LOCUS       NZ_LNBE01000004        10366 bp    DNA     linear   CON 29-MAR-2024
DEFINITION  Streptomyces roseifaciens strain MBT76 scaffold_4, whole genome
            shotgun sequence.
ACCESSION   NZ_LNBE01000004
VERSION     NZ_LNBE01000004.1
KEYWORDS    WGS; RefSeq.
SOURCE      Streptomyces roseifaciens
  ORGANISM  Streptomyces roseifaciens
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 3642602)
  AUTHORS   Wu,C., Zhu,H., van Wezel,G.P. and Choi,Y.H.
  TITLE     Metabolomics-guided analysis of isocoumarin production by
            Streptomyces species MBT76 and biotransformation of flavonoids and
            phenylpropanoids
  JOURNAL   Metabolomics 12, 90 (2016)
   PUBMED   27073352
REFERENCE   2  (bases 1 to 3642602)
  AUTHORS   Wu,C., Zhu,H., van Wezel,G.P. and Choi,Y.H.
  TITLE     Metabolomics-guided analysis of isocoumarin production by
            Streptomyces species MBT76 and biotransformation of flavonoids and
            phenylpropanoids
  JOURNAL   Metabolomics 12, 90 (2016)
   PUBMED   27073352
REFERENCE   3  (bases 1 to 3642602)
  AUTHORS   Wu,C., Du,C., Choi,Y.H. and Van Wezel,G.P.
  TITLE     The complementarity of genome-mining and NMR metabolic-profiling
            facilitates lead-finding
  JOURNAL   Unpublished
REFERENCE   4  (bases 1 to 3642602)
  AUTHORS   Wu,C., Du,C., Choi,Y.H. and Van Wezel,G.P.
  TITLE     The complementarity of genome-mining and NMR metabolic-profiling
            facilitates lead-finding
  JOURNAL   Unpublished
REFERENCE   5  (bases 1 to 3642602)
  AUTHORS   Wu,C., Du,C., Choi,Y.H. and Van Wezel,G.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2015) Molecular Biotechnology, Institute of
            Biology Leiden, Sylviusweg 72, Leiden 2333BE, Netherlands
REFERENCE   6  (bases 1 to 3642602)
  AUTHORS   Wu,C., Du,C., Choi,Y.H. and Van Wezel,G.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2015) Molecular Biotechnology, Institute of
            Biology Leiden, Sylviusweg 72, Leiden 2333BE, Netherlands
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Velvet v. 1.2.06
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 200.0x
            Sequencing Technology             :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_001445655.1-RS_2024_03_28
            Annotation Date                   :: 03/28/2024 03:28:28
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,528
            CDSs (total)                      :: 7,450
            Genes (coding)                    :: 7,245
            CDSs (with protein)               :: 7,245
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 205
            CDSs (without protein)            :: 205
            Pseudo Genes (ambiguous residues) :: 0 of 205
            Pseudo Genes (frameshifted)       :: 77 of 205
            Pseudo Genes (incomplete)         :: 168 of 205
            Pseudo Genes (internal stop)      :: 20 of 205
            Pseudo Genes (multiple problems)  :: 56 of 205
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 13:37:43
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 2879086
            Orig. end                         :: 2889452
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            LNBE01000004.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10366
