LOCUS       NZ_BBXD01000018        20558 bp    DNA     linear   CON 08-AUG-2024
DEFINITION  Herbidospora mongoliensis strain NBRC 105882, whole genome shotgun
            sequence.
ACCESSION   NZ_BBXD01000018
VERSION     NZ_BBXD01000018.1
KEYWORDS    WGS; HIGH_QUALITY_DRAFT; RefSeq.
SOURCE      Herbidospora mongoliensis
  ORGANISM  Herbidospora mongoliensis
            Bacteria; Actinomycetota; Actinomycetes; Streptosporangiales;
            Streptosporangiaceae; Herbidospora.
REFERENCE   1
  AUTHORS   Komaki,H., Ichikawa,N., Oguchi,A., Hamada,M., Tamura,T. and
            Fujita,N.
  TITLE     Genome-based analysis of non-ribosomal peptide synthetase and type-I
            polyketide synthase gene clusters in all type strains of the genus
            Herbidospora
  JOURNAL   BMC Res Notes 8, 548 (2015)
   PUBMED   26452464
  REMARK    DOI:10.1186/s13104-015-1526-9 Publication Status: Online-Only
REFERENCE   2  (bases 1 to 394330)
  AUTHORS   Hosoyama,A., Oguchi,A., Ichikawa,N., Kimura,A., Ohshita,R. and
            Fujita,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAY-2015) Contact:Director-General Department Of
            Biotechnology National Institute of Technology and Evaluation,
            Biological Resource Center (NBRC); 2-49-10 Nishihara, Shibuya-ku,
            Tokyo 151-0066, Japan URL :http://www.nite.go.jp/nbrc/index.html
COMMENT     ##Genome-Assembly-Data-START##
            Finishing Goal                    :: Noncontiguous Finished
            Current Finishing Status          :: High-Quality Draft
            Assembly Method                   :: newbler v. 2.6
            Genome Coverage                   :: 62x
            Sequencing Technology             :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_001570545.1-RS_2024_08_08
            Annotation Date                   :: 08/08/2024 02:40:32
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,280
            CDSs (total)                      :: 8,202
            Genes (coding)                    :: 8,113
            CDSs (with protein)               :: 8,113
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 6, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 4, 5 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 21 of 89
            Pseudo Genes (incomplete)         :: 71 of 89
            Pseudo Genes (internal stop)      :: 4 of 89
            Pseudo Genes (multiple problems)  :: 7 of 89
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 18:23:16
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 141731
            Orig. end                         :: 162289
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            BBXD01000018.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..20558
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="terpene"
                     /region_number="2"
                     /rules="(PT_phytoene_like or phytoene_synt or Lycopene_cycl
                     or Lycopene_cycl_fung or T1TS or T1TS_KS or T2TS or TS_UbiA
                     or TS_Pyr4)"
                     /tool="antismash"
     cand_cluster    1..20558
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(PT_phytoene_like or phytoene_synt or
                     Lycopene_cycl or Lycopene_cycl_fung or T1TS or T1TS_KS or
                     T2TS or TS_UbiA or TS_Pyr4)"
                     /kind="single"
                     /product="terpene"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..20558
                     /aStool="rule-based-clusters"
                     /category="terpene"
                     /contig_edge="False"
                     /core_location="[10000:10558](-)"
                     /cutoff="20000"
                     /detection_rule="(PT_phytoene_like or phytoene_synt or
                     Lycopene_cycl or Lycopene_cycl_fung or T1TS or T1TS_KS or
                     T2TS or TS_UbiA or TS_Pyr4)"
                     /neighbourhood="10000"
                     /product="terpene"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(10001..10558)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(PT_phytoene_like or phytoene_synt or
                     Lycopene_cycl or Lycopene_cycl_fung or T1TS or T1TS_KS or
                     T2TS or TS_UbiA or TS_Pyr4)"
                     /neighbourhood="10000"
                     /product="terpene"
                     /protocluster_number="1"
     gene            complement(195..1019)
                     /gene="trpA"
                     /locus_tag="AW272_RS26785"
     CDS             complement(195..1019)
                     /EC_number="4.2.1.20"
                     /GO_function="GO:0004834 - tryptophan synthase activity
                     [Evidence IEA]"
                     /GO_process="GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="trpA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012891497.1"
                     /locus_tag="AW272_RS26785"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="WP_066370675.1"
                     /transl_table=11
                     /translation="MNASLEEFLIARRDTGRSLLVPYVTAGVTPKWTEVVHAYVDAGAD
                     AVEVGFPFSDPMLDGVTIQEASDRALAAGTTVRGILDEVATLDVDVPVVAMTYSNLVFQ
                     RSAAEFCAALRGSGLRGLIVPDSPLEEVGDLADAAAAEGLDLVLLAAPSSSRARLREIA
                     GRSRGFVYALTRMGTTGEGGEISEQAARLGRELKELTDRPVLFGFGVAGPERAAELADH
                     ADGVVVASALMRRLLDGAGAGELGEYVSSIRRALDQGARWAVHPATAPSPTT"
     gene            complement(1016..2182)
                     /gene="trpB"
                     /locus_tag="AW272_RS26790"
     CDS             complement(1016..2182)
                     /EC_number="4.2.1.20"
                     /GO_function="GO:0004834 - tryptophan synthase activity
                     [Evidence IEA]"
                     /GO_process="GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="trpB"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PALP"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1081: cysteine synthase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012891496.1"
                     /locus_tag="AW272_RS26790"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="tryptophan synthase subunit beta"
