LOCUS       NZ_BBXD01000015        12736 bp    DNA     linear   CON 08-AUG-2024
DEFINITION  Herbidospora mongoliensis strain NBRC 105882, whole genome shotgun
            sequence.
ACCESSION   NZ_BBXD01000015
VERSION     NZ_BBXD01000015.1
KEYWORDS    WGS; HIGH_QUALITY_DRAFT; RefSeq.
SOURCE      Herbidospora mongoliensis
  ORGANISM  Herbidospora mongoliensis
            Bacteria; Actinomycetota; Actinomycetes; Streptosporangiales;
            Streptosporangiaceae; Herbidospora.
REFERENCE   1
  AUTHORS   Komaki,H., Ichikawa,N., Oguchi,A., Hamada,M., Tamura,T. and
            Fujita,N.
  TITLE     Genome-based analysis of non-ribosomal peptide synthetase and type-I
            polyketide synthase gene clusters in all type strains of the genus
            Herbidospora
  JOURNAL   BMC Res Notes 8, 548 (2015)
   PUBMED   26452464
  REMARK    DOI:10.1186/s13104-015-1526-9 Publication Status: Online-Only
REFERENCE   2  (bases 1 to 413554)
  AUTHORS   Hosoyama,A., Oguchi,A., Ichikawa,N., Kimura,A., Ohshita,R. and
            Fujita,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAY-2015) Contact:Director-General Department Of
            Biotechnology National Institute of Technology and Evaluation,
            Biological Resource Center (NBRC); 2-49-10 Nishihara, Shibuya-ku,
            Tokyo 151-0066, Japan URL :http://www.nite.go.jp/nbrc/index.html
COMMENT     ##Genome-Assembly-Data-START##
            Finishing Goal                    :: Noncontiguous Finished
            Current Finishing Status          :: High-Quality Draft
            Assembly Method                   :: newbler v. 2.6
            Genome Coverage                   :: 62x
            Sequencing Technology             :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_001570545.1-RS_2024_08_08
            Annotation Date                   :: 08/08/2024 02:40:32
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,280
            CDSs (total)                      :: 8,202
            Genes (coding)                    :: 8,113
            CDSs (with protein)               :: 8,113
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 6, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 4, 5 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 21 of 89
            Pseudo Genes (incomplete)         :: 71 of 89
            Pseudo Genes (internal stop)      :: 4 of 89
            Pseudo Genes (multiple problems)  :: 7 of 89
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 18:23:16
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 58485
            Orig. end                         :: 71221
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            BBXD01000015.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..12736
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="hydrogen-cyanide"
                     /region_number="1"
                     /rules="(Fer2_4 and cds(Pyr_redox_2 and Fer2_BFD) and DAO)"
                     /tool="antismash"
     cand_cluster    1..12736
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(Fer2_4 and cds(Pyr_redox_2 and Fer2_BFD)
                     and DAO)"
                     /kind="single"
                     /product="hydrogen-cyanide"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..12736
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:7736](+)"
                     /cutoff="5000"
                     /detection_rule="(Fer2_4 and cds(Pyr_redox_2 and Fer2_BFD)
                     and DAO)"
                     /neighbourhood="5000"
                     /product="hydrogen-cyanide"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..7736
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="5000"
                     /detection_rule="(Fer2_4 and cds(Pyr_redox_2 and Fer2_BFD)
                     and DAO)"
                     /neighbourhood="5000"
                     /product="hydrogen-cyanide"
                     /protocluster_number="1"
     gene            49..2652
                     /locus_tag="AW272_RS21505"
     CDS             49..2652
