LOCUS       NZ_BBXD01000010        10801 bp    DNA     linear   CON 08-AUG-2024
DEFINITION  Herbidospora mongoliensis strain NBRC 105882, whole genome shotgun
            sequence.
ACCESSION   NZ_BBXD01000010
VERSION     NZ_BBXD01000010.1
KEYWORDS    WGS; HIGH_QUALITY_DRAFT; RefSeq.
SOURCE      Herbidospora mongoliensis
  ORGANISM  Herbidospora mongoliensis
            Bacteria; Actinomycetota; Actinomycetes; Streptosporangiales;
            Streptosporangiaceae; Herbidospora.
REFERENCE   1
  AUTHORS   Komaki,H., Ichikawa,N., Oguchi,A., Hamada,M., Tamura,T. and
            Fujita,N.
  TITLE     Genome-based analysis of non-ribosomal peptide synthetase and type-I
            polyketide synthase gene clusters in all type strains of the genus
            Herbidospora
  JOURNAL   BMC Res Notes 8, 548 (2015)
   PUBMED   26452464
  REMARK    DOI:10.1186/s13104-015-1526-9 Publication Status: Online-Only
REFERENCE   2  (bases 1 to 663229)
  AUTHORS   Hosoyama,A., Oguchi,A., Ichikawa,N., Kimura,A., Ohshita,R. and
            Fujita,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAY-2015) Contact:Director-General Department Of
            Biotechnology National Institute of Technology and Evaluation,
            Biological Resource Center (NBRC); 2-49-10 Nishihara, Shibuya-ku,
            Tokyo 151-0066, Japan URL :http://www.nite.go.jp/nbrc/index.html
COMMENT     ##Genome-Assembly-Data-START##
            Finishing Goal                    :: Noncontiguous Finished
            Current Finishing Status          :: High-Quality Draft
            Assembly Method                   :: newbler v. 2.6
            Genome Coverage                   :: 62x
            Sequencing Technology             :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_001570545.1-RS_2024_08_08
            Annotation Date                   :: 08/08/2024 02:40:32
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,280
            CDSs (total)                      :: 8,202
            Genes (coding)                    :: 8,113
            CDSs (with protein)               :: 8,113
            Genes (RNA)                       :: 78
            rRNAs                             :: 4, 6, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 4, 5 (5S, 16S)
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 21 of 89
            Pseudo Genes (incomplete)         :: 71 of 89
            Pseudo Genes (internal stop)      :: 4 of 89
            Pseudo Genes (multiple problems)  :: 7 of 89
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 18:23:16
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 427749
            Orig. end                         :: 438550
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            BBXD01000010.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10801
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="4"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10801
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10801
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:5801](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5801
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            complement(620..1156)
                     /locus_tag="AW272_RS10750"
     CDS             complement(620..1156)
                     /EC_number="3.6.-.-"
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /locus_tag="AW272_RS10750"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="NUDIX hydrolase"
                     /protein_id="WP_066363293.1"
                     /transl_table=11
