LOCUS       NZ_KQ948859            10498 bp    DNA     linear   CON 21-APR-2024
DEFINITION  Streptomyces bungoensis strain DSM 41781 PRJNA299233_s009, whole
            genome shotgun sequence.
ACCESSION   NZ_KQ948859
VERSION     NZ_KQ948859.1
KEYWORDS    WGS; RefSeq.
SOURCE      Streptomyces bungoensis
  ORGANISM  Streptomyces bungoensis
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 278034)
  AUTHORS   Ruckert,C., Winkler,A., Kalinowski,J., Kampfer,P. and Glaeser,S.
  TITLE     Draft genome sequence of Streptomyces bungoensis DSM 41781, type
            strain for the species Streptomyces bungoensis
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 278034)
  AUTHORS   Ruckert,C., Winkler,A., Kalinowski,J., Kampfer,P. and Glaeser,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2015) CeBiTec, Bielefeld University, Sequenz 1,
            Bielefeld 33615, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 13-OCT-2014
            Assembly Method                   :: Newbler v. 2.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 60.6x
            Sequencing Technology             :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_001514215.1-RS_2024_04_21
            Annotation Date                   :: 04/21/2024 02:15:15
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,539
            CDSs (total)                      :: 7,454
            Genes (coding)                    :: 7,226
            CDSs (with protein)               :: 7,226
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 228
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 67 of 237
            Pseudo Genes (incomplete)         :: 196 of 237
            Pseudo Genes (internal stop)      :: 10 of 237
            Pseudo Genes (multiple problems)  :: 43 of 237
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 00:26:42
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 4970
            Orig. end                         :: 15468
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            KQ948859.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10498
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="melanin"
                     /region_number="1"
                     /rules="melC"
                     /tool="antismash"
     cand_cluster    1..10498
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="melC"
                     /kind="single"
                     /product="melanin"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10498
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="True"
                     /core_location="[5000:5498](-)"
                     /cutoff="20000"
                     /detection_rule="melC"
                     /neighbourhood="5000"
                     /product="melanin"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5498)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="melC"
                     /neighbourhood="5000"
                     /product="melanin"
                     /protocluster_number="1"
     misc_feature    10..24
                     /note="TFBS match to SypG, Regulator of biofilm formation
                     and host colonization, confidence: weak, score: 17.52"
                     /tool="antismash"
     gene            complement(13..405)
                     /locus_tag="AQJ66_RS19110"
                     /old_locus_tag="AQJ66_19115"
     CDS             complement(13..405)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016577257.1"
                     /locus_tag="AQJ66_RS19110"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AQJ66_19115"
                     /product="hypothetical protein"
                     /protein_id="WP_061923378.1"
                     /transl_table=11
                     /translation="MRKFQRAAVVAAVVAGLSTFGISASFAGGNGDAPQLTAVANSQAN
                     AVANWSAPAGHSMHDRGGKSDEGGKSEDGGKPEEGGKYDEGGKSDEGGSEYGGKSEDGG
                     KSEDGAKYADVVYGQFIAPFFSSFSK"