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="melanin"
                     /region_number="8"
                     /rules="melC"
                     /tool="antismash"
     cand_cluster    1..10366
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="melC"
                     /kind="single"
                     /product="melanin"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10366
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5366](+)"
                     /cutoff="20000"
                     /detection_rule="melC"
                     /neighbourhood="5000"
                     /product="melanin"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5366
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="melC"
                     /neighbourhood="5000"
                     /product="melanin"
                     /protocluster_number="1"
     gene            complement(1177..3285)
                     /gene="pta"
                     /locus_tag="AS857_RS30195"
     CDS             complement(1177..3285)
                     /EC_number="2.3.1.8"
                     /GO_function="GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="pta"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018102576.1"
                     /locus_tag="AS857_RS30195"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="phosphate acetyltransferase"
                     /protein_id="WP_058046337.1"
                     /transl_table=11
                     /translation="MTRSVYVTGTDRGDGRQVIELGVMELLTRQVDRVGVFRPLVHGDG
                     PDRLFDLLRSRYRLGQSAESGHGMSYAEASALQAERGTDELVSRLVERFHAVARDYEYV
                     LVLGTDYVGSTLPDELALNARLANEFGASVITVVGGQDQTAESVRAEARNAYRAYEALG
                     CDVVALVVNRVAPADREAVAERLSASLPVPCYALPEEAGLSAPTVAQIVRKLNAEVLLG
                     DDAGLARDAKDFVFGGAMLPVFLPALTEGCLVVTPGDRVDLIVGALAAHSAGAPPIAGV
                     LLTLDEKPGPDILALADRLAPGTPVVSVPGGSFPTAAELFAIEGKLDAFSPRKAETALG
                     LFERHVGTAELTNRIAVARSSRVTPMMFEHELIERSRSARRRIVLPEGTEERVLRAADV
                     VLRRDICDLTLLGEEGAIRKRAGELGIDLASAQIIDPQTSPLRERFAEIYAKARAHKGM
                     TVELAHDVVTDVSYFGTLMVQEGLADGMVSGAVHSTAATIRPAFEIIKTAPGAQIVSSV
                     FFMCLADRVLVYGDCAVNPDPNAEQLADIAIQSAATAAQFGVEPRIAMLSYSTGTSGSG
                     ADVDKVRKATEIVRERRPDLKVEGPIQYDAAVDAAVARTKLPGSEVAGKATVLIFPDLN
                     TGNNTYKAVQRSAGAVAVGPVLQGLRKPVNDLSRGALVQDIVTTVAISAIQAQGEAGNG
                     TAPDAPAV"
     misc_feature    complement(3356..3365)
                     /note="TFBS match to ToxT, Regulator of MSHA biosynthesis,
                     confidence: weak, score: 15.59"
                     /tool="antismash"
     gene            3581..4606
                     /locus_tag="AS857_RS30200"
     CDS             3581..4606
                     /EC_number="2.7.1.11"
                     /GO_function="GO:0003872 - 6-phosphofructokinase activity
                     [Evidence IEA]"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /GO_process="GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004983263.1"
                     /locus_tag="AS857_RS30200"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="WP_058046338.1"
                     /transl_table=11
