca cgtgttcgac tacttcctgc gcaacaagcg gtcggagtgg cgcgagtacc
    12361 ggcgccaggt gaccgagttc gagctgggac ggtacctgcc ggtcctgtag gacttacgac
    12421 ccccggcagg gcaggatcac gcccgccggg ggtttttcat gcgccggccc cgccgggcag
    12481 cgttcccggc gatcgctccc tgagactgtg ccgccgtaca ggtcgcctgt gagggttcca
    12541 ctgcggcgga ctttagttga ctaaagcttc agggggctgt gcgtgagcgc ggacagctcg
    12601 cccgatgctg gcttctgcat cgctccaaac agctacatgt aacttacttc agtgttcgga
    12661 ctttagctga ctattcaagg atcatgtact cgtatttacc attgcgaagt aatcgaacga
    12721 aggggacagc agtgggcgca atggtggggc tcgcgatcgg gctcgcggtg ataggtgtac
    12781 cggtgatcat cgtcgtgatc gtgatggcgg tggtcgcggc gatcaagagg gactgatccc
    12841 ggcgcagatc aggactggac gtaccagagg atcaacgcgg tcaccgctcc tgcgctcagc
    12901 agggcgctgg ctgcgaacag caaccgacgg accaccgtgt aactcagtgg ggggcggccc
    12961 tcgggacggg accggggacg ccagggttcc tcggccggcc ggtcggcccg gctctcgccg
    13021 ttcaggccag tcagccacgt cagcaagccc gcgatctccc ccggcctggg cggcagcccc
    13081 gacgtcgcgt agcggacctc cgacccccgg ttggccaggt cccgcaccag gttccccatc
    13141 cccatcgccg tcagccacgt ccgggggtcg tcgtcggcgc cctgcccggt ggtcgtccgg
    13201 ccgaggaggt cgcccttggt caccaccacc gcgatccgcc gctggagtcc cccgtccggg
    13261 cgggtggaga gctcgttgcg gacccggtgc accgtgtccg ccgggtcctc gcgcgagggg
    13321 accacgcccc ccgccgccag gagctcccgg tcggccggga tcaggctgtc ctggacggcc
    13381 ggcagcgcga acgggtcgat cagcaggacc atcgcctcgc cgtgggccag gtagcccatc
    13441 gcctcgacct ggctggcccc cgtgtagtgc tccccggagg ggtcgaacag gtacaggatc
    13501 cgggccgaca cgtcgagcat gaccgccttc ggcagctgcg gcccggtctt ggtcatcgtt
    13561 ccggcggcca gctggtggcg caggcggcgg aaggtctgct cgtcgccctg gtcgacgaac
    13621 cgcgccccgg cgctctcccg cagcgcgtcg acggccacgg ccaggaacgt ggtctttccc
    13681 gcgtcggggc cgccgaccag cgggaccggc tccacccgca ccttgccgat cttcccgggc
    13741 agggtccggc cgcagcgggg gcacagcgcc cgcaggtggt ggcggcccag caggatcgtg
    13801 gtgggcaggc gcgcgccgca gcggcagacg tgccggaacg cgccgttgcg gtcggggcgc
    13861 agggcgcggt gctccgcctg gcatgtcggg cacgcgtaga cgggtcggtc gatccgcgcg
    13921 tagcagccgg gataggggca ggcctggagg atccgccgca gcagcatcac cgactgctcg
    13981 gcgccgcgca ggacgccgac gcacgccagc caggccagca gcccggtcac caccgtcacc
    14041 aggtaggcgg cggtgaccag gacggccacc gcggccaggg gcagcagcgc cacgaccaga
    14101 ccggccacca cggccagcca caccgggaag agcacgatgc gccgctggtc cttcaggagc
    14161 agcttgttca ggaatctcag gagcttgcgg acccgctcgt cgatcatcgg gaccgagccg
    14221 tcgagcaccg cccaccagtc gcgccagacc tgcgagacca ggtagtgccg gtaggcgggt
    14281 tcagcccctt cggggggcgc ccgcacccct cggtccggca ggagcaccgt ccccgccgtc
    14341 acgaaatagg ccagcaacgc cccgaggacc gccgccgcga ccgtcgccgc gaacaccacg
    14401 gggaagacga agaagaacag cgcccacatg gccgcgagcc agaacccggc gaggacgagc
