LOCUS       NZ_BBXD01000019        10822 bp    DNA     linear   CON 08-AUG-2024
DEFINITION  Herbidospora mongoliensis strain NBRC 105882, whole genome shotgun
            sequence.
ACCESSION   NZ_BBXD01000019
VERSION     NZ_BBXD01000019.1
KEYWORDS    WGS; HIGH_QUALITY_DRAFT; RefSeq.
SOURCE      Herbidospora mongoliensis
  ORGANISM  Herbidospora mongoliensis
            Bacteria; Actinomycetota; Actinomycetes; Streptosporangiales;
            Streptosporangiaceae; Herbidospora.
REFERENCE   1
  AUTHORS   Komaki,H., Ichikawa,N., Oguchi,A., Hamada,M., Tamura,T. and
            Fujita,N.
  TITLE     Genome-based analysis of non-ribosomal peptide synthetase and type-I
            polyketide synthase gene clusters in all type strains of the genus
            Herbidospora
  JOURNAL   BMC Res Notes 8, 548 (2015)
   PUBMED   26452464
  REMARK    DOI:10.1186/s13104-015-1526-9 Publication Status: Online-Only
REFERENCE   2  (bases 1 to 331122)
  AUTHORS   Hosoyama,A., Oguchi,A., Ichikawa,N., Kimura,A., Ohshita,R. and
            Fujita,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAY-2015) Contact:Director-General Department Of
            Biotechnology National Institute of Technology and Evaluation,
            Biological Resource Center (NBRC); 2-49-10 Nishihara, Shibuya-ku,
            Tokyo 151-0066, Japan URL :http://www.nite.go.jp/nbrc/index.html
COMMENT     ##Genome-Assembly-Data-START##
            Finishing Goal                    :: Noncontiguous Finished
            Current Finishing Status          :: High-Quality Draft
            Assembly Method                   :: newbler v. 2.6
            Genome Coverage                   :: 62x
            Sequencing Technology             :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_001570545.1-RS_2024_08_08
            Annotation Date                   :: 08/08/2024 02:40:32
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,280
            CDSs (total)                      :: 8,202
            Genes (coding)                    :: 8,113
            CDSs (with protein)               :: 8,113
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 6, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 4, 5 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 21 of 89
            Pseudo Genes (incomplete)         :: 71 of 89
            Pseudo Genes (internal stop)      :: 4 of 89
            Pseudo Genes (multiple problems)  :: 7 of 89
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 18:23:16
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 172872
            Orig. end                         :: 183694
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            BBXD01000019.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10822
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="3"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10822
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10822
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:5822](-)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5822)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     misc_feature    738..752
                     /note="TFBS match to VqsM, Regulator of quorum-sensing
                     signalling systems, confidence: medium, score: 18.18"
                     /tool="antismash"
     gene            988..1515
                     /locus_tag="AW272_RS28590"
     CDS             988..1515
