LOCUS       NZ_KQ948851            11308 bp    DNA     linear   CON 21-APR-2024
DEFINITION  Streptomyces bungoensis strain DSM 41781 PRJNA299233_s001, whole
            genome shotgun sequence.
ACCESSION   NZ_KQ948851
VERSION     NZ_KQ948851.1
KEYWORDS    WGS; RefSeq.
SOURCE      Streptomyces bungoensis
  ORGANISM  Streptomyces bungoensis
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 1011152)
  AUTHORS   Ruckert,C., Winkler,A., Kalinowski,J., Kampfer,P. and Glaeser,S.
  TITLE     Draft genome sequence of Streptomyces bungoensis DSM 41781, type
            strain for the species Streptomyces bungoensis
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1011152)
  AUTHORS   Ruckert,C., Winkler,A., Kalinowski,J., Kampfer,P. and Glaeser,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2015) CeBiTec, Bielefeld University, Sequenz 1,
            Bielefeld 33615, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 13-OCT-2014
            Assembly Method                   :: Newbler v. 2.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 60.6x
            Sequencing Technology             :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_001514215.1-RS_2024_04_21
            Annotation Date                   :: 04/21/2024 02:15:15
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,539
            CDSs (total)                      :: 7,454
            Genes (coding)                    :: 7,226
            CDSs (with protein)               :: 7,226
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 228
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 67 of 237
            Pseudo Genes (incomplete)         :: 196 of 237
            Pseudo Genes (internal stop)      :: 10 of 237
            Pseudo Genes (multiple problems)  :: 43 of 237
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 00:26:42
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 254594
            Orig. end                         :: 265902
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            KQ948851.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..11308
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="3"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..11308
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..11308
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:6308](-)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..6308)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            705..1436
                     /gene="hemQ"
                     /locus_tag="AQJ66_RS00960"
                     /old_locus_tag="AQJ66_00960"
     CDS             705..1436
                     /EC_number="1.3.98.5"
                     /codon_start=1
                     /gene="hemQ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018564417.1"
                     /locus_tag="AQJ66_RS00960"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AQJ66_00960"
                     /product="hydrogen peroxide-dependent heme synthase"
                     /protein_id="WP_061914906.1"
                     /transl_table=11
                     /translation="MSDDAPTTESGRIPNKGKLAKDLNEVIRYTLWSVFKLKDVLPEDR
                     AGYADEVQELFDQLAAKDVTIRGTYDLSGLRADADLMIWWHAETSDQLQEAYNLFRRTK
                     LGRALEPVWSNMALHRPAEFNRSHIPAFLADETPRNYVSVYPFVRSYEWYLLADEDRRR
                     MLADHGKMARGFPDVRANTVASFSLGDYEWLLAFEADELHRIVDLMRHLRGSEARMHVR
                     EEVPFFTGRRKSVADLVAGLA"
     gene            complement(1447..3102)
                     /locus_tag="AQJ66_RS00965"
                     /old_locus_tag="AQJ66_00965"
     CDS             complement(1447..3102)