                     /GO_function="GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA]"
                     /GO_function="GO:0008270 - zinc ion binding [Evidence IEA]"
                     /GO_process="GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020541009.1"
                     /locus_tag="AW272_RS21505"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="WP_066368101.1"
                     /transl_table=11
                     /translation="MRAPIALLSVAVAATTLLVPTGSAYAEPTPPPGVTTRDFGNGPER
                     NEEAAGLSEQTAGTLRALTAETPAAATERLKQFPFFRDFRDNERGYPRQNRLPVPAENA
                     ADASVKLGLTPYHAIAGKLNALQARSSRVSVEIIAKTHQGRDLYLVTVTAPESKSQTRE
                     QDAIRDRIENDSAKAAKDRKLPGKYKAPVFVNNNIHGNEWEGTDAALRVIEELATSNDK
                     KIVDLLNRTRLYFNVTANPDGRFGNTRANGAGFDMNRDFITASQPEVQAMRQVMIDTQP
                     VAMIDLHGYVNGTLIEPTTPPHGANYEYDLFIKNTYANGLGMEQAVLGLGYTPEKDGVN
                     PPTIPFRDDAEGWDDWPPIFTPQYAPFQGAVASHTIEFPMRVNNADYNNLPVEELRRRS
                     AINTDIAAAVMRATYTFVQDRHDQLIADQIETFRRGAAGEASKQVPLDIVPGFGPEDVY
                     NTTFPRAYVIPAGDKQRSVTAAARLVDHLIANDVRVEKAKRAFHANGKFYAEGSYIVDM
                     HQPKRGIANVMLEPGADITPRVDSMYDISGWSHALLWGATVDSISSLKVDSRQVDKADL
                     DGSVPWTNRSLALRVTDGREIQAVNDLLGQGVQVNWADNGTAVIPASARRAAEGVARTY
                     DVTFTQASSSAGTKLGQVRVAAAVSADELFTLRELGFQVTPVSTAVLNAGFDWSATDVL
                     FVSSGLNYGQLNAEAKAGLDAYLTTGGVVTRGSTGSTFNGAAGLLTLTPVAGRSDANGV
                     VKVTSTGGPVGGDAPAHSFVYSPRWFTGLGQEVRVEQAYATDGPLVSGHWRANTNGAGG
                     QDAARGQAAVVSGRDERGAAVVMFGTEPMFRNHPKGLFAQVAKALYWSSSAADVK"
     misc_feature    226..228
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            2764..3195
                     /locus_tag="AW272_RS21510"
     CDS             2764..3195
                     /GO_function="GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /GO_function="GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /GO_process="GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF012762.4"
                     /locus_tag="AW272_RS21510"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="cellulose binding domain-containing protein"
                     /protein_id="WP_066368103.1"
                     /transl_table=11
                     /translation="MKKNSLTAFAVLALVAGIALVVWRVTGVSAEPVHFHPSPSPVAAT
                     AKCTADVKLITTWPGGFQGEVKITNTGTTPINDWYVQWNVPLEAKVTQSWNGTWMQSGP
                     SVMIHAEKWNKVLAPGATETAGFKGDAKDAPTIADIYCG"
     gene            complement(3237..3980)
                     /locus_tag="AW272_RS21515"
     CDS             complement(3237..3980)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017543684.1"
                     /locus_tag="AW272_RS21515"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="vancomycin high temperature exclusion protein"
                     /protein_id="WP_066368105.1"
                     /transl_table=11
                     /translation="MKRAVFLGAVAASLLALAPMTWAWLSSEGHRIDTNHPGWTAEVPV
                     APVALVLGAGVNGDWPSAMLRDRLDIGVELYRAGRVKALLLSGTNHIKEYDEPTVMRDY
                     LLARGVPAAALVLDYAGLDTWDSCVRAKKIFGAVQVTVVTQTFHLPRAVTLCREAGLTT
                     FGVGDDSQRKYALTTYTYAAREFFATAKGFLDAVVLKSEPRFLGPYETSLDTALAASHV
                     RGPIPDAMDRKMAPLESVVEETTRS"
     gene            complement(3987..4928)
                     /locus_tag="AW272_RS21520"
     CDS             complement(3987..4928)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013132222.1"
                     /locus_tag="AW272_RS21520"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="heme A synthase"
                     /protein_id="WP_245657656.1"
                     /transl_table=11
                     /translation="MKRLLSSVWSPSARNMRAWALAAVVVNAGITVTGAAVRLTGSGLG
                     CPTWPRCTADSFVPVAHPDHSPINTAIEFGNRMFSLMVLAVAIAVFLAAWKLKRRSLIP
                     LALVQPLGVIFQALWGGLVVRTALNPVTVSVHFLVSIGMIAAAYMLFARSTEGDGPVVP
                     TVHPWIVTLGRVLTGAMLVLLVAGVVTTGTGPHSGDERASRFDFDIEVVARIHADIVWV
                     VIGLTFALLFALYLTKAPEQAKRAALILFGVELAQGVIGYVQYFLAVPALLVGFHVLGA
