LOCUS       NZ_KQ948851            12118 bp    DNA     linear   CON 21-APR-2024
DEFINITION  Streptomyces bungoensis strain DSM 41781 PRJNA299233_s001, whole
            genome shotgun sequence.
ACCESSION   NZ_KQ948851
VERSION     NZ_KQ948851.1
KEYWORDS    WGS; RefSeq.
SOURCE      Streptomyces bungoensis
  ORGANISM  Streptomyces bungoensis
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 1011152)
  AUTHORS   Ruckert,C., Winkler,A., Kalinowski,J., Kampfer,P. and Glaeser,S.
  TITLE     Draft genome sequence of Streptomyces bungoensis DSM 41781, type
            strain for the species Streptomyces bungoensis
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1011152)
  AUTHORS   Ruckert,C., Winkler,A., Kalinowski,J., Kampfer,P. and Glaeser,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2015) CeBiTec, Bielefeld University, Sequenz 1,
            Bielefeld 33615, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 13-OCT-2014
            Assembly Method                   :: Newbler v. 2.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 60.6x
            Sequencing Technology             :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_001514215.1-RS_2024_04_21
            Annotation Date                   :: 04/21/2024 02:15:15
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,539
            CDSs (total)                      :: 7,454
            Genes (coding)                    :: 7,226
            CDSs (with protein)               :: 7,226
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 1 (5S, 16S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            tRNAs                             :: 80
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 228
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 67 of 237
            Pseudo Genes (incomplete)         :: 196 of 237
            Pseudo Genes (internal stop)      :: 10 of 237
            Pseudo Genes (multiple problems)  :: 43 of 237
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 00:26:42
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 95221
            Orig. end                         :: 107339
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            KQ948851.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..12118
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="1"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..12118
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..12118
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:7118](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..7118
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            729..2201
                     /locus_tag="AQJ66_RS00330"
                     /old_locus_tag="AQJ66_00330"
     CDS             729..2201
                     /GO_function="GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA]"
                     /GO_process="GO:0042245 - RNA repair [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020125168.1"
                     /locus_tag="AQJ66_RS00330"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AQJ66_00330"
                     /product="3' terminal RNA ribose 2'-O-methyltransferase
                     Hen1"
                     /protein_id="WP_061914577.1"
                     /transl_table=11
                     /translation="MFLTISTRGTAALPATDLGYLLHKHPGKAQKFSTSYGTAHVFYPE
                     ADAERCTAALLLEVDPLALVRRGKGKGRGGAPDAALAQYVNDRPYAASSLLAVALSAVF
                     SSALRGVCAARPERAAAPLPLRVEVPALPARGGPDLVRGLFGPLGWDVTAEPVALDGEF
                     PEWGDSRYVRLVLESEALTLAEALRHLYVLLPVLDDAKHYWVSSDEVDKLLRAGDGWLA
                     GHPEQQLITSRYLSRRWSLTRAATERLELARLAEADDSQVEAIDNAVEPETGTEEKPTP
                     LAVRRRDAIIEALRACGAARVLDLGCGQGRLVQELLKDARFTEVAGVDVSVRALTIAAR
                     RLKLDRMGERQASRVQLFQGSLAYTDKRLKGYDAAVLSEVIEHLDLPRLPALEYAVFGH
