LOCUS       NZ_LNBE01000004        21140 bp    DNA     linear   CON 29-MAR-2024
DEFINITION  Streptomyces roseifaciens strain MBT76 scaffold_4, whole genome
            shotgun sequence.
ACCESSION   NZ_LNBE01000004
VERSION     NZ_LNBE01000004.1
KEYWORDS    WGS; RefSeq.
SOURCE      Streptomyces roseifaciens
  ORGANISM  Streptomyces roseifaciens
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 3642602)
  AUTHORS   Wu,C., Zhu,H., van Wezel,G.P. and Choi,Y.H.
  TITLE     Metabolomics-guided analysis of isocoumarin production by
            Streptomyces species MBT76 and biotransformation of flavonoids and
            phenylpropanoids
  JOURNAL   Metabolomics 12, 90 (2016)
   PUBMED   27073352
REFERENCE   2  (bases 1 to 3642602)
  AUTHORS   Wu,C., Zhu,H., van Wezel,G.P. and Choi,Y.H.
  TITLE     Metabolomics-guided analysis of isocoumarin production by
            Streptomyces species MBT76 and biotransformation of flavonoids and
            phenylpropanoids
  JOURNAL   Metabolomics 12, 90 (2016)
   PUBMED   27073352
REFERENCE   3  (bases 1 to 3642602)
  AUTHORS   Wu,C., Du,C., Choi,Y.H. and Van Wezel,G.P.
  TITLE     The complementarity of genome-mining and NMR metabolic-profiling
            facilitates lead-finding
  JOURNAL   Unpublished
REFERENCE   4  (bases 1 to 3642602)
  AUTHORS   Wu,C., Du,C., Choi,Y.H. and Van Wezel,G.P.
  TITLE     The complementarity of genome-mining and NMR metabolic-profiling
            facilitates lead-finding
  JOURNAL   Unpublished
REFERENCE   5  (bases 1 to 3642602)
  AUTHORS   Wu,C., Du,C., Choi,Y.H. and Van Wezel,G.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2015) Molecular Biotechnology, Institute of
            Biology Leiden, Sylviusweg 72, Leiden 2333BE, Netherlands
REFERENCE   6  (bases 1 to 3642602)
  AUTHORS   Wu,C., Du,C., Choi,Y.H. and Van Wezel,G.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2015) Molecular Biotechnology, Institute of
            Biology Leiden, Sylviusweg 72, Leiden 2333BE, Netherlands
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Velvet v. 1.2.06
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 200.0x
            Sequencing Technology             :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_001445655.1-RS_2024_03_28
            Annotation Date                   :: 03/28/2024 03:28:28
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,528
            CDSs (total)                      :: 7,450
            Genes (coding)                    :: 7,245
            CDSs (with protein)               :: 7,245
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 205
            CDSs (without protein)            :: 205
            Pseudo Genes (ambiguous residues) :: 0 of 205
            Pseudo Genes (frameshifted)       :: 77 of 205
            Pseudo Genes (incomplete)         :: 168 of 205
            Pseudo Genes (internal stop)      :: 20 of 205
            Pseudo Genes (multiple problems)  :: 56 of 205
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 13:37:43
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 2122774
            Orig. end                         :: 2143914
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            LNBE01000004.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..21140
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="triceptide"
                     /region_number="7"
                     /rules="((cds(PF04055 and (TIGR04085 or SPASM)) and
                     triceptide_precursor) or triceptide_rSAM)"
                     /tool="antismash"
     cand_cluster    1..21140
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="((cds(PF04055 and (TIGR04085 or SPASM))
                     and triceptide_precursor) or triceptide_rSAM)"
                     /kind="single"
                     /product="triceptide"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..21140
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[10000:11140](-)"
                     /cutoff="5000"
                     /detection_rule="((cds(PF04055 and (TIGR04085 or SPASM))
                     and triceptide_precursor) or triceptide_rSAM)"
                     /neighbourhood="10000"