                     /protein_id="WP_066370678.1"
                     /sec_met_domain="PALP (E-value: 2.1e-55, bitscore: 182.4,
                     seeds: 145, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MDVMTDPGTTGRFGAYGGRYVPESLIPACHEIEAAFREAWSDTEF
                     RGRLTMLLSVYAGRPTPLTPAWRLSRELGITLLLKREDLTHTGSHKINNVIGQALLAQR
                     MGKRRILAETGAGQHGVATATAAALLGLEATVFMGERDIERQELNVLRMEVLGAEVVPV
                     TSGSRTLKDACNEAMRHWVSSVDDSYYCIGSVMGPHPYPWMVREFQRVIGEEAREQCAA
                     ELPYGVPDYVVACIGGGSNAVGTFAGFVDTAARLVGVEAEGGAAATFGRAGVLHGFRSL
                     VLQNDDGQVTEAHSVAAGLDYPGIGPEHAHLRDAGRARYETVNDDEVIRALRRLAHTEG
                     ILAALESSHALAWVIRAAESGGIAPGSTVMLTLSGRGDKDIASIKEML"
     misc_feature    complement(2204..2219)
                     /note="TFBS match to DasR, N-acetylglucosamine dependent
                     repressor, confidence: weak, score: 19.61"
                     /tool="antismash"
     gene            complement(2223..2789)
                     /locus_tag="AW272_RS26795"
     CDS             complement(2223..2789)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017546737.1"
                     /locus_tag="AW272_RS26795"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="PadR family transcriptional regulator"
                     /protein_id="WP_066370680.1"
                     /transl_table=11
                     /translation="MSVVRLLVLGAIRRRGRAHGYLVRSDLETWGAHEWSNATSGSIYH
                     ALKSLTGEGCLLVHETTPSEAGGPPRMEYELTGDGERAYFALLRKALSSRDPRLDLLAA
                     AVGLIDDLPRAEAIGLLRQRALAMDRWRESVTREIPPGADLDDWGPVGAVLELWLHTAD
                     SRTAWTHRMINRLEQGAYRMADDES"
     gene            complement(2802..3500)
                     /locus_tag="AW272_RS26800"
     CDS             complement(2802..3500)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020543682.1"
                     /locus_tag="AW272_RS26800"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_066370684.1"
                     /transl_table=11
                     /translation="MANERTVPLLPCRSIDEIADFYRALGFTQIYYQTRPNPYVSMKRE
                     DLQLDFFGMPGFEPADSYGTCVVAVPDTGLLFAAFAEGMRAAYGKLLVSGIPRITRPRK
                     RKNSDDVSGFSVIDPGGNWIRIFADSAGPSQPEPAPGTLAAALRNAVVQGDSRGEHVQA
                     ARILDTALRREQDTTPRADLVEALTYRAELALRADDPAHARELIVRARELAVSDAEREA
                     LDDLAGALPA"
     misc_feature    complement(3474..3476)
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            complement(3537..4103)
                     /locus_tag="AW272_RS26805"
     CDS             complement(3537..4103)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="other (smcogs) SMCOG1148: hypothetical
                     protein"
                     /gene_kind="other"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017536546.1"
                     /locus_tag="AW272_RS26805"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_066370691.1"
                     /transl_table=11
                     /translation="MDPELDAVLDAVGPRLRALRRHRGITLADLATTTGVSESTLSRLE
                     SGQRRATLELLLPLARTYDVPLDDLVGAPRTGDPRIHLTPIRRFGMTFVPLSRRPGGVQ
                     AFKMIIPSRVDPLVPAPQTHDGFEWLYVLNGHLRLVLGERDLTLPPGEAAEFDTSLPHW
                     LGSADGGVVELLILFDPQGMRTHVH"
     misc_feature    complement(3939..3941)
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            4169..4762
                     /locus_tag="AW272_RS26810"
     CDS             4169..4762
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /GO_function="GO:0008425 -
                     2-polyprenyl-6-methoxy-1,4-benzoquinone methyltransferase
                     activity [Evidence IEA]"
                     /GO_function="GO:0061542 - 3-demethylubiquinol-n
                     3-O-methyltransferase activity [Evidence IEA]"
                     /GO_function="GO:0102208 - 2-polyprenyl-6-hydroxyphenol
                     methylase activity [Evidence IEA]"
                     /GO_process="GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /GO_process="GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1089: methyltransferase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017569201.1"
                     /locus_tag="AW272_RS26810"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="bifunctional 2-polyprenyl-6-hydroxyphenol
                     methylase/3-demethylubiquinol 3-O-methyltransferase UbiG"
                     /protein_id="WP_066370693.1"
                     /transl_table=11
                     /translation="MDFWDEVYASRPVATDPRPNARLAELVAGLPPGDVLDLGCGEGGD
                     ALWLAGQGWRVTATDISAVAVERLTALAGDRVTAVRHDLGVSFPDGTFDLICAHYLHTP
                     FDLDRAAVLRAAAHALRRDGRLLVVDHGSTMPWSWNQDPDVHHPSPVEVAAEIDLDPAT
                     WTVERADALTRIATGPDGSTAEVTDNVLVIHRAP"
     gene            4752..5228
                     /locus_tag="AW272_RS26815"
     CDS             4752..5228
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017543789.1"
                     /locus_tag="AW272_RS26815"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="DinB family protein"
                     /protein_id="WP_245657741.1"
                     /transl_table=11
                     /translation="MRRDTPPPRTGIGEAEVLRAFLDYLRTSIAAKVDGVPDPQARTAA
                     VRSGTNLLGLLTHLTSVERAVFLGDDVTDWPSTFQAAPADSVADVVAHYRETVKRANDV
                     LDRCTDLGGPLPRHRPDRPAPSVRWALAHMIEETGRHAGHADILRELIDGTTGR"
     gene            complement(5236..8565)
                     /locus_tag="AW272_RS26820"
     CDS             complement(5236..8565)
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1041:
                     transcriptional regulator, SARP family"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /locus_tag="AW272_RS26820"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="AfsR/SARP family transcriptional regulator"
                     /protein_id="WP_066370699.1"
                     /transl_table=11
                     /translation="MRFQVLGQVEVLDVEIGSGPAQLGGQKQRALLALLLAAGGRIVSV
                     SRLVDELWGEEPPPKVLSSVQAYISNLRRALEPDRPARAPARILLTRPPGYALTAENLD