     misc_feature    446..460
                     /note="TFBS match to SypG, Regulator of biofilm formation
                     and host colonization, confidence: weak, score: 17.32"
                     /tool="antismash"
     misc_feature    complement(453..468)
                     /note="TFBS match to CRP, Cyclic AMP receptor protein,
                     confidence: medium, score: 16.52"
                     /tool="antismash"
     misc_feature    complement(467..482)
                     /note="TFBS match to FuR_variant_1, Ferric Uptake
                     Regulator, confidence: weak, score: 16.68"
                     /tool="antismash"
     misc_feature    470..485
                     /note="TFBS match to FuR_variant_1, Ferric Uptake
                     Regulator, confidence: weak, score: 16.74"
                     /tool="antismash"
     misc_feature    475..489
                     /note="TFBS match to SypG, Regulator of biofilm formation
                     and host colonization, confidence: weak, score: 19.21"
                     /tool="antismash"
     gene            complement(528..893)
                     /locus_tag="AQJ66_RS19115"
                     /old_locus_tag="AQJ66_19120"
     CDS             complement(528..893)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016436732.1"
                     /locus_tag="AQJ66_RS19115"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AQJ66_19120"
                     /product="hypothetical protein"
                     /protein_id="WP_061923381.1"
                     /transl_table=11
                     /translation="MVSRKKMAVVSGLLGGLVATCAGAAQAHAAAGPGTCTRDLLGNVT
                     CVQRITGEMPPGDGFTAPRVQTCLPTEPLTLPTSPMSNGTTQIGPKVTCAGPAPVLGTA
                     DEGDKGDGSSGLARLLS"
     misc_feature    886..901
                     /note="TFBS match to RutR, Regulator of the pyrimidine and
                     purine metabolism, confidence: weak, score: 19.41"
                     /tool="antismash"
     gene            complement(1057..1299)
                     /locus_tag="AQJ66_RS19120"
                     /old_locus_tag="AQJ66_19125"
     CDS             complement(1057..1299)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018537853.1"
                     /locus_tag="AQJ66_RS19120"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AQJ66_19125"
                     /product="hypothetical protein"
                     /protein_id="WP_061923385.1"
                     /transl_table=11
                     /translation="MRKLQQVVIAAVAAGGLSAVGVGAGTAFAHDNGPGMSDLYRPYQE
                     CSPQTVAENDVPVAALGLGQTFDTTCGQFNHAFTR"
     misc_feature    1290..1305
                     /note="TFBS match to CopR, Copper-responsive regulator,
                     confidence: weak, score: 17.33"
                     /tool="antismash"
     gene            complement(1450..2850)
                     /locus_tag="AQJ66_RS19125"
                     /old_locus_tag="AQJ66_19130"
     CDS             complement(1450..2850)
                     /GO_function="GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /NRPS_PKS="Domain: Condensation_LCL (65-328). E-value:
                     5.3e-20. Score: 63.6. Matches aSDomain:
                     nrpspksdomains_AQJ66_RS19125_Condensation_LCL.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Condensation"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1209: polyketide synthase associated protein papA3"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019980760.1"
                     /locus_tag="AQJ66_RS19125"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AQJ66_19130"
                     /product="condensation domain-containing protein"
                     /protein_id="WP_061923388.1"
                     /sec_met_domain="Condensation (E-value: 1e-16, bitscore:
