LOCUS       NZ_LNBE01000008        11512 bp    DNA     linear   CON 29-MAR-2024
DEFINITION  Streptomyces roseifaciens strain MBT76 scaffold_8, whole genome
            shotgun sequence.
ACCESSION   NZ_LNBE01000008
VERSION     NZ_LNBE01000008.1
KEYWORDS    WGS; RefSeq.
SOURCE      Streptomyces roseifaciens
  ORGANISM  Streptomyces roseifaciens
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 178802)
  AUTHORS   Wu,C., Zhu,H., van Wezel,G.P. and Choi,Y.H.
  TITLE     Metabolomics-guided analysis of isocoumarin production by
            Streptomyces species MBT76 and biotransformation of flavonoids and
            phenylpropanoids
  JOURNAL   Metabolomics 12, 90 (2016)
   PUBMED   27073352
REFERENCE   2  (bases 1 to 178802)
  AUTHORS   Wu,C., Du,C., Choi,Y.H. and Van Wezel,G.P.
  TITLE     The complementarity of genome-mining and NMR metabolic-profiling
            facilitates lead-finding
  JOURNAL   Unpublished
REFERENCE   3  (bases 1 to 178802)
  AUTHORS   Wu,C., Du,C., Choi,Y.H. and Van Wezel,G.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2015) Molecular Biotechnology, Institute of
            Biology Leiden, Sylviusweg 72, Leiden 2333BE, Netherlands
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Velvet v. 1.2.06
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 200.0x
            Sequencing Technology             :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_001445655.1-RS_2024_03_28
            Annotation Date                   :: 03/28/2024 03:28:28
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,528
            CDSs (total)                      :: 7,450
            Genes (coding)                    :: 7,245
            CDSs (with protein)               :: 7,245
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 205
            CDSs (without protein)            :: 205
            Pseudo Genes (ambiguous residues) :: 0 of 205
            Pseudo Genes (frameshifted)       :: 77 of 205
            Pseudo Genes (incomplete)         :: 168 of 205
            Pseudo Genes (internal stop)      :: 20 of 205
            Pseudo Genes (multiple problems)  :: 56 of 205
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 13:37:43
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 51143
            Orig. end                         :: 62655
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            LNBE01000008.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..11512
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="1"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..11512
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..11512
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:6512](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..6512
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     misc_feature    625..638
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 11.47"
                     /tool="antismash"
     misc_feature    complement(643..660)
                     /note="TFBS match to ArcA, Repressor of carbon oxidation,
                     confidence: weak, score: 13.98"
                     /tool="antismash"
     gene            complement(697..2583)
                     /locus_tag="AS857_RS36015"
     CDS             complement(697..2583)
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018547891.1"
                     /locus_tag="AS857_RS36015"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="WP_058047695.1"