                     /translation="MNFPAWYAALPVAVSAAGVLFTDEASRVLLVRTTYKEDWDIPGGV
                     IEVDGGETPAAAARRELFEELGLDRPVGDLLSIDVQPPRPNRRAFVAFLFDGGTLTADD
                     LAGIRFQDGEIAEVRFVALDELAGFTDPALARRIGATLRHPGSGPLFLSDGEPTGNDRG
                     HGPHDRPDRAERPVR"
     gene            complement(1186..1590)
                     /locus_tag="AW272_RS10755"
     CDS             complement(1186..1590)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF07366"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020546924.1"
                     /locus_tag="AW272_RS10755"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="WP_066363300.1"
                     /sec_met_domain="PF07366 (E-value: 2e-07, bitscore: 26.1,
                     seeds: 29, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNTREIALRFARTWQTGWATHDVGLLVSLYAPDIVHRSMPFREPH
                     RGLAALREYLTWSFSEEIVLNVKFGEPLVDGDTAAIEFSVRAEEGHLAGCVFVRFDENG
                     LAVETRDYWHMTDNPEYVQKSGVTDHPGYA"
     misc_feature    complement(1438..1440)
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            1614..2318
                     /locus_tag="AW272_RS10760"
     CDS             1614..2318
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012890269.1"
                     /locus_tag="AW272_RS10760"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="DUF72 domain-containing protein"
                     /protein_id="WP_066363303.1"
                     /transl_table=11
                     /translation="MLVGTSGWQYADWRGVLYPPGLPQKRWLEHYGEEFGTVENNGAFY
                     RLPSRETFQEWHDRTPDDFVMAVKVSRYLTHVRRLRDPKEPVDRLMRMAEGLGDKLGPI
                     LLQLPPNLRWEDGTLEACLRCFPTSVALAVEPRHESWWNDDTRRTLEKHHAALVWSDRR
                     GRRQGPLWQTTTWAYLRFHEGTSKRHPPRYGDRALTTWLTRLPEDSYVYFNNDHAGAAV
                     INARRFTALATK"
     gene            2324..2974
                     /locus_tag="AW272_RS10765"
     CDS             2324..2974
                     /EC_number="2.1.1.-"
                     /GO_function="GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /GO_function="GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019048519.1"
                     /locus_tag="AW272_RS10765"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="WP_066363304.1"
                     /transl_table=11
                     /translation="MRTTRLLGSAELDASPVVANIAMNRGRRLASYSRELGLNLAGLLP
                     GASWLDLCCGEGHALAEAAMLGADVTGVDLVGHFAVPPGKNVELVVAAVPGWRPARKFD
                     LVTAVHGLHYVGDKLKFLADVAGWLTDHGRFAASFDVASVHTESRRKLLATLRSQGFVY
                     DSRKKLISRTGPAAIELPYEYLGADDKAGPNYTGQPAVVSHYRVYVDCTNPDS"
     gene            complement(2935..3447)
                     /locus_tag="AW272_RS10770"
     CDS             complement(2935..3447)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017591938.1"
                     /locus_tag="AW272_RS10770"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="dihydrofolate reductase family protein"
                     /protein_id="WP_066363306.1"
                     /transl_table=11
                     /translation="MTFTAAAFIGTSLDGFIARPDGDLTWLTTRGEQAGDLGYTDFIRD
                     IDTVALGRVTYETVLGFGAWPYEGKQVAVLSTTLPASDERVTVYRSFDDLVAGLTPHSK
                     RVYVDGGRLIQTFLREGRLDELTVTRAPVLIGEGMPLFGSLHGDVALTHRLTVAYESGF
                     VQSTYTR"
     gene            complement(3444..3926)
                     /locus_tag="AW272_RS10775"
     CDS             complement(3444..3926)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /locus_tag="AW272_RS10775"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="WP_066363731.1"