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /NRPS_PKS="Domain: Abhydrolase_1 (115-488). E-value:
                     2.7e-17. Score: 55.1. Matches aSDomain:
                     nrpspksdomains_AQJ66_RS00965_Abhydrolase_1.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF00561"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016432060.1"
                     /locus_tag="AQJ66_RS00965"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AQJ66_00965"
                     /product="alpha/beta hydrolase"
                     /protein_id="WP_061914908.1"
                     /sec_met_domain="PF00561 (E-value: 4.9e-21, bitscore: 71.2,
                     seeds: 48, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRAAALYSAVGSLLLTTLAAAPAGGAPGGPAAPAAPGAAELRGTA
                     VAAARARAAGVRFGACAAIPQAPAGLRCGTVEVPLDYAHPDGTRIRLTVSRMRATQQDP
                     QNSKRKVPRQGALVYNPGGPGGSGMRFPLVAKLPAWKRIAAAYDLVGYAPRGVGGSAPL
                     SCMDPAQFVEAPTESPEHPSEAYKQQRIARAKAYARGCAERSGSRLRFYTSLDNARDLD
                     VLRAALGEDRLTFMGASYGTYFGAVYATLFPSHVRRMVLDSVVDPDPQKVWYRNNLEQS
                     AAFEDRWADFRDWIARHDDVYRLGGTPQAVQRGYDTARGRLAAKPAGGKVGPGELQSAF
                     LTAGYDDVFWPGRAAALSAYLRGDEKPLVRLAAPNAQTAAQAENGNAVYTAVECNDAPW
                     PTDWTVWDRDNTSLARVAPFETWDNAWMNLPCAYWPAPRQRPLDVRTGPGELPPVLILA
                     AERDAATPYAGALEMRRRLSGSVLVTERDAGSHGIGGGPNRCVNAHLDAYLLEGRLPEQ
                     HAACAPHPEPQPQPPATGSADRQVLRDALKGKGA"
     aSDomain        complement(1639..2757)
                     /aSDomain="Abhydrolase_1"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_AQJ66_RS00965_Abhydrolase_1.1"
                     /evalue="2.70E-17"
                     /label="AQJ66_RS00965_Abhydrolase_1.1"
                     /locus_tag="AQJ66_RS00965"
                     /protein_end="488"
                     /protein_start="115"
                     /score="55.1"
                     /tool="antismash"
                     /translation="ALVYNPGGPGGSGMRFPLVAKLPAWKRIAAAYDLVGYAPRGVGGS
                     APLSCMDPAQFVEAPTESPEHPSEAYKQQRIARAKAYARGCAERSGSRLRFYTSLDNAR
                     DLDVLRAALGEDRLTFMGASYGTYFGAVYATLFPSHVRRMVLDSVVDPDPQKVWYRNNL
                     EQSAAFEDRWADFRDWIARHDDVYRLGGTPQAVQRGYDTARGRLAAKPAGGKVGPGELQ
                     SAFLTAGYDDVFWPGRAAALSAYLRGDEKPLVRLAAPNAQTAAQAENGNAVYTAVECND
                     APWPTDWTVWDRDNTSLARVAPFETWDNAWMNLPCAYWPAPRQRPLDVRTGPGELPPVL
                     ILAAERDAATPYAGALEMRRRLSGSVLVTERDA"
     gene            complement(3247..4050)
                     /locus_tag="AQJ66_RS00970"
                     /old_locus_tag="AQJ66_00970"
     CDS             complement(3247..4050)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014675656.1"
                     /locus_tag="AQJ66_RS00970"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AQJ66_00970"
                     /product="TIGR04222 domain-containing membrane protein"
                     /protein_id="WP_061914911.1"
                     /transl_table=11
                     /translation="MFWVLLLLLAWAFAGTACTRLCLAAVRAAAADSARAGADAGAATH
                     RERDLTLYEAAFLSGGPRRVADVTMVSMARQRRLLLAHTGWATVVDPRGRDEMERSVIG
                     AIGPEGQSRIAPVRAAAAGAEAVRRLADGLVRAGLAVPDGARTTVADGVRGVRAAALAV
                     CALGLTALLLPAPTGLPRLLVALWFALPLALTLSCLVIARAEVHPYSRWASPAGQRLLG
                     ALHRGPAGDGDERTFLTSVAVRGVRAIGEPELRAAFTHRDRPGRE"
     misc_feature    4053..4062
                     /note="TFBS match to ToxT, Regulator of MSHA biosynthesis,
                     confidence: weak, score: 13.4"
                     /tool="antismash"
     gene            complement(4148..4918)
