LOCUS       NZ_BBXG01000005        11914 bp    DNA     linear   CON 08-AUG-2024
DEFINITION  Herbidospora cretacea strain NBRC 15474, whole genome shotgun
            sequence.
ACCESSION   NZ_BBXG01000005
VERSION     NZ_BBXG01000005.1
KEYWORDS    WGS; HIGH_QUALITY_DRAFT; RefSeq.
SOURCE      Herbidospora cretacea
  ORGANISM  Herbidospora cretacea
            Bacteria; Actinomycetota; Actinomycetes; Streptosporangiales;
            Streptosporangiaceae; Herbidospora.
REFERENCE   1
  AUTHORS   Komaki,H., Ichikawa,N., Oguchi,A., Hamada,M., Tamura,T. and
            Fujita,N.
  TITLE     Genome-based analysis of non-ribosomal peptide synthetase and type-I
            polyketide synthase gene clusters in all type strains of the genus
            Herbidospora
  JOURNAL   BMC Res Notes 8, 548 (2015)
   PUBMED   26452464
  REMARK    DOI:10.1186/s13104-015-1526-9 Publication Status: Online-Only
REFERENCE   2  (bases 1 to 383282)
  AUTHORS   Hosoyama,A., Oguchi,A., Ichikawa,N., Kimura,A., Ohshita,R. and
            Fujita,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAY-2015) Contact:Director-General Department Of
            Biotechnology National Institute of Technology and Evaluation,
            Biological Resource Center (NBRC); 2-49-10 Nishihara, Shibuya-ku,
            Tokyo 151-0066, Japan URL :http://www.nite.go.jp/nbrc/index.html
COMMENT     ##Genome-Assembly-Data-START##
            Finishing Goal                    :: Noncontiguous Finished
            Current Finishing Status          :: High-Quality Draft
            Assembly Method                   :: newbler v. 2.6
            Genome Coverage                   :: 71x
            Sequencing Technology             :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_001570605.1-RS_2024_08_08
            Annotation Date                   :: 08/08/2024 02:39:29
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,566
            CDSs (total)                      :: 7,487
            Genes (coding)                    :: 7,430
            CDSs (with protein)               :: 7,430
            Genes (RNA)                       :: 79
            rRNAs                             :: 4, 5 (5S, 16S)
            partial rRNAs                     :: 4, 5 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 10 of 57
            Pseudo Genes (incomplete)         :: 49 of 57
            Pseudo Genes (internal stop)      :: 3 of 57
            Pseudo Genes (multiple problems)  :: 4 of 57
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 12:10:14
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 348192
            Orig. end                         :: 360106
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            BBXG01000005.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..11914
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="3"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..11914
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..11914
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:6914](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..6914
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     misc_feature    192..205
                     /note="TFBS match to CatR, H2O2-responsive repressor,
                     confidence: medium, score: 18.36"
                     /tool="antismash"
     gene            complement(307..1905)
                     /locus_tag="AW270_RS12605"
     CDS             complement(307..1905)
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1068:
                     extracellular solute-binding protein family 5"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013130574.1"
                     /locus_tag="AW270_RS12605"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="WP_061294135.1"
                     /transl_table=11