                     /transl_table=11
                     /translation="MVRPRTRDDETILRAAGLVIARHGPAGVTLALVAAEVGLSPATLV
                     QRFGSKRGLLLAFARHAAASVTLPPGDVVDALVTLSAGATDPGNLAFFQLDLTDADFRR
                     HAADHARRLEEQIRDRIGDPVLARLILLTHNGSLVDAALTGVSRLRDDLRALLETR"
     misc_feature    complement(3945..3956)
                     /note="TFBS match to AfsR, Pleiotropic regulatory for
                     antibiotic production, confidence: medium, score: 18.76"
                     /tool="antismash"
     misc_feature    3951..3964
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 15.15"
                     /tool="antismash"
     gene            3994..5004
                     /locus_tag="AW272_RS10780"
     CDS             3994..5004
                     /GO_function="GO:0004601 - peroxidase activity [Evidence
                     IEA]"
                     /GO_function="GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017540968.1"
                     /locus_tag="AW272_RS10780"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="Dyp-type peroxidase"
                     /protein_id="WP_066363307.1"
                     /transl_table=11
                     /translation="MSPATPSGVEDYRSGVQAVLGSLSPAAIFIVATIKPTGHQAVRDL
                     GEDLAGLLRTVGFRSLDPGLSCVVGYGSQFWDRLYPGLPKPQGLHPFKEIRGAHFAPST
                     PGDLLIHIRANRQDLCFELAGLIMNRLRDAVASADEVHGFRYFDARDLLGFVDGTENPT
                     GNLALDSVLIGDEDPDYAGGSYVLVQKYVHDLITWNGLATEEQEKVIGRQKLSNVELDD
                     EVKPSNAHNALTVIEVDGKEVKILRDNMPFGNASQGEFGTYFIGYSRTSELLELMLTNM
                     FIGDPPGNYDRILDFSSALTGSLYFVPSGAFLEDPPEPVRSGRLIIGDLKGVPQT"
     gene            5001..5801
                     /locus_tag="AW272_RS10785"
     CDS             5001..5801
                     /GO_component="GO:0140737 - encapsulin nanocompartment
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: Linocin_M18"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017980848.1"
                     /locus_tag="AW272_RS10785"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="family 1 encapsulin nanocompartment shell
                     protein"
                     /protein_id="WP_066363314.1"
                     /sec_met_domain="Linocin_M18 (E-value: 1.6e-92, bitscore:
                     304.4, seeds: 61, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNNLHRELAPISDAAWAQIEEEATRTFKRHIAGRRVVDVHGPDGT
                     ELSAVSTGHTTPIQPPSPDVQARQREVSRLIELRRPFELDREAIDDVERGSGDSDWQPV
                     KDAAAALAIAEDHAVFNGYDAGRIYGVIPGSAHSPLVLPADVRAYPDAIAQALGILRMA
                     GVDGPYSVLLSADAYTGVAETADHGYPVLEHIQRLVSGDIVWAPAISGAVVLTTRGGDF
                     DLHLGQDVSIGYLSHTDATVTLYLQESFTFLPFTAEAAIHLTTP"
     misc_feature    5804..5818
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 16.86"
                     /tool="antismash"
     misc_feature    complement(5824..5840)
                     /note="TFBS match to ZuR_variant_2, Zinc-responsive
                     repressor, confidence: weak, score: 17.63"
                     /tool="antismash"
     gene            5890..6522
                     /locus_tag="AW272_RS10790"
     CDS             5890..6522
                     /GO_function="GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA]"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1008: response
                     regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /locus_tag="AW272_RS10790"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="response regulator transcription factor"