                     /transl_table=11
                     /translation="MTNAAAPRTPAPAAQQELDLGWDDPHFHRVASASLRQMAGRLPAV
                     LGRAARLGWAADRAATVQILVAQCVAAAAAAVALAATARVMTGLFGSGTAAQHLYAVLP
                     ALTVAAAAAAVRACADATAQYAALRLAPEVTALADERILADTPEVELTAYDRPDFNDAL
                     QAAGTGAQATQDLITDAQVLLSAVAQLIAAAVVLTALHPLLLPLLVLAVLPKGAAAVAA
                     ARIAHRAAHTSLADNRLRYLLRRFSTEPRTAAEIRAGTMAPYLRQWYAHLTDRIRTRHR
                     AAAPRVLLVTLCGAAGSGTMLALTWLTVGALVLGGAMGLAVAATAVVAVRASTAALTSL
                     VMAGARLFRTSLYLQDWQAFLDLAEELRAVRGPLRLGAGGPREIRAHDVSYRHPGAPPG
                     RPALDGVSVTLRSGELVALVGENGSGKTTLSRLLTGLYLPTAGRVTWDGTDLAETDPHD
                     VWSRVGLVPQDYTRWPMAARENITLGQPRGGDDLVHAAARAAGADSVLDGLPGGLDALL
                     AKSFWGGHDLSGGQWQRFAVARAFYREAAVLVLDEPTSAMDPRAEHTVISRFKELAAGR
                     AAVFVTHNLTNTRIADRIVVLAAGRVVEEGTFEELAVGGGLFAELYKLSQDR"
     gene            complement(2663..4093)
                     /locus_tag="AS857_RS40195"
     CDS             complement(2663..4093)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (extras)
                     Sulfotransfer_4: Sulfotransferase domain"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF036807.4"
                     /locus_tag="AS857_RS40195"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="sulfotransferase family protein"
                     /protein_id="WP_058047696.1"
                     /transl_table=11
                     /translation="MSASGRIRTLPGDFSPPGGRPVDLVLRTVGERTTELALRLALENI
                     RPNRVHIAENIRPLSAAVDRILAIDHHEASHVVHLDADCLVLEDMRPFLDANELAYTDC
                     FVHDRFRGRVHCGVHITRADLVRGMRRADVGAFARDWLGPVLRPEAFRRHLARRELQPG
                     VQLKGFRILHDHLQDHADIYAKYALRELRSRDEGSRARLEAAMAQWGDGPDFTVAREAV
                     RHSRERVPHDVTPDQLAARIEELPAVAARETAALGLPPLPPPAMADVYAAAEAFPREER
                     AKVFGLGLPGTGTRSLTTALHELGFDLASFPFGAGDAAALRRGDGRFPALRHYDGLADV
                     FALPHLAELDALHPGARFVLTVRDKEGWLRAAERLWDTGWPEGAPAAERAAQREARRLF
                     QERVCGGHGFDRERLAQVYDAQAARVRAYFARRPGDLLVLDVCGGEGWEKLAPFLGRRT
                     PAVPFPHTRTNPLDLLPR"
     gene            complement(4090..4815)
                     /locus_tag="AS857_RS40200"
     CDS             complement(4090..4815)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (extras)
                     Sulfotransfer_4: Sulfotransferase domain"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF036807.4"
                     /locus_tag="AS857_RS40200"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="sulfotransferase family protein"
                     /protein_id="WP_058047697.1"
                     /transl_table=11
                     /translation="MTTDRAVRPGSVPRPGPGPVPRPGPPSPAARPKVFGIGLSRTGTR
                     SLTVALRTLGFDVAHYPADPGTYAALLAGTARFPLLDHRDGITDITVVPYYQDLDRAWP
                     GAKFILTVREEEGWLASCRTHWKPAIDGKAGRSDVYPQLQRFLRAAVYASHEFDPERFR
                     TVARRHTAEVLRHFAGRPGDLLVLDITAGQGYERLAPFLGVPVPGGPFPHTNAYARVPA
                     EGAAAGGTAGPAASAGGAR"
     misc_feature    complement(4853..4867)
                     /note="TFBS match to OsdR, Development and stress
                     management regulator, confidence: medium, score: 25.37"
                     /tool="antismash"
     misc_feature    complement(4991..5005)
                     /note="TFBS match to MexT, Global virulence regulator,
