LOCUS       NZ_KQ948897             2983 bp    DNA     linear   CON 21-APR-2024
DEFINITION  Streptomyces bungoensis strain DSM 41781 PRJNA299233_s047, whole
            genome shotgun sequence.
ACCESSION   NZ_KQ948897
VERSION     NZ_KQ948897.1
KEYWORDS    WGS; RefSeq.
SOURCE      Streptomyces bungoensis
  ORGANISM  Streptomyces bungoensis
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 2983)
  AUTHORS   Ruckert,C., Winkler,A., Kalinowski,J., Kampfer,P. and Glaeser,S.
  TITLE     Draft genome sequence of Streptomyces bungoensis DSM 41781, type
            strain for the species Streptomyces bungoensis
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2983)
  AUTHORS   Ruckert,C., Winkler,A., Kalinowski,J., Kampfer,P. and Glaeser,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2015) CeBiTec, Bielefeld University, Sequenz 1,
            Bielefeld 33615, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 13-OCT-2014
            Assembly Method                   :: Newbler v. 2.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 60.6x
            Sequencing Technology             :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_001514215.1-RS_2024_04_21
            Annotation Date                   :: 04/21/2024 02:15:15
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,539
            CDSs (total)                      :: 7,454
            Genes (coding)                    :: 7,226
            CDSs (with protein)               :: 7,226
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 228
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 67 of 237
            Pseudo Genes (incomplete)         :: 196 of 237
            Pseudo Genes (internal stop)      :: 10 of 237
            Pseudo Genes (multiple problems)  :: 43 of 237
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 00:26:42
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 0
            Orig. end                         :: 2983
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            KQ948897.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     gene            <1..>2000
                     /locus_tag="AQJ66_RS36495"
                     /old_locus_tag="AQJ66_36495"
     source          1..2983
                     /country="Japan"
                     /culture_collection="DSM:41781"
                     /db_xref="taxon:285568"
                     /isolation_source="soil"
                     /mol_type="genomic DNA"
                     /organism="Streptomyces bungoensis"
                     /strain="DSM 41781"
                     /submitter_seqid="PRJNA299233_s047"
                     /type_material="type strain of Streptomyces bungoensis"
     region          1..2983
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="T1PKS"
                     /region_number="1"
                     /rules="cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or
                     hyb_KS or itr_KS or tra_KS))"
                     /tool="antismash"
     cand_cluster    1..2983
                     /SMILES=""
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="cds(PKS_AT and (PKS_KS or ene_KS or
                     mod_KS or hyb_KS or itr_KS or tra_KS))"
                     /kind="single"
                     /product="T1PKS"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..2983
                     /aStool="rule-based-clusters"
                     /category="PKS"
                     /contig_edge="True"
                     /core_location="[4:>2000](+)"
                     /cutoff="20000"
                     /detection_rule="cds(PKS_AT and (PKS_KS or ene_KS or mod_KS
                     or hyb_KS or itr_KS or tra_KS))"
                     /neighbourhood="20000"
                     /product="T1PKS"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5..>2000
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="cds(PKS_AT and (PKS_KS or ene_KS or mod_KS
                     or hyb_KS or itr_KS or tra_KS))"
                     /neighbourhood="20000"
                     /product="T1PKS"
                     /protocluster_number="1"
     CDS             5..>2000
                     /NRPS_PKS="Domain: PKS_KS(Modular-KS) (1-285). E-value:
                     2.3e-128. Score: 420.7. Matches aSDomain:
                     nrpspksdomains_AQJ66_RS36495_PKS_KS.1"
                     /NRPS_PKS="Domain: PKS_AT (384-665). E-value: 5.8e-109.
