LOCUS       NZ_BBXD01000012        13724 bp    DNA     linear   CON 08-AUG-2024
DEFINITION  Herbidospora mongoliensis strain NBRC 105882, whole genome shotgun
            sequence.
ACCESSION   NZ_BBXD01000012
VERSION     NZ_BBXD01000012.1
KEYWORDS    WGS; HIGH_QUALITY_DRAFT; RefSeq.
SOURCE      Herbidospora mongoliensis
  ORGANISM  Herbidospora mongoliensis
            Bacteria; Actinomycetota; Actinomycetes; Streptosporangiales;
            Streptosporangiaceae; Herbidospora.
REFERENCE   1
  AUTHORS   Komaki,H., Ichikawa,N., Oguchi,A., Hamada,M., Tamura,T. and
            Fujita,N.
  TITLE     Genome-based analysis of non-ribosomal peptide synthetase and type-I
            polyketide synthase gene clusters in all type strains of the genus
            Herbidospora
  JOURNAL   BMC Res Notes 8, 548 (2015)
   PUBMED   26452464
  REMARK    DOI:10.1186/s13104-015-1526-9 Publication Status: Online-Only
REFERENCE   2  (bases 1 to 511105)
  AUTHORS   Hosoyama,A., Oguchi,A., Ichikawa,N., Kimura,A., Ohshita,R. and
            Fujita,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAY-2015) Contact:Director-General Department Of
            Biotechnology National Institute of Technology and Evaluation,
            Biological Resource Center (NBRC); 2-49-10 Nishihara, Shibuya-ku,
            Tokyo 151-0066, Japan URL :http://www.nite.go.jp/nbrc/index.html
COMMENT     ##Genome-Assembly-Data-START##
            Finishing Goal                    :: Noncontiguous Finished
            Current Finishing Status          :: High-Quality Draft
            Assembly Method                   :: newbler v. 2.6
            Genome Coverage                   :: 62x
            Sequencing Technology             :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_001570545.1-RS_2024_08_08
            Annotation Date                   :: 08/08/2024 02:40:32
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,280
            CDSs (total)                      :: 8,202
            Genes (coding)                    :: 8,113
            CDSs (with protein)               :: 8,113
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 6, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 4, 5 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 21 of 89
            Pseudo Genes (incomplete)         :: 71 of 89
            Pseudo Genes (internal stop)      :: 4 of 89
            Pseudo Genes (multiple problems)  :: 7 of 89
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 18:23:16
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 497381
            Orig. end                         :: 511105
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            BBXD01000012.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..13724
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="terpene"
                     /region_number="2"
                     /rules="(PT_phytoene_like or phytoene_synt or Lycopene_cycl
                     or Lycopene_cycl_fung or T1TS or T1TS_KS or T2TS or TS_UbiA
                     or TS_Pyr4)"
                     /tool="antismash"
     cand_cluster    1..13724
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(PT_phytoene_like or phytoene_synt or
                     Lycopene_cycl or Lycopene_cycl_fung or T1TS or T1TS_KS or
                     T2TS or TS_UbiA or TS_Pyr4)"
                     /kind="single"
                     /product="terpene"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..13724
                     /aStool="rule-based-clusters"
                     /category="terpene"
                     /contig_edge="True"
                     /core_location="[10000:10906](-)"
                     /cutoff="20000"
                     /detection_rule="(PT_phytoene_like or phytoene_synt or
                     Lycopene_cycl or Lycopene_cycl_fung or T1TS or T1TS_KS or
                     T2TS or TS_UbiA or TS_Pyr4)"
                     /neighbourhood="10000"
                     /product="terpene"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(10001..10906)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(PT_phytoene_like or phytoene_synt or