                     54.8, seeds: 42, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRISDIQRCEVRPGRLVEWTFSPATVATAAALPTDSRPPAYNQES
                     HIRTARTVREDGLFVPTWLGAAFDLPGRADLDALEEALRAWTLRHETLRSGFRWSGDEM
                     HRFTLERDDVSLRREVVGDFTDTGRLVRHLQDRFDVAADALGWPNLIYAAVVRDDSTSV
                     YLAFDHTNVDAYSLQRIPDEINELYAARLAGHAVGGTQVGSYVDFCELERTNADGIDDT
                     HAIVGRWREFIGRCDGVLPNFPVDLGLDPGGRLPTQKLMREWLVDADAAAAFEAYCRRH
                     GGSLVGVLAATSMIVHEIGGQPVYRTVVPFHTRMKSAWSDSVGWYVGGAPIEVPAARDF
                     DEALAVVRSELQANRSLARMPLARVLRLLGADFRPTSPDLYSIVSYVDARVAPGATRWS
                     ERKAYGLLRVSYGDQVCVWVNRLDEGLWFACRYPDTDAAYKNMRRYVEALRGLILSVPA
                     SPLAAHGR"
     aSDomain        complement(1867..2655)
                     /aSDomain="Condensation"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_AQJ66_RS19125_Condensation_LCL.1
                     "
                     /domain_subtypes="Condensation_LCL"
                     /evalue="5.30E-20"
                     /label="AQJ66_RS19125_Condensation_LCL.1"
                     /locus_tag="AQJ66_RS19125"
                     /protein_end="328"
                     /protein_start="65"
                     /score="63.6"
                     /specificity="C activity: active"
                     /specificity="Active site motif: DHTNVDA"
                     /tool="antismash"
                     /translation="AAFDLPGRADLDALEEALRAWTLRHETLRSGFRWSGDEMHRFTLE
                     RDDVSLRREVVGDFTDTGRLVRHLQDRFDVAADALGWPNLIYAAVVRDDSTSVYLAFDH
                     TNVDAYSLQRIPDEINELYAARLAGHAVGGTQVGSYVDFCELERTNADGIDDTHAIVGR
                     WREFIGRCDGVLPNFPVDLGLDPGGRLPTQKLMREWLVDADAAAAFEAYCRRHGGSLVG
                     VLAATSMIVHEIGGQPVYRTVVPFHTRMKSAWSDSVGWYVG"
     CDS_motif       complement(2557..2658)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_AQJ66_RS19125_0001"
                     /evalue="8.60E-08"
                     /label="C2_LCL_024-062"
                     /locus_tag="AQJ66_RS19125"
                     /protein_end="98"
                     /protein_start="64"
                     /score="24.6"
                     /tool="antismash"
                     /translation="GAAFDLPGRADLDALEEALRAWTLRHETLRSGFR"
     gene            complement(3034..3993)
                     /locus_tag="AQJ66_RS19130"
                     /old_locus_tag="AQJ66_19135"
     CDS             complement(3034..3993)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) 2-Hacid_dh_C"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1072: dehydrogenase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010468355.1"
                     /locus_tag="AQJ66_RS19130"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AQJ66_19135"
                     /product="D-2-hydroxyacid dehydrogenase family protein"
                     /protein_id="WP_061923391.1"
                     /sec_met_domain="2-Hacid_dh_C (E-value: 8.2e-56, bitscore:
                     182.1, seeds: 135, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRLRCAVLDDFQNAALTSADWSAVQDRVEVVGFREHIADEDELAH
                     VLADFDIVVTLRERVPFPASLLDRLPRLKLLVASGMRNSVLDYAAAERGGVTVCGTASS
                     STPPVEMTWALLLGLARGLVTENGALRTGGPWQSTVGADLHGRRLGLLGLGNIGGRVAR
                     VGLAFGMRVTAWSQNLTQERAAEVGAERAASKEELLRTSDFVSLHLKLSERTRGLLGAA
                     ELALLKPTAYLINTSRAGLVDQDALLAALREGRIAGAGVDVFDTEPLPADHPMRTAPRL
                     LATPHLGYVSEANYATYYGQAVEDIEAFLAGSPIRRLG"
     gene            complement(4151..5017)
                     /locus_tag="AQJ66_RS19135"
                     /old_locus_tag="AQJ66_19140"
     CDS             complement(4151..5017)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019325459.1"
                     /locus_tag="AQJ66_RS19135"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AQJ66_19140"
                     /product="tyrosinase family protein"
                     /protein_id="WP_061923394.1"
