LOCUS       NZ_LNBE01000011         8058 bp    DNA     linear   CON 29-MAR-2024
DEFINITION  Streptomyces roseifaciens strain MBT76 scaffold_11, whole genome
            shotgun sequence.
ACCESSION   NZ_LNBE01000011
VERSION     NZ_LNBE01000011.1
KEYWORDS    WGS; RefSeq.
SOURCE      Streptomyces roseifaciens
  ORGANISM  Streptomyces roseifaciens
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 8058)
  AUTHORS   Wu,C., Zhu,H., van Wezel,G.P. and Choi,Y.H.
  TITLE     Metabolomics-guided analysis of isocoumarin production by
            Streptomyces species MBT76 and biotransformation of flavonoids and
            phenylpropanoids
  JOURNAL   Metabolomics 12, 90 (2016)
   PUBMED   27073352
REFERENCE   2  (bases 1 to 8058)
  AUTHORS   Wu,C., Du,C., Choi,Y.H. and Van Wezel,G.P.
  TITLE     The complementarity of genome-mining and NMR metabolic-profiling
            facilitates lead-finding
  JOURNAL   Unpublished
REFERENCE   3  (bases 1 to 8058)
  AUTHORS   Wu,C., Du,C., Choi,Y.H. and Van Wezel,G.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2015) Molecular Biotechnology, Institute of
            Biology Leiden, Sylviusweg 72, Leiden 2333BE, Netherlands
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Velvet v. 1.2.06
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 200.0x
            Sequencing Technology             :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_001445655.1-RS_2024_03_28
            Annotation Date                   :: 03/28/2024 03:28:28
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,528
            CDSs (total)                      :: 7,450
            Genes (coding)                    :: 7,245
            CDSs (with protein)               :: 7,245
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 205
            CDSs (without protein)            :: 205
            Pseudo Genes (ambiguous residues) :: 0 of 205
            Pseudo Genes (frameshifted)       :: 77 of 205
            Pseudo Genes (incomplete)         :: 168 of 205
            Pseudo Genes (internal stop)      :: 20 of 205
            Pseudo Genes (multiple problems)  :: 56 of 205
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 13:37:43
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 0
            Orig. end                         :: 8058
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            LNBE01000011.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     gene            complement(<1..4857)
                     /locus_tag="AS857_RS36680"
     CDS             complement(<1..4857)
                     /NRPS_PKS="Domain: PKS_Docking_Nterm (1-16). E-value:
                     0.0013. Score: 10.2. Matches aSDomain:
                     nrpspksdomains_AS857_RS36680_PKS_Docking_Nterm.1"
                     /NRPS_PKS="Domain: PKS_KS(Modular-KS) (20-446). E-value:
                     2.8e-181. Score: 595.0. Matches aSDomain:
                     nrpspksdomains_AS857_RS36680_PKS_KS.1"
                     /NRPS_PKS="Domain: PKS_AT (553-831). E-value: 2.8e-108.
                     Score: 353.7. Matches aSDomain:
                     nrpspksdomains_AS857_RS36680_PKS_AT.1"
                     /NRPS_PKS="Domain: PKS_KR (1165-1340). E-value: 2.3e-58.
                     Score: 189.1. Matches aSDomain:
                     nrpspksdomains_AS857_RS36680_PKS_KR.1"
                     /NRPS_PKS="Domain: ACP (1445-1517). E-value: 9.7e-32.
                     Score: 101.1. Matches aSDomain:
                     nrpspksdomains_AS857_RS36680_ACP.1"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters) T1PKS:
                     PKS_AT"
                     /gene_functions="biosynthetic (rule-based-clusters) T1PKS:
                     mod_KS"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) ketoacyl-synt"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) adh_short"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Polysacc_synt_2"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PP-binding"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1021: malonyl CoA-acyl carrier protein transacylase"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981853.1"
                     /locus_tag="AS857_RS36680"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="type I polyketide synthase"
                     /protein_id="WP_275477398.1"
                     /sec_met_domain="mod_KS (E-value: 9.8e-227, bitscore:
                     739.8, seeds: 217, tool: rule-based-clusters)"
                     /sec_met_domain="ketoacyl-synt (E-value: 1.7e-95, bitscore:
                     306.4, seeds: 77, tool: rule-based-clusters)"
                     /sec_met_domain="PKS_AT (E-value: 9.2e-110, bitscore:
                     353.7, seeds: 1682, tool: rule-based-clusters)"
                     /sec_met_domain="adh_short (E-value: 7.4e-61, bitscore:
                     192.3, seeds: 230, tool: rule-based-clusters)"