                     /locus_tag="AQJ66_RS00975"
                     /old_locus_tag="AQJ66_00975"
     CDS             complement(4148..4918)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015657154.1"
                     /locus_tag="AQJ66_RS00975"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AQJ66_00975"
                     /product="DUF4142 domain-containing protein"
                     /protein_id="WP_061914914.1"
                     /transl_table=11
                     /translation="MRPRPPVKGRGMFNGTGLIIAGLAATAAALLFPILSYADRPADAA
                     SASLLSAQTVTTPYGPLSAQDRDFVTKVRLAGLWELPAGEMAERKGTTQAVRDAGRHLV
                     DGHTSLDSHDRTVATQLGVPLPNEPNAQQKQWLATLRAARGQEFDRRFAGILRLAHGRV
                     FSLVAQVRAGTQNALVRDLADDANTTVLDHIKVLEATGYVDFSALARDLAASPSAVPDP
                     PSVDPSAAVPVEPSPSATYPLPPAASTPPPSAAR"
     gene            complement(5001..6308)
                     /locus_tag="AQJ66_RS00980"
                     /old_locus_tag="AQJ66_00980"
     CDS             complement(5001..6308)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: DUF692"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016327585.1"
                     /locus_tag="AQJ66_RS00980"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AQJ66_00980"
                     /product="DUF692 domain-containing protein"
                     /protein_id="WP_061914916.1"
                     /sec_met_domain="DUF692 (E-value: 1.8e-94, bitscore: 311.6,
                     seeds: 7, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRRLGTGIGWRPEIADAVEGMPGIDWVEVVAENVCPGHLPESLLR
                     LRERGVTVIPHGVSLGLGGAERPDEGRLAALAERVEALGAPLVTEHVAFVRAGGALTAS
                     PRLEAGHLLPVPRTRDALEVLCENVRIAQRALPVPLALENIAALFSWPGEELTEAQFLS
                     ELVERTGVRLLVDVANLHTNHVNRGEDPAEALAGLPLEALAYVHVAGGFERDGVWHDSH
                     AHPVPRPVLDVLTGLASRVAPPGVLLERDENFPGPAELERELDEIRAAVAAGRPPGDGG
                     TATGTAAGTAGPADSAAPARPGARQRVAVAQAAALSCLVAGTPVPEGFDRVRMGVQARA
                     LAAKRASVVAKVAPELPVILGEGYREAFLAYARTRPMTDGYRRDALDFAGHLLKLGRPA
                     GARARRQLRTWWLDRSGPAPRSPGLRARARVLIGRA"
     gene            6456..7274
                     /locus_tag="AQJ66_RS36780"
     CDS             6456..7274
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) APH"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /locus_tag="AQJ66_RS36780"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="phosphotransferase"
                     /protein_id="WP_079060006.1"
                     /sec_met_domain="APH (E-value: 2.6e-18, bitscore: 62.6,
                     seeds: 156, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MGSGSWGRHAVESTGGRVVKRFPHGDGGRAAREWRALTLLARHAP
                     GLAPEPLQAGLAASEPVVVMSRLDGKPLRGAALSGEQIDALAEAVAAVHAALPSHVLDG
                     VPLRPGHQAELVGHLRAWAGTARPAAEGPVRQAMDAGLAWLAASGLEADGAWGVPPVFG
                     PGDGNLANYLWDGRRVRIVDFEESGRSDRAFELAEITEHVGSWVDGPLDVPAFLDRFDL
                     SPVEAARLRECRRLVALVWLFLLAADDPDHPGNPPGTAERQAGRVLDLLA"
     gene            7528..8865
                     /locus_tag="AQJ66_RS00990"
                     /old_locus_tag="AQJ66_00990"
     CDS             7528..8865
                     /EC_number="2.7.11.1"
                     /GO_function="GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA]"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_process="GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Pkinase"
                     /gene_functions="regulatory (smcogs) SMCOG1030:
                     serine/threonine protein kinase"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007379381.1"