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="AW272_RS28590"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_157518848.1"
                     /transl_table=11
                     /translation="MTRWLCCLLLLVAACGVTSTGVQDGGPPPVISPKAAVATVYLLRD
                     GKLEPSSVAVASHSMDNVMAALFEAGTRSTLGLTSELNGLTWQTSQLTRFGAVRNDPDN
                     ADGFRLSLFVSGGRPITKTAQAQMTCTAMRQRQDIWAVKITRTDATGPVSYGEHTCREY
                     WNLAAPGRQLPR"
     gene            complement(1512..2786)
                     /locus_tag="AW272_RS28595"
     CDS             complement(1512..2786)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013133320.1"
                     /locus_tag="AW272_RS28595"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="PhoH family protein"
                     /protein_id="WP_218011113.1"
                     /transl_table=11
                     /translation="MLDTSVLLADPAALSRFDEHEVVLPVVVITELEGKRNHPELGYFA
                     RKALRFLDDLRVQHGRLDEPIPMGEGTIRVELNHSDPSGLPAGFRLGDNDTRILTVAQN
                     LASEGLDVVLVSKDLPMRIKAASIGLTAEEYRAELVAESDETWTGMAELQVTAEHVEAL
                     FEHGGTELEEARELPTHTGLRLLTSKGSALGRVLPDKSVRLVRGDREIFGLHGRSAEQR
                     IALDLLMDPEIGIISLGGRAGTGKSALALCAGLEAVLERRQHRKVIVFRPLYAVGGQDL
                     GYLPGSENEKMGPWAQAVYDTLGAVTTPEVIEEVLDRGMLEVLPLTHIRGRSLHDAFVI
                     VDEAQSLERGVLLTVLSRIGANSRVVLTHDVAQRDNLRVGRHDGVVAVVEKLKGHPLFA
                     HVTLTRSERSPIAALVTEMLEDITP"
     gene            complement(3080..3841)
                     /locus_tag="AW272_RS28600"
     CDS             complement(3080..3841)
                     /GO_function="GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017598077.1"
                     /locus_tag="AW272_RS28600"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="isoprenyl transferase"
                     /protein_id="WP_066371575.1"
                     /transl_table=11
                     /translation="MGVRDLVYKVYERRLERKLTSVQAPRHVGVIVDGNRRWARSNGHE
                     DVRHGHQKGADKISELLAWCKEAGVEYVTLWLLSTDNLSRDKGELEPLLQVIENLVGDL
                     VEQGWHVKPMGSLDLLPDHTAHVLKNAKEATSHRPGLIVNVAVGYGGRREIADAVRSLL
                     QEHASKGASIEDLVEVLDVEHIAEHLYTRGLPDPDLVIRTSGEQRLSGFLLWQSAHSEF
                     YFCEAYWPDFRRVDFLRALRAYAARNRRYGF"
     misc_feature    3886..3900
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 16.17"
                     /tool="antismash"
     misc_feature    complement(3890..3903)
                     /note="TFBS match to PerR, Peroxide regulator, confidence:
                     weak, score: 14.18"
                     /tool="antismash"
     gene            complement(3899..4411)
                     /locus_tag="AW272_RS28605"
     CDS             complement(3899..4411)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012851444.1"
                     /locus_tag="AW272_RS28605"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_245657765.1"
                     /transl_table=11
                     /translation="MACRDSAGVPYEVTLELFRDGVAYGSVGERCGWFLARLARSVAEA
                     RESQVAWDDPDDRFPGDGDEGELFGFRYRSRTDIVGGGELRCQVRLIPIWVPAKGVKGR
                     WRLIRRAYVEAWGASGAGLRAIVTSGELLMFLSSVVTEAEVRLGTQYGMERNSSLISGA
                     GTASRPR"
     gene            complement(4500..4874)
                     /locus_tag="AW272_RS44540"