                     confidence: weak, score: 15.56"
                     /tool="antismash"
     gene            5001..6512
                     /locus_tag="AS857_RS36030"
     CDS             5001..6512
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: YcaO"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF014661.4"
                     /locus_tag="AS857_RS36030"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="YcaO-like family protein"
                     /protein_id="WP_107105767.1"
                     /sec_met_domain="YcaO (E-value: 2.7e-22, bitscore: 73.1,
                     seeds: 127, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTTPHPDAPGDAPSPDGSGRAPRWRPEVFAPFAEGPGVVFARTAA
                     RSPEFGRTSASGAGRVLIGSAAGDDRADVALRARGELLERMGNVLAGRAAESAARWVGD
                     HDGWRRRGVPALDPAELGDGSGGTAGRAVRQLWVPARSALTGAELLVPAGEVFLQHRPP
                     PGCGTTSGAGSTGLGARPDRAAAAAHAALELLERDLIRRSWYGPGAPPPRTVPADDLIP
                     GPVRLLLTALGLRATLLTLPAPAGTECVVVCVHGGAPPGSPGQSFGARCARAGDRAGTV
                     ARAAYEALMVRWSMTSPVALRTWEGWGGRTPPRTALEHALWAFHGHDALGHWTGRGRGG
                     AGDALDGGRSDATGARPDRSGPENRTDGATDGHGDATTPRPGRKGPATQPDGHSDATPP
                     RPRREGPGTRPGDPVDGSGGGPVDAVRAPYGRSACDRRPAEPAEPSGGLAALAAHTGGD
                     VLLVDTDTPGIRAEGVHVVRVIAPGARPLPADDSRMPGALPHPFG"
     gene            6552..7379
                     /locus_tag="AS857_RS36035"
     CDS             6552..7379
                     /EC_number="2.1.1.-"
                     /GO_function="GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /GO_function="GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1248: methyltransferase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018528447.1"
                     /locus_tag="AS857_RS36035"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="WP_063804447.1"
                     /transl_table=11
                     /translation="MTSSGSPDYTAFAARYDSWFAASGTTAATVEALAGLAAAAGPGPA
                     LELGIGTGRIAVPLAARGVEVHGIDASPAMVEQLRAKSGGEHIPVTMGDFSAVGSGADT
                     GAGAGDGAGDGAGDGADTGIPGTYALVYVVSGTFAELPSQDAQVDCFAGAARRLRPGGL
                     FVLDAHVPEALADDRSTGGQVLPTGDGEVVVRFRETDRAAQRYRSHYVVLDKGPDGLGM
                     HRTTVSFRYAAPGELDLMARLAGLRLRERWGDWTGAPFTSSSAYHVSVYERPE"
     gene            7402..8241
                     /locus_tag="AS857_RS36040"
     CDS             7402..8241
                     /EC_number="2.1.-.-"
                     /GO_function="GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /GO_function="GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1089: methyltransferase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF019847.4"
                     /locus_tag="AS857_RS36040"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="WP_058047699.1"
                     /transl_table=11
                     /translation="MDPEAYMAENRRQWEAWTPLKAASAFYGLDDFRRGGMRIRRLEVE
                     EVGDVSGLSLLHLQSHVGLDTLSWARLGARVTGVDFSAEGTATARSLARDVAPSARFLC
                     ANVYDLPRHLDETFDLVYTSHGVLGWLPDLRRWAAVVARFTAPGGRFYLFESHPTAWLF
                     DNRTDSRELAVRYPYFGSGEPLRWRYRSPLAVPEAEADGWEFSWGHALGDIVNALLEAG
                     LAIEFLHEWPFVAWPMFPFLEERDDGWWHLPAGVPAVPLSFSLLARKPAPDAGKDVL"
     gene            complement(8757..8948)
                     /locus_tag="AS857_RS36045"
     CDS             complement(8757..8948)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="AS857_RS36045"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_058047700.1"