                     /protein_id="WP_066363316.1"
                     /transl_table=11
                     /translation="MRVFVVDEDRQTAADIEAALVKSGYSVTVGPDRTAVLGYDVVFLG
                     VGADDGAEACREIRRTSDVPIIVLSARDSGAERIRGLRAGADDYLVKPICMEELEARME
                     AVLRRTRGGKTDVLQAGDIQIDAKRYLVWRDGRNVPLTPKEFALLRRLVQTPGEVVTKQ
                     TLLLENWKDLSRSAARTLDVHIAHLRAKLGEPNVVETVRGVGYRLVP"
     misc_feature    6331..6333
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     misc_feature    6385..6387
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            6580..7230
                     /locus_tag="AW272_RS10795"
     CDS             6580..7230
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="AW272_RS10795"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="SRPBCC family protein"
                     /protein_id="WP_083958180.1"
                     /transl_table=11
                     /translation="MRKKPFRLLSAPLLALTMVLSVMSSPARAETDPVLALQWELARTT
                     YTLFESLNHPNPALLRAARSQSISVDIAAPRDEVFAIYSNFENHLGANPLLQRVVTHSD
                     TTTAGVRTINQTAVENTPLQPGLPATPINTHAQQRINAAGYFYEVDSWTLPNIMTHQTI
                     TFEDLGAAGTRVTENLTFEASVLLIDYTLSQGVMAHQGIQSVFKSRIEGGFYS"
     misc_feature    6604..6606
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            complement(7227..7961)
                     /locus_tag="AW272_RS10800"
     CDS             complement(7227..7961)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1136: GntR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020542072.1"
                     /locus_tag="AW272_RS10800"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="GntR family transcriptional regulator"
                     /protein_id="WP_066363318.1"
                     /transl_table=11
                     /translation="MHPRYARIADELRAAILGGEIPVGTPLPSETVLAARHKVARGTVR
                     QAVAVLTAEGLVGSRQGARRIVLGRERSQNFAVLHSFAQWARAAGHEVTGQVIDQRRRG
                     AAAAEAARLGLTTGDSVLDVLRLRSLDGTAVLLERTVYAGWLAPAVERLPADCDSVTQA
                     LYESRGLIFAYGEHLIDAVAAGTEDARLLGVRRGGPLLRQRRVTTTREGRPAEWSDDRY
                     RAGSVTFAIRNAAGALAVHPQD"
     misc_feature    complement(7581..7583)
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     misc_feature    complement(8108..8121)
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 14.65"
                     /tool="antismash"
     gene            8152..9324
                     /locus_tag="AW272_RS10805"
     CDS             8152..9324
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012893797.1"
                     /locus_tag="AW272_RS10805"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="WP_066363320.1"
                     /transl_table=11
                     /translation="MVSSRVRLTGLAAGLLIAVAACGSAPGTEATSTSPAASGGAAAVD
                     ARTATSAADFGGVDKLYEAAKAEGKLHVIALPPSWANYGEILTAFKAKYPGIEIEEETP
                     DASSADEINAVKTRKGQDRAPDVLDVGQSFAYSGAAEDLFAPYKVATWDKIPAAQKEAN
                     GLWVNDYGGYVSIACDAKKIAACPQTFADLLKPEYKGKVAMNGNPTKAGSAFAGVYAAA
                     LANGGSFDDIQPGIDFFKKLKDAGNFNPVETTPATIEKGETQISIDWDYNNAAYAPIMA
                     AKGLDWKTVIPTDGKYISLYAQAINKDAPHPAAARLWQEFLYSVEGQNLYLKGFARPVL
                     LPAMIADGSVDQAASAALPPVDGEPSFPTADQVTKANADVASGWGAAVAG"
     gene            9324..10172
                     /locus_tag="AW272_RS10810"
     CDS             9324..10172
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1067: putative ABC
                     transporter permease protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012893798.1"