    14461 catcccatct accgctctcc gcgcccgaac agccgtcggc gctgcccggc cagctccttc
    14521 agaccggcgg ccagttccgc gtcctgcttg aagcgggcct cgaccgagct gaacgggccc
    14581 cagttgttga cctcggagcg ggcccaggcg tccaggcggg gttcgaccac cccggcggcg
    14641 cgcatcctga tggcgatctc cagcagcgcc atccgctggt cgcgggtccg gcggggcttg
    14701 agccactcgg ggatgagcca gcgccgcgtc gcgtcggtga ccgacctcgc gacggtcgag
    14761 cggaagcggg ggtcgcgggt ggccaggacc ttggccgcgg cggcgcgggt caggacggcc
    14821 aggtcggcgt tgccctcgcg ggtcaggtcg gtcagccggt cgatcgccgt ggcggcctcc
    14881 accggggtcg gggccgacgg gaggcgggcc gcgatcaggg tggcctcggc gtcgtcggcg
    14941 gggaagccgg gcaggccgtc gcggacgcgt tcggccagct ccagggccgc cccatccgtg
    15001 aggcccgcct tcacgaaggc gcggccgggg agttcgccca gcgtggggct ggcgtccatg
    15061 cgcgggccga gcaggtcgac cagccgggag atctcgcccg gcgtgggctg ggcggtccac
    15121 agcgacggga gcgcctcctc gcggaaggcc tcgaacgcgc ggtccatgag ctcgaccgtc
    15181 gcgtcgaccc ggtcgcgtcc gccgtggagg gtctgccagc gcagcaccgc cacccccgtc
    15241 cggggggcgc ggcgccagtc gcggcgccgg tcggcgagca ggccgcagag ggtgtcgccg
    15301 atctcgccgg gggccgcctc cagcccggcg acgtacccgg tgatcacggc ctcccgccag
    15361 cccgccggga cccgggccag ttcggccgcg gcctcctcct ggttgccggg gacggcccgc
    15421 gccgccgccg actcgacctc ggccacgtcg accggcaggt cgcacgcgtc ggccacccgc
    15481 gcgaccccgg ccacctgggc gggggtgacg gcggcccgga gcgcggcggt cgcctccgct
    15541 ccgagcgcgg aggcgtggcc ggggggcggg agccgtaccg gcagaggaag tgaggggtcc
    15601 cgcagggcga gcgacacgca ccgggccgcg ccctcgacgg cgtggacggc caccgccgac
    15661 gccaggtcat gggtgagcag ccccgcctgg gacgccagcc gccgccacgc ccagtcgggc
    15721 agcccgccct cggcaagccg ggcgacggcg ctctgctcct cgggcgagac cgtgtcgtcg
    15781 ccccggcaga gcgcgagcag cgccgccgcc gtctcggggt cgtcgcccag ttcgccgagc
    15841 gcgtcgaggc cgtcgaggtc gccccgccgc cagaacccgg ccacggtccc ggcgaaccga
    15901 ccggtccgca ccggggccgg gggctcctcc agccggacca cggtcgcgtc gatgtccggg
    15961 gggatccaca cgtcgggggt ggttcccgcg atgacctgcc cggacgaggc cgggtcgccg
    16021 gtgtaggtga cgaaggagac ccggaccgcc gacgcccacg gcagcgagaa cgagaccacc
    16081 gcgatccacc gcacgatctc cgccacgtcg gcgctgacca gcaccagccg cccgtgcccc
    16141 cgggccagga acagccgcac cgactccagc agcccgccga gccgcacgta cgcgtcggga
    16201 cccagcccgc ccagccacgc caccagggcc tcggggtcga ggaagggccc gggggcgagc
    16261 tccaggctcc ccagcgacgc gtcggaggag ggccggtcgg cccaccacgg cgcgccccag
    16321 aactcgatcg gccgcagccc gacgagctcc ccctcgcccg gcagcaccgc gtggccgagg
    16381 aagttgccct cacgtcccga gtagtcccgg cccaggtaga cgcaccgggc cagcacctca
    16441 cgcccgtagg ccagcgagac cggcagggcg gcggggtcgt cgaaaggggc gtccggggga
    16501 ggctggtagc tcaggaaggg aaggctccgc tcgcgcagca ccggcggcag gccgggggtc
    16561 tcggccgtga cctggaaccc cgcccgcccg gtggggcccg attcggccga cgtgtactgg