                     /sec_met_domain="Polysacc_synt_2 (E-value: 2e-09, bitscore:
                     24.0, seeds: 77, tool: rule-based-clusters)"
                     /sec_met_domain="PP-binding (E-value: 5.9e-16, bitscore:
                     46.0, seeds: 164, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MDLRHTRRQLGELEAKDTEPIAIVGMACRYPGGVASPEDLWHLVS
                     SGADAVTGFPADRGWDLDAIHDPEPGRTGRTYVREGGFLHDAADFDPAFFGISPREALV
                     MDPQQRLLLQTSWEAFERAGIDPATARGTRAGVFVGTNGQDHAYLQRPGDSDIEAHLVT
                     ANTAAVVSGRISYALGLEGPAVTVDTACSSSLVALHLAAQALRRGECSLALAGGATVMA
                     TPNTFIAFSAQRGLSPDGRCRAFSSTADGTGMSEGVGVLLLERLSDARRLGHDVLAVIR
                     GSAVNQDGASNGLTAPNGPSQVRVIQQALENARLTASQVDAVEAHGTATTLGDPIEAEA
                     LLATYGQEHPGDRPLWLGSVKSNLGHTQAASGVAGVIKMVMAIRNGVLPQTLHVDEPTS
                     QVDWSAGGVRLLTEAVQWPESDHPRRAAVSSFGVSGTNAHTIIEQAPAPEEEPAGEAAE
                     APAAGALPWVLSAKSDAALQAQAERLLSFLESGPGRSPADIGFSLATTRAAWDRRAAVV
                     GESLQELTEGVRALASGNPSAAVIRSTGGLGDKTGFLFSGQGSQRLGMGRELYAAYPVF
                     AAAYDEVCALLDAPVDVDAETLHQTGCTQPALFAVEVALFRLLESWGIRPDYVAGHSVG
                     EIAAAHVAGVLSLQDAAKLVSARAALMQALPSGGAMVAVQATEDEVLPHLTDGVGIAAI
                     NGPQSVVVSGAEEAVLAIAEVFKQQGRKTSRLKVSHAFHSPLMDPMLEDFAEVVRGLTF
                     SEPRIPVVSNLTGRLAEPYTPDYWVRHVREAVRFADGIRTLHDLGVTTFVEIGPGGVLS
                     GMAQGCLDGDDVVTVPLLRADRPEQQAVTAALAELHVHGVSPDWYAIFPGARKADLPTY
                     AFQQQRYWLEAPEAEAAVVDAVDAEFWASVEREDAQSLAATLGLSAEELDVVVPKLSAW
                     RRQRREQSVVDGWRYQLTWQPLDGLPATDLSGSTWLFAAPEGDEWAATVRAALTGRGAH
                     LIPLTVGADAGRESLAGELTRAAAEAGSLQAGSLQGVLSLLATDETASPEHPAVSQGLA
                     RTVALAQALGDAGIDAPLWCVTRGAAATGRSDDAPLSAVQNQVWGFGRALALEHARRWG
                     GLIDLPAAVDERAAGRIAAVLNQGAGTAEDQVAVRASGVFTARLTHARSGTGRPWSPRG
                     TVLITGGTGALGGHVARWLAGAGAEHLVLAGRRGADAPGAAGLKAELEELGARVTLAAC
                     DVADRDAVAALLAEHTFTSVFHAAGVEQFAPFDELTPADFARTMAAKAGGAAHLDELLG
                     DQELDAFVLFSSIAGVWGSGLQTAYAAANAFLDGLAARRRAAGLTATAIAWGPWADGGM
                     VTDADEEHLRRRGVVTLPAALAVTAVQRALDCDDTAAVVADIDWERFIGPFTLGRPSAL
                     LSELPEVRRAEAAAPADAAAGGSAPLVAQLAGLPEAKRTETLIDLVRAHVAAVLGHASA
                     AEVEPGRAFKDLGFDSLTAVELRNKVNTATGLALPPTLVFDYPNATALARFLQSELLGS
                     QAAALQEQHAAAADDEPIAIVAMSCQLPGGVDSPEALWNLVASGGDAISEFPSDRGWDV
                     DSLYDPDPDRPGKTYARDGGFLHDATDFDAAFFGISPREA"
     source          1..8058
                     /altitude="660 m"
                     /collection_date="2010-07-28"
                     /country="China: Xi'an, Shanxi Province"
                     /db_xref="taxon:1488406"
                     /isolation_source="soil"
                     /lat_lon="34.0578 N 109.37750000 E"
                     /mol_type="genomic DNA"
                     /organism="Streptomyces roseifaciens"
                     /strain="MBT76"
                     /submitter_seqid="scaffold_11"
                     /type_material="type strain of Streptomyces roseifaciens"
     region          1..8058
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="T1PKS"
                     /region_number="1"
                     /rules="cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or
                     hyb_KS or itr_KS or tra_KS))"
                     /tool="antismash"
     cand_cluster    1..8058
                     /SMILES="CC(O)C(=O)O"
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="cds(PKS_AT and (PKS_KS or ene_KS or
                     mod_KS or hyb_KS or itr_KS or tra_KS))"
                     /kind="single"
                     /product="T1PKS"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..8058
                     /aStool="rule-based-clusters"
                     /category="PKS"
                     /contig_edge="True"
                     /core_location="[<0:4857](-)"
                     /cutoff="20000"
                     /detection_rule="cds(PKS_AT and (PKS_KS or ene_KS or mod_KS
                     or hyb_KS or itr_KS or tra_KS))"
                     /neighbourhood="20000"
                     /product="T1PKS"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(<1..4857)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="cds(PKS_AT and (PKS_KS or ene_KS or mod_KS
                     or hyb_KS or itr_KS or tra_KS))"
                     /neighbourhood="20000"
                     /product="T1PKS"
                     /protocluster_number="1"
     aSDomain        complement(307..522)
                     /ASF="found active site serine: True"
                     /ASF="non-beta-branching ACP"
                     /aSDomain="ACP"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_AS857_RS36680_ACP.1"
                     /evalue="9.70E-32"
                     /label="AS857_RS36680_ACP.1"