     CDS             complement(4500..4874)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="AW272_RS44540"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_066371579.1"
                     /transl_table=11
                     /translation="MSDPRAQLADQQRALLAALVAGAAPPEGFDAERLRIQAGSLIAKR
                     RGLVAVARPDVVQALGSSFGREFQGYASGRPKPQGGSRADAAAFAEWVRSRGLLPEPPE
                     APQEQKRHWWRLTPKRDQSR"
     gene            complement(5001..5822)
                     /locus_tag="AW272_RS44545"
     CDS             complement(5001..5822)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: DUF692"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020545141.1"
                     /locus_tag="AW272_RS44545"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="DUF692 domain-containing protein"
                     /protein_id="WP_245657766.1"
                     /sec_met_domain="DUF692 (E-value: 1.6e-78, bitscore: 257.9,
                     seeds: 7, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MAADPPADRLGVGIGWRSEIDLTVEHLPGVEFTEVVAEQVNPERV
                     PESLRLMRERGIPVIPHGVALGLGGAERPDLARLDHLAAAAQALGSPLVSEHLAYVRSG
                     DLEAGHLLPVPRTRDALSVIVENVRIALDCLPVPLALENVAALFGWPGDELTEAQFLGE
                     LVERTGVKLLIDVANLYTNQVNLGFDAVKALDALPLEHVAYVHVAGGHGHGDVWHDTHT
                     ASPPEEVLDLLAELCARHRPPGVLLEWDADYPSDAVLSEELDRIRAAMNRG"
     gene            complement(5812..6789)
                     /locus_tag="AW272_RS45330"
     CDS             complement(5812..6789)
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:TIGR04222.1"
                     /locus_tag="AW272_RS45330"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="TIGR04222 domain-containing membrane protein"
                     /protein_id="WP_066371590.1"
                     /transl_table=11
                     /translation="MGSLLLFISAILATVLVSRQARRVKKEQRRVKFGGHPIQHSLDPY
                     SLAFLAGGPRRVVNTAVAVLVSQGRVRISRDGTVTAVRGMSGAPAERVEQEVLDLAAGV
                     NGRPLADIRRTVAVGPALTGIDYGLRGQGLVVADSALNSARRANTRLRWLIAIPGLLVP
                     VSIVTMALGWFDFVVWNVLTLVLSLLTWIIAMASAAGNAKENRSVLTQAGHAALRAAQA
                     RHPRTRELAYAHNGGNDSTIALYGLAIYTGPGHAEIQHGGGGGCGAGACGGAVGSDSGW
                     GSGSDFGGGDFGGGGGSDSGGGGGDSGGGSSCGGGGCGGGCGGS"
     gene            complement(6983..8776)
                     /locus_tag="AW272_RS28625"
     CDS             complement(6983..8776)
                     /EC_number="4.1.1.32"
                     /GO_function="GO:0004611 - phosphoenolpyruvate
                     carboxykinase activity [Evidence IEA]"
                     /GO_function="GO:0005525 - GTP binding [Evidence IEA]"
                     /GO_function="GO:0017076 - purine nucleotide binding
                     [Evidence IEA]"
                     /GO_process="GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013133322.1"
                     /locus_tag="AW272_RS28625"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="phosphoenolpyruvate carboxykinase (GTP)"
                     /protein_id="WP_066371592.1"
                     /transl_table=11
                     /translation="MSVSLQLSHPTTHSELAAWVSEIAELTEPDRIEWCDGSEAEWTRL
                     TNMLVRKGTLTRLAARPNSFHAASDPSDVARVEDRTFICSEQEEDAGPTNNWIAPGEMR
                     RTFGEVFKGSMRGRTMYVVPFCMGPLGGSISQLGVEITDSAYVAVSMKVMTRMGEEALR
                     LIEERGDFVKCVHSVGAPLSSGQQDVAWPCSDTKYISHFPETREIWSYGSGYGGNALLG
                     KKCYALRIASTMARDEGWLAEHMLILKLTPPNGKTRYIAAAFPSACGKTNLAMLQPTIP