                     Score: 356.0. Matches aSDomain:
                     nrpspksdomains_AQJ66_RS36495_PKS_AT.1"
                     /codon_start=3
                     /gene_functions="biosynthetic (rule-based-clusters) T1PKS:
                     mod_KS"
                     /gene_functions="biosynthetic (rule-based-clusters) T1PKS:
                     PKS_AT"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) ketoacyl-synt"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1021: malonyl CoA-acyl carrier protein transacylase"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014048977.1"
                     /locus_tag="AQJ66_RS36495"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AQJ66_36495"
                     /product="type I polyketide synthase"
                     /protein_id="WP_159052714.1"
                     /sec_met_domain="mod_KS (E-value: 1.3e-158, bitscore:
                     515.4, seeds: 217, tool: rule-based-clusters)"
                     /sec_met_domain="ketoacyl-synt (E-value: 7.6e-43, bitscore:
                     133.9, seeds: 77, tool: rule-based-clusters)"
                     /sec_met_domain="PKS_AT (E-value: 1.9e-110, bitscore:
                     356.0, seeds: 1682, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTGTSGSVTSGRLAYFLGLEGPAVTVDTACSSSLVALHQAAQAVR
                     NGECALALAGGVTVMSSPGVLVEFSRQRGLSADGRCKAFAASADGTGWGEGAGLVVLER
                     LSAARARGHRVLAVVRGSAVNQDGASNGLTAPNGPSQQRVIRAALAAAGLTAADVDVVE
                     AHGTGTQLGDPIEAQALLATYGAEHSQEQPLWLGSLKSNVGHTMAAAGVGGVIKMVQAL
                     RRGVLPATLHVDEPTPHVDWDAGGVRLLTDSREWPSTGRPRRAGVSSFGISGTNAHVIL
                     EQAPSVEQEAPVEAGGPVGWVVSARSEEALRAYAAQLAAAVDGSGVSVGDVAATLAGRA
                     VFEHRGVVVGASREELVAGLRSLAEGVPAENVVAGTAGAGRTALVFPGQGSQWDGMARE
                     LYAAAPVFRARLDECAHALSAFVEWDLLEVLLTDEGASLLARVDVVQPALFAVMVSLAA
                     LWRSHGVRVDAVLGHSQGEIAAACVAGALSLEDAARVVALRSQAIRALAGRGGMVSVAL
                     PVERVRPRLTLWDGDIGIAAINGPSSTVVSGSAWALDELQAVFEADGVRTWRIPVDYAS
                     HSWHVDEIRDQVLELLAPIKPQAGDLRFYSTVTGQQIDTAELGADYWFRNLRGTVEFEA
                     VVRSLLADGFDTFIECSPHPVLAAGVQETID"
     aSDomain        10..861
                     /ASF="found active site cysteine: True, scaffold matched
                     GSSS: True"
                     /ASF="found active site histidines: True"
                     /aSDomain="PKS_KS"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_AQJ66_RS36495_PKS_KS.1"
                     /domain_subtypes="Modular-KS"
                     /evalue="2.30E-128"
                     /label="AQJ66_RS36495_PKS_KS.1"
                     /locus_tag="AQJ66_RS36495"
                     /protein_end="285"
                     /protein_start="1"
                     /score="420.7"
                     /tool="antismash"
                     /translation="TGTSGSVTSGRLAYFLGLEGPAVTVDTACSSSLVALHQAAQAVRN
                     GECALALAGGVTVMSSPGVLVEFSRQRGLSADGRCKAFAASADGTGWGEGAGLVVLERL
                     SAARARGHRVLAVVRGSAVNQDGASNGLTAPNGPSQQRVIRAALAAAGLTAADVDVVEA
                     HGTGTQLGDPIEAQALLATYGAEHSQEQPLWLGSLKSNVGHTMAAAGVGGVIKMVQALR
                     RGVLPATLHVDEPTPHVDWDAGGVRLLTDSREWPSTGRPRRAGVSSFGISGTNAHVILE
                     QAP"
     aSModule        10..>2000
                     /domains="nrpspksdomains_AQJ66_RS36495_PKS_KS.1"
                     /domains="nrpspksdomains_AQJ66_RS36495_PKS_AT.1"
                     /incomplete
                     /locus_tags="AQJ66_RS36495"
                     /tool="antismash"
                     /type="pks"
     CDS_motif       67..114
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_AQJ66_RS36495_0001"
                     /evalue="2.80E-07"
                     /label="PKSI-KS_m3"
                     /locus_tag="AQJ66_RS36495"
                     /protein_end="36"
                     /protein_start="20"
                     /score="22.3"
                     /tool="antismash"
                     /translation="GPAVTVDTACSSSLVA"
     CDS_motif       409..429
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_AQJ66_RS36495_0002"