                     /translation="MKPFRVRFARLAPLVLIASLALGACSAPTVPQTSSSGSADDTFVL
                     GIPSEPATLNPVLGYAVDGGGLMFDGLLSRNADLSLKPALADAMPATDGETVTFTLRKD
                     VKFHDGTPLTSADVAYTYQAVMDEKNNSSIRGDFAAIESVDAPDASTVVFHLKYPYAAI
                     LQRATLGIVPKKLLDGQSLESAPFNTKPVGTGPYSFVSWTPGDKLVVKANPTYWGGAPA
                     IKNLVVAYAGDDNVRATRMAAGEFDATVLPPKAAARFDGQQGLTVHKVDSADYRGVMFP
                     FGQPVTGDLAIRKAVNLALDRQAMVDTILAGAGEPAYGPIAPGTTWYNAAIAGSPAPDQ
                     AQATKVLDEAGWKVGDDGVRVKDGTRASFTLMYPAGDALRKDLGLAVASDAKKVGIDIE
                     LAGLDWDAIEPRMSKDALVMGYGAPYDPDYTNYELFHSTLAGQGFFNPGFYKVPEVDAL
                     LDKGREEADPAVRKQTYDAFQQRISDDAAWAYLVYLKHVYVVRGQWTGIAPSVEPHEHE
                     TGGLFRNIAEWKPVA"
     misc_feature    complement(1935..1953)
                     /note="TFBS match to FuR_variant_2, Ferric Uptake
                     Regulator, confidence: weak, score: 24.12"
                     /tool="antismash"
     misc_feature    complement(1936..1952)
                     /note="TFBS match to NuR, Nickel-responsive regulator,
                     confidence: weak, score: 25.46"
                     /tool="antismash"
     gene            complement(2072..2488)
                     /locus_tag="AW270_RS12610"
     CDS             complement(2072..2488)
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1266: hydrogen
                     peroxide sensitive repressor"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: protein motif:HMM:NF013629.4"
                     /locus_tag="AW270_RS12610"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="Fur family transcriptional regulator"
                     /protein_id="WP_061294138.1"
                     /transl_table=11
                     /translation="MDRIETDQMLADRGLRRTGARMAILTILERTNTHHSVSTLAQLAA
                     AVRPTINASSVYRNVTAMTESGVLHCIEWAGELLYGLAITPHHHLICQPCGTLLEIPAG
                     DLDRTAADLATGRRFTLNQAGQLLLGRCHQCSAA"
     misc_feature    2514..2532
                     /note="TFBS match to FuR_variant_2, Ferric Uptake
                     Regulator, confidence: weak, score: 22.64"
                     /tool="antismash"
     misc_feature    complement(2515..2531)
                     /note="TFBS match to NuR, Nickel-responsive regulator,
                     confidence: weak, score: 24.53"
                     /tool="antismash"
     gene            2544..3347
                     /locus_tag="AW270_RS12615"
     CDS             2544..3347
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0008270 - zinc ion binding [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF017277.4"
                     /locus_tag="AW270_RS12615"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="MucR family transcriptional regulator"
                     /protein_id="WP_061294140.1"
                     /transl_table=11
                     /translation="MDGDVHGRIGELACDEVTGEVQCHLCGRSFRALGSHIRVHDLTAD
                     AYREAFGLFATKALTSQELSETRRDRQQRLYRRSPATRSNLGTGQKLARSGELNTLTRR
                     DSPQRRTSQLRELKDGRATRSRTTQERLRTALADAGFPDSAAGLRTLYVDRQTSMENLA
                     ALLGVSRVTLRNALLAADIPLRAIGVNTDTGRRSRIASNIARAAALVGTTDLHQWLHER
                     RSQGASLQQLATELNRSVPWVKDRLSERSDHEGRARSSRPAVPPG"
     gene            complement(3467..4066)
                     /locus_tag="AW270_RS12620"
     CDS             complement(3467..4066)
                     /EC_number="1.15.1.1"
                     /GO_function="GO:0004784 - superoxide dismutase activity
                     [Evidence IEA]"
                     /GO_function="GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018653991.1"
                     /locus_tag="AW270_RS12620"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="superoxide dismutase"
                     /protein_id="WP_061294142.1"