                     GWKVETIGDDIAWMRFGDDGRLYAINPEAGFFGVAPGTGQTTNANAIKTLWGNSIFTNV
                     ALTDDGDVWWEGLTDEPPAHLTDWRGESWTPDSGEPAAHPNARFTTPAHQCPTIAREWQ
                     DPKGVPISAILFGGRRATAVPLVTEARSWQHGVFMGANVASEKTAAAEGKVGELRRDPF
                     AMLPFCGYNMGDYFAHWLGVGETKGAKLPRIYYVNWFRKDAEGRFVWPGFGENSRVLKW
                     IVERLNGQAEAVDTPIGRLPVKIDTEGLDIAEQDMELLLSVDREVWREEAALIPGHFET
                     FGDHLPEGLWEEYRALVARLG"
     misc_feature    8842..8857
                     /note="TFBS match to DasR, N-acetylglucosamine dependent
                     repressor, confidence: medium, score: 22.07"
                     /tool="antismash"
     misc_feature    8858..8875
                     /note="TFBS match to ArcA, Repressor of carbon oxidation,
                     confidence: weak, score: 16.19"
                     /tool="antismash"
     misc_feature    8997..9012
                     /note="TFBS match to DasR, N-acetylglucosamine dependent
                     repressor, confidence: weak, score: 20.04"
                     /tool="antismash"
     misc_feature    complement(9016..9031)
                     /note="TFBS match to DasR, N-acetylglucosamine dependent
                     repressor, confidence: medium, score: 23.82"
                     /tool="antismash"
     misc_feature    complement(9023..9038)
                     /note="TFBS match to RutR, Regulator of the pyrimidine and
                     purine metabolism, confidence: medium, score: 21.14"
                     /tool="antismash"
     misc_feature    9038..9053
                     /note="TFBS match to DasR, N-acetylglucosamine dependent
                     repressor, confidence: weak, score: 19.91"
                     /tool="antismash"
     gene            complement(9044..9553)
                     /locus_tag="AW272_RS28630"
     CDS             complement(9044..9553)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020545144.1"
                     /locus_tag="AW272_RS28630"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_066371593.1"
                     /transl_table=11
                     /translation="MTAEAEWFAHRLPENWFTERPEVVVDREEITVVGALPPLTVTEGL
                     PDAERAAAEDGYVDRFREETRERRIEIARQAEHKFRRKVAWGVKIGGETVMFTTLSVPV
                     MTRLRQSERQVLDTLVAAGVARSRSDALAWCVRLVGRHTDTWLADLRDALQAVDRVRAE
                     GPGGVD"
     misc_feature    9553..9566
                     /note="TFBS match to PerR, Peroxide regulator, confidence:
                     weak, score: 17.25"
                     /tool="antismash"
     misc_feature    9600..9615
                     /note="TFBS match to ColR, Phenol-responsive regulator,
                     confidence: weak, score: 19.45"
                     /tool="antismash"
     misc_feature    9610..9628
                     /note="TFBS match to FuR_variant_2, Ferric Uptake
                     Regulator, confidence: weak, score: 20.98"
                     /tool="antismash"
     misc_feature    complement(9682..9695)
                     /note="TFBS match to ANR, Anaerobic transcriptional
                     regulator, confidence: weak, score: 19.32"
                     /tool="antismash"
     misc_feature    complement(9902..9916)
                     /note="TFBS match to VqsM, Regulator of quorum-sensing
                     signalling systems, confidence: medium, score: 17.92"