                     /locus_tag="AS857_RS36680"
                     /protein_end="1517"
                     /protein_start="1445"
                     /score="101.1"
                     /tool="antismash"
                     /translation="DLVRAHVAAVLGHASAAEVEPGRAFKDLGFDSLTAVELRNKVNTA
                     TGLALPPTLVFDYPNATALARFLQSEL"
     aSModule        complement(307..4797)
                     /complete
                     /domains="nrpspksdomains_AS857_RS36680_PKS_KS.1"
                     /domains="nrpspksdomains_AS857_RS36680_PKS_AT.1"
                     /domains="nrpspksdomains_AS857_RS36680_PKS_KR.1"
                     /domains="nrpspksdomains_AS857_RS36680_ACP.1"
                     /locus_tags="AS857_RS36680"
                     /monomer_pairings="mal -> ohmal"
                     /tool="antismash"
                     /type="pks"
     aSDomain        complement(838..1362)
                     /ASF="KR domain putatively catalyzing L-configuration
                     product formation"
                     /ASF="catalytic triad S,Y,N inconclusive"
                     /aSDomain="PKS_KR"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_AS857_RS36680_PKS_KR.1"
                     /evalue="2.30E-58"
                     /label="AS857_RS36680_PKS_KR.1"
                     /locus_tag="AS857_RS36680"
                     /protein_end="1340"
                     /protein_start="1165"
                     /score="189.1"
                     /specificity="KR activity: active"
                     /specificity="KR stereochemistry: A1"
                     /tool="antismash"
                     /translation="GTVLITGGTGALGGHVARWLAGAGAEHLVLAGRRGADAPGAAGLK
                     AELEELGARVTLAACDVADRDAVAALLAEHTFTSVFHAAGVEQFAPFDELTPADFARTM
                     AAKAGGAAHLDELLGDQELDAFVLFSSIAGVWGSGLQTAYAAANAFLDGLAARRRAAGL
                     TATAIAWGPWAD"
     CDS_motif       complement(886..996)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_AS857_RS36680_0013"
                     /evalue="3.60E-18"
                     /label="PKSI-KR_m4"
                     /locus_tag="AS857_RS36680"
                     /protein_end="1324"
                     /protein_start="1287"
                     /score="58.1"
                     /tool="antismash"
                     /translation="LDAFVLFSSIAGVWGSGLQTAYAAANAFLDGLAARRR"
     CDS_motif       complement(1261..1359)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_AS857_RS36680_0012"
                     /evalue="1.70E-12"
                     /label="PKSI-KR_m1"
                     /locus_tag="AS857_RS36680"
                     /protein_end="1199"
                     /protein_start="1166"
                     /score="39.9"
                     /tool="antismash"
                     /translation="TVLITGGTGALGGHVARWLAGAGAEHLVLAGRR"
     CDS_motif       complement(1435..1521)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_AS857_RS36680_0011"
                     /evalue="1.50E+01"
                     /label="PKSI-AT-mM_m3"
                     /locus_tag="AS857_RS36680"
                     /protein_end="1141"
                     /protein_start="1112"
                     /score="-1.1"
                     /tool="antismash"
                     /translation="PAAVDERAAGRIAAVLNQGAGTAEDQVAV"
     aSDomain        complement(2365..3198)
                     /ASF="Malonyl-CoA specific"
                     /ASF="found active site serine: True, scaffold matched
                     GHGE: True"
                     /aSDomain="PKS_AT"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_AS857_RS36680_PKS_AT.1"
                     /evalue="2.80E-108"
                     /label="AS857_RS36680_PKS_AT.1"
                     /locus_tag="AS857_RS36680"
                     /protein_end="831"
                     /protein_start="553"
                     /score="353.7"
                     /specificity="substrate consensus: mal"
                     /specificity="PKS signature: Malonyl-CoA"
                     /specificity="Minowa: mal"
                     /tool="antismash"
                     /translation="LFSGQGSQRLGMGRELYAAYPVFAAAYDEVCALLDAPVDVDAETL
                     HQTGCTQPALFAVEVALFRLLESWGIRPDYVAGHSVGEIAAAHVAGVLSLQDAAKLVSA
                     RAALMQALPSGGAMVAVQATEDEVLPHLTDGVGIAAINGPQSVVVSGAEEAVLAIAEVF
                     KQQGRKTSRLKVSHAFHSPLMDPMLEDFAEVVRGLTFSEPRIPVVSNLTGRLAEPYTPD
                     YWVRHVREAVRFADGIRTLHDLGVTTFVEIGPGGVLSGMAQGCLDGDDVVTVPLLR"
     CDS_motif       complement(2497..2532)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_AS857_RS36680_0010"
                     /evalue="6.50E-05"
                     /label="PKSI-AT-M_m8"
                     /locus_tag="AS857_RS36680"
                     /protein_end="787"
                     /protein_start="775"
                     /score="16.2"
                     /tool="antismash"
                     /translation="YWVRHVREAVRF"