                     /evalue="6.30E+01"
                     /label="PKSI-AT-M_m5"
                     /locus_tag="AQJ66_RS36495"
                     /protein_end="141"
                     /protein_start="134"
                     /score="-2.2"
                     /tool="antismash"
                     /translation="APNGPSQ"
     CDS_motif       490..534
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_AQJ66_RS36495_0003"
                     /evalue="4.60E-05"
                     /label="PKSI-KS_m5"
                     /locus_tag="AQJ66_RS36495"
                     /protein_end="176"
                     /protein_start="161"
                     /score="15.1"
                     /tool="antismash"
                     /translation="VEAHGTGTQLGDPIE"
     CDS_motif       595..639
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_AQJ66_RS36495_0004"
                     /evalue="3.20E-03"
                     /label="PKSI-KS_m6"
                     /locus_tag="AQJ66_RS36495"
                     /protein_end="211"
                     /protein_start="196"
                     /score="10.3"
                     /tool="antismash"
                     /translation="GSLKSNVGHTMAAAG"
     CDS_motif       895..918
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_AQJ66_RS36495_0005"
                     /evalue="4.80E+01"
                     /label="PKSI-AT-M_m5"
                     /locus_tag="AQJ66_RS36495"
                     /protein_end="304"
                     /protein_start="296"
                     /score="-1.9"
                     /tool="antismash"
                     /translation="GPVGWVVS"
     CDS_motif       1108..1131
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_AQJ66_RS36495_0006"
                     /evalue="7.00E+01"
                     /label="PKSI-AT-M_m5"
                     /locus_tag="AQJ66_RS36495"
                     /protein_end="375"
                     /protein_start="367"
                     /score="-2.4"
                     /tool="antismash"
                     /translation="PAENVVAG"
     CDS_motif       1156..1209
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_AQJ66_RS36495_0007"
                     /evalue="3.20E-09"
                     /label="PKSI-AT-mM_m1"
                     /locus_tag="AQJ66_RS36495"
                     /protein_end="401"
                     /protein_start="383"
                     /score="28.4"
                     /tool="antismash"
                     /translation="LVFPGQGSQWDGMARELY"
     aSDomain        1159..>2000
                     /ASF="Methylmalonyl-CoA specific"
                     /ASF="found active site serine: True, scaffold matched
                     GHGE: True"
                     /aSDomain="PKS_AT"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_AQJ66_RS36495_PKS_AT.1"
                     /evalue="5.80E-109"
                     /label="AQJ66_RS36495_PKS_AT.1"
                     /locus_tag="AQJ66_RS36495"
                     /protein_end="665"
                     /protein_start="384"
                     /score="356.0"
                     /specificity="substrate consensus: pk"
                     /specificity="PKS signature: Methylmalonyl-CoA"
                     /specificity="Minowa: emal"
                     /tool="antismash"
                     /translation="VFPGQGSQWDGMARELYAAAPVFRARLDECAHALSAFVEWDLLEV
                     LLTDEGASLLARVDVVQPALFAVMVSLAALWRSHGVRVDAVLGHSQGEIAAACVAGALS
                     LEDAARVVALRSQAIRALAGRGGMVSVALPVERVRPRLTLWDGDIGIAAINGPSSTVVS
                     GSAWALDELQAVFEADGVRTWRIPVDYASHSWHVDEIRDQVLELLAPIKPQAGDLRFYS
                     TVTGQQIDTAELGADYWFRNLRGTVEFEAVVRSLLADGFDTFIECSPHPVLAAGVQETI
                     "
     CDS_motif       1324..1392
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_AQJ66_RS36495_0008"
                     /evalue="4.90E-12"
                     /label="PKSI-AT-mM_m2"
                     /locus_tag="AQJ66_RS36495"
                     /protein_end="462"
                     /protein_start="439"
                     /score="37.5"