                     /locus_tag="AW272_RS10810"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="ABC transporter permease subunit"
                     /protein_id="WP_066363321.1"
                     /transl_table=11
                     /translation="MSALVKNRRRGGGAGWLAAAPLLAVTLIAFGIPVAVLVIGAFSVE
                     DGGLGFDNLSATMREPYIGSLWSSVKLSGVVAVLGAILGAFLAQAVLTSRSGLLREAVL
                     TASGVLSNFGGVPLAFIWIATLGNSGVVTAALGLGAGALYNFWGLALVYLYFSVPLMVL
                     VIAPALDGLKPQWREAAQNSGASTWQFWRMVGIPVLGPSLLGGGVLLFGGAFAAYATAA
                     AMVGASVPLVTLQIADALTGNVLIGYENVALALSLDMIVIALLVMAVYLPLQKRSSRWL
                     A"
ORIGIN
        1 gatctccagg ccgtcggacc cccggttgag cagggccagg aagaggtcgg ccgcgcgccc
       61 ccgcaccgcc accgtgccct tcccgtgccc cggctcccac acgacccgtc cctcttcgcc
      121 gtggatcatc cactcgcccg gcacgtcggt ggtgtgcagg tggacggtca cccccgggtc
      181 gagggtctga cgggaggtcg cgaccgtgtc gatccactcc tcgatgccgt ccacggccag
      241 gtcggcggcc agttcgtagg gctgcccgag cgccagcgcc gcgtcggccc ggtggacggt
      301 cgcctcgtgc agacgccgcc ggacccacca ctgcgccggg cgcgggccga ggaacgtcca
      361 cacctgcgtc gacgcgccga ccgattcgat cgcgtcctgg agaacctgcg ggccgccgtg
      421 cagccaggca agtgcgccct cggtgtcggc cgggggcttg ccgtcgcgga cctcgcgcgg
      481 gtccaggacg ccctcgatgc ggtcggagat gatctgggcc gaccagcggt cgccccggcc
      541 gacgtggcgg aagagttgct tcagggtcca gccggggcag gtcggcacgg gagtgtcggg
      601 gtcggcggtg tggaggagtt cagcgaaccg ggcgttctgc tcgatcaggg cggtcatgtg
      661 gtccatggcc acgatcattc ccggtcggct cgccatccga caggaacagc gggccggaac
      721 ccgggtgccg cagggtggcg ccgatccggc gggccagcgc cgggtcggtg aacccggcca
      781 gctcgtcgag cgcgacgaac cggacctcgg ctatctcacc gtcctggaac cggatcccgg
      841 ccaggtcgtc ggcggtcagc gtgcccccgt cgaagaggaa ggccacgaac gcgcgcctgt
      901 tcggccgcgg cggctgaaca tcgatgctga gcaggtcacc caccgggcgg tcgaggccca
      961 gctcctcgaa gagctcccgg cgggccgccg ccgcgggggt ctccccgccg tcgacctcga
     1021 tcacgccgcc ggggatgtcc cagtcttctt tgtacgtcgt ccgcaccagc agcacccggc
     1081 tcgcttcgtc ggtgaacagc actccggccg cggagacggc gacggggagg gcggcgtacc
     1141 aggcgggaaa attcaccggc cgactctaca accgccggcg gacgttcagg cgtagcccgg
     1201 gtggtctgtc acacctgatt tctggacata ttcgggatta tccgtcatat gccaatagtc
     1261 gcgggtctcg accgccagac cgttctcgtc gaaccgcacg aaaacacatc cggccaggtg
     1321 gccttcctcg gcgcgtacgg agaactcgat cgcggctgtg tccccgtcga ccaaaggctc
     1381 gccgaacttc acgttcagca cgatctcctc tgagaacgac cacgtcagat actcccgtaa
     1441 agccgcgagt ccccgatgcg gctcacggaa cggcatcgag cggtggacga tgtccggggc
     1501 gtacagagaa acaagcaggc cgacgtcgtg agtggcccag ccggtttgcc aggtgcgggc
     1561 gaagcggagg gcgatctccc tggtgttcac ccgccgaagc ttaggctggc gcaatgttgg
     1621 tgggaacgtc cggctggcag tacgccgact ggcgcggggt cctctatccg cccggcctgc
     1681 cgcagaaacg atggctggaa cactacggag aagaattcgg caccgtcgag aacaacggcg
     1741 ccttctaccg cctgccgagc cgcgaaacct ttcaggaatg gcacgacaga acgcctgacg
     1801 atttcgtcat ggcggtcaag gtgagtagat atctcaccca cgtcagacgc ctgcgcgacc
     1861 cgaaagaacc ggtcgaccgc ctgatgcgga tggccgaagg cctgggcgac aaactcggcc
     1921 cgatcctgct gcaactcccg cccaatctcc ggtgggaaga tggcaccctc gaagcctgcc
     1981 tcagatgctt ccccacaagc gtcgccctgg ccgtcgaacc ccgccacgaa tcgtggtgga
     2041 acgacgacac ccggcgaacc ctcgaaaagc accacgccgc cctcgtctgg agcgaccgcc
     2101 gtggccgcag gcaaggaccg ctgtggcaga ccacgacgtg ggcctacctg cgctttcacg
     2161 agggcacgtc gaagagacac ccaccccgat acggcgaccg agcgctcacc acgtggctga
     2221 ccagactgcc cgaagacagc tacgtctact tcaacaacga ccacgcgggg gccgcggtga
     2281 taaatgcgcg ccgcttcaca gcattggcga caaaataggg cgcatgcgca cgacgaggct