                     /transl_table=11
                     /translation="MALLDGMAIDGFTDFTVHGAVLINRRRLSATRSGLPGPGPTGSRS
                     RPRSARGGPPTGHGLGAG"
     gene            complement(<9005..>9460)
                     /locus_tag="AS857_RS38375"
                     /pseudo=""
     CDS             complement(<9005..9460)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003959843.1"
                     /locus_tag="AS857_RS38375"
                     /note="frameshifted; incomplete; partial in the middle of a
                     contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /product="hypothetical protein"
                     /pseudo=""
                     /transl_table=11
                     /translation="MAVWEEVIIHLPESVTLVSLSATVSNAEEFGDWLDTVRGDTEVIE
                     RLQRSRIWTPGRRGPSPPRRGRTSACRRARARRPADVRPLRGGDGPRRPRRRGRRGPAP
                     GGRPDPCPPLRPRSRLRAARREVNPDLLRMARMENSRTILPRRRSRGL"
     gene            <9451..>9706
                     /locus_tag="AS857_RS41750"
                     /pseudo=""
     CDS             9451..>9706
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003947189.1"
                     /locus_tag="AS857_RS41750"
                     /note="frameshifted; incomplete; partial in the middle of a
                     contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /product="hypothetical protein"
                     /pseudo=""
                     /transl_table=11
                     /translation="MRRRQGRPADRRQQRQRRRPGARDDHRGHRLRGAARPAVAARARR
                     RLRGRRPAVPLRRPAAQRRRGPRQGPLDRRGAHGRHPRRG"
     misc_feature    complement(9483..9498)
                     /note="TFBS match to ColR, Phenol-responsive regulator,
                     confidence: weak, score: 17.28"
                     /tool="antismash"
     gene            9916..10473
                     /locus_tag="AS857_RS41755"
     CDS             9916..10473
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:TIGR04267.2"
                     /locus_tag="AS857_RS41755"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="HEXXH motif-containing putative peptide
                     modification protein"
                     /protein_id="WP_058047701.1"
                     /transl_table=11
                     /translation="MRLLQDVWPEMLDELRTVVRQVALLDGMAIDGFTDFTVHGAVLIN
                     RRRLSATRSGLPGPARLAEALVHEGTHNRCNVAGVRAPFLRPAADDTAGPLATPLRPDP
                     RPLSGLFQQTVVLARCVLLHRRTADEAALPAAGTAAVRTRHDRLLTNLRTAVTSLATHR
                     TSLTEHGAGLLRQCATIARDTT"
ORIGIN
        1 ccgagcgtgg tgccgatgtc ggtgggcacg aggtagctga cgccggtccg gcccgcggac
       61 cagccccaca ggggcagcga gaggccggag tccatggggg tctcgctgcc ggcgagcccg
      121 gccgcgggag agttccagaa ggcgtcccgg gcggggatcg acagcccttc gccgcgcggc
      181 agctgcagcg cggcggaggc ggaaccggcg cccgtgacgg gccacttcag ggtgcggtcg
      241 gcggcgccgg tggagcggac ggtgacgaga agccggccct tgtcggcgcg ggcggtgacc
      301 tccaggccgg tgccggggta cgtccagcgg gcgccgtccg cggtggcggt gacggcgccc
      361 ggggcgccgg gggcggtcgc ggccggtgcg gagagctcca cggtgccgcc cgcggccgtg
      421 cgggcggtga cgcgcagggt ggcggtgtcg acggtggcgg tgccgccgcg gacggggatg
      481 tcgacggact ggccgtggac gcggaccgtc tgcgcgggcg cggcggcgtg ggcgggctcg
      541 gcgagggtga tgcacgggag ggcggccgcc agcacgacgg cggcggcgcg ggcggtgcgg
      601 caggaggtga agaggtgcat ggggagttga taacgaacgg aggtaagagg gttctaagaa