                     ARPRTVLVTTPNVEYNVRWESLPAGHVRHGDHRFEWTREQFRTWAGAVAERHGYGVGFR
                     PVGPDDPEVGPPTQMAVFTLHTHANEKEAKAA"
     gene            2198..4744
                     /locus_tag="AQJ66_RS00335"
                     /old_locus_tag="AQJ66_00335"
     CDS             2198..4744
                     /GO_function="GO:0004113 - 2',3'-cyclic-nucleotide
                     3'-phosphodiesterase activity [Evidence IEA]"
                     /GO_function="GO:0051731 - polynucleotide
                     5'-hydroxyl-kinase activity [Evidence IEA]"
                     /GO_process="GO:0042245 - RNA repair [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020122488.1"
                     /locus_tag="AQJ66_RS00335"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AQJ66_00335"
                     /product="polynucleotide kinase-phosphatase"
                     /protein_id="WP_061914580.1"
                     /transl_table=11
                     /translation="MTDTARTGGRILPVTDLSLVVLVGASGSGKSTFARRHFKPTEVIS
                     SDFCRGLVSDDENDQSATGDAFDVLHYIAGKRLAAGRRTVVDATSVQQDARRQLIDLAK
                     RYDVLPIAIVLDVPEEVCAERNAARTDRADMPRRVIQRHIRELRRSLRHLEREGFRKVH
                     VLRGAEEAENATVVTEKRFNDLTHLTGPFDIVGDVHGCSAELESLLGKLGYTDGVHPEG
                     RTAVFVGDLVDRGPDSPGVLRRVMSMVKSGNALCVPGNHENKYGRHLKGRGVRHTHGLA
                     ETIAQMAGESEEFKAEVRQFIDGLVSHYVLDGGRLVVCHAGLPERYHGRTSGRVRSHAL
                     YGDTTGETDEFGLPVRYPWAEDYRGRAAVVYGHTPVPEATWLNNTICLDTGAVFGGKLT
                     ALRWPERELVDVPAERVWYEPAKPLRTEAPGGQDGRPLDLGDVHGRRVVETRHAGRVSV
                     REENAAAALEVMSRFAVDPRLLPYLPPTMAPTPTSRVDGYLEHPVEAFAQYAADGVAQV
                     VCEEKHMGSRAVALVCRDAEAARGRFGVDGPTGSLYTRTGRPFFDDGEVTEEILGRLRA
                     AIGAAGLWEELATDWLLLDAELMPWSLKASGLLRTQYAAVGAASGAVLPAALAALESAA
                     ARGTDLGGLLERQRERAADAAQFTAAYRRYCWSTEGLEGVRLAPFQILAARGRSLAALP
                     HDEQLALLDRLVEHDGTGLLRTTRRLRVDTTDPESVRAGVDWWLEMTGRGGEGMVVKPL
                     GALARTEQGRLVQPGVKCRGREYLRIVYGPEYTRPDNLAQLRRRFLNHKRSLALREYAL
                     GLEALDRLADGEPLWRVHEAVFGVLALESEPVDPRL"
     gene            4855..4998
                     /locus_tag="AQJ66_RS38710"
     CDS             4855..4998
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="AQJ66_RS38710"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_159052513.1"
                     /transl_table=11
                     /translation="MDDAQDVQDAQPQGAVTVVGMEIRKTVVREDRGTTRRPAILEKRP
                     TC"
     gene            5001..7118
                     /locus_tag="AQJ66_RS00340"
                     /old_locus_tag="AQJ66_00340"
     CDS             5001..7118
                     /GO_function="GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: TIGR03975"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF04055"
                     /gene_functions="other (smcogs) SMCOG1277: Radical SAM
                     domain protein"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014675582.1"
                     /locus_tag="AQJ66_RS00340"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AQJ66_00340"
                     /product="RiPP maturation radical SAM C-methyltransferase"
                     /protein_id="WP_167359134.1"
                     /sec_met_domain="TIGR03975 (E-value: 7.1e-166, bitscore:
                     548.8, seeds: 14, tool: rule-based-clusters)"
                     /sec_met_domain="PF04055 (E-value: 8.7e-12, bitscore: 41.5,
                     seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRLHLVTMPWQPLDLPSLQLGLLNRLVRRTRPGTEVTEFHGSLRW
                     AEFLLEHSAGALRPGDYVEVGSDAIFHGLGDWVFSGVLYDDPGWGLDRLRAYAADRDVP
                     VATALAMRPHAAAFTAACAAEILAAEPDVVGFTTTFMQNVSSLALARELKSRRPGLTVV
                     LGGSNCDGPMGHALHRNHPFVDHVVRGEAEYAFPALLRHLEAGTAPVDVPGLCWWDGPV