     CDS_motif       complement(2746..2784)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_AS857_RS36680_0009"
                     /evalue="6.00E-05"
                     /label="PKSI-AT-M_m5"
                     /locus_tag="AS857_RS36680"
                     /protein_end="704"
                     /protein_start="691"
                     /score="16.1"
                     /tool="antismash"
                     /translation="AAINGPQSVVVSG"
     CDS_motif       complement(2773..2817)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_AS857_RS36680_0008"
                     /evalue="5.40E-02"
                     /label="PKSI-ER_m5"
                     /locus_tag="AS857_RS36680"
                     /protein_end="695"
                     /protein_start="680"
                     /score="6.4"
                     /tool="antismash"
                     /translation="VLPHLTDGVGIAAIN"
     CDS_motif       complement(2869..2991)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_AS857_RS36680_0007"
                     /evalue="5.00E-23"
                     /label="PKSI-AT-M_m3"
                     /locus_tag="AS857_RS36680"
                     /protein_end="663"
                     /protein_start="622"
                     /score="73.4"
                     /tool="antismash"
                     /translation="GIRPDYVAGHSVGEIAAAHVAGVLSLQDAAKLVSARAALMQ"
     CDS_motif       complement(3001..3051)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_AS857_RS36680_0006"
                     /evalue="9.00E-04"
                     /label="PKSI-AT-mM_m2"
                     /locus_tag="AS857_RS36680"
                     /protein_end="619"
                     /protein_start="602"
                     /score="12.5"
                     /tool="antismash"
                     /translation="CTQPALFAVEVALFRLL"
     CDS_motif       complement(3148..3201)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_AS857_RS36680_0005"
                     /evalue="1.40E-05"
                     /label="PKSI-AT-mM_m1"
                     /locus_tag="AS857_RS36680"
                     /protein_end="570"
                     /protein_start="552"
                     /score="17.5"
                     /tool="antismash"
                     /translation="FLFSGQGSQRLGMGRELY"
     aSDomain        complement(3520..4797)
                     /ASF="found active site cysteine: True, scaffold matched
                     GSSS: True"
                     /ASF="found active site histidines: True"
                     /aSDomain="PKS_KS"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_AS857_RS36680_PKS_KS.1"
                     /domain_subtypes="Modular-KS"
                     /evalue="2.80E-181"
                     /label="AS857_RS36680_PKS_KS.1"
                     /locus_tag="AS857_RS36680"
                     /protein_end="446"
                     /protein_start="20"
                     /score="595.0"
                     /tool="antismash"
                     /translation="IAIVGMACRYPGGVASPEDLWHLVSSGADAVTGFPADRGWDLDAI
                     HDPEPGRTGRTYVREGGFLHDAADFDPAFFGISPREALVMDPQQRLLLQTSWEAFERAG
                     IDPATARGTRAGVFVGTNGQDHAYLQRPGDSDIEAHLVTANTAAVVSGRISYALGLEGP
                     AVTVDTACSSSLVALHLAAQALRRGECSLALAGGATVMATPNTFIAFSAQRGLSPDGRC
                     RAFSSTADGTGMSEGVGVLLLERLSDARRLGHDVLAVIRGSAVNQDGASNGLTAPNGPS
                     QVRVIQQALENARLTASQVDAVEAHGTATTLGDPIEAEALLATYGQEHPGDRPLWLGSV
                     KSNLGHTQAASGVAGVIKMVMAIRNGVLPQTLHVDEPTSQVDWSAGGVRLLTEAVQWPE
                     SDHPRRAAVSSFGVSGTNAHTIIEQAP"
     CDS_motif       complement(3742..3786)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_AS857_RS36680_0004"
                     /evalue="1.60E-03"
                     /label="PKSI-KS_m6"
                     /locus_tag="AS857_RS36680"
                     /protein_end="372"
                     /protein_start="357"
                     /score="11.1"
                     /tool="antismash"
                     /translation="GSVKSNLGHTQAASG"
     CDS_motif       complement(3847..3891)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_AS857_RS36680_0003"
                     /evalue="2.50E-03"
                     /label="PKSI-KS_m5"
                     /locus_tag="AS857_RS36680"
                     /protein_end="337"
                     /protein_start="322"
                     /score="10.5"
                     /tool="antismash"
                     /translation="VEAHGTATTLGDPIE"
     CDS_motif       complement(3949..3972)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_AS857_RS36680_0002"
                     /evalue="6.10E+01"
                     /label="PKSI-AT-M_m5"
                     /locus_tag="AS857_RS36680"
                     /protein_end="303"
                     /protein_start="295"
                     /score="-2.2"
                     /tool="antismash"