                     /locus_tag="AQJ66_RS00990"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AQJ66_00990"
                     /product="serine/threonine-protein kinase"
                     /protein_id="WP_279617305.1"
                     /sec_met_domain="Pkinase (E-value: 1.4e-48, bitscore:
                     161.2, seeds: 54, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MGARIGSGGMADVYDGVDTRLRRPVAVKVFRPGPDAHCEDRLAAE
                     AVLLARLQHPGLVTVYDVGRYEDRTYLVMQLVDGPTLRSLLAGGTLPERRVAELGAALA
                     GALAHVHRAGIVHRDVKPSNVLVDPAGAPHLADFGIARVANTTVHTAPDVLIGTAAYLA
                     PEQVEGGRVGPAADVYALGLVLLECLKGELEYQGTAVEVAVARLHRPPTVPAWLSPDLA
                     ALLGAMTARDPEARPDAGQCARELARLCSADGAHALQQLPAPAEARVPRARAARHEADD
                     PVRTQEAPAARTGVPGNARPSRRLAVGTALAALSVALGTTLAVAPRTSGAGDDRAAAPS
                     SPAATGSRPGTVSPVRPLRAQPSSGTVDVSDPAAGSSGSHAEPAVQRVNSPGTPDSTYR
                     APAGRHGAEHRKPSPHRPAGTDGRPRHAAPHGGPHHGAPQKGKGHG"
     gene            complement(8880..9620)
                     /locus_tag="AQJ66_RS00995"
                     /old_locus_tag="AQJ66_00995"
     CDS             complement(8880..9620)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007503630.1"
                     /locus_tag="AQJ66_RS00995"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AQJ66_00995"
                     /product="peptidyl-tRNA hydrolase"
                     /protein_id="WP_061914919.1"
                     /transl_table=11
                     /translation="MTHVPATPADSPFRSEHAPRDAAPQFVLPLVVRIERSAPPARTDA
                     LETAARAVLTLLGDERSAGDGEWAEAVRGWEDARIRKVVRRARGAEWRRAEALPGITVT
                     GRSAEVRVFPPVPLDGWPKDLARLQVSGTELDDPEPPAGADPAAPVLWLNPGLTMTAGK
                     SMAQAGHGAQLAWWALSEEARTAWRKAGFPLAVRTAAPARWAELTGAGLPLVRDAGFTE
                     IAPGTTVAVEGGGQFASLPRRGRP"
     gene            complement(9676..9867)
                     /locus_tag="AQJ66_RS01000"
                     /old_locus_tag="AQJ66_01000"
     CDS             complement(9676..9867)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020125244.1"
                     /locus_tag="AQJ66_RS01000"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AQJ66_01000"
                     /product="hypothetical protein"
                     /protein_id="WP_061914922.1"
                     /transl_table=11
                     /translation="MTEKDDEDYCMIVDAARPPKADGPPYAECVLCREPTEYPESYKGV
                     TLCPVCEWQEAQRTACSG"
     gene            complement(9887..10735)
                     /locus_tag="AQJ66_RS01005"
                     /old_locus_tag="AQJ66_01005"
     CDS             complement(9887..10735)
                     /GO_function="GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /GO_process="GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1235: polysaccharide deacetylase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010983654.1"
                     /locus_tag="AQJ66_RS01005"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AQJ66_01005"
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="WP_061914925.1"
                     /transl_table=11
                     /translation="MIPPVRRIAAACALAATLAACGTSGAPEPAHHAPAKPTASPSPTR
                     PPVLAPGPGGLTPVFEHGPRTLGKTVALTFDADMTADEGPRAAAGEHFDNPDLIGALRA
                     LKVPATIFMTGRWAEQYPAQARSLGHDPQFEVANHSYSHYAFTADCYGLPTVGADHMRQ
                     DVERAYAALRQAGVPRPLPYFRFPGGCYDRQALRALQGVGVTAVQWDVISGDAFATDAD
                     AVVEQVLNGVKPGSVVVMHCTRSAAPTTERVVRAVVPELRKRGYRFAKVSELIAETNGR
                     R"
     misc_feature    10752..10765
                     /note="TFBS match to PsrA, Oleic acid responsive regulator,