                     /tool="antismash"
                     /translation="ARVDVVQPALFAVMVSLAALWRS"
     CDS_motif       1396..1506
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_AQJ66_RS36495_0009"
                     /evalue="1.90E-18"
                     /label="PKSI-AT-mM_m3"
                     /locus_tag="AQJ66_RS36495"
                     /protein_end="500"
                     /protein_start="463"
                     /score="58.2"
                     /tool="antismash"
                     /translation="GVRVDAVLGHSQGEIAAACVAGALSLEDAARVVALRS"
     CDS_motif       1612..1650
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_AQJ66_RS36495_0010"
                     /evalue="5.20E-05"
                     /label="PKSI-AT-M_m5"
                     /locus_tag="AQJ66_RS36495"
                     /protein_end="548"
                     /protein_start="535"
                     /score="16.3"
                     /tool="antismash"
                     /translation="AAINGPSSTVVSG"
     CDS_motif       1720..1752
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_AQJ66_RS36495_0011"
                     /evalue="1.30E-03"
                     /label="PKSI-AT-mM_m6"
                     /locus_tag="AQJ66_RS36495"
                     /protein_end="582"
                     /protein_start="571"
                     /score="11.6"
                     /tool="antismash"
                     /translation="VDYASHSWHVD"
     CDS_motif       1858..1905
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_AQJ66_RS36495_0012"
                     /evalue="8.20E-05"
                     /label="PKSI-AT-mM_m8"
                     /locus_tag="AQJ66_RS36495"
                     /protein_end="633"
                     /protein_start="617"
                     /score="14.8"
                     /tool="antismash"
                     /translation="LGADYWFRNLRGTVEF"
     CDS_motif       1948..1977
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_AQJ66_RS36495_0013"
                     /evalue="1.20E-03"
                     /label="PKSI-AT-mM_m9"
                     /locus_tag="AQJ66_RS36495"
                     /protein_end="657"
                     /protein_start="647"
                     /score="11.3"
                     /tool="antismash"
                     /translation="FIECSPHPVL"
     assembly_gap    2001..2480
                     /estimated_length=480
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2489..>2983
                     /locus_tag="AQJ66_RS36500"
                     /old_locus_tag="AQJ66_36500"
     CDS             2489..>2983
                     /NRPS_PKS="Domain: PKS_DH (2-128). E-value: 4.1e-44. Score:
                     142.8. Matches aSDomain:
                     nrpspksdomains_AQJ66_RS36500_PKS_DH.1"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF026115.4"
                     /locus_tag="AQJ66_RS36500"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AQJ66_36500"
                     /product="polyketide synthase dehydratase domain-containing
                     protein"
                     /protein_id="WP_244163044.1"
                     /transl_table=11
                     /translation="MVGTVVFPGAAFVELAVHAADRVGCGSIRELTVQTPLVLPEHGAL
                     RLRVEVSEPTTDDGARPIRIDARPDTGTGASWTCHATGVLDVDEPSPDWDLTAWPPAGA
                     RPVDVSYDTLADHGYRYGPLFQGLQRMWRHNDHVYAEVALPTDPDAYTIHPALLDATLH
                     TA"
     CDS_motif       2495..2548
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_AQJ66_RS36500_0001"
                     /evalue="1.50E-06"
                     /label="PKSI-DH_m2"
                     /locus_tag="AQJ66_RS36500"
                     /protein_end="20"
                     /protein_start="2"
                     /score="20.8"
                     /tool="antismash"
                     /translation="GTVVFPGAAFVELAVHAA"
     aSDomain        2495..2872
                     /ASF="catalytic triad H,G,P inconclusive"
                     /aSDomain="PKS_DH"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_AQJ66_RS36500_PKS_DH.1"
                     /evalue="4.10E-44"
