LOCUS       NZ_BBXG01000004        10456 bp    DNA     linear   CON 08-AUG-2024
DEFINITION  Herbidospora cretacea strain NBRC 15474, whole genome shotgun
            sequence.
ACCESSION   NZ_BBXG01000004
VERSION     NZ_BBXG01000004.1
KEYWORDS    WGS; HIGH_QUALITY_DRAFT; RefSeq.
SOURCE      Herbidospora cretacea
  ORGANISM  Herbidospora cretacea
            Bacteria; Actinomycetota; Actinomycetes; Streptosporangiales;
            Streptosporangiaceae; Herbidospora.
REFERENCE   1
  AUTHORS   Komaki,H., Ichikawa,N., Oguchi,A., Hamada,M., Tamura,T. and
            Fujita,N.
  TITLE     Genome-based analysis of non-ribosomal peptide synthetase and type-I
            polyketide synthase gene clusters in all type strains of the genus
            Herbidospora
  JOURNAL   BMC Res Notes 8, 548 (2015)
   PUBMED   26452464
  REMARK    DOI:10.1186/s13104-015-1526-9 Publication Status: Online-Only
REFERENCE   2  (bases 1 to 650410)
  AUTHORS   Hosoyama,A., Oguchi,A., Ichikawa,N., Kimura,A., Ohshita,R. and
            Fujita,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAY-2015) Contact:Director-General Department Of
            Biotechnology National Institute of Technology and Evaluation,
            Biological Resource Center (NBRC); 2-49-10 Nishihara, Shibuya-ku,
            Tokyo 151-0066, Japan URL :http://www.nite.go.jp/nbrc/index.html
COMMENT     ##Genome-Assembly-Data-START##
            Finishing Goal                    :: Noncontiguous Finished
            Current Finishing Status          :: High-Quality Draft
            Assembly Method                   :: newbler v. 2.6
            Genome Coverage                   :: 71x
            Sequencing Technology             :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_001570605.1-RS_2024_08_08
            Annotation Date                   :: 08/08/2024 02:39:29
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,566
            CDSs (total)                      :: 7,487
            Genes (coding)                    :: 7,430
            CDSs (with protein)               :: 7,430
            Genes (RNA)                       :: 79
            rRNAs                             :: 4, 5 (5S, 16S)
            partial rRNAs                     :: 4, 5 (5S, 16S)
            tRNAs                             :: 67
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 10 of 57
            Pseudo Genes (incomplete)         :: 49 of 57
            Pseudo Genes (internal stop)      :: 3 of 57
            Pseudo Genes (multiple problems)  :: 4 of 57
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 12:10:14
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 203980
            Orig. end                         :: 214436
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            BBXG01000004.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10456
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="3"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10456
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10456
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:5456](-)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5456)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     misc_feature    25..39
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: weak, score: 18.46"