                     confidence: weak, score: 17.53"
                     /tool="antismash"
     misc_feature    complement(10906..10918)
                     /note="TFBS match to MatP, Cell cycle regulator,
                     confidence: weak, score: 21.19"
                     /tool="antismash"
ORIGIN
        1 ggcgcccgca ccggcgacac ggcctggcgc gtcgtcgccg gcgagcgggt gctgcacgcc
       61 gacgcggtgg tcgtcgccgt ccccgcaccc gtcgccgccc ggctcctcac cgccgaggca
      121 ccggccgccg cggccgagct cgccgccgtc gagtacgcct ccatggccct ggtcaccctc
      181 gcctaccggc gcgccggcct cgccctcccc gagggcagcg gcttcctcgt cccgcccgtc
      241 gacggccaca ccatcaaggc gtccaccttc gcctcccgca agtggggctg gatcgccgag
      301 gaggacccgg acgtggtcgt gctgcgcacc tccgtcggcc gccacggcga gacggagatc
      361 ctcggccgcg aggacgccga cctggtggag gtctcccggc atgacctgcg cgaggccacc
      421 gggctggacg ccgtacccct ggcgagccgg gtcacccgct ggaccgacgg actgccgcag
      481 tacccggtgg gccaccacgc gcgcgtgcgg cgcatccgcg agcacctggc caagctgccc
      541 ggcctcgcgg tgtgcggcgc gccctacgac ggcgtcggca tcccggcctg cgtcgcgagc
      601 gcccacgcgg ccgtcgacca gctgggcggc gacctgcgcg gtgtggcgga actgacggcc
      661 cacccggtgc agagcctgca cggcggagcg ggagaatagg tgccatgagt gacgacgctc
      721 ccaccaccga gtccggcagg atcccgaaca agggcaagct ggccaaggac ctcaacgagg
      781 tcatccgcta cacactgtgg tccgtcttca agctgaagga cgtgctcccc gaggaccgcg
      841 cggggtacgc cgacgaggtc caggagctgt tcgaccagct cgccgcgaag gacgtgacga
      901 tccgcggcac ctacgacctc tccggcctgc gcgccgacgc cgacctcatg atctggtggc
      961 acgccgagac cagcgaccag ctccaggagg cgtacaacct cttccgccgc acgaagctcg
     1021 gccgcgcgct ggaaccggtg tggtcgaaca tggcgctgca ccgccccgcg gagttcaacc
     1081 gctcgcacat cccggcgttc ctcgccgacg agacgccgcg caactacgtc agcgtctacc
     1141 cgttcgtgcg ctcctacgag tggtacctgc tggccgacga ggaccgccgc cgcatgctcg
     1201 ccgaccacgg caagatggcc cgcggcttcc cggacgtccg cgcgaacacg gtcgcgtcct
     1261 tctccctcgg cgactacgag tggctcctcg ccttcgaggc cgacgagctg caccgcatcg
     1321 tcgacctcat gcgccacctg cgcggctccg aggcgcggat gcacgtccgc gaggaggtcc
     1381 ccttcttcac cggccgccgc aagtcggtcg ccgatctggt cgccgggctg gcctagccac
     1441 gcccgcctag gcccccttgc ccttcagggc gtcccgcagg acctgccggt cggcggatcc
     1501 ggtggccggg ggctggggct gcggttccgg gtgcggcgcg caggcggcgt gctgctccgg
     1561 aaggcggccc tccagcaggt aggcgtccag gtgggcgttg acgcagcggt tggggccgcc
     1621 cccgatgccg tgcgagcccg cgtcccgctc ggtcaccagc accgagccgg acaggcgccg
     1681 gcgcatctcc agcgcgcccg cgtacggcgt ggccgcgtcc cgctccgccg ccaggatcag
     1741 caccggcggc agctcaccgg ggccggtccg cacgtccagc ggccgctggc ggggcgccgg
     1801 ccagtacgcg cacggcaggt tcatccacgc gttgtcccac gtctcgaagg gcgccacgcg
     1861 cgcgaggctg gtgttgtcgc ggtcccacac cgtccagtcc gtcggccagg gcgcgtcgtt
     1921 gcactcgacg gccgtgtaca ccgcgttgcc gttctccgcc tgggcggccg tctgcgcgtt
     1981 cggcgcggcc agccgcacca gtggcttttc gtcgccgcgc aggtaggccg acagcgcggc
     2041 ggcgcggccc ggccagaaca cgtcgtcgta cccggcggtg aggaacgcgg actgcagctc
     2101 gcccgggccc accttcccgc ccgccggctt cgcggccagc cggccgcgcg ccgtgtcgta
     2161 gccgcgctgc accgcctgcg gggtgccgcc cagccggtac acgtcgtcgt gccgggcgat
     2221 ccagtcccgg aagtccgccc agcggtcctc gaacgcggcc gactgctcca ggttgttgcg
     2281 gtaccagacc ttctgcgggt ccgggtcgac caccgagtcc agcaccatcc gccgcacgtg
     2341 cgaggggaac agcgtcgcgt acaccgcgcc gaagtaggtg ccgtacgacg cgcccatgaa
     2401 ggtcagccgg tcctcgccca gcgccgcccg caggacgtcc aggtcgcggg cgttgtcgag
     2461 cgaggtgtag aacctcagcc tgctccccga ccgttcggcg cagccccgcg cgtacgcctt
     2521 cgcccgggcg atgcgctgct gcttgtacgc ctccgagggg tgctccggcg actcggtggg
     2581 cgcctcgacg aactgcgcgg ggtccatgca ggacagcggc gccgagccgc ccactccgcg