                     Lycopene_cycl or Lycopene_cycl_fung or T1TS or T1TS_KS or
                     T2TS or TS_UbiA or TS_Pyr4)"
                     /neighbourhood="10000"
                     /product="terpene"
                     /protocluster_number="1"
     misc_feature    complement(1401..1417)
                     /note="TFBS match to GnfM, Regulator of nitrogen-fixation
                     genes, confidence: weak, score: 19.16"
                     /tool="antismash"
     misc_feature    1409..1429
                     /note="TFBS match to NrtR, NAD synthesis repressor,
                     confidence: medium, score: 27.36"
                     /tool="antismash"
     gene            complement(1434..2873)
                     /locus_tag="AW272_RS17155"
     CDS             complement(1434..2873)
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1041:
                     transcriptional regulator, SARP family"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012889319.1"
                     /locus_tag="AW272_RS17155"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="BTAD domain-containing putative transcriptional
                     regulator"
                     /protein_id="WP_066365827.1"
                     /transl_table=11
                     /translation="MVILRALGPFQAVSDGRPLDLGGQRQQAVLARLLVAGGRAIPVST
                     LIDELWPGRPPVQALSTIQGYVSRLRRALEPGRAPREEAGVLVSAPPGYALRADLADVD
                     VWHFEHLVKRDDAGDLEATLTRLDTALALWRGPALAEFGDLAWAQAEAQRLGDLRLIAI
                     ERRADAAVRLGRSAALIPDLEAHASAHPLREESWRLLAVALYRVGRQADALGALRRARD
                     VLRDDLGLDLGPTLQRLEQDILGQAAHLDGPAPAAPVVARPATGVLRVVVADDQALVRT
                     GLKVVLDSEPGLEMVAEAENGEQAIQQVQLTRPDVVLMDIQMPRLDGLAAARRILAGND
                     PPKVIMMTTFGTDENLYSALRAGVSGFVLKTSAPEQLIAAVRAAVAGDALIDPAVTTHL
                     IAAFAGRQNPAAPGGLSDTEVDLVRLIARGFTNRQIAVTLAVEESEVAAEVAGLLKRLD
                     LIDRAQLVVLAYESGLVSPGT"
     gene            complement(2898..4886)
                     /locus_tag="AW272_RS42070"
     CDS             complement(2898..4886)
                     /GO_function="GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA]"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1048: sensor
                     histidine kinase"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /locus_tag="AW272_RS42070"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="histidine kinase"
                     /protein_id="WP_157518590.1"
                     /transl_table=11
                     /translation="MEDRGSGQALVIAAVVGAVSVVVVGAAVWIHFQLPPNVRESLSTT
                     PHYTAGATFPAVGAYIAARRPRLMIGRLMIVGGLLAGLTVLFLALTTRAAVDGDLAAAG
                     VFRIVTVASWALADGFLAIWVPLFAIGGRRPSRGWSVFAVVAGVIFVTRALLWVGEPVP
                     EGPLPPGSVVVPNPLEFVPQGLYPPLNQVLFYAVLACIVLALVSMVVRIRGADPEVRRA
                     VAWPALTFAVYVVLLVIDNPGHWPFLTVWTALIPVSIMFSVLRHGLFGIDNVLSRTVVV
                     AGTIAGVSAVYFGTGAVFSLLMSEYDIVAGLAAALFAGVFYQPLRRLLQRGMDRLLYGP
                     VGDPRMLTARLVEEVRNADPSEALAAVVDVVRDGLAVDGVAVEVVNGLHVSSGQVGDGP
                     KEHPLVWHGEPVGRLLLGRPGSRRFGAAHDERVIAALLPYVADVAHAVRMASDLQRSRE
                     RILAAREEERRRLRRDLHDGLGQTLGAMAMTLNMARLTIKGSPGTADHLLRDLRTGMDA
                     VAGDIRELVYGLRPPALDDLGLIEAIRTLATETLDPGTHISVDGPAADLPAAVEVAAYR
                     IAQEALTNVRKHAHARRVSILLTLDDGLLTVRIADDGIGLPEVRRAGVGTSSMRERAAE
                     LGGSCAITAPDTGGTVVTARLPLTGTM"
     misc_feature    4899..4919
                     /note="TFBS match to NrtR, NAD synthesis repressor,
                     confidence: weak, score: 19.27"
                     /tool="antismash"
     gene            complement(4914..6128)
                     /locus_tag="AW272_RS17165"
     CDS             complement(4914..6128)
                     /GO_function="GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1202: major
                     facilitator transporter"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020541533.1"
                     /locus_tag="AW272_RS17165"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="MFS transporter"