                     /transl_table=11
                     /translation="MVYTRKNVSTLTPTERRRFVNALLTVKRRGEYDEFVRTHIEYYTP
                     DGEQGLRAAHMAPSFLPWHRKFLLELERALRRVDASVTVPYWDWTRDRTARSVPWTADL
                     LGGNGRASDRQVMTGPFAYGAGHWPVKVNVTDGHFLTRDLGRARSPIQLPTAAELEGAL
                     SDPVYDVAPWDSTVGRGFRNKLEGWGAGRGSVSWRNHNRVHRWVGGLMVGGASVNDPVF
                     WLHHAFVDLQWTRWRRRHPGARYLPAEPPGAGDAQHDRVVARHERMPPWDVTPHELEDL
                     SDVYRYE"
     gene            complement(5001..5498)
                     /locus_tag="AQJ66_RS19140"
                     /old_locus_tag="AQJ66_19145"
     CDS             complement(5001..5498)
                     /GO_function="GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /GO_process="GO:0042438 - melanin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     melanin: melC"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007499741.1"
                     /locus_tag="AQJ66_RS19140"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AQJ66_19145"
                     /product="tyrosinase family oxidase copper chaperone"
                     /protein_id="WP_061923397.1"
                     /sec_met_domain="melC (E-value: 5.2e-49, bitscore: 160.1,
                     seeds: 38, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MVTGVGGTPGCGQRGTPAAGGAESRGRTRRHAVRGMLLTAVTAAL
                     APVVAACRQPRAADGRARRAAAFDETYRGRRIRGAEVRAEGAAGSRWDVTVDGRPLHLM
                     RRADGTWLSMVDHYCSYPTPLAAARAAVDELGPGEHLRDLAPGPMDGGPVHAGGGEHGV
                     HA"
     gene            <5610..5929
                     /locus_tag="AQJ66_RS37385"
                     /pseudo=""
     CDS             <5610..5929
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016435064.1"
                     /locus_tag="AQJ66_RS37385"
                     /note="frameshifted; incomplete; partial in the middle of a
                     contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /product="long-chain fatty acid--CoA ligase"
                     /pseudo=""
                     /transl_table=11
                     /translation="VGGNRPFVAAPVTLGPEAVAPWLPVRELPPDRRRRPSSGSLGRAP
                     RCGGPWTTPARPSPGPSRSAPSRRSRARPPRGTGCSRRP"
     gene            6145..6780
                     /locus_tag="AQJ66_RS38880"
                     /old_locus_tag="AQJ66_19150"
     CDS             6145..6780
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="AQJ66_RS38880"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="AQJ66_19150"
                     /product="hypothetical protein"
                     /protein_id="WP_159052584.1"
                     /transl_table=11
                     /translation="MRQTLTKGLVVAAAATSALSLYGTWAYADSGSRTAADSSAAALSG
                     NTVHRNTPAPLDALKPVLGDGTSGYGDDKSAGDTSKDSAIGTPDTSGTGSSTAGSVSVT
                     SDTKSDDTPGYGASGYGSDDSSDGYGSDDCSCGSPTPPPPTVTPAPPAKPTPTPSKTPP
                     AKAVVPPAAPPVMAETGAEGTAAAVAGSAALLAAGTVLYRRGRTSARR"
     misc_feature    6810..6825
                     /note="TFBS match to AfsQ1, Two-component system AfsQ1-Q2,
                     activator of antibiotic production, confidence: weak,
                     score: 16.86"
                     /tool="antismash"
     gene            6931..8160
                     /locus_tag="AQJ66_RS19150"
                     /old_locus_tag="AQJ66_19155"
     CDS             6931..8160
                     /GO_function="GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006382383.1"
                     /locus_tag="AQJ66_RS19150"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AQJ66_19155"
                     /product="glycosyl hydrolase"
                     /protein_id="WP_107118974.1"
                     /transl_table=11
                     /translation="MTSARRWSTLRRRLVLITAGALASAAIAQAPALESIGAADRPDPL
                     PCAQTAPSACPADGTRAGSAAASPSPAFGAYLGYGPPGVERTRAFGSWLDGAEVRVGHT