     2641 cggtgcgtag ccgaccaggt cgtacgcggc ggcgatccgc ttccacgcgg gcagcttcgc
     2701 gacgagtggg aagcgcatcc cggagccgcc ggggccgccc gggttgtaga ccagcgcacc
     2761 ctgccggggc accttccgct tgctgttctg cgggtcctgc tgcgtggccc gcatccggct
     2821 gacggtcagc cggatccggg tgccgtcggg gtgcgcgtag tccagcggga cctccaccgt
     2881 gccgcaccgc agaccggccg gcgcctgcgg gatcgccgcg catgcgccga agcggacacc
     2941 ggccgccctg gcccgcgcgg cggccacggc ggtgccgcgc agctcggccg cgcccggggc
     3001 ggcgggcgcc gccgggccac cgggcgcacc gcccgccggg gccgcggcca gggtggtcag
     3061 cagcaaggac ccgacggccg agtacagggc ggcagctctc atcgcgtatc ccttcggtgc
     3121 gcggcggcgc tggcacggct gccacgcccg gcgatcacag aagggatgtt tcgtctccct
     3181 tcgggcgaag gcaagcaccg ccccgcggtg tcgccgccaa tgcctccgtg cgcccccggc
     3241 gcagggtcac tcccgcccgg gccggtcgcg gtgcgtgaag gccgcgcgca gctccggctc
     3301 gccgatggcg cggacgcccc ggacggcgac ggaggtgagg aaggtccgct cgtcgccgtc
     3361 gccggccggc ccccggtgca gcgcgcccag cagccgctgc ccggccgggg aggcccagcg
     3421 ggagtacggg tgcacctcgg cccgggcgat caccaggcag ctcagggtga gggcgagggg
     3481 cagcgcgaac cacagggcga ccagcaggcg cggcagcccg gtcggggcgg gcagcagcag
     3541 cgcggtcagg cccagcgcgc acacggccag cgcggcggcc cgcaccccgc ggaccccgtc
     3601 cgccaccgtc gtccgcgccc cgtcgggcac cgcgagcccg gcgcgcacca gcccgtcggc
     3661 gagccgccgt accgcctcgg cgccggccgc ggcggcccgc accggcgcga tgcgggactg
     3721 cccctccgga ccgatggccc ctatgacgga ccgctccatc tcgtcgcgcc cccgcgggtc
     3781 cacgaccgtg gcccagccgg tgtgtgcgag cagcagccgc cgctgccggg ccatggagac
     3841 catcgtcacg tcggcgaccc ggcgcggacc gccggacagg aacgccgcct cgtacagcgt
     3901 cagatcgcgc tcccggtgcg tcgcggcgcc ggcgtccgcg ccggctcgtg cggagtcggc
     3961 ggccgcggcg cgtacggcgg ccaggcacag ccgggtgcac gcggtgccgg cgaacgccca
     4021 ggccaggagc agcaggagga cccagaacat gacgtttttc tatgccaggg gtctgggaga
     4081 gcgccatgcc ctgttcacca tccggaccga ctgttgcagg taggtgacgt tccgttttct
     4141 ccgcaggtca gcgtgcggcc gacggcggcg gggtggaggc ggcgggcggc agcgggtacg
     4201 tcgcggacgg cgagggctcg accgggaccg cggcggacgg gtcgaccgac ggggggtccg
     4261 gcacggcgga gggggaggcg gccaggtccc gggccagcgc ggagaagtcg acgtacccgg
     4321 tcgcctccag gaccttgatg tggtccagca cggtggtgtt cgcgtcgtcg gccagatcgc
     4381 ggaccagcgc gttctgggtg ccggcgcgga cctgggcgac cagcgagaac acgcggccgt
     4441 gggcgagccg caggatgccc gcgaaccggc ggtcgaactc ctggccccgc gccgcccgca
     4501 gggtggccag ccactgcttc tgctgggcgt tgggctcgtt gggcaggggc acgcccagct
     4561 gcgtggcgac ggtgcggtcg tgggagtcga gggaggtgtg tccgtcgacg agatggcggc
     4621 ccgcgtcccg tacggcctgg gtggtgccct tccgctccgc catctccccc gccgggagct
     4681 cccacaggcc ggccagccgc accttggtga cgaagtcccg gtcctgtgcg gacagcgggc
     4741 cgtacggggt cgtcacggtc tgcgcgctga gcaggctcgc gctcgccgcg tcggccggcc
     4801 ggtcggcgta ggacaggatc gggaagagca gcgccgcggc tgtcgccgcg aggcccgcga
     4861 tgatcagtcc ggtgccgttg aacatgcctc ggcccttgac ggggggtcgc ggtcgcatgg
     4921 tgcctcctgc gcgcaccggg gcttcgggtg cgggcttggc atattactgg gggcgccgtg
     4981 ccgtcgttcc ccccggccgg tcaggctctg ccgatcagga cgcgggcccg ggcccgcagg
     5041 ccgggggagc ggggggccgg tcccgagcgg tcgagccacc aggtgcgcag ctgcctgcgg
     5101 gcccgggcgc cggccggccg gccgagcttg aggaggtggc cggcgaagtc cagggcgtcg
     5161 cggcggtacc cgtcggtcat cgggcgggtg cgcgcgtacg cgaggaacgc ctcccggtaa
     5221 ccctcgccga ggatcaccgg cagctcgggc gccaccttgg ccaccacgct cgcccgcttg
     5281 gcggccagcg cccgggcctg cacgcccatc cggacccggt cgaacccttc cggcaccggc
     5341 gtcccggcca ccaggcagga cagcgcggcg gcctgggcga ccgcgacgcg ctgccgggcg
     5401 ccgggccggg cgggggcggc gctgtcggcg gggccggccg tgccggccgc cgtccccgtc
     5461 gccgtaccgc cgtcgccggg cggccgtccc gccgcgacgg ccgcccggat ctcgtccagc