                     /translation="APNGPSQV"
     CDS_motif       complement(4267..4314)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_AS857_RS36680_0001"
                     /evalue="7.90E-07"
                     /label="PKSI-KS_m3"
                     /locus_tag="AS857_RS36680"
                     /protein_end="197"
                     /protein_start="181"
                     /score="20.9"
                     /tool="antismash"
                     /translation="GPAVTVDTACSSSLVA"
     aSDomain        complement(4810..4854)
                     /aSDomain="PKS_Docking_Nterm"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_AS857_RS36680_PKS_Docking_Nterm.
                     1"
                     /evalue="1.30E-03"
                     /label="AS857_RS36680_PKS_Docking_Nterm.1"
                     /locus_tag="AS857_RS36680"
                     /protein_end="16"
                     /protein_start="1"
                     /score="10.2"
                     /tool="antismash"
                     /translation="DLRHTRRQLGELEAK"
     misc_feature    complement(4928..4941)
                     /note="TFBS match to PerR, Peroxide regulator, confidence:
                     weak, score: 13.6"
                     /tool="antismash"
     CDS             complement(4937..>8054)
                     /NRPS_PKS="Domain: PKS_DH (117-267). E-value: 1.3e-43.
                     Score: 141.2. Matches aSDomain:
                     nrpspksdomains_AS857_RS36685_PKS_DH.1"
                     /NRPS_PKS="Domain: PKS_KR (585-765). E-value: 2.1e-59.
                     Score: 192.5. Matches aSDomain:
                     nrpspksdomains_AS857_RS36685_PKS_KR.1"
                     /NRPS_PKS="Domain: ACP (871-942). E-value: 2e-30. Score:
                     96.9. Matches aSDomain: nrpspksdomains_AS857_RS36685_ACP.1"
                     /NRPS_PKS="Domain: PKS_Docking_Cterm (953-1038). E-value:
                     1.2e-16. Score: 53.2. Matches aSDomain:
                     nrpspksdomains_AS857_RS36685_PKS_Docking_Cterm.1"
                     /codon_start=3
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) adh_short"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PP-binding"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1001: short-chain dehydrogenase/reductase SDR"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981854.1"
                     /locus_tag="AS857_RS36685"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="type I polyketide synthase"
                     /protein_id="WP_144440939.1"
                     /sec_met_domain="adh_short (E-value: 1.3e-63, bitscore:
                     201.3, seeds: 230, tool: rule-based-clusters)"
                     /sec_met_domain="PP-binding (E-value: 4.6e-16, bitscore:
                     46.4, seeds: 164, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="VREAVRFADGIRTLHDLGVTTFVEIGPGGVLSALAQGCLDGDDVV
                     TVPALRTDRPEPQAVTLAAGRLHTHGVSPDWQALFPGAQRVALPTYAFQRERYWLEGTA
                     ALDVSSAGLDSSGHPLLGAVVETAGGQDVLFTGRLSPADTPWIVERTVGGVPVLPVAAF
                     VELALSAGRQAGFPHLEQLDVDAPLLLPAEQGAVRIQVQIGAGEDAERRSVQVHSRPAV
                     GGTDEPWVRHVAGELTRSAPAAEFDMVIWPPAGAEPVDVEEHPSLRAAWRAGDELYAEI
                     ALPLAERDRADRYGLHPVLLDAALDTLAPARAGDPGDPGEVLMPYAWSGASVHAPGAGA
                     ARVRIRPVGAGDGDAVAIELADADGAPVASVASVRTRTVSPQQVLASRVAHRDWMFEVD
                     WIEHTAPDAAAPASEAAWVVLGGDGPYADLDALRKAVDDGAVPPAHVLLPCAPSEGEAA
                     PATRELLAGVLSAVQEWVADERFSDARLVVMTRGAVGLEGAAPDLAHAAVTGLVKSAQA
                     EHPDRLLLVDADDADDAVTTGGLDPALAAALAALDEPQAAVRGGRLFVPRLARTAPPEE
                     PAGHAWDPEGTVLLTGATGGLGLSLARHLVAERGARRLLLVSRRGPAAEGIDEVVAALS
                     GLGAHVDVAACDIADRDALAELLASVPAEHPLVAVVHAAAALDDGVITALTPERLDTVL
                     RPKAEGALHLHELTKDLGLSAFVLFSSLAGVLGSAGAAGYAAANAFLDGLARQRRAAGL
                     PGTSLAWGLWTDNGGMGDRITDADRNRMGRSGVVPFTSAEGLALFDAALTVDRPVIVPV
                     RLDVAGLRSMARAGMLPAVFRGLVGDTGGAAEEDATVGRTPALARRLAGMPEADQEKVL
                     LDLVRNQAAAALGHANAARIEPTTAFRDIGFDSLTAVELRNRLKAATGLRLKPTLVFDH
                     PNPVAIARLLHKDLVLDLAAGTPPVLEEIDRLEAQLAALAPEDAVRDKVTARLKALLWK
                     WTDTHQDAAANAAADAGPGTDRDFDLDLDRIDAATDDEMFDLIDQELGSL"
     gene            complement(4937..>8058)
                     /locus_tag="AS857_RS36685"
     aSDomain        complement(4940..5194)
                     /aSDomain="PKS_Docking_Cterm"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_AS857_RS36685_PKS_Docking_Cterm.
                     1"
                     /evalue="1.20E-16"
                     /label="AS857_RS36685_PKS_Docking_Cterm.1"
                     /locus_tag="AS857_RS36685"
                     /protein_end="1038"
                     /protein_start="953"
                     /score="53.2"
                     /tool="antismash"
                     /translation="PVLEEIDRLEAQLAALAPEDAVRDKVTARLKALLWKWTDTHQDAA