                     AAAFERLVGEAQKILTTDPAHALDLLRSAGELWRGEPYEDVAAMAPGLAAEAARLTEVW
                     LLATEDRFRAELALGVHNRLVGEIERMVTAHPLREAGWGLLAVALYRSQRQGEALDALR
                     RARQVLADELGVDPGPELRRLEAAVLNHEPSLDAMPAPAKAVLVPVPPVAATGLVGRDD
                     WLARLTGHLVDTSAGRGTLVLVTGEPGVGKTGLARALTATAAAMGLRTGWGRCEETAGA
                     PALWPWSQALAPLAEASATGQWLPGWDGTAADLDTVAFRLAEATAALLRETGPALLVLD
                     DLHWADGDTLHLVRRLGAMLATLPVVLLLTSRDAEADLSPELAEALADLARADLVRVGL
                     RGLDQAGVRDYVALRHGVEIPDEVAAALRERTNGNPFFIGELVQLLAEERRLTDPEAPG
                     ALRVPDGVRDVVRRRIDQLPDDVEPLLSTAAVYGRTFDADLVEAASGLTGEAALTATEA
                     ALLAGLIEEEGDGHRFTHALVREAIYARLSPGRRRLLHAALAGEIEARSGRVRETRLTE
                     LAHHYGRAAAEHARAAWTYATRASAVAALQPAPAEAARLQEAALSALSRDRTATDADRY
                     EVLLGLALARKRAGYERQAWESAREAAEVALAGGDVVAAARAAVTTTTDAIWSWREYQV
                     VDVAATALFERLARELPAGHEALRARLLAALAGEIYYGADTADRSVALSTEAEALARAH
                     GTRDDLARVLELRHVAYERPQLLDERLAASRELVEMAESADDPAAQARALVFRGRDQIE
                     HGDVGAGLRHYRDARELAERHALAPALVVLTWADAIVAVARGRFEEAEQAIADAREFHS
                     GTTVPGAAEIPIALTATLNLARGTLPAIEPLLAEAAAATGLSLLRDWHALALVRSNRTE
                     QARTALGPWRQQPEVPRNYMWLTHMAIRAELWSALASPDAAHDLYADLAPYAGRIVIGG
                     TGITFAGFAAHHVGLLARARGDLDTALDHLRAALRDNEESGFRPFAAATARELAATLQS
                     RK"
     gene            8670..9380
                     /locus_tag="AW272_RS26825"
     CDS             8670..9380
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF013936.4"
                     /locus_tag="AW272_RS26825"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hemerythrin domain-containing protein"
                     /protein_id="WP_066370702.1"
                     /transl_table=11
                     /translation="MTSQTGQRLHGPGLTDVRMMRVAHSNFRRELGLSPAAIRAVAGGD
                     RRRTTIVADHADLFLSLLHHHTIEDDLLWAALLERVPAELAPLVQVMQSQHEHVAALLD
                     RSHAALGAWRRTASAPDGDALAVVLSDLCTALFEHLHAEETHLLPIMARHITPAEWEEF
                     TVQGMSSIPKRLMFLGFGMMLYEGDPEAIEIELRKLPAPLRRLLPPLGRRAYRRYARRV
                     HGTPAPPRGIGVIA"
     gene            9410..9853
                     /locus_tag="AW272_RS26830"
     CDS             9410..9853
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /locus_tag="AW272_RS26830"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="WP_066370708.1"
                     /transl_table=11
                     /translation="MTAQTIDLAAARARNEDVWRRSSTLLYARQIDAFVELWCEDASYE
                     AALPVAGLPAVITGHEALHAAFTGLVSAAKSITVHDVRFHQTDDPDVAIIEERMVGELA
                     DGDRYENRLIIRVTFRDGRIAHMLEYYGQFAHQDLLRKLGFTA"
     misc_feature    complement(9414..9429)
                     /note="TFBS match to LexA_variant_1, Repressor of DNA
                     damage response, confidence: weak, score: 19.75"
                     /tool="antismash"
     gene            complement(10001..10558)
                     /locus_tag="AW272_RS26835"
     CDS             complement(10001..10558)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     terpene: T1TS"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1052: Terpene synthase/cyclase metal-binding domain
                     protein"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="AW272_RS26835"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_066370711.1"
                     /sec_met_domain="T1TS (E-value: 8.5e-25, bitscore: 82.1,
                     seeds: 237, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKVPGHLEAACPTGPGTSTARPAHPDTDPTPPDAEDGGPRFRIPW
                     PARLNPEVERMRRRSLQWAASFGIVTGEQEIRRLDEPRFDLLTGYAFPDARGLGAQLAS
                     DWMYWFFPFDDWFDGPLGEDPALARTVLDPMIRFAYGEAGALPISAPPLVRAYADLCAQ
                     PHGHVAHLADQVRSRPAHRVVP"
     misc_feature    complement(10645..10658)
                     /note="TFBS match to PsrA, Oleic acid responsive regulator,
                     confidence: weak, score: 18.37"
                     /tool="antismash"
     gene            complement(10718..11242)
                     /locus_tag="AW272_RS26840"
     CDS             complement(10718..11242)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="AW272_RS26840"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_066370714.1"
                     /transl_table=11
                     /translation="MERARQLAGEVLICLFLVILGTGVYLMIHFVPADAEIFYDGSYLP
                     LQGVHMSEPYEYETALRGSLDIEGGLLSRRLHQSASIMFLACTVVWALLGRYREAAVVF
                     ALGLLIAFSGSRLAGGHGPESLWWGVHLVAALAVVVILLRGSWQEARINRRTPGLVALA
                     LAATAFLVIVL"
     gene            complement(11258..14062)
                     /locus_tag="AW272_RS26845"
     CDS             complement(11258..14062)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="AW272_RS26845"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_066370716.1"
                     /transl_table=11
                     /translation="MTVDDIRTEIRNLVANRDAEGLALRLPELTDAERAAVAAELPGLL
                     AELRDSPLLAVSLDGDVFDWTDDSQVIGSSEVLMMVGAATISGPAAVVTWLTKRDFNPR
                     WSTAPSPHRLVKAVSGRPAAWRADLAVRLARRIRRPDDRIASLALALLRDGVVTPPDHE
                     PLVVAWLSAVGVAKDPLTPQLLAKIFEADGAGRALAAETLTPKPSRWLALTVRELPREQ
                     IIDGCVGRFLRGGDVPDLRFFVRLHTLVAPTPEESAGRLRDYLRLLPAAPGPVAELAWQ
                     QVHQARLDPADVAEAIEAMTFRPEAKLAVKGLTWLDRDLAAAPERVAGHVTALTNAYAH
                     GSPEVRDRAVALTLKHAGHLTDPAPILEAAELLPPASARQLFERFTGGWTADFTPGRLP
                     EVTVPGPFPVSGLESGPPEEIGWIDVEAWLAAVVRAAHTNPGPLRRTLARRFGEHLRPV
                     LYDALTWPSPRDWCTALARELVNPGEDPGPPAARRPAHQKPVSEEQRTDAEHAFPGLSG
                     DLGQSLYGGMVSVAATAFQLFPGLRTVVPLHALPGYLQEEVLGTADIYDPATRPEPAPW
                     NRLPDPGECARPHTFLLSRLDELLRAAKAGVLPPVLLATPTRADGHLDPGPLLDRLAVY
                     AESSHEPLPLDLAQALLRLPPDLGPDLAERARNSGSAAVARWFERGAPPPPEVELTWTL
                     ALGSRVVPYDDHVVDQRGIADVSWKAAEPLGIAPLDTLLTMPETRPYRRESRHYWPGMT
                     PHHRELAAAHLASGLIDRNDPPDIAIGDLHDLALAEGASGPATALVLAYFLTEEGDDAV
                     PPLLQLAAKDALPAAETGRLLGMLGRRTRYEAGPALPSLELAARQGAHAEVWTILRHFL