                     /transl_table=11
                     /translation="MSQYTLPDMPYDYAALEPAITGEILELHHSKHHAAYVKGANDTLD
                     RLAEVRDKGDFSGLVGLEKTYAFNLSGHVLHTIFWQNLSPDGGDRPDGELGDSIVEHFG
                     SFEAFQKQLTTATATVQGSGWGVLAWEPLGRRLIVEQVYDHHGNVGMSSTPLLVFDAWE
                     HAYYLQYKNVRPDYVEKLWSLINWNDVIARFDAARA"
     misc_feature    4083..4099
                     /note="TFBS match to NuR, Nickel-responsive regulator,
                     confidence: weak, score: 20.66"
                     /tool="antismash"
     gene            complement(4144..4617)
                     /locus_tag="AW270_RS39515"
     CDS             complement(4144..4617)
                     /EC_number="2.3.1.-"
                     /GO_function="GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /locus_tag="AW270_RS39515"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="N-acetyltransferase"
                     /protein_id="WP_061294143.1"
                     /transl_table=11
                     /translation="MKSSDLWSLLPSVADVGGAVFTRPPWNEPLAVARTVASRLLADSS
                     RPGFTLAAAFAGEELIGFGYGHRCSSLAALLHQPTGADFILRELCVTPGHRGWGVGGAL
                     HDAVINSPAPGPRWLITNARAKAAIGLYRQRGWQTVALHPDGAQRLIMRMGPR"
     misc_feature    4703..4718
                     /note="TFBS match to FuR_variant_1, Ferric Uptake
                     Regulator, confidence: weak, score: 17.56"
                     /tool="antismash"
     misc_feature    complement(4703..4721)
                     /note="TFBS match to FuR_variant_2, Ferric Uptake
                     Regulator, confidence: medium, score: 27.36"
                     /tool="antismash"
     misc_feature    complement(4704..4720)
                     /note="TFBS match to NuR, Nickel-responsive regulator,
                     confidence: weak, score: 25.46"
                     /tool="antismash"
     misc_feature    complement(4771..4789)
                     /note="TFBS match to FuR_variant_2, Ferric Uptake
                     Regulator, confidence: medium, score: 28.06"
                     /tool="antismash"
     misc_feature    complement(4772..4788)
                     /note="TFBS match to NuR, Nickel-responsive regulator,
                     confidence: weak, score: 23.52"
                     /tool="antismash"
     gene            5001..6914
                     /locus_tag="AW270_RS12630"
     CDS             5001..6914
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: YcaO"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF014661.4"
                     /locus_tag="AW270_RS12630"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="YcaO-like family protein"
                     /protein_id="WP_157521905.1"
                     /sec_met_domain="YcaO (E-value: 2.3e-47, bitscore: 156.7,
                     seeds: 127, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MLRLTPGHHLHPGPDGVWRHHAPDGRVSLVRAPDEVMRALAREEL
                     PDDEELLRAFEERGLLVAAAQDDPSGIVHLVGDGPIADQVEKLLNPQVKVVRGPLGGAP
                     DMVVACAGWLPDAEWLALDRWCLERGILWHRCHAEGLRFFAGPCGVPYADVRARRLAAA
                     GLPGELVAHWAYLDSGETPPVPWPSAGGVAVVAGLIVADVLAVLSGRTVPACQYEFDPA
                     NNEMIHHPVLSLAEPSGTSLVDPRLGVITKVVREPSAVPSCVIYRAFVADTRTFAPWPA
                     DRVAGGAALEDPARARAAAIGEAVERYCGNAPPQETVRAAYVDLPGAVDPLEFALYSPD
                     QYAAKGFPFVPMTRELVQDWSLGRDLGTGDSVYVPSALVLLNRRREPYTNFQMSAGIAA
                     GKGRAAAEKSALNELYERDALTTWWMRGGPAGEVRLDGPDELVGAVAAAKATGLSVTLL
                     RIPSDYGRVVVGAFVEDAFQRIVGFGAACRATAGEAAAKAFTEACVSYTNSIGLLDPAT
                     YETADHPYRPFRKDCAYRDDFRADWHDLTDLELNLQLYLDPRMQGENLDRLRHPMQITL
                     PEPLPEPRAISVDLTTPDILGTGLRVVRVLVPGLYGNAPAAFPLLGGTRLRPVVLDPLP
                     LA"
     misc_feature    5004..5006
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            6925..7929