                     /translation="MRIGVLTAGGDCPGLNAVIRSVVHRAVAGHGDEVIGFEDGFKGLL
                     DGRHRKLDLEAVSGILARGGTILGSSRLERARLREACESSKELAREYGIDVLIPIGGEG
                     TLTAARMLSDAGLPVVGVPKTIDNDISSTDRTFGFDTAVGVATEAIDRLKTTAESHQRV
                     MVVEVMGRHAGWIALESGMAGGAHGICLPERAFAVDDLVKMVEERFARGKKFAVICVAE
                     GAHPAEGSMEYAKGAIDQFGHERFAGIGTKLAAELEKRLGKEARPVILGHVQRGGTPTA
                     YDRVLATRFGWHAVEAAHRGDFGKMTALKGTDIRMVPIADAVTELKRVPESRMDEAESV
                     F"
     misc_feature    4893..4907
                     /note="TFBS match to IolR, Regulator of the inositol
                     catabolism, confidence: medium, score: 18.49"
                     /tool="antismash"
     misc_feature    4955..4970
                     /note="TFBS match to RutR, Regulator of the pyrimidine and
                     purine metabolism, confidence: weak, score: 20.18"
                     /tool="antismash"
     misc_feature    complement(4972..4986)
                     /note="TFBS match to IolR, Regulator of the inositol
                     catabolism, confidence: medium, score: 18.75"
                     /tool="antismash"
     misc_feature    complement(4973..4986)
                     /note="TFBS match to LexA_variant_2, Repressor of DNA
                     damage response, confidence: medium, score: 20.57"
                     /tool="antismash"
     gene            5001..5366
                     /locus_tag="AS857_RS30205"
     CDS             5001..5366
                     /GO_function="GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /GO_process="GO:0042438 - melanin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     melanin: melC"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017240684.1"
                     /locus_tag="AS857_RS30205"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="tyrosinase family oxidase copper chaperone"
                     /protein_id="WP_058046339.1"
                     /sec_met_domain="melC (E-value: 8.3e-36, bitscore: 120.8,
                     seeds: 38, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSDLTRRRMLGRTAAAAAGIVLAGPAAQAFAAESGHDHHGNGSGH
                     RPFDEIYEGRRIEGRLTSGSGGHHDFGIAVFVDGRPLHIMPRGNGWISVMNHYEVFASP
                     YEVTRAAVRALRGVNLV"
     gene            5407..6267
                     /locus_tag="AS857_RS30210"
     CDS             5407..6267
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015038020.1"
                     /locus_tag="AS857_RS30210"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="tyrosinase family protein"
                     /protein_id="WP_058046340.1"
                     /transl_table=11
                     /translation="MAVRKNQAKLTKAERRDFVEALLELKRRGGYDKYVEMHDRFVMSD
                     LDDQERTGHVSPSFLPWHRQFLLDFERDLQSVNPAVTLPYWDFTVNRSLSDPVFAPDFL
                     GGNGRERDAQVMDGPFAYGGGRWPIYFKADERPFLMREMGRSKSTPQLPTAATVEAALA
                     APYYDEAPWNSTSKAGFRNLVEGWANGNTGPHNTVHRWVGGHMNTGMSPNDPVFWLLHC
                     FIDKLWSDWQRRHPKAPAYLPVEPVKDIVALDEPMKPWSEKGQRVTPADMIDHRKFYAY
                     DTDAA"
     gene            complement(6351..6773)
                     /locus_tag="AS857_RS30215"
     CDS             complement(6351..6773)