                     ANAAADAGPGTDRDFDLDLDRIDAATDDEMFDLIDQELGS"
     aSDomain        complement(5228..5440)
                     /ASF="found active site serine: True"
                     /ASF="non-beta-branching ACP"
                     /aSDomain="ACP"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_AS857_RS36685_ACP.1"
                     /evalue="2.00E-30"
                     /label="AS857_RS36685_ACP.1"
                     /locus_tag="AS857_RS36685"
                     /protein_end="942"
                     /protein_start="871"
                     /score="96.9"
                     /tool="antismash"
                     /translation="LDLVRNQAAAALGHANAARIEPTTAFRDIGFDSLTAVELRNRLKA
                     ATGLRLKPTLVFDHPNPVAIARLLHK"
     aSModule        complement(5228..7702)
                     /domains="nrpspksdomains_AS857_RS36685_PKS_DH.1"
                     /domains="nrpspksdomains_AS857_RS36685_PKS_KR.1"
                     /domains="nrpspksdomains_AS857_RS36685_ACP.1"
                     /incomplete
                     /locus_tags="AS857_RS36685"
                     /tool="antismash"
                     /type="pks"
     aSDomain        complement(5759..6298)
                     /ASF="KR domain putatively catalyzing D-configuration
                     product formation"
                     /ASF="catalytic triad S,Y,N inconclusive"
                     /aSDomain="PKS_KR"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_AS857_RS36685_PKS_KR.1"
                     /evalue="2.10E-59"
                     /label="AS857_RS36685_PKS_KR.1"
                     /locus_tag="AS857_RS36685"
                     /protein_end="765"
                     /protein_start="585"
                     /score="192.5"
                     /specificity="KR activity: active"
                     /specificity="KR stereochemistry: B1"
                     /tool="antismash"
                     /translation="GTVLLTGATGGLGLSLARHLVAERGARRLLLVSRRGPAAEGIDEV
                     VAALSGLGAHVDVAACDIADRDALAELLASVPAEHPLVAVVHAAAALDDGVITALTPER
                     LDTVLRPKAEGALHLHELTKDLGLSAFVLFSSLAGVLGSAGAAGYAAANAFLDGLARQR
                     RAAGLPGTSLAWGLWTD"
     CDS_motif       complement(5807..5911)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_AS857_RS36685_0003"
                     /evalue="3.20E-17"
                     /label="PKSI-KR_m4"
                     /locus_tag="AS857_RS36685"
                     /protein_end="749"
                     /protein_start="714"
                     /score="55.1"
                     /tool="antismash"
                     /translation="AFVLFSSLAGVLGSAGAAGYAAANAFLDGLARQRR"
     CDS_motif       complement(6194..6295)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_AS857_RS36685_0002"
                     /evalue="1.80E-11"
                     /label="PKSI-KR_m1"
                     /locus_tag="AS857_RS36685"
                     /protein_end="620"
                     /protein_start="586"
                     /score="36.6"
                     /tool="antismash"
                     /translation="TVLLTGATGGLGLSLARHLVAERGARRLLLVSRR"
     aSDomain        complement(7253..7702)
                     /ASF="catalytic triad H,G,P inconclusive"
                     /aSDomain="PKS_DH"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_AS857_RS36685_PKS_DH.1"
                     /evalue="1.30E-43"
                     /label="AS857_RS36685_PKS_DH.1"
                     /locus_tag="AS857_RS36685"
                     /protein_end="267"
                     /protein_start="117"
                     /score="141.2"
                     /tool="antismash"
                     /translation="HPLLGAVVETAGGQDVLFTGRLSPADTPWIVERTVGGVPVLPVAA
                     FVELALSAGRQAGFPHLEQLDVDAPLLLPAEQGAVRIQVQIGAGEDAERRSVQVHSRPA
                     VGGTDEPWVRHVAGELTRSAPAAEFDMVIWPPAGAEPVDVEEHPSL"
     CDS_motif       complement(7541..7600)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_AS857_RS36685_0001"
                     /evalue="7.80E-05"
                     /label="PKSI-DH_m2"
                     /locus_tag="AS857_RS36685"
                     /protein_end="171"
                     /protein_start="151"
                     /score="15.3"
                     /tool="antismash"
                     /translation="VGGVPVLPVAAFVELALSAG"
     misc_feature    8023..8037
                     /note="TFBS match to VqsM, Regulator of quorum-sensing
                     signalling systems, confidence: medium, score: 17.68"
                     /tool="antismash"
ORIGIN
        1 ggcctcgcgg gggctgatgc cgaagaaggc ggcgtcgaag tcggtggcgt cgtggaggaa
       61 gccgccgtcg cgcgcgtagg tcttgccggg gcgatcgggg tcggggtcgt agagcgagtc
      121 gacgtcccag ccgcggtcgg acgggaattc cgagatggcg tcaccgccgg acgccacgag
      181 gttccacagg gcctcgggcg agtcgacgcc gccgggcagc tggcagctca tcgccacgat