                     /tool="antismash"
     gene            complement(2074..2781)
                     /locus_tag="AW270_RS09075"
     CDS             complement(2074..2781)
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /GO_function="GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_function="GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012886892.1"
                     /locus_tag="AW270_RS09075"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="WP_218009132.1"
                     /transl_table=11
                     /translation="MKVRLEAVRRTYGTVTALDDVTATFDAGTLTAIMGPSGSGKSTLL
                     HCAAGLDRPTGGTVVIDGTDLGSLSEHGLTVLRRERIGFVFQAFNLVSALTAEQNVALP
                     LRLAGRPAAPELVRGALARVGLADRAHHRPNGLSGGEQQRVAIARALITRPAVVFADEP
                     TGQLDTQTSRQVLGMLRALVDDHGQTMIMVTHDPVTASCADRVIFLEDGRVVDELTAPL
                     TAEAVAARMAAAC"
     gene            2892..4094
                     /locus_tag="AW270_RS09080"
     CDS             2892..4094
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1048: sensor
                     histidine kinase"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017586004.1"
                     /locus_tag="AW270_RS09080"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="sensor histidine kinase"
                     /protein_id="WP_157521780.1"
                     /transl_table=11
                     /translation="MLIQTAMTAMTRRPLAFLSSSWPWRALAYLLASAVYAVTACLLIY
                     TAYLYGTGTAAAAALLVLVLLALPAAAFERWRLRLVDRVPVDDPHVKPPERGVRAWVFT
                     RAREQATWREFGFTVVALSVLCWMDLGLVSSAFWLPALLMSGPFQPSLHWADGLPLALG
                     GVLLVPLSAYMLVAWAGTRAMITRAVIAPGDAEVIRSRARLVDAFEAERRRIERDLHDG
                     AQQRLVSLSMKLGLARLDLPPGSPAETQVADAHEEAKRALAELRELIRGIHPQVLTDRG
                     LGAAVEDVAGRSPIPVDVDVSLPGRLPPPIEAAAYYVVSEAMTNIAKHSGARHGLVYGR
                     LRDGALVVEVRDDGVGGADPAGGSGLAGLADRVAVTDGVMTLSSPPGGPTSLRVEIPCL
                     I"
     misc_feature    4065..4067
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            4366..5019
                     /locus_tag="AW270_RS09085"
     CDS             4366..5019
                     /GO_process="GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1016: LuxR family
                     DNA-binding response regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012854964.1"
                     /locus_tag="AW270_RS09085"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="response regulator transcription factor"
                     /protein_id="WP_061292769.1"
                     /transl_table=11
                     /translation="MPRLRIALAEDGVLLREGLVGLLDRFGHDVVAAVGDGEALIAAVA
                     EHEPDIIVADARMPPGFTDEGLRAGLTLRASRPRLAVLVLSQYVEQTYAAELLDSGGGV
                     GIGYLLKDRIGEVREFVDAVERVAAGHTVVDPEVVRQLLNRRREPLSRLTPREREVLGL
                     VAEGRSNAAIARALVVTEAAVAKHIASILAKLDLPPAEDDHRRVLAVLAYLRGR"
     gene            complement(5001..5456)
                     /locus_tag="AW270_RS42095"
     CDS             complement(5001..5456)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: YcaO"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF014661.4"
                     /locus_tag="AW270_RS42095"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="YcaO-like family protein"