                     /locus_tag="AW270_RS12635"
     CDS             6925..7929
                     /GO_function="GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1083: oxidoreductase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /locus_tag="AW270_RS12635"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="WP_061294150.1"
                     /transl_table=11
                     /translation="MRVGLALGYHGEPISELLPLVEHADRIGVDSVWSVEEYGADGVTI
                     LAYLAAKTENIKLGTGILQLAGRTPANTAMTALTLDILSEGRLLLGLGVSVPWIVEGWH
                     GSEFGKPVKRMREYVEILRKAVACDERVSFEGEYYRLPFSDRSRPVKALLDPVRTVIPT
                     YVAGIGPGMTRLAGEVADGWLPGFYSPEREKVMTAPLDEGLGRSGRSAIDITVITHGAI
                     TNDVAKARDELRPLYAAYIGPDIPGVRNTYYELACGLGFEKEAHVIRKHHREKRRAEAV
                     AAVPDQLLDEVALLGPAERIRDRVAAWKESRVTTLALITRDKALLDVVCEAAA"
     misc_feature    7786..7788
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            7926..8915
                     /locus_tag="AW270_RS12640"
     CDS             7926..8915
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1033: iron
                     compound ABC transporter, periplasmic"
                     /gene_kind="transport"
                     /inference="COORDINATES: protein motif:HMM:NF013648.4"
                     /locus_tag="AW270_RS12640"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="WP_169807304.1"
                     /transl_table=11
                     /translation="MRRLLVLALLLTACGQTTPAAAPVEQKAITVQNCGRTLTFDQPPA
                     KVVTGYQPSLETLLALGLQDKIVGRTAFEENGPDGFLPGQKAFYDAIPQISETIMLPQK
                     EVMLAQGADFVIDNGQSSFDPAGGYATVDELQTAGAPVFVLGNWCSPEETLKSTVEQTF
                     IDLRNLGIVFGVQDRAEKLIADLQAQLADVEKRVAGRPKVKVLATDGGTGPVTAYGGAG
                     LMNQMIAKAGGENVLAATQADYSEFSVEQVAAANPEALLVIDYVVYDGKAAPTADKKAA
                     DVLGVAKNSPAATEKRVLPIPAAAAHPGYRNILAIVEMAKFLHPDAFV"
     gene            8959..10023
                     /locus_tag="AW270_RS12645"
     CDS             8959..10023
                     /GO_function="GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_function="GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1011: transport
                     system permease protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018657399.1"
                     /locus_tag="AW270_RS12645"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="iron ABC transporter permease"
                     /protein_id="WP_061294154.1"
                     /transl_table=11
                     /translation="MTVAQKAASQRVSVFRRRSTFGLVLGLLAAAVVFSTWLAVSIGAV
                     QVGLAECWGIVANRLFPGSVTPFWTPVQEQIVWDFRLPRALLALLVGAGLSVVGAVLQA
                     LVRNPLADPFLLGISSGASLGAVAVFVLGASALGGLSLSAAAFGGALLALVLVYVLAQR
                     GGRLIPSRLVLAGVALHFLFQAIYSYILQQAQSGRAAQQVLFWLLGSLGGARWEMLPIP
                     AIVLGAGVAYLLVQHRPLNALAAGEDTATSLGVNVGAFRLQMFVLTSLPVGVMVATSGA
                     IAFVGLMVPHAARLLVGADHRRVLPVSALLGAAFLQLVDIAARTVDAPQELALTIVTAV
                     FGAPFFLWLLRRRA"
     misc_feature    8973..8982
                     /note="TFBS match to AbrC3, Antibiotic production
                     activator, confidence: strong, score: 18.07"
                     /tool="antismash"
     gene            10020..10760
                     /locus_tag="AW270_RS12650"
     CDS             10020..10760
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /GO_function="GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_function="GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020546140.1"
                     /locus_tag="AW270_RS12650"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="WP_061294157.1"
                     /transl_table=11
                     /translation="MRLVLDGVAVTIDGTPIVREADLIVEPGSFAALVGPNGCGKSTLL