      661 cccggcgttg ccgcgacctc ccggccccgc gccgcgtcac cggtcctggg acagtttgta
      721 cagctctgcg aagagtcctc ccccgacggc gagttcctcg aaggtgccct cctccacgac
      781 gcggcccgcg gcgaggacga cgatgcggtc ggcgatgcgg gtgttggtca gattgtgcgt
      841 gacgaacacg gcggcgcgtc ccgcggcgag ctccttgaag cggctgatga cggtgtgctc
      901 ggcgcgcggg tccatcgcgg acgtgggttc gtcgaggacg aggacggcgg cctcgcggta
      961 gaaggcgcgc gccacggcga agcgctgcca ctggccgccg gagaggtcgt ggccgcccca
     1021 gaacgacttg gcgaggaggg cgtccaggcc gcccgggagg ccgtcgagga cgctgtcggc
     1081 gcccgcggcg cgggccgcgg cgtgcaccag gtcgtcgccg ccgcggggct ggcccagggt
     1141 gatgttctcg cgggccgcca tcggccagcg cgtgtagtcc tgcgggacca gcccgacccg
     1201 cgaccagacg tcgtgggggt cggtctcggc gaggtcggtg ccgtcccagg tgacccgtcc
     1261 ggctgtgggc aggtacagcc ccgtcagcag ccgggagagg gtggtcttcc cggagccgtt
     1321 ctccccgacc agcgcgacga gttcgcccga gcgcagggtg acgctgacgc cgtcgagggc
     1381 gggccttccg ggcggggcgc cggggtgacg gtaggagacg tcgtgggcgc ggatctcccg
     1441 ggggccgccg gctccgagcc gcagcgggcc gcgtacggcc ctgagctcct cggccaggtc
     1501 caggaacgcc tgccagtcct ggaggtagag cgaggtgcgg aagagccggg cccccgccat
     1561 gaccagggag gtcagcgcgg ccgtggaggc ccgtacggcg acgaccgcgg tggcggcgac
     1621 cgccaggccc atggcgccgc cgaggacgag cgcgccgacg gtcagccagg tgagggcgag
     1681 catggtgccg gatccggccg cgccgcagag ggtcaccagc agcacgcggg gggcggcggc
     1741 gcggtgccgg gtgcggatcc ggtcggtgag gtgggcgtac cactggcgca ggtacggggc
     1801 catggtgccg gcccggatct cggcggccgt gcgcggctcg gtgctgaagc ggcgcaggag
     1861 gtagcgcagc cggttgtcgg cgaggctggt gtgggcggcg cggtgggcga tgcgggccgc
     1921 ggcgacggcg gcggctccct tgggcagcac ggcgaggacc agcaggggca ggagcagggg
     1981 gtgcagggcg gtgagcacga ccgccgcggc gatgagctgg gcgacggcgg agagcagcac
     2041 ctgggcgtcg gtgatcaggt cctgggtggc ctgggcgccc gtgccagcgg cctgcagggc
     2101 gtcgttgaag tcgggccggt cgtaggcggt cagctcgacc tcgggggtgt cggccaggat
     2161 gcgctcgtcg gccagggcgg tgacctcggg ggccagccgc agggcggcgt actgggccgt
     2221 ggcgtccgcg caggcgcgca cggcggcggc cgcggcggcg acggtgagcg cgggcagcac
     2281 cgcgtacagg tgctgggcgg ccgtgccgga gccgaagagc ccggtcatga cgcgcgcggt
     2341 ggcggcgagc gcgaccgccg cggccgcggc cgccacgcac tgggcgacca gtatctgcac
     2401 cgtggccgcc cggtccgccg cccaccccag ccgggccgcc cggccgagga ccgccggcag
     2461 ccggcccgcc atctgccgca ggctcgcgga ggccacgcgg tggaagtgcg ggtcgtccca
     2521 gccgaggtcg agttcctgct gcgcggcggg ggcgggggtg cgcggggcgg cggcgttcgt
     2581 caccgggtca tggtgccgtc acaccggccg ccgcgtggcg gaactctccg gcccgtggcc
     2641 ctttagggga tgcaggaccg cttcagcggg gcaggaggtc cagcggattc gtcctggtgt
     2701 gcgggaaggg cacggccggg gtccgccggc cgaggaaggg cgcgagcttc tcccagccct
     2761 cgccgccgca gacgtcgagg accagcaggt cgccggggcg gcgcgcgaag tacgcgcgga
     2821 cgcgggcggc ctgggcgtcg tacacctggg ccagccgctc ccggtcgaag ccgtgcccgc
     2881 cgcagacgcg ctcctggaac agccgtcggg cctcgcgctg cgcggcccgc tccgcggcgg
     2941 gtgcaccctc gggccagccg gtgtcccaca agcgctcggc ggcgcgcagc cagccctcct
     3001 tgtcgcgcac ggtcagcacg aaccgggcac cggggtggag ggcgtccagc tcggcgaggt
     3061 gcggcagggc gaagacgtcg gccagcccgt cgtagtggcg cagggcgggg aagcggccgt
     3121 cgccgcggcg cagggcggca gcgtcccccg cgccgaaggg gaacgaggcg aggtcgaagc
     3181 ccagttcgtg cagcgcggtg gtgaggctgc gggtccccgt gccgggcagg ccgagcccga
     3241 agaccttggc ccgctcctcg cgcgggaagg cctcggcggc cgcgtacacg tcggccatgg
     3301 ccgggggcgg cagcggcggc agtccgaggg ccgcggtctc gcgcgccgcg acggcgggca