                     YLPGDLWSNIEGAPGFLDSWAAWRRARADRLLVLNVPLMEHNEVPVPDARVRGLLRRAA
                     TGEFDEHFTSLARRLVGLGVPDTILVLGWEMNGVTYTHRCGPDPERWKAYWRRIVTAMR
                     SVPGQRFRFDFAPNRGADAVPWPSCYPGDDVVDIVGMDSYDQPEGVSFDEQVSEPYGLQ
                     AQVDFARAHGKPFSYPEWGLFRNGDNRSYMLRMLAWLDQHPPLYQTITDYCPHGVWQCS
                     DNPRASAVYRAALSGPPAPDPARPAVPTPPVPTAPPEPRPSPNPTPPAVVTPRPPHHCP
                     CRPPGRPTGG"
     gene            complement(8164..9291)
                     /locus_tag="AQJ66_RS19155"
                     /old_locus_tag="AQJ66_19160"
     CDS             complement(8164..9291)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007505671.1"
                     /locus_tag="AQJ66_RS19155"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AQJ66_19160"
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="WP_061923402.1"
                     /transl_table=11
                     /translation="MTAGTCRALSVEVCTDAPAFGRLAGEWERLYRACRAATPFQNHAW
                     LHSWWLSYGTPGALRVVLVRRSGELVAAAPLMRVHRPLPALVPVGGAISDFCDVLLDEA
                     AADLAVPALADALAGLARTALIDFREVRPGSAVERVYHCWQGPRHRVPDSLCLELPAVP
                     MDQLVGRLSSKRAQRVRNRMSKLARIGVRWRSVGPDETGAALGRLIELHRLQWEGRKVT
                     PEHLRPRFLDHLVRSVVPMVRCGEAVVREFLLEDEVVAVDLTLQSRQLAGKYLYGVHPR
                     LRERKVDVTTMLLIAAVEELPPQGTAEGPVLSLLRGTEPYKYQWRPRGIVNQRLLLARR
                     RTAPLLAAAACEAAARARAKQLLGRAPADPQPAAA"
     gene            complement(9288..9953)
                     /locus_tag="AQJ66_RS38345"
                     /old_locus_tag="AQJ66_19165"
     CDS             complement(9288..9953)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004001117.1"
                     /locus_tag="AQJ66_RS38345"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AQJ66_19165"
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="WP_061923405.1"
                     /transl_table=11
                     /translation="MTDDHPTEPVRPPRRSRWRAVLRWLLVPSGVLLGASAGAAYGVMT
                     PPQYTATSYVIAVPADKAAPDSAATLGFAQTYGRVATQLAVLGRAQDAAGVPVRTLRRS
                     VRAETSPDAPMIAISATSRRRGRATAIADAVSRALTTQAERAKRSTGVSLVLLSRAVPP
                     TEPSSPSTVLTALVGASAGGLLGGLGLLARPRRAPADDDRARAQVPVQAPAAEPQGVR"
     misc_feature    10348..10362
                     /note="TFBS match to VqsM, Regulator of quorum-sensing
                     signalling systems, confidence: medium, score: 17.92"
                     /tool="antismash"
ORIGIN
        1 gatgcgcctg cctcacttgg agaagctgga gaagaacggg gcgatgaact ggccgtagac
       61 gacgtccgcg tacttggcgc cgtcctccga cttgccgccg tcctccgact tgccgccgta
      121 ctcggaaccg ccctcgtccg acttgccgcc ctcgtcgtac ttgccgccct cctccggctt
      181 gccaccgtcc tcggacttgc cgccctcgtc ggacttgcca ccccggtcgt gcatggagtg
      241 cccggcggga gcgctccagt tggcaacggc gttggcctgc gagttggcca cggcggtgag
      301 ctgcggagcg tcaccgttgc cgccggcgaa gctggcgctg atgccgaagg tggacaggcc
      361 cgcgacgacc gcggcaacga ccgcagcacg ctggaacttg cgcatggttg accctttcgt
      421 acccgggcat gacgccctgg ttgactactt aatgtgagca tagacacaca ttcagtatca
      481 ttttgggatc tgtacttatg cgccgtgtcg gcaggtgcgg ccgcgactca gctcagcagg
      541 cgcgccagcc ccgaactccc gtcacccttg tcaccctcgt cggccgtacc gaggaccggc
      601 gccgggccgg cgcaggtcac cttggggccg atctgcgtgg tgccgttgct catgggggac
      661 gtgggcagcg tcagcggttc cgtcggcagg caggtctgca cccggggggc ggtgaacccg
      721 tcacccgggg gcatctcgcc ggtgatccgc tggacgcagg tgacgttgcc cagcaggtcg
      781 cgcgtgcagg tgcccgggcc cgccgcggcg tgcgcctggg cggcgccggc acaggtcgcg
      841 acgaggccgc cgagcagccc cgagacgacc gccatcttct tcctgctgac catgtggtgc
      901 gattccttcg tgagggtgcc cgggcgccgt acaccgcgag ccggccgcgc cgggcgtggg
      961 gggtgtcggg gcggaaaggc ccttgcggga cggccccttc gagggcactc ggaggaaacc
     1021 ccggagcgcg tcacgcgcgg ggccgcgccg ccgtcttcag cgggtgaagg cgtggttgaa
     1081 ctgaccgcag gtggtgtcga aggtctggcc gaggccgagc gcggcgaccg ggacgtcgtt
     1141 ctcggcgacc gtctgcgggc tgcactcctg gtacggacgg tagaggtcgg acattcccgg