                     RTIYRALRPAAGLISLNDEDLRKLKHRETARRIAVVAQEYPAELDFTVEDVVLMGRTPH
                     KRPLSRTTGEDLQRCAEALERVSATGLARRSFVTLSGGEKQRVLIARALAQESSLLLLD
                     EPTNHLDIRHQLDVLHLVRDLGLTTLAALHDLNHALEFADRIYVMDTGRVVADELTPEV
                     IARVFGVRAVSRTQYVFERLKEET"
ORIGIN
        1 agccgaacct ttcgccgatc acctcgatca cgggccggtg catgctgcgc gactcgccaa
       61 ggtcgccctg caccaggttg ccaagccagg tgaagtactg ctcggcgacc ggcttgtcca
      121 ggccccagtt ctcgcggatc tgggccgtga tctcggggct cgtcgtgagc gcgcggaccc
      181 cgagatactg cttgaccggg tcgaacggtg acgccttggc cagcgcgaac atgcccaagg
      241 tgacggcgaa cagcagcggc acggtgaacg ccagccgcca gagcgccatc cgggcgagcg
      301 cccgggtcat gcgaccggct tccactcggc gatgttgcgg aacaggccac cggtctcgtg
      361 ctcgtgcggc tcgacggagg gggcgatccc ggtccactgg ccgcgcacca cgtagacgtg
      421 cttgaggtag acgaggtagg cccaggcggc gtcgtcgctg atgcgctgct ggaaggcgtc
      481 gtaggtctgc ttgcggacgg cggggtcggc ctcctcgcgg cccttgtcga gcagcgcatc
      541 gacctcgggg accttgtaga agcccgggtt gaagaaaccc tgaccggcga gggtcgagtg
      601 gaacagctca tagttggtgt agtcggggtc gtagggggcg ccgtagccca tgaccaaggc
      661 gtccttgctc atgcgcggct cgatcgcgtc ccagtccaga ccggcgagct caatgtcgat
      721 gccgaccttc ttggcgtcgg aggccacggc caggccgagg tcctttcgca gcgcgtcgcc
      781 cgccgggtac atcagggtga agctcgcccg ggtgccgtcc ttgacccgga cgccgtcgtc
      841 accgaccttc cagccggcct cgtcgagaac cttggtggcc tgcgcctggt caggcgcggg
      901 agacccggcg atggccgcgt tgtaccaggt ggtgccgggc gcgatcgggc cataggcggg
      961 ttcgcccgca ccggcgagga tggtgtcgac catcgcctgg cggtcgaggg ccaggttgac
     1021 cgccttgcgg atcgccaggt caccggtgac cggctggccg aaggggaaca tgacgccccg
     1081 atagtcggcg ctgtcgacct tgtggaccgt caggccctgc tggccgtcga agcgcgcggc
     1141 ggccttgggc ggcagcacgg tggcgtcaaa ttccccggcg gccatccggg tggcgcgtac
     1201 gttgtcgtca ccggcgtagg cgaccaccag gttcttgatc gcgggtgcgc cgccccagta
     1261 cgtcgggttg gccttcacga cgagcttgtc gccgggcgtc caggacacga agctgtaggg
     1321 cccggtgccg accggcttgg tgttgaacgg cgcgctctcc aggctctgcc cgtcgagcag
     1381 cttcttcggc acgatgccca gcgtggcccg ctgcaggatc gcggcgtacg ggtacttgag
     1441 gtggaacacc acggtcgagg cgtcgggcgc gtccaccgac tcgatcgccg cgaagtcgcc
     1501 gcggatgctg gagttgttct tctcgtccat gacggcctgg taggtgtaag ccacgtccgc
     1561 gctggtcagc ggggttccgt cgtggaactt gacgtccttg cgcaatgtga aggtgaccgt
     1621 ctcaccgtcg gtcgcgggca tcgcgtccgc cagcgcgggc ttgagcgaga gatcggcgtt
     1681 ccggctgagc agcccgtcga acatgagccc accgccgtcg actgcgtaac ccagaacggg
     1741 gttcagggtg gcgggctcgg aggggatccc gaggacgaac gtgtcgtccg ccgaaccact
     1801 cgacgaggtc tggggaaccg tcggcgcgga gcaagcaccg agggccagcg aggcaattag
     1861 gacgagcgga gcgagacggg cgaacctgac acgaaagggc ttcacgggag tgaaggtaaa
     1921 gcgatcatct tgttattgca agtctgtcgc aagtacatca ggtaacaaac aacgcccagc
     1981 ctggggtggc gcccggacgg tgaaggcggt caccccagcg agatgagatc cggagccggc
     2041 aagcagacaa cgcagccttg cctgaccaca ttcaggcggc ggaacactgg tggcagcggc
     2101 cgagaagcag ctgacctgcc tggttcaggg tgaaccgccg tccggtcgcc aggtcggcgg
     2161 cggtgcggtc caggtcgccg gcggggatct ccagcaacgt gccgcagggt tggcagatga
     2221 ggtgatggtg cggggttatg gccaggccat agagcaactc ccccgcccac tcgatgcagt
     2281 gcagcacgcc tgattcggtc atcgccgtga cgttccggta gacgctcgac gcgttgatgg
     2341 tcggccgtac ggcagccgcc agctgggcca gcgtcgacac gctgtggtgt gtgttcgtcc
     2401 gttccagaat cgtcaggatg gccatccgcg ccccggtccg gcgcagtcct cgatcggcca
     2461 gcatctggtc ggtctcgatg cggtccacct ccctcagttg cgatagatat gctattgcga
     2521 gctctttgca actggaggtc gagatggacg gcgacgtcca cgggcgcatc ggtgaactcg
     2581 cctgcgacga ggtgaccggc gaggtgcagt gccacctgtg cgggcggtcc ttccgcgccc
     2641 tgggcagcca catccgggtc cacgatctga ctgccgacgc ctaccgggag gctttcggcc
     2701 tgttcgcgac caaggcactc acctcccaag agctgtcgga gacccgccgt gaccgccagc
     2761 agcggctgta ccggcgctct ccggccaccc gttccaacct cggaacgggc cagaaactgg
