LOCUS       NZ_KQ948009             3838 bp    DNA     linear   CON 21-APR-2024
DEFINITION  Streptomyces curacoi strain DSM 40107 PRJNA299217_s026, whole genome
            shotgun sequence.
ACCESSION   NZ_KQ948009
VERSION     NZ_KQ948009.1
KEYWORDS    WGS; RefSeq.
SOURCE      Streptomyces curacoi
  ORGANISM  Streptomyces curacoi
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 3838)
  AUTHORS   Ruckert,C., Winkler,A., Kalinowski,J., Kampfer,P. and Glaeser,S.
  TITLE     Draft genome sequence of Streptomyces curacoi DSM 40107, type strain
            for the species Streptomyces curacoi
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3838)
  AUTHORS   Ruckert,C., Winkler,A., Kalinowski,J., Kampfer,P. and Glaeser,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2015) CeBiTec, Bielefeld University, Sequenz 1,
            Bielefeld 33615, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 13-OCT-2014
            Assembly Method                   :: Newbler v. 2.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 65.7x
            Sequencing Technology             :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_001513975.1-RS_2024_04_21
            Annotation Date                   :: 04/21/2024 02:05:31
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,531
            CDSs (total)                      :: 7,455
            Genes (coding)                    :: 7,251
            CDSs (with protein)               :: 7,251
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 204
            CDSs (without protein)            :: 204
            Pseudo Genes (ambiguous residues) :: 0 of 204
            Pseudo Genes (frameshifted)       :: 91 of 204
            Pseudo Genes (incomplete)         :: 154 of 204
            Pseudo Genes (internal stop)      :: 21 of 204
            Pseudo Genes (multiple problems)  :: 54 of 204
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 08:06:44
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 0
            Orig. end                         :: 3838
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            KQ948009.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     gene            <1..>2552
                     /locus_tag="AQI70_RS36780"
                     /old_locus_tag="AQI70_36780"
     source          1..3838
                     /country="Argentina: Curacoi"
                     /culture_collection="DSM:40107"
                     /db_xref="taxon:146536"
                     /isolation_source="soil"
                     /mol_type="genomic DNA"
                     /organism="Streptomyces curacoi"
                     /strain="DSM 40107"
                     /submitter_seqid="PRJNA299217_s026"
                     /type_material="type strain of Streptomyces curacoi"
     region          1..3838
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="NRPS"
                     /region_number="1"
                     /rules="cds(Condensation and (AMP-binding or A-OX) and not
                     alpha_am_amid)"
                     /tool="antismash"
     cand_cluster    1..3838
                     /SMILES=""
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="cds(Condensation and (AMP-binding or
                     A-OX) and not alpha_am_amid)"
                     /kind="single"
                     /product="NRPS"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..3838
                     /aStool="rule-based-clusters"
                     /category="NRPS"
                     /contig_edge="True"
                     /core_location="[4:>2552](+)"
                     /cutoff="20000"
                     /detection_rule="cds(Condensation and (AMP-binding or A-OX)
                     and not alpha_am_amid)"
                     /neighbourhood="20000"
                     /product="NRPS"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5..>2552
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="cds(Condensation and (AMP-binding or A-OX)
                     and not alpha_am_amid)"
                     /neighbourhood="20000"
                     /product="NRPS"
                     /protocluster_number="1"
     CDS             5..>2552
                     /NRPS_PKS="Domain: Condensation_DCL (1-264). E-value:
                     2.1e-79. Score: 258.7. Matches aSDomain:
                     nrpspksdomains_AQI70_RS36780_Condensation_DCL.1"
                     /NRPS_PKS="Domain: AMP-binding (428-821). E-value: 4e-105.