                     PRANTETRRTVAPADIPSPDYDQWQEALDASPVREFVHPKLVVEGARGCWWGEKHHCTF
                     CGLNGSAMAFRAKPGERLWAEVDRLVRRHRLLDVVTVDNILDMAHFRDFLPRAAASGWD
                     LRLHYEVKSNLTPDQLRLLADSGTVHIQPGIESLGSRVLDLMDKGVTAARNVRTLRECE
                     NHALTCSWNLLYGFPGETAEDYAPVVGQLAALVHLQPPSGAHRIQLERFSPHFTDLELG
                     FRKRRPARMYSHVYDLPESELADLVYLFDTDDAGIGGAAEQRLGEAVAAWRAGHAQSRL
                     LFEEDGADLLVHDRRHGWPAATHRFTGWRAAALRRLEDGRTVAALHRLLAADGHPVPAD
                     SLAEWVRHALALGLLFRDGGTYVALPTWGEPDRLPDGADGVLYHRRGPGFVTVMDRREP
                     GTAVRLTLDHPALVAAFDLLAEPTPLDTLDPVQREAVRLLTAERLVRVEAGRAVALPPR
                     IRRWPVPCTAI"
     gene            complement(7146..7397)
                     /locus_tag="AQJ66_RS00345"
                     /old_locus_tag="AQJ66_00345"
     CDS             complement(7146..7397)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014677115.1"
                     /locus_tag="AQJ66_RS00345"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AQJ66_00345"
                     /product="DUF5133 domain-containing protein"
                     /protein_id="WP_061914583.1"
                     /transl_table=11
                     /translation="MLLPAEKELRAVLARFADARFRHDLQPTGHSSRELEDTSYTLCVM
                     TGTQTVDEALAAADALLERMTADRRAVTTTRATQTLAA"
     gene            complement(7547..8446)
                     /locus_tag="AQJ66_RS00350"
                     /old_locus_tag="AQJ66_00350"
     CDS             complement(7547..8446)
                     /GO_function="GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014044102.1"
                     /locus_tag="AQJ66_RS00350"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AQJ66_00350"
                     /product="NAD-dependent protein deacetylase"
                     /protein_id="WP_061914586.1"
                     /transl_table=11
                     /translation="MRMRPTLTWTPTEDLPPGTTDLRPVADALGGGGVLVLSGAGISTE
                     SGIPDYRGEGGSLSRHTPMTYQDFTAGAQARRRYWARSHLGWRTFGRARPNPGHRAVAA
                     FERHGLLSGVITQNVDGLHQAAGSDGVVELHGSLDRVVCLSCGDVSPRRELARRLEEAN
                     PGFDPVAAGINPDGDADLTDDQVGDFRVVPCALCGGILKPDVVFFGEAVPPQRVEHCRA
                     LVREAASLLVLGSSLTVMSGLRFVRQAAEAGKPVLIVNRDPTRGDRHALTRVALPLGAA
                     LTAVAGRLGVSLDGGSAV"
     misc_feature    complement(8436..8449)
                     /note="TFBS match to ArgR, Regulator of arginine
                     biosynthesis genes, confidence: weak, score: 16.85"
                     /tool="antismash"
     misc_feature    complement(8459..8473)
                     /note="TFBS match to IolR, Regulator of the inositol
                     catabolism, confidence: medium, score: 19.12"
                     /tool="antismash"
     misc_feature    complement(8460..8473)
                     /note="TFBS match to LexA_variant_2, Repressor of DNA
                     damage response, confidence: weak, score: 17.24"
                     /tool="antismash"
     gene            complement(8522..8860)
                     /locus_tag="AQJ66_RS00355"
                     /old_locus_tag="AQJ66_00355"
     CDS             complement(8522..8860)
                     /GO_function="GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003981380.1"
                     /locus_tag="AQJ66_RS00355"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AQJ66_00355"
                     /product="STAS domain-containing protein"
                     /protein_id="WP_061914589.1"
                     /transl_table=11
                     /translation="MSPLAITTRDATTGPVLEIIGELDYANADELRERLTTLDLRPGQR
                     LVIDLSGMEFCDSSGLTALINARNHAHAAQADIALAAVPAHTLRVLRIVGLDQIFAVYP
                     DRDSAIGS"
     gene            complement(8857..10185)
                     /locus_tag="AQJ66_RS00360"
                     /old_locus_tag="AQJ66_00360"
