LOCUS       NZ_KQ947994            10498 bp    DNA     linear   CON 21-APR-2024
DEFINITION  Streptomyces curacoi strain DSM 40107 PRJNA299217_s011, whole genome
            shotgun sequence.
ACCESSION   NZ_KQ947994
VERSION     NZ_KQ947994.1
KEYWORDS    WGS; RefSeq.
SOURCE      Streptomyces curacoi
  ORGANISM  Streptomyces curacoi
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 256951)
  AUTHORS   Ruckert,C., Winkler,A., Kalinowski,J., Kampfer,P. and Glaeser,S.
  TITLE     Draft genome sequence of Streptomyces curacoi DSM 40107, type strain
            for the species Streptomyces curacoi
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 256951)
  AUTHORS   Ruckert,C., Winkler,A., Kalinowski,J., Kampfer,P. and Glaeser,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-OCT-2015) CeBiTec, Bielefeld University, Sequenz 1,
            Bielefeld 33615, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 13-OCT-2014
            Assembly Method                   :: Newbler v. 2.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 65.7x
            Sequencing Technology             :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_001513975.1-RS_2024_04_21
            Annotation Date                   :: 04/21/2024 02:05:31
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,531
            CDSs (total)                      :: 7,455
            Genes (coding)                    :: 7,251
            CDSs (with protein)               :: 7,251
            Genes (RNA)                       :: 76
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 204
            CDSs (without protein)            :: 204
            Pseudo Genes (ambiguous residues) :: 0 of 204
            Pseudo Genes (frameshifted)       :: 91 of 204
            Pseudo Genes (incomplete)         :: 154 of 204
            Pseudo Genes (internal stop)      :: 21 of 204
            Pseudo Genes (multiple problems)  :: 54 of 204
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 08:06:44
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 193997
            Orig. end                         :: 204495
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            KQ947994.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10498
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="melanin"
                     /region_number="2"
                     /rules="melC"
                     /tool="antismash"
     cand_cluster    1..10498
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="melC"
                     /kind="single"
                     /product="melanin"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10498
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5498](+)"
                     /cutoff="20000"
                     /detection_rule="melC"
                     /neighbourhood="5000"
                     /product="melanin"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5498
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="melC"
                     /neighbourhood="5000"
                     /product="melanin"
                     /protocluster_number="1"
     gene            705..1022
                     /locus_tag="AQI70_RS32305"
                     /old_locus_tag="AQI70_32300"
     CDS             705..1022
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016435074.1"
                     /locus_tag="AQI70_RS32305"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AQI70_32300"
                     /product="hypothetical protein"
                     /protein_id="WP_004001124.1"
                     /transl_table=11
                     /translation="MKNLKAAAVVVGSLAVAGTAGPAAAVDLSAPGLVDNGKTVARSLP
                     AAAKLPTGYVGNNVKRTADGVKQGVRNSSVVKTPVFGGTLPNPIDGQLLGGLPVGKPAI
                     K"
     gene            complement(1408..1728)
                     /locus_tag="AQI70_RS32310"
                     /old_locus_tag="AQI70_32305"
     CDS             complement(1408..1728)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004001125.1"
                     /locus_tag="AQI70_RS32310"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AQI70_32305"
                     /product="hypothetical protein"
                     /protein_id="WP_062155997.1"
                     /transl_table=11
                     /translation="MRNKVTAISAIAAGIVLATGGTATADSDTTTVKGGSGFSASPVVV
                     NEPQQGVAVVNGTLIDARCLAPWSNGAVLGGVAAPNSHYAACNTARINQAQNAPYVGGL
                     LF"
     misc_feature    1718..1735
                     /note="TFBS match to ArcA, Repressor of carbon oxidation,
                     confidence: weak, score: 17.18"
                     /tool="antismash"
     gene            complement(1787..2107)
                     /locus_tag="AQI70_RS32315"
                     /old_locus_tag="AQI70_32310"
     CDS             complement(1787..2107)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004001125.1"
                     /locus_tag="AQI70_RS32315"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AQI70_32310"
                     /product="hypothetical protein"
                     /protein_id="WP_062155998.1"
                     /transl_table=11
                     /translation="MRNKVTAISAVAAGIVLATGGTATADSDTTTVKGGSGFSASPVVV