                     Score: 343.7. Matches aSDomain:
                     nrpspksdomains_AQI70_RS36780_AMP-binding.1"
                     /codon_start=3
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     Condensation"
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     AMP-binding"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1002: AMP-dependent synthetase and ligase"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /locus_tag="AQI70_RS36780"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AQI70_36780"
                     /product="amino acid adenylation domain-containing protein"
                     /protein_id="WP_159042776.1"
                     /sec_met_domain="Condensation (E-value: 5.5e-51, bitscore:
                     160.4, seeds: 42, tool: rule-based-clusters)"
                     /sec_met_domain="AMP-binding (E-value: 7.4e-122, bitscore:
                     393.9, seeds: 400, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MVEAFAGAWQRVVDAVQALRVGVVWEGVAEPVRVVHRSVTLPVEV
                     VDWRGAGAEDVGARWDALVVADRERGLDLRQVPLARLTLARVGDERVRVLFTFHHVLLD
                     GWSLARVLSLVLDHHTARQRGTQPPAIVDRGLADHVRWLADRDLPAAEKYWRSVLSGYD
                     TPVDLPADRPLGRSQDSRSSARMTVPLTREVSEQLQSFARRHRLTVNAVVQGVWALMLA
                     GRSGRQDVVFGATTSGRPTDLPGAEDMIGLFINSLPVRVRLDPRRGVVEWLRALQEDQL
                     QARRYDYLPLTHIQALSELDPDQRLFDSLVVFENYPLDTEAAADQGLAVSDISAVEATN
                     YALNLVAYDGDRIDLVLAYDPDVFDEDTVRALRDDLLLQATAVAHADPEHTLGNLTLVG
                     PDQHRRLLDDWGRGAPVDAAKRHETLGGIFRAQLARTPDLPAAVAGDDRVTYAELHGAA
                     ARLAHHLIATGIRPGDRVGVALERGIPWLTAMLAVVHSGAVYVPLDPRYPTDRLEHMIA
                     DSGVTVILTETRFQDTLPADHPARTEAVDTLGAALAALPTTLPDLPEDPRRPAYIIYTS
                     GSTGLPKGVLVPHTGLSGLLTVPAENFRVRPGDRVLQFASPSFDASVLEVLTALGLGGT
                     LVLTPPGTLSAEELTDLLERHEVNHALILPAMLATLPRRELTHLRTLLVGAEPVQADLV
                     ARWAHGRRMVNGYGPTEATIGATFSGALSPERAGDPPIGRPYAGVRTYVLDGWLRPVAP
                     GTVGELYIGGEGVALGYLNRPAETAVRFLADPFGEPGSRMYRSGDLVRWTADGQLLFVG
                     RADTQIKVRGHRVELGEVETALAQHPAVGHAAVLLRHD"
     aSDomain        10..798
                     /aSDomain="Condensation"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_AQI70_RS36780_Condensation_DCL.1
                     "
                     /domain_subtypes="Condensation_DCL"
                     /evalue="2.10E-79"
                     /label="AQI70_RS36780_Condensation_DCL.1"
                     /locus_tag="AQI70_RS36780"
                     /protein_end="264"
                     /protein_start="1"
                     /score="258.7"
                     /specificity="C activity: active"
                     /specificity="Active site motif: HHVLLDG"
                     /tool="antismash"
                     /translation="VEAFAGAWQRVVDAVQALRVGVVWEGVAEPVRVVHRSVTLPVEVV
                     DWRGAGAEDVGARWDALVVADRERGLDLRQVPLARLTLARVGDERVRVLFTFHHVLLDG
                     WSLARVLSLVLDHHTARQRGTQPPAIVDRGLADHVRWLADRDLPAAEKYWRSVLSGYDT
                     PVDLPADRPLGRSQDSRSSARMTVPLTREVSEQLQSFARRHRLTVNAVVQGVWALMLAG
                     RSGRQDVVFGATTSGRPTDLPGAEDMIGLFINSLPVRVRLD"
     aSModule        10..2469
                     /domains="nrpspksdomains_AQI70_RS36780_Condensation_DCL.1"
                     /domains="nrpspksdomains_AQI70_RS36780_AMP-binding.1"
                     /incomplete
                     /locus_tags="AQI70_RS36780"
                     /tool="antismash"
                     /type="nrps"
     CDS_motif       298..354
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_AQI70_RS36780_0001"
                     /evalue="7.40E-05"
                     /label="C3_DCL_135-156"
                     /locus_tag="AQI70_RS36780"
                     /protein_end="116"
                     /protein_start="97"