                     AEFLPREGERALALHTDAVVLAVDVATWADARGEIPEVAALAKKRGSTSLGRACRSLDR
                     LLNS"
     misc_feature    complement(13409..13411)
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            complement(14112..17309)
                     /locus_tag="AW272_RS26850"
     CDS             complement(14112..17309)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="AW272_RS26850"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_066370718.1"
                     /transl_table=11
                     /translation="MTLWTEIRDLVTGNRVTPLVDRLVTLTEEERAELGGQLPGLLKEL
                     RRPPADDPDDVDELVGEQLDTSAGALLLTGVGVITGPAAAVTWMTGRDVNRRWGGTVDV
                     AQVCRVAATRPPEWRRDVAVRLAQRVRRPADRLAPLAVALLRESGATPPDHDPLVAAWL
                     TVPSLAGDPLTSVLLPHVFTADGAGRALREERLDPGPTRWLAAAARELPREQALDGCVS
                     RFLRGGDVQDLRFFVRLHTLLDPTPEESAPRLRDYLRLLPSAPGPVAELAVGQVRAAMP
                     LDHADLVEAVEALTFRAEAKFAARGLRWLDQTIRATPEVAADFVGALTTAYTHASFDVR
                     NRSTELTLKHAGLFADHAEAILDGARHLPADLGARLAERFGGEAPAEDPQERKEFSPLP
                     EVPARESFPKPSIFPNYHERWIGWERWMAAFVGGVAENRTNLRRRLKPHVEQQTHRQNR
                     DQITDPDEWQTVLAEELVTPGALPEIPPRGPEKFWDSASHSVSARLVNRGEEIGLNPPK
                     RRFAVAGSGWRPGRYLDDDTPLRVVFITWTSDDGPAGAVADEAGQPIPYANGRIYLYGQ
                     PSGDDRTPDPAPDEDDEYDGDFEEDFDDAVPDRRASRPVEPGVLYDDSDEAMPQDSLRG
                     VYERLAELGVNPDRITAMRKGLPVPPPGPDEPLVAVTVAFVPSRLRSFQRRELPPLHEW
                     RRRHRLPHPEHLSPPSDFLVTRYAELYGALRTGTLPPVLLATPTWMTGHLDPDVLVDRL
                     ETCAAAGVEPLRADLTQALLRLPRGGHPAAADRAAKIDSVAARRAHEWLAGPGMPDPEC
                     GFQWTHMVNASMVPFGDGEPEHFTEVRLTPVLRATPTGDELIDGALLRQPPDGSFDEMG
                     SGIAGWPAMLPSHREVTAVNMLPFVLRSRWYASVTPAELSRLAAADGPLGDATAVTIAV
                     LLAGGVPGTIPVVLRMAARGDLPAEAIGRQLALALRRTWLETRPAIAALTELAEAGGHH
                     EVWRILRALLPGMLPGEGRRITVTHTELVAFAADVARWTGASGEIPIVAEFARSRRTIR
                     FVHECKRLHTQLTGATS"
     gene            complement(17306..18634)
                     /locus_tag="AW272_RS26855"
     CDS             complement(17306..18634)
                     /GO_function="GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020546862.1"
                     /locus_tag="AW272_RS26855"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="SWIM zinc finger family protein"
                     /protein_id="WP_066370720.1"
                     /transl_table=11
                     /translation="MTSATQAYTYAAPSSLVDGRLGLATSGGRALSGPALAPRFFSGVV
                     TQAAPAAAALLGVADVALARYHQPQARPGWVRDPVVTCNGDRLRFESFSACGGVYARLD
                     LLGGALDGEVFDRGTTNVDVNAPLREALARIGARDPLHLGVGLDELVVTTMDGAVVEKK
                     VPLPERWLRGFAEVQVIASGMDQRGELAGMTAVRFLRGLPKKGTVWVTQSGRDLKVADR
                     QVPGAVQLAGPHRLGTLLPLLRFAKTLRVYGPADAASSAWELELPGMRYTLTLSPERWR
                     GFSGEGAVLGDLATDEAENDADVVGMLLNFEPEVEIGLLADRAGIPADRVRAALTQLGV
                     AGRVGYDLAEAAHFHRELPYDKDNVILLNPRLAKARKLVEAGAVRLEGELATVHTSGGA
                     RRVSLPDGSCTCEWWWDHRGSRGPCKHVLAARIVARAAVEVSR"
     misc_feature    complement(18122..18124)
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            complement(18685..19629)
                     /locus_tag="AW272_RS26860"
     CDS             complement(18685..19629)
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF036726.4"
                     /locus_tag="AW272_RS26860"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="acyltransferase domain-containing protein"
                     /protein_id="WP_066370722.1"
                     /transl_table=11
                     /translation="MDLDDTAARLGVSVEDVDRVHRLAGDLPSAPLPAKADAPAMLDRL
                     AVRPDDAAEIMEGWPDPGSPLWTPELRWLLDRSIALVRADLGGGGWLLPGPELPRDRGP
                     AWRHLYVYAFLALADVAMAYHRDHGVDEAVSWLSLADLGRNLAVDRRMNREGWPVMQAW
                     LTLHARGAVYEMGRLQFHRGGTTLDLHIPESGPMTPEAIDASLDQARVFFPRHFPDERY
                     TAFGCGSWLLDPQLQEYLPEDSNIVRFQRRFELEPYEESEGPDADVEVLRFVFRTLTTP
                     LDRLPRDNVLQRAIVDHLRAGRRWHWRRGSFPI"
     gene            complement(19670..19816)
                     /locus_tag="AW272_RS42445"
     CDS             complement(19670..19816)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="AW272_RS42445"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_157518805.1"
                     /transl_table=11
                     /translation="MVLVVSGGTVWMGAHASWIAATRDHDTATLGIVGTLMALGSLIAV
                     SAV"
     misc_feature    complement(19999..20012)
                     /note="TFBS match to ANR, Anaerobic transcriptional
                     regulator, confidence: weak, score: 18.18"
                     /tool="antismash"
ORIGIN
        1 gtcgagggga tactccgcgc tggccggcgt cacgtaggtg ccgcgaccgg cccgctggac
       61 gatcaggccc tcgccgctga ggacctgcag tgcctgccgc agcgtcatca tcgtcacgcc
      121 gtaggaggcg ctgagctcac gttgcgcggg cagcgcctcg cccgcgcggt agtggcccga
      181 cctgatcttc tccgtcaggt cgtcggcgat ggcgcggtag cggggtgtac tgcccatctg
      241 gctccctggt cgagtgcgcg tcgaatgctc gacacatatt ctcccagctc gcccgccccc
      301 gcgccgtcga ggagccggcg catcaacgcc gaggccacca cgacgccgtc ggcgtgatcc
      361 gccagttcgg cggcccgctc cgggcccgcc accccgaagc cgaacaggac cggccggtcg
      421 gtgagctcct tcagctcacg gccgagccgg gcggcctgct cggagatctc gccgccctcg
      481 ccggtggtgc ccatgcgggt cagggcgtac acgaaacccc ggctgcgccc ggcgatctcg
      541 cgcagcctcg cccgggacga cgacggcgcg gcgagcagca ccaggtccag gccctcggcc
      601 gccgccgcgt ccgccaggtc gccgacctcc tccagcggcg agtcgggcac gatcagcccg
      661 cgcagcccgc tcccgcgaag cgccgcgcag aactcggcgg cgctccgctg aaagaccagg
      721 ttgctgtacg tcatcgcgac gaccggcacg tcgacgtcga gcgtggcgac ctcgtcgagg
      781 atccccctga ccgtggtccc ggccgcgagc gcccgatccg aggcctcctg gatcgtgacc
      841 ccgtccagca tcggatcgga gaaggggaag cccacctcga cggcgtcggc tccggcgtcg
      901 acgtaggcgt ggacgacctc cgtccatttc ggggtgactc cggcggtgac gtagggcacc
      961 agcagcgaac gccccgtgtc gcggcgggcg atcaggaact cttccaggga cgcgttcaca
     1021 gcatctcctt gatggaggcg atgtccttgt cgccacggcc ggagagggtc agcatcacgg