     5521 tcgcgctcca gctcggcggg cccggggaag ttctcgtccc gttccagcag cacgccgggc
     5581 ggcgccacgc gggacgccag gccggtcagg acgtccagga cggggcgcgg gacggggtgc
     5641 gcgtggctgt cgtgccagac gccgtcccgc tcgaagccgc cggcgacatg gacgtaggcg
     5701 agcgcttcca gcggcagccc ggccagcgcc tcggccgggt cctcgccccg gttgacgtgg
     5761 ttggtgtgca ggttggccac gtcgacgagc agccgcaccc cggtccgctc gaccagctcg
     5821 gacaggaact gagcctcggt cagctcctca cccggccagg agaacagcgc ggcgatgttc
     5881 tccagggcca gcggcaccgg cagcgcccgc tgggcgatcc ggacgttctc gcagagcacc
     5941 tcgagcgcgt cccgggtgcg cggcaccggc agcaggtggc cggcctccag gcgcggcgag
     6001 gcggtgaggg cgccgcccgc ccggacgaac gcgacgtgct ccgtgaccag cggtgccccg
     6061 agcgcctcca cccgctcggc gagcgccgcc agccgcccct cgtcgggccg ctccgcgccg
     6121 ccgaggccga gcgagacgcc gtgcgggatc acggtcaccc cgcgctcgcg cagccgcagc
     6181 agcgactcgg gcaggtgtcc cgggcacacg ttctccgcca cgacctccac ccagtcgatc
     6241 cccggcatgc cctccacggc gtccgcgatc tccggccgcc agccgatccc cgtccccagt
     6301 cgccgcatga ctccaccccc tcctcgaatg aggggggtat ggccccggcg gcccgccgcg
     6361 aaccgtcccg gtgcggcgtt cagagcaaca tttgagcttc gcccgggtgc gcatccgggc
     6421 aggcgcacgg gcaactcctg gcaggatgcc tgctcatggg gtctggaagc tggggcaggc
     6481 atgcggtcga gtcgaccggc gggcgggtgg tcaagcggtt cccgcacggg gacggtggcc
     6541 gcgccgcgcg tgagtggcgg gccctgacct tgctggcccg gcatgctccc gggctggccc
     6601 ccgagccgct tcaggccggt ctcgcggcgt ccgagccggt cgtggtcatg tcccggctcg
     6661 acgggaagcc cctgcgggga gcagcgctga gcggcgagca gatcgacgcc ctggccgagg
     6721 cggtggccgc cgtgcacgcg gcgctgccgt cccacgtcct ggacggggtc cctctccggc
     6781 ccgggcacca ggccgagctc gtcgggcacc tgcgtgcgtg ggcggggacg gctcgccccg
     6841 cggccgaggg tccggtgcgg caggccatgg acgcaggtct ggcctggctg gccgcatccg
     6901 gtctggaagc cgacggggcg tggggcgtgc cgccggtctt cggtcccggc gacggcaatc
     6961 tcgcgaacta cctttgggac ggccgccggg tccggatcgt cgacttcgag gagtccggcc
     7021 gcagcgaccg cgccttcgag ctggcggaga tcaccgagca cgtgggcagc tgggtggacg
     7081 gcccgctgga cgtccccgcg ttcctggacc ggttcgacct ctcgcccgtg gaggcggccc
     7141 ggttgcgcga gtgccgccgg ctggttgctc tggtctggtt gttcctgctg gcggccgacg
     7201 acccggacca ccccgggaat ccgccgggaa ccgcggagag gcaggccggc cgggtgctgg
     7261 acctgctggc ctgagggcgt gggccgacag cgcggaccgg gcggccccgg ggacgcggcc
     7321 gagccccgat tcatccgggc accgactcga acggatgcct ggcgcgggct cgggtacccg
     7381 gcttcgagta cggcccctgg ccgagtccgg tacgaggtga cagcaacgga gatgtagatg
     7441 aaggccccgc gatcagcagc cccgccggct cccgcgcaag cttcagcgga tccgctgtcc
     7501 gctcatgtcc tcgccggccg ctaccggttg ggcgcgcgga tcggctcggg aggcatggcc
     7561 gacgtctacg acggggtcga cacgcggctt cggcggcccg tcgccgtcaa ggtcttccgg
     7621 ccggggcccg acgcgcactg cgaggaccgg ctcgccgcgg aggccgtgct gctcgcgcgt
     7681 ctgcagcacc ccggcctggt caccgtctac gacgtcggcc ggtacgagga ccgcacctac
     7741 ctggtcatgc agctggtcga cgggcccacg ctgcgcagcc tcctggcggg cggaacgctg
     7801 cccgagcggc gcgtggccga actgggcgcc gccctcgccg gggcgctcgc gcacgtccac
     7861 cgggccggca tcgtgcaccg cgacgtcaag ccgtcgaacg tcctcgtcga cccggccggc
     7921 gccccccatc tggccgactt cggcatcgcc cgggtggcga acaccaccgt ccacacggca
     7981 cctgacgtgc tgataggcac cgccgcctat ctggcgcccg agcaggtcga gggcgggcgg
     8041 gtcgggccgg ccgccgacgt ctacgcgctc ggcctggtgc tgctggagtg cctgaagggg
     8101 gaactggagt accagggcac cgcggtggaa gtggcggtcg cccggctcca ccgcccgccc
     8161 acggtcccgg cgtggctgtc cccggacctg gcggccctgc tcggggccat gaccgcccgg
     8221 gatcccgagg cgcgcccgga cgccgggcag tgtgcgaggg agcttgcccg cctgtgctcc
     8281 gcggacggcg cgcacgcgct gcagcagctc ccggcacccg ccgaagcccg cgttccgcgc
     8341 gcccgtgccg ctcgtcatga ggcggacgac cccgtacgga cgcaggaggc gccggccgcc