      241 cgcgatgggc tcgtcgtcgg cggcggccgc gtgctgctcc tgcagcgcgg cggcctgcga
      301 tccgagcagc tcgctctgga ggaagcgggc gagcgccgtg gcgttggggt agtcgaagac
      361 cagtgtgggc ggcagcgcga ggccggtcgc cgtgttgacc ttgttgcgga gttcgacggc
      421 ggtcagggag tcgaagccga ggtccttgaa cgcccggccg ggctcgacct ccgccgccga
      481 ggcgtggccc aggacggccg cgacgtgtgc gcggaccagg tcgatcaggg tctccgtgcg
      541 cttggcctcc ggcagtccgg cgagctgggc caccaggggc gcgctgccgc ccgctgcggc
      601 gtcggccggt gcggcggcct cggcgcggcg cacctcgggc agctcggaca gcagtgcgct
      661 cgggcggccg agggtgaacg gtccgatgaa ccgttcccag tcgatgtcgg cgaccacggc
      721 cgcggtgtcg tcgcagtcca gggcgcgctg gaccgcggtc acggcgagtg cggcgggcag
      781 ggtgacgacg ccgcggcggc gcaggtgctc ctcgtcggcg tcggtgacca tgccgccgtc
      841 ggcccagggg ccccaggcga tcgcggtggc agtcaggccc gcggccctcc gccgggcggc
      901 gaggccgtcg aggaaggcgt tggcggccgc gtaggcggtc tgcagtccgc tcccccacac
      961 gccggcgatc gaggagaaga ggacgaaggc gtccagctcc tggtcgccca gcagctcgtc
     1021 caggtgggcg gcgccgccgg ccttcgccgc catcgtgcgg gcgaagtcgg ccggggtcag
     1081 ctcgtcgaag ggggcgaact gctccacgcc cgcggcgtgg aacacggagg tgaaggtgtg
     1141 ctcggcgagc agcgccgcca ccgcgtcgcg gtcggcgacg tcgcacgcgg cgagggtgac
     1201 gcgcgctccc agctcctcca gctcggcctt gagtccggcc gcgccgggcg cgtcggcacc
     1261 gcggcggccg gcgagcacca ggtgctcggc gccggcaccg gcgagccagc gggcgacgtg
     1321 cccgcccagc gcacccgtac caccggtgat cagcaccgtg ccgcgcggcg accacggccg
     1381 gccggtcccg ctgcgggcgt gggtgagccg ggcggtgaac acgccggacg cacggacggc
     1441 gacctggtcc tccgcagtgc cggcgccctg gttcagcacg gcggcgatac ggccggcagc
     1501 gcgctcgtcg accgccgccg gcaggtcgat caggccgccc cagcgccggg cgtgctccag
     1561 ggcgagggca cggccgaagc cccacacctg gttctgcacc gcgctcagcg gcgcgtcgtc
     1621 cgagcggccg gtggccgccg cgccccgggt gacgcaccac agcggcgcgt cgatgcccgc
     1681 gtcaccgagc gcctgagcaa gcgccacggt ccgtgcgagg ccctgggaga ccgcggggtg
     1741 ctcgggcgag gcggtctcgt cggtcgcgag cagcgacagg acgccctgga gggatccggc
     1801 ctgcagtgat ccggcttcgg ccgcggcccg ggtcagctcg ccggccaggg actcgcgtcc
     1861 ggcgtccgcg ccgaccgtca gcgggatcag gtgcgcgccg cgtccggtga gggccgcgcg
     1921 gaccgtcgcg gcccactcgt cgccctcggg cgcggcgaac agccaggtgc tgccggacag
     1981 gtccgtggcg ggcaggccgt ccagcggctg ccaggtcagc tggtagcgcc agccgtccac
     2041 gacggactgc tcgcgccgct gccgccgcca ggcggacagc ttcgggacga cgacgtccag
     2101 ctcttcggcg ctcaggccca gcgttgcggc gagcgactgg gcgtcctcgc gctcgacgct
     2161 cgcccagaac tcggcgtcga ccgcatccac gacggccgcc tcggcctcgg gggcctccag
     2221 ccagtagcgc tgctgctgga aggcgtacgt gggcaggtcg gccttccgcg cgccggggaa
     2281 gatcgcgtac cagtccgggg agacaccgtg cacgtgcagt tcggcgagcg cggcggtgac
     2341 ggcctgctgc tcggggcggt cggcacgcag cagcgggacg gtgacgacat cgtcgccgtc
     2401 gaggcagccc tgcgccattc cgctcaggac gccgccgggg ccgatctcca cgaacgtcgt
     2461 gacacccagg tcgtgcaggg tgcggatgcc gtcggcgaag cggaccgctt cgcggacgtg
     2521 ccggacccag taatcgggcg tgtagggctc ggcgaggcgt ccggtgaggt tggagaccac
     2581 ggggatccgc ggctcgctga aggtcagacc gcggacgacc tcggcgaagt cctccagcat
     2641 cgggtccatc aacggcgagt ggaaggcatg gctgaccttc aaccgggagg tcttgcgacc
     2701 ctgctgcttg aagacctcgg cgatcgcgag gacagcttcc tccgcgccgg aaaccacgac
     2761 ggactggggc ccgttgatcg ccgcaatgcc gaccccgtcg gtgaggtgcg gcagaacctc
     2821 gtcctcggtc gcctggaccg ccaccatcgc gccaccggag ggcagcgcct gcatcagtgc
     2881 agcgcgggcg gacaccagtt tcgccgcgtc ctgcaacgac agcacacccg ccacatgagc
     2941 cgcagcgatc tcacccaccg agtgaccggc cacgtaatcc ggccgaatcc cccaggactc
     3001 caggagccgg aacagcgcca cctcaacagc gaacaacgca ggctgggtgc acccggtctg
     3061 gtgaagcgtt tcagcatcaa catccaccgg cgcatccagc agcgcacaca cctcgtcgta
     3121 agccgcagcg aacaccgggt acgccgcata cagctcacga cccatcccca gccgctgcga
     3181 accctgaccc gagaacaaga acccggtctt gtccccgagg ccgcccgtgc tccggatcac
     3241 ggccgcggac gggttgcccg acgccagtgc ccgtacgcct tcggtgagtt cctgcaggct
     3301 ctcgccgacg acggccgccc gccggtccca ggcggcgcgg gtcgtggcga gggagaagcc
     3361 gatgtcggcg ggcgaccggc cggggccgct ctccaggaac gagagcaagc gctcggcctg
     3421 ggcctgcagc gccgcgtcgc tcttggccga gagcacccac ggcagcgcgc cggcggcggg
     3481 cgcctcggcg gcctcgccgg cgggctcctc ctccggggcg ggggcctgct cgatgatcgt
     3541 gtgggcgttg gtgccgctga cgccgaacga ggagacggcg gcacggcggg ggtggtccga
     3601 ctcgggccac tgaacggcct cggtcagcag cctgacgccg ccggccgacc agtcgacctg
     3661 ggaggtcggc tcgtccacgt gcagcgtctg cggcagcacg ccgttgcgga tggccatcac
     3721 catcttgatc acaccggcga cgccggaagc cgcctgcgtg tgaccgaggt tggacttgac
     3781 ggatcccagc cacagcggcc ggtcgcccgg gtgctcctgg ccgtacgtgg cgagcagcgc
     3841 ctcggcttcg atcgggtcgc cgagggtggt ggccgtgccg tgcgcctcga ccgcgtcgac
     3901 ctgggaggcg gtcagccgcg cgttctccag cgcctgctgg atgacgcgca cctgcgacgg
     3961 gccgttcggg gcggtgaggc cgttggacgc gccgtcctgg ttgacggcgc tgccgcggat
     4021 gaccgcgagc acgtcgtggc cgaggcggcg ggcgtcggac aggcgctcca gcaggagcac
     4081 gccgacgcct tcggacatgc ccgtgccgtc ggcggtcgac gagaacgcgc ggcagcggcc