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1135: MarR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004003562.1"
                     /locus_tag="AS857_RS30215"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="MarR family transcriptional regulator"
                     /protein_id="WP_245700575.1"
                     /transl_table=11
                     /translation="MLHDDDVTLYGLFVEAFSRLKPLVHEDLGVPDTWFEVLLRLGRTP
                     GHALRMSDLATAVAFSSGGFTRLADRMEEAGLIRREPDPDDRRAALAVLTEAGEQALDR
                     ALQQHLAHLRTHVSGRLSAADRRALERILRKLRDAG"
     gene            6915..7424
                     /locus_tag="AS857_RS30220"
     CDS             6915..7424
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004003563.1"
                     /locus_tag="AS857_RS30220"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_058046342.1"
                     /transl_table=11
                     /translation="MRKRTLPALGLTVAAAGVVGAALAMSPATAGAATASPAGPRSVTI
                     EAVEKGTSVNFVDVAPSDSALGDQLIGTGDLVGADGRRIGTSSFQGISTDAKPRTAEML
                     TFVYDLGDGNRITTSGAARLNAQGPVFDEQFAITGGTGKYKNARGQVRVLQETVERAKV
                     TFDIQL"
     gene            7520..8137
                     /locus_tag="AS857_RS30225"
     CDS             7520..8137
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014135584.1"
                     /locus_tag="AS857_RS30225"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="NAD(P)H-binding protein"
                     /protein_id="WP_058046343.1"
                     /transl_table=11
                     /translation="MNTPVKITVLGATGMAGSRIVDEALTRGHAVTAVARRHDEQAPAR
                     DGLQRVTADVTDPVLPLEAADVVVSALPARVALPLLPELTVRAAGRRLLFVGGAGSLRL
                     PSGERVIDQPGFREEWKPEASAHVELLDALRALPQDVDWTFLSPSALFLPGERTGTFRL
                     GGDDLLADADGTSRISAEDYAVALLDELETPRHRRQRFTVGY"
     gene            complement(9264..9485)
                     /locus_tag="AS857_RS30230"
     CDS             complement(9264..9485)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014627598.1"
                     /locus_tag="AS857_RS30230"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="DUF397 domain-containing protein"
                     /protein_id="WP_058046344.1"
                     /transl_table=11
                     /translation="MSAPLNLSRTEWLKSSYSNGDGGNCVEWAPTLAAAHGIVPVRDSK
                     IPGGPALTFAPEAWSLFATAVCDGHFRT"
     gene            complement(9482..10309)
                     /locus_tag="AS857_RS30235"
     CDS             complement(9482..10309)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014177219.1"
                     /locus_tag="AS857_RS30235"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="WP_058046345.1"
                     /transl_table=11
                     /translation="MARTPRKLTPDRSARHLFGADLRHRREAAGMTLEKLAEVVRYSRS
                     HLARIETAEHVPPPDLPARLDEVFGTGEHFARLYAIARHEVHPDKYRRFMELQAQARVI
                     DEYAAHVVPGLVQTEGYARALFRVSNPKASPDAIEEMVAARLSRQVLLTAEAPPDLSII
                     LDEAVIRRPVGGPAVMRAQLVSLVELVDTATTTVQVLPFEHGEHALMSGALTLMTLDDD
                     TNVAYVESIATGSLIEDPETVSARRRSYDLLRAYALSPSKSAAFIRAVTEAPS"
ORIGIN
        1 tccacggcca ggcccagccc ctccaggccg gccacggcgg cctcgcgcac gggcgccgcg