                     /score="15.4"
                     /tool="antismash"
                     /translation="FHHVLLDGWSLARVLSLVL"
     CDS_motif       691..786
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_AQI70_RS36780_0002"
                     /evalue="1.40E-19"
                     /label="C5_DCL_263-294"
                     /locus_tag="AQI70_RS36780"
                     /protein_end="260"
                     /protein_start="228"
                     /score="62.5"
                     /tool="antismash"
                     /translation="DVVFGATTSGRPTDLPGAEDMIGLFINSLPVR"
     CDS_motif       835..957
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_AQI70_RS36780_0003"
                     /evalue="1.40E-19"
                     /label="C67_DCL_14fromHMM"
                     /locus_tag="AQI70_RS36780"
                     /protein_end="317"
                     /protein_start="276"
                     /score="62.6"
                     /tool="antismash"
                     /translation="QEDQLQARRYDYLPLTHIQALSELDPDQRLFDSLVVFENYP"
     aSDomain        1291..2469
                     /aSDomain="AMP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_AQI70_RS36780_AMP-binding.1"
                     /evalue="4.00E-105"
                     /label="AQI70_RS36780_AMP-binding.1"
                     /locus_tag="AQI70_RS36780"
                     /protein_end="821"
                     /protein_start="428"
                     /score="343.7"
                     /specificity="substrate consensus: X"
                     /tool="antismash"
                     /translation="FRAQLARTPDLPAAVAGDDRVTYAELHGAAARLAHHLIATGIRPG
                     DRVGVALERGIPWLTAMLAVVHSGAVYVPLDPRYPTDRLEHMIADSGVTVILTETRFQD
                     TLPADHPARTEAVDTLGAALAALPTTLPDLPEDPRRPAYIIYTSGSTGLPKGVLVPHTG
                     LSGLLTVPAENFRVRPGDRVLQFASPSFDASVLEVLTALGLGGTLVLTPPGTLSAEELT
                     DLLERHEVNHALILPAMLATLPRRELTHLRTLLVGAEPVQADLVARWAHGRRMVNGYGP
                     TEATIGATFSGALSPERAGDPPIGRPYAGVRTYVLDGWLRPVAPGTVGELYIGGEGVAL
                     GYLNRPAETAVRFLADPFGEPGSRMYRSGDLVRWTADGQLLFVGRADTQIKVR"
     CDS_motif       1477..1518
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_AQI70_RS36780_0004"
                     /evalue="6.60E-04"
                     /label="NRPS-A_a2"
                     /locus_tag="AQI70_RS36780"
                     /protein_end="504"
                     /protein_start="490"
                     /score="13.2"
                     /tool="antismash"
                     /translation="LAVVHSGAVYVPLD"
     CDS_motif       1714..1773
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_AQI70_RS36780_0005"
                     /evalue="7.10E-10"
                     /label="NRPS-A_a3"
                     /locus_tag="AQI70_RS36780"
                     /protein_end="589"
                     /protein_start="569"
                     /score="30.8"
                     /tool="antismash"
                     /translation="AYIIYTSGSTGLPKGVLVPH"
     CDS_motif       2263..2349
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_AQI70_RS36780_0006"
                     /evalue="6.20E-15"
                     /label="NRPS-A_a6"
                     /locus_tag="AQI70_RS36780"
                     /protein_end="781"
                     /protein_start="752"
                     /score="47.3"
                     /tool="antismash"
                     /translation="PGTVGELYIGGEGVALGYLNRPAETAVRF"
     CDS_motif       2434..2499
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_AQI70_RS36780_0007"
                     /evalue="8.80E-12"
                     /label="NRPS-A_a8"
                     /locus_tag="AQI70_RS36780"
                     /protein_end="831"
                     /protein_start="809"
                     /score="36.9"