     2821 cccgatctgg agagctcaac actctgacca ggcgagacag tccacaacgt cgcacctccc
     2881 agttgaggga actgaaagac gggcgcgcca cccgctcacg caccacgcaa gagcggctcc
     2941 gcaccgcgct cgccgacgcc ggtttccccg acagtgccgc cgggctgcgc acgctgtacg
     3001 tcgacaggca gaccagcatg gagaacctcg cggccctgct cggcgtcagc cgcgtcaccc
     3061 tgcgcaacgc cctgctggcc gccgacatcc cgttgcgcgc catcggagtg aacaccgaca
     3121 cgggacgacg ctcgcggatc gcctccaaca tcgcgcgcgc ggccgcactt gtcggaacca
     3181 ccgacctgca ccaatggcta cacgagcgcc gctcgcaagg ggcgagtctc cagcagctcg
     3241 cgaccgagct gaaccgcagt gtgccgtggg tgaaggaccg gctctcggag cggagtgatc
     3301 atgagggccg cgcgcggtcg agcagaccgg ccgtacctcc gggctgatgg gtgccggccg
     3361 cctccagcga agacgacgag aaggtcgaac gccacgctgg tctgagaggg gacgccgcgt
     3421 ccgggcggga aagcagccac tcgatccggc ccggacgcgg cgagttctag gcgcgcgccg
     3481 cgtcgaagcg cgcgatgacg tcgttccagt tgatgagcga ccagagcttc tcgacgtagt
     3541 caggccggac gttcttgtac tgcaggtagt aggcgtgctc ccaggcgtcg aacaccagca
     3601 gcggggtcga gctcatgccg acgttgccgt ggtggtcgta gacctgctcg acgatcagac
     3661 gacggcccag gggctcccac gccagcacgc cccagccgga gccctgcacg gtcgccgtgg
     3721 cggtcgtgag ctgcttctgg aaagcctcga aggagccgaa atgttcgacg atcgagtcgc
     3781 cgagttcgcc gtcggggcgg tcgccgccgt ccggggagag gttctgccag aagatcgtgt
     3841 gcaggacgtg gcccgacagg ttgaacgcgt aggtcttctc caggccgacc aggccgctga
     3901 agtcgccctt gtcacggact tcggcgagcc ggtcgagcgt gtcgttggcg cccttcacgt
     3961 aggcggcgtg gtgcttgctg tggtgcagct ccaggatctc gcccgtgatc gccggctcca
     4021 gcgccgcgta gtcgtagggc atatccggaa gggtgtactg gctcacttgc ccatcctcct
     4081 tattgcacat tgatcgcaac agcaatgagt acacaataag ggacgtgatc tcggcccctg
     4141 tgatcagcgt ggtcccatcc gcatgatcag ccgttgggcg ccatctggat ggagggcgac
     4201 ggtctgccag ccgcgctgcc ggtagaggcc gatcgcggcc ttcgcgcggg cgttggtgat
     4261 cagccagcgc gggcccgggg cgggcgagtt gatcacagcg tcatgcaggg cgccgcccac
     4321 accccagcca cggtggccgg gggtcacaca cagttcccgc aggatgaagt cggcgccggt
     4381 cggctgatgg agcagggcgg ccagggacga acagcggtgg ccgtacccga acccgatcag
     4441 ctcctccccg gcgaaggcgg cggcaagggt gaagccgggc cgggagctgt cggccaacag
     4501 gcgggaggcc acggtgcggg caaccgcgag aggctcgttc cagggcggcc gggtgaacac
     4561 ggccccgccc acgtcggcga cggatggcag cagcgaccac aggtccgacg acttcacgta
     4621 ggcgatgact gggcgatgag gctctgggtg accagcgacc acgtcttctc caggtgaccg
     4681 ggcgggaggc ttgcgcctca ctaatgcgag tctttcgcaa taacataatt caggcaacaa
     4741 ggggttgacc cacccgaggt gccgacttag gttgcgaatc gttcgcactt gcgaatgaac
     4801 ggcaagcaat ctcgaaggag gacggcccat ggcacatccc acgttcgaag agctggccga
     4861 gtcgagcgtg tgcatccgct cgcacacgcc cgccgtggac gaggcgttca cctggcacga
     4921 ggacgacgcc gagtagtccc ccgcgggagg ccgagctcgc aagcggcctc ccgcaccccc
     4981 ggccacggag gattcgaacg ttgttacgtc tcacccctgg gcaccacctg catccaggcc
     5041 ccgacggggt gtggcggcac cacgctcccg acggccgggt gtccctcgta cgcgccccgg
     5101 acgaggtgat gcgcgctctg gcccgagagg aactgcccga cgacgaggag ttgctccgcg
     5161 ccttcgagga gcgcgggctg ctggtcgcgg cggctcagga cgacccgtcc gggatcgtgc
     5221 atctcgtcgg cgacgggccc atcgcggatc aggtggagaa gctcctcaat ccccaggtca
     5281 aggtggtgcg cgggcctctc ggcggcgctc ccgacatggt ggtggcctgc gcgggctggt
     5341 tgccggacgc cgagtggctg gcgctcgacc ggtggtgcct ggagcggggc atcctgtggc
     5401 atcgctgcca cgcggagggg ctgcggttct tcgcggggcc gtgcggggtg ccctacgccg
     5461 acgtccgggc gcgacggctg gccgccgccg ggctacccgg ggagctcgtc gctcactggg
     5521 cctatctgga ttccggggag acgccgccgg tgccgtggcc ctcggcgggt ggggtcgcgg
     5581 tcgtggccgg gctgatcgtc gccgacgtgc tggccgtctt gtcggggcgg accgtcccgg
     5641 cctgccagta cgagttcgac ccagccaaca acgagatgat ccaccatccg gtgctgtctt
     5701 tggccgaacc ctccgggacc tctctcgtcg accctcgcct cggggtgatc acgaaggtgg
     5761 tgcgtgagcc gagtgccgtg ccgtcgtgtg tgatctaccg ggcgttcgtg gccgacaccc