     1081 tcgaccccgg cgcgatccca ccggactcgg cggcccggat cacccaggcc agagcgtggg
     1141 aggactccag cgcggccagg atcccctcgg tgtgggccag gcggcgcagc gcccggatca
     1201 cctcgtcgtc gttcacggtc tcgtagcggg cccgcccggc gtcgcgcaga tgggcgtgct
     1261 cggggccgat tcccggatag tcgaggccgg ccgcgaccga atgggcctcg gtgacctggc
     1321 cgtcgtcgtt ctggaggacc agcgagcgga agccgtgcag caccccggcc cggccgaagg
     1381 tcgccgccgc gccgccctcg gcctcgaccc cgacgagccg ggcggccgtg tcgacgaagc
     1441 ccgcgaacgt gcccacggcg ttggagccgc cgccgatgca ggcgaccacg tagtcgggca
     1501 cgccgtaggg gagttcggcg gcgcactgct cgcgggcctc ctcgccgatc acccgctgga
     1561 actcgcggac catccacgga taggggtgcg ggcccatcac cgatccgatg cagtagtagg
     1621 agtcgtccac cgaagacacc cagtggcgca tcgcctcgtt gcaggcgtcc ttgagggtgc
     1681 ggctcccact ggtgaccggc acgacctccg cgccgagcac ctccatccgc aggacgttga
     1741 gctcctgccg ttcgatgtcg cgctcgccca tgaacacggt ggcttcgagg cccagcagcg
     1801 ccgcggcggt ggcggtggcg acgccgtgct ggccggcgcc ggtctcggcc aggatccgcc
     1861 gcttgcccat ccgctgggcg agcagcgcct ggccgattac gttgttgatc ttgtgggagc
     1921 cggtgtgggt gagatcttcg cgcttgagca gcagcgtgat gccgagttcc cgcgacagcc
     1981 gccaggccgg ggtcagcgga gtgggccggc ccgcgtagac cgagagcagc atggtgagcc
     2041 tgccgcggaa ctccgtgtca ctccaggcct cccggaacgc cgcctcgatc tcgtggcagg
     2101 ccgggatcag cgactccggc acatatcgcc cgccgtaggc gccgaacctc ccggtggtcc
     2161 cggggtcggt catcacgtcc atgatcgcct tctctcatga gctactgttc tagaacagta
     2221 gctcatgatt cgtcgtcggc catccggtac gcgccctgtt cgaggcgatt gatcattcga
     2281 tgtgtccagg cggtgcggct gtcggcggtg tgcagccaga gttcgagtac ggccccgacc
     2341 ggcccccagt cgtcgaggtc ggcgccgggc gggatctccc gggtgacgct ctcgcgccac
     2401 cggtccatcg ccagcgcgcg ctgcctgagc aggccgatgg cctcggcgcg gggaaggtcg
     2461 tcgatgagcc cgacggccgc cgcgagcagg tcgagccggg ggtcacgact ggagagcgct
     2521 ttgcgcagca gggcgaagta ggcccgctcg ccgtcacccg tcagttcgta ttccatgcgc
     2581 gggggcccgc ccgcctcgct gggggtggtc tcgtggacga gcaggcagcc ctccccggtc
     2641 agggacttca gggcgtggta gatcgagccg gacgtggcgt tggaccactc gtgcgcaccc
     2701 caggtctcca ggtcggagcg gaccagatag ccgtgggccc ggccccgcct ccggatcgcg
     2761 ccgagcacca gcagccggac cacggacatg tgaggttccc ttcaggccgg gagtgcgccg
     2821 gccagatcgt cgagtgcttc ccgctcggca tcggacaccg cgagctcccg cgcccggacg
     2881 atgagctcac gggcgtgcgc cgggtcgtcg gcccgcagcg cgagctcggc ccggtaggtc
     2941 agcgcctcca ccaggtcggc ccgcggcgtg gtgtcctgct cgcggcggag cgcggtgtcc
     3001 aggatcctgg ccgcctggac gtgctcgccc cgggaatcgc cctgcacgac ggcgttccgc
     3061 agcgcggcgg cgagcgtgcc cggggccggt tccggctggg acggcccggc cgagtcggcg
     3121 aagatcctga tccagttgcc acccggatcg atgaccgaga acccggacac gtcgtcggag
     3181 ttcttccgct tgcgcggacg ggttatccgg gggatccccg acaccaggag cttgccgtag
     3241 gccgcccgca tcccctcggc gaacgccgcg aacagcaggc ccgtgtcggg caccgccacc
     3301 acgcacgtcc cgtaggagtc ggcgggctcg aagccgggca tcccgaagaa gtcgagctgg
     3361 aggtcctccc gcttcatcga cacgtagggg ttggggcggg tctggtagta gatctgggtg
     3421 aagcccagcg cacggtagaa gtcggcgatc tcgtcgatcg accggcaggg cagtaacggg
     3481 acggttctct cgttggccac gtgtctcctt cgactagcca aatttggcta ctgaggtcag
     3541 tgtacgtgtg tccgcatccc ctgcgggtcg aacaggatga ggagttcgac caccccgccg
     3601 tcggcgctgc ccagccagtg cggcagggac gtgtcgaact cggccgcctc gccgggcggc
     3661 agcgtcaggt cgcgctcgcc gagaacgagc cgcagatgcc cattgagtac gtagagccat
     3721 tcgaagccgt cgtgggtctg cggcgccggg accagcggat ccacccggga cgggatgatc
     3781 atcttgaagg cctgtacccc gcccggccgc cgggagagcg gcacgaaggt catcccgaac
     3841 ctccggatcg gcgtgaggtg gatccggggg tcgccggtgc gtggggcgcc gaccaggtcg
     3901 tccagcggga cgtcgtaggt gcgggccagc ggcagcagta actccagggt cgcccggcgc
     3961 tgcccgctct ccagccggga cagcgtgctt tccgacactc ctgtcgtcgt cgcgaggtcg
     4021 gcgagggtga tgccgcgatg acgccgcagt gctcgcagtc gcgggccgac cgcgtcgagt
     4081 accgcgtcga gttcagggtc catggagcca tcttgccgaa acagcaagat ttcgtgccag
     4141 atcggtatgt cccgtcgaga ctgagctcat ggatttctgg gatgaggtct acgcctcccg
     4201 gccggtggcc accgacccgc ggccgaacgc ccggctcgcc gaactcgtcg cgggcctgcc
     4261 gcccggcgac gtgctggacc tcggatgcgg cgagggcggc gacgcgctgt ggcttgccgg
     4321 tcaggggtgg cgggtcaccg ccaccgacat ctcggccgtg gcggtcgagc ggctcacggc
     4381 gctcgccggt gatcgtgtca ccgccgtccg gcacgacctg ggcgtgtcgt tccctgacgg
     4441 tacgttcgac ctgatctgcg cccactacct tcacacgccg ttcgacctcg accgggcggc
     4501 cgttctgcgc gcggccgcgc acgcgctgcg ccgggacggg cggctgctgg tcgtcgacca
     4561 cggctcgacc atgccgtggt catggaacca ggaccctgac gttcaccacc cgagcccggt
     4621 ggaggtcgcg gcggagatcg atctcgaccc ggcgacgtgg acggtcgagc gggccgacgc
     4681 gctcaccagg atcgcgaccg gcccggacgg cagtaccgcc gaagtcaccg ataacgtgct
     4741 ggtcatccac cgtgcgccgt gacaccccgc cgccccgcac cgggatcggc gaggccgagg
     4801 tgctgcgcgc attcctcgac tacctccgga cctcgatcgc cgccaaggtc gacggcgtcc
     4861 ccgatcccca ggcgcggacg gccgccgtgc ggtcgggcac gaacctgctc ggactgctca
     4921 cccacctgac gtccgtcgaa cgcgccgtct tcctcggcga cgacgtcacc gactggccgt
     4981 cgacgttcca ggccgcaccc gccgacagcg tggccgacgt cgtggcccac taccgcgaga
     5041 cggtgaagcg cgcgaacgac gtgctcgacc ggtgcaccga tctcggcggc ccgctcccac
     5101 gacaccggcc ggaccgcccc gcgcccagcg tccgctgggc cctcgcgcac atgatcgagg
     5161 agaccggccg ccatgcgggc cacgcggaca tcctccgcga actgatcgac ggcacgaccg
     5221 gccgctgacc ccgcttcact tgcgagattg cagggtggcg gccagttcgc gggcggtcgc
     5281 cgcggcgaag gggcggaacc cggactcctc gttgtcccgc agcgccgccc gcaggtggtc
     5341 gagggcggtg tcgagatcgc cgcgggccct ggccaggaga ccgacgtggt gggcggcgaa
     5401 gccggcgaac gtgatgccgg tgccgccgat cacgatccgg ccggcgtagg gggccaggtc
     5461 ggcgtacagg tcgtgggcgg cgtcggggga ggcgagggcc gaccagagtt cggcccggat
     5521 cgccatgtgg gtcagccaca tgtagttgcg cggcacctcc ggctgctgcc gccacgggcc
     5581 gagcgcggtc cgggcctgct cggtccggtt cgagcggacc agggccaggg cgtgccagtc
     5641 gcggagcagg gacaggccgg tggcggcggc ggcctcggcc agcaggggct cgatcgccgg
     5701 gagggtgccc cgtgccaggt tcagggtcgc cgtcagggcg atcgggatct cggcggcgcc
     5761 gggcacggtc gtaccggaat ggaactcacg ggcgtcggcg atggcctgct ccgcctcctc
     5821 gaaccggccg cgggccacgg ccacgatcgc gtcggcccag gtgaggacga cgagcgcggg
     5881 ggcgagggcg tgtcgctcgg ccagttccct ggcgtcgcgg tagtggcgca ggcccgcgcc
     5941 gacgtcgccg tgctcgatct ggtcacggcc gcggaacacc agcgcgcggg cctgggcggc