     3361 gctcttcgat gcgggcggcg agctgatcgg gcgtcacgtc gtgcggcacg cgctcgcggc
     3421 tgtgccggac cgcctcccgc gcgaccgtga agtcggggcc gtcgccccac tgcgccatgg
     3481 cggcctccag gcgcgcccgg gagccctcgt cgcggctgcg cagctcgcgc agcgcgtatt
     3541 tggcgtagat gtcggcgtgg tcctggaggt ggtcgtggag gatgcggaac cccttgagct
     3601 ggacgccggg ctggagctcc cggcgggcga ggtggcggcg gaaggcctcg gggcgcagca
     3661 cggggcccag ccagtcccgc gcgaacgctc cgacgtccgc ccggcgcatg ccccgcacca
     3721 ggtccgcccg ggtgatgtgc acgccgcagt ggacccggcc gcggaagcgg tcgtgcacga
     3781 agcagtcggt gtaggccagt tcgttggcgt ccaggaacgg ccgcatgtcc tccaggacga
     3841 ggcagtcggc gtccaagtgg acgacgtgcg aggcctcgtg gtggtcgatg gcgaggatcc
     3901 ggtccacggc cgcggacaac ggccggatgt tttcggcgat gtggacgcgg ttcgggcgga
     3961 tgttctcgag ggcgaggcgc agggcgagtt cggtggtgcg ctcgccgacc gtccgcagga
     4021 ccaggtcgac ggggcgcccg ccgggcgggg agaagtcccc ggggagggtc cggatccggc
     4081 cggacgcgct caccgcgcac ccccggcgga cgcggcgggc ccggcggtgc cgccggctgc
     4141 ggcgccctcg gccgggaccc tcgcgtaggc gttggtgtgc gggaagggtc cgccggggac
     4201 ggggacgccg aggaagggcg ccagccgctc gtagccctgt ccggcggtga tgtcgaggac
     4261 cagcaggtcg ccggggcggc cggcgaagtg gcggagcacc tcggcggtgt ggcggcgcgc
     4321 gaccgtacgg aaccgctcgg ggtcgaactc gtggctcgcg tagacggcgg cccgcaggaa
     4381 gcgctgcagc tgcgggtaga cgtcgctccg gccggccttg ccgtcgatcg ccggcttcca
     4441 gtgggtgcgg caggaggcca gccagccctc ctcctcgcgg acggtgagga tgaacttggc
     4501 gcccggccag gcccggtcca ggtcctggta gtacggcacg acggtgatgt cggtgatgcc
     4561 gtcgcggtgg tcgagcagcg ggaagcgggc ggtgccggcg aggagcgccg cgtaggtgcc
     4621 cgggtcggcc gggtagtggg ccacgtcgaa gccgagcgtg cgcagggcga cggtcaggga
     4681 ccgggttccg gtgcgcgaca ggccgatgcc gaagaccttc ggccgggcgg cgggggacgg
     4741 cggtccgggg cgggggacgg gacccgggcc cgggcgggga acggatccgg ggcggactgc
     4801 gcggtcggtt gtcatgtgtt cgcggcctcc tcgcggggtg gcgggacggt gcggggccgg
     4861 gcggcccgtg cgccgtcgcc accgtgccgg atcgcacgga attgtcaacg gcgcgccggg
     4921 cggccggaat tcactgccgc gacgccggtt gcgccgggtg ccgttgtgct gacgctgcgc
     4981 ctttggtcca atcgagggcg atgaccacac ctcacccgga cgccccggga gacgcgccgt
     5041 ccccggacgg ctccggacgt gcgccgcgct ggcggcccga ggtgttcgcc ccgttcgccg
     5101 agggcccggg cgtggtcttc gcgcgcacgg ccgcgcggtc accggaattc ggccggacga
     5161 gcgcgagcgg cgcgggccgg gtcctcatcg gcagcgccgc cggcgacgac cgcgccgacg
     5221 tcgcgctgcg tgcccggggc gagttgctgg agcgcatggg caacgtgctg gccggacggg
     5281 ccgccgagag cgcggcgcgc tgggtgggcg accacgacgg ctggcggcgg cgcggggtgc
     5341 ccgccctgga cccggcggag ctcggcgacg gctcgggcgg gacggccggg cgtgcggtgc
     5401 gccagctgtg ggtcccggcc cgctccgcgc tcaccggcgc cgaactgctc gtcccggcgg
     5461 gcgaggtgtt cctccagcac cgcccgccgc ccggctgcgg cacgacgtcg ggggcgggct
     5521 cgacgggcct gggagcccgg cccgaccggg cggccgcggc cgctcacgcc gccctcgaac
     5581 tcctcgaacg ggacctgatc cggcgcagct ggtacgggcc cggtgcgccc ccgccgcgca
     5641 ccgtccccgc cgacgacctg atacccgggc ccgtacggct cctcctcacc gccctgggcc
     5701 tgcgggccac gctcctgacg ctgcccgcac ccgccggcac ggagtgcgtc gtggtctgcg
     5761 tccacggcgg ggcgccgccc ggcagccccg gccagagctt cggcgcccgc tgcgcgcggg
     5821 cgggcgaccg cgcgggcacg gtggccaggg ccgcctacga ggccctgatg gtgcgctgga
     5881 gcatgaccag ccccgtggcc ctgcgcacct gggaaggctg gggcggccgc accccacccc
     5941 gcacggccct ggaacacgcc ctgtgggcct tccacgggca cgacgcgctg gggcactgga
     6001 cggggcgagg gcgtggcggg gcgggggatg cgcttgacgg cgggcggagc gacgcgaccg