                     NAPQQGIAVVNGSVVDLRCAVPWSNGAVLGGVVAPNSHYAACNTAPIDQSQNAAYRGGL
                     LF"
     gene            complement(2168..2503)
                     /locus_tag="AQI70_RS32320"
                     /old_locus_tag="AQI70_32315"
     CDS             complement(2168..2503)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007499734.1"
                     /locus_tag="AQI70_RS32320"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AQI70_32315"
                     /product="hypothetical protein"
                     /protein_id="WP_004001127.1"
                     /transl_table=11
                     /translation="MPKKFAVITALALSTVVAGAGVATAGEYDGNGKNTTVKGGSGFSS
                     SPVVVNAPQQGVLIVNGTLVDGRCLAPWSNGAVLGGVVPPNSHYAACNTAKVDQTQNAP
                     YAGGLLF"
     misc_feature    2554..2567
                     /note="TFBS match to ArgR, Regulator of arginine
                     biosynthesis genes, confidence: weak, score: 16.42"
                     /tool="antismash"
     gene            complement(2963..3352)
                     /locus_tag="AQI70_RS32325"
                     /old_locus_tag="AQI70_32320"
     CDS             complement(2963..3352)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007499736.1"
                     /locus_tag="AQI70_RS32325"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AQI70_32320"
                     /product="hypothetical protein"
                     /protein_id="WP_244181921.1"
                     /transl_table=11
                     /translation="MKKAVFTTAALAIGAGLVGGSAAAAAAAGGSGISTSPLGLMSPTK
                     VTPAVVPAADVVTRLARDGGIAKGSAFDKKKNSVGMSGVYLDGMDPEAAKRPTIPVKNP
                     AGSGSVQVRPLQAPTASGLGTLLPK"
     misc_feature    3544..3559
                     /note="TFBS match to LexA_variant_1, Repressor of DNA
                     damage response, confidence: weak, score: 20.04"
                     /tool="antismash"
     gene            3601..4311
                     /locus_tag="AQI70_RS32330"
                     /old_locus_tag="AQI70_32325"
     CDS             3601..4311
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004001129.1"
                     /locus_tag="AQI70_RS32330"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AQI70_32325"
                     /product="beta-glucanase"
                     /protein_id="WP_062156000.1"
                     /transl_table=11
                     /translation="MTIDTATFTPTWSEEFDRPIAWGAKWVGDRSSAYRYGDHNPDDNK
                     LDWLDPGCVTVRDGVATFTAAPAGRTLENGLQAWHTGLLTTEYSDEGFQVRTGDQIETR
                     VRLPHGAGAWPALWTWKDGENEVDSFEYHPDNPHLLELSNQVNGASTYHTDPDAVAPDR
                     WVTIGTRYGARSVEWYVNGESVFCDGAGVGADWSAYLILNLSVCAGRYHPAPCGPAPLT
                     FAVDYLRVYREAAR"
     gene            4308..4910
                     /locus_tag="AQI70_RS32335"
                     /old_locus_tag="AQI70_32330"
     CDS             4308..4910
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004001130.1"
                     /locus_tag="AQI70_RS32335"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AQI70_32330"
                     /product="vitamin K epoxide reductase family protein"
                     /protein_id="WP_062156001.1"
                     /transl_table=11
                     /translation="MTSAPGPRRTVGGGRAFAVLLVLTGAAGLLASWVITLDKFKLLQD
                     PDFTPGCSLNPVLTCGSVMKSEQASAFGFPNPMLGLVAYGIVICVGASLLAGAAFPRWY
                     WLAFEAGCLFGIGFVSWLQFESLYRINALCLWCCLAWLATILLFWSLTSFLVRHGLLPA
                     PGPVRNFLAEFGWVPPLLHIGIVGMLILTRWWDFWTS"
     gene            5001..5498
                     /locus_tag="AQI70_RS32340"
                     /old_locus_tag="AQI70_32335"
     CDS             5001..5498
                     /GO_function="GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /GO_process="GO:0042438 - melanin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     melanin: melC"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014672801.1"
                     /locus_tag="AQI70_RS32340"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AQI70_32335"
                     /product="tyrosinase family oxidase copper chaperone"
                     /protein_id="WP_062156002.1"
                     /sec_met_domain="melC (E-value: 3.3e-46, bitscore: 150.8,
                     seeds: 38, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MAVSADGVSVGAVQGAGPRASGAPAGRTRREVARLLLGSAAVACT
                     PVLAASRPSRSADEQRDTGTAFDETYRGRRIQGFPLPAAGLSVLGLEWRVTVDGRPLHL
                     MRRADGTWLSMIDHYSWYRTPLEATRAAVDELGPSRRLRDLAPGPFGGGHAHREAAHGV
                     RA"
     gene            5482..6342
                     /locus_tag="AQI70_RS32345"
                     /old_locus_tag="AQI70_32340"
     CDS             5482..6342
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019325459.1"
                     /locus_tag="AQI70_RS32345"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AQI70_32340"
                     /product="tyrosinase family protein"
                     /protein_id="WP_062156003.1"
                     /transl_table=11
                     /translation="MVYVRKNVRKLTRSERRRFVEALLELKRRGEYDEFVRLHIAFFRG