     6001 ggggtcgtcg gccgactcgg ccatctcgac cagttcgcgt gaggcggcca ggcgctcgtc
     6061 gaggagctgc gggcgttcgt aggcgacgtg gcggagttcg aggacgcggg ccaggtcgtc
     6121 tctggtgccg tgggcacggg ccagggcttc ggcctcggtc gacagggcca cggagcggtc
     6181 ggcggtgtcg gcgccgtagt agatctcgcc cgccagggcg gccagcagcc gggcccgcaa
     6241 cgcctcgtgc ccggcgggca gctcccgggc gagccgctcg aacagggcgg tggcggcgac
     6301 gtccaccacc tggtattcgc gccagctcca gatggcgtcg gtggtggtgg tgaccgccgc
     6361 gcgggccgcc gccaccacgt cgccgcccgc cagggcgacc tcggccgcct cgcgggcgga
     6421 ctcccaggcc tgacgctcgt agcccgcgcg tttgcgggcg agcgcgagcc cgagcaggac
     6481 ctcgtagcgg tcggcgtccg tcgcggtgcg gtcgcgcgac agcgccgaca gcgcggcctc
     6541 ctggaggcgg gcggcctcgg cgggggcggg ctgcagggcg gccacggccg aggccctggt
     6601 cgcgtaggtc caggcggcgc gggcgtgctc ggccgcggcc cggccgtagt ggtgggccag
     6661 ttcggtgagg cgcgtctcgc gcacccggcc ggaccgcgcc tcgatctccc cggccagcgc
     6721 cgcgtgcagg agcctgcggc ggcccgggga cagccgcgcg tagatcgcct cccggaccag
     6781 cgcgtgggtg aaccggtggc cgtcgccctc ttcctcgatc aggcccgcga gcagcgcggc
     6841 ctcggtggcg gtcagcgcgg cctccccggt cagccccgac gccgcctcga ccaggtcggc
     6901 gtcgaaggtc cgtccgtaca cggcggccgt cgacagcagc ggttcgacgt cgtcgggcaa
     6961 ctggtcgatc cgccgccgga cgacatcccg aacgccgtcg gggacgcgca gcgcccccgg
     7021 ggcctccggg tcggtgagac gtcgctcctc cgccagcagc tggaccagtt ccccgatgaa
     7081 gaacgggttg ccgttggtgc gttcccgcag cgccgccgcg acctcgtcgg ggatctcgac
     7141 accgtggcgg agcgcgacgt agtcgcggac gcccgcctgg tcgagcccgc gcagccccac
     7201 ccggacgaga tcggcgcggg ccaggtcggc cagcgcctcg gccagttccg gtgagaggtc
     7261 ggcctcggcg tcgcggctgg tcagcagcag gacgaccggc aacgtggcca gcatcgcgcc
     7321 gagccgcctg accaggtgca gggtgtcgcc gtcggcccag tggaggtcgt cgaggaccag
     7381 cagggccggg ccggtctcgc gcagcagcgc ggcggtggcc tcggccagcc ggaaggccac
     7441 cgtgtcgaga tcggcggccg tgccgtccca accgggcagc cactggcccg tcgcggacgc
     7501 ctcggccagg ggcgccagcg cctgcgacca cggccacagg gccggcgcgc cggcggtctc
     7561 ctcgcagcgt ccccatccgg tgcgcaggcc catcgcggcg gcggtcgcgg tcagcgcgcg
     7621 ggccagcccg gtcttgccga cgccgggttc acccgtcacc agcaccaggg ttccccggcc
     7681 cgccgaggtg tcgacgagat gcccggtcag gcgggccagc cagtcgtcgc ggccgaccaa
     7741 gccggtcgcc gcgaccggtg gcacgggaac caggacggcc ttcgcggggg cgggcatggc
     7801 gtcgagtgaa ggttcgtggt tcagcacggc cgcttcgagc cgccgcagct caggacccgg
     7861 atcgacgccg agttcgtcgg ccaggacctg ccgggcccgg cgcagcgcgt cgagcgcctc
     7921 gccctgacgc tgggagcggt agagggcgac cgcgagcagc ccccagcccg cctcgcgcag
     7981 cggatgcgcg gtgaccatcc gctcgatctc gccgaccagc cggttgtgca ccccgagggc
     8041 gagctcggcc cggaaccggt cttcggtcgc gagcagccag acctcggtca gccgggccgc
     8101 ctcggcggcg agcccgggag ccatggccgc gacgtcttcg tagggctcgc cgcgccacag
     8161 ttcgccggcg ctccgcagca ggtcgagggc gtgggcggga tcggtcgtga ggatcttctg
     8221 cgcctcgccg accaggcgct cgaaggcggc ggcgtcgaga ttctcggccg tcagggcgta
     8281 gccgggcggg cgcgtgagca ggatccgggc cggcgcccgc gcgggccggt cgggttcgag
     8341 ggcgcgccgg agattcgaga tgtaggcctg caccgaggac agcactttcg gcggcggctc
     8401 ctcaccccac aactcgtcga cgagacggga gaccgacacg atccggccgc cggcggcgag
     8461 cagcagcgcc agcagggcac gctgcttctg cccgccgagc tgcgccggac cgcttccgat
     8521 ttcgacgtcg agaacctcga cctggcccag cacctgaaac cgcacccggc aaagctaacg
     8581 ggcccacggg tcccgtccaa gtccggccag catgacatcc aagtagggcg gcgcaggctg
     8641 tgcacatccc accgagaaag gagagggcaa tgacgagtca gaccgggcag cggctccacg
     8701 ggccggggct caccgacgtg cggatgatgc gggtggcgca ctccaacttc cgccgcgagc
     8761 tgggcctgtc cccggccgcg atccgcgcgg tcgccggggg agaccgccgc cggacgacga
     8821 tcgtcgccga ccacgccgac ctgttcctgt cgctgctgca ccaccacacc atcgaggacg
     8881 acctgctgtg ggcggcgctc ctggagcgcg tccccgccga gctcgccccg ctggtccagg
     8941 tgatgcagag ccagcacgag cacgtggccg ccctgctgga ccggtcgcac gcggcgctcg
     9001 gcgcctggcg gcgcaccgcg tccgcacccg acggcgacgc cctggccgtg gtcctgtcgg
     9061 atctgtgcac ggcgctgttc gagcacctgc acgccgagga gacccacctc ctgccgatca
     9121 tggccaggca catcacgccg gcggagtggg aggagttcac cgtccagggc atgtcctcga
     9181 tcccgaaacg cctcatgttc ctcggcttcg gcatgatgct ctacgagggc gaccccgagg
     9241 cgatcgagat cgagctccgc aagctccccg caccactgcg ccgcctgctg cccccactgg
     9301 gccgccgcgc ctaccgccgc tacgcccgcc gcgtccacgg cacccccgcg ccgccgcgcg
     9361 gcatcggcgt catcgcctga gtcaccctca acaccgaagg agttccacca tgaccgcaca
     9421 gaccatcgac ctcgccgccg cccgagcccg gaacgaggac gtctggcgcc gttccagcac
     9481 cctcctgtac gcccgccaga tcgacgcctt cgtcgagctc tggtgcgagg acgccagtta
     9541 cgaggccgcc ctgcccgtcg ccggcctgcc cgccgtcatc accggccacg aggcgctgca
     9601 cgcggccttc accggcctgg tctccgccgc gaagagcatc accgtgcacg acgtccgctt
     9661 ccaccagacc gacgaccccg acgtcgcgat catcgaggag cgcatggtcg gcgaactcgc
     9721 cgacggcgac cgctacgaga accgcctcat catccgggtc accttccggg acggccggat
     9781 cgcccacatg ctcgagtatt acggccagtt cgcccaccag gacctgctgc gcaagctcgg
     9841 cttcacggcc tagtggccca cgccgtagcg ggtggtgccc ctgctccagg cgtcgttgcc
     9901 gctgatccac gatcggacgt tcgccagata gccggcgagg tcgcgggcct gctcggggag
     9961 cccgtagtcg cgggggagcg cgagcgcctc ggcctcgaga tcaaggtact actcgatgag
    10021 ccggtcgtga gcgaacctgg tctgccaggt gggcgacatg cccatgcggc tgcgcgcaca
    10081 gatcggcgta ggcccgcacc agcggcggtg cgctgatcgg cagcgcgccc gcctcgccgt
    10141 aggcgaagcg gatcatcggg tcgaggaccg tccgggccag cgcgggatcc tcgccgagcg
    10201 ggccgtcgaa ccagtcgtcg aaggggaaga accagtacat ccagtctgac gcgagctggg
    10261 cgccgagacc gcgggcgtcg gggaacgcat aacccgtgag caggtcgaac ctcggttcgt
    10321 cgagcctgcg gatctcctgc tcgccggtga cgatcccgaa cgacgcggcc cactggaggc
    10381 tgcgccggcg catccgctcc acctcgggat tcagccgcgc cggccacggg atcctgaatc
    10441 ggggcccgcc gtcttcggca tcgggcggcg tcggatccgt gtcggggtgc gccgggcgcg
    10501 ccgtcgaggt ccccgggccg gtcggacaag ccgcttccag gtggcccgga accttcatac
    10561 acagctcctc gtgatgtcct gtgcgacgcg tcggctcagt tcggggcagt ctgcatcaac
    10621 acggaaccga tatgatcagt cactcaaagt agtgtttcct gggatgtggt gtctgccgga
    10681 tgccctcgaa ggcgaccatt gtgtgacctc cccggcttca gagcacgatg acaaggaacg
    10741 cggtggcggc cagcgcgagc gccaccaggc ccggcgtgcg ccggttgatc ctcgcctcct
    10801 gccaggagcc gcgcagcagg atcaccacga cggcgagcgc ggccaccagg tgcactcccc