                     /protein_id="WP_369408986.1"
                     /sec_met_domain="YcaO (E-value: 1.7e-07, bitscore: 26.4,
                     seeds: 127, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MHFGRPQSLSVSRSHSDPADAAPARQRPRRTPTLMPLRTQPHASP
                     AATAPGLVQTRAYHRRPVAMARAPVEERGMEMLALDQTVPDPGLPVVEVVVPGSHHLWW
                     QLTAAGYTTCPPSSAWCPNRPRPDSNPRLNPTAGTIQAHLPRHRPRR"
     misc_feature    5740..5755
                     /note="TFBS match to RutR, Regulator of the pyrimidine and
                     purine metabolism, confidence: weak, score: 19.41"
                     /tool="antismash"
     misc_feature    5861..5876
                     /note="TFBS match to RutR, Regulator of the pyrimidine and
                     purine metabolism, confidence: weak, score: 19.41"
                     /tool="antismash"
     gene            complement(6007..6798)
                     /locus_tag="AW270_RS09090"
     CDS             complement(6007..6798)
                     /EC_number="3.5.3.-"
                     /GO_function="GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF012703.4"
                     /locus_tag="AW270_RS09090"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="arginase family protein"
                     /protein_id="WP_061292772.1"
                     /transl_table=11
                     /translation="MTTVLCVPQWQGSAHARAPRLTEGAHRASALITADTVVTVPTPGT
                     TPKSGGIRARDTLIEVQRRTFEALEGVDDRIITVGGDCAVDIPSIATAHDRYGDALTVL
                     WIDAHPDVYSPSTLPSGAFHGMVVRALLGDDELAGLTPDRPLKPSQVIIAGERAGADSE
                     HAYLREQGIRRYGVPDLHRAFEGLTGPVYVHVDLDVLDPEVFGSTCYPEPGGVHPDRLA
                     DLIAGLDGVIGAALTEHAPLNQIPHEDNVIRTVSEALNSAF"
     gene            6883..7104
                     /locus_tag="AW270_RS09095"
     CDS             6883..7104
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017587592.1"
                     /locus_tag="AW270_RS09095"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_061292775.1"
                     /transl_table=11
                     /translation="MRKGPRLLGLLLFAMGVSGTVDHLAAQPVMGVLLNAPNRWIFERL
                     EVFEGREVFANLSLAFVGVVLVAFSFRR"
     misc_feature    7113..7126
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 13.91"
                     /tool="antismash"
     gene            7135..7545
                     /locus_tag="AW270_RS09100"
     CDS             7135..7545
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020541023.1"
                     /locus_tag="AW270_RS09100"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_061292777.1"
                     /transl_table=11
                     /translation="MQPYSVEQVANLLGLHVKTVRNYVRDGKLKAVRIGKQYRITREDL
                     EAFTGQPVVPEAVRRQRGVEVSAVVQVDAISRERTDRLTNALIAAANSGGEGKLRVEAI
                     YDEVRASLKIIVIGGAADAAALLTIVDSLTQE"
     gene            7548..7886
                     /locus_tag="AW270_RS09105"
     CDS             7548..7886
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019631322.1"
                     /locus_tag="AW270_RS09105"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="DUF4180 domain-containing protein"
                     /protein_id="WP_061292779.1"