     5821 ggaccttcgc gccctggccg gccgatcggg tcgccggagg cgcggcgctc gaggatccgg
     5881 cgcgggctcg ggccgccgcg atcggggagg cggtcgagcg gtactgcggg aacgcgccgc
     5941 cgcaggagac cgtccgggcg gcttatgtcg atctgcccgg tgcggtcgat ccgctggagt
     6001 tcgcgctcta ctcccccgat cagtacgcgg ccaaaggatt tcccttcgtt ccgatgaccc
     6061 gcgaactggt gcaggactgg tcgctcggcc gggatctcgg cactggggat tctgtgtacg
     6121 tgcccagtgc gctcgtgctg ctcaaccgca ggcgggagcc gtacacgaac tttcagatga
     6181 gcgcggggat cgcggcggga aagggacgcg cggcggccga gaagtcggcg ctgaacgagc
     6241 tctacgagcg ggacgcgctg accacctggt ggatgcgcgg cggcccggcc ggagaggtca
     6301 ggctcgacgg acccgacgag ctggtcggtg cggtcgccgc cgcgaaggcc acggggctct
     6361 cggtcacgct gctccggatt ccctccgact acgggcgcgt cgtggtcggc gcgttcgtcg
     6421 aggacgcctt ccagcgcatc gtcgggttcg gcgcagcgtg ccgggcgacg gcgggtgagg
     6481 cggcggcgaa ggcgttcacc gaggcctgcg tgtcctacac caacagcatc gggctgctcg
     6541 acccggcgac ctacgagacc gccgatcacc cctaccggcc ctttcggaag gactgcgcct
     6601 accgcgacga cttccgcgcc gactggcacg acctcacaga cctggagctg aacctgcagc
     6661 tctacctcga cccacgcatg cagggcgaga acctcgaccg gctccggcat ccgatgcaga
     6721 tcaccttgcc tgaaccgctg cccgaacccc gtgcgatctc cgtcgacctg accacgccgg
     6781 acatcctcgg caccggtctg cgggtggtgc gggtgctcgt tcctggcctg tacggcaacg
     6841 ccccggcagc ctttcctctg ctcggcggca cccggctgag gccggtcgtc ctcgatcctc
     6901 tccctctcgc ctaggagctg tgacatgcgt gtgggcctgg ccctcggcta ccacggagaa
     6961 ccgatctcgg agctgctgcc cctggtcgag cacgccgacc gcatcggcgt cgactcggtg
     7021 tggagcgtcg aggagtacgg cgcggacggg gtgacgatcc tcgcctacct ggccgcgaag
     7081 accgagaaca tcaagctcgg caccggcatc ctccagctcg ccgggcgcac cccggccaac
     7141 accgcgatga ccgcgctgac gctcgacatc ctgtcggagg gccgcctgct gctcgggctg
     7201 ggtgtgtcgg tgccgtggat cgtcgagggc tggcacggtt cggagttcgg caagccggtc
     7261 aagcggatgc gcgagtacgt cgagatcctg cgcaaggccg tcgcctgcga cgagcgggtg
     7321 tccttcgagg gcgagtacta ccgcctgccc ttctccgaca ggtcccggcc ggtcaaggcg
     7381 ctgctcgacc ctgtccggac cgtcatcccc acctacgtcg ccgggatcgg gcccggcatg
     7441 acccggctgg ccggggaggt cgccgacggg tggctgcccg gtttctactc cccggagcgg
     7501 gagaaggtca tgaccgctcc gctggacgag ggtctcgggc ggtcgggccg atccgcgatc
     7561 gacatcaccg tcatcaccca cggtgccatc acgaatgacg tggccaaggc gcgggacgag
     7621 ctgcgccctc tctatgccgc ctacatcggc ccggacatcc cgggcgtccg caacacgtac
     7681 tacgagctcg cctgcgggct cgggttcgag aaagaagcac atgtgatccg caaacaccac
     7741 cgcgagaaga ggcgggccga ggccgtcgcc gccgtcccgg accagttact cgacgaggtc
     7801 gccctgctcg ggccggccga gcgcatccgt gaccgggtgg cggcctggaa ggagtcgcgg
     7861 gtgaccacgc tcgcgctcat cacccgcgac aaggccctgc tcgacgtcgt gtgcgaggcc
     7921 gcggcgtgag acggctcctc gttctggccc tcctgctgac cgcgtgcggg cagaccaccc
     7981 cggccgccgc accggtcgag cagaaggcca tcacggtgca gaactgcgga cggacgctga
     8041 ccttcgacca gccaccagcc aaggtcgtta ccggctatca gccctcgctg gaaaccctgc
     8101 tcgccctcgg cctccaggac aagatcgtcg ggcggaccgc gttcgaggag aacggccctg
     8161 acggcttcct gcctggccag aaggccttct atgacgccat tccgcagatc tccgagacga
     8221 tcatgctgcc gcagaaggag gtcatgctcg cccagggcgc cgacttcgtc atcgacaacg
     8281 ggcagagcag cttcgacccc gccgggggct acgcgaccgt cgacgagctc cagaccgccg
     8341 gggcgccggt gttcgtgctg gggaactggt gcagccccga ggagacgctg aagtcgaccg
     8401 tcgagcagac cttcatcgac ctgcggaatc tcgggatcgt cttcggcgtc caggaccggg
     8461 ccgagaagct gatcgccgac ctccaggccc agctcgccga tgtcgagaag cgggtggcgg
     8521 ggcggccgaa ggtgaaggtc ctggccaccg acggcggcac cgggcctgtc accgcctacg
     8581 gcggcgcggg cctgatgaac cagatgatcg cgaaggccgg cggcgagaac gtcctggccg
     8641 ccacccaggc cgactattcc gagttcagcg tcgagcaggt cgccgccgcg aacccggagg
     8701 cactgttggt gatcgactat gtggtgtacg acggcaaggc ggcccccacg gcggacaaga
     8761 aagccgccga cgtgctcggg gtggcgaaga acagcccggc agcgaccgag aagcgcgtgt
     8821 tgccgatccc cgcagcggcg gcgcatccgg ggtaccggaa catcctggcc atcgtcgaga