       61 ttctcgccga cgccggcggt gaaggccacg gcgtcgacct tgcccaggac cgcgtagtag
      121 gcgccgatgt acttcttgag ccggtgcacg tagatgtcga aggcgagcgc cgcccgctcg
      181 tcgccctcgt cgatgcggcg gcggatctcg cgcatgtcgt tgtcgccgca caggccgatc
      241 aggccgctct tcttgttgag cagcgcgtcg atctcgtcgg tggacatccc ggcgttgcgg
      301 gacaggtgca ggatgaccgc cgggtccagg tcgcccgaac gggtgcccat gaccaggccc
      361 tccagcgggg tcaggcccat ggaggtgtcg acgcaccggc cgccctcgac cgccgaggcc
      421 gaggcgccgt tgcccaggtg caggacgatg acgttgacct cggaggggtc cttgcccagc
      481 agcttcgcgg tctcgcgcga gacgtaggcg tgcgaggtgc cgtggaagcc gtagcggcgc
      541 accatgtggg cgtcggccgt ggccacgtcg atggcgtagc gggcggcggc ctccggcatc
      601 gtcgtgtgga aggcggtgtc gaagacggcg acctgcggca ggtcggggcg cagcgcctgc
      661 gccgtgcgga tgcccgtgac gttggccggg ttgtgcagcg gggcgaccgg cacgagccgc
      721 tcgatctccg ccagcacctc gtcggtgatc acggtggggg cggtgaactt ctggccgccg
      781 tgcaccaccc ggtggccgat cgcggcgagc tccggcgagt ccaggcccag cccgtccgcg
      841 gccagctccg cggcgaccgc ctgcagcgcg gcctcgtggt cggcgatcgg cgcctcctgc
      901 tcgcgcttgg cgccgcccgt ggcgagcggg gtgtgcgcga ggcgggaggt ctcctcgccg
      961 atgcgctcga ccaggcccac ggccagccgg gcgccgtcgg ccatgtcgag cagctggtac
     1021 ttgaccgagg aggagccgga gttgaggacg aggactcggg tggggttggc agtcatgaac
     1081 gctttccggg ttgtgggggt ctcgggggct gggtcttcga cgtcggtggg ggatgccgta
     1141 cgggaccggg cggcggtccc gtacggccta cgggcgtcag acggcgggcg cgtccggcgc
     1201 ggttccgttc ccggcctcgc cctgggcctg gatcgcggag atcgcgaccg tggtcacgat
     1261 gtcctgcacc agcgcgccgc gggagaggtc gttgacgggc ttgcgcaggc cctgcaggac
     1321 cgggccgacg gccacggcgc cggccgagcg ctggacggcc ttgtaggtgt tgttgccggt
     1381 gttgaggtcg gggaagatca gcacggtggc cttgcccgcg acctccgagc cggggagctt
     1441 ggtcctggcg accgcggcgt ccacggcggc gtcgtactgg atcgggccct cgaccttgag
     1501 gtccgggcgg cgctcgcgga cgatctcggt ggccttgcgc accttgtcga cgtccgcgcc
     1561 ggagccggag gtgccggtgg agtacgacag catcgcgatc cgcggctcga cgccgaactg
     1621 ggcggcggtc gccgcggact ggatggcgat gtccgcgagc tgctcggcgt tcgggtcggg
     1681 gttgaccgcg cagtcgccgt agaccagcac ccggtcggcc aggcacatga agaagaccga
     1741 ggagacgatc tgcgcgcccg gcgcggtctt gatgatctcg aaggccgggc ggatggtggc
     1801 ggcggtggag tgcaccgcgc cggagaccat gccgtcggcc aggccctcct ggaccatcag
     1861 ggtgccgaag taggagacgt cggtgaccac gtcgtgcgcg agctcgacgg tcatgccctt
     1921 gtgggcgcgg gccttggcgt agatctcggc gaagcgctcg cgcagcgggg aggtctgcgg
     1981 gtcgatgatc tgcgccgagg ccaggtcgat gcccagctcg cccgcgcgct tgcggatcgc
     2041 gccctcctcg cccaggagcg tgaggtcgca gatgtcgcgg cgcagcacca cgtccgcggc
     2101 gcgcagcacg cgctcctccg tgccctcggg caggacgatg cggcggcggg ccgagcggga
     2161 gcgctcgatc agctcgtgct cgaacatcat cggggtgacg cggctggagc gggccacggc
     2221 gatgcggttc gtcagctccg cggtgcccac gtgccgctcg aacaggccga gggccgtctc
     2281 cgccttgcgc ggcgagaacg cgtcgagctt gccctcgatc gcgaacagct cggccgcggt
     2341 cgggaaggag ccgcccggca ccgagacgac cggggtgccc ggggccagcc ggtcggcgag
     2401 cgccaggatg tccgggcccg gcttctcgtc cagggtcagc agcacgccgg ctatcggcgg
     2461 ggcgccggcg ctgtgcgcgg cgagggcgcc gacgatcagg tccacccggt cgccgggggt
     2521 cacgaccagg cagccctcgg tcagggcggg caggaagacc ggcagcatgg cgccgccgaa
     2581 gacgaagtcc ttggcgtcgc gggccaggcc cgcgtcgtcg ccgagcagca cctcggcgtt