                     ALVWIATLRVVHMLRVRTEITEVVNHDAVARV"
     misc_feature    complement(4982..4998)
                     /note="TFBS match to ZuR_variant_2, Zinc-responsive
                     repressor, confidence: weak, score: 17.56"
                     /tool="antismash"
     misc_feature    complement(4984..4994)
                     /note="TFBS match to HypR, L-hydroxyproline utilization
                     repressor, confidence: weak, score: 20.07"
                     /tool="antismash"
     gene            5001..6164
                     /locus_tag="AW272_RS21525"
     CDS             5001..6164
                     /EC_number="1.-.-.-"
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     hydrogen-cyanide: DAO"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1103: FAD dependent oxidoreductase"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020547415.1"
                     /locus_tag="AW272_RS21525"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="FAD-binding oxidoreductase"
                     /protein_id="WP_245657657.1"
                     /sec_met_domain="DAO (E-value: 1.7e-63, bitscore: 209.5,
                     seeds: 521, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MDVLVVGAGIVGASCAYFLTRAGLRVTVVDRGGVASGTTGAGEGN
                     ILVSDKEPGPELDLALRSTQLWRELAEECGGFEWDPKGGLVVAETQEVLDALVIFAERQ
                     QQHGRIAGSEVGTPEGAAPAPADVVATCIAAGDLRELEPHIADGLAGGVHYPQDAQVQP
                     MLAAARLLKASGARLVRTAVERFLVSGDRVIGVRTGEGDVYADAVVNAAGTWAGSFAQA
                     PLPIRPRKGFILVTQPFVVPPIRHKVYTAAYITNVASDAEGLETSGVIESTQAGTVLIG
                     ASRQRVGYDTSIDYRVLAKLARQAIGLFPVLADRQVMRAYAGLRPYSPDHLPVIGPDWR
                     LENLFHACGHEGAGIGLAPATAEMITAQITGEPTPRDPAPFAPGRFA"
     gene            6161..6397
                     /locus_tag="AW272_RS21530"
     CDS             6161..6397
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     hydrogen-cyanide: Fer2_4"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017623950.1"
                     /locus_tag="AW272_RS21530"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="WP_066368117.1"
                     /sec_met_domain="Fer2_4 (E-value: 7e-24, bitscore: 78.0,
                     seeds: 114, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIEFGFQGRTITAEEGQSIGAALHAAGIRSWRTTRFGNRPRGLFC
                     AIGACFDCLLTVNGTPDQRACQTVARSGDEVSP"
     gene            6492..7736
                     /locus_tag="AW272_RS21535"
     CDS             6492..7736
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     hydrogen-cyanide: Pyr_redox_2"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     hydrogen-cyanide: Fer2_BFD"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1176: alkyl hydroperoxide reductase subunit"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /locus_tag="AW272_RS21535"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="WP_066368119.1"
                     /sec_met_domain="Pyr_redox_2 (E-value: 6.3e-24, bitscore:
                     78.9, seeds: 64, tool: rule-based-clusters)"
                     /sec_met_domain="Fer2_BFD (E-value: 7.8e-09, bitscore:
                     30.1, seeds: 211, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIYDLAVIGAGPAGLTAARVAARRGLSVALVDAYARTGGQYLRGA
                     TQPRLTGIDVKLGQRVWAVEKSGEGFQIHASTTTTASTLLVATGTHDKVIPFPGWDLPG
                     VLTAGGAQALVKGERVKPGEEIVVAGTGPFLLPVAVTLLDAGVRVKEVLEANRPSPHMA
                     AALARVPSKAREAVAFARRLADARVPYRTGHAVVAAHGKDSVEGVTVAKITKDWKPYAW
                     RTVDCDTLAVGYGFTPQVDLLVQLGCRTEGDHVVTDDRQRTSVDGVLAAGEVAGVAGAQ
                     AAVVEGALAVHALIGEPPPRLLAEKRRLRRFARALELSYPVRDGWQHWLRPDTLVCRCE
                     EVTVTAIEEARDLGADDPRMVKLLARPGMGWCQGRMCGHAVARLTGHEPAPQKRPFAQP
                     ITLADLTLHDREDST"
     gene            7733..8602
                     /locus_tag="AW272_RS21540"
     CDS             7733..8602
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012892474.1"
                     /locus_tag="AW272_RS21540"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="WP_066368121.1"