     1201 tccgttgtcg tgggcgaagg cggtaccggc gccgacgccg acggcggaaa ggccaccggc
     1261 cgccactgct gcgatcacga cctgctgaag cttacgcatg gaaccctcgg ttcttcattt
     1321 gcctgtacag agagactgat tgcctaccgt gacgaactga acactagcag tagtcgacca
     1381 gggtgttcca catcggccga tacccgtgtc catacgggga gatgggcgca tcccggggcg
     1441 aagaggacgt caacgcccgt gcgcggccag gggacttgcg ggcaccgaca ggatcaggcc
     1501 ccgcagtgcc tcgacgtacc gccgcatgtt cttgtacgcc gcgtccgtgt ccgggtagcg
     1561 gcaggcgaac cacagtccct cgtcgaggcg gttgacccag acgcacacct ggtcgccgta
     1621 ggagacccgg agcagcccgt acgccttccg ctcggaccag cgcgtggcac cgggggcgac
     1681 acgggcgtcg acgtacgaga cgatcgagta gaggtcgggc gaggtcgggc ggaagtctgc
     1741 gccgagcagg cgcaggaccc gggccagcgg catgcgggcc agcgagcggt tggcctggag
     1801 ttcggagcgc accacggcga gcgcttcgtc gaagtcccgg gccgcgggca cctcgatcgg
     1861 cgctccgccg acgtaccagc cgacggagtc cgaccaggcg gacttcatcc gggtgtggaa
     1921 cggcacgacc gtgcggtaga ccggctgtcc gccgatctcg tggacgatca tgctggtcgc
     1981 ggccaggacg cccaccaggc tgccgccgtg gcgccggcag tacgcctcga acgcggcggc
     2041 ggcgtcggcg tccacgagcc actcgcgcat gagtttctgg gtggggagcc ggccgccggg
     2101 gtcgaggccg agatcgacgg ggaagttggg cagcacgccg tcgcagcggc cgatgaactc
     2161 ccgccagcgg ccgacgatcg cgtgggtgtc gtcgatgccg tccgcgttgg tccgttccag
     2221 ctcgcagaag tcgacgtagc tgccgacctg cgtgccgccc acggcgtggc cggcgagccg
     2281 cgccgcgtac aactcgttga tctcgtccgg gatgcgctgc agcgagtagg cgtcgacgtt
     2341 ggtgtggtcg aaggccaggt acacgctggt ggagtcgtcg cggacgacgg ccgcgtagat
     2401 gaggttcggc cagcccagcg cgtcggcagc gacgtcgaaa cggtcctgca ggtgccgtac
     2461 gagcctgccg gtgtcggtga agtcgccgac cacctcgcgg cgcagcgaca cgtcgtcccg
     2521 ctcgagcgtg aaccggtgca tctcgtcgcc ggaccagcgg aagccgctgc gcagcgtctc
     2581 gtgccgcagc gtccaggcgc gcagcgcctc ctccagcgcg tcgaggtcgg cgcggccggg
     2641 caggtcgaag gccgcgccca gccaggtcgg cacgaacagg ccgtcctcgc gcaccgtgcg
     2701 cgccgttctg atatgcgact cctggttgta cgccggcggc cgtgagtccg tcggcagcgc
     2761 cgccgccgtc gcgacggtcg ccggactgaa cgtccactcg acgagccgtc cgggccggac
     2821 ctcgcagcgc tggatgtcag aaattcgcac gggcgtctcc gttcgcagat ggcacagaat
     2881 cgatcaaggg gatgccgtgc gcgcgcgtgg tccccgccgt gtcgccgaca ttcagcggaa
     2941 cgtgtggcgt ctcgaacggc gaagcgaagc acccgccgaa ccgggctccg ggaagggccc
     3001 gttcggatgt ccggcgggtg gccgcggcga cggtcagccg aggcggcgta tcggggagcc
     3061 ggccaggaac gcctcgatgt cctccacggc ctggccgtag tacgtcgcgt agttggcctc
     3121 ggacacgtag ccgaggtggg gggtggcgag caggcgcggg gcggtgcgca tcgggtggtc
     3181 cgccgggagc ggttcggtgt cgaagacgtc gacgccggcg ccggcgatcc ggccctcgcg
     3241 caaagcggcg agcagggcgt cctggtcgac gaggccggcg cgcgaggtgt tgatcaggta
     3301 cgcggtcggc ttgagcaggg cgagttcggc cgcgccgagc aggccccggg tgcgctcgct
     3361 cagtttgagg tggagggaga cgaagtcgct ggtgcgcagc agttcctcct tggaggcggc
     3421 ccgttcggcg cccacctcgg cggcgcgctc ctgggtgagg ttctggctcc aggcggtgac
     3481 ccgcatgccg aaggcgagac cgacccgggc cacccggccg ccgatgttgc cgaggccgag
     3541 cagtccgagg cggcggccgt gcaggtcggc gccgaccgtg gactgccacg ggccaccggt
     3601 gcgcagcgcg ccgttctcgg tcaccaggcc gcgggcgagg cccagcagca gcgcccaggt
     3661 catttctacg ggcggggtcg aggagctggc cgttccgcac accgtcacac cgccccgctc
     3721 ggcggccgcg tagtcgagca ccgagttgcg catgccggag gcgacgagca gcttcaggcg
     3781 gggcaggcgg tcgagcaggg aggccgggaa ggggacccgt tcgcgcaggg tgacgacgat
     3841 gtcgaagtcg gccaggacgt gcgcgagttc gtcctcgtcg gcgatgtgct cgcgaaagcc
     3901 gacgacctcc acccggtcct ggacggcgga ccagtcggcg gaggtgagcg cggcgttctg
     3961 gaagtcgtcg agcacggcac agcgcagtcg catgtcggtg tgtctcccca gtcggtgtgg
     4021 tgcgtggcgg cgccggcggc cggggccgcc ggcggtccca gtccaccact ggcgcacccg
     4081 ggccggtgaa cgggccccgg ctcccgggtg agccggggcc gggcgcccgc ccgtccgccg
     4141 cgaaccggcc tcactcgtac cggtagacgt cgctcaggtc ctccagttcg tggggcgtca
     4201 cgtcccacgg cggcatccgc tcgtgccggg ccaccacccg gtcgtgctgg gcgtcgccgg