    16621 agctgccagg ccatgaccta cctccgcacg ccgtcgagca tcttgaaccg gtggaggagc
    16681 cacagcaggg gatcctccac ccggtagggg cggataccgc ccggtccgac ctccgcgtcc
    16741 cccggcgcct cgcccaggga cgacagcgcg aagaacgcga agtcggcgta gttctgccgc
    16801 agatagcggt cgagcccgcc ggcctgccag ctctccagca gcgccctgac ctgctcgtgc
    16861 accgcctcgt ggtcgtcgag gtcgaaggcg cccgaggggg tccgcgaccg gtgcagcgcg
    16921 gaggccggct cccgccgctc cgcgtcgatc ttgctgagcg cgaccgccac ggggaccgtc
    16981 agaagccctt ttccggtacg cagcagctcc gtcacccggg cgatcacgtt caccgggtcg
    17041 ctgtcggggt cgtccgacgg cttctgcaag gacgaggggt cgaccaggaa caccaccgcg
    17101 tcggcggccc gcaggtagcg caggtgccgt tcggtcacct cgcggctgcg caggtcctcg
    17161 cccgccgtgt cgaacagcac cagcgtcagc gccgccttcc ggctgcgcag ccggcccttc
    17221 acggtccggc tgagcaggta gacgaggggg tcgcgcaggg tgtcggtgac cgccgactgg
    17281 gtcgcccgca gcagccgccg ctccccgtag agggggcggg cgaagtcgcg gcggtagcgc
    17341 tcgatcgtcc ggtcgtcgca cggcaccagc gacgcgtcga gctcggcgcc gacccggttc
    17401 atcagctcgt gcagcagcac cgcgatgtac gtgctcttgc ccgacccctt cgcccccacg
    17461 agggcgacga tccggccggg gttggcgcag tattcggcgg ccagcccgct gtggcactcg
    17521 gggcagaccc gccgcccggt gtcccggccg cacgaggggc agtcggcccg ccgccccggc
    17581 cgccgcaccc ggaagaccgg cggcagcgac gggggagtgc cgccgcgcag ccgtccgagg
    17641 ggctcgtcgg ccaccggttc gcagcccccg gcgcaccgga acgcgatctg gtcctcggcc
    17701 acccgggcga agcagtaggg gcaggtgatc atgtgcccac cttgagccgc cgcaccggcg
    17761 gcccgatcag ctcgacgccg tccccggcga agcacttgag ccagtacggc ttggcctgcc
    17821 ggggcgcctt cacctcgaac cgggccggcg tcaccacccc ggcccactcg ccgagcaccg
    17881 tcccctggtc cggcgacagc ggctccaccc ggcccggccg cagcaccagg accagccggt
    17941 cgaccgtgac cggccgttcg gcgaagacct cgacgatcag gcgccgccgc cagaacggcc
    18001 cgctccggga caggtcgtag cggaccggcg gcaccgcctc cagcgaccgc gacaggccgg
    18061 gcccgcgcag ccggaccccg ttcaccaagg ccgtcggcac cacctcgacc gtgaccggtt
    18121 cgtcctcggc cacctcgacc cggatcccgc cctgcgcccg gtaggaggcc gcggtgacga
    18181 ccagggccgt gccgttcacg gccacctcga cctcgggcac gtcgaccggc cagtcgagcc
    18241 cgacgtgcac ggcccgcccg aggcgttcga cggtcagcga cctcggcggg gtcaggttga
    18301 cgtgctcgcg ggtgccgccg atcgtggcca ggtcgcccgc gacggtcagc gccagcagca
    18361 cccccgacgc cgccttgacg aggtgaccgt cgggcgcggg cagcaccgcc agggcgcggc
    18421 cggtggcgcg ggcctcggcc accgagaccg tcgtgccgaa gggccacggg ggagccgact
    18481 ccagcagccg gaactccacg tgaccggcgg ggggaggcgc gcagcgcagc aggatccggc
    18541 cgtcgggggc gggttcgacg gtgaactcgg ccaccggcgc ggggcgggag cagggggtgg
    18601 cgcgggcgcg caccccggag gtcgtcacgc cacccgaata gaccgctttg accaggtaga
    18661 cgtagccgac gccgttgcgt accgcccggt cggtgaaacc gggaccgtcg atctcgatgg
    18721 gggtcccgtc gcgggtcacc accaccccgg cggcgtgggc ggggcgcgtc cagctcccgg