                     /label="AQJ66_RS36500_PKS_DH.1"
                     /locus_tag="AQJ66_RS36500"
                     /protein_end="128"
                     /protein_start="2"
                     /score="142.8"
                     /tool="antismash"
                     /translation="GTVVFPGAAFVELAVHAADRVGCGSIRELTVQTPLVLPEHGALRL
                     RVEVSEPTTDDGARPIRIDARPDTGTGASWTCHATGVLDVDEPSPDWDLTAWPPAGARP
                     VDVSYDTLADHGYRYGPLFQGL"
     CDS_motif       2840..2872
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_AQJ66_RS36500_0002"
                     /evalue="3.80E-03"
                     /label="PKSI-DH_m3"
                     /locus_tag="AQJ66_RS36500"
                     /protein_end="128"
                     /protein_start="117"
                     /score="10.2"
                     /tool="antismash"
                     /translation="GYRYGPLFQGL"
ORIGIN
        1 tgctgaccgg taccagtggg agtgtcacgt cgggccggtt ggcgtacttc ctcggtctgg
       61 aggggccggc ggtgacggtg gacacggcgt gttcgtcgtc gttggtggcg ttgcatcagg
      121 ccgctcaggc ggtgcggaac ggcgaatgcg ctctggcatt ggccggcggt gtgacggtga
      181 tgtcctcacc tggggtgctg gtggagttct cgcggcagcg cgggttgtcg gcggatgggc
      241 ggtgcaaggc gttcgcggcg tcggccgacg gtactggttg gggtgagggt gcggggctgg
      301 tggtgctgga gcgtctgtcg gctgcgcggg cgcgtgggca tcgggtgctg gcggtggtgc
      361 ggggttcggc ggtgaaccag gacggcgctt cgaatgggct gactgcgccg aacggtccgt
      421 cgcagcagcg ggtgatccgt gcggcgttgg cggctgccgg actcactgct gcggatgtcg
      481 acgtggtcga ggcgcacggc acgggtactc agctcggtga tccgatcgag gcgcaggcgt
      541 tgctggccac ctatggggcc gaacactccc aggagcagcc gttgtggctg ggttcgttga
      601 agtcgaatgt gggtcacacc atggcggcgg cgggggtcgg tggtgtgatc aagatggtgc
      661 aggcgctgcg tcgtggggtg ctgcctgcca cgttgcatgt ggatgagccg acgccgcatg
      721 tggactggga tgccgggggg gtgcggttgc tcaccgactc gcgtgagtgg ccttcgacgg
      781 ggcggccgcg tcgtgctggt gtgtcgtcgt tcggtatcag cggcaccaac gcgcatgtga
      841 tcttggagca ggcgccgtcg gtggagcagg aggcaccggt tgaggcgggg gggccggtgg
      901 ggtgggtggt gtcggcgcgg tcggaggagg cgttgcgggc gtatgcggcg cagctcgccg
      961 cggccgttga cggttccggg gtgtcggtgg gggatgtggc ggcgacgctg gccgggcgtg
     1021 cggtgttcga gcatcgtggt gtggtggtcg gtgcctcgcg tgaggagttg gtggcgggtc
     1081 tgcggtcgct ggcggagggc gttcctgctg agaacgtggt ggcggggacg gctggggccg
     1141 gcaggacggc gttggtgttt ccgggtcagg ggtcgcagtg ggatgggatg gctcgggagt
     1201 tgtacgcggc tgctccggtg ttccgcgccc gtctggatga atgtgcgcat gctctgtccg
     1261 cctttgtgga gtgggacctg ttggaggtct tgctgaccga tgagggtgcg tcgctgttgg
     1321 cgcgggtgga tgtggtgcag ccggcgttgt tcgcggtgat ggtgtcgttg gcggcgttgt
     1381 ggcgttcgca cggggtgcgg gtggatgcgg tgctggggca ttcgcaggga gagatcgcgg
     1441 cggcgtgtgt ggcgggggcg ttgtcgctgg aggacgcggc gcgtgtggtg gcgttgcgca
     1501 gtcaggcgat tcgggcgctg gccgggcggg gtggcatggt ttccgtggcg ttgccggtcg
     1561 agcgggtgcg gcctcgtttg acgctgtggg acggtgacat cgggatcgct gcgatcaatg
     1621 gtcccagttc tactgtggtg tcgggcagtg cgtgggctct ggatgagttg caggcggtgt
     1681 tcgaggccga tggtgtgcgc acctggcgca tcccggtcga ttacgcgtca cactcctggc
     1741 acgtcgatga gatccgtgat caggtgctgg agctgctggc gccgatcaaa ccgcaagccg
     1801 gcgacctgcg cttttactcc accgtcaccg ggcagcagat cgacaccgct gaactcggcg
     1861 cggattactg gttccgtaat ctccgtggga ccgtcgagtt cgaggccgtc gtccggtcgt
     1921 tgctggcgga cggttttgac acgttcatcg agtgcagtcc gcacccggtt ctggccgccg
     1981 gtgtgcagga gaccatcgac nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2461 nnnnnnnnnn nnnnnnnnnn atcacgaggt ggtcggcacg gttgtgttcc ccggtgccgc
     2521 gttcgtggag ctggcggtgc atgccgccga ccgggtcggc tgcggcagta tccgtgagct
     2581 gaccgtgcag acccccctcg tcctgcccga gcacggtgcc ctacggctgc gggtggaggt
     2641 gagcgagccc accaccgacg acggtgcgcg tccgattcgc attgacgcac gccccgacac
     2701 gggcacggga gcctcctgga cctgtcacgc cacgggtgtc ctggacgtcg atgagccttc
     2761 cccggactgg gacctcaccg cctggccgcc tgccggggca cggccggtgg acgtctccta
     2821 cgacaccctg gccgaccacg ggtaccgcta cgggccgctc ttccagggct tgcagcggat
     2881 gtggcgccac aacgaccacg tctacgccga ggtcgccctt cccaccgacc cggacgccta
     2941 caccatccac cccgctctcc tcgacgccac cctccacacc gcg
//