     4261 cgccgggcgg ctcggccggg aggtaccggg cgccggggtg gcgccgccgc cagcgggtcc
     4321 actgcaggtc cacgaaggcg tggtgcagcc agaagacggg gtcgttgacg gaggcgccgc
     4381 cgaccatcag cccgccgacc cagcggtgga cccggttgtg gttgcgccag gacacgctgc
     4441 cccggcccgc gccccagccc tccagcttgt tgcggaaccc ccggccgacc gtggagtccc
     4501 agggcgccac gtcgtagacg gggtcggaca gcgcgccctc cagctcggcg gcggtgggca
     4561 gttggatcgg ggagcgggcg cggccgaggt cgcgggtgag gaagtggccg tccgtgacgt
     4621 tcactttgac gggccagtgg cccgcgccgt aggcgaacgg cccggtcatc acctgccggt
     4681 ccgaggcgcg tccgttgccg ccgagcaggt cggcggtcca cggcacggaa cgggccgtgc
     4741 ggtcgcgggt ccagtcccag tacggcaccg tcaccgaggc gtcgacccgg cgcagcgccc
     4801 gctccagttc cagcaggaac ttgcggtgcc agggcaggaa ggagggtgcc atgtgggcgg
     4861 cgcgcaggcc ctgttcgccg tcgggcgtgt agtactcgat gtgcgtgcgc acgaactcgt
     4921 cgtactcgcc gcgccgtttg accgtcagca gggcgttgac gaaccgccgc cgctcggtgg
     4981 gggtcagggt gctgacgttc ttacgcgtgt acaccatgct ccccacctcc cgcgtgcacc
     5041 ggtcccccgt ccatcgggcc gggcgccagg tcgcgcaggt gctcgccggg gccgagttcg
     5101 tcgaccgccg cgcgggccgc ggcgagcggc gtcgggtacg agcagtagtg gtcgaccatg
     5161 ctcagccagg tgccgtcggc gcggcgcatc aggtgcagcg ggcggccgtc gacggtgacg
     5221 tcccagcggc tgccggcggc cccctcggcg cggacctcgg cgccccggat gcggcggccg
     5281 cggtaggtct cgtcgaaggc cgcggcgcgc cgggcgcggc cgtccgcggc gcgcggctgc
     5341 cggcaggccg cgacgaccgg ggcgagggcg gcggtcacgg ccgtgaggag cattccccgt
     5401 acggcgtgcc gccgtgtcct cccccgggac tccgccccgc ccgcggccgg agttccgcgc
     5461 tgcccgcacc ccggcgttcc gccgacgcct gtgaccatcg ttcactcctc gtccggttcg
     5521 gttgtccctc ccggtaaccg ccgcacgggc cttccggtca ccgcggtgcg gccggaccgg
     5581 tgacccgagc cggccggcgg gcgaggagcg tcggcggcaa ccggcccttc gtcgccgcgc
     5641 cggtcaccct cggccccgag gcggtcgccc cctggctgcc ggtccgcgaa ctgcccccgg
     5701 accgccgacg gcgtccctcg tccgggtccc tcggccgcgc gccgaggtgc ggcgggccgt
     5761 ggaccacgcc ggcgcggccg tctcccgggc cgagtcgatc cgcgccttcg cgccggtcga
     5821 gggcgcgtcc accgagggga acgggctgct cacggcgtcc ctgaagggga agcggcacgc
     5881 ggtgccggcc gcgtacgcgg acgaggtgga ggccctgtac ggcgggtgac cgagccaggg
     5941 cgggcaatcg ggtgaattcc gcgccattca cggcgtctcg gtttcttcgg gcctccatcg
     6001 gtcgttcaca gtccgcaggt cgtggcgagt gcgaacgtgc ggcatcacgg tccctgcgcc
     6061 gacccctgcg gaatccggca cacgaggcga tcgcatcgac gaccggcgcc ggcttccctt
     6121 cccgccgaga gaaagacaac gagagtgcga cagactctga ccaagggatt ggtcgtggcc
     6181 gccgccgcga cgagcgccct gtctctgtac ggcacctggg cctacgcgga ctccggctcg
     6241 cgtacggcgg cggactcgtc cgccgccgcg ctgtcgggca acacggtgca caggaacacg
     6301 ccggctccgc tggacgccct gaagcccgtg ctcggagacg gcacgtccgg atacggcgac
     6361 gacaagtccg ccggcgacac gtcgaaggac tccgccatcg ggacgccgga cacctccgga
     6421 accggctcct ccacggccgg ttccgtgagc gtcacctccg acaccaagtc ggacgacacg
     6481 ccgggctacg gggcgtcggg ctacggctcc gacgactcct ccgacggtta cggctccgac
     6541 gactgctcgt gcggctcgcc cacgccgccg cccccgaccg tcacgccggc acccccggcg
     6601 aagcccactc cgaccccgtc gaagacgccg cccgccaagg ccgtcgtgcc gccggccgcg
     6661 cctcccgtca tggccgagac cggcgccgag ggcacggccg cggccgtggc cggcagcgcc
     6721 gcgctgctgg ccgccggcac cgtcctgtac cggcggggcc ggacctcggc ccgccggtag
     6781 gtcacggagg tgccggacgc ctcgcgtccg gcacctcgcg gtcacccgtg cgggctaccg
     6841 gccgccgagc cgcccgactc gtttggtgca ttcgccgtat atatgactgt gcctcagctt
     6901 ctggggcgga gccgcaccga aggagtggga atgacgtctg cgcgtcggtg gtccaccctc
     6961 aggcggcgcc tggtgctcat caccgccgga gcactcgcgt cggcggcgat cgcccaggcc
     7021 cccgccctcg agtcgatcgg cgccgcggac cggcccgacc cgctgccgtg cgcgcagacg
     7081 gccccgtccg cgtgcccggc cgacgggacg cgggccggct ccgctgcggc gtccccgtcg
     7141 cccgccttcg gcgcgtacct cggctacggg ccccccggcg tggagcgcac gagggccttc
     7201 ggctcctggc tcgacggcgc cgaggtgcgc gtggggcaca cctacctgcc gggcgacctg
     7261 tggagcaaca tcgagggcgc gccgggcttc ctcgacagct gggccgcctg gcggcgggcc
     7321 cgggccgacc ggctgctcgt gctcaacgtg cccctgatgg agcacaacga ggtgccggtg