                     /protein_id="WP_066365829.1"
                     /transl_table=11
                     /translation="MTAPPVERSPVRAFVHTRVGGLPREFWVLFSGTAINKLGTMAQPL
                     MGIYLTQSRGVSVATTGVILTLWGCGSLLSQPIAGFLADRVGRRATLAGGMTATAATMI
                     FLGYAWDLTAIAAGMFVLGLVVDTYRPASQALLADVVSPEDRPRAFGLLFWAINLGFSI
                     AMVAGGRLASSGFLLLFWVDAASCLIFAYLVWRLIPETRPPAAERLPGGYRDVLRDRTM
                     VAFVLMNLAYTCTYLQAFQTMAIAMTQRGLSPEAYGTAIAVNGLLIILVQPITTAWLVR
                     FSPSLVLAAGFAVVGVGFGLQAFATTTLEYALAIVVWTCGEILTAGMPLAITSALAPPH
                     LRGRYNGLYGLSWSVGAAVTPMLGTRLLEIGHEALWLTTGALGLVAAIGQIILAPAIRH
                     RTKTQ"
     gene            6235..7200
                     /locus_tag="AW272_RS17170"
     CDS             6235..7200
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020541534.1"
                     /locus_tag="AW272_RS17170"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="DUF5937 family protein"
                     /protein_id="WP_066365832.1"
                     /transl_table=11
                     /translation="MAVEWSFTPGDVARLRFAFSPMWEVVASLRALQGAALHTPQVPWL
                     RAVRDRLTALGLGDLTGLVPAAGYVPDFLTPTPDTHQPDFAAELALVRATEPRVARAEV
                     ARSAPRRAGMAARFADDPAGALAWTGDALESYWNLIFAEFWPKVRGVLEADVLYRSRRL
                     TEGGVRDLFAALHPSVTWHGDRLIADKGCIHWGELGGAGLVLLPSVFQPLDRVSVMHEP
                     YQPMLIYPARGMGNLWARSPEEVPGALSALIGRTRARILTALESPASTSGLARVMELTP
                     GAVSQHLTVLSDGGLVSRQRLGREVLYRRTAIGDALVGEP"
     gene            complement(7197..9131)
                     /locus_tag="AW272_RS17175"
     CDS             complement(7197..9131)
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA]"
                     /GO_function="GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /GO_process="GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1048: sensor
                     histidine kinase"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /locus_tag="AW272_RS17175"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="histidine kinase"
                     /protein_id="WP_083958387.1"
                     /transl_table=11
                     /translation="MTVASHEVETRPKWMRVPDRAARGLAVGIAGAAILLVVADLVISS
                     RLPELEWPLPVTPEDVMGVAFPVFGALLVQRRPKLVIGWLLTLGGLGAAANIVSMDLAL
                     LLDRDTLTTPLAVLSHGVWNLTTYALGVLLPMLYPTGALLSRKWCWAGGFAAAVLAADW
                     IVDIIGPKSLAIPAFAPYAPWSEGILRHLVTVSLVIAFSSLVIRFRRAEGDERRQILWP
                     LLAIACLSVPWIIGNSVWWVTAITIPLIPAAITLAVLRYRLFGIDTLITRALVGAGMVT
                     VFASVYVLAGTASSLFLAEVDRYFGLVAALGAGVFFHPMKRALHRLADRALYGTGGDPV
                     TLADGLRRRLQRTDPVHGLLATLETIREGLAVTGAALDLDGTRTDWGELPVVARRVDLV
                     WHGEPVGTLLIGPPDRRRFPAAHDERVIAALTPYIADAAHTMRLTTALRKSRERILTAR
                     EEERRRLRRDLHDGLGQTLTAMAMRITTARLNLKGSPEITDTLLADLRCGMDAVSGDIR
                     ELVYGLRPPALDDLGLAGAVKDLGPDALTVDGDLTGLPAAVEVAAYRIAQEALTNIHRH
                     AAATHAAITITRGVDLVLEIRDNGQGMPLDAVPGVGLSSMRERAAELGGVCEIDTSGEG
                     TTITARLPL"
     gene            complement(9193..9957)
                     /locus_tag="AW272_RS17180"
     CDS             complement(9193..9957)
                     /GO_function="GO:0030248 - cellulose binding [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF041144.1"
                     /locus_tag="AW272_RS17180"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="expansin EXLX1 family cellulose-binding protein"
                     /protein_id="WP_157518591.1"
                     /transl_table=11
                     /translation="MRHARRRRRRGVGFAVGALVAAAVFVRLTDPVCASPGPQRGRAYF
                     YEAKAPGNCSLPGHPMLFASVSTAEYAGSAACGGYLDVTGPTGTVRVLVVDRCPACGPG
                     DLDLSAEAFVSIAHKSDGSAAITYRQVRDPEPGRRLAFRVKPGSTAHWGAVQVLDHGNR
                     LKHVRVRSGAHWRDLRRGPDNYWLFPPGPGPGPYTMRVSDVFGGQATVTGVRLRPGATQ
                     RFGERLYGTPPPKVRTITTPSDKPSPEMPEIC"
     gene            complement(10001..10906)
                     /locus_tag="AW272_RS17185"
     CDS             complement(10001..10906)