     2341 gttggggtcc gccgagctgg acgcgtctcc cgtcgtggcg aacatcgcga tgaaccgggg
     2401 acgccgtctg gcgagttact cccgtgagct cggcctgaac ctggccgggc tcctcccggg
     2461 cgcgtcctgg ctcgacctgt gctgtggcga gggccacgcg ctcgcggagg ccgcgatgct
     2521 cggtgccgat gtcacggggg tcgatctggt cggccacttc gccgtcccgc cggggaagaa
     2581 cgtggagctg gtcgtcgccg ccgtcccggg ctggcggccg gcccggaagt tcgacctggt
     2641 cactgccgtt cacggccttc actacgtcgg tgacaagctc aaattcctcg ccgacgtcgc
     2701 cgggtggctg accgatcacg gcaggttcgc ggccagtttc gacgtcgcct ccgtccacac
     2761 ggagtcgcgg cggaaactgc tggccacgtt gaggagccag gggttcgtct acgactccag
     2821 gaagaagctc atctccagaa ccggccccgc cgcgatcgag ctcccctacg aatacctggg
     2881 cgccgatgac aaggccgggc ccaactacac cggccagccg gccgtcgtct cccactatcg
     2941 ggtgtacgtc gactgcacga accccgactc gtaggccacc gtcagccgat gagtgagcgc
     3001 gacgtccccg tgcagggacc cgaaaagggg catcccctcg ccgatgagga ccggcgcgcg
     3061 ggtcacggtg agctcgtcga ggcggccctc ccgcaggaag gtctggatca ggcggcctcc
     3121 gtcgacgtac acccgcttgc tgtgcggagt cagcccggcc accaggtcgt cgaagctccg
     3181 gtagacggtc acccgctcat cggaggcggg cagcgtcgtg ctcaggaccg ctacctgctt
     3241 gccctcgtag ggccaggcgc cgaagccgag gaccgtctcg taggtcaccc ggccgagggc
     3301 gacggtgtcg atgtcgcgga tgaagtcggt gtagccgagg tcgccggcct gctcgccccg
     3361 ggtggtcagc caggtcagat cgccgtcggg gcgggcgatg aagccgtcga ggctggtgcc
     3421 gatgaaggcc gcggcggtga aggtcatctc gtctccaaga gtgcgcgcag gtcgtctcgc
     3481 agccgggaca ccccggtcag ggcggcgtcg acgaggctgc cgttgtgggt cagcaaaatc
     3541 agccgggcca ggacggggtc gccgatccgg tcgcggatct gctcctccag gcgccgggca
     3601 tggtcggcgg cgtggcgccg gaaatcggcg tcggtcaggt cgagctggaa gaacgccagg
     3661 ttcccggggt cggtggcgcc cgcgctcagg gtgacgagcg cgtcgactac gtcgccgggc
     3721 ggcagggtca ccgaggcggc ggcgtgccgg gcgaaggcca gcagcaggcc gcgtttcgag
     3781 ccgaagcgct gcaccagcgt ggccggcgac aggcccacct cggcggccac cagcgcgagc
     3841 gtcaccccgg cggggccgtg ccgggcgatc accaggccgg cggcccgcag gatcgtctcg
     3901 tcatctcgtg tacgcggtct caccacaccc accaatataa atgaccgttc atttatgaac
     3961 aaacccgtga cccgataagg agattgatca accatgtcac cggcgacccc tagcggggtg
     4021 gaggactatc gcagcggtgt gcaggcggtc ctgggctcgc tgagccctgc ggccatcttc
     4081 atcgtcgcca cgatcaagcc gaccggccat caggcggtca gggacctggg cgaagacctg
     4141 gccgggctgc tgcggacggt cggtttccgc tcgctcgacc cggggctgtc gtgtgtcgtc
     4201 ggctacggct cgcagttctg ggaccggctc tatccggggc tgcccaagcc gcaggggctg
     4261 cacccgttca aggagatcag gggcgcccac ttcgcgccct cgaccccggg cgacctgctg
     4321 atccacatca gggccaaccg gcaggacctc tgcttcgagc tggccgggct catcatgaac
     4381 cgcctcaggg acgccgtcgc cagcgcagac gaggtccacg gcttccgcta cttcgacgcc
     4441 cgcgacctgc tcggattcgt cgacggcacc gagaacccca ccgggaacct ggctctcgac
     4501 tcggtgctca tcggggacga ggaccccgac tacgccggcg gctcctacgt cctggtccag
     4561 aaatacgtcc acgacctgat cacctggaac ggcctcgcca ccgaggagca ggagaaggtc
     4621 atcggacgtc agaagctctc caacgtcgaa ctcgacgacg aggtgaagcc gtcgaacgcg
     4681 cacaacgcgc tcacggtcat cgaggtggac ggcaaggagg tgaagatcct ccgcgacaac
     4741 atgccgttcg gcaacgcctc gcagggggag ttcggcacct acttcatcgg ctactcgcgc
     4801 acctctgagc tgctggaact catgctgacc aacatgttca tcggcgaccc gccgggcaac
     4861 tacgaccgga tcctcgactt ctcctcggcg ctgaccggtt ccctctattt cgtcccctca
     4921 ggcgctttcc tggaagaccc gccggagccc gtgcggtccg gtcggttgat catcggcgac
     4981 ctcaagggag tcccgcagac atgaacaacc tccatcggga gctggccccg atctccgacg
     5041 ccgcctgggc gcagatcgag gaggaggcca cccgcacctt caagcgccac atcgccggcc
     5101 ggcgggtggt cgacgtccac ggccccgatg ggaccgaact gtcggcggtg tcgaccgggc