     7381 cccgacgcgc gggtcagggg gctgctgcgc cgcgcggcga ccggcgagtt cgacgagcac
     7441 ttcacttccc tggcccggcg cctggtcggc ctgggggtgc cggacaccat cctggtgctc
     7501 ggctgggaga tgaacggcgt cacctacacc caccgctgcg gccccgaccc ggagcgctgg
     7561 aaggcgtact ggcggcgcat cgtcaccgcc atgcgctcgg tccccgggca gcggttccgg
     7621 ttcgacttcg ccccgaaccg gggcgcggac gcggtgccgt ggccgagctg ctaccccggg
     7681 gacgacgtcg tcgacatcgt cggcatggac tcctacgacc agcccgaggg cgtctccttc
     7741 gacgagcagg tgtccgagcc gtacgggctg caggcccagg tcgacttcgc ccgggcccac
     7801 ggcaagccgt tctcgtatcc cgagtggggc ctgttccgca acggcgacaa caggtcgtac
     7861 atgctgcgca tgctcgcgtg gctggaccag cacccgccgc tgtaccagac gatcaccgac
     7921 tactgcccgc acggcgtgtg gcagtgctcg gacaatcccc gggcctccgc cgtctaccgc
     7981 gcggcgctgt ccggcccgcc cgcaccggac ccggcacggc ccgcggtccc caccccgccg
     8041 gtgcccacgg ctcccccgga gccgcgcccg agcccgaacc cgacaccgcc ggccgtggtc
     8101 acgccccgcc caccgcacca ctgcccctgc cggccgccgg ggcggcccac cgggggctag
     8161 ggtctaggcc gccgcgggct gcgggtcggc cggtgcccgg cccaggagct gcttggcccg
     8221 ggcgcgcgcg gcggcctcgc aggcggcggc cgcgagcagc ggcgcggtgc gccgacgggc
     8281 cagcagcaga cgctggttga cgatgccgcg gggccgccac tggtacttgt acggctcggt
     8341 accgcgcagc aggctgagca ccgggccttc cgcggtcccc tgcgggggca gttcctcgac
     8401 ggccgcgatg agcagcatcg tcgtcacgtc caccttgcgc tcgcgcagcc gcggatggac
     8461 gccgtacagg tacttcccgg ccagctgccg ggactgcaag gtcaggtcga cggccacgac
     8521 ctcgtcctcc agcaggaact ccctcaccac ggcctcgccg caccggacca tcggcaccac
     8581 cgagcggacc aggtggtcga ggaaccgcgg ccgcaggtgc tccggggtca ccttccggcc
     8641 ctcccactgc aaccggtgca gttcgatgag ccggccgagc gccgcgccgg tctcgtcggg
     8701 gcccacggag cgccaccgga cgccgatccg ggcgagcttg ctcatccggt tgcggacccg
     8761 ctgggcccgc ttcgacgaca gccgcccgac cagttggtcc atcgggacgg ccggcagctc
     8821 caggcacagg gagtcgggca cccggtgccg cgggccctgc cagcagtggt acacccgctc
     8881 caccgcgctg ccgggccgca cctcacggaa gtcgatcagc gcggtacggg ccaggccggc
     8941 cagggcgtcc gccagcgccg ggaccgccag gtcggccgcc gcctcgtcga ggaggacgtc
     9001 gcagaagtcg gagatcgccc cgccgacggg caccagcgcc ggcagcgggc ggtgcacccg
     9061 catgagcggc gcggcggcca ccagctcacc gctccggcgc accaggacca cccgcagcgc
     9121 gcccggcgtg ccgtacgaca gccaccacga gtgcagccag gcgtggttct ggaacggcgt
     9181 cgccgcccgg cacgcccggt acagccgctc ccactccccg gccagccgcc cgaacgcggg
     9241 ggcgtcggtg cacacctcca ccgacagggc gcggcaggtc ccggcggtca ccgcaccccc
     9301 tgcggttccg cggcgggggc ctggacgggc acctgcgccc gcgcccggtc gtcgtccgcc
     9361 ggggcccgcc gcggccgggc cagcaggccg aggccgccca ggaggccgcc ggcgctcgcg
     9421 ccgacgagtg cggtcagcac cgtggagggg gacgacggct cggtgggcgg gacggcccgg
     9481 gagagcagga cgagcgagac accggtgctc cgcttggccc gctcggcctg tgtggtcagg
     9541 gcgcgcgaga cggcgtcggc gatggcggtg gcccggccgc ggcgccggga cgtcgccgag
     9601 atcgcgatca tcggcgcgtc gggcgaggtc tcggcccgta cgctccggcg cagcgtccgc
     9661 accggtacgc ccgccgcgtc ctgggccctg cccagcacgg cgagctgcgt cgcgacccgg
     9721 ccgtacgtct gggcgaaccc gagggtcgcc gcggagtcgg gtgccgcctt gtcggccggt
     9781 acggcgatga cgtagctcgt ggcggtgtac tgcggtggcg tcatcacgcc gtacgcggcg
     9841 ccggcggacg cgccgagcag gacgccggac gggaccagca gccatcgcag caccgcccgc
     9901 cagcggcttc ggcggggcgg tcgtacgggt tccgtggggt ggtcgtcggt catcagggcc
     9961 tcacttccag ggtcgagccg gccaggacgg ccgtgtacac gtccatgagc tggcgggcgg
    10021 tgcggtcgac ggcgtagtgc cgcaccgccg gggggatgct gcggtcgccg ggtcccgccc
    10081 cgcgcagccg cagcagctcc cgggcgtacg cgcctgcgcc gcccgggacg cgccgggcgc
    10141 ccggtgcgct gcccggcggc aggtcgtcga cggcgggaca ggtcgtgtag agcacgggga
    10201 ggccggccgc gagcgcctcc agcacggcca ggccgaaggc ctcctcggcc gacggggcgg
    10261 cgagcacgtc catcgccgac agcaggccgg gcaggccggg cgcggggtcc gccgggtccg
    10321 gcaggtacgg ccgttcgccg gtcagcacga cccggtcggc cacccccagg tcgcgggcgg
    10381 tgcgccgcag cgcccgctcc tcgtccccgc cgccgaccag cagcagccgt acgtcccgcg
    10441 ggagttctcc cagcgcgcgg acgaggacgc cgaagcgttt gccggcggtg aggcgccc
//