                     /tool="antismash"
ORIGIN
        1 acgcgagacc ggcagcggcg cggtccgctc gaccggcatg tcggtcttcg tgttcgtctc
       61 catgaccgac gtcgaccggg tgctcgtcaa cctgcgcgcc gccctgctcc aggccggctc
      121 gctcaccatc gtcctggcgc tcaccgtcgc gctgctgtcg gcccgccagg tccttcttcc
      181 ggtacgccgc ctcggtgagg ccgcccgcgc cctgggcgcc ggccggctcg acacccggct
      241 gccggtccac ggccgcgacg aacttgccga gctcaccgcc acgttcaacg agacggccga
      301 ggcgctggag aagaccgtcc aggagctgtc ctcgctggag gcgatgtcgc ggcggttcgt
      361 cgccgacgtc tcccacgagc tgcgcacccc gctgaccacc atgaccgccg tcaccgacat
      421 gctctccgac gaggccgaac ggctgccggc cgacgcgggg caggcggtcc acctggtggt
      481 cgccgagatc gagcggctgc gctcgctggt cgacgacctg atcgaggtca gccggttcga
      541 ctccggcgcg gccgtgctcc gccgcgagat cgtcgacgtc ggcgacgaac tgaccgagtg
      601 cctggagatc cgcggctgga ccgaccaggt gaccctccgg gtgcccgacg gcctgaccta
      661 ccaggtcgac ccgcgccgct tcgacgtgat cgtcgccaac ctggtcggca acgcgctcaa
      721 acacggcaaa cggccggtga cggtgtcggc caaggtcctg cccagcgggt tggaggtccg
      781 cgtccgcgac cacggccccg gcatcccccg cgaggacctg ccccacgtct tcgaccgctt
      841 cttcaagacc ggcgccaacc gcgcccgctc gggcggcagc ggcttgggcc tgtcgatagc
      901 caaggccaac gccgaactgc accggggcac gatcacggtg aagctcgccg accccggcac
      961 ccagttcacg ttgtggatac cgcgcccatg acccgatggc tgtgttgtct gttgctgctg
     1021 gtcgccgcgt gcggcgtcac ctcgaccggc gtccaggacg gcggcccgcc gccggtgata
     1081 agcccgaagg cggccgtggc gacggtctac ctgctgcgcg acgggaagct ggagcccagc
     1141 tccgtcgcgg tggcctccca ctcgatggac aacgtgatgg ccgccctctt cgaggcgggc
     1201 acccgctcca ccctcggcct gaccagcgaa ctcaacggcc tgacctggca gaccagccag
     1261 ctcacccgct tcggggcggt gcgcaacgac cccgacaacg ccgacggctt ccggctgtcc
     1321 ctgttcgtca gcggcggacg gccgatcacc aaaacggcgc aggcccagat gacctgcacc
     1381 gccatgcggc agcggcagga catctgggcc gtgaagatca ccagaacgga cgccaccggc
     1441 ccggtgtcct acggtgagca cacctgccgc gagtactgga acctcgcggc cccgggccgg
     1501 cagcttcctc gttaaggcgt gatgtcttcc agcatctcgg tcaccagggc ggcgatcggg
     1561 gaccgttccg aacgcgtcag ggtgacgtgg gcgaacagcg ggtggccctt gagcttctcc
     1621 accaccgcca ccacaccgtc gtgccggccg acccgcaggt tgtcgcgctg ggcgacgtcg
     1681 tgggtcagca cgacccgcga gttggcgccg atgcgcgaca ggaccgtcag gagcacgccg
     1741 cgttccagcg actgcgcctc gtcgacgatc acgaacgcgt cgtggagcga ccggccgcgg
     1801 atgtgggtca gtggcaggac ttcgagcatg ccccggtcga ggacctcctc gatcacctcg
     1861 ggcgtggtca ccgcgcccag ggtgtcgtag acggcctgcg cccacggacc catcttctcg
     1921 ttctcgctgc cgggcaggta gccgaggtcc tggccgccga ccgcgtagag cgggcggaag
     1981 acgatgacct tgcggtgctg gcggcgttcg agaacggcct caaggcccgc gcagagcgcc
     2041 agcgccgact tgcccgtgcc ggcccgtccg cccagcgaga tgatcccgat ctccgggtcc
     2101 atcagcaggt cgagcgcgat gcgctgctcg gcgctgcggc cgtggagtcc gaagatctcg
     2161 cggtcgccgc gcaccaggcg caccgacttg tccgggagca cccgtccgag tgcggaaccc
     2221 ttgctggtca gcagccgcag acccgtgtgg gtcggtagtt cgcgcgcctc ttccagttcg
     2281 gtgccgccgt gttcgaacag cgcctccaca tgttctgcgg tgacctggag ctcggccatg
     2341 ccggtccagg tctcgtcgga ttcggcgacc agctccgccc ggtattcctc ggcggtgagg
     2401 ccgatcgagg cggccttgat ccgcatcggc aggtctttgg agaccagcac gacgtcaagg
     2461 ccctcggagg ccagattctg cgcgacggtc aggatgcgcg tgtcgttgtc gcccagccgg
     2521 aagccggccg gaaggccgga cggatcgctg tggttgagct cgacccgtat ggttccctcg
     2581 cccatcggga tcggctcatc gaggcggccg tgctggacac gcagatcgtc caggaatcgc
     2641 aaggctttac gagcgaagta tcccagctcg ggatggtttc gcttgccttc cagctctgtg