                     /product="triceptide"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(10001..11140)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="5000"
                     /detection_rule="((cds(PF04055 and (TIGR04085 or SPASM))
                     and triceptide_precursor) or triceptide_rSAM)"
                     /neighbourhood="10000"
                     /product="triceptide"
                     /protocluster_number="1"
     misc_feature    1040..1054
                     /note="TFBS match to VqsM, Regulator of quorum-sensing
                     signalling systems, confidence: medium, score: 17.8"
                     /tool="antismash"
     misc_feature    complement(1174..1189)
                     /note="TFBS match to ColR, Phenol-responsive regulator,
                     confidence: weak, score: 19.02"
                     /tool="antismash"
     misc_feature    complement(1193..1208)
                     /note="TFBS match to FuR_variant_1, Ferric Uptake
                     Regulator, confidence: weak, score: 15.87"
                     /tool="antismash"
     misc_feature    1199..1213
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence:
                     medium, score: 21.1"
                     /tool="antismash"
     misc_feature    complement(1201..1217)
                     /note="TFBS match to HexR, Glucose-responsive regulator,
                     confidence: weak, score: 15.99"
                     /tool="antismash"
     gene            1240..1569
                     /locus_tag="AS857_RS26810"
     CDS             1240..1569
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004945046.1"
                     /locus_tag="AS857_RS26810"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="ArsR family transcriptional regulator"
                     /protein_id="WP_058045781.1"
                     /transl_table=11
                     /translation="MQGEWLPQPAAEDIRLVRVLHALGDPVRLQLLSVYLRGQEFDCDL
                     SGLGLDHLHKSTVSHHMKVLREAGVTSTRVSGRNRFVKLRRVDLDKRFPGLLDALLRGL
                     EGEAS"
     gene            complement(1603..3666)
                     /locus_tag="AS857_RS26815"
     CDS             complement(1603..3666)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003984356.1"
                     /locus_tag="AS857_RS26815"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="ATP-binding domain-containing protein"
                     /protein_id="WP_245700528.1"
                     /transl_table=11
                     /translation="MPAPQVTTHANDPLERERTHLSSSRAALRAMREDVEALDIKDVTA
                     NWVNAAVLERQIEDRITALADLAHTPLFFGRLDYTHAPGADQAEGAEGEQFYIGRRHVH
                     DADGDPMVIDWRAPVSQPFYRASKKEPMDVRMRRRFGYTGGELTAYEDEDLTDTSGADQ
                     ASKLLQAEIERPRVGPMRDIVATIQPEQDEIVRAGIGGTVCVQGAPGTGKTAVGLHRVA
                     YLLYAHRERLARTGTLVIGPNRSFLHYIEQVLPALGELNVAQATVDDLVAHVEVRGTDG
                     AEAARIKGDARMAEVLRRAVRSGVTMPEESVVVVRGSRRWRVPAYEIEEIVRELLDRDI
                     RYGAAREALPQRIAHAVLVRMEQAGEAPDDRVQDAVARNAAVKAAVKAVWPAVDPVKLV
                     LRLLSDAEFLAEHADGVLSAEEQAEIRWAKPARGVKSAKWSAADAVLIDEAADLVARTA
                     SLGHVVLDEAQDLSPMQYRAVGRRCTTGSATILGDLAQGTTPWATASWQEALTHLGKPE
                     AAVEELTQGFRVPREVIAYASRLLPAIAPGLAEATSVREAAGDFAVRAVPGGELDAAVV
                     AACRESLEREGSIGLIAADARIEALGALLAAAGLPHLSPGEETTAEARLTLVPASLAKG
                     LEYDYVVLDEPAAVVAGEPDERTGLRRLYVALTRAVSGLTVLHAEPLPQQLG"
     gene            3986..4126
                     /locus_tag="AS857_RS40495"
     CDS             3986..4126
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009293605.1"
                     /locus_tag="AS857_RS40495"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_173864806.1"
                     /transl_table=11
                     /translation="MNTATVNPDSAADASPLAHALRAIKAFAGAAVSVVVLGQYADGRR
                     A"
     gene            complement(4175..5821)
                     /locus_tag="AS857_RS26820"
     CDS             complement(4175..5821)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003949998.1"
                     /locus_tag="AS857_RS26820"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="DNA repair helicase XPB"
                     /protein_id="WP_058045783.1"