                     DGDTGLRAAHMTPSFLPWHRRFVLDLESALQSVDDSVTVPYWDWTRDRSTTAVPWTDDL
                     LGGNGRPRDRQVMTGPFAYVTGNWTITENITNVEYLTRDLGRSRNPIDLPTRADLNWAL
                     KDPVYDVAPWNSTVTKGFRNKMEGWGSDSNPWRNHNRVHRWVGGVMLGGASVNDPVFWL
                     VHSFMDMQWTRWQRRHRKHRYLPAKPPGRGSRHYGRIVARHEPLPPWDVTPDELEDMSE
                     IYRYA"
     gene            6404..6994
                     /locus_tag="AQI70_RS32350"
                     /old_locus_tag="AQI70_32345"
     CDS             6404..6994
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004001133.1"
                     /locus_tag="AQI70_RS32350"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AQI70_32345"
                     /product="DUF2087 domain-containing protein"
                     /protein_id="WP_062156004.1"
                     /transl_table=11
                     /translation="MATDPTEQLVRLFAEESRVRAFAAVALGAGSAEQVARAAGLSPKE
                     TATALRRLREQGVVTAADDGELGVAYGLFKQYARSAAQRENDSEAAESPASGDERTDMV
                     VRTFVRDGRLVRLPAQWARKKLVLRYIADRTFEPGVEYPERTVDDKLRAWCEDSDDIDH
                     VTLRRYLVDLHHLHRSEGVYRRPGGETESAGIA"
     misc_feature    complement(6420..6434)
                     /note="TFBS match to IolR, Regulator of the inositol
                     catabolism, confidence: medium, score: 19.94"
                     /tool="antismash"
     misc_feature    6421..6435
                     /note="TFBS match to IolR, Regulator of the inositol
                     catabolism, confidence: medium, score: 19.09"
                     /tool="antismash"
     gene            complement(7004..7963)
                     /locus_tag="AQI70_RS32355"
                     /old_locus_tag="AQI70_32350"
     CDS             complement(7004..7963)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) 2-Hacid_dh_C"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1072: dehydrogenase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014672797.1"
                     /locus_tag="AQI70_RS32355"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AQI70_32350"
                     /product="D-2-hydroxyacid dehydrogenase family protein"
                     /protein_id="WP_062156005.1"
                     /sec_met_domain="2-Hacid_dh_C (E-value: 1.5e-54, bitscore:
                     177.9, seeds: 135, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRLRCAVLDDFQNVATEVADWSPLADDVEVVPFTTHFPDEDTLAA
                     ALADFDIVVTLRERVPFPASLIARLPRLKLLIASGMRNSVIDYAAAEAHGVTVCGTASS
                     STPPVELTWALLLGLARGIVQENGALRSGGPWQSTVGADLHGRRLGLLGLGKIGSRVAQ
                     VGLAFGMHVSAWSQNLTKEYADEVGVHLAASKEELLAESDFVSVHLALSDRTRGLLGAP
                     ELALLKPAAYLINTSRAAIVDQDALLAALHEGRIAGAGIDVFDVEPLPTDHPMRTAPRL
                     LATPHLGYVSQANYATYYGQAVEDIQAYLAGTPVRRLP"
     gene            8236..9204
                     /locus_tag="AQI70_RS32360"
                     /old_locus_tag="AQI70_32355"
     CDS             8236..9204
                     /GO_function="GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /NRPS_PKS="Domain: NAD_binding_4 (6-176). E-value: 9e-11.
                     Score: 33.3. Matches aSDomain:
                     nrpspksdomains_AQI70_RS32360_NAD_binding_4.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) RmlD_sub_bind"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1010: NAD-dependent epimerase/dehydratase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004001135.1"
                     /locus_tag="AQI70_RS32360"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AQI70_32355"
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="WP_062156006.1"
                     /sec_met_domain="RmlD_sub_bind (E-value: 2.5e-08, bitscore:
                     27.2, seeds: 37, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSPRILITGASGFIGGHTAAAALGTLDGATPSSIRLMLRNPDAGA
                     GTWGSGPGAGSRAVDVVRGDLADPGSLRGICDGVDVVLHCASYIGGDERAAVTVNDHGT
                     RALVAEAARAGVGRVVYVSTAAVYGRGPFRAALPDRLPPAPASPTSRSRAAAERHVLDA
                     GGVVLRPHLVLGPGDRWVVPGLVTLLGLLSAGLRGGTARHSVVDVRALGRAVVAAGLSD
                     RDLAGAHHVNHPDPVGTSELLDTVADLLGLRLPGAPPTVDEARARLAGQPRGLHHLGML
                     AVDHWFADDGFWSGVGVGPGAGFAKSLAEHAAWYREFLGQR"
     aSDomain        8254..8763
                     /aSDomain="NAD_binding_4"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_AQI70_RS32360_NAD_binding_4.1"
                     /evalue="9.00E-11"
                     /label="AQI70_RS32360_NAD_binding_4.1"
                     /locus_tag="AQI70_RS32360"
                     /protein_end="176"
                     /protein_start="6"
                     /score="33.3"
                     /tool="antismash"
                     /translation="ITGASGFIGGHTAAAALGTLDGATPSSIRLMLRNPDAGAGTWGSG
                     PGAGSRAVDVVRGDLADPGSLRGICDGVDVVLHCASYIGGDERAAVTVNDHGTRALVAE
                     AARAGVGRVVYVSTAAVYGRGPFRAALPDRLPPAPASPTSRSRAAAERHVLDAGGVVLR
                     PHLVLGP"
     gene            9265..10053
                     /locus_tag="AQI70_RS32365"
                     /old_locus_tag="AQI70_32360"
     CDS             9265..10053
                     /NRPS_PKS="Domain: PKS_KR (10-180). E-value: 4.8e-12.