     2701 atcacgacga ccgggagcac cacttcgtgt tcgtcgaacc ggctcagtgc ggccggatcg
     2761 gcgagcagga cagaggtgtc caggatgtac gtgcgccggg aggtgccagg cgagtgaccg
     2821 gacttggacg ggttggacaa tgccacgcgt tctcccccgg gcgcctgggg tcggcgccct
     2881 gccttgactg gtggtgaacg gaccgggccc ggacgccccg aagggggaac cgggaaccgg
     2941 ccccacctgc gaagtcatca agacgcaaga acgggccctc tcccgaggtg agcgtggatg
     3001 ctcactcctt acggtacgtc ctgaatcccc ctgcgccaac gctccaatcc ttgccgaaag
     3061 ggggtgttca catgccgttt cagaatccgt accgacggtt acgcgcggcg taggcgcgaa
     3121 gtgcccgcaa aaagtccact ctgcggaagt ctggccagta ggcctcgcag aagtagaact
     3181 cggagtgggc gctctgccag agcaggaatc cagagagtcg ctgctctccc gaggtccgga
     3241 tgacgagatc cggatcgggc aggccacgcg tgtagagatg ctcggcgatg tgctccacat
     3301 cgaggacctc cacgaggtct tcgatgctcg cgcccttgct cgcgtgctcc tggaggaggg
     3361 agcgcaccgc atcagcgatc tcacgtcttc ctccataccc aacggcaacg ttcacaatca
     3421 gcccaggtcg gtgtgatgtt gcttctttag catttttcag gacatgggcg gtgtggtcgg
     3481 gcagcaggtc cagggacccc atcggcttga cgtgccaacc ctgttcgacc aggtcaccga
     3541 ccagattctc gatcacctgg aggagaggtt cgagctctcc cttgtcccgg gagaggttgt
     3601 cggtggagag cagccagagg gtgacgtatt cgacgccggc ctccttgcac caggcgagga
     3661 gctcggaaat cttgtccgcg cccttctggt ggccgtggcg gacgtcctcg tggccgttcg
     3721 agcgggccca gcggcggttg ccgtcgacga tcacgccgac atggcggggc gcctgtaccg
     3781 aggtgagctt tcgttcgagc cggcgttcgt acaccttgta aacaagatcc cgcacgccca
     3841 tgacgccccc cggtgattcg cttgagcccg ctgtcgcatg acaggcatgg tcaaacaatt
     3901 atcgggggcg cgatgcggtc cccgctcccg aaatgaggga gctgttacgt tccatcccat
     3961 attgggtgcc aagccggact tctgcctcag tcaccacaga actcagaaac atcaggagtt
     4021 ctcccgaagt gactatcgcc cgcaacccgg cgcccgaggc gccccacgcc tcgacatatg
     4081 cccgcctgat cagccgccac cgtcctttga cgcccttggc gggcacccag atcgggatga
     4141 gcctgacctg gcagcggagt tctccgccgc ccacgatgtc ggtacggctg cgatagcgga
     4201 acccgaacag ctccccctcg tcgccgtcac cggggaaccg gtcgtcgggg tcgtcccagg
     4261 ccacctggga ctcccgggcc tcggccaccg accgggccag ccgggccagg aaccagccgc
     4321 agcgctcccc gaccgagccg taggccaccc cgtcgcggaa cagctccagc gtgacctcgt
     4381 agggcactcc ggcgctgtcg cggcatgcca cgggatcaac cgtcaggtat gacccatcaa
     4441 cgcaccgtag tccacccacg ccacagaggg tatcccgggc acacctcgcc aaaccggtcc
     4501 taccgggatt gatccctctt tggggtaagt cgccaccagt gccgcttttg ttcttgcggc
     4561 gcttccggcg gttcgggcag cagccctctc gaccgcaccc actcggcgaa agccgccgcg
     4621 tcggctcgcg aaccgccctg gggtttcggg cggccggaag cgtacccctg gaactcccgg
     4681 ccgaaggagg acccgagcgc ctggacgacg tcgggccggg cgacggcgac cagcccgcgt
     4741 cgtttggcga tcagactgcc cgcctggatg cgcagccgtt cggcgtcgaa cccctcgggc
     4801 ggcgcggccc cggccacgag ggcggccagc agggcccgtt gctggtcggc gagctgggca
     4861 cgcgggtcag acatgactca gcaccacggt caggccggtc gaccgcggct tccacatcag
     4921 cgcgcaggag tcagccacgg ttcacggccg ccttgaagcc cgccggccgg ggcgtgcggc
     4981 cggccggtcg ggcgtgagtg tcagccacgg ttcatcgccg ccctgatccg gtcgagttcc
     5041 tccgacagga cggcgtcgct ggggtagtcg gcgtcccact ccagcagcac ccccggcggc
     5101 cggtgccggg cgcagagctc ggccagcagg tcgagcacct cttccggcgg ggaggccgtg
     5161 tgggtgtcgt gccacacgtc gccgtggccg tggccgccgg cgacgtggac gtacgcgacg
     5221 tgttccagcg gcagcgcgtc gagggccttg accgcgtcga accccaggtt gacctggttg
     5281 gtgtagaggt tggccacgtc gatcagcagc ttcaccccgg tgcgttcgac cagctcgccg
     5341 aggaactggg cctcggtcag ctcgtcgccg ggccagccga acagggccgc gacgttctcc