                     /GO_function="GO:0010333 - terpene synthase activity
                     [Evidence IEA]"
                     /GO_function="GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /GO_process="GO:0046246 - terpene biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     terpene: T1TS"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1052: Terpene synthase/cyclase metal-binding domain
                     protein"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF040195.3"
                     /locus_tag="AW272_RS17185"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="terpene synthase family protein"
                     /protein_id="WP_066365836.1"
                     /sec_met_domain="T1TS (E-value: 3e-38, bitscore: 127.1,
                     seeds: 237, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTPELRIPSAAAVLAPLVYALPPTAGEPVECDTRALGAWAGLYGL
                     SIAARPERLAARVLPGNELLARWVTWQLAYDDWCDEGSGSRDTTVLDLMQGRLRDAAHA
                     GHRTEEPLVAAFADLWRVTAPDMSPAWEQRFTARMEAQAQACRMQAVNRSIGRVPSAGE
                     YPALRRDVFAGAGAEIIEGCLRIEVPGRLHATPPWRTLLDAVVDVTAWCNDVASLGKER
                     GDPHNYLRVAASRIGVPAPAEWVADRIVERAEDLTDAAKALPEAYAELGVPDEVEKVAS
                     SLFGVPRAYLEWLLESPRYQ"
     gene            11049..12410
                     /locus_tag="AW272_RS17190"
     CDS             11049..12410
                     /EC_number="6.3.1.-"
                     /GO_function="GO:0004356 - glutamine synthetase activity
                     [Evidence IEA]"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_process="GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013151975.1"
                     /locus_tag="AW272_RS17190"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="glutamine synthetase family protein"
                     /protein_id="WP_066365838.1"
                     /transl_table=11
                     /translation="MDRQQEFVLRTLEERDIRFIRLWFTDVLGFLKSVAIAPAELEGAF
                     AEGIGFDGSAIEGFSRVYESDMLAKPDPSTFQILPWRSETPGAARMFCDILMPDGSPSH
                     ADPRWVLKRTLARAADMGFTFYTHPEVEFFLLRNRPERGVKPEPIDDGGYFDHTPHSAG
                     HDFRRNAIMMLEAMGISVEFSHHEGAPGQQEIDLRYADALSTADNIMTFRLVMKEVALE
                     QGVWASFMPKPFTEYPGSGMHTHMSLFEGDRNAFYEPGSDYQLSKVGRSFIGGLLKHAA
                     EITAVCNQWVNSYKRLWGDTEAIAGAGGEAPSYICWGHNNRSALVRVPMYKPHKGGSTR
                     IEFRSLDSACNPYLAYAMILAAGLKGIEEGYDIPAGAEDDVWALTSGERRALGIHPLPQ
                     SLDEAIVAMEHSELAAETLGEHVFDYFLRNKRSEWREYRRQVTEFELGRYLPVL"
     gene            complement(13152..>13724)
                     /locus_tag="AW272_RS17195"
     CDS             complement(13152..>13724)
                     /codon_start=1
                     /gene_functions="other (smcogs) SMCOG1238: transposase"
                     /gene_kind="other"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012894646.1"
                     /locus_tag="AW272_RS17195"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="transposase"
                     /protein_id="WP_218010916.1"
                     /transl_table=11
                     /translation="AWLCFEDEAAQTLRSPKGRTWGRRGITPVVAVTAKGGGRISIAAL
                     VAVKPADPAAGARLIYRTRPYRGRKGEPKGFAAADYAALFDAAHQQLGAPLVVVWDNLN
                     THHSARMRELIAARPWLTVFYLPAYAPEFNPVEAVWAHMKKSLANLATRNQDQLLALVK
                     SRLKRMQYRRRILSGFIAKTGLDPHLS"
ORIGIN
        1 ggcacgtcct tgatcggcgc gtagccgccc gccgagtcgc cgtagagggt cgagtagccc
       61 gccgccagct cgctcttgtt gccggtggtg agcaccaggt ggccgtgctg gttcgacagc
      121 gacatcagga gcatgccgcg tacgcgggcc tggaggttct cggccgccag cccggtcagc
      181 tcgacctcct tctcgaacgc ggccaccacg tcggcgatcg gcacgatccg ggagtggatg
      241 ccctggcggc ggaccaggtc ttcggcgtcg gcgatcgagt ggtcggagct gtagcgcgag
      301 ggcatcagca cgacgtggac ccggtcgggg ccgatggcgt cggcggcgat cgtggcggtc
      361 agggccgagt cgatgccgcc cgacaggccc aggatgaccg agcggaagcc gttcttctcc
      421 acgtagtcgc gcaccccgag cacgagggcg ccgtagacct cggcggcgtc gtcgaggcgt
      481 tcggcgatca ccgagggccg cgggtcgaac gtgaccggct cctccgacag gatcagccgc
      541 tcgaccgtga tcgaggtgcc gtcgccggcg tcgtacacga cctcgcccgg ttcaccggaa