                     /transl_table=11
                     /translation="MIRPWHGIMVATALPFTGDLTVDHDAYAAHCRRLVDEGCDGVVPN
                     GSLGEYQTLTPDERARVVRTAVEAVGGDRVMPGVAAYGSREALTWAEQARDAGCASVML
                     LPPNAYRADENAVLEHYRTVAKAGLPIVAYNNPIDTKVDLTPALLAKLFGEGLIVAVKE
                     FSGDVRRAYEIREVAPDLDLLVGSDDVVLELLTAGAKGWIAGFPNSVTRQCVDLYRAAA
                     AGDLNTAVPLYRDLHPLLRWDARVEFVQAIKLSMDVAGLNGGACRPPRLPLPPATAAEI
                     RAATEKVM"
     gene            8604..9602
                     /locus_tag="AW272_RS21545"
     CDS             8604..9602
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019891275.1"
                     /locus_tag="AW272_RS21545"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="proline racemase family protein"
                     /protein_id="WP_066368123.1"
                     /transl_table=11
                     /translation="MRTRRIFHAVDSHTEGMPTRVITGGVGVIPGATMAERREHFMAHL
                     DHLRTLLMYEPRGHAAMSGAILQPPTRPDADWGVLYIEVSGCLPMCGHGTIGVATVLVE
                     TGMVEVVEPVTTIRLDTPAGLVTASVAVRDGVAESVTIENVPSFAVDLQKTIKVDGFGE
                     LTYDLAYGGNFYAILPIESVGLPFDRAYKQQILDAGLAIMDVINENEPVHPEDSGIRGC
                     HHVQFLAPGSTARHSRHAMAIYPGWFDRSPCGTGTSARMAQLHALGELPIDAEFVNESF
                     IGTRFTGRLVATATVEDRQAVVPHITGRAWLTGTAQYFLDPGDPFPQGFLL"
     gene            9669..10343
                     /locus_tag="AW272_RS21550"
     CDS             9669..10343
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1071: GntR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020544794.1"
                     /locus_tag="AW272_RS21550"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="GntR family transcriptional regulator"
                     /protein_id="WP_066368125.1"
                     /transl_table=11
                     /translation="MATAEGLGLPVVGERQSLREQVAQALRDALVAGEMRPGVVYSAPV
                     LAAQFGVSATPVREAMLDLAKEGLVEAVRNKGFRVTEMSDTDLDHITEIRRLIEVPTVA
                     KLAGSGLSADFERLRPLAREIVTAAERGDLLTYVNADLRFHVELLGLNGNAHLVEVVRE
                     LRSRARLYGLKSLADAGKLVDSAREHVELLDALIAGDSRAAEHVMKHHIQHVRGIWANK
                     PE"
     gene            complement(10345..11217)
                     /locus_tag="AW272_RS21555"
     CDS             complement(10345..11217)
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1166: transporter,
                     EamA family"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020544795.1"
                     /locus_tag="AW272_RS21555"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="DMT family transporter"
                     /protein_id="WP_245657658.1"
                     /transl_table=11
                     /translation="MTADTEVSKPWTRAAGAVPPPALVMLGIVSVQIGAGLAKDLFAQL
                     SPTAVVFLRIATGTLVLVAFVRPRLRGLSWQSVRIGLAFGVILAVMNLTFYEALARLPI
                     GIAVAIEFMGPLGVAVAASRRKLDVVWVVLAGTGIVLLAPWGNAANADWVGIGFAAAAG
                     VCWGAYILLSASVGQRFPGTSGLVFAMIIATVVVAPPGIASGGLDLLDPTILLIGLGVG
                     LLSSVIPYSLELEALRRMPKRVFGILMSLEPAVAAMVGLVVLGEVLHLREWAAIGCVVV
                     ASIGAARSG"
     gene            complement(11314..12078)
                     /locus_tag="AW272_RS21560"
     CDS             complement(11314..12078)
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1065: ABC-2 type
                     transporter"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012892461.1"
                     /locus_tag="AW272_RS21560"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="ABC transporter permease"
                     /protein_id="WP_066368130.1"
                     /transl_table=11
                     /translation="MTTLDLSPAPGAAPLARMVLAQAGAEVRATLRNGEQLLLTLIIPI
                     LLLVGFSKAPFLDVAGPKVDFVAPGVIALAVLSTAFTGQAIATGFERRYGVLKRLGATP
                     LSRTGLMLAKTTAVIVVELLQIAVLVLVALALGWSPHGNPLTVLLLVILGTAAFSGLGL
                     LMAGTLRAEATLAGANLVYLLLLALGGVVLPITAFPEGIRPLLEILPITALTDGLRSTL
                     TGVGGFPIGAALVLLSWAVLALAAAARWFRWE"