     5401 agggccagcg gcaccggcag gcagtcgagg gcgatgcgca cgttctccac gatcacgctg
     5461 agcgcgtcgc gggtgcgcgg caccggcagc aggtgcccgg cttcgaggtc tcccgacctg
     5521 acgtacgcga gatgttcgct gaccagcggc gacccgaggg cctgggcggc cgcggccagg
     5581 tggtcgagcc gggccaggtc gggccgttcg gcgccgccga gaccgagcgc gaccccgtgg
     5641 gggatcaccg ggatcccccg ctcacgcatc agccgcagcg actcgggcac ccgctccggg
     5701 ttgacctgct cggcgacgac ctcggtgaac tcgaccccgg gcaggtgctc gacggtcagg
     5761 tcgatctccg accgccagcc gatccccacg cccagacgat cggccggagg atcagctgcc
     5821 accgcagcca ccaccacagc ctccgccgcc gcagctcgac ccgccgccgg agtcgccgcc
     5881 accaccaccg gagtcgctgc cgccaccgcc gccgaaatcg ccgccgccga agtcggagcc
     5941 ggagccccat ccggagtcgg agcccaccgc gccgccgcag gcgcccgcgc cgcagccgcc
     6001 gccaccgcca tgctgtatct cggcgtggcc gggcccggtg tagatcgcca gcccgtagag
     6061 ggcgatggtg ctgtcgttcc cgccgttgtg ggcgtaggcg agctcacggg tgcgcgggtg
     6121 gcgcgcctgg gccgccctga gcgcggcgtg cccggcctgg gtcagcacgc tgcggttctc
     6181 cttggcgttg ccggccgccg acgccatcgc gatgatccag gtcagcagcg acaggacgag
     6241 ggtcagcacg ttccagacca cgaagtcgaa ccagcccagc gccatcgtca cgatgctgac
     6301 cggcacgagc agcccgggga tcgcgatcag ccagcgcagc ctggtgttgg cccgccgggc
     6361 ggagttgagc gccgagtcgg ccaccacgag gccctggccg cgcagcccgt agtcgatgcc
     6421 ggtgagggcc gggcccacgg cgacggtccg ccggatgtcg gccagcggac ggccgttgac
     6481 cccggcggcc aggtcgagta cctcctgctc gacccgctcg gcgggggcgc cggacattcc
     6541 ccggaccgcc gtcacggtgc cgtcgcggga gatgcgcacc cgcccctggg agacgagcac
     6601 ggcgaccgcg gtgttcacga cccggcgcgg gccgcccgcc aggaaggcca gggagtaggg
     6661 atccagcgaa tgctgaatgg ggtgcccgcc gaacttgacc cggcgctgtt ccttcttcac
     6721 ccgtcgcgcc tgacgagaga ccagcaccgt cgcgaggatc gcggagatga agagcaaaag
     6781 ggagcccatg ccgcctccag gggagactcc agagtttcgt ccgattagac gatcccacgg
     6841 gcgtcctggt tcgcgcatca gcctaaatac gggcggcttt ctccaccttc agcacgagac
     6901 ggcccgcacc tccgccgagg cgcgggccgc ccgcgtgaaa ggggggaatc ccgacgccgt
     6961 tggcgggact cctgctcgtg ggtcaaccca gtctggcgac cagggcccgg tattcctccc
     7021 agagtccttc gggcaggtgg tcgccgaagg tctcgaagtg gcccgggatc agggcggcct
     7081 cctcgcgcca gacctcgcgg tcgacgctga gcagcagttc catgtcctgt tccgcgatgt
     7141 ccaggccctc ggtgtcgatc ttgacgggca gccgtccgat cggggtgtcg accgcttcgg
     7201 cctggccgtt gaggcgttcg acgatccact tcagcacccg gctgttctcg ccgaagccgg
     7261 gccagacgaa gcggccctcc gcgtccttgc ggaaccagtt gacgtagtag atccggggca
     7321 gcttcgcgcc cttggtctcg ccgacgccga gccagtgggc gaagtagtcg cccatgttgt
     7381 agccgcagaa cggcagcatc gcgaacgggt cgcggcgcag ttcgccgacc ttgccctcgg
     7441 cggcggcggt cttctccgag gcgacgttgg cgcccatgaa gacgccgtgc tgccagctgc
     7501 gggcctcggt gaccagcggc accgcggtgg cccggcggcc cccgaagagg atcgccgaga
     7561 tcggcacgcc cttcgggtcc tgccactcgc gggcgatcgt cgggcactgg tgggccggcg
     7621 tggtgaagcg ggcgttcgga tgggccgctg gttcgccgga gtcgggcgtc caggactcgc
     7681 cgcgccagtc ggtcaggtgg gcgggcggct cgtcggtcag gccctcccac cagacgtcgc
     7741 cgtcgtcggt cagcgcgacg ttggtgaaga tgctgttgcc ccacagcgtc ttgatcgcgt
     7801 tggcgttggt ggtctggccg gtgccgggcg cgaccccgaa gaagcccgcc tccgggttga
     7861 tcgcgtagag gcgtccgtcg tcgccgaagc gcatccaggc gatgtcgtcg ccgatcgtct
     7921 cgaccttcca gcccgggatg gtcggctgga gcatggccag gttggtcttg ccgcacgccg
     7981 acgggaaggc cgccgcgatg tagcgggtct tcccgttggg cggggtgagc ttgaggatca
     8041 gcatgtgctc ggccagccag ccctcgtcgc gggccatcgt gctggcgatc cgcagggcgt