     8881 tggccaagtt cctgcaccca gacgctttcg tctgatgaaa ccgatcatcg acaggccgac
     8941 aaacgacgag gccgccttat gacggtggcg cagaaggcgg cgagccagcg cgtctccgtc
     9001 ttccgacgca ggtcgacgtt cggtctggtc ctcggcctgc tggccgcagc cgtcgtgttc
     9061 tccacctggc tggccgtcag tatcggcgcg gttcaggtcg ggctggccga gtgctggggc
     9121 atcgtcgcca accggctgtt ccccgggtcg gtcaccccgt tctggacccc ggtccaggag
     9181 cagatcgtct gggacttccg gctcccccgc gcgctgctgg cactcctggt cggggccggg
     9241 ctgtcggtgg tcggcgcggt cctccaggcg ctcgtgcgca acccgctcgc cgacccgttc
     9301 ctgctcggca tctcctcggg cgcctcgctc ggcgcggtgg cggtgttcgt gctcggggcg
     9361 tccgccctag gcgggctgtc gttgtcggcc gccgcgttcg gcggggcgct gctggcgctt
     9421 gttctcgtgt acgtgctggc gcaacgcggc ggcaggctca tcccgtcgcg gctggtcctg
     9481 gcgggggtcg cgctgcactt cctgttccag gcgatctatt cgtacatcct ccagcaggcc
     9541 cagagcggcc gggccgccca gcaggtgctg ttctggctgc tcggatcgct cggcggcgcg
     9601 cggtgggaga tgctcccgat cccggcgatc gtgctgggtg ccggcgtcgc ctacctcctg
     9661 gtccagcacc ggcccctcaa cgccctggcc gccggggagg acaccgcgac ctcgctcggc
     9721 gtgaatgtgg gcgccttccg gctccagatg ttcgtgctga cctcgctgcc cgtcggcgta
     9781 atggtcgcca ccagcggggc catcgccttc gtcggcctga tggtgccgca cgccgcccgg
     9841 ctgctcgtcg gggccgacca ccgccgggtg ttgccggtgt cggccctgct gggagcggcg
     9901 ttcctccagc tcgtggacat cgcggctcgg actgtggacg cgccgcagga gctcgcgctg
     9961 accatcgtca ccgccgtctt cggcgcgccg ttctttctgt ggctgctgcg gaggcgggca
    10021 tgaggctggt cctggacgga gtggccgtca ccatcgacgg cacgccgatc gtgcgcgagg
    10081 ccgacctcat cgtcgagccg gggagtttcg cggccttggt cggccccaac ggctgcggga
    10141 agtcgacgct cctgcggacg atctaccggg cactacgccc ggcggccgga ctcatctcgc
    10201 tcaacgacga ggacctccgc aagctcaagc accgcgagac cgcccgccgg atcgcggtcg
    10261 tcgcccagga gtaccccgcc gaactcgact tcaccgtcga ggacgtcgtg ctcatgggcc
    10321 gcacccccca caaacgcccg ctctcccgca ccaccggcga ggacctgcaa cgctgcgccg
    10381 aggcgctcga acgggtctcg gcgaccggcc tggcgcgcag gtccttcgtc accctgtccg
    10441 gcggcgagaa acagcgggtc ctgatcgccc gcgccctcgc tcaggagagc agcctgctgc
    10501 tgctcgacga gccgaccaac catctcgaca tccgccatca gctcgacgtc ctgcacctgg
    10561 tccgcgacct gggcctgacc acgctcgccg cgctgcacga cctcaaccac gccctggagt
    10621 tcgccgaccg gatctacgtc atggacaccg gccgcgtcgt cgccgacgag ctcacccccg
    10681 aggtcatcgc gcgggtcttc ggcgtgcgcg cggtgtctcg cacccagtac gtcttcgaac
    10741 ggctcaagga ggagacatga tcgcgcggct gctgtccccg gcggtgcgcc tcaggctggc
    10801 cgcgctggtg gcgatcctgc tgaccatcac ggccacctac gtgttccagg gcgtgctgat
    10861 cgcccactcc ctggcggcga tcttcgccgg agacggcttc ctgtggccgg tcatcgggat
    10921 cgctctcctt caggtggtgc gaagctgcgc actagccgtg cgggagacgg cttcgctacg
    10981 gacatccggg ctggtcaagg ccgagctccg ggaacgcctg gtggccaagc tgctggtcct
    11041 cgggcctggg tgggctcagc gaggtcgtac cggagcaaca cagacctcgc tcgtcgatgg
    11101 catcgagacc gccgacgcct tcgtgggccg gttcctgccg caggtgatcg cggcgatcat
    11161 cggcgcggtc gcggtgacgg cgtacctgtt caccctcgac atcgtcgtcg ggctggtggt
    11221 gctgggctgc tcgatcctgc tgccggtgct gccgctggtg tccgagacgg tgctgcgcag
    11281 ccgcaacaag gcgtggttca ccgcctacaa ggccctgtac tccgagaacc tcgacgccct
    11341 ccagggcatg gtgaccctga aggcgttcaa cgccagccgc cgccggggaa ccgaactcgc
    11401 caccctggcc gagaagttct gcaccgactc catccggctc atggccgtgg tcgtcctgta
    11461 cgtcggcggt gtcgccttcg tcatcgcgac cggcaccgcc gtggccatcg gggtaggcgc
    11521 ctaccggctg acggccggag agatcacggt caccgaactg ctgctcatcc tgatgctgac
    11581 cgccgagtgc ttccgcccgc tgcgcgacct ggaggcggcc taccactcca gctacaacgc
    11641 catcccgtcg ctgcgcggag tgctggaggt gctcgacgaa cccgtacagg aagcgggaac
    11701 cgactccgtc accggccccg cgcgggtgac cttcaccgac gtcaccttca cctatcgaga
    11761 ccgcaccagg cccgccctaa gcgacttctc acttgacgtc gccccgggcg aacgggtggc
    11821 gctggtcggc agatcggggg ccggcaagac cacggtcgcc gcgctgctgc tgcgcttctt
    11881 cgacaccagc caggggcgca tctccgtgaa cggc
//