                     /transl_table=11
                     /translation="MHFTLPADGPPIATEADALDVIGATFGQGVTWVVVPAERLSDAFF
                     SLRTGLAGAVLQKFVQYGLRLAVIGDVSRHVAASDAFRDLVRECNRGDQTWFLPTTEEF
                     DARVTARR"
     gene            complement(7870..8166)
                     /locus_tag="AW270_RS09110"
     CDS             complement(7870..8166)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012851057.1"
                     /locus_tag="AW270_RS09110"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="AzlD domain-containing protein"
                     /protein_id="WP_061292781.1"
                     /transl_table=11
                     /translation="MDMPIAAIVALAAGTLAFRLAGPLLRGRVRLPPRLEALLSTAALV
                     LLFALVVTSSSGGWARPAGVVVAGALALRRAPFPVIVIGAAATTALLRLIGVP"
     gene            complement(8244..8888)
                     /locus_tag="AW270_RS09115"
     CDS             complement(8244..8888)
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_component="GO:1902495 - transmembrane transporter
                     complex [Evidence IEA]"
                     /GO_process="GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009079354.1"
                     /locus_tag="AW270_RS09115"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="AzlC family ABC transporter permease"
                     /protein_id="WP_082801420.1"
                     /transl_table=11
                     /translation="MRSISRTALRDIALVCVADAIVGASFGAIAVSGGFDPWVPVVMSL
                     IVFAGGAQFAAIGILMGGGGAVAAVATGLILNARLLPFGFAVADVVGNRLPGVHLITDE
                     TVAFALKGAGPRERHDYFWTSGLALFAAWNASVVAGALAGAAIGDVGALGLDAAFPAVL
                     VALIMPALADRGVRVPVLTGGVIALAASTVLPAGLPVLLSLLGLLARPKEA"
     gene            8957..9514
                     /locus_tag="AW270_RS09120"
     CDS             8957..9514
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="other (smcogs) SMCOG1148: hypothetical
                     protein"
                     /gene_kind="other"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012851055.1"
                     /locus_tag="AW270_RS09120"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_061292784.1"
                     /transl_table=11
                     /translation="MDTSAALITLIAASIRRERDRVGLSLTELAKRAGIAKSTLSQLEA
                     GGGNPSVETLWALAVALGVPISRLIDPPRPHVRVIRAGEGPVTHSERADYAATLLASCP
                     PGARRDLFRIDAEPGEARTSDPHNPGTLEHVIMGLGRALVGPTEGPVELGPGDYITYPG
                     DVPHVFRALEPRTTAVIVMEHV"
     gene            9511..10218
                     /locus_tag="AW270_RS09125"
     CDS             9511..10218
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017622504.1"
                     /locus_tag="AW270_RS09125"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="WP_061292786.1"
                     /transl_table=11
                     /translation="MTELHSDCASCAGLCCVELPFTRSADFAVDKPAGKPCANLTGDFR
                     CRIHDRLRESGYQGCVTFDCFGAGQRVTRARLDVALFPLIRQLHELLWYLEEASRHGHT
                     ADLLKETEALAASPVIDPLQIGELRGRVGARLGEISERVRGRSREMRRDLAGAKLRGQD
                     LRNVSLRGRLLIGADLRGADLRTADLLGADLRGADVRGADLRGALFLTRPQVAAARGDR
                     TTRLDLDRPGHWT"
ORIGIN
        1 cgcggacgcg tccggccgcc gacatcacca tcgtgttcac gatcgccaca cccgcgtagg