    10861 accagaggct ctccgggccg tgacctccgg cgagtcggga gccgctgaac gcgatcagca
    10921 gcccgagcgc gaacaccacc gccgcctcgc ggtaccggcc gagcagcgcc catacgaccg
    10981 tgcaggccag gaacatgatc gaggcgctct ggtgcagccg ccgggagagc aggccgccct
    11041 cgatgtcgag gctgccacgc agagccgttt cgtattcgta gggctcgctc atgtgcacgc
    11101 cctggagggg aaggtaggag ccgtcgtaga agatctccgc gtcggcgggc acgaaatgga
    11161 tcatcagata gaccccggtg cccaggatca cgaggaagag gcagatcagc acctcgccgg
    11221 ccaactgccg tgcgcgttcc atggtgtgcg ttctccttca gctgttcagg agccggtcga
    11281 gactccggca ggcgcggccc agcgaggtcg aaccgcgttt cttcgccagg gcggccacct
    11341 cggggatctc gccccgggcg tcggcccacg tggcgacgtc gacggccagc acgacggcgt
    11401 cggtgtgcag agccagggcc cgctcgccct ccctcgggag gaactcggcc aggaagtggc
    11461 gcaggatcgt ccacacctcg gcgtgcgcgc cctgcctggc ggccagctcc agcgagggca
    11521 gcgcggggcc ggcctcgtag cgggtccggc ggcccagcat gcccagcagc ctgcctgtct
    11581 cggcagcggg gagagcgtcc ttggccgcga gctgaagcag cgggggcacc gcgtcgtcgc
    11641 cttcctcggt gaggaagtag gccaggacca gggcggtcgc cgggcccgac gcgccttcgg
    11701 cgagagccag gtcgtggagg tcgccgatcg cgatgtcggg cggatcgttg cggtcgatca
    11761 ggccgctcgc cagatgggcc gccgccagct cccggtggtg aggcgtcata ccgggccagt
    11821 agtgacgtga ctcgcgccgg taggggcgcg tctcgggcat ggtcagcagg gtgtcgaggg
    11881 gggcgatgcc gaggggttcc gccgctttcc agctcacgtc ggcgatgccg cgctggtcga
    11941 cgacgtgatc gtcatagggg acgacgcggg agccgagggc cagggtccag gtcagctcca
    12001 cctccggggg cgggggcgcg ccgcgctcga accatcgggc caccgctgcc gagccgctgt
    12061 ttctcgcgcg ttcggccagg tcggggccga gatcgggcgg caggcgcaag agtgcctgag
    12121 ccaggtcgag cggcaacggt tcgtggctcg attccgcgta cacggccagc cggtcgagaa
    12181 ggggcccggg gtcgaggtgg ccgtcggcgc gggtgggggt ggccagcagc accgggggca
    12241 gcactccggc cttcgccgcg cgcaggagtt cgtcgaggcg ggacagcagg aaggtgtgcg
    12301 ggcgggcgca ctcccccggg tcgggcaggc ggttccaggg ggcgggctca ggtcgcgtcg
    12361 ccgggtcgta gatgtcggcc gtccccagga cctcctcctg caagtagcca ggcaacgcgt
    12421 gcagcgggac gaccgtgcgg agaccgggga acagctggaa ggccgtggcc gcgacgctca
    12481 ccatgccgcc gtacagggac tgcccgagat cgcccgacag gccggggaag gcgtgctcgg
    12541 cgtcggtccg ctgctcctcg gagaccggtt tctggtgggc cgggcgccgg gccgcgggcg
    12601 gtcccggatc ttcgccggga ttgacgagct ccctggccag cgcggtgcac cagtcgcgcg
    12661 gcgacggcca ggtcagggcg tcgtagagca ccggccgcag gtgttcgccg aaccgccggg
    12721 ccagcgttct cctgaggggg ccgggattcg tgtgcgcggc ccgcacgacc gccgccagcc
    12781 acgcctcgac gtcgatccag ccgatctcct ccggcgggcc gctctccaag ccggagacgg
    12841 ggaacgggcc ggggacggtg acctcgggca gacgtccggg agtgaagtcg gcggtccagc
    12901 ctccggtgaa gcgctcgaag agctgccggg ccgaggccgg cggcaggagt tcggccgcct
    12961 ccaggatcgg ggcggggtcg gtgagatggc cggcgtgctt gagcgtgagg gccaccgccc
    13021 ggtcgcgcac ctcgggagag ccgtgcgcgt aagcgttggt cagcgcggtg acgtgcccgg
    13081 cgacgcgttc cggtgcggcg gccaggtcgc ggtcgagcca ggtcaggccc ttcacggcga
    13141 gcttggcctc cgggcggaag gtcatcgcct cgatcgcctc ggcgacgtcc gccggatcga
    13201 gccgcgcctg gtgcacctgc tgccaggcca gctcggccac cggccccggc gcggcgggca
    13261 gcagccgcag gtagtcgcgc agccgcccgg ccgactcctc cggcgtgggc gccacgagcg
    13321 tgtgcagccg gacgaagaac ctcaggtccg gtacgtcgcc accacgcagg aaacggccca
    13381 cgcagccgtc gatgatctgc tcgcggggta actcgcggac ggtcagggcc agccaccggg
    13441 acggcttagg ggtgagcgtc tcggcggcca gcgcccggcc cgcgccgtcg gcctcgaaga
    13501 ttttcgcgag cagctggggt gtgagcgggt ccttggccac ccccaccgcc gacaaccagg
    13561 ccaccaccag cggctcatgg tcgggcgggg tgaccacgcc gtcgcgcagc aacgccaggg
    13621 ccagtgacgc gatccggtcg tcgggacggc ggatgcggcg cgcgagccgt accgccagat
    13681 cggcccgcca ggccgccggg cgaccggaga ccgccttcac cagccggtgc gggctcggcg
    13741 cggtcgacca gcgggggttg aagtcgcgct tggtcagcca ggtgaccacg gcggccgggc
    13801 cggagatggt cgccgcgccc accatcatca gcacctcgga cgagccgatg acctggctgt
    13861 catcggtcca gtcgaagacg tccccgtcga gggagacggc cagcagcggg ctgtcgcgga
    13921 gctccgcgag caggcccggc agctcggcgg cgacggcggc ccgttcggcg tcggtgagct
    13981 ccggcaaccg caacgccagc ccttcggcgt cgcggttcgc cacgagattc ctgatctcgg
    14041 tacggatgtc gtcgacggtc atgacgccag cccttcgacg tcgcggctga gcgtgaagat
    14101 gtcgcgatat gtcatgacgt ggctccggtg agctgggtgt ggaggcgctt gcattcgtgg
    14161 acgaagcgga tcgtccggcg gctgcgcgcg aactcggcga cgatcgggat ctcgccgctc
    14221 gcgccggtcc agcgggccac gtcggcggcg aaggcgacga gttcggtgtg ggtcacggtg
    14281 atgcgccgtc cttcgcccgg cagcatcccg ggcaggaggg cgcgcaggat gcgccacacc
    14341 tcgtggtgac ctccggcctc ggccagctcg gtcagggcgg cgatggcggg acgggtctcc
    14401 agccacgtgc ggcgcagtgc cagtgcgagc tggcggccga tcgcctcggc gggcaggtcg
    14461 cctctggccg ccatccgcag gaccaccggg atcgtcccgg gcacgccgcc cgccagcagg
    14521 accgcgatgg tcaccgccgt cgcgtcgccg agggggccgt cggcggcggc caggcgggac
    14581 agctccgccg gggtcacgct cgcgtaccag cggctgcgca ggacgaacgg cagcatgttc
    14641 acggccgtca cctcgcggtg cgagggcagc atggccggcc acccggcgat cccgctgccc
    14701 atctcgtcga acgaaccgtc tggcggctgc ctcagcagcg ccccgtcgat cagctcgtca
    14761 ccggtgggcg tggcccgcag caccggggtc aggcggacct cggtgaagtg ttcgggttct
    14821 ccgtcgccga acgggaccat ggacgcgttc accatgtggg tccactggaa accgcactcc
    14881 gggtcgggca tacccggccc ggccagccat tcgtgtgccc tgcgcgcagc aaccgaatcg
    14941 atcttcgcgg cccggtcggc ggcggccggg tggccgcccc ggggcagccg tagcagcgcc
    15001 tgggtgaggt cggccctcag cggttccacg cctgcggcgg cgcacgtctc caggcggtcg
    15061 accagcacgt cggggtcgag gtggccggtc atccacgtcg gcgtggccag cagcaccggc
    15121 ggcagcgtgc cggtgcgcag agccccgtac agctccgcgt agcgggtgac gaggaagtcg
    15181 gacggcggcg agagatgttc cgggtgcggg agccggtgcc gtcgccgcca ctcgtgcagg
    15241 ggcggaagct cccggcgctg gaaggagcgc aaccgggacg ggacgaaggc gacggtgacc
    15301 gcgaccagcg gctcgtccgg gccgggcggg ggcaccggca ggcccttgcg catggcggtg
    15361 atccggtcgg ggttcacccc cagctcggcc aggcgctcgt acacgccacg cagggagtcc
    15421 tgcggcatgg cctcgtccga gtcgtcgtag aggactccgg gctccaccgg acgggacgca
    15481 cgccgatccg ggacggcgtc gtcgaagtcc tcttcgaagt caccgtcgta ctcgtcgtct
    15541 tcgtcgggag cggggtccgg ggtccggtcg tcaccggagg gttgtccgta cagatagatc
    15601 cggccgttgg cgtaggggat cggctggccg gcctcgtcgg ccacggcccc cgccgggccg
    15661 tcgtcgctgg tccaggtgat gaagacgacg cggagcgggg tgtcgtcgtc gaggtagcgg