     misc_feature    12104..12118
                     /note="TFBS match to IolR, Regulator of the inositol
                     catabolism, confidence: medium, score: 18.26"
                     /tool="antismash"
ORIGIN
        1 atttgatctt aggaatgggg cacgcaccct gatcacggag gttctctctt gcgtgccccc
       61 atcgccctcc tgagcgtcgc cgtcgcggcc accacgctcc tcgttccgac gggttcggcc
      121 tacgccgagc cgaccccgcc gcccggggtg acgacccgcg acttcggcaa cggtcccgag
      181 cgaaacgagg aggcggccgg cctctccgag cagaccgccg gcaccttacg agcgctgacc
      241 gccgagaccc ccgcagccgc gaccgagcgg ctgaagcagt tccccttctt ccgggacttc
      301 cgggacaacg agcgcggcta cccccggcag aaccgcctgc cggtgccggc ggagaacgcg
      361 gccgacgcct ccgtcaaact cggcctcacc ccctaccacg ccatcgcggg caagctgaac
      421 gccctccagg cccgcagcag ccgcgtcagc gtcgagatca tcgccaagac ccaccagggc
      481 cgtgacctct acctggtgac cgtcaccgcg ccggagagca agagccagac gcgtgagcag
      541 gacgccatcc gcgaccgcat cgagaacgac tccgcgaagg ccgcgaagga ccggaagctg
      601 cccgggaagt acaaggcgcc ggtgttcgtc aacaacaaca tccacggcaa cgagtgggag
      661 ggcaccgacg ccgccctgcg cgtgatcgag gagctggcca ccagcaacga caagaagatc
      721 gtcgacctgc tgaacaggac ccggctctac ttcaacgtca ccgccaaccc cgacggccgg
      781 ttcggcaaca cccgcgccaa cggcgccggc ttcgacatga accgcgactt catcaccgcc
      841 tcgcagcccg aggtccaggc gatgcgccag gtcatgatcg acacccagcc ggtcgccatg
      901 atcgacctgc acggctacgt caacggcacc ctcatcgagc cgacgacccc gccgcacggc
      961 gccaactacg agtacgacct gttcatcaag aacacctacg ccaacggtct cggcatggag
     1021 caggccgtgc tcggcctcgg ctacacgccc gagaaggacg gcgtgaaccc gccgaccatc
     1081 ccgttccgcg acgacgccga gggctgggac gactggcccc cgatcttcac cccgcagtac
     1141 gcccccttcc agggcgcggt ggcctcgcac accatcgagt tcccgatgcg ggtcaacaac
     1201 gccgactaca acaacctccc ggtcgaggag ctgcggcggc gttccgcgat caacaccgac
     1261 atcgcggcgg cggtgatgcg ggccacgtac accttcgtgc aggaccgcca cgaccagctc
     1321 atcgccgacc agatcgagac gttccgccgg ggcgccgcag gtgaggcctc caagcaggtg
     1381 ccgctggaca tcgtgcccgg cttcggtccc gaggacgtct acaacaccac cttcccgcgc
     1441 gcctacgtga tcccggcggg tgacaagcag cgttcggtca ccgccgccgc gcgcctggtc
     1501 gaccacctga tcgccaacga cgtgcgggtg gagaaggcca agcgggcctt ccacgccaac
     1561 gggaagttct acgccgaggg ctcctacatc gtcgacatgc accagcccaa gcgcggcatc
     1621 gcgaacgtga tgctggagcc gggcgccgac atcaccccgc gcgtcgactc catgtacgac
     1681 atctccggct ggagccacgc cctgctgtgg ggcgccacgg tcgattcgat cagctcgctg
     1741 aaggtggact cccgccaggt cgacaaggcc gatctcgacg gctcggtgcc gtggacgaac
     1801 cgctcgctgg ccctgcgggt caccgacggc cgggagatcc aggccgtgaa cgacctgctc
     1861 ggccagggtg tgcaggtcaa ctgggcggac aacggcaccg ccgtgatccc cgcctcggcc
     1921 cgccgcgccg ccgagggcgt cgcccgcacg tacgacgtca ccttcactca ggcttcgtcg
     1981 agtgccggca ccaagctcgg ccaggtccgg gtcgccgccg cggtctcggc cgacgagctg
     2041 ttcaccctgc gtgagctggg cttccaggtc accccggtgt cgacggccgt gctcaacgcc
     2101 ggtttcgact ggagcgcgac cgacgtgctc ttcgtctcca gcggcctgaa ctacggccag
     2161 ctcaacgccg aggcgaaggc cgggctggac gcctacctga cgacgggcgg cgtggtcacc
     2221 cgaggcagca ccggctcgac cttcaacggc gccgccggcc tgctcacgct gaccccggtc
     2281 gccgggcgca gcgacgccaa cggcgtggtg aaggtcacct ccacgggcgg tccggtcggc
     2341 ggcgacgccc cggcccactc gttcgtctac tcgccccgct ggttcaccgg cctcggccag
     2401 gaggtccgcg tcgagcaggc ctacgcgacc gacggtccgc tggtcagcgg ccactggcgg
     2461 gcgaacacca acggcgcggg cggccaggac gccgcccggg gccaggcagc cgtggtcagc
     2521 ggccgagacg agcgcggcgc ggccgtggtc atgttcggca ccgagccgat gttccgcaac
     2581 cacccgaagg gcctgttcgc ccaggtggcc aaggcgctct actggtcgtc ctccgcagcg
     2641 gacgtgaagt gaccacctga aatcccacgg cccctggcgc tccggccggg ggccgtgttt
     2701 caattccggc gggcccgact cccacgtgac atgtgttgat ctgtcatgtc tggaggcccc
     2761 ccgttgaaga agaactccct gaccgccttc gccgtcctcg ccctcgtcgc cgggatcgcc
     2821 ctggtcgtct ggcgagtcac cggggtgagc gccgagccgg tccacttcca cccctcgccg
     2881 tcacccgtgg ccgccacggc gaagtgcacc gccgacgtca agctgatcac cacctggcct
     2941 ggcggtttcc agggcgaggt gaagatcacc aacacgggga ccacgccgat caacgactgg
     3001 tacgtccagt ggaacgtccc gctggaggcg aaggtcaccc agtcgtggaa cggcacctgg