       61 cggtggagac cacgacgagg agcagggtcg cgatccacac gagccggtcc tcctcggcgt
      121 cggcctcggc cgagtaggtc gcgacgtcca ccacgcgagc ccccgccccg gggtcggcgg
      181 ccgggccgac gacgtaggcc tcctcggcga ggaccacggg gtcgtgtctc cggagcaggc
      241 cccgggagat cagcacgtcg gcgccggcgt ccttgaccgt gcccgcgacg gtcaggcgag
      301 cggaggtgcc gtcttcgaag gtcaccggca tcgtcgcgcc cggcctcagg ccgcgatcct
      361 cggccagcca gtcggccacc aggatccggt cgtcgtggcg gagggcgtcg agcgggcccg
      421 tggcgtcacc ggcgcgggcc aggagttcgg gggtcgcccc gatcgtgctg agagggtcgc
      481 cgttcggctc gaagagggcg ctgaacaggg acgactcacc accggaggcg tcggtcaggc
      541 cgggcgtccc gtcggggacg acgaccgcgt cggcgcggat cgtggtggcg cggacggccg
      601 cgaacccggc ggcggtcgtc tgcaccatcc cggtgatcag caccgcgcag cccacggtga
      661 ccagcaccgg ggtggccgtg gcggcggtcc tgcggacggc ggtccgggcg ttctcccgga
      721 cgagcaggcc gaccgcaccc cgccctcggg cgaacggcca cgacacgagc cgggccacgg
      781 gcgggaccac gacgggggac agcagcgcca gaccggcgat cacccccatc gcggcgacca
      841 ggcccgacat cacgaacgac ccttcggggt cggagcccag cgccgaggcc acgcccagcg
      901 cgcagagggc cagcccggtg atccaccgca cgcgcggcat cggccggtcg tccacctcgg
      961 cctcgcgcag cgcctccaga gggcgcaccc gcgcggcccg ccgggacgcc gaccacaccg
     1021 cgaccagcgt cacgaccacc ccggccacga acgagccggc gagggggagc ggcgacaggc
     1081 tcaccgtgaa gccgtcgggc tcgaaaccga cgtcgaccag caccgcgccc agcacggggg
     1141 cgagtgacgc gccggccgcc acccccgcgg ccgtggcggc gacgccgacg acgagcgcct
     1201 cgccgtagac catccgccgg atctggcccg gcgtcgcgcc gacggcacgg aggagtccga
     1261 actcccggcg gcgatgcgcc accccgaccg cgaacgtcga ccccacgacc aggatcgaga
     1321 cgaaacccga gagcacggcc atgccggtga ggacctggag gccgatccag cgggtccgct
     1381 cgtcgtcccg cgactccaac gcgctccggg cgtctccggt gaggactgat cgccccacgt
     1441 gggccgaagc ttcccctgcg gccttcgcga ccgccgcgat gtcggctccc ggttcggtga
     1501 ccaggccgat cgcgcgtatc ccggccgaga ggcgcgccgc ctcggcgtcg ctcaggtaga
     1561 cccccggccc gtcgactgtg ccggacacgg tgtaggtcac cggaccgcgc acggtcagca
     1621 cggacacccg tgcccccgga cgaagaccca gggaccggtc gagcgccact tcgccccggc
     1681 cgggtgcggt gcccgccgtc agcgggtagg gcgcgagcat cgccgtcgac caggcgtagc
     1741 ctcggtcgga cgcgatgggg cggcctgaga tgaccggctg ggcgtagaag gtccggtcgg
     1801 ggatcgcccg tctcacgccg gggatcatgg ccaggcggtc gagcagggcg gtgacctgct
     1861 ggggtgacca cggccggtcg ggggcgaagt cgccgtcttg gcgatcggtc tgcggggccg
     1921 agacgaacac gggtgttccg gcgaaccggc cgggcacctc cgggccggac gaggtcaggg
     1981 ccagggtgct catcgagacc agcgtcatgc cgaggagcag cacgacgaag cctccggcga
     2041 aggagtgcca ccgctcccgg acggtcgcga agctcaacat gccgcggcca tccgggcggc
     2101 gacggcctcg gccgtgaggg gggcggtgag ttcgtcgacc acgcggccgt cttcgaggaa
     2161 gatcacccgg tcggcgcagg aggccgtcac cgggtcgtgg gtcaccatga tcatcgtctg
     2221 gccgtggtcg tcgaccaggg cccgcagcat ccccaggacc tggcgtgagg tctgggtgtc
     2281 gagctggccg gtcggctcgt cggcgaagac cacggcgggc ctggtgatca gcgctcgcgc
     2341 gatggccacc cgctgctgct cgccgcccga cagcccgttc ggccggtggt gggcgcggtc
     2401 ggcgagaccg acccgcgcca aggccccgcg gacgagctcg ggggcggccg ggcggccggc
     2461 cagacgcagc ggcagcgcca cgttctgctc ggcggtcagc gccgagacca ggttgaacgc
     2521 ctggaagacg aacccgatcc gctcgcgccg cagcacggtc aggccgtgtt cactcaggga
     2581 gccgaggtcg gtgccgtcga tgacgaccgt cccgccggtc gggcggtcga gcccggccgc