     CDS             complement(8857..10185)
                     /GO_function="GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="other (smcogs) SMCOG1054: hypothetical
                     protein"
                     /gene_kind="other"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020120230.1"
                     /locus_tag="AQJ66_RS00360"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AQJ66_00360"
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="WP_107118822.1"
                     /transl_table=11
                     /translation="MMCPADQQPDPQDPDTGAGADAGAGRLADTASFAALLEDSAEDLY
                     ESAPCGYLSTLMDGTVAKINTTLLDWLGLEREAVVGRMRFTDLLTVGGRLYHETHFAPL
                     LRMQGELSGIALDVRRADGGRMPVLVSSAVKHGSTGEPLLIRTTLFDARERRAYEEELL
                     RGRKAAEEARRQAEADRTRLQEALAVLQQSLLPDSLPAVPGVETAAHYHTASPDRLGGD
                     FYDVFPVDGTRFAFFLGDVCGKGPQAAALTSLTRYTLRAAALHDPGPVAALTTLNKVLY
                     ERYSGSGDPRYCTAVYGTLEPDPATGRVAVHLAAGGHPPALVLRADGTAGYLPTPGGLL
                     VGVVPGAHFTTASATLGPGDTLLLYTDGLTEARTGDDRTGLYGDEALLAFATGLAGRSP
                     QAVVGALTGLLASFGDGLDDDTALLALGAPAHPAARPPRRGPA"
     gene            complement(10182..10985)
                     /locus_tag="AQJ66_RS00365"
                     /old_locus_tag="AQJ66_00365"
     CDS             complement(10182..10985)
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /NRPS_PKS="Domain: Abhydrolase_1 (17-251). E-value:
                     6.3e-26. Score: 83.4. Matches aSDomain:
                     nrpspksdomains_AQJ66_RS00365_Abhydrolase_1.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF00561"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Abhydrolase_6"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1036: alpha/beta hydrolase fold protein"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020130118.1"
                     /locus_tag="AQJ66_RS00365"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AQJ66_00365"
                     /product="alpha/beta hydrolase"
                     /protein_id="WP_061914595.1"
                     /sec_met_domain="PF00561 (E-value: 8.5e-23, bitscore: 77.0,
                     seeds: 48, tool: rule-based-clusters)"
                     /sec_met_domain="Abhydrolase_6 (E-value: 9.2e-24, bitscore:
                     81.0, seeds: 455, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MDVTRRNNVTVTGDPQGPAVVLAHGFGCDQNMWRATVPALARDHR
                     VVLFDYVGSGRSDPSAFSEKRYASLDGYARDAVEVCEALDLRDAVFVGHSVSAMIGVLA
                     ARTAPERIGALVMVAPSPRYLDDDGYRGGFSAADIDELLASLESNYLGWSAAMAPVIMG
                     NPDRPELGDELRNSFCATDPDMARVFARTTFLSDSRDDLKSVRVPTLVLECTQDVIAPR
                     EVGAFVHAAIPGSTLVTLDATGHCPHLSAPEATNEAITTFLAGLR"
     aSDomain        complement(10233..10934)
                     /aSDomain="Abhydrolase_1"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_AQJ66_RS00365_Abhydrolase_1.1"
                     /evalue="6.30E-26"
                     /label="AQJ66_RS00365_Abhydrolase_1.1"
                     /locus_tag="AQJ66_RS00365"
                     /protein_end="251"
                     /protein_start="17"
                     /score="83.4"
                     /tool="antismash"
                     /translation="PAVVLAHGFGCDQNMWRATVPALARDHRVVLFDYVGSGRSDPSAF
                     SEKRYASLDGYARDAVEVCEALDLRDAVFVGHSVSAMIGVLAARTAPERIGALVMVAPS
                     PRYLDDDGYRGGFSAADIDELLASLESNYLGWSAAMAPVIMGNPDRPELGDELRNSFCA
                     TDPDMARVFARTTFLSDSRDDLKSVRVPTLVLECTQDVIAPREVGAFVHAAIPGSTLVT
                     LDATGHCPHLSA"
     misc_feature    complement(11441..11450)
                     /note="TFBS match to AbrC3, Antibiotic production
                     activator, confidence: strong, score: 18.16"
                     /tool="antismash"
     misc_feature    11723..11737