    18781 tgacgaggcc gtcgcccgcc cggacccgca cgtcggtcac ctcggggcgg accgtcaccg
    18841 gcggcgcggc cacctcgcgg gcggcggcga cgccgcggcg ggccaccacg ccgtagacga
    18901 ccggctcgtc gatcggcggc tcggcgtcgg tcacgcggaa cggctcgccg ccgtcgaggg
    18961 gagaaccgtt tctcaggatc tcgtacgaga tctcgcccgc cttcgacctc gacgcccgcc
    19021 acgtgaccac cacggaagcg ccgtcgggcg tggccttcac gtcctcggcg ggcagcggga
    19081 ccagccgccg ctgcaactcc gccgcaccgg gcaggtcggg cacccgccgc agggcgtcgg
    19141 ccagcgccag ccacgcctcg tcggggtcct cggcggccag cgcccgctcc cgcagcgcct
    19201 cggccgtggc cagccgggcc cggatctcgg cggccagctc cgccgccgga ccgtccagtt
    19261 ccagagaggc ggacagggag gcggccagat cggcggcggc gtggatctcc ccggcgtcgg
    19321 ccagctcccg cagccgcgcg gcgggcccgc ccgcgacccc ggtctcctgc cggaccgcga
    19381 acaccagccg ccgcgcctcg gcgggatcga gccccaggtc gtcgacggca tggcggagga
    19441 gggtgtcgtc gtaggggtgc tcccggacgc gttcgcgcag ccgggccacc atgtcgtacc
    19501 ggatcagcgc ggccggatcg gacttcagcg cggtcagcag cgtgtcggcc gccgtcgtcc
    19561 acggcccccg cgtcttccgc cgccactcgg cctcgaccgc ctgaagaggc aggctggtcg
    19621 cgcgcagcac ccggtagggc ccctcgggtc ccaggacgaa agcggccaga tgccgcaccc
    19681 gcagcgtctc cagcgcctcg cgcagccggt cgtaggcggc gtaagggggc gtcgacgcca
    19741 gcgcctcggg ctcctggacc gcgatcccca gctcacgggc cagcggcgcg aggtcgagcc
    19801 cggtcgagcg ggcgatcccg gccaccacgt cgggcgtgac catccgcagc cgccccgccg
    19861 cgtcgtcgag ccgccgccgg gcctcctcca gacgctcccg ttcgcgcgcc ccggcctgcg
    19921 ccagcgcgtt cctcaacggc ccggggtcgc ccccggcggc cgcgtcgaag aggggggcca
    19981 gccgggcgtg gtcggcttcg agccggtcga cgagccgccg gaacttgagc tggctccgct
    20041 gccgccgcca gcactgccgg accgccctga ccgtctcccc gacctcggcc aggtcgcggc
    20101 ccagggcgta gcggaccagg agatcgtcgg gaggggcccc ggccgcgcgc gccgggtcga
    20161 gcacctcccg cacatagcgg gcctcgtcga acggcatccc gggatcagct gaccgtcagg
    20221 tgcgacaggc tcgcgcgcga ctcggcgacg tcctcctccg tcatcccgcc gatctgcacg
    20281 ttcaggctca gctcctgacc ggtctcgtgc tcgcgcgcgg tgacgtggag cagcccggag
    20341 gtgtcgaggg tgaaggtcac ctcgatcggg aacccggccg gtttccccgg cgggatcgcc
    20401 aggtcgccct cggcgatccg gttgttgtcc tcgaccgcgt ccgacgcctc cgcgcccgcc
    20461 tgctccatca cctcgatggc cacccgggtc tggtagtcgt cgagcgtcgc gaacccctcg
    20521 gtcacgtcgg cgggcagcgg gtcgttggcc cgcaccaggt gggcgacgta ctcgcgccgg
    20581 gtgttgaccg agacggcctt gatcccgaag gcccgcgagg cgaccgtctt gatcgtccgc
    20641 ccggcgatgc gctgctgctc gcccgagctc aggcccgcgc gggcggccat ctcctcggcc
    20701 tgctcgcggg ctccgcccgc caggagccgc cggtacgtct cctcgaaggc gtacagggcc
    20761 gcgcccttgg ccaccgcgag gtcggggtcc tgcaaccggg gctgcacgcc gagctccttg
    20821 gccagcgtct cggccacggc cggcatcttc gacgagccgc ccaccagcac gagatggtcg
    20881 aacgactcga cccccttggc gcgggc
//