                     Score: 38.1. Matches aSDomain:
                     nrpspksdomains_AQI70_RS32365_PKS_KR.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) adh_short"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1001: short-chain dehydrogenase/reductase SDR"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006129466.1"
                     /locus_tag="AQI70_RS32365"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="AQI70_32360"
                     /product="SDR family oxidoreductase"
                     /protein_id="WP_062156007.1"
                     /sec_met_domain="adh_short (E-value: 2.5e-31, bitscore:
                     102.9, seeds: 230, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MDTQRALTGKVALVTGAGRGIGRAIAARLARDGALVAVHYGRSEA
                     AAHEVVAEIRKNGGRAFPVGAELGVPGDAAAVFEAFDAGLGERTDAAADAEGSLDILVN
                     NAGVSGSGPVGEAVPEVFDRMIAVNAKAPFFLVQSALPRMRDGGRIINISSLASRHAFP
                     ESLAYAMSKGALDTMTLCLAKELGARGITVNTVAPGFIETDMNARRRATPEARAALAAR
                     SVFDRIGRPSDVADVVAFLASDDARWVTGQYLDVSGGTDL"
     aSDomain        9295..9804
                     /aSDomain="PKS_KR"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_AQI70_RS32365_PKS_KR.1"
                     /evalue="4.80E-12"
                     /label="AQI70_RS32365_PKS_KR.1"
                     /locus_tag="AQI70_RS32365"
                     /protein_end="180"
                     /protein_start="10"
                     /score="38.1"
                     /specificity="KR activity: inactive"
                     /specificity="KR stereochemistry: C2"
                     /tool="antismash"
                     /translation="VALVTGAGRGIGRAIAARLARDGALVAVHYGRSEAAAHEVVAEIR
                     KNGGRAFPVGAELGVPGDAAAVFEAFDAGLGERTDAAADAEGSLDILVNNAGVSGSGPV
                     GEAVPEVFDRMIAVNAKAPFFLVQSALPRMRDGGRIINISSLASRHAFPESLAYAMSKG
                     ALDTMTL"
     CDS_motif       9301..9366
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_AQI70_RS32365_0001"
                     /evalue="9.90E-05"
                     /label="PKSI-KR_m1"
                     /locus_tag="AQI70_RS32365"
                     /protein_end="34"
                     /protein_start="12"
                     /score="15.0"
                     /tool="antismash"
                     /translation="LVTGAGRGIGRAIAARLARDGA"
ORIGIN
        1 gccgccacca ggtccgtcac gacccgcgcg acaccgccgt cgacgggctg ggcgacatgc
       61 aggatgtgcg gggagatgcg catgaccgat gctgcccttc cgaagaaatg agtcccaacc
      121 agcaacacga tgtgcggccc gcgttcccga tataagatcc cgcacctgtt cgagagggtc
      181 tgtggttcaa tcggcttcct ggattccgag ggtgagcagc accagccact tatccatact
      241 tatctgagaa gcggtcggcc aggtgggaac ggcagcgttt tccgagttgt ttcccgtgcg
      301 ggaccaccca tttggagccc cggggtggcc ggcgcgaaaa aggcctctca tcagggcgga
      361 cgggccgttc acccctgaca gttcgtcaca tggccggggg ccaccggttg cggacgcggc
      421 ggaccggttt aacggtgatc acacgattgt ccggctctct gatgcaggac cggcgcctga
      481 ggcgaagcag ccgccgatcc tccagacccg ttgtgccgat cacggtcggc acgacgggtc
      541 ttttcgtatg agttacctgg aacttgtcgg cagcagagtt tcccgagggg ccccctgtcg