                     /note="TFBS match to IolR, Regulator of the inositol
                     catabolism, confidence: medium, score: 19.19"
                     /tool="antismash"
ORIGIN
        1 tcgaactcgg cctcggcgcg ggctggttcg aggacgagca caaggcgtac ggcatcccgt
       61 tcccccagga gaagttcggg cgcctggagg agcagctcga gatcgtcacc ggtctgtggt
      121 cgaccaagct cggcgagacc ttcgacttcc acggcacgta ctacgacctg accggctcgc
      181 ccgcgctccc caagcccgcc caggaccgcg tccccgtcct gatcggcggg cacggcgcga
      241 ctcgcacccc gcgcctcgcg gcccggtacg ccgacgagtt caacatgccc ttcgcgtctg
      301 tggaggacag cgagcggcag ttcggccgcg tccgtgccgc cgccgagcag gcgggccggg
      361 gcgcggacgc cctgacgtac tccaacgcgc tcgtggtgtg cgtcggccgg gacgaccggg
      421 aggtcgcccg gcgggccgcc gcgatcggcc gcgaggtgga cgagctgaag gccaacgggc
      481 tggccggctc cccggccgag gtggtcgaca agatcggccg ctacgccgcg atcggctccc
      541 gccgcctcta cctgcagatg ctcgacctgc acgacctgga ccacctggag ctcatctcct
      601 ccgaggtcca gacccagctg cggtagcgcc cacgcgcgac gacggacccg cgcccccgtc
      661 ccggggcgcg gggccgtgaa ccggaaaacg ggtggtcacc gggggtcgcg gccgacagac
      721 tcggacacgt gttcctgacg atctccacac gcggcaccgc cgccctcccg gccaccgacc
      781 tcgggtacct gctgcacaag catcccggca aggcgcagaa gttctcgacg tcctacggca
      841 ccgcccacgt cttctacccg gaggcggacg ccgagcgctg cacggcggcg ctgctgctgg
      901 aggtcgatcc gctggcgctg gtgcgccggg gcaagggcaa gggccgcggc ggagcacccg
      961 acgcggcact cgcgcagtac gtcaacgacc ggccctacgc ggcctcctcg ctgctcgcgg
     1021 tcgcgctgag cgccgtgttc tccagcgcgc tgcggggtgt gtgcgccgcg cgcccggaac
     1081 gggccgccgc gcccctgccg ttgcgcgtcg aggtgcccgc gctgcccgcc cggggcggcc
     1141 cggacctcgt acggggcctc ttcgggccgc tcggctggga cgtgaccgcc gaaccggtcg
     1201 ccctcgacgg cgagttcccc gagtggggcg actcccgcta cgtacggctc gtcctggagt
     1261 cggaggcgct gaccctcgcc gaggcgctgc gccacctgta cgtgctgctg cccgtcctcg
     1321 acgacgccaa gcactactgg gtctcctccg acgaggtcga caagctgctg cgggccggcg
     1381 acggctggct ggccgggcac ccggagcagc agctcatcac cagccgctac ctctcccgcc
     1441 gctggtcgct gacccgggcg gcgacggagc ggctggagct ggcgcgcctg gccgaggccg
     1501 acgacagcca ggtcgaggcc atcgacaacg cggtcgagcc ggagaccggg accgaggaga
     1561 agccgacgcc gctcgccgtg cggcgccggg acgcgatcat cgaggcgctg cgcgcctgcg
     1621 gcgcggcccg cgtcctcgac ctcggctgcg ggcagggccg gctggtgcag gagctgctca
     1681 aggacgcgcg gttcaccgag gtcgccggtg tcgacgtgtc cgtgcgggcc ctcaccatcg
     1741 ccgcgcgcag gctgaagctg gaccgcatgg gggagcggca ggcgtcccgc gtgcagctct
     1801 tccagggctc gctcgcctac accgacaagc ggctcaaggg ctacgacgcc gccgtgctca
     1861 gcgaggtgat cgagcacctg gacctgcccc ggctgcccgc cctggagtac gccgtgttcg
     1921 ggcacgcccg cccgcgcacg gtcctcgtga ccaccccgaa cgtcgagtac aacgtccgct
     1981 gggagtccct accggccggg cacgtccggc acggcgacca ccgcttcgag tggacccgcg
     2041 agcagttccg gacctgggcg ggcgccgtgg ccgaacgcca cggctacggc gtcggcttcc
     2101 ggcccgtggg gccggacgac ccggaagtcg ggccgcccac gcagatggcc gtgttcaccc
     2161 tgcacaccca cgcgaacgag aaggaggcga aggcggcatg accgacacag cacggaccgg
     2221 gggacggatc ctccccgtca ccgacctctc cctcgtcgtc ctcgtcggcg cctccggctc
     2281 cggcaagtcc accttcgccc gccgccactt caagcccacc gaggtgatct cctccgactt
     2341 ctgccgcggc ctggtctcgg acgacgagaa cgaccagagc gccaccgggg acgccttcga
     2401 cgtcctgcac tacatcgcgg gcaagcgcct cgcggccggc cgccgcacgg tcgtggacgc
     2461 caccagcgtg cagcaggacg cccggcggca gctgatcgac ctggccaagc ggtacgacgt
     2521 gctgcccatc gccatcgtgc tggacgtccc ggaggaggtg tgcgccgagc gcaacgcggc
     2581 ccgcaccgac cgcgccgaca tgccgcgccg ggtgatccag cgccacatcc gcgaactgcg
     2641 ccgctccctg cggcacctgg agcgcgaggg cttccgcaag gtgcacgtgc tgcgcggagc
     2701 ggaggaggcc gagaacgcca ccgtcgtcac cgagaagcgg ttcaacgacc tgacccacct
     2761 caccggcccc ttcgacatcg tcggcgacgt ccacggctgc tccgccgaac tggagtcgct
     2821 gctcggcaag ctcgggtaca ccgacggcgt ccaccccgag ggccgcacgg ccgtcttcgt
     2881 cggcgacctc gtcgaccgcg gccccgacag ccccggcgtg ctgcggcgcg tgatgtcgat