      601 cccagcggca ggtcgaggtc ggcgaagagg agttcctcgg tgaactgggc cgcgcgggcc
      661 aggagcgttc cgtcggcgcc gaccaccagg gagtcgccgt cgaagaccag ttcgtcctgc
      721 ccgccgacct ggttggcgta ggcgagggcg cagcccgcct cgacggcccg ccgtgcgcac
      781 aggtcgcgcc gcacgtcgtc tttctcccgc tcgtacggcg aggcgttcgg caccaccaga
      841 agacccgcgc cggccgctcc ggccaccgac accgggccgc cctcctgcca caggtcctcg
      901 cagatcgcga cggccacgtc gacgccgtgg aggcggaaga ccggcagccg gtcgccgcgc
      961 acgaagtagc ggtattcgtc gaagacgccg tagttgggca ggtggtgctt ggcggtgcgg
     1021 gcgaccaccc ggccctggta gagcacggcg gcggcgtcga ggggggcgcc cttgggctgg
     1081 cccacccggg ccacccggtc ggcccggtcg acgtagccga cgaccacggg aagatcgccg
     1141 tatccggcct cggcgaggcg gcgggccgag acagaaaggg cctcgatcga cgcgtcgacg
     1201 aacgaggcgc gcaggaccag gtcctcgacc ggatagccgg tcaggaacat ctccgtgaag
     1261 agcaccaggt gggcgccgcc cgcgtgggcc ctgccggtcc attcgacgat ccggtcggcg
     1321 tttccggcga ggtcgccgac gacggggttg gtctgggcga gtgcgatgcg gagttgagcc
     1381 acgagaccag cctacggtga cgccccggtt ttcatcaatg tgacgataag cacctaggtt
     1441 ccggggctga cgagtcccga ctcgtaggcc agcacgacca gctgggcgcg gtcgatcagg
     1501 tcgagccgct tcagcagccc ggcgacctcg gcggccacct cggactcctc gacggccagc
     1561 gtgacggcga tctggcggtt ggtgaagccc cgggcgatga gccggacgag atcgacttcc
     1621 gtgtccgaca gaccgccggg cgcggcgggg ttctggcggc ccgcgaaggc ggcgatgagg
     1681 tgggtcgtca cggccgggtc gatcagcgcg tcgcccgcca cggcggcccg gaccgctgcg
     1741 atgagctgct cgggggcgga ggtcttcagg acgaagccgc tgaccccggc gcgcagcgcg
     1801 gagtagaggt tctcgtcggt gccgaaggtg gtcatcatga tcaccttcgg cggatcgttc
     1861 ccggccagga tccggcgcgc cgccgccagg ccgtcgaggc gcggcatctg gatgtccatc
     1921 agcacgacgt cgggccgggt cagctggacc tgctggatcg cctgctcgcc gttctcggcc
     1981 tcggccacca tctccaggcc gggctcgctg tccagaacca ctttcagtcc ggtgcgcacc
     2041 agggcctggt cgtccgccac caccacccgg agcacacccg tcgccggacg cgccacgacc
     2101 ggggccgcgg gggccggccc gtcgaggtgg gcggcctgcc cgaggatgtc ctgctccagc
     2161 cgctggagcg tcggccccag gtcgagcccg aggtcgtcgc ggagcacgtc gcgggctcgc
     2221 cggagcgcgc cgagcgcgtc ggcctgccgc ccgacccggt agagggccac cgcgagcagc
     2281 cgccaggact cctcgcgcag cgggtgggcc gaggcgtgcg cctccagatc ggggatgagc
     2341 gcggcgctgc ggcccagccg gacggccgcg tcggcccgcc gctcgatcgc gatcagccgc
     2401 aggtcgccga gccgctgagc ctcggcctgg gcccaggcca ggtcgccgaa ctcggccagc
     2461 gccgggcccc gccacagcgc cagcgcggtg tcgagccggg tgagcgtggc ctccaggtca
     2521 ccggcgtcgt cgcgcttcac caggtgttcg aagtgccaga cgtcgacgtc ggcgaggtcg
     2581 gcgcgcagcg cgtatccggg cggtgcggag accagcaccc cggcctcctc acggggcgcc
     2641 cggccgggct ccagggcccg gcgcagccgc gacacgtagc cctggatcgt cgacagggcc
     2701 tggaccggtg gccgccccgg ccacagctcg tcgatcaggg tgctgacggg gatcgcccgc
     2761 ccgcccgcca ccagcagtct ggccaatacg gcctgctgcc gctggccccc cagatcgagt
     2821 ggccgcccgt cggaaacagc ctggaacggc cccagggccc gaagtatcac catggtggtg
     2881 gtaagcgtag agcaacgtca catggtgccg gtcaagggaa gccgggccgt gacgaccgtg
     2941 ccgcccgtgt cgggcgccgt gatcgcgcac gacccgccga gctcggcggc ccgttcccgc
     3001 atcgacgagg tgcccacccc ggctctgcgc acctcgggca gcccgatccc gtcatcggcg
     3061 atccggaccg tcagcaggcc gtcgtcgagc gtcagcaaga tcgatacccg tctggcatgg
     3121 gcgtgcttgc gcacgttggt gagcgcctcc tgcgcgatcc ggtacgccgc cacctccacc
     3181 gccgccggga ggtcggccgc ggggccgtcg acggaaatgt gggtgccggg gtcgagggtc
     3241 tcggtggcca gcgtccggat cgcctcgatc aggccgaggt cgtcgagcgc gggcgggcgc
     3301 aggccgtaga cgagttcgcg gatgtccccg gccaccgcgt ccatgcccgt ccgcaggtcg
     3361 cgcagcaggt ggtcggcggt cccgggggag cccttgatgg tgagcctggc catgttgagc
     3421 gtcatcgcca tcgcgcccag cgtctggccg agcccgtcgt gcaggtcgcg gcgcagccgc
     3481 cggcgctcct cctcgcgggc ggccaggatg cgttcccggg aacgctgcag gtcactggcc
     3541 atccggacgg cgtgcgcgac gtcggcgaca tacgggagca aggcggcgat cacgcgttcg
     3601 tcgtgggcgg cgccgaacct cctgctgccg ggccggccca ggagcaggcg gccgaccggt
     3661 tcgccgtgcc agaccagcgg gtgctccttg gggccgtcgc cgacctggcc cgaggagacg
     3721 tgcaggccgt tgacgacctc gaccgcgacg ccgtcgaccg ccaggccgtc gcggaccacg
     3781 tcgaccacgg cagccagcgc ctccgacggg tcggcgttgc gtacctcctc caccagccgg
     3841 gcggtcagca tccggggatc gccgacggga ccgtagagga gccggtccat gccccgttgc