      601 ttagcccagg agaactgaga ccgagtcact cgcgacgcct gagcgatgaa atcccctgcg
      661 gggagcgcga gttacggaag acaagtttcc aggaaaggaa cacgatgaag aacctgaagg
      721 ctgctgccgt tgtcgtcggc tccctcgccg ttgccggtac cgctgggccc gccgccgcgg
      781 tggacctgtc ggcgcccggc cttgtcgaca acgggaagac ggtcgcccgc agcctccccg
      841 cggccgccaa gctgccgacg ggctacgtgg gcaacaacgt gaagcgcacg gcggacggcg
      901 tgaagcaggg cgtgcggaac tcgagcgtgg tgaagacccc cgtcttcggc ggcaccctgc
      961 cgaaccccat cgacggccag ctgctcggtg gacttccggt cggcaagccg gcgatcaagt
     1021 aggcgatttc gttcctcggc tcgatgttcc gggccggctc cgagtccttc ggagccggcc
     1081 cttcggcatg tggggcgaag tgcaattgtc ctatcgcccc gttgggccga acggacgatt
     1141 cagggtgacc cctggcacaa cagcttcgtt ggaccaactg aatgggcgct cttcctcctt
     1201 gctggcgctc cctcatggac cggtgatccg cctgtcggcg tttttgccgg tcgcctcctg
     1261 accgtgggcc ctgtctgcgt tacgacgcgg acagggccca cgggtttggt ggggaaccca
     1321 ctgctccggc gggaactcgc cgagggcgcg aaaatgccga tggcggtcca ccggcttccg
     1381 gcggaccgcc atcgggcgaa ggactgctca gaacagcagg ccgcccacgt acggggcgtt
     1441 ctgcgcctgg ttgatcctcg ccgtgttgca ggcggcgtag tgcgagttcg gcgcggcgac
     1501 gccaccgagg acggctccgt tggaccacgg ggcaaggcag cgagcgtcga taagagtgcc
     1561 gttcactaca gcgacaccct gctgcggttc gttgacgacg accggggaag ccgagaatcc
     1621 gctaccgccc ttgacagtgg tggtgtcgct gtcggccgtg gcggtgccgc cggtcgcaag
     1681 gacgatgcct gccgcgatgg cggaaatggc cgtaaccttg ttacgcatga gttgagcctc
     1741 cgggctgcgt gtgggtttac ctgctccgaa ttgctcggag caggggtcag aacagcagac
     1801 cgcccctgta ggcggcgttc tgcgactggt cgatcggggc cgtgttgcag gcggcatagt
     1861 gcgagttcgg tgcgactacg ccgccgagga cggctccgtt cgaccacggc acggcacagc
     1921 ggagatcgac gacgctgccg ttgacgacgg caataccctg ctgaggcgcg ttgacgacga
     1981 ccggggaagc cgagaaacca ctaccgccct tgacggtggt ggtgtcgctg tcggccgtgg
     2041 cggtgccgcc ggtcgcaagg acgatgcctg ctgccacggc ggaaatggcc gtaaccttgt
     2101 tacgcatgat ttgagccttt ccgggctgcg tgtgggagta cctgctccga atcgctcggg
     2161 acaggggtca gaacagcagg ccgcccgcgt acggggcgtt ctgcgtctgg tccaccttgg
     2221 ccgtgttgca ggcggcgtaa tgcgagttcg gcgggacgac accaccgagt acggctccgt
     2281 tggaccacgg ggcaaggcaa cgaccgtcga cgagggtgcc gttgacgatg agaacgccct
     2341 gctggggcgc gttcacgacg acaggggacg acgagaagcc actacccccc ttgacagtgg
     2401 tgttcttgcc gttgccgtcg tattcaccag cggtggcaac gccggctccg gcgacgacag
     2461 tgctgagggc gagggccgtg atgaccgcga acttcttggg caactcggtt ccttcctgaa
     2521 ttcacggttg cgcacgctta cgaaaaggaa acccgtatat gcacgcataa gtcacggtgg
     2581 gaacattcaa acgtgggcag attccatcgg atggcgcaac caagcgggct cgcaggtgcc
     2641 tccggcttta cgttcactca tgcggaacgc cctgccgaag gcggacgcgg aggtggaatc
     2701 cgtgcggtct tccgggaaat ttaggggcgg ttgagccgaa tgagccggcc cggccggacg
     2761 cggacgtgcg tccggcccac cggcaaggtg cgggccggtg ggccggacag acggttcgcg
     2821 ggtcagtggg ctttcgggcc cgccatatgc ggaggtggag ctgtgggtcc ggatccagcg
     2881 cctgggcctg gctgcggttt ccagccgccg agaaccggat cagagtctca gaattccggg
     2941 cccagctctg tcccgccgat tgtcacttgg gaaggagggt gccgagcccc gaggcggtcg
     3001 gtgcctgcag cgggcggacc tgtacagagc cgcttccggc cgggttcttc accggaatcg