     2941 ggtgaagtcg ggcaacgcgc tgtgcgtgcc cggcaaccac gagaacaagt acggccgtca
     3001 cctcaagggc cggggcgtcc ggcacaccca cggcctcgcc gagaccatcg cgcagatggc
     3061 gggcgagagc gaggagttca aggccgaggt ccggcagttc atcgacggcc tggtcagcca
     3121 ctacgtcctc gacggcggcc ggctggtcgt ctgccacgcc ggcctgccgg agaggtacca
     3181 cggccgcacc tcgggccggg tgcgctcgca cgccctgtac ggcgacacca ccggcgagac
     3241 cgacgagttc gggctgccgg tgcgctaccc gtgggcggag gactaccgcg gccgggcggc
     3301 ggtcgtctac ggccacaccc cggtgccgga ggccacctgg ctgaacaaca ccatctgcct
     3361 ggacaccggc gcggtcttcg gcggcaagct caccgcgctg cgctggcccg agcgcgagct
     3421 ggtcgacgta ccggccgagc gggtctggta cgagccggcg aagccgctgc gcaccgaggc
     3481 gccgggcggt caggacggcc gcccgctgga cctcggcgac gtgcacggac gcagggtcgt
     3541 cgagacccgg cacgcgggac gggtctcggt gcgcgaggag aacgcggcgg ccgccctgga
     3601 ggtcatgagc cgcttcgccg tcgacccgcg cctgctgccg tacctgccgc cgaccatggc
     3661 gccgacgccg acctcgcggg tcgacggcta cctggagcac ccggtggagg cgttcgcgca
     3721 gtacgcggcc gacggggtgg cgcaggtggt gtgcgaggag aagcacatgg gctcgcgggc
     3781 cgtggccctg gtgtgccggg acgcggaggc ggcgcgcggg aggttcggcg tggacggccc
     3841 gaccgggtcg ctctacaccc gtaccggccg gccgttcttc gacgacggcg aggtgaccga
     3901 ggagatcctc ggccggctgc gcgcggcgat cggggcggcc ggtctgtggg aggaactcgc
     3961 caccgactgg ctgctgctgg acgccgagct gatgccgtgg tcgctgaagg cctcggggct
     4021 gctgcgcacc cagtacgcgg ccgtgggcgc cgcgtccggc gcggtgctcc cggccgcgct
     4081 cgccgcgctg gagtcggcgg cggcccgggg caccgacctg ggcggcctgc tggagcggca
     4141 gcgcgagcgc gccgccgacg cggcccagtt caccgccgcc taccgccgct actgctggag
     4201 caccgagggc ctggaagggg tccgccttgc ccccttccag atcctcgccg cgcggggccg
     4261 cagcctcgcc gcgctgccgc acgacgagca gctggccctg ctcgaccggc tggtggagca
     4321 cgacggcacc ggactgctgc ggacgacccg gcggctgcgc gtcgacacga ccgaccccga
     4381 gtcggtccgc gccggcgtcg actggtggct ggagatgacc ggccggggcg gcgagggcat
     4441 ggtcgtcaag ccgctcggcg cgctggcccg caccgagcag gggcggctgg tgcagcccgg
     4501 ggtcaagtgc cggggccgcg agtacctgcg gatcgtctac ggccccgagt acacccgccc
     4561 ggacaacctc gcccagctcc gccgccggtt cctgaaccac aagcggtcgc tggcgctgcg
     4621 cgagtacgcc ctcggcctgg aggccctgga ccggctcgcg gacggggagc cgctgtggcg
     4681 ggtccacgag gcggtgttcg gggtgctcgc cctggagtcg gagccggtcg accccaggtt
     4741 gtagcccgcc ggcggccgct cttcacggaa cgcccttgtg ccgcacacgg atacgtccat
     4801 agggtggacg gccgtgcggc acgggggtgt ccgcgcaccc ggggaggggg gaccgtggac
     4861 gacgcgcagg acgtgcagga cgcgcagccg cagggggcgg tgacggtggt gggcatggag
     4921 atcaggaaga ccgtggtccg cgaggaccgc ggcaccaccc gccggccggc gatcctggag
     4981 aagcggccga cctgctgacc atgcgcctcc acctggtcac gatgccctgg cagccgctcg
     5041 acctgccgtc cctgcaactg ggtctgctga accggctggt gcgccggacc cggcccggga
     5101 ccgaggtgac cgagttccac ggctcgctgc gctgggcgga gttcctgctg gagcactccg
     5161 cgggcgcgct ccggccgggc gactacgtgg aggtcggcag cgacgcgatc ttccacgggc
     5221 tcggcgactg ggtcttctcc ggcgtcctgt acgacgaccc cggctggggc ctggaccggc
     5281 tgcgcgccta cgcggccgac cgggacgtcc ccgtagccac cgccctcgcc atgcgccccc
     5341 acgcggccgc gttcaccgcc gcctgcgccg ccgagatcct ggcggccgag ccggacgtcg
     5401 tggggttcac caccacgttc atgcagaacg tctcctccct cgccctcgcc cgtgaactca
     5461 agtcccgcag gcccgggctg acggtggtcc tcggcggcag caactgcgac gggccgatgg
     5521 gccacgccct gcaccgcaac cacccctttg tcgaccacgt ggtgcgcggc gaggcggagt
     5581 acgccttccc ggccctcctg cggcacctgg aggcggggac cgcgcccgtg gacgtgcccg
     5641 gcctgtgctg gtgggacggg cccgtgccgc gggccaacac cgagacccgg cggaccgtgg
     5701 cgcccgccga catcccctcg cccgactacg accagtggca ggaggccctg gacgcctccc
     5761 cggtgcggga gttcgtccac cccaagctgg tcgtcgaggg cgcccggggc tgctggtggg
     5821 gcgagaagca ccactgcacc ttctgcgggc tcaacggctc cgccatggcc ttccgggcca
     5881 aaccggggga gcggctgtgg gcggaggtcg accggctggt ccgccggcac cggctgctcg
     5941 acgtggtcac cgtcgacaac atcctcgaca tggcccactt ccgggacttc ctgccccgcg