                     /tool="antismash"
                     /translation="FVGRADTQIKVRGHRVELGEVE"
     assembly_gap    2553..3269
                     /estimated_length=717
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3270..>3838
                     /locus_tag="AQI70_RS36785"
                     /old_locus_tag="AQI70_36785"
     CDS             3272..>3838
                     /GO_function="GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /NRPS_PKS="Domain: Epimerization (39-189). E-value:
                     6.1e-62. Score: 201.3. Matches aSDomain:
                     nrpspksdomains_AQI70_RS36785_Epimerization.1"
                     /codon_start=2
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Condensation"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1127: condensation domain-containing protein"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /locus_tag="AQI70_RS36785"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AQI70_36785"
                     /product="condensation domain-containing protein"
                     /protein_id="WP_244182000.1"
                     /sec_met_domain="Condensation (E-value: 3.4e-38, bitscore:
                     118.4, seeds: 42, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MAGLAVSPKDVFDCQTVAVLAERVENAPQEDTADKRPAVTGPVRL
                     TPIQEWFFRSHTAAPHHFGMSMAATLPDDVDHTALATAMTALVEHHDALRLRFERTEDG
                     TWVQSVGALTDTEPLRHHDLSGLPQEQRTAELDHTVRTAQSGMDLATGPLFRALTFDLG
                     TDGTRLVMVAHHLVVDGVSWRVLLED"
     aSDomain        3390..>3838
                     /aSDomain="Epimerization"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_AQI70_RS36785_Epimerization.1"
                     /evalue="6.10E-62"
                     /label="AQI70_RS36785_Epimerization.1"
                     /locus_tag="AQI70_RS36785"
                     /protein_end="189"
                     /protein_start="39"
                     /score="201.3"
                     /specificity="E activity: active"
                     /specificity="Active site motif: HHLVVDG"
                     /tool="antismash"
                     /translation="TGPVRLTPIQEWFFRSHTAAPHHFGMSMAATLPDDVDHTALATAM
                     TALVEHHDALRLRFERTEDGTWVQSVGALTDTEPLRHHDLSGLPQEQRTAELDHTVRTA
                     QSGMDLATGPLFRALTFDLGTDGTRLVMVAHHLVVDGVSWRVLLED"
     CDS_motif       3405..3434
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_AQI70_RS36785_0001"
                     /evalue="2.50E-04"
                     /label="NRPS-E1"
                     /locus_tag="AQI70_RS36785"
                     /protein_end="54"
                     /protein_start="44"
                     /score="13.9"
                     /tool="antismash"
                     /translation="LTPIQEWFFR"
     CDS_motif       3465..3566
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_AQI70_RS36785_0002"
                     /evalue="2.60E-08"
                     /label="C2_LCL_024-062"
                     /locus_tag="AQI70_RS36785"
                     /protein_end="98"
                     /protein_start="64"
                     /score="26.3"
                     /tool="antismash"
                     /translation="MSMAATLPDDVDHTALATAMTALVEHHDALRLRF"
     CDS_motif       3777..>3838
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_AQI70_RS36785_0003"
                     /evalue="2.70E-12"
                     /label="NRPS-E2"
                     /locus_tag="AQI70_RS36785"
                     /protein_end="189"
                     /protein_start="168"
                     /score="38.7"
                     /tool="antismash"
                     /translation="LVMVAHHLVVDGVSWRVLLED"
ORIGIN
        1 tggatgtgga ggcgttcgcg ggggcctggc agcgggtggt tgacgcggtg caggcgttgc
       61 gggtcggggt ggtgtgggag ggcgttgcgg agccggtgcg ggtggtgcat cggtcggtga