     6061 gggcgcggcc ggaccggtcc gggccggaga accggacgga cggggcgacg gatgggcacg
     6121 gcgacgccac gacgccccgg ccgggacgga agggccccgc aacgcaaccg gacgggcaca
     6181 gcgacgccac accgccccgg ccgcggcggg aagggccggg gacgcgacca ggcgaccctg
     6241 tggacgggtc cggcggcggg cccgtggacg cggtacgagc gccgtacgga cggagtgcct
     6301 gcgaccggcg gccggccgaa ccggccgaac cgtccggcgg gcttgccgcg ctcgccgcgc
     6361 acaccggcgg cgacgtgctg ctcgtcgaca ccgacacccc cggcatccgc gcggaaggcg
     6421 ttcacgtggt gcgggtgatc gcccccggtg cccggcccct gcccgccgac gacagccgga
     6481 tgcccggcgc gctcccccat cccttcggct gaccgcccgc cggccggccg accgcgagaa
     6541 agggcccgac catgacctca tccggctcgc cggactacac cgccttcgcc gcccgctacg
     6601 actcctggtt cgccgcgtcc ggcaccaccg cggccaccgt ggaggccctc gccgggctcg
     6661 ccgcggcggc cgggccgggg ccggccctgg agctgggcat cggcaccggc cggatcgcgg
     6721 tgccgctcgc ggcgcgcggc gtggaggtcc acggcatcga cgcctccccg gccatggtgg
     6781 agcagctgcg ggccaagtcc ggcggcgagc acatcccggt gacgatgggg gacttcagcg
     6841 cggtcgggtc cggagccgat accggagccg gagccgggga cggggccggg gatggggccg
     6901 gggatggggc cgataccggc attccgggga cgtacgcgct cgtctacgtc gtcagcggca
     6961 cgttcgccga actgcccagc caggacgccc aggtggactg cttcgcgggc gcggcccggc
     7021 ggctgcgccc cggcggcctg ttcgtcctgg acgcgcacgt gcccgaagcc ctcgcggacg
     7081 accggtccac gggcggccag gtgctgccga ccggggacgg cgaggtcgtc gtgcggttcc
     7141 gggagaccga ccgggcggcg cagcgctacc gctcccacta cgtcgtcctc gacaagggcc
     7201 cggacggcct cggcatgcac cgtacgacgg tgtcgttccg gtacgcggcg ccgggcgagc
     7261 tggacctgat ggcgcgcctc gcggggctgc ggctgcgcga gcgctggggc gactggacgg
     7321 gcgcgccgtt cacgtcgtcc agcgcgtacc acgtctccgt ctacgagcgc ccggagtgac
     7381 ccgggggaag ggagggcggc catggacccc gaggcgtaca tggcggagaa ccggcggcag
     7441 tgggaggcgt ggacgcccct gaaggccgcc tccgccttct acggcctgga cgacttccgc
     7501 cgcggcggca tgcggatccg ccggctggag gtcgaggagg tgggcgacgt gtcgggcctg
     7561 tccctgctgc acctgcagag ccacgtcggg ctggacaccc tctcctgggc ccggctcggg
     7621 gcgcgcgtga ccggcgtcga cttctccgcc gaggggacgg cgacggcgcg ctccctggcc
     7681 cgcgacgtcg caccgtcggc ccggttcctc tgcgccaacg tctacgacct gccgcggcac
     7741 ttggacgaga ccttcgacct cgtctacacc tcgcacgggg tgctcggctg gctgccggac
     7801 ctgcgccggt gggcggcggt cgtcgcgcgg ttcacggcgc cgggcggccg gttctacctc
     7861 ttcgagtccc acccgacggc ctggctcttc gacaaccgca cggacagccg ggaactcgcc
     7921 gtccgctacc cgtacttcgg ctccggcgag ccgttgcgct ggcgctaccg cagcccgctg
     7981 gcggtgccgg aggccgaggc ggacggctgg gagttctcct gggggcacgc cctgggagac
     8041 atagtgaacg cgctgctgga ggcggggctc gccatcgagt tcctgcacga gtggccgttc
     8101 gtggcctggc cgatgttccc cttcctggag gagcgcgacg acgggtggtg gcacctgccc
     8161 gccggcgtcc ccgccgtgcc gctctcgttc tcgctgctgg cccgcaagcc cgcgccggac
     8221 gccgggaagg acgtcctttg agccacggcg gccgtggata cgcggaaacc gctggtcggc
     8281 gggcgggtgc gggcggctcg gaacggcccg ttccaccatg gcgatcacgg tgtcccgcag
     8341 gcaccatgtc agccgggtgc gccggtgttc cgacgcctcg tcgaacgcaa gacccctcac
     8401 cgccgaagga gcggaaatgc cccaggacat cgtagaagtc caggaagtcc cggccgagga
     8461 gctccccgag ctcgccctca tggtcgacat accgttcgag gagtagcccg gccgcgctgc
     8521 gcggaccgca ctccggcggc cgaccgggca cgggtccggt cggccgctcc gtgcgaccgg
     8581 ggcgggcggg ggcgggccgg gggcgggggt ggctacgggg acgaggcagg aggactgcgt
     8641 ggacgtcgaa ccggacctca cgacggcgca cgccgaccag caggcgctgc agcgcctgct
     8701 ggtcggcggg cgggggcggg gcgccccgct ccgggctgcc cggcccggcc cacccgttag
     8761 ccggcgccga gcccgtgccc ggtcggcggt ccgccacggg ccgagcgcgg ccgtgaccgg