     8401 cggaccggcg tcccgggcaa cgcccgcccg agccgtcgcc tggccgtcgg caccgccctg
     8461 gccgcgctct ccgtggccct gggcacgacc ctggccgtgg ccccgcgcac cagcggagcc
     8521 ggcgacgacc gcgccgccgc gccctcgtcc ccggcggcca ccggcagcag gcccggtacg
     8581 gtctcgccgg tccggcccct gcgggctcag ccctcctccg ggaccgtcga cgtgtcggat
     8641 ccggcggccg ggtcgagcgg ctcccacgcg gagcccgccg tccagcgcgt gaactctcct
     8701 ggcacgccgg actccacgta tcgggcaccg gccggacgcc acggcgccga gcaccgcaag
     8761 ccgtccccgc accgccccgc cggcacggac ggccgcccgc gtcacgccgc cccccacgga
     8821 ggcccgcacc acggcgctcc gcagaagggg aagggccacg gctgacggac ggccggcggc
     8881 tacgggcgcc cccgacgggg cagagaagcg aactgcccac cgccctccac cgcgacggtg
     8941 gtccccggtg cgatctccgt gaagccggcg tcccggacca acggcagtcc ggcgcccgtc
     9001 agttcggccc atcgggcagg ggcggcggtg cgtacggcga gcgggaagcc cgccttccgc
     9061 caggcggtgc gcgcctcctc ggacagtgcc caccaggcca gttgggcgcc gtgaccggcc
     9121 tgcgccatgg acttgccggc cgtcatcgtc agccccgggt tcagccacag caccggcgcg
     9181 gccgggtcgg cgccggcggg cggctcgggg tcgtccagct ccgtgccgga gacctgcagc
     9241 ctggccaggt ccttgggcca gccgtccagc gggaccggcg ggaagacccg cacctccgcg
     9301 gaccggccgg tcaccgtgat cccgggcagc gcctcggcgc gccgccactc ggcgccgcgc
     9361 gcccgccgca ccaccttgcg gatccgggcg tcctcccagc cgcgcacggc ctcggcccac
     9421 tcgccgtccc cggccgaccg ctcgtcgccg agcagcgtga gcacggctcg ggcggcggtc
     9481 tccagtgcgt cggtgcgggc gggcggagcg ctccgctcga tccgtacgac gagcggcaga
     9541 acgaactgcg gtgccgcgtc gcgcggggcg tgctcggagc ggaacgggct gtcggcgggg
     9601 gtggcgggaa cgtgggtcac ggaagtcaac cctacgagcc gccgccggga ccccggtagg
     9661 ccggtccggc gccgctcacc ccgagcacgc cgtgcgctgg gcctcctgcc actcgcacac
     9721 cgggcacagg gtgacgccct tgtacgactc cgggtattcc gtgggctcac ggcacagcac
     9781 acactcggcg tacggcggcc cgtcggcctt cgggggccgg gccgcgtcga cgatcatgca
     9841 gtagtcctcg tcgtccttct ccgtcatgcc ttcagcgtag gaccgttcag cgacggccgt
     9901 tcgtctccgc gatcagctcg gacaccttcg cgaagcggta gccccgcttg cgcagttccg
     9961 gcacgaccgc gcggacgacc cgctccgtcg tcggggcggc gctgcgggtg cagtgcatga
    10021 cgaccaccga gcccggcttc accccgttca gcacctgttc gacgacggcg tcggcgtccg
    10081 tggcgaaggc gtcgccgctg atcacgtccc actggacggc ggtgaccccg acgccctgca
    10141 gcgcgcgcag cgcctgccgg tcgtagcagc cgccggggaa gcggaagtac ggcagcggcc
    10201 ggggcacgcc cgcctggcgc agggcggcgt aggcccgctc cacgtcctgg cgcatgtggt
    10261 ccgcgccgac cgtcggcagg ccgtagcagt cggcggtgaa cgcgtagtgg ctgtaggagt
    10321 ggttggcgac ctcgaactgc gggtcgtggc cgagggagcg ggcctgggcc gggtactgct
    10381 cggcccagcg gccggtcatg aagatcgtgg ccgggacctt cagcgcgcgc agcgcgccga
    10441 tgaggtccgg gttgtcgaag tgctccccgg cggcggcacg gggcccctcg tcggcggtca
    10501 tgtcggcgtc gaaggtcagg gcgacggtct tgccgagggt gcgcgggccg tgctcgaaga
    10561 cgggggtcag gccgcccggg ccgggggcga gcacgggcgg ccgggtgggg gagggggagg
    10621 cggtgggctt ggcgggggcg tggtgggccg gttcgggggc gcccgacgta ccgcaggcgg
    10681 cgagggtcgc ggccagggcg caggcggcgg cgatacggcg tactggtggg atcaccgtac
    10741 gaacgtagga ccagacgtcc ggttgtatgt ctgtatgact cgtgtcgggt gggcacggtc
    10801 acccgcgcgg cggcggtccg ttccgggccg tcacaggtcc cgctgctcca gcggccgcac
    10861 cgccgggccc tggatcacgc tgccgtccac gtcgaagcgg gagccgtgac aagggcactc
    10921 ccaggcgcgt tcggcccggt tgaaggcgac caggcagccc aggtgggtgc agcgggccga
    10981 gacggcgtgc agttctccct cctcgtcgcg gtgcacggcg aggcgctcgt gcccggcccg
    11041 gaccaccgcc ccgtcgccgg gcgccaggtc gtccacggag cccggcggcc gcagccggtc
    11101 gccgacgaag tgccgggcca cctccgcctg ggtcctcagc agcgacggcg cctcgcgcag
    11161 caccgacttc agccggcgcg ggtcgtacag cccctgccac ggacgggtct gcccggtgat
    11221 cagcgcggtg agcagcagcc cggccatgat gccgccgctc agcccccagc cgccgaagcc
    11281 ggtcgcgacc caggcgtgcc gggcgccc
//