      121 cgttgccggt ggaggtggtg gactggcgtg gggccggggc cgaggatgtg ggtgcccggt
      181 gggatgcgct ggtggtggcg gaccgggagc gggggctgga tctgcggcag gtgccgttgg
      241 cgcggctgac gctggcccgg gtgggggatg agcgggttcg ggtgttgttc acgttccacc
      301 atgtgctgtt ggacgggtgg agcctggccc gggtgctgtc cctcgtcctc gaccaccaca
      361 ccgcacgtca gcgcggcaca cagccccccg ccatcgtcga ccgtggcctc gccgaccacg
      421 tcaggtggct ggccgaccgc gacctgccgg cggccgagaa gtactggcgc tccgtcctct
      481 ccggctacga cacccccgtc gacctgccgg ccgaccgccc cctcggacgc tcccaggact
      541 cccgctcctc ggcacggatg accgtgcccc tgacccggga ggtctccgag cagctccagt
      601 cgttcgcgcg gcggcaccgg ctgacggtga acgcggtcgt gcagggcgtg tgggcgctga
      661 tgctggccgg acgcagcgga cggcaggacg tcgtgttcgg cgccaccacc tccgggcggc
      721 ccaccgacct gccgggcgcc gaggacatga tcggcctgtt catcaactcc ctaccggtac
      781 gcgtccgcct cgacccccgc cgcggcgtgg tggagtggct gcgggccctg caggaggacc
      841 agctccaggc ccgccgctac gactacctgc ccctgaccca catccaggca ctgagcgaac
      901 tcgaccccga ccaacgcctc ttcgacagcc tcgtcgtctt cgagaactac cccctcgaca
      961 ccgaggcggc agccgaccag ggcctggccg tcagcgacat cagcgcggtg gaggccacca
     1021 actacgccct caacctggtg gcctacgacg gcgaccggat cgacctcgtc ctcgcctacg
     1081 accccgacgt cttcgacgag gacaccgtgc gggcgctgcg cgacgacctg ctcctgcagg
     1141 ccaccgccgt cgcccacgcc gaccccgagc acaccctcgg caacctcacc ctggtcggcc
     1201 ccgaccagca ccgccgcctc ctcgacgact ggggccgcgg cgcccccgtg gacgcggcaa
     1261 agcggcacga gaccctcggc ggcatcttcc gggcccagct cgcccgcacc ccggacctgc
     1321 ccgccgcggt tgccggcgac gaccgagtca cctacgccga actgcacggc gccgccgccc
     1381 gcctggccca ccacctcatc gccaccggca tacgccccgg cgaccgggtc ggcgtcgccc
     1441 tggagcgggg catcccgtgg ctgaccgcga tgctggcggt cgtgcactcc ggcgccgtct
     1501 acgtgccgct cgacccccgg taccccaccg accggctgga gcacatgatc gccgactccg
     1561 gtgtgacggt gatcctgacc gagacccgct tccaggacac gctcccggcg gaccacccgg
     1621 cccggaccga ggcggtggac accttgggcg ccgccctggc ggccctgccc acgaccctgc
     1681 ccgacctccc cgaggacccc cgccggcccg cctacatcat ctacacctcc ggctccaccg
     1741 gactgcccaa gggcgtcctg gtgccgcaca ccggcctcag cggactgctc accgtccctg
     1801 cggagaactt ccgcgtccgg ccgggcgacc gggtcctgca gttcgcctca cccagcttcg
     1861 acgcctccgt cctggaggtg ctgaccgcgc tcggcctggg cggcacgctc gtcctgacgc
     1921 cccccggcac cctctccgcc gaggaactga ccgacctgct ggagcgccac gaggtcaacc
     1981 acgccctcat cctgcccgcc atgctggcca ccctgccccg ccgggaactt acgcacctgc
     2041 gcaccctgct ggtcggcgct gaacccgtcc aggccgacct cgtcgcccgc tgggcgcacg
     2101 gccggcgcat ggtcaacggc tacggcccca ccgaggccac catcggcgcg accttcagcg
     2161 gcgccctgtc gcccgagcgg gccggcgacc cgcccatcgg ccgcccctac gccggagtgc
     2221 gcacctacgt cctcgacggc tggctgcgcc ccgtggcccc cggcaccgtc ggcgagctct
     2281 acatcggcgg cgagggcgtc gcactcggct acctcaaccg ccccgccgag accgcggtcc
     2341 gcttcctggc cgacccgttc ggcgagccgg gcagccgtat gtaccgctcg ggcgacctgg
     2401 tgcgctggac cgccgacgga cagctgctct tcgtcggacg cgccgacacc cagatcaagg
     2461 tccgcggcca ccgcgtggaa ctgggcgagg tggagaccgc gctcgcccag caccccgccg
     2521 tcggacacgc cgccgtgctg ctccgccacg acnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc gccgccggac tggccgtctc gcccaaggac
     3301 gtcttcgact gccagacggt ggcggtcctc gccgagcgcg tggagaacgc cccccaggag
     3361 gacaccgccg acaagcggcc ggccgtcacc ggccccgtcc ggctgacccc catccaggag
     3421 tggttcttcc gcagccacac cgcggcaccc caccacttcg gcatgtcgat ggccgcgacc
     3481 ctgcccgacg acgtcgacca cacggccctg gccaccgcca tgaccgcgct ggtggagcac
     3541 catgacgccc tgcgcctgcg cttcgagcgc accgaggacg gcacctgggt ccagtcggtc
     3601 ggcgccctca cggacaccga accgctgcgc caccacgacc tctccggcct gccgcaggag
     3661 cagcgcacag ccgagctgga ccacacggtc cgcaccgccc agtccggcat ggacctggcc
     3721 accggcccgc tcttccgcgc cctgaccttc gacctcggca ccgacggcac ccgtctggtg
     3781 atggtggccc atcacttggt ggtggacggg gtgtcgtggc gggtgttgct ggaggact
//