     3061 atgcagagcg ggccctcggt gatgatccac gccgagaagt ggaacaaggt cctggcgccc
     3121 ggcgcgaccg agacggccgg cttcaagggg gacgccaagg acgccccgac gatcgccgac
     3181 atctactgtg gctgaccctg gggctgaaag attcacggga atccccagct cagccgctat
     3241 gagcgggtcg tctcctcgac gacggactcc agcggtgcca tctttcggtc catcgcgtcg
     3301 ggaatcgggc cccgcacgtg ggaggctgcc agcgccgtgt cgagcgaggt ctcgtacggc
     3361 cccaggaacc tcggttccga cttgagcacg accgcgtcga ggaagccctt ggcggtcgcg
     3421 aagaactccc tggccgcata ggtgtacgtc gtcagcgcgt acttccgctg ggagtcgtcg
     3481 ccgacgccga aggtggtcag cccggcctcc cggcagagcg tcaccgcgcg gggcaggtgg
     3541 aacgtctggg tgaccacggt cacctggacg gccccgaaga tcttcttggc ccgtacacac
     3601 gagtcccagg tgtccaggcc cgcgtagtcg agcacgagcg ccgccgcagg aacgcccctg
     3661 gccagcaggt agtcgcgcat gaccgtgggc tcgtcgtact ccttgatgtg gttggtgccc
     3721 gacagcagca gcgccttcac ccgcccggcc cggtagagct cgacgccgat gtcgaggcgg
     3781 tcgcgcagca tcgcggacgg ccagtcgccg ttcacccccg cgcccagcac cagcgcgacg
     3841 ggcgccaccg ggacctcggc ggtccagccg gggtggttcg tgtcgatccg gtggccctca
     3901 ctggagagcc acgcccacgt catgggcgcc agcgcgagca gactggccgc gacggcgccc
     3961 aggaaaacgg cacgtttcac gaagggtcag acgcgggcga ccgcgtcgtg gttcacgact
     4021 tccgtgatct cggtgcgtac ccgcagcatg tggacgaccc gcagcgtggc gatccacacc
     4081 agggccgccc ccagcacgtg gaacccgacc agcagcgccg ggacggcgag gaagtactgg
     4141 acgtagccga tgaccccctg cgcgagctcc acgccgaaca ggatcagcgc ggcccgcttg
     4201 gcctgctcgg gcgctttggt gaggtagagc gcgaacagca gcgcgaacgt cagcccgatg
     4261 acgacccaca cgatgtcggc gtggatgcgg gccacgacct cgatgtcgaa gtcgaacctg
     4321 ctcgcccgct cgtcgccgga gtggggcccc gtgcccgtcg tgacgacgcc cgcgaccagc
     4381 agcaccagca tggccccggt cagcacccgc cccagcgtga cgatccacgg atgcactgtg
     4441 ggaaccaccg gcccgtcgcc ctcggtcgac cgcgcgaaca gcatgtaggc cgccgcgatc
     4501 atcccgatcg agaccaggaa gtggacgctg acggtgaccg ggttgagcgc ggtccgcacc
     4561 accagcccgc cccacagtgc ctggaagatc accccgagcg gctggaccag cgcgagcggg
     4621 atgagcgagc gccgcttcag cttccaggcg gccaggaaga ccgcgatggc gacggccagc
     4681 accatgaggc tgaacatccg gttgccgaac tcgatcgcgg tgttgatcgg cgagtggtcc
     4741 ggatgggcga ccgggacgaa actgtcggcg gtgcaccggg gccaggtggg gcagccgagc
     4801 ccggaccccg tgagccgcac ggcggcaccc gtcacggtga tgcccgcgtt gacgacgacc
     4861 gccgccagcg cccaggcccg catgttccgg gcggagggag accagacgga ggagaggaga
     4921 cgcttcacgg cgtcaatgct aggtgcggtg ggtaccggtc cctcttgcgg cctgatatgt
     4981 catgtgacat ggtacaaacc gtggacgtcc tcgtggtggg agccgggatc gtgggcgctt
     5041 cgtgcgcata cttcctgacc cgcgccggtc tgcgggtaac ggtagtcgat cgcgggggag
     5101 tggcgtccgg cacgacgggc gccggcgagg gcaacatcct ggtgtccgac aaggaaccgg
     5161 gccctgaact ggaccttgcg ctgaggtcga cacaactgtg gcgagaactc gccgaggagt
     5221 gcggcggctt cgagtgggac cccaagggcg gcttggtcgt ggccgaaacc caggaggtct
     5281 tggacgccct ggtgatcttc gccgagcgcc agcagcagca cggccggatc gccggatccg
     5341 aagtgggcac cccggaaggc gccgcacctg cgccggccga cgtcgtcgcc acctgcatcg
     5401 cggcggggga cctccgcgaa ctcgaaccgc acatcgccga cggtctcgcc ggtggcgtcc
     5461 actacccgca ggacgcgcag gtgcagccca tgctggccgc cgcgcgcctg ctcaaggcct
     5521 ccggcgcccg gctggtccgg accgccgtag agcgttttct cgtctccggc gaccgggtga
     5581 tcggggtccg taccggtgag ggcgacgtct acgccgacgc ggtcgtcaac gccgccggga
     5641 cctgggccgg aagcttcgcc caggcaccgc tcccgatccg gccgcgtaag gggttcatcc
     5701 tggtcacgca gcctttcgtc gtcccgccga tcaggcacaa ggtctacacg gcggcttaca
     5761 tcaccaacgt cgccagcgac gccgaagggc tggagacgtc gggcgtcatc gagtcgaccc
     5821 aggcagggac cgtgctcatc ggggcctcgc ggcaaagggt cggttacgac acctcgatcg
     5881 actacagggt cctggcgaaa ctggcccggc aggcgatcgg gctgtttccc gtcctggccg
     5941 accggcaggt catgcgggcc tacgcggggc tgcggccgta ctcgccggat catctgccgg
     6001 tcatcgggcc cgactggcgg ctggagaacc tcttccacgc ctgcgggcac gagggcgccg
     6061 gcatcggact ggctccggcc accgccgaga tgatcaccgc gcagatcacc ggcgagccga
     6121 ccccgcgaga tccggcgccc ttcgccccgg ggaggttcgc atgatcgagt tcggtttcca
     6181 gggacgcacg atcacggccg aggagggaca gtcgatcggc gcggcgctgc acgccgccgg
     6241 gatccggtcg tggcggacca cccggttcgg gaaccggccg cgcgggctgt tctgcgccat