     2641 gcagtggagc agtgtcgact tccccgagcc cgacgggccc atgatcgccg tcagggtgcc
     2701 cgcgtcgaag gtggccgtca cgtcgtcgag ggcggtcact gtgccgtagg tgcggcgtac
     2761 cgcttccaat cgaaccttca tggggtccag cgaactcgct cggccccggc ggcacattgg
     2821 tgctgcggcg agagacgaag gtagtcctgg cactaccgtg ccccgccggg ctgatcggtt
     2881 acggtcgggg cgtgctgatc cagaccgcca tgacggcgat gacgcgccgg ccactggcgt
     2941 tcctgagctc gtcgtggccg tggcgcgcgc tcgcctacct gctggccagc gcggtctacg
     3001 ccgtgaccgc gtgcctgctc atctacacgg cctacctcta cggcacgggc accgcggccg
     3061 ccgccgccct gctcgtcctg gtgctgctgg cgctgccggc ggcggcgttc gagcggtggc
     3121 ggctgcggct ggtcgaccgg gttcccgtcg acgaccccca cgtgaaaccc ccggagaggg
     3181 gagtccgggc ctgggtcttc acccgggcac gggagcaggc gacctggcgc gagttcgggt
     3241 tcacggtcgt cgcgctgtcg gtgctgtgct ggatggacct ggggctggtc tcctccgcct
     3301 tctggctgcc ggcgctgctg atgtcggggc cgttccagcc gagcctgcac tgggccgacg
     3361 gcctgccgct ggcgctgggc ggagtgctgc tggtgccgct ctcggcgtac atgctggtgg
     3421 cctgggcggg cacccgggcc atgatcacgc gggcggtcat cgcgcccggc gacgccgagg
     3481 tcatccgctc gcgggcccgg ctggtcgacg cgttcgaagc cgaacgccgc cgcatcgagc
     3541 gcgacctcca cgacggcgcg cagcagcggc tggtctccct cagcatgaag ctcggcctgg
     3601 cccgtctcga cctgccgccc ggttcgcccg ccgagacgca ggtcgccgac gcccatgagg
     3661 aggccaaacg cgccctggcc gagctgcggg aactcatccg cggcatccac cctcaggtgc
     3721 tcacagaccg agggctcggc gcggccgtgg aagacgtggc ggggcgatcg cccatcccgg
     3781 tcgacgtcga cgtctcactc cccggccggc tgcccccgcc gatcgaggcc gccgcctact
     3841 acgtcgtctc cgaggcgatg acgaacatcg ccaagcacag cggggcccgc cacggcctgg
     3901 tctacgggcg cctgcgggac ggcgcgctgg tggtggaggt ccgggatgac ggagtgggcg
     3961 gagccgaccc cgccggagga tcgggactgg ccggcctggc cgaccgcgtc gccgtcaccg
     4021 acggcgtgat gaccctgtcg agcccgcccg gcggtccgac ctcgttacgg gtggagattc
     4081 cgtgtctcat ctaggtccgt gtccggatag ccggtttggg gatgccgcgc cccctttagg
     4141 tccgcccgga tggcccgtgg gggattccgc gtccccttca ggtccgtgtc cggatcgccc
     4201 gtggggggat gccgcgccgc ggattcggag gagaccgatg gaggtttcag gtccgcgtcc
     4261 ggatggcctg cgtggagatt ccgcgctgca catgcggagg aagccggtgg aggccttatg
     4321 tctcccgtgt ggaggtgccg tgtgccgctt tcggagagat ctggcgtgcc tcgtctccgg
     4381 atagcgctgg ccgaagacgg cgtgctgctg cgtgaagggc tcgtcgggct gctcgaccgg
     4441 ttcggccatg acgtggtcgc cgcggtcggt gacggcgagg cgctcatcgc cgccgtggcc
     4501 gaacacgagc ccgacatcat cgtcgccgac gcccgcatgc cacccggctt caccgacgag
     4561 gggttgcggg ccgggctcac gctgcgggcg agccgcccgc ggctggcggt gctggtgctg
     4621 agtcagtacg tcgaacagac ctacgccgcc gaactgctcg actccggggg tggcgtgggc
     4681 atcggctacc tgctcaagga tcgcatcggt gaggtgcgcg agttcgtcga cgccgtggaa
     4741 cgggtcgcgg ccgggcacac ggtcgtcgac cccgaagtgg tgcggcaact gctcaaccgt
     4801 cgccgggaac cgctgtcccg gctgactccc cgggagcgcg aggtgctcgg tctggtggcg
     4861 gagggccgct ccaacgccgc gatcgcccgc gcgctcgtgg tcaccgaggc ggcggtcgcg
     4921 aagcacatcg ccagcatcct ggccaaactc gacctgcctc cggccgaaga cgatcaccgg
     4981 cgggtgctgg ccgtgctcgc ctacctgcgg ggacggtgac gcggaaggtg cgcttgtatg
     5041 gtgccggcgg tcggattgag ccgggggttg gagtcgggtc ggggtcggtt tgggcaccaa
     5101 gccgagctgg gcgggcaggt cgtatagccg gccgcggtca gctgccacca caagtggtgt
     5161 gatcccggta cgactacctc gaccacgggc aggccggggt ccggcacggt ctggtcgagg
     5221 gcgagcatct ccatcccccg ctcctcgacc ggcgcgcggg ccatggccac aggtctccgg