     6001 cggccgcgag cggctgggac ctgcggctgc actacgaggt caagtccaac ctcacccccg
     6061 accagctcag gctcctcgcc gactccggca ccgtccacat ccagccgggc atcgagagcc
     6121 tgggcagccg ggtcctggac ctcatggaca agggcgtcac cgcggcccgc aacgtccgga
     6181 ccctgcgcga gtgcgagaac cacgcgctga cctgctcctg gaacctgctg tacggcttcc
     6241 ccggcgagac ggcggaggac tacgcacccg tcgtcggcca gctggccgcc ctcgtccacc
     6301 tccagccgcc gagcggcgcc caccgcatcc agctggagcg cttcagcccg cacttcaccg
     6361 acctggagct gggcttccgg aagcgccggc cggcccggat gtacagccac gtctacgacc
     6421 tgcccgagag cgaactcgcg gacctggtct acctgttcga caccgacgac gcggggatcg
     6481 ggggagccgc ggagcagcgg ctgggggagg ccgtcgccgc ctggcgggcc gggcacgcgc
     6541 agtcccggct gctgttcgag gaggacggcg cggacctgct ggtgcacgat cgccggcacg
     6601 gctggccggc cgccacccac cggttcaccg gctggcgggc cgcggccctg cgccgcctgg
     6661 aggacggccg cacggtggcc gccctgcacc ggctgctcgc cgccgacggc cacccggtcc
     6721 cggcggactc actggccgag tgggtgcggc acgcgctggc gctggggctg ctgttccgcg
     6781 acggcggcac ctacgtcgcc ctgcccacct ggggcgagcc ggaccggctg ccggacggcg
     6841 ccgacggcgt cctctaccac cggcgcggtc ccggcttcgt caccgtcatg gaccgcaggg
     6901 agcccggcac ggcggtccgg ctcaccctgg accatcccgc tctcgtcgcc gccttcgacc
     6961 tgctcgccga gccgaccccg ctcgacacgc tggaccccgt ccagcgggag gccgtacggc
     7021 tgctcacggc cgagcgcctg gtgcgggtgg aggccggccg ggcggtcgcc cttcccccgc
     7081 gcatccggcg ctggccggtc ccctgcacgg cgatctgacc tgaacgtccc gggacgcggg
     7141 ggcggtcagg cggccagggt ctgcgtcgcc ctggtggtgg tgaccgccct gcggtccgcc
     7201 gtcatccgct ccagcagggc gtcggcggcg gccagggcct cgtccaccgt ctgggtgccg
     7261 gtcatgacgc acagcgtgta ggaggtgtcc tcgagctctc ggctcgagtg gccggtgggc
     7321 tgcaggtcgt ggcggaaacg ggcgtcggcg aagcgggcga gcaccgcacg cagttccttc
     7381 tccgcaggca gcaacacagc gacaacctcc ctcgtcggac gggccgtcga cgcggcccgc
     7441 acatcaacgc agcgcgcacc cacggggtgg cacgacagtc gacacggagc agatggatgc
     7501 tccgtgcatc cgtgtgcccc gccaggctca cgtcatgcct ggcgtctcag acggctgacc
     7561 caccgtcaag ggagacgccc agccggcccg ccacggcggt gagggccgcg ccgaggggga
     7621 gcgcgacgcg ggtgagggcg tgccggtcgc cccgggtcgg gtcccggttg acgatcagca
     7681 ccggcttgcc ggcctcggcc gcctggcgga cgaaccggag ccccgacatc accgtcagcg
     7741 aggagcccag gaccagcagc gaggccgcct cgcggaccag cgcgcggcag tgctcgaccc
     7801 gctgcggcgg aacggcctcg ccgaagaaca ccacgtccgg tttgaggatg ccgccgcaga
     7861 gcgcgcaggg caccacgcgg aagtccccga cctggtcgtc ggtgaggtcg gcgtcgccgt
     7921 ccgggttgat gccggcggcc accggatcga agcccgggtt ggcctcctcc agccgccggg
     7981 cgagctcccg gcgcgggctg acgtcgccgc aggagaggca gacgacccgg tccaggctcc
     8041 cgtggagttc cacgacgccg tcgctgccgg cggcctggtg caggccgtcg acgttctggg
     8101 tgatcacacc cgagagcagg ccgtgccgct cgaacgcggc cacggcccgg tgcccggggt
     8161 tggggcgggc ccggccgaag gtgcgccagc cgaggtggct gcgcgcccag taccggcgcc
     8221 gggcctgggc accggcggtg aagtcctggt aggtcatcgg ggtgtgccgg ctcaggctgc
     8281 cgccctcgcc ccggtagtcg gggatgcccg actccgtgga gatgcccgcc ccgctgagca
     8341 ccagcacccc gccgccgccc agcgcgtcgg cgaccggccg caggtcggtg gtgccgggcg
     8401 gcaggtcctc ggtgggcgtc caggtcaggg tggggcgcat gcgcatgccg ccagggtacg
     8461 gaacgggtgt tccggccgcc ttgccgctcg cgctcatcgg ttcaccgccc cgggcaccgc
     8521 ttcaggagcc gatcgcgctg tcccggtcgg gatagaccgc gaagatctgg tcgaggccga
     8581 cgatgcgcag cacgcgcagg gtgtgggccg ggaccgcggc gagcgcgatg tcggcctggg
     8641 cggcgtgtgc gtggttgcgg gcgttgatca gggcggtgag gccgctggag tcgcagaact
     8701 ccatgccgga caggtcgatg acgaggcgct gccccggccg gaggtcgagt gtggtgagcc
     8761 gctcgcgcaa ctcgtcggcg ttggcgtagt cgagttcgcc gatgatctcc aggacgggac
     8821 cggtcgtggc gtctcgggtg gtgatcgcca gcgggctcat gcggggcctc tcctcggagg
     8881 gcgggcggcg gggtgggcgg gggcaccgag ggcgagcagg gcggtgtcgt cgtcgaggcc
     8941 gtcgccgaag gaggccagca ggccggtcag ggcccccacg acggcctgcg gggaccggcc
     9001 cgccaggccg gtggcgaagg cgagcagggc ctcgtccccg tacaggcccg tacggtcgtc