     6301 cggcgcctgc ttcgactgcc tgctcaccgt caacggcact cctgaccagc gggcctgcca
     6361 gaccgtcgct cgctccggcg acgaggtgag cccgtgagaa tgcaagccct caggatcgac
     6421 ggctcgaccg aggcccggcc cgtgaagaca tgtcaccaca ccggcgccgg ccaggctgat
     6481 gaggtaggcc catgatctac gatctcgccg tcatcggggc cgggcccgcc ggcctcaccg
     6541 ccgccagggt cgccgccagg cgtgggctca gcgtcgcgct cgtcgacgcc tacgccagaa
     6601 ccggcggtca gtacctccgg ggtgccacgc aaccccgcct gaccggcatc gacgtgaagc
     6661 tgggccagcg ggtgtgggcg gtggagaagt ccggagaggg cttccagatc cacgcgtcca
     6721 ccacgaccac cgcctcgacc ctgctggtcg cgaccggcac ccacgacaag gtcatcccgt
     6781 tcccggggtg ggatctgccg ggcgttctca ccgccggtgg tgcccaggcc ctggtgaagg
     6841 gcgagcgggt caagcccggc gaggagatcg tcgtggcggg caccgggccc ttcctgctgc
     6901 cggtcgcggt cacgctgctc gacgcggggg tgcgcgtcaa ggaggtactg gaggccaacc
     6961 gcccctcgcc ccacatggcc gccgcactgg cccgggtccc gtcgaaggcc agagaggccg
     7021 tcgccttcgc gcgaaggctg gcggacgcgc gggtaccgta cagaacagga cacgccgtgg
     7081 tggccgccca cggcaaggac tccgtcgagg gcgtcaccgt cgccaagatc accaaggact
     7141 ggaagcccta cgcctggcgc accgtcgact gcgacaccct cgccgtcggc tacggcttca
     7201 ccccgcaggt cgacctcctg gtccagctcg gctgccggac cgagggcgat cacgtcgtca
     7261 ccgacgaccg gcagcggacc tccgtcgacg gggtgctggc cgccggtgag gtcgcggggg
     7321 tcgcgggtgc gcaggccgcc gtggtggagg gcgctctggc cgtccacgcc ctgatcggcg
     7381 agccgccgcc ccggctgctc gccgagaagc gccgcctgcg ccgtttcgcg cgggccctgg
     7441 aactctccta tccggtacgc gacggctggc agcactggct cagaccggac accctcgtct
     7501 gccgctgcga agaggtcacg gtcaccgcga tcgaagaggc ccgtgacctc ggcgccgacg
     7561 atcccagaat ggtcaagcta ttggcccggc ccggaatggg ctggtgccag ggccgcatgt
     7621 gcggccacgc cgtcgcccgg ctgaccgggc acgagccagc cccccagaag agaccgttcg
     7681 cccagccgat caccctcgca gacctgacac tgcacgatcg agaggacagc acatgatccg
     7741 accctggcac ggcatcatgg tggccaccgc gctccccttc accggtgacc tcaccgttga
     7801 ccacgacgcc tatgccgcgc actgccgccg gctcgtcgac gagggttgtg acggcgtggt
     7861 gcccaacggc tcgctgggcg aataccagac cctgacgccc gacgagcggg cccgggtggt
     7921 ccggaccgcc gtcgaggccg tcggcggcga ccgggtgatg cctggcgtgg ccgcctacgg
     7981 ctcccgtgag gcgctgacct gggccgagca ggcccgcgac gccgggtgcg ccagcgtgat
     8041 gctgctgccg cccaacgcct accgggccga tgagaacgcc gtgctggagc actaccggac
     8101 cgtcgccaag gcgggcctgc cgatcgtggc ctacaacaac ccgatcgaca cgaaggtcga
     8161 cctgaccccg gcgctgctgg ccaagctgtt cggcgagggc ctgatcgtgg cggtcaagga
     8221 gttcagcggc gacgtgcgcc gcgcctacga gatccgcgag gtggcccccg acctcgacct
     8281 cctcgtcggg tcggacgacg tggtgctgga gctgctcacc gccggggcga agggctggat
     8341 cgccgggttc cccaactcgg tgacccgcca gtgcgtcgac ctctaccggg ccgcggcggc
     8401 cggcgacctg aacaccgccg tgccgctcta ccgggatctg caccccctgc tcagatggga
     8461 cgcacgggta gaattcgtgc aggcgatcaa gctgtcgatg gacgtggccg gcctcaatgg
     8521 cggcgcctgc cggccgcccc gcctgccgct ccctcccgcc acggcggccg agatccgcgc
     8581 cgcgacagag aaggtcatgt gacgtgcgca cccgaaggat cttccacgcc gtcgactccc
     8641 acaccgaggg catgcccacc cgcgtgatca ccggcggcgt cggggtcatc cccggcgcga
     8701 ccatggccga acgccgcgag cacttcatgg cccacctcga tcacctgcgc actctgctca
     8761 tgtacgagcc ccgaggacac gccgccatga gcggcgccat cctccagccg ccgaccaggc
     8821 ccgacgccga ctggggcgtg ctctacatcg aggtctccgg ctgcctgccg atgtgcggcc
     8881 acggcacgat cggcgtggcg accgtactgg tcgagaccgg catggtcgag gtcgtcgagc
     8941 cggtcaccac gatccggctc gacacgcccg ccggtctcgt caccgcgtcg gtcgcggtcc
     9001 gcgacggcgt ggccgagtcg gtcacgatcg agaacgtgcc gtccttcgcg gtcgacctcc
     9061 aaaagacgat caaggtcgac gggttcggtg aactcaccta cgacctggcc tacggcggca
     9121 acttctacgc catcctgccg atcgagtcgg tggggctgcc gttcgaccgg gcgtacaagc
     9181 agcagatcct ggacgcgggt ctcgcgatca tggatgtgat caatgagaac gagcccgtcc
     9241 acccggagga ttccggcatc cgcggctgcc accacgtgca gtttctcgcc cccgggtcca
     9301 ccgcgcggca ctcgcggcac gccatggcga tctaccccgg ctggttcgac cgctcgccgt
     9361 gcgggaccgg caccagcgcc cggatggccc agctccacgc cctcggcgaa ctgcccattg
     9421 acgccgaatt cgtgaacgag tcgttcatcg ggacgcggtt caccggacgc ctggtcgcga
     9481 ccgccacggt cgaagatcgg caggccgtgg tgccgcacat caccggtcgc gcctggctca