     2641 cagcttgcgg actatctgcg ccacggtcgg cgcggagagc ccggcctcct cggggagggc
     2701 gtagcagggg acgggcaggc tcgccgacag gcgctcggcg acggcctcgc ggtcggccgg
     2761 ggcgacccgg ttgacgacca gggcgacgac gtcgcagccc agggcctcgt aggcgcggta
     2821 ggcgttgcgc gcctcggccc gcacggactc ggccgtctgg tcctggccgc cgacgaccgt
     2881 gatcaccgag gcgccgaact cgttggcgag gcgggcgttg agggcgagct cgtcggggag
     2941 cgtggagccg acgtagtcgg tgccgagcac cagcacgtac tcgtagtcgc gggcgaccgc
     3001 gtggaagcgc tcgaccagcc gcgagacgag ctcgtccgtg ccgcgctcgg cctgcagcgc
     3061 ggaggcctcc gcgtagctca tgccgtggcc ggactccgcg gactgcccca gccggtagcg
     3121 ggaccgcagc aggtcgaaga ggcggtccgg cccgtcgccg tggacgagcg ggcggaagac
     3181 gcccacccgg tccacctgac gggtgaggag ctccatgact cccagctcga tgacctggcg
     3241 cccgtcgccc cggtcggtcc cggtcacgta cacgctgcgc gtcacgcgtg ctctccttgc
     3301 tccatcgccg tgttcttcgg tgttcttcgg tgtttcttcg ccgtgtgctt cggctttaaa
     3361 aaatacccgt tatggtgggc ttgacctctt gacaatacct gcgcccctgg ctaaggcgcg
     3421 cgtcagcaag gcgtccgggc ccgcaggggc ggaggtcagg gccggcgcgg ccccggggtg
     3481 cgccggtacc catcggaccg tgaaacaatc ggcccggctc acatgtacca tccccttacc
     3541 ttcggtaggg gggaccccag agcaggagac acagcacgcg atgcgcatcg gagttctcac
     3601 cgcaggcggc gactgccccg gcctgaacgc tgtcatccgc tcggtggtgc accgcgcggt
     3661 cgccggccac ggtgacgagg tcatcggctt cgaggacggc ttcaagggcc tgctcgacgg
     3721 ccggcaccgc aagctcgacc tggaagcggt cagcggcatc ctcgcccgcg gcggcaccat
     3781 cctcggctcc tcccgcctgg agcgggcccg gctgcgcgag gcctgcgaga gctccaagga
     3841 gctcgcccgc gagtacggca tcgacgtgct catcccgatc ggcggcgagg gcacgctgac
     3901 cgcggcccgg atgctgtcgg acgcgggcct gcccgtcgtc ggcgtcccca agaccatcga
     3961 caacgacatc tcctccacgg accgcacctt cggcttcgac accgccgtcg gcgtggccac
     4021 cgaggcgatc gaccgcctca agaccaccgc cgagtcccac cagcgggtga tggtcgtcga
     4081 ggtcatgggc cggcacgcgg gctggatcgc cctggagtcc ggcatggccg gcggcgccca
     4141 cggcatctgc ctccccgagc gggccttcgc ggtcgacgac ctcgtgaaga tggtcgagga
     4201 gcgcttcgcc cggggcaaga agttcgccgt catctgtgtc gccgagggcg cgcaccccgc
     4261 cgagggctcg atggagtacg cgaagggcgc catagaccag ttcggccacg agcgcttcgc
     4321 cggcatcggc accaagctcg ccgccgagct ggagaagcgc ctgggcaagg aggcccgccc
     4381 ggtcatcctc ggccacgtcc agcgcggcgg cacccccacc gcgtacgacc gcgtcctcgc
     4441 cacccgcttc ggctggcacg ccgtcgaggc cgcccaccgc ggcgacttcg gcaagatgac
     4501 ggccctgaag ggcaccgaca tcaggatggt gccgatcgcc gacgccgtca ccgagctcaa
     4561 gcgcgtgccg gagagccgga tggacgaggc ggagtcggtc ttctaggcct tccgtcccct
     4621 cgactcctac gaggcttcag ccacgaggcc ctcgccgacc aggtcggcga gggcctcgtg
     4681 gcttttcggc tttcggcttt cggctttcgg cttgttggct tttggtgagg catgccacgc
     4741 tttggctgtt gtgccggaaa tcttccggct gtgcgtgctg cctgcgcaaa gttctcccgt
     4801 ggtggccggg gtgcacgccg cccctttccg gaaggcccct ttattaatac agcctcatat
     4861 tggctgtatc tcgccacatg tccggctctt cccgaagatc tgttacgttc atgatcatct
     4921 acgccggtcg gggggtcggg ggagctcgac gagctggacc ccctggtcgg aaggaacgtt
     4981 tcttctgaag ggggacccgg gtgtccgacc tcacccgccg ccgcatgctc ggccgtaccg
     5041 ccgcagctgc cgccggaatc gtgctcgccg gaccggccgc gcaggccttc gccgccgaga
     5101 gcgggcacga ccaccacggc aacggctccg gccaccggcc gttcgacgag atctacgagg
     5161 gccgccgcat cgagggccgg ctcacctccg gcagcggcgg ccaccacgac ttcggcatcg