     8101 agcacttctt gccgagcagg gcgttgccgc cgtagccgga gccgtacgac cagatctcgc
     8161 gggtctccgg gaagtggctg atgtatttgg tgtcgctgca cggccaggcg acgtcctgct
     8221 gacctgacga cagcggggcg cccaccgagt ggacgcactt cacgaagtcg ccgcgctcct
     8281 cgatgagccg cagggcctcc tcgcccatgc gggtcatcac cttcatcgag acggccacgt
     8341 aggccgagtc ggtgatctcc acgcccaact gggagatcga gccgccgagg gggcccatgc
     8401 agaacgggac gacgtacatc gtccggccgc gcatcgagcc cttgaagacc tcgccgaagg
     8461 tccgccgcat ctcgccgggc gcgatccagt tgttggtggg gccggcgtcc tcctcctgct
     8521 ccgagcagat gaaggtgcgg tcttccacgc gggcgacgtc cgacgggtcg gaggcggcgt
     8581 ggaagctgtt cggccgggcc gcgagccggg tcagggtgcc cttcctgacg agcatgttgg
     8641 tgagccgggt ccattcggcc tcggagccgt cacaccattc gatccggtcg ggctcggtga
     8701 gttccgcgat ctcgctgacc caggcggcca gctcggagtg agtggtcgga tgcgaaagct
     8761 gcagtgacac ggacacgagg gcggctcctt aatgtacgtc ggggccaatt catcatccgc
     8821 gacgaatacc cggtaaatcc aattggcgta gaccagtacg tttctgcact gttaaacgcg
     8881 cgatattcgt caccacaacc gcaggtcagc gagctgggtg atgagagaag cgtacccatg
     8941 acgaaccatt tcaaatttgt gccgaccacc cctaacccat cgttgtcgta ctaaacattg
     9001 gtataggcca aagtgaatgg tccagaccat tcggtgaatt ggttcagtcc actccgccgg
     9061 ggccctcggc gcgcacccgg tcgacggcct ggagggcatc tcgcaggtca gcgagccagg
     9121 tgtcggtgtg gcggccaacc agccggacgc accacgcgag ggcgtcggag cggctgcggg
     9181 cgacccccgc ggcgacgagt gtgtcgagca cctgccgctc gctctgccgc agccgggtca
     9241 tgaccggaac ggaaagtgtg gtgaacatca cggtctcgcc accgatcttc acgccccagg
     9301 ccaccttgcg ccggaacttg tgctcggcct gccgggcgat ctcgatgcgg cgctcgcggg
     9361 tctcctcgcg gaaccggtcg acgtagccgt cttcggcggc ggcccgctcg gcgtcgggca
     9421 ggccctcggt gacggtcagc ggcggcagcg cgccgacgac ggtgatctcc tcgcggtcga
     9481 cgacgacctc ggggcgctcg gtgaaccagt tctcggggag gcggtgggcg aaccactcgg
     9541 cttctgcagt catgtaatca atattacatc gttgcgttcg actcaggtcg atattgacag
     9601 tcacaacttt ttgagagcta ctatcaaatt atggtgactt ccagatggtg ggcgctctca
     9661 gccctcgttc tctccgtcct cgtgatcggg ctcgacgcga ccgtgctcaa cgtcgcgctg
     9721 cccaccctcg ccgtcgacct cggcgccacc accggcgatc tccagtggat cgtcgacgcc
     9781 tacctcgtcg tgttcgccgc cctgctgctg cccgccgggg tcttcggcga ccggctcggc
     9841 cgcaagaaac tgctgctcgc gggcctggcg gcgttcggcg tcgcgtcggc gatcgcgatg
     9901 ctggccgact cggtcggctg gctgatcgcc gggcgggcgc tcatgggcgc cggggccgcg
     9961 ctgatcatgc cgctgtccac gtcgatcctg ccgacgatct tcccgcccgc cgaacgggcc
    10021 agggccatcg ccgtctggac cggcggcatg gccgtgggcc tgccgctcgg cccgatcgtc
    10081 ggcggctacc tgctcgacca cttctggtgg ggctcgatct tcctgataaa cctgcccgcg
    10141 gtggcgatcg cgctggtcgc ggtggtgttc ctggtgcccg agtcacgcga cgccaccgcc
    10201 cgcaggaccg acgtgcccgg cacgctgctg tcggtcgccg gcctcgccgc actcgtctac
    10261 ggcgtcatcg aagcgccgac caggggctgg agctcaccga tggtgctcgg caccatcgcc
    10321 gccgggctcc tgctcctctt cttgttcgta cgagtagagg cccgcgccaa ggccccgatg
    10381 atggacctgg ggctgttccg cgaccgcacc ttcgtctggg gcgccttcgg cgcgaccgtg
    10441 gtctctctcg gcatgatggg cgtgctgttc gtcgcgccgc tctacctcca ggcggtgcag
    10501 gggttcgacg ccatgggcac cggcctgcgc atcgtgccca tggtcgccgg gctgatggcc
    10561 ggcggcctga tcggcgaacg cctgatcaag cgcctcagcc tgcgggtcat catgggcgcg
    10621 ggcctgtccc tgctggcgct gggcctgatg gccggggcgg tgctgccggg cgactatccg
    10681 tacacggcga cctggctcgt catcaccggc gcaggagtgg gcctgacgat ggtcccagcc
    10741 atggacggcc tgctcgcgct gctgccgccc gagcgtaccg gcatgggcag cggcgtcctc
    10801 caggccctgc gccagaccgg cg
//