     8821 ctgcccgtcg gccccgggcc gggcagcccg gagcgggtcg ccgagagccg cctgcggttg
     8881 atcaggaccg cgccgtggac ggtgaagtcg gtgaatccgt cgatcgccat gccgtccagg
     8941 agcgccactt ggcggaccac ccccacctgc tgcgcatggc ccgcatggag aacagccgca
     9001 cctacaaccc gcgcgagcgc cgccgcggca agatggtgcg gctgttctcc atgcgggcca
     9061 tgcgcagcag gtcggggttg acctcgcgcc gcgcggcgcg caggcgggag cgggggcgaa
     9121 ggggcgggca cgggtccggt cggccgccgg gggccggccc ccggcggccg cggcgccgcg
     9181 gccgccgtgg tccgtctcct cctcgaagag gtcgtacatc cgccggccgg cgagcacgtg
     9241 ctcgccggca ggcggatgta cgacctcttc gaggaggaga cggaccacgg cggccgggcg
     9301 tccagatgcg gctgcgctgg aggcgctcga tgacctcggt gtcgccgcgc acggtgtcca
     9361 gccagtcgcc gaactcctcg gcgttggaga cggtggccga cagggagacc agggtcaccg
     9421 actccgggag gtggatgatg acctcctccc agacggcgcc gccaaggtcg gcctgctgac
     9481 cggcgacaac agcgtcaacg ccgacgcccc ggtgctcgtg atgaccaccg aggtcatcgt
     9541 ctccgaggag cggcccgtcc cgctgtggca gcacgggctc ggcgccggct gcgaggccgc
     9601 cgtccagcag tgcctctacg ccggcctgcg gctcaacgac gacgaggccc gcgccagggt
     9661 ccgctcgatc gtcgaggagc gcacggccgc catccccgac gaggaccccc gccccgcccc
     9721 cgcccgcccc cgaccgcgac cgggcccgcc gcgtggcccg tcgagctcgc cggcccggcg
     9781 gaccaccccc acctgctgcg cagcctgcgg cgcgccctgg cccagctgcc gtcccggccc
     9841 ggggccgacg ggcagccggt cacggccgcg ctcggcccgt ggcggaccgc cgaccgggag
     9901 gccctggagg cggcggtgcg gctgcttcag gacgtgtggc ccgagatgct cgacgaactc
     9961 cgcaccgtgg tccgccaagt ggcgctcctg gacggcatgg cgatcgacgg attcaccgac
    10021 ttcaccgtcc acggcgcggt cctgatcaac cgcaggcggc tctcggcgac ccgctccggg
    10081 ctgcccggcc cggcccggct ggccgaggca ctggtccacg agggcacgca caaccgctgc
    10141 aacgtggcag gcgtgcgggc gccgttcctg cgccccgcgg ccgacgacac ggcgggcccc
    10201 ctggccacgc ccctgcggcc cgacccgcgc ccgctcagcg gactgttcca gcagacggtg
    10261 gtcctggccc ggtgcgtcct cctccaccgc cggacggcgg acgaggctgc gctccctgcc
    10321 gccgggacgg cggcggtacg tacccgccac gaccgcctgc tgacgaacct gcgaacggcg
    10381 gtaacgtccc tggccacgca ccgaacgagc ctgacggagc acggggcggg gctgctgcgg
    10441 cagtgcgcga cgatcgcacg ggacaccacg tagggcctgg ccggcggggc agagccgaac
    10501 tgcgcgcatc cacgcctcgc cgtgacggcg cgaacggatg cctacggcat cgggggggcg
    10561 cacgggcccg gcatcccacc cgtccgcgaa gtcacatccc cccgcgcccc cgcgccgtcc
    10621 tcagcgcatc cctcacgccc cccgctgtca ggcgggaccg ccacgtcgcc cagtgttccc
    10681 accgtgtccc ccagagacgg gggtcctcgt acgggaccca gcccgttgcc cgcggtgggt
    10741 atcgcggtac ccctacgcac aggcgcttcg tgatgacgaa gcgggtttcg tccgtggtgt
    10801 tgggctggga cgcgtggagg tggtcgccgg cgaagaggag gatgtcgccg gggtccagct
    10861 cgatgtcgac cggggcgccg atctgctggc cgggcaggag cgtgtgcccg tcgtgacgga
    10921 gggggcgccg gtacgcgtcg gggtggaaca agagcccgtt gccgctgcgg accgggccga
    10981 cggccatcca gaggttgacg gagttgacgg cggtgttgaa ccaggagtcg acgtgggcgt
    11041 cgcgcggggt caggaagcct tcgagtccgg ccagttcgtc tccccccgcc ccggccccga
    11101 ccgagcgctg cggcggcatg atgcggatcc cgaggtgccc gctgaggtag agccgggctc
    11161 cttgcggttc cagggccctg gccacgtcgt gcaggacggt gcgggagaag ggccgcagcc
    11221 gggtctccag gtcggtgcgg acggcctcga tctgcgcggt gtccgcgtgg cggtgcgcgt
    11281 tttccagcgc cgcggagacc cgggacgcac cggccgccgg cagggcggcg gccacggcgc
    11341 gggcggcctc ccgccggatc gtccccagcg cgccgatgtg gcgcagtccg cccttgacga
    11401 ccacgagggc gccgtcgagg acgtgccgca gggagacggg gatgccgtac gggccgggtt
    11461 cgacggtgtg cagggcggcg ggcccggccg cgggccgggg ggccgcaccg gg
//