     5281 tggtaggccc gggtctgaac caggccgggg gcggtggcgg ccggggacgc gtgaggctgg
     5341 gtgcggagcg gcatcagagt cggtgtccgg cgcggtcgtt gtcgggcggg tgcagcgtcg
     5401 gccgggtcgg agtgggaccg ggagacggag agggattggg ggcggccaaa gtggatcgaa
     5461 ccgcccggaa ccctggtagc aggacgcgct tggtctcaac agaccagcaa acgacatcgc
     5521 gcgctgcagg cgagcgctca gaacgcgctg ctcagaacgc accagtcggg aggggcggga
     5581 ctcgtcagtc ggatcgcgcc ggtcaagacg gtctgatcgg gatggtctgc tcagaacgcg
     5641 ctgctcagaa cgcgccggtc gggaggggcg ggacacgtca gtcggatcgc gccggtcgag
     5701 acggtccggt cgggacgcgc cggtcaagat ggtctggtcg ggacggtctg gtcggggcgc
     5761 gctgctcaga acgcgccggt cgggaggggc gggacacgtc agtcggatcg cgccggtcga
     5821 gacggtccgg tcgggacgcg ccggtcaaga tggtctggtc gggacggtct ggtcggggcg
     5881 cgctgctcag aacgcgccgg tcgggagggg cgggacacgt cggtcggatc gcgcgggtca
     5941 agacgtttga tcgagacggt ctgttcggga tggtctggtg gggacgctct ggtcggacca
     6001 ttctggtcag aatgcgctgt tcagagcctc ggacacggtt cggatcacgt tgtcttcgtg
     6061 ggggatctgg ttgaggggcg cgtgttcggt gagggctgcg ccgatcacgc cgtcaaggcc
     6121 ggcgatcagg tcggcgagtc ggtcggggtg caccccgccg ggttccgggt agcacgtcga
     6181 accgaacacc tccggatcca gcacgtcgag gtccacgtgg acgtagaccg gccccgtcag
     6241 tccctcgaag gcccggtgca ggtcggggac gccgtaccgg cggatgccct gttcccgcag
     6301 gtaggcgtgt tccgagtccg cgcccgcccg ttcgccggcg atgatcacct ggctcggttt
     6361 cagcggccgg tccggggtca gcccggccag ctcgtcgtcg cccagcagcg cccggacgac
     6421 catcccgtgg aacgcccccg acggaagggt cgacgggctg tagacgtcgg ggtgcgcgtc
     6481 gatccacagc accgtcagcg cgtcgccgta gcggtcgtgg gcggtggcga tcgacgggat
     6541 gtccacggcg cagtcgccgc cgacggtgat gatccggtcg tcgacgccct ccagggcctc
     6601 gaaggtccgg cgctggacct cgatcaacgt gtcccgggcg cggatgccgc cggacttcgg
     6661 ggtcgtcccg ggggtcggca ccgtcaccac cgtgtcggct gtgatcaggg cggatgcgcg
     6721 gtgggcgcct tccgtcaggc ggggggctcg cgcgtgggct gagccctgcc actgcggtac
     6781 gcacaggacc gtggtcattc ccgcagtcct acccgaaagt cgggccgtcc ggcgaccttc
     6841 ggccgaggcg gggtgaccgt cgccgcgtct agcgtcgggc gcatgcgaaa aggtccccgg
     6901 cttctcggtc tgctgctgtt cgcgatgggg gtcagcggca ccgtcgatca tctggccgcg
     6961 cagcccgtga tgggcgtgct gctgaacgcg cccaaccgct ggatcttcga gcggctggag
     7021 gtcttcgagg ggcgggaggt cttcgcgaac ctgtcgctcg ccttcgtggg ggtcgtgctg
     7081 gtcgcgttct cgtttcggcg ctaaccttcc ctattgttcc ctataatagg gaacatgcag
     7141 ccctattccg tcgaacaggt cgccaatctt ctgggtcttc acgtcaagac cgtccgcaac
     7201 tacgtgcgtg acgggaagct gaaggccgtc cgcatcggca agcaatatcg catcacccgt
     7261 gaggatctgg aggccttcac cggccagccg gtcgtgcccg aggcggtgcg gcggcagcgg
     7321 ggggtggagg tgtcggcggt cgtccaggtc gacgcgatca gccgggagcg gaccgatcgg
     7381 ctgaccaacg cgctgatcgc ggcggccaac tccggcgggg aggggaagct gcgcgtcgag
     7441 gccatctacg acgaggtcag ggcctccttg aagatcatcg tcatcggggg cgcggccgac
     7501 gccgccgcgc tgctcaccat cgtcgacagc ctcacccagg agtgatcatg cacttcacgc
     7561 ttcccgccga cggcccgccg atcgccaccg aggccgacgc gctcgacgtc atcggggcca
     7621 ccttcgggca gggggtgacc tgggtggtcg tcccggccga gcggctgtcc gacgcgttct
     7681 tctccctgcg gaccgggctc gcgggcgcgg tcctgcagaa gttcgtccag tacggcttgc
     7741 gcctggccgt catcggggac gtctcccggc acgtcgcggc cagtgacgcc ttccgtgacc
     7801 tggtccggga gtgcaaccgg ggcgaccaga cgtggttcct gccgacgacg gaggagttcg