     5161 acaccacccc gatccagccg ccgtcgcccg acgtccaggc gcggcagcgg gaggtgtcgc
     5221 ggctgatcga gctgcgccgg cccttcgagc tcgaccgcga ggcgatcgac gacgtcgagc
     5281 gcggctcggg cgactccgac tggcagcccg tcaaggacgc cgccgccgcg ctggccatcg
     5341 ccgaggacca cgccgttttc aacggctacg acgccggccg gatctacggc gtcatcccgg
     5401 gcagcgcgca ctcgccgctg gtgctcccgg ccgatgtcag ggcctatccg gacgccatcg
     5461 cgcaggccct cggcatcctg cggatggcgg gggtcgacgg gccctattcg gtgctcctga
     5521 gcgccgacgc ctacacgggg gtggccgaga cggccgacca cggatatccg gtgctggagc
     5581 acatccagcg actggtctcc ggcgacatcg tctgggctcc ggccatctcc ggcgcggtcg
     5641 tgctcaccac ccggggtggc gacttcgatc tgcacctcgg gcaggacgtc tccattggtt
     5701 atctgagcca caccgacgcg acggtgacgc tgtatctcca ggagtcgttc acgttcctgc
     5761 cgttcaccgc ggaagcggcc attcatttga ccacacccta attatctttc agcaattgtt
     5821 gacatgtcat cctgtaccca ccagtattgc ccggacgagg cgggggccac agctttaaca
     5881 cggggagaca tgcgggtatt cgtcgtggac gaagataggc agacggcggc ggacatcgaa
     5941 gccgcactgg tgaaatccgg atattcggtc acggtcggtc ccgaccggac ggccgtgctc
     6001 ggatatgacg ttgtttttct cggcgtggga gccgacgacg gggcagaggc gtgccgggag
     6061 atccggcgga ccagcgacgt gccgatcatc gtgctttcgg cccgcgacag cggggccgaa
     6121 cggatccgcg gactgcgggc cggcgccgac gactatctcg tcaaacccat ttgcatggag
     6181 gaattggaag cgcggatgga agccgtcctg cggcggacgc ggggcggcaa gaccgacgtt
     6241 ctccaggccg gtgacatcca gatcgacgcg aaacgttatc tggtctggcg ggacggccgg
     6301 aatgtgccgc tgacgccgaa ggagttcgcg ttactccggc ggctcgtgca gacgccgggc
     6361 gaggtggtca ccaaacagac gctgttactc gagaactgga aggacctgag caggtcggcg
     6421 gcgcggactc tcgacgtcca catcgcgcac ctgcgggcca aactcggcga gccgaatgtg
     6481 gtggagaccg tgcggggagt gggctaccgg ttggtcccct gaaaagactg tcaggtgttg
     6541 ccaggagcgt tccaagggca catggggtga gatgtgccca tgagaaagaa gccgtttcgg
     6601 ctgttaagcg ctccactgct cgccctcacg atggtgctgt ccgtcatgag ctcgcccgcc
     6661 agggcggaga ccgacccggt gctggccctg cagtgggagc tggcccgcac cacgtacacc
     6721 ctgttcgaga gcctgaacca cccgaacccg gccctcctcc gggcggccag atcgcagagc
     6781 atctcggtcg acatcgcggc cccccgcgac gaggtgttcg cgatctactc gaacttcgag
     6841 aaccacctcg gcgccaaccc gctgctccag cgggtggtca cccactccga caccaccacc
     6901 gccggggtcc ggaccatcaa ccagaccgcg gtcgagaaca cgccgctcca gccggggctg
     6961 cccgcgaccc cgatcaacac ccacgcccag cagcggatca acgcggccgg atacttctac
     7021 gaggtcgact cgtggacgct gcccaacatc atgacccacc agacgatcac cttcgaggac
     7081 ctgggcgcgg ccgggacccg ggtgaccgag aacctgacgt tcgaggccag cgtcctgctg
     7141 atcgactaca cgctctccca gggcgtcatg gcccaccagg gcatccagtc ggtgttcaag
     7201 agccgcatcg agggcgggtt ctacagctag tcctgcggat gcacggccag ggcgcccgcc
     7261 gcgttgcgga tcgcgaacgt cacgctgccg gctcggtagc ggtcgtccga ccactcggct
     7321 gggcgccctt cccgtgtcgt cgtcacccgc cgctgccgca gcagcggccc gccgcgccgc
     7381 actccgagca ggcgggcgtc ctcggtcccg gccgcgacgg cgtcgatgag gtgctctccg
     7441 tacgcgaaga tcaggcccct ggactcgtac agggcctggg tgaccgagtc gcagtctgcg
     7501 ggcagccgtt cgacggcggg ggcgagccag cccgcgtaca cggtccgttc cagcaggacc
     7561 gccgtgccgt cgaggctgcg taaccggagc acgtcgagca ccgagtcacc ggtggtcagc
     7621 ccgaggcggg ccgcctcggc ggcggcggcc ccgcggcggc gctggtcgat cacctggccg
     7681 gtcacctcgt gcccggcggc ccgcgcccac tgggcgaaac tgtgcagcac cgcgaagttc
     7741 tggctgcgtt cgcggccgag aacgatccgc ctggcaccct ggcgggagcc cacgagccct
     7801 tcggccgtca gcaccgcgac ggcctggcgg accgttcccc gggcgacctt gtgccgggcg
     7861 gccagcacgg tctccgaggg cagcggtgtg cccaccggga tctcaccgcc gaggatggcc
     7921 gcccggagct cgtcggcgat gcgcgcgtat cgcgggtgca cggcgactcc cccaactttg