     3061 tgggccgctt ggcagcctcc gggtccattc cgtccaggta aacgccggac attcccaccg
     3121 agttcttctt cttgtcgaac gcgctgccct tcgcgatccc gccgtcacga gccagccgcg
     3181 tcacgacgtc ggccgcgggc acgaccgccg gggtcacctt ggtcggggac atgagcccga
     3241 gcgggctggt ggagattccc gagcccccgg cggccgctgc cgcggcggcg gagccaccca
     3301 caagacccgc accgatggcc aacgccgcag tggtgaaaac tgccttcttc atgattcacc
     3361 tcgctagaaa gtacctctga tccgacggtt ggagaacata gcagtccagc ggccgtgcaa
     3421 aagcgacgcg ttgctccata cgcgtgaaag tcgcaggggt gaagaatgga atgcacagag
     3481 gcattcggag ggatgggcat ctggtcggcg aatcgattga tcctatggtg cgtccggcct
     3541 aattcgagcg cctgcgcgac gtggaatggg aaaaggggca ggtggcctac cctcgccgcc
     3601 atgaccatcg acaccgccac gttcacgccg acctggtccg aggagttcga ccgcccgatc
     3661 gcctggggcg ccaaatgggt cggcgacaga tcgagcgcct accgctacgg cgatcacaac
     3721 cccgacgaca acaagctcga ctggctcgac cccggctgtg tgaccgtccg cgacggtgtg
     3781 gccaccttca ccgcggcacc ggccggccgc accctggaga acggtctgca ggcctggcac
     3841 accggcctgc tcaccaccga atactccgac gagggcttcc aggtgcgcac cggcgaccag
     3901 atcgagaccc gggtcagact gccccacggg gccggagcct ggccggcgct gtggacctgg
     3961 aaggacggcg agaacgaggt cgactccttc gagtaccacc cggacaaccc gcacctgctg
     4021 gagctgtcca accaggtcaa cggtgcgtcg acgtaccaca ccgaccccga cgcggtcgcc
     4081 ccggaccggt gggtgaccat cggcacccgc tacggcgcgc gttccgtcga gtggtacgtc
     4141 aacggcgaga gcgtcttctg cgacggcgcc ggtgtcggcg ccgactggtc ggcgtatctc
     4201 atcctgaacc tgtcggtgtg cgccggccgg taccacccgg ccccctgcgg cccggctccc
     4261 ctcaccttcg ccgtcgacta cctgcgcgtc taccgcgagg ccgcgcggtg acctccgctc
     4321 cgggcccgcg acggaccgtg ggcggcggcc gtgcgttcgc cgtgctgctg gtgctcaccg
     4381 gggcggccgg gctgctggcc tcctgggtca tcaccctcga caagttcaag ctgcttcagg
     4441 acccggactt cacccccggc tgcagcctga acccggtgct gacctgcggc agcgtgatga
     4501 agagcgaaca agcctcggcc ttcgggttcc ccaacccgat gctgggcctg gtcgcctacg
     4561 gcatcgtgat ctgcgtcggc gcgagcctgc tcgccggagc cgccttcccg cgctggtact
     4621 ggctggcctt cgaggccgga tgcctgttcg gcatcgggtt cgtctcctgg ctgcagttcg
     4681 agtccctgta ccggatcaac gccctgtgcc tgtggtgctg cctggcctgg ctcgcgacca
     4741 tcctgctgtt ctggtccctg acgtccttcc tcgtgcggca cggcctgctg cccgcaccgg
     4801 gcccggtgcg gaacttcctc gcggagttcg gctgggtgcc tccgctgctg cacatcggca
     4861 tcgtcgggat gctgatcctg acccgctggt gggacttctg gacgagctga ccattgcacc
     4921 gtatgggcgc ttcggtggtg accgtggagc gtccccgtcg gttactcgta cgaacagccg
     4981 aaccgtacga ggagtgagcg atggccgtca gtgcagacgg agtgtcggtg ggtgcggtgc
     5041 agggggcggg tccccgggcg agcggcgcgc cggcggggcg gacacggcgg gaggtggcgc
     5101 ggctgctgct cggctccgcc gccgtggcgt gcacgccggt gctcgccgcc tcccggccct
     5161 cgcggtccgc ggacgaacag cgggacaccg gcaccgcctt cgacgagacc taccgcggcc
     5221 gccggatcca gggcttcccg cttcccgccg cgggtctcag tgtgctcggg ctcgaatggc
     5281 gcgtcaccgt ggacggccgc ccgctgcacc tgatgcgccg ggccgacggc acctggctga
     5341 gcatgatcga ccactacagc tggtaccgga ccccgctgga ggcgacccgc gcggccgtcg
     5401 acgaactcgg gcccagccgg cggctgcgcg acctggcccc cgggccgttc ggcggcgggc
     5461 acgcgcacag ggaggcagca catggtgtac gtgcgtaaga acgtgcggaa gctgacgcgc
     5521 agtgagaggc ggcggttcgt cgaggcgctg ctcgaactca agcggcgcgg tgagtacgac