     7861 acgcccgagt cacggcacgc cgatgagccg cagcagcgcg gtggtggccg ccgccccgat
     7921 gacgatcacc gggaacggcg cccgccgcag ggccagcgcc cccgccacga ccaccccggc
     7981 gggccgcgcc cagccgccgg acgacgacgt gacgaccagg gcgaacagca gcacgagggc
     8041 cgccgtggac agcagggcct ccaggcgggg cggaagtcgt acccgaccac ggaggagagg
     8101 gcccgccagc cggaacgcga gcgttccggc ggcgagcgcc acgatcgcgg cgatcggcat
     8161 gtccatctcg ggtccctttc ggtcggggtg gcatgtgggg gtggcggggg atcgtccatg
     8221 atcacgatcg gtcgcgcggc ggctcaggct tccttcggcc gggccagcag gccgaggagg
     8281 gacagcagca ccggcagccc ggccggcagg accgtcgagg cggccagggc gatcacgccg
     8341 ccggtcagga ccgggacgcg cacgccgcgg tcggccaggg cgggcatgat cagcgccacc
     8401 aggacggcgg ggaaggcggc gtcgaggccc agggccccga cgtcgccgat cgcggcgccc
     8461 gccagcgcgc cggccacgac cgaggcgttc cacgccgcga acagggccag gccgctggtc
     8521 cagaagtagt cgtggcgttc gcgggggccc gcgcccttca gggcgaaggc cacggtctcg
     8581 tcggtgatga ggtgcactcc cggcaggcgg ttcccgacca cgtcggcgac ggcgaagccg
     8641 aaggggagca ggcgggcgtt gaggatcagg ccggtcgcga ccgccgcgac ggctccgccg
     8701 ccgcccatca ggatgccgat cgcggcgaac tgggcgccgc cggcgaacac gatcaaggac
     8761 atcaccaccg gcacccatgg gtcgaagccc ccggagacgg cgatcgcgcc gaaagacgcc
     8821 cctacgatgg cgtcggccac gcagaccagg gcgatgtccc gcagcgctgt tcgcgatatc
     8881 gaacgcatgg ccaatagaat gaacactacg gacatcgttc gtcaaggcga acaatcggac
     8941 ttgtggagcg aacggcttgg acacctccgc cgcgttgatc acgctcatcg ccgcctcgat
     9001 ccgtcgggaa cgcgaccggg tcggcctgtc gctgaccgag ctcgccaaac gggccgggat
     9061 cgccaagtcg acgttgtcgc agctcgaagc gggaggcggg aaccccagcg tggagacgct
     9121 gtgggcgctc gccgtcgcgc tgggggtgcc gatcagccgg ctgatcgatc cgccccggcc
     9181 gcatgtgcgg gtgatccgcg cgggggaggg gccggtcacc cactcggagc gggccgacta
     9241 cgcggcgacg ctgctggcct cctgcccgcc cggggcgcgg cgcgacctgt tccgcatcga
     9301 cgccgagccg ggcgaggcgc gcacctccga cccgcacaac ccgggcacgc tggagcacgt
     9361 gatcatgggg ctcgggcggg ccctggtcgg gccgaccgag gggccggtgg agctcgggcc
     9421 cggtgactac atcacctacc ctggcgacgt gccccacgtc ttccgcgccc tggaaccccg
     9481 gaccaccgcc gtgatcgtca tggagcacgt gtgaccgagc tgcactccga ctgcgcctcc
     9541 tgcgccggcc tgtgctgcgt ggagctgccg ttcacgagat cggccgactt cgccgtcgac
     9601 aaaccggcag ggaagccctg cgccaatctg accggtgact tccgctgccg catccacgac
     9661 cgcctgcgcg agagcggcta ccagggctgc gtgaccttcg actgcttcgg cgcggggcaa
     9721 cgggtgacca gggcgcggct cgacgtcgcg ctgttcccgc tgatccggca gctgcacgag
     9781 ctgctctggt acctggagga ggcctctcgg cacggccaca ccgccgacct gctcaaggag
     9841 accgaggcgc tggcggcgtc cccggtcatc gaccccctcc agatcggcga gctgcgcggc
     9901 cgggtcgggg cgcggctggg ggagatcagc gagcgggtgc gcggccggtc ccgggagatg
     9961 cgccgtgacc tggccggggc gaaactgcgc ggccaggacc tgcgcaacgt cagcctgcgc
    10021 gggcggctcc tcatcggggc cgacctgcgc ggcgcggacc tgcgcacggc cgacctgctc
    10081 ggggccgacc tgcggggggc cgacgtccgg ggcgcggacc tgcgcggcgc gctgttcctg
    10141 acccgccccc aggtcgccgc cgcccggggt gaccggacga cccgcctcga cctcgatcgg
    10201 cccggccact ggacgtagcc aggtcgcgag gacggccggg cgagccggtc cctcccggaa
    10261 tcggccgcct gggcctgctg atctacggcg tgcgctgttc ctgaccggcg cggtccggtt
    10321 cgaccacggg ccgtgacccc atctcgcgcc actccgggcg caggcccgtg agggtggtgg
    10381 tgaggaagta ggccgcgccg cagaacacca gcaccgggac caggccggtc gcggccaccg
    10441 ccgcgcccgc caggag
//