     3901 agcaggcggc gcagcggctg atagaagacg ccggcgaaga gcgcggcggc cagaccggcg
     3961 acgatgtcgt actccgacat gagcaggctg aagacggccc cggtgccgaa gtagacggcg
     4021 ctgaccccgg cgatcgtccc ggccacgacg acggtccggc tgagcacgtt gtcgatgccg
     4081 aacaggccgt gccgcagcac cgagaacatg atcgacaccg ggatgagggc ggtccagacc
     4141 gtgaggaacg gccagtggcc cggattgtcg atcaccagga ggacgacgta gaccgcgaag
     4201 gtcagggccg gccaggcgac ggccctgcgg acctccgggt cggccccgcg tatccgcacc
     4261 accatcgaga ccagcgccag cacgatgcac gccagcacgg cgtagaagag cacctggttc
     4321 agcggcgggt agagcccctg cgggacgaac tccagcgggt tcgggacgac gacggacccc
     4381 ggcgggagcg gcccctcggg gaccggttcg ccgacccaca gcagcgcccg ggtcacgaag
     4441 atcaccccgg cgacgaccgc gaacacgctc cagccccggg agggccgccg gccgccgatg
     4501 gcgaacagcg gcacccagat cgccaggaag ccgtcggcca gcgcccacga cgcgacggtc
     4561 acgatccgga agacgcccgc cgccgccagg tcgccgtcga ccgcggcccg tgtcgtcagc
     4621 gccaggaaca gcacggtcaa tcccgcgagc agcccgccga cgatcatcaa ccggccgatc
     4681 atcagccggg gccgccgggc cgcgatgtag gcgcccacgg ccgggaacgt cgcccccgcc
     4741 gtgtagtggg gggtcgtgct gagggactcc cggacgttcg ggggaagctg gaagtggatc
     4801 cacacggccg cgccgacgac gacgacggag accgcgccga cgacggccgc gatcaccagt
     4861 gcctggcccg aacctctgtc ctccatttcg ggcaggatat tgaggaatta tgactattgg
     4921 gtcttggtcc tgtgccggat cgccggggcg agaatgatct gtccgatcgc ggccaccagc
     4981 cccaatgcgc ctgtggtcaa ccagagcgcc tcatgtccga tttccagcag tcgcgtgccg
     5041 agcatcgggg tgaccgccgc gccgaccgac cacgagagac cgtagagccc gttgtaccgt
     5101 ccccgcagat gcggcggcgc gagcgcggac gtgatcgcca ggggcatccc ggcggtgagg
     5161 atctccccgc aggtccagac gacgatggcc agggcgtatt cgagggtcgt ggtggcgaac
     5221 gcctgcaacc cgaaccccac gcccaccacc gcgaacccgg ccgccagcac caggctcggg
     5281 ctgaacctga ccagccaggc cgtggtgatc ggctgcacca ggatgatcag cagcccgttg
     5341 accgcgatgg ccgtgccgta agcctccggc gacagccccc gctgggtcat cgcgatcgcc
     5401 atcgtctgga acgcctgcag gtaggtgcac gtgtacgcga ggttcatcag cacgaacgcg
     5461 accatggtcc ggtcgcgcag cacgtcgcgg tagccgcccg gcagccgttc cgccgcgggc
     5521 ggccgggtct ccgggatcag ccgccagacc agataagcga agatcaggca actggccgcg
     5581 tccacccaga acagcagcag gaacccgctc gacgccagcc tgccgccggc caccatggcg
     5641 atcgagaacc ccaggttgat cgcccagaag agcagcccga acgcccgcgg ccggtcctcc
     5701 ggcgacacca cgtcggccag cagagcctga gaagccggtc tgtacgtgtc cacgaccagc
     5761 cccagcacga acatgccggc cgcgatggcg gtcaggtccc aggcgtaccc gaggaagatc
     5821 atggtcgccg cggtcgccgt catcccgccc gcgagggtcg ccctgcgccc gacccggtcg
     5881 gccaggaacc ccgcgatcgg ctgcgacagc agcgacccgc atccccagag cgtgaggatg
     5941 accccggtcg tcgcgaccga gacgccccgc gactgggtca ggtagatgcc catgagcggc
     6001 tgggccatcg tgccgagctt gttgatggcg gtaccggaga agagcaccca gaactctcgg
     6061 ggcagcccgc cgacacgggt gtggacgaag gcacggacgg gggagcgttc gacggggggc
     6121 gcggtcatgg ggacaagcct cgtgagctgg tacgggatga agcgaacctt ttagcccgtg
     6181 ctaaaacatg gcctgaagtc ggtaaatatc gtcgcgggga gacgaggttc acacgtggct
     6241 gtcgagtggt cgttcacgcc gggggacgtg gcgcggttgc ggttcgcctt ctcgcccatg
     6301 tgggaggtcg tcgccagcct ccgcgccctc cagggggccg cgctgcacac cccgcaggtg
     6361 ccgtggctgc gggcggtgcg cgaccggctc accgcgctgg ggctgggcga cctgaccggg
     6421 ctggtgccgg ccgccggata cgtgcccgac ttcctcaccc ccacgcccga cacccaccag
     6481 cccgacttcg ccgccgaact cgcgctggtc cgggccacgg aaccccgtgt cgcgcgggcc
     6541 gaggtggccc gatccgcgcc ccggcgcgcg gggatggccg cgcgcttcgc ggacgacccg
     6601 gcgggggccc tcgcctggac gggggacgcg ctggagtcgt actggaacct gatcttcgcg
     6661 gagttctggc cgaaggtgcg cggggtgctg gaggccgacg tgctctaccg gtcgcggcgg
     6721 ctgaccgagg gcggcgtgcg cgacctgttc gccgcgctgc acccgtcggt gacctggcac
     6781 ggcgaccggc tgatcgccga caaggggtgc atccactggg gtgaactggg cggcgcgggg
     6841 ctggtgctgc tgcccagcgt gttccagccc ctcgaccgcg tctcggtcat gcacgagccc
     6901 taccagccga tgctgatcta tccggcccgg gggatgggga acctgtgggc gcggagcccc
     6961 gaggaggtcc ccggcgcgct gtcggccctg atcgggcgga cccgggcgcg cattctgacc
     7021 gcgctggaga gcccggcgtc gacgtcgggg ctggcccgcg tcatggagct gacgccgggc
     7081 gcggtctcgc agcacctgac cgtgctgtcg gacggcgggc tggtgtcccg gcagcgactc
     7141 ggccgcgagg tcctctaccg gcgtaccgcc atcggcgacg cgttggtggg cgagccttag