     4141 gtcgggcgac aggccgcgct gcgcgctgaa cgcgatgaac gtgttcggcg tggccatcac
     4201 ggtcgcgccg ccggccaggg cgagcgagca ctcgccgcgg cgcagcgcct gggcggccag
     4261 gtggagcgcc accagcgagg aggagcaggc cgtgtcgacg gtgacggccg ggccttcgag
     4321 gccgagggcg tacgagatgc ggccggacac cacggccgcg gtgttcgcgg tgacgaggtg
     4381 ggcctcgatg tcgctgtcgc ccggccgctg gaggtaggcg tggtcctggc cgttggtgcc
     4441 gacgaacact ccggcccggg tgccccgcgc ggttgcgggg tcgatgcccg cgcgttcgaa
     4501 ggcctcccac gaggtctgca gcagcagccg ctgctggggg tccatgacca gggcctcgcg
     4561 gggggagatc ccgaagaacg cggggtcgaa gtcggcggcg tcgtggagga agccgccctc
     4621 gcgtacgtac gtccgtcccg tgcgcccggg ctcggggtcg tggatcgcgt cgaggtccca
     4681 gccgcggtcg gcggggaacc cggtcacggc gtcggcgccc gaggagacca ggtgccacag
     4741 gtcctcgggg gaggcgactc cgcccgggta gcggcaggcc atcccgacga tggcgatggg
     4801 ctcggtgtcc ttcgcctcca gctcacccag ctgcctgcgg gtgtggcgca ggtccaccat
     4861 cacgcgcttg aggtaatccc tgagcttgtc gtcgttcgac atgttttacg tcctcggtag
     4921 cagtgcgtga gaagagttag agagacccga gctcttggtc gatgaggtcg aacatctcgt
     4981 cgtcggtggc ggcgtcgatc cggtcgaggt cgaggtcgaa gtcgcggtcg gtgccggggc
     5041 cggcgtcggc ggcggcgttc gccgcggcgt cctggtgggt gtcggtccac ttccagagca
     5101 gcgccttgag ccgggcggtg accttgtccc gtaccgcgtc ctcgggggcg agcgcggcca
     5161 gctgggcctc cagccggtcg atctcttcga gcaccggcgg tgtcccggcg gcgaggtcga
     5221 ggaccaggtc cttgtgcagg aggcgggcga tcgcgaccgg gttggggtgg tcgaagacga
     5281 gggtgggctt gagccggagg ccggtcgcgg ccttgagccg gttgcggagc tccacggcgg
     5341 tcagggagtc gaagccgatg tcccggaagg ccgtggtggg ctcgatccgg gccgcgttgg
     5401 cgtggccgag ggcggcggcg gcctggttgc gcaccaggtc cagcagcacc ttctcctggt
     5461 ctgcttcggg catcccggcc aggcggcgtg ccagggcggg ggtccggccg acggtcgcgt
     5521 cctcctcggc cgccccgccg gtgtcgccca cgaggccgcg gaagaccgcc ggcagcatgc
     5581 cggcgcgggc catggagcgc agccccgcga cgtccagccg gaccggcacg atcaccggcc
     5641 ggtcgacggt gagggcggcg tcgaacagtg ccaggccttc cgcggaggtg aacggcacga
     5701 ccccggatcg gcccatccgg ttgcggtcgg cgtcggtgat ccggtcgccc atgccgccgt
     5761 tgtccgtcca caggccccac gccagcgagg tgccgggcag gcccgcggcc cggcgctgcc
     5821 gcgccagccc gtccaggaag gcgttggccg ccgcgtagcc cgccgcgccg gcgctgccca
     5881 ggactccggc cagggaggag aagagcacga acgcggagag gccgaggtcc ttggtcagct
     5941 cgtgcaggtg cagcgcccct tcggccttcg gccgcagcac ggtgtccagg cgctccgggg
     6001 tcagggcggt gatcacaccg tcgtccaggg cggccgccgc gtgcaccacg gccacgagcg
     6061 ggtgctcggc cgggaccgag gccagcagct ccgcgagggc gtcccggtcc gcgatgtcgc
     6121 aggcggccac gtcgacgtgc gcgcccagcc cggacagggc ggcgacgacc tcgtcgatgc
     6181 cctcggcggc ggggccgcgg cggctgacca gcagcagtct ccgtgcgccg cgttcggcga
     6241 cgaggtgccg cgcgagcgac agcccgaggc cgccggtcgc cccggtgagc agcacggtcc
     6301 cttcggggtc ccacgcgtgg ccggcgggct cctcgggcgg ggcggtccgg gcgaggcgcg
     6361 gtacgaacag cctgccgccg cgcactgcgg cctgcggctc gtccagagcg gcgagcgccg
     6421 cagccagtgc ggggtccagg ccgccggtcg tcaccgcgtc gtccgcgtcg tccgcgtcga
     6481 cgagcaggag gcggtccggg tgctcggcct gggccgactt caccaggccg gtcaccgcgg
     6541 cgtgcgcgag gtccggcgcc gctccctcca ggccgacggc gccgcgcgtc atcaccacga
     6601 gccgcgcatc ggagaaccgc tcgtcggcga cccactcctg gacggccgac agcacaccgg
     6661 ccagcaactc gcgcgtcgcc ggggcggctt cgccctcgct cggggcgcag ggcaggagga
     6721 cgtgggcggg cgggaccgcc ccgtcgtcga ccgccttgcg gagcgcgtcg aggtcggcgt
     6781 agggaccgtc gccgccgagg accacccatg cggcctcgga ggcgggagca gccgcatcgg
     6841 gagcggtgtg ctcgatccag tcgacctcga acatccagtc ccggtgcgcg acgcgcgagg
     6901 ccagcacctg ctgcggggac acggtccgcg tccgcacgga cgccaccgag gcgacgggag
     6961 caccgtcggc gtcggccagt tcgatcgcca ccgcgtcgcc gtcgccggcg ccgacgggcc
     7021 ggatccgtac gcgcgcggcc ccggctcccg gggcgtgcac ggacgcgccg ctccaggcgt
     7081 agggcatgag cacctcgccg gggtcgccgg ggtcgccggc acgggccggt gccagggtgt
     7141 ccagggcggc gtccagcagt acggggtgga ggccgtagcg gtccgcccgg tcccgctccg
     7201 cgaggggcag tgcgatctcg gcgtacagct cgtccccggc gcgccaggcg gcgcgcaggc
     7261 tcgggtgctc ctcgacgtcc accggctcgg cgcccgccgg cggccagatc accatgtcga
     7321 actccgctgc cggggcgctg cgggtgagct ctccggcaac gtggcggacc cacggctcgt
     7381 cggtaccgcc gacggcaggc cgactgtgga cctgcaccga ccgtcgctcg gcgtcctcgc
     7441 ccgcgccgat ctgcacctgg atacggacgg cgccctgttc tgccggcagc agcagcgggg
     7501 cgtccacgtc cagctgttcc agatgcggga acccggcctg ccggcctgcg gacagtgcga
     7561 gctccacgaa cgcggcgacc gggagcaccg gcacgccgcc gaccgtgcgt tccacgatcc
     7621 agggtgtgtc ggccggggac agccggccgg tgaagagcac gtcctgtccc ccggcggtct
     7681 ccaccaccgc gccgagcagg gggtggccgg aggagtccag ccccgcggag gagacgtcga
     7741 gcgcggccgt gccttcgagc cagtagcgct cacgctggaa ggcgtaggtg ggcagggcga
     7801 cgcgctgggc gccggggaac agcgcctgcc agtccgggga gacgccgtgg gtgtgcagcc
     7861 gaccggcagc gagggtgacc gcctgcggct cggggcggtc cgtacgcagg gcggggacgg
     7921 tgactacgtc gtcgccgtcg aggcagccct gggcgagggc gctgaggacg ccgccgggac
     7981 cgatctccac gaaggtggtc acgcccaggt cgtgcagggt acggatgcca tcggcgaaac
     8041 ggaccgcttc gcggacgt
//