     9541 ccggcaccgc ccagtacttc cttgatcccg gggacccgtt tccgcagggt ttcctcctgt
     9601 gacctcgcgg tcgcgtaccg gtccgaaccg gtacgatcac caggtccgac gtcacggatg
     9661 ggagtggaat ggccacagca gaggggctcg gcctgccggt cgtaggtgag cggcagagcc
     9721 tgcgcgaaca ggtcgcgcaa gccctcagag acgcgctggt cgcgggtgag atgaggccgg
     9781 gggtcgtcta ctccgcgccg gtgctcgccg cccagttcgg cgtctcggcg acccccgtcc
     9841 gggaggcgat gctcgacctt gccaaagaag gcctggtcga ggcggtgcgc aacaagggct
     9901 tccgggtcac cgagatgtcc gacaccgatc tcgaccacat caccgagatc cgccgcctca
     9961 tcgaggtgcc cacggtcgcc aagctggccg ggtcggggct cagcgccgac ttcgaacggc
    10021 tgcgcccgct ggcccgcgag atcgtcaccg ccgccgaacg cggcgacctg ctcacctatg
    10081 tcaacgccga cctgcgtttc cacgtcgagt tgctgggtct gaacggcaac gcccacctgg
    10141 tcgaggtggt ccgcgaacta cgcagcaggg cccgcctcta cggcctgaaa agcctggccg
    10201 acgccgggaa actggtcgac tcggcccgcg aacacgtcga actgctcgat gccctgatcg
    10261 ccggcgacag ccgtgcggca gaacacgtga tgaaacacca cattcagcac gtgcgcggca
    10321 tatgggccaa caagccggag tagttcaccc cgaacgtgcg gccccgatcg aggcgaccac
    10381 cacacacccg atcgcggccc actcccgcag atgcaggacc tcgcccagca cgaccagccc
    10441 gaccatcgcg gcgacggcgg gttcgaggct catcagaatg ccgaacaccc gtttgggcat
    10501 ccgccgcagc gcctcgagct ccagcgagta ggggatcacc gacgacagca gcccgacgcc
    10561 gagcccgatc agcaggatcg tggggtcgag caggtccaga cctccggagg cgatccccgg
    10621 cggcgccacc accacggtcg cgatgatcat ggcgaacacg agccccgacg tgccggggaa
    10681 ccgctgcccc accgacgccg acagcaggat gtacgccccc cagcagaccc ccgccgccgc
    10741 ggcgaacccg atcccgaccc agtcggcgtt ggcggcgttc ccccacggcg cgagcagcac
    10801 gatcccggtc ccggccagca ccacccacac cacgtcgagc ttgcggcgcg aggcggccac
    10861 ggccaccccc agtggtccca tgaactcgat ggccaccgcg atcccgatcg ggagcctggc
    10921 cagcgcctcg tagaaggtca ggttcatcac cgcgaggatg accccgaacg ccagcccgat
    10981 cctgacgctc tgccacgaga gccccctgag cctgggccgg acgaacgcga cgagcaccag
    11041 cgtcccggtg gcgatgcgga ggaagaccac ggccgtgggt gagagctggg cgaagaggtc
    11101 tttggccagc cccgccccga tctgcaccga gacgatgccc agcatcacca gcgcgggcgg
    11161 cggaacggct ccggcggccc tggtccacgg cttggacact tccgtgtcag cggtcaccct
    11221 gtccactcct caccccgaaa ccagcggtca gcagtgtaat gggcaaacac cggcattgct
    11281 gctgatatcc gatcggtgag acgcccctcg gagctactcc cagcggaacc agcgggccgc
    11341 cgccgcgagc gccagtacgg cccacgacag gagcaccagc gcggccccga tcgggaaccc
    11401 gccgaccccg gtcagcgtcg agcgcagccc gtcggtcagc gccgtgatcg gcaggatctc
    11461 cagcagcggc cggatgcctt cggggaacgc cgtgatcggc agcaccaccc cgcccagcgc
    11521 gagcagcagc aggtagacca ggttggcccc ggccagcgtc gcctcggcgc gcagcgtgcc
    11581 ggccatcagc agcccgaggc cgctgaaggc cgccgtgccg aggatcacca ggagcagcac
    11641 ggtcagcgga ttgccgtgcg gcgaccagcc cagcgccagc gcgaccagca ccagcacggc
    11701 gatctgcagc agttccacca cgatcacggc ggtggtcttg gccagcatca accccgtccg
    11761 agacagggga gtggccccca gccgcttcag cacgccgtag cgccgctcga agccggtcgc
    11821 gatggcctgc ccggtgaacg ccgtcgacaa gaccgccagg gcgatcacgc cgggcgccac
    11881 gaagtcgacc ttggggcccg cgacgtcgag gaaaggcgcc ttggagaacc ccaccagcag
    11941 caggatcggg atgatcaggg tcagcaggag ctgctcgccg ttgcgcaggg tggcgcggac
    12001 ctccgcgccc gcttgggcga gcaccatccg cgcgagcggc gccgcgccgg gcgccgggga
    12061 caggtcgagg gtggtcatcg cagttcccgt ccggtcagtt ccaggaagac gtcttccagg
    12121 gtgcgccgct cgatgctgag gtcttcggtc gtcacgccct cggtcgcgca ccacgcggtc
    12181 acggtggcca gcagcgacgg gtcgacgtgg ccctcgatga tgtagtggcc cgccggtgac
    12241 tccttggcgg ccgagcccgg gggcagcgcg ctgagcagtt cgtcgaggtt cagcccgggg
    12301 cgggcgcgga agcggagctg gcgttcggcg ccggtcagct cggcgggcga gccctcggcg
    12361 accacgcggc cccggtcgat gatgacgacg tggtcggaca gcttctctgc ctcgtccatg
    12421 tggtgggtgg tcagcaccac cgacaccccg gccgcgcgca gttcgcggac catgtcccag
    12481 caggcgtgcc gggcctgcgg gtcgagcccg gccgtcggct cgtcgaggaa gaccagttcg
    12541 ggacggccga cgacggccgc ggcgagcccg agccgctgct gctggccgcc ggacaggcgc
    12601 cggtaggggg tgcgggcgtg ttcggcgagc cccagccgtt cgagcagggc cctggggtcg
    12661 agcgggttcc ggtggaaccg ggagaccagc cgcagccact ctccggcgcg ggccgcctgc
    12721 ggtgtgccgc ccgact
//