     7201 agagggagcc tcgcggtgat cgtggtgcct tcgccggagg tgtcgatctc gcacactccg
     7261 cccagttcgg cggcgcgttc gcgcatggag gacaggccca cccccggcac cgcgtcgagg
     7321 ggcatgccct ggccgttgtc gcggatctcc aggaccaggt cgacgccccg ggtgatggtg
     7381 atcgccgcgt gggtcgcggc ggcgtggcgg tggatgttgg tgagtgcctc ctgggcgatg
     7441 cggtaggccg ccacctcgac cgccgccggg aggccggtca ggtcgccgtc gaccgtcagg
     7501 gcgtcggggc cgagatcctt gacggcgccg gccaggccca ggtcgtcgag ggcgggtggg
     7561 cgcaggccgt agacgagttc gcggatgtcg ccggagacgg cgtccatgcc gcagcggagg
     7621 tcggccagca gggtgtcggt gatctcgggg ctgcccttga ggttcaggcg ggctgtggtg
     7681 atgcgcatcg ccatcgcggt cagggtctgg ccgaggccgt cgtgcaggtc gcggcgcagg
     7741 cgccggcgtt cctcctcgcg ggcggtcagg atgcgctcgc gggacttccg cagcgccgtg
     7801 gtcaggcgca tggtgtgggc ggcgtcggcg atgtacgggg tcagggccgc gatcacccgc
     7861 tcgtcgtggg cggcggggaa gcggcggcgg tccggcgggc cgatcaggag ggtgccgacc
     7921 ggttcgccgt gccagaccag gtcgacccgg cgggccacca cgggcagttc gccccagtcg
     7981 gtgcgggtgc cgtcgaggtc gagcgccgcg ccggtcaccg ccaggccctc tctgatcgtc
     8041 tccagggtgg cgagcagtcc gtggacgggg tcggtccgct ggagccgccg gcgcaggccg
     8101 tcggcgaggg tgaccgggtc gccgccggtg ccgtagaggg cccggtcggc gagccggtgc
     8161 agggcccgtt tcatcgggtg gaagaacacg ccggcgccga gggccgcgac caggccgaag
     8221 tagcggtcga cctcggccag gaacaggctg gaggcggtgc cggccagcac gtacacggag
     8281 gcgaagacgg tgaccatccc ggcgcccacc agcgcgcggg tgatcagggt gtcgatgccg
     8341 aacagccggt agcgcagcac ggccagggtg atcgcggccg ggatcagcgg gatcgtgatc
     8401 gcggtgaccc accacaccga gttgccgatg atccacggca cgctcaggca ggcgatggcc
     8461 agcagcggcc acaggatctg ccgccgttcg tcgccctcgg cccgccggaa ccggatcacc
     8521 agcgacgaga acgcgatcac cagcgagacc gtgacgaggt gccgcaggat cccctcgctc
     8581 cacggcgcgt agggcgcgaa cgcggggatc gcgagagatt tcggtccgat gatgtcgacg
     8641 atccagtcgg cggccagcac ggcggccgcg aagccgcccg cccagcacca cttccgcgac
     8701 aggagcgccc cggtcgggta gagcatcggc agcagcacgc ccagcgcgta ggtcgtcagg
     8761 ttccacacgc cgtgggagag gaccgcgagc ggcgtggtca gggtgtcgcg gtcgagcagc
     8821 agcgccaggt ccatcgagac gatgttggcc gccgcgccga gcccgcccag cgtcagcagc
     8881 cagccgatca ccagcttcgg ccgccgctgg accagcagcg ccccgaagac cgggaaggcg
     8941 acgcccatga cgtcttccgg cgtgaccggc agcggccact cgagctcggg cagccgggag
     9001 ctgatcacca ggtcggcgac gaccaggagg atcgccgccc ccgcgatccc cacggccagg
     9061 ccccgcgccg cccgatcggg cacccgcatc catttcggcc gggtctccac ctcgtgagag
     9121 gcaacggtca tcaggccccc gccttcgtct cgttcccgtg cgtctctgag acggggcccg
     9181 gtgagggaaa gttcagcaga tctccggcat ttcgggtgac ggtttgtcag aaggagtggt
     9241 gatggtccgc accttgggag gaggcgtgcc gtagaggcgt tcgccgaacc gctgggtcgc
     9301 gcccggccgt agccggaccc cggtgacggt ggcctggccg ccgaagacgt cagaaaccct
     9361 catcgtgtac ggccctggcc ccggccccgg cgggaacagc cagtagttgt cgggcccccg
     9421 gcgcagatcc cgccagtggg ctccgctcct gacccgcacg tgcttcagcc ggttgccgtg
     9481 gtcgagcacc tgcaccgcgc cccagtgggc ggtcgagccg ggcttcaccc ggaacgccag
     9541 ccgccgcccc ggctccgggt cgcggacctg ccggtaggtg atcgcggcgc tgccgtcgct
     9601 cttgtgggcg atgctgacga acgcctccgc cgacaggtcg agatcgccgg gaccgcaggc
     9661 cgggcagcgg tcgacgacca gcacccggac cgtcccggtc ggccccgtca cgtcgagata
     9721 gccgccgcag gcggccgacc cggcgtattc ggccgtcgac accgaggcga acagcatggg
     9781 atggcccggc agcgagcagt tccccggcgc cttcgcctcg tagaagtagg cccgccctcg
     9841 ctgggggccc ggggaggcgc agacgggatc ggtcagccgg acgaacaccg ccgccgcgac
     9901 cagcgccccg acggcgaacc ccactccgcg gcgccgccgg cgcctcgcgt gtctcatgtc
     9961 ggatacatag agacacggag cgattacgtt acgtagatct tcactggtag cgcggggact
    10021 ccagcagcca ttcgaggtag gccctcggca ctccgaacag acttgaggcc accttctcga
    10081 cctcgtcggg gaccccgagt tccgcgtacg cctccggcag cgccttcgcc gcgtcggtca
    10141 gatcctcggc ccgctcgacg atccggtcgg ccacccactc ggccggggcg ggcaccccga
    10201 tgcgcgacgc cgccacccgc aggtagttgt gcgggtcgcc gcgctccttg ccgagcgagg
    10261 ccacgtcgtt gcaccaggcc gtgacgtcga ccacggcgtc gaggagcgtc cgccacgggg
    10321 gcgtggcgtg cagccgcccg gggacctcga tccgcaggca cccctcgatg atctcggccc
    10381 cggcccccgc gaagacgtcc cgccgcagcg ccggatactc cccggccgac ggcacccggc