     5221 ccgtcttcgt cgacggccgt ccgctgcaca tcatgccgcg cggcaacggc tggatcagcg
     5281 tcatgaacca ctacgaggtc ttcgcgtcgc cgtacgaggt gacccgcgcc gccgtgcggg
     5341 cgctgcgcgg cgtcaacctc gtctgagcca ccacctgatc ccgtttccgt aaagggccac
     5401 attctcatgg cagttcgcaa gaaccaggca aagctcacca aggccgagcg gcgcgacttc
     5461 gtggaggcgc tgctggagct caagcgccgc ggcggctacg acaagtacgt cgagatgcac
     5521 gaccgcttcg tgatgagcga cctggacgac caggagcgca ccggccacgt ctcgccgtcg
     5581 ttcctgccct ggcaccggca gttcctgctc gacttcgagc gggacctgca gtccgtcaac
     5641 ccggccgtca cgctcccgta ctgggacttc acggtcaacc gctcgctgtc cgacccggtc
     5701 ttcgcccccg acttcctcgg cggcaacggg cgcgagcgcg acgcccaggt catggacggc
     5761 ccgttcgcct acggcggcgg ccgctggccg atctacttca aggccgacga acgccccttc
     5821 ctgatgcggg agatggggcg gagcaagagc acgccgcagc tgcccacggc cgccacggtc
     5881 gaggccgcgc tggccgcccc gtactacgac gaggcgccct ggaactccac ctccaaggcc
     5941 ggcttccgca acctcgtcga gggctgggcg aacggcaaca ccggcccgca caacaccgtg
     6001 caccgctggg tcggcggcca catgaacacc ggcatgtcgc ccaacgaccc ggtgttctgg
     6061 ctgctgcact gcttcatcga caagctgtgg tccgactggc agcgccggca cccgaaggcg
     6121 cccgcctacc tgccggtcga gccggtcaag gacatcgtcg ccctcgacga gcccatgaag
     6181 ccgtggagcg agaaggggca gcgggtcacc cccgccgaca tgatcgacca caggaagttc
     6241 tacgcgtacg acaccgacgc cgcgtaagcg gcggcggggg cgcgggggga acgggggagc
     6301 gggggagtgg gggagcgggg gcgggccggg catccggtcc gcccccgctg ctatcccgcg
     6361 tcccgcagct tccgcaggat ccgctccagg gcgcgccggt ccgccgccga taggcggccg
     6421 ctgacgtgcg tgcgcaggtg cgcgaggtgc tgctgcagcg cgcggtccag ggcctgctcg
     6481 cccgcctccg tcagcaccgc gagcgcggcc cgccggtcgt ccgggtccgg ctcccggcgg
     6541 atcagccccg cctcctccat ccggtccgcg agccgcgtga agccgccgga gctgaaggcg
     6601 accgcggtcg ccaggtcgct catccgcagg gcgtgcccgg gcgtgcggcc cagccgcagc
     6661 agcacctcga accaggtgtc cgggaccccg aggtcctcgt ggaccagtgg cttcaggcgg
     6721 ctgaacgcct ccacgaacag tccgtacagc gtcacgtcgt cgtcatgcag catgtgctcc
     6781 tccacctcac cagggtccca gacaatcgcc cccacctgca gaaccttccc ttgcagtatc
     6841 tgccgcaaca gacatattgt ctgctgcggc agatatttct gccaccacgg atcgcaacag
     6901 gctggagtac gtccatgcgc aagcgcaccc ttcccgccct gggcctcacc gtcgccgcgg
     6961 ccggtgtcgt cggcgccgcc ctcgccatga gccccgccac ggcgggtgcc gccaccgcct
     7021 ccccggccgg cccgcgctcg gtcaccatcg aggccgtcga gaagggcacc tcggtcaact
     7081 tcgtcgacgt cgccccgtcc gactccgccc tcggcgacca gctgatcggc accggcgacc
     7141 tcgtcggcgc cgacggccgc aggatcggca ccagcagctt ccagggcatc tccaccgacg
     7201 ccaagccgcg caccgccgag atgctgacct tcgtctacga cctcggcgac ggcaacagga
     7261 tcaccacgtc gggcgccgcc cggctgaacg cccagggccc ggtcttcgac gagcagttcg
     7321 ccatcaccgg cggcaccggc aagtacaaga acgcccgcgg ccaggtgcgc gtcctgcagg
     7381 agaccgtgga gcgggcgaag gtcaccttcg acatccagct ctgaccgcgt cccggccgtg
     7441 acgtgcgggc cctcgtgccc accccggccg tgaccgccac gcccgccccg tcgcccccgc
     7501 acttctggga gaaccccgca tgaacacccc cgtcaagatc accgtcctcg gcgccaccgg
     7561 catggccggc agccgcatcg tcgacgaggc gctcacccgc ggccacgccg tcaccgccgt
     7621 cgcccgccgc cacgacgagc aggccccggc ccgcgacggc ctgcagcgcg tgaccgccga
     7681 cgtgaccgac cccgtcctgc cgctcgaggc cgcggacgtc gtcgtcagcg cgctgcccgc
     7741 ccgcgtcgcc ctgccgctgc tgcccgagct gaccgtccgc gcggccggcc gccgcctgct