     7981 ctgtgtacag ggcaggcttc accgtacggt ccggaaatcg tccggaaaag tgggggttta
     8041 gccatttgac gaatggccgc cgactgttca ccgtcaggac gtgggtggtc gatctcgtgt
     8101 ccctagcttg tttgaacaag ttaaggcact tcgctccagt gggagttcat tgtggtctct
     8161 tcccgagtcc gtctcaccgg cctcgcagcc ggtctgctga tcgccgtcgc ggcctgtggg
     8221 tccgcgcccg gcaccgaggc aacctctact tctccggccg cctctggcgg cgccgccgcg
     8281 gtcgacgcca ggaccgcgac ctcggccgcc gacttcggcg gtgtcgacaa gctgtatgag
     8341 gccgccaagg ccgagggcaa gttgcacgtc atcgcgctgc cgccgagctg ggccaactac
     8401 ggcgagatcc tgaccgcctt caaggccaag taccccggca tcgagatcga ggaggagacc
     8461 cccgacgcct cctccgccga cgagatcaac gcggtgaaga cccgcaaggg ccaggaccgc
     8521 gcccccgacg tcctggacgt cggccagtcg tttgcctact ccggcgcggc cgaggacctg
     8581 ttcgccccgt acaaggtcgc gacctgggac aagatccccg ccgcgcagaa ggaggccaat
     8641 ggcctctggg tgaacgacta cggcggctac gtctccatcg cctgcgacgc caagaagatc
     8701 gcggcctgcc cgcagacctt cgccgacctg ctcaagcccg aatacaaggg caaggtcgcg
     8761 atgaacggca acccgaccaa ggcgggctcc gccttcgccg gcgtgtacgc cgccgcgctc
     8821 gccaacggcg gttcgttcga cgacatccag cccggcatcg acttcttcaa gaagctcaag
     8881 gacgccggta acttcaaccc ggtcgagacg accccggcga ccatcgagaa gggcgagacc
     8941 cagatctcga tcgactggga ctacaacaac gccgcctacg cgccgatcat ggccgcgaag
     9001 ggcctcgact ggaagaccgt catccccacc gacggcaagt acatctcgct ctacgcccag
     9061 gccatcaaca aggacgcccc ccacccggcc gccgcgcggc tgtggcagga gttcctctac
     9121 agcgtcgagg gccagaacct gtacctgaag ggcttcgccc gcccggtgct gctgcccgcg
     9181 atgatcgccg acggctcggt cgaccaggcc gcctccgccg ccctgccgcc ggtcgacggc
     9241 gagccgtcgt tcccgaccgc cgaccaggtc acgaaggcga acgcggacgt cgcctccggc
     9301 tggggcgccg cggtcgcggg ctgatgtctg ccctggtgaa gaaccggcgc cgcggtggcg
     9361 gcgccgggtg gctggcggcg gcgcctctgc tcgccgtcac gctgatcgcg ttcggcatcc
     9421 cggtggcggt gctggtcatc ggcgcgttct cggtcgagga cggcgggctc gggttcgaca
     9481 acctgtcggc gacgatgcgg gagccgtaca tcgggtcgct gtggagcagc gtcaagctgt
     9541 cgggcgtggt cgccgtcctg ggcgcgatcc tcggggcgtt cctcgcgcag gcggtgctga
     9601 cctcgcggtc cggactgctg cgggaggccg tgctgacggc gtccggggtg ctgtccaact
     9661 tcggcggcgt gccgctcgcg ttcatctgga tcgccacgct gggcaactcc ggcgtggtca
     9721 ccgccgccct cggcctcggc gcgggcgcgc tctacaactt ctggggcctg gccctggtct
     9781 acctctactt ctccgtaccg ctgatggtgc tggtcatcgc ccccgcgctc gacggcctca
     9841 agccgcagtg gcgcgaggcc gcgcagaaca gcggcgcctc gacctggcag ttctggcgga
     9901 tggtcggcat cccggtcctc ggcccgtcgc tgctcggcgg gggcgtgctg ctgttcggcg
     9961 gcgcgttcgc cgcctacgcg accgccgccg cgatggtcgg cgcgtcggtg ccgctggtga
    10021 cactccagat cgccgacgcc ctcacgggca acgtgctcat cgggtacgag aacgtcgccc
    10081 tcgcgctgtc cctcgacatg atcgtgatcg cgctgctggt gatggccgtc tacctgccgc
    10141 tgcagaagag gagctcccga tggctggcgt gacctggcgc cggatcactc tgggcgtggc
    10201 ggtggtctac ttcctggtgc cgctggccat cgcggtctgg ttcaccgtct acaacgagtc
    10261 gcagggcttc tccctcggcg cctacggccg gatcgtctcg gcccccggct tcggcgagag
    10321 cctgctgaag tcgctgctgc tcggggccgc ggtcatcgtg ctggtcctgc tcctggccct
    10381 acccgctctc ttggctgtac gactaggggc ccccggcctg cgcccggtgc tggagaccat
    10441 cacgacgctg ccgctgatcg tgccgccgat cacctacgtg gtcggcatcg ccaacgccct
    10501 gcgcggcggc accgacgccc tggcggccac cccgttctgg cagaccctca tctccatcca
    10561 ggacgaggac ttccccgtcg tcctggtcct ggcgtacgtc atgctcgcgc tgccgttcgc
    10621 ctaccgttcg ctggacaccg gcctgcgggc catcgacgtg cgcaccctcg tggaggcggc
    10681 ccggaacctg ggcgcctcgt ggccgtacgt gctgttccgg gtgatcctgc cgaacctgcg
    10741 ctcggccgtg gccagcgccg gcttcctcag cctggcgctc gtcatgggcg agtacacgat
    10801 c
//