    10441 cgatcgaccg gttcaccgcc tgcatccggc aggcctgcgc ctgcgcctcc atccgcgccg
    10501 tgaaccgctg ctcccaggcc ggcgacatgt cgggcgccgt cacccgccac aggtcggcga
    10561 acgccgccac cagcggctcc tcggtccggt gaccggcgtg ggcggcgtcc cgcaggcggc
    10621 cctgcatcag gtcgagcacg gtggtgtccc ggctgccgct gccctcgtcg caccagtcgt
    10681 cgtaggccag ttgccaggtg acccaccggg cgagcagctc gttgcccggc agcaccctgg
    10741 cggccagacg ctcggggcgg gcggcgatcg agaggccgta cagaccggcc caggccccca
    10801 gcgcgcgcgt gtcgcactcg accggctctc cggcggtcgg cggcagagcg tagacgagcg
    10861 gggccagaac ggcagcagcc gaaggtatgc gcagctcagg ggtcatgtga tgacacgcta
    10921 ggtgagcatt caccgggtgt cccgcaggcg cgacggggag tcctcacggc aggtgcgtaa
    10981 cgtatgtgaa acacacaagg ggcacactgg tagatgccca gacctggaac tttcgtgagg
    11041 tagtcgcctt ggaccgccag caggaattcg ttctccgcac acttgaagag cgcgacatcc
    11101 ggttcatccg gctctggttc accgacgtcc tgggcttcct gaagtccgtg gccatcgcgc
    11161 cggccgaact cgagggcgcc ttcgccgagg gaatcggctt cgacggctcc gccatcgaag
    11221 gattctcccg ggtgtacgag tcggacatgc tcgccaagcc cgacccgtcg accttccaga
    11281 tcctgccgtg gcgcagcgag acccccgggg cggcgaggat gttctgcgac atcctgatgc
    11341 ccgacggatc tccgtcccac gccgaccccc gctgggtgct gaagcggacc ctggcgcgcg
    11401 ccgccgacat ggggttcacc ttctacaccc accccgaggt ggagttcttc ctcctccgca
    11461 accgtcccga gcgcggcgtc aagcccgagc cgatcgacga cggcggctac ttcgaccaca
    11521 ccccgcacag cgccggccac gacttccgcc gcaacgcgat catgatgctc gaggcgatgg
    11581 ggatctcggt cgagttcagc caccacgagg gcgctccggg gcagcaggag atagacctcc
    11641 ggtatgccga cgccctgtcg acggccgaca acatcatgac gttccgtctc gtgatgaagg
    11701 aggtggcgct cgaacagggc gtctgggcct ccttcatgcc gaagcccttc accgaatacc
    11761 ccgggtcggg gatgcacacc cacatgtccc tgttcgaggg ggacaggaac gccttctacg
    11821 agcccgggtc ggactaccag ctctccaagg tgggccgctc cttcatcggt ggcctcctca
    11881 agcacgcggc cgagatcacg gcggtgtgta accagtgggt gaactcttac aagcggttgt
    11941 ggggcgacac cgaggccatc gccggcgcgg gtggcgaggc tccctcgtac atctgctggg
    12001 ggcacaacaa ccggtcggcg ctggtccggg tgccgatgta caagccgcac aagggcgggt
    12061 cgacgcggat cgagttccgg tccctggatt cggcgtgcaa cccctacctg gcttacgcga
    12121 tgatcctcgc cgcggggctc aaggggatcg aagaggggta cgacatcccg gcaggggccg
    12181 aggatgacgt gtgggcactc acctctgggg agcggcgggc gctggggatc cacccgctgc
    12241 cgcagtcgct cgacgaggcg atcgtggcca tggagcacag cgaactcgcg gcggagaccc
    12301 tcggggagca cgtgttcgac tacttcctgc ggaacaagcg gtcggagtgg cgtgagtacc
    12361 gccgccaggt gaccgagttc gagctgggac gctacctgcc ggtcctgtaa agccaggtca
    12421 gagcgtaggg cgggacaacg atgagttgtt ccgccctgcg ctcttttaag agctctgggc
    12481 cgtcgggctg ggattcggca gcctcatggc ccttgtccac agcccgctgg gcatgaggtg
    12541 ggtgtagacc ctgagcgtga accctggatc agtgtggccg aggtgcctcg gcgcatgcat
    12601 gccgtgctcg cgcgcggcct gatgccctct tacccttccc cgactccggg atgacgtcgg
    12661 cggccacgag agcgggtttc caggcgtaaa tgtggaagtc gctccttctc accgggccat
    12721 tgcccgtgcg ggagaagagc aacagctctc aaaatgatca cactaggtga tcgccaatct
    12781 agcgaagtac tactagggaa atcccggatg cggtacctgg ggcgggcaag gtttgttcgt
    12841 gatgcagtgg tgttgcactg cggcgaaggc cgctggacga gaaggcatgt ttctgacgct
    12901 gcgtcgttct ggctggggat ttcccgtgat gaatgctggc ctgctcggct agaaagctta
    12961 cgagcggaga tgaatccgca tgtcgacctc caacatgtgc ctatcggaga cggaattcca
    13021 cgtgcaggaa ggcggaggac atgatggaac gacggttctt tttggaagag aaagaacttc
    13081 accaaggtca agtgcattcg gacgtcgcca aagtgcagaa gtacctgact agagagcctt
    13141 aatggctgag gttatgagag gtgggggtcc aggccggtct tggcgatgaa cccgctgagg
    13201 atgcggcggc ggtattgcat gcgtttgagc cggctcttca ccagcgcgag gagttggtcc
    13261 tggttgcggg tggccaggtt ggccagggat ttcttcatgt gggcccagac cgcttcgacg
    13321 gggttgaact ccggcgcgta ggcgggcagg tagaagaccg tcagccacgg gcgggccgcg
    13381 atcagctcgc gcatccgtgc tgagtggtgg gtgttgaggt tgtcccacac caccaccagt
    13441 ggcgcgccga gttgttgatg cgccgcgtcg aacagggccg cgtagtcggc ggctgcgaac
    13501 cctttcggct cccctttgcg tccgcgatag gggcgggtgc ggtagatcag cctggctcca
    13561 gcggccgggt cagccggttt gaccgcaacc agcgcggcga tggagatccg gccgccgcct
    13621 ttggcggtca ccgccaccac cggggtgatc ccgcgtcgtc cccaggtccg gcccttgggt
    13681 gatctcaagg tctgggccgc ctcgtcttcg aagcagagcc aggc
//