     7801 cttcgtcggc ggcgccggca gcctgcgcct gccgtccggc gagcgcgtca tcgaccagcc
     7861 cggcttccgc gaggagtgga agcccgaggc gtccgcgcac gtcgagctgc tggacgcgct
     7921 gcgcgccctg ccgcaggacg tcgactggac cttcctcagc ccctccgccc tcttcctgcc
     7981 cggcgagcgc acgggcacct tccgcctcgg cggcgacgac ctgctggccg acgccgacgg
     8041 cacgtcgcgc atctccgccg aggactacgc ggtcgcgctg ctggacgaac tcgagacgcc
     8101 gcggcaccgc cgtcagcgct tcaccgtcgg ctactgagca ccgccgcccg gcgagcgggt
     8161 gcgaggcccc ggaggggagg cctcgcccct ctcgtcacgt gctgaagcac ggcaccgtcc
     8221 ggtgggcgca cttcgccgag catccagccc cgcgagcgta tcggcggggc agtggggtgc
     8281 gggggtgggc tgggttccgt ggttcgtgtg cgggttagaa caggctggca gcggggggag
     8341 gggtgggggg ctcgggtccg gctgcttggc tgtgcggccg gtgggaggct ggccgccgta
     8401 ctcctgccgt cgcggatgcg ggtgcgtgag gagaccggtc tgtccggttg tggctgaggg
     8461 ggagctggct gctgccgcga tgtggctggg cccgtcgtgg gcgcttcgct cggcgcgccg
     8521 ccgttgtggt tgctcgtccg gctgcgggat gcatgtgggt gcggatttca cccagtgtgg
     8581 gcgtggggcg gcggataagt ggcccgggat ggcggccgag cacctcagca gccccctgag
     8641 tgtgctcctg tcacagacga gcccactcag ggacggcctg ccgcccgctg cggcggataa
     8701 gcgggccgag ttgcagactg caggccacct tcagtcccgc tgggcctccc ctgtcacagg
     8761 agcttccacc caggggcggt taactgcccg ccgcggcgga taagcgggcc gagctgcagg
     8821 ctgcaggtca ccttcagcct caccgggcct ctcctgtcac aggaactcgc atccaggggc
     8881 agcctgccgc ccgcggcggc ggataggtgg gcccggtcgt cagttgcaag ccacccgcag
     8941 cctcaccaca cgccctctgt cacaggaacc cctactcagg ggcagtcgcc cgcccggcca
     9001 cgacggtggc gagctgagcg aagcgcccat gacggcagga accacatcgc ggcagctgcc
     9061 agctccccct cagccacaga cgggcagacc ggtctcctca cgcagccgca tccgcgacgg
     9121 caggagtacg gcggccaacc tcccacccac cgcacagcca acacaccgaa cccgagcccc
     9181 ccacctctcc ccccgctgcc agcctgttct aacccgcaca cgaaccacgg aacccagccc
     9241 acccccgcac cccgccctga ggctcaggtt cggaaatggc cgtcgcacac cgcggtcgcg
     9301 aacaaggacc acgcttccgg cgcaaaggtc agcgccgggc cgccgggtat cttcgagtcc
     9361 cgtacaggaa cgatgccgtg ggcggcggcg agggtggggg cccattcgac gcagttgccg
     9421 ccgtcaccgt tgctgtagct ggacttcagc cactccgttc gggagaggtt gaggggagcg
     9481 ctcacgaggg tgcctccgtt acggctcgga tgaaggccgc cgacttgctt ggcgacaagg
     9541 cataggccct cagcagatcg taggaccggc ggcgagcgct gacagtctcc ggatcttcga
     9601 tcagcgagcc ggtggcgatg ctctccacgt aggcgacgtt cgtgtcgtcg tccagtgtca
     9661 tcagcgtcag ggcgccgctc atcagggcat gttccccatg ttcgaacggc agaacctgaa
     9721 cagtcgtcgt cgcggtgtcc acgagctcga ccaggctgac caattgggcc cgcatgacgg
     9781 ccgggccccc gacgggacgt cggatcaccg cctcgtccaa gatgatggac agatcaggcg
     9841 gcgcctccgc cgtcagcagc acctgacggc tcagccgggc cgccaccatc tcctcgatcg
     9901 cgtccggcga agctttcgga ttgctgacgc ggaagagagc ccgtgcgtag ccctccgtct
     9961 ggacgaggcc cggcacgacg tgggcggcgt actcgtcgat cacccgagcc tgggcttgca
    10021 gctccatgaa tctgcggtac ttgtccggat ggacctcatg ccgcgcgatc gcgtagagcc
    10081 tcgcgaagtg ctcgcccgtg ccgaacacct cgtccagccg cgcaggcagg tcaggcggcg
    10141 gcacgtgctc ggccgtctcg atacgcgcca ggtggctgcg gctgtaccgg acgacctcgg
    10201 cgagtttctc cagcgtcatg cccgccgcct cccgccggtg tctgaggtcg gcgccgaaga
    10261 ggtggcgggc ggaccggtcc ggtgtcagct tccgcggtgt gcgcgccatc ccggtaactc
    10321 cttttctcgg gtacccggtt gggaattgaa actctggccg agcgta
//