     5581 gagttcgtac ggctgcacat cgccttcttc cgcggggacg gtgacacggg tctgcgcgcg
     5641 gcccatatga cgccctcctt cctgccctgg caccgcaggt tcgtgctgga cctggagagc
     5701 gccctgcaga gtgtcgacga ctcggtgacg gtgccgtact gggactggac ccgggaccgt
     5761 tcgaccaccg ccgtgccgtg gaccgacgat ctgctcggcg gcaacgggcg gccgcgggac
     5821 cggcaggtga tgaccgggcc gttcgcctac gtgacgggca actggaccat cacggagaac
     5881 atcaccaacg tcgagtacct cacccgggac ctgggccgct cccgtaaccc gatcgacctg
     5941 cccacccggg ccgatctgaa ctgggcgctg aaggacccgg tgtacgacgt cgccccctgg
     6001 aactccacgg tcaccaaggg cttccgcaac aagatggagg ggtggggcag cgacagcaac
     6061 ccctggcgca accacaaccg ggtgcatcgc tgggtcgggg gcgtcatgct cggcggcgcg
     6121 tccgtcaacg atcccgtctt ctggctggtc cactccttca tggacatgca gtggacccgc
     6181 tggcagcggc gccaccgcaa acaccgctat ctgcccgcca agccgcccgg ccggggcagc
     6241 cgccactacg gccggatcgt cgcccggcac gagccgctgc cgccgtggga cgtgaccccg
     6301 gacgagctgg aggacatgtc cgagatctac cggtacgcct gagacccggc gcgcggaaaa
     6361 aaaatccctc agccccgcgg cgggcgtcca ctagcctccc ggcatggcaa ccgatccgac
     6421 ggaacaactg gtccggctgt tcgccgagga gagccgggtg cgcgccttcg ccgcggtcgc
     6481 gctgggcgcg ggcagtgccg agcaggtcgc ccgggcggcg gggctgtcgc cgaaggagac
     6541 cgcgacggcg ctgcggcggc tgcgggagca gggcgtggtg acggcggcgg acgacgggga
     6601 gctgggcgtc gcctacggcc tgttcaagca gtacgcgcga tcggcggcgc aacgggagaa
     6661 cgactccgag gccgccgaga gccccgcctc cggggacgag cggaccgaca tggtcgtacg
     6721 gaccttcgtc cgggacggcc ggctggtccg gctgcccgcg cagtgggccc gcaagaagct
     6781 ggtactgcgg tacatcgccg atcggacctt cgagccgggc gtcgagtacc cggagcggac
     6841 cgtcgacgac aagctgcgcg cctggtgcga ggacagcgac gacatagacc atgtgacact
     6901 ccggcgctac ctggtggacc tgcaccatct gcaccggagc gagggcgtct accgacggcc
     6961 gggcggggag accgagtccg ccggtatcgc ctgaccgccg ccgtcagggc agccggcgta
     7021 ccggtgtgcc cgccaggtag gcctggatgt cctcgacggc ctgaccgtag tacgtggcgt
     7081 agttggcctg ggagacatag cccaggtggg gtgtggcgag caggcggggg gcggtgcgca
     7141 tcgggtggtc ggtggggagg ggctcgacgt cgaagacgtc gatgccggcg ccggcgatgc
     7201 ggccctcgtg caaggcggcg agcagggcgt cctggtcgac gatcgccgcg cgtgaggtgt
     7261 tgatcagata cgccgccggc ttgaggaggg cgagttcggg cgcgccgagc aggccgcggg
     7321 tgcggtcgct gagggccagg tggacggaga cgaagtcgct ctcggcgagc agctcctcct
     7381 tggaggcggc caggtggacg ccgacctcgt cggcgtactc cttggtgagg ttctggctcc
     7441 aggcgctgac gtgcatgccg aaggcgaggc cgacctgggc cacccggctg ccgatcttgc
     7501 ccaggcccag cagcccgagg cggcggccgt gcagatcggc gcctaccgtg ctctgccaag
     7561 gaccgccgga gcgcagggcg ccgttctcct ggacgatgcc gcgggcgagg cccagcagca
     7621 gggcccaggt gagttcgacg ggcggggtcg aggagctggc cgtaccgcag acggtgacgc
     7681 cgtgcgcctc ggcggcggcg tagtcgatga ccgagttgcg catgccggag gcgatcagca
     7741 gcttcagccg gggcagacgg gcgatcaggg aggcggggaa ggggacgcgt tcgcgcaggg
     7801 tgacgacgat gtcgaagtcg gcgagggccg cggccagggt gtcctcgtcg gggaagtggg
     7861 tggtgaaggg gacgacctcc acgtcgtccg cgagcgggga ccagtcggcg acctcggtcg
     7921 ccacgttctg gaagtcgtcg agcacagcac agcgcagccg cacggtgggg gtcctttcgt
     7981 ggggtgtcag gggcattgtc gccaacgcgc cgggccggac gggatgccgg gcggcgagat