     9061 gccggtgcgg gcctcggtga gcccgtcggt gtagagcagc agggtgtcgc cggggccgag
     9121 ggtggcgctg gcggtggtga agtgcgcgcc gggcacgacg ccgacgagga ggccgccggg
     9181 ggtgggcagg tagccggccg tgccgtcggc gcgcaggacg agggccgggg ggtgcccgcc
     9241 cgcggcgagg tggacggcga cccggccggt cgcggggtcg ggttcgagcg tgccgtagac
     9301 ggcggtgcag tagcgcgggt cgccgctgcc cgagtagcgc tcgtagagca ccttgttcag
     9361 ggtggtcagg gcggcgacgg ggccggggtc gtgcagggcg gcggcgcgca gggtgtagcg
     9421 ggtcagcgag gtgagcgcgg cggcctgggg gcccttgccg cacacgtcgc cgaggaagaa
     9481 ggcgaagcgt gtgccgtcga cggggaagac gtcgtagaag tcgccgccga gccggtcggg
     9541 ggaggccgtg tggtagtggg cggccgtctc caccccgggg acggccggca ggctgtcggg
     9601 cagcagcgac tgctggagta cggcgagggc ctcctgcagc cgggtgcggt cggcctccgc
     9661 ctgccggcgc gcctcctcgg ccgccttgcg gccgcgcagg agctcctcct cgtaggcgcg
     9721 gcgctcccgg gcgtcgaaga gggtggtgcg gatcagcagg ggttcgccgg tgctgccgtg
     9781 cttgaccgcc gaggacacca gcaccggcat ccggccgccg tcggcccgcc tgacgtccag
     9841 ggcgatcccg ctgagctcgc cctgcatccg cagcagcggc gcgaagtgcg tctcgtggta
     9901 cagcctgccg cccacggtca gcaggtcggt gaagcgcatc cggccgacca ccgcctcgcg
     9961 ttccaggccg agccagtcca gcagcgtggt gttgatcttc gcgacggtgc cgtccatcag
    10021 cgtggacagg tacccgcacg gcgcggactc gtacaggtcc tcggcgctgt cctccagcag
    10081 tgcggcgaac gacgccgtgt ccgcgagcct gccggccccg gcgtcggccc cggcgccggt
    10141 gtcggggtcc tgcgggtccg gctgctggtc ggccgggcac atcatcgcag gcccgcgagg
    10201 aaggtggtga tcgcctcgtt ggtggcctcg ggcgcggaca ggtgcgggca gtggccggtc
    10261 gcgtcgaggg tgaccagggt cgagccgggt atcgccgcgt ggacgaaggc gccgacctcc
    10321 cggggcgcta tgacgtcctg ggtgcattcc aggaccaggg tgggcacccg cacgcttttg
    10381 aggtcgtcgc gggagtccga caggaacgtg gtccgggcga agacgcgcgc catgtccggg
    10441 tcggtggcgc agaagctgtt cctcagttcg tcgcccagtt cgggccggtc cgggttcccc
    10501 atgatcaccg gtgccatcgc ggccgaccag cccaggtagt tcgactccag ggacgccagc
    10561 agctcgtcga tgtcggccgc gctgaacccg ccccggtagc cgtcgtcgtc gaggtagcgc
    10621 ggcgacggcg ccaccatgac cagcgcgccg atccgctccg gggccgtgcg ggccgcgagg
    10681 acaccgatca tcgcgctgac ggagtgcccg acgaacaccg cgtcgcgcag gtcgagcgcc
    10741 tcgcacacct ccaccgcgtc gcgggcgtac ccgtccaggg aggcgtaacg cttctccgag
    10801 aacgcggacg ggtcggagcg gcccgacccc acgtagtcga agagcacgac ccggtggtcc
    10861 cgggccaggg cgggcaccgt cgcccgccac atgttctggt cgcagccgaa cccgtgggcc
    10921 agcaccaccg ccggcccctg cgggtcgccg gtgacggtga cgttgttcct gcgggttaca
    10981 tccataccgg ccagtctctc aggccccggc cggtactccg gcacggcgcc gccccgccgg
    11041 gcgcggcccg gtgcccggcc ccgcccggac gggcaccggc gaaaaccgcc gagaggggcg
    11101 tgaagggggg cggctgcggc caggatggag gcatggcata ccacgtcgac tccgaggccg
    11161 ggcggctgcg ccgggtgatc ctgcaccggc ccgacctcga gctcaaaagg ctcacgccca
    11221 gcaacaagga cgcccttctc ttcgacgacg tgctgtgggt gcgccgggcg cgcgccgagc
    11281 acgacgggtt cgccgacgtc ctgcgcgacc gcggggtcgc ggtccacctc ttcggcgacc
    11341 tgctcaccga gaccctggag atccccgagg cccgcgccct cgtcctcgac cgggtcttcg
    11401 acgagaagga gtacggcccg ctcgccaccg acccgctgcg ggccgccttc gagaagctgc
    11461 ccgcccggga gctgtccgag gccctggtcg gcgggatgac caagcgggag ttcctgcagg
    11521 ggcacgcgga gccgacctcc gtccggttcc acgtcatgga cctcgacgac ttcctgctgc
    11581 ggcccctgcc caaccacctc ttcacccgcg acacctccgc ctggatctac gacggggtcg
    11641 ccatcaacgc catgcgcctg cccgcccggc agcgcgagac cgtgcacttc gaggcgatct
    11701 accggcacca cccgctcttc cgcgacgaga cgttccgcgt ctggtcccag gggcaggccg
    11761 accacccctc caccatcgag ggcggcgacg tgctggtgat cggcaacggc gccgtgctca
    11821 tagggatgag cgagcgcacc accccgcagg ccgtggagat gctggcgcac aagctgttcg
    11881 cggcgggctc cgcgcgcacc atcgtcgcgc tcgacatgcc caagcggcgc gccttcatgc
    11941 acctcgacac ggtgatgacc atggtcgacg gcgacacctt cacccagtac gccgggctcg
    12001 gcatgctgcg ctcgtacacc atcgagccgg gggtcggcga gcgggaactg aaggtcaccg
    12061 accatccgcc ggagcacatg caccgcgcga tcgccgccgc gctcggcctc ggggagat
//