    15721 ccgggtcgcc agccgctacc ggccaccgcg aaccgccgtt tcggcgggtt cagcccgatc
    15781 tcctcgcccc ggttgaccaa gcgggccgag accgagtgac tcgcgctgtc ccagaacttc
    15841 tccgggccgc gcggcgggat ctccggcagg gcgccgggcg tgacgagctc ctcggccagc
    15901 acggtctgcc attcgtcggg gtcggtgatc tgatcccggt tctgccggtg ggtctgctgc
    15961 tcgacgtgcg gcttcagcct gcggcgcagg ttcgtgcggt tctccgcgac cccgccgacg
    16021 aaggccgcca tccagcgttc ccatccgatc caccgctcgt ggtagttcgg gaagatcgac
    16081 ggcttgggga aactctccct ggcgggcacc tcgggcagcg gagagaactc cttgcgctcc
    16141 tgcggatcct cggccggggc ctcgccgccg aagcgttcgg ccagcctcgc gcccaggtcg
    16201 gcggggaggt gccgggcgcc gtcgaggatc gcctcggcgt ggtcggcgaa caggccggcg
    16261 tgcttcaggg tcagctcggt cgagcggttg cgcacgtcga aagacgcgtg tgtgtacgcc
    16321 gtggtcagcg cgcccacgaa gtcggcggcc acctcgggag tggcccggat cgtctggtcg
    16381 agccagcgca gaccccgggc ggcgaatttg gcctcggccc ggaacgtgag cgcctcgacc
    16441 gcctcgacca gatcggcgtg gtcgagcggc atcgccgcgc gcacctgtcc gacggccagt
    16501 tcggcgaccg ggcccggagc cgacggcagc aaccgcagat agtcacgcag ccggggagcg
    16561 gactcctcgg gcgtgggatc gaggagcgtg tgcagccgga cgaagaaccg caggtcctga
    16621 acgtcgccac cacgcaggaa cctgctcacg cacccgtcga gcgcctgctc cctgggcagc
    16681 tcgcgagcgg cggcggccag ccagcgggtc ggcccggggt ccagccgctc ctcgcggagg
    16741 gcacggcccg cgccgtcggc cgtgaagacg tgcggcaaca gaacagaggt caacgggtcg
    16801 ccggccaggc tcggcacggt cagccaggcc gccaccagcg ggtcgtggtc gggcggcgtc
    16861 gccccggact cacgcagcag cgccacggcc agcggcgcga ggcggtcggc gggacggcgc
    16921 acccgctggg ccagccggac cgcgacatca cgacgccact ccggcggacg ggtcgccgcc
    16981 acccggcaca cctgggccac gtcgacggtc ccgccccagc gccggttgac gtcgcggccg
    17041 gtcatccacg tcacggcggc ggcggggccg gtgatgacgc cgacaccggt cagaagcagg
    17101 gccccggccg aggtgtcgag ctgctcaccg acgagctcat ccacgtcgtc ggggtcatca
    17161 gcggggggac gccgcaactc cttcaggagg ccgggcaact gcccgcccag ctcggcgcgc
    17221 tcctcctcgg tgagcgtgac cagccggtcg acgaggggag tgacccggtt gccggtgacc
    17281 aggtcgcgga tctccgtcca gagggtcatc gggagacctc cacggcggcg cgggcgacga
    17341 tccgggcggc cagcacgtgt ttgcacggcc cgcgcgagcc gcgatggtcc caccaccact
    17401 cgcaggtgca ggagccgtcc ggcagcgaga cccggcgggc gccacccgag gtgtggacgg
    17461 tggccagctc gccctcaagg cgcacggccc ccgcctcgac cagcttgcgc gccttggcca
    17521 gccgggggtt gagcaggatc acgttgtcct tgtcgtaggg cagctcacgg tggaagtggg
    17581 cggcctccgc caggtcgtag ccgacccggc cggcgacgcc gagctgcgtc agcgcggccc
    17641 ggacccggtc ggcggggatg cccgcacggt cggccagcag gccgatctcg acctcgggct
    17701 cgaagttgag cagcatgccc accacgtcgg cgtcgttctc tgcctcgtcg gtggccaggt
    17761 cgccgagcac cgcgccctca ccggagaagc cgcgccagcg ctcgggggag agggtgagcg
    17821 tgtagcgcat gcccggcagt tcgagctccc aggcgctgga ggcggcgtcg gcagggccgt
    17881 acacacgaag ggttttcgcg aagcgcagca gtggcaggag ggtgccgagc cggtggggac
    17941 cggcgagctg gaccgcaccc gggacctgac ggtcggcgac cttcaggtcg cgacccgact
    18001 gcgtcaccca caccgtgccc ttcttgggca gcccgcgcag gaaccgcacc gccgtcatgc
    18061 cggccagctc gccgcgctgg tccatgcccg aggcgatgac ctgcacctcg gcgaatcccc
    18121 gtaaccagcg ctcggggagc ggcaccttct tctccacgac agcgccgtcc atcgtggtca
    18181 cgaccagctc gtcgagcccg acccccaggt gcagcgggtc acgcgcgccg atgcgcgcca
    18241 gcgcctcccg gagcggcgcg ttcacgtcga cgttggtggt gccgcgatcg aagacctcgc
    18301 cgtcgagggc gccgcccagc aggtcgagcc gggcgtagac cccgccgcag gccgagaacg
    18361 actcgaaccg caaccggtct ccgttgcacg tcaccaccgg gtcgcgcacc cagccgggcc
    18421 gcgcctgcgg ctggtggtag cgggccagcg ccacgtcggc gacgccgagc agcgcggccg
    18481 cggcgggcgc cgcctgggtc accacgccgc tgaagaaccg gggagccagt gccgggccgg
    18541 acagcgcccg gccgccggag gtcgccaatc cgagacgacc gtcgacaagg gaggagggcg
    18601 cagcgtaggt gtacgcctgc gtcgctgaag tcatggccgg gacaatagat cttcccaccg
    18661 acaaaccggt gcggcccgcg agaactagat cggaaaactg ccgcgacgcc agtgccagcg
    18721 gcggccggcc cgcagatggt cgacgatcgc acgctggagc acgttgtcgc gcggcagccg
    18781 atcgagcggt gtggtcaggg tccggaacac gaaccgcagc acctcgacgt cggcgtcggg
    18841 cccttccgac tcctcgtagg gctccagctc gaacctccgc tggaaccgga cgatgttgga
    18901 gtcttccggc agatactcct gtagttgcgg gtcgagcagc cacgagccgc agccgaaggc
    18961 ggtgtagcgc tcgtcgggga agtggcgcgg gaagaacacg cgggcctggt cgagcgaggc
    19021 gtcgatcgcc tcaggagtca tcgggcccga ctcggggatg tgcaggtcga gggtggtgcc
    19081 gccgcggtgg aactgcaacc ggcccatctc gtagacggcg ccgcgcgcgt gcagcgtcag
    19141 ccaggcttgc atgaccggcc agccctcgcg gttcatccgc cggtcgaccg ccaggttgcg
    19201 gccgaggtcc gccaggctca gccacgacac ggcttcgtcg acgccgtggt cgcggtggta
    19261 tgccatggcg acgtcggcca gggccagaaa ggcgtacaca tagagatgcc gccaggccgg
    19321 gccccgatcg cggggcagct caggaccggg caagagccag ccgccgcccc cgaggtcggc
    19381 gcggaccagg gcgatcgagc ggtcgagcag ccagcgcagc tccggggtcc agagcggaga
    19441 gccggggtcg ggccagccct ccatgatctc ggcggcgtcg tcgggccgca ccgcgagccg
    19501 gtcgagcatc gcgggcgcgt cggctttggc gggcagcgga gccgacggca ggtcgccggc
    19561 cagtcggtgc acgcgatcga catcctcgac ggatacgccg agccgggccg cggtgtcgtc
    19621 caagtccacg cggacgaccc tactcttcgg cctccggttt tccagggcct cacaccgcgc
    19681 tgaccgcgat caaagagccc aaggccatca gggtgccgac gatgccgagg gtcgcggtgt
    19741 catgatcgcg ggtggccgcg atccagctgg cgtgggcgcc catccagacg gtgccgccgg
    19801 agaccaccag caccacgatc cgcagtaggg agtcgtccgc ctcggctacc ggaacacccg
    19861 gctcgaatac cccgacacct ccgtgccgct gcgccggaat ctggcggtca ccccggtccc
    19921 gaactcctgc atcctgcgcg acctgatcga cgagatccgc cgcgagctgt ggcggtctcc
    19981 cacctggatg ccggcgtgct gatccgggtc acgctgacct tgtcgctgct ggccgccggg
    20041 tgtaccggcc cgcctcagga cgacctgtcc ggcccgccgt tcaccctgcg cgtcctggcg
    20101 ggtgacggtc tcgccgacat ggcccccatc ctgcgggacg cgatcccggc caccggcgtc
    20161 acggtcgcgc tgaccaccac cgacgacccg gccaggttcg agacggcagg gcgtgacttc
    20221 gacgccatct ggcccgcgtc cgaccaccgc ctgcggttgc gcgggggcac agcgcggctc
    20281 gacggcaccg tcgagatcat gtcgtcgccc gtgatcgtgg gcgtgagcac gcgggccgcg
    20341 caccgcctcg gctgggaccg ccgccccgcc acgtgggccg acatcgcggc ggcggccgcc
    20401 tcgggccgct tcaccttcgg catggccgat ccggcccgat ccgacgacgg cctcaacgcg
    20461 ctcgtggccg ccgcgaccgc cctgaccggg cccggcgcgc tccagcccgg cgaggagcac
    20521 cgggccgcgc cccggctcgc gggtctgttc acggggca
//