     8041 gcggggcgag gtggtcgcgg aggcgttcac cgcgtactcg cggaagacgt gctgtcctgc
     8101 ctggtcagag agcttcctct gccgggtgcg cgcaccctca cgtcgcgtgt tgagaaccct
     8161 tgtggatcgc acgtaactta ctcgcgggcg gcgtcgcctc gctgcggacc gccggccgag
     8221 agggagggac ccgccttgtc accgcgcatc ctcatcaccg gggccagtgg tttcatcggg
     8281 ggccatacgg ccgccgcggc cctcgggacc ctcgatggcg ccacgccgtc ctcgatccgc
     8341 ctcatgctgc gcaaccccga tgcgggggcg gggacttggg gcagcggtcc cggcgccggc
     8401 tcccgcgcgg tggacgtcgt gcggggcgac ctggccgatc ccgggtccct gcgcgggatc
     8461 tgcgacggcg tcgacgtcgt cctgcactgt gcctcgtaca tcggcggcga cgaacgcgcc
     8521 gccgtcaccg tcaacgatca cgggacgcgt gccctcgtgg cggaggcggc gcgcgcgggt
     8581 gtgggacgcg tggtctatgt gagcacggcc gcggtctacg ggagagggcc gttccgcgcc
     8641 gcgctcccgg accggttgcc gcccgctccg gcgtcaccga ccagccgctc ccgcgccgcc
     8701 gccgagcggc atgtgctgga cgcgggcggg gtcgtcctgc gcccgcacct ggtgctcggg
     8761 ccgggggacc ggtgggtggt gcccggcctg gtcaccctgc tggggctgct ctcggcgggt
     8821 ctgaggggag gcaccgcccg gcactccgtc gtcgacgtcc gggcgttggg gcgcgcggtg
     8881 gtcgccgcgg ggctctcgga ccgggacctc gcgggcgcgc atcacgtcaa ccacccggat
     8941 ccggtgggca cttcggagct gctcgacacc gtggccgacc tgctcggcct gcgtctgccg
     9001 ggtgcgccgc cgaccgtcga cgaggcccgc gcacggctcg ccgggcagcc gcgggggctg
     9061 catcacctcg gcatgctcgc cgtggaccac tggttcgcgg acgacggctt ctggagcggt
     9121 gtcggggtcg gtcccggggc ggggttcgca aagtcgctgg ctgaacacgc cgcgtggtac
     9181 cgggagttcc tcgggcagcg gtgacgaggt gatgcggtga cgaggtgatg cggtgacgcg
     9241 gtggcgaggt gatacagcag tgtcatggat acccaacgcg cattgacggg caaggtcgca
     9301 ctggtcaccg gggccgggcg gggcatcggc agagccatcg cggcgcggct cgcccgggac
     9361 ggggctctgg tcgccgtcca ctacggccgc agcgaggccg ccgcccatga ggtggtggcg
     9421 gagatacgga agaacggtgg gcgggccttc cccgtgggag cggagctcgg ggtgcccggc
     9481 gacgccgccg cggtcttcga ggccttcgac gccggtctgg gcgagcggac ggacgcggcg
     9541 gcggacgccg agggctcgct cgacatcctg gtgaacaacg cgggggtgag cggctcgggg
     9601 ccggtggggg aggcggttcc cgaggtcttc gaccggatga tcgccgtcaa cgccaaggcc
     9661 ccgttcttcc tggtgcagag cgcgctgccg cggatgcggg acggcgggcg catcatcaac
     9721 atctcctcgc tcgcctcacg gcacgcgttc cccgagtccc tcgcgtacgc catgtccaaa
     9781 ggggcgctgg acacgatgac gctgtgcctg gcgaaggaac tcggcgcccg tggcatcacc
     9841 gtcaacacgg tcgcgcccgg cttcatcgag acggacatga acgcccggcg cagggcgacc
     9901 cccgaggcgc gcgccgcgct ggccgcgcgc tccgtcttcg accgcatcgg caggccttcc
     9961 gacgtggccg acgtggtggc gttcctcgcc tccgacgacg cccgctgggt caccggccag
    10021 tacctcgacg tcagcggggg cacggacctg tgacctgacc ggcgtttctc ctgccccggc
    10081 cggtgcgcgg tggtcggacc agcgtggccg ggcggatgtg tgtggccggc tcacctgttg
    10141 tcggcggacc ccccgaccgc accgtgaaag caccccctcc gctctggaga acagcggggg
    10201 tgccgagtta gggggtcctg gagatgactg ccgtgaacgc cgtacgacgc cttcaaccgg
    10261 aggtgccgga cgccaccgtc gccgagctgt gcgccgaact gttcgccctg ctgccgcgtg
    10321 cggaccagcg ggagaagggg gcgcagtatc tgtacggcct gctgacggcc gacggacgca
    10381 agaccgtgcg caacatcgcc gcccatgtcg ggggcggttc ggccgagcag agcctgcatc
    10441 acttcgtgtc cgcgtccacc tgggactgga tgcccgtgcg ggccgcgctg tgccggta
//