                     /transl_table=11
                     /translation="MSCLIVQSDKTLLLEVDHEQADACRRAIAPFAELERAPEHIHTYR
                     VTPLGLWNARAAGHDAEQVVDALVTYSRYPVPHSLLVDIAETMDRYGRLRLLKHPTHGL
                     VLESTDRPVLEEILRSKKIQPLVGARIDPDTVAVHPSERGQIKQTLLKLGWPAEDLAGY
                     VDGEAHPIELAEDGWALRPYQQQAVEGFWHGGSGVVVLPCGAGKTLVGAGAMAKAKATT
                     LILVTNTVSARQWKHELVRRTSLTEDEIGEYSGTKKEIRPVTIATYQVLTTKRKGIYPH
                     LELFDSRDWGLVVYDEVHLLPAPVFKFTADLQARRRLGLTATLVREDGRESDVFSLIGP
                     KRFDAPWKEIEAQGYIAPADCVEVRVNLTDSERLAYATAETEEKYRYCATTATKRRVTE
                     ALVARHQGEQTLVIGQYIDQLDELGEHLDAPVIKGETSNAQREKLFDAFRQGEISVLVV
                     SKVANFSIDLPEATVAIQVSGTFGSRQEEAQRLGRVLRPKADGHEARFYSVVARDTVDQ
                     DFAAHRQRFLAEQGYAYRIVDADSLLAGEEA"
     gene            complement(6011..8560)
                     /locus_tag="AS857_RS26825"
     CDS             complement(6011..8560)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004945069.1"
                     /locus_tag="AS857_RS26825"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="helicase-associated domain-containing protein"
                     /protein_id="WP_058045784.1"
                     /transl_table=11
                     /translation="MTTPRETGSSGGAARGGPRTLAEELRARPDGALAGLLRARPDLLN
                     PVPGDLTQLATRAGTRASVVRALERLDRFALQAAEALAVAPDPCPYGTLAALMTGDAPG
                     DPRIAEALPRALATLREQALVWGGEDRLRLVRTVRELLAPSPAHPSPTGLGPTVAEATA
                     GMSPGRLQQIIAAAGLPGTHDPVSAVAALTTVFEDRTRMAELLAGAPAEATAVLGRLVW
                     GPPYGTVTADPAPHLRWLLDRGLLLPASPGNVVLPREAALHLRGGRAHRTPEPVAPELA
                     PATPHSARTVDAAAAGQAFTALGTVEDLLAAWESDGPLVLRAGGLSVRDLKRAAAVLDT
                     TEEAAAFWIELAYAAGLLASDGEVDERYAPTPAFDDWLHLPAEQRWARLATAWLAATRT
                     PGLVGSRDAKGRPLNVLAAELDRSPAPEVRHRALALLATLPPGASVTADTLLDRMHWER
                     PPRTPAEPGTEDLRTRLARWALAEAELLGVTNRGALSAPGRALLGPEKPEGPERPERPA
                     GPEGTEPAFPLSDAEQGACAVRAAALLAPLLPEPLDHILLQADLTAVAPGPLRRPLAQV
                     LGVLADVESKGGATVYRFTPASVRRALDAGQTASDLKAFLTEHSRTPVPQPLAYLIDDV
                     ARRHGRLRVGAASSYLRCDDEAMLAEILADRRSAPLRLRRLAPTVLAAQVAPDALLEGL
                     RAMGYAPAAESAEGDVVVTRADARRTPQRTAPAPVPEGPPVPDETLLGAAVRAIRAGDL
                     ASTAERKPVAAPAPADGGLPRTTSADTLATMQAAVLTGAAVWIGYVNAEGAASQRVIAP
                     VRVEGGFVTGYDHTADEVRTYPLHRITGVAELADEHA"
     gene            complement(9096..9779)
                     /locus_tag="AS857_RS26830"
     CDS             complement(9096..9779)
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF024986.4"
                     /locus_tag="AS857_RS26830"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="WP_058045785.1"
                     /transl_table=11
                     /translation="MDHLLFGRARIVVPDLAEPGLYLHRVQSLERAHGSVQDFHYADAD
                     LRDLDHADVQLITGRITNLHTARLRLERVNLHSVEIDACDLGSARWTESKLTRVVFRHC
                     KLLGAALDGLTLDHVLFESCKLDFTAFTKVRAVGPTVFSRCSLSEATFTDCDLSDAVLS
                     DCTLRNTEFNPGRYQRLDLRGNDLSAVRGAASLSRVVIDRSQQTELGQALMAELHVTYS
                     DDLEA"
     gene            complement(10001..11140)
                     /gene="amcB"
                     /locus_tag="AS857_RS26835"
     CDS             complement(10001..11140)
                     /codon_start=1
                     /gene="amcB"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     triceptide: triceptide_rSAM"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PTHR43273"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF04055"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF041718.1"
                     /locus_tag="AS857_RS26835"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="cyclophane-forming radical SAM peptide maturase
                     AmcB"
                     /protein_id="WP_338058280.1"
                     /sec_met_domain="PTHR43273 (E-value: 3.2e-46, bitscore:
                     155.6, seeds: 35, tool: rule-based-clusters)"
                     /sec_met_domain="PF04055 (E-value: 9.9e-12, bitscore: 43.1,
                     seeds: 518, tool: rule-based-clusters)"
                     /sec_met_domain="triceptide_rSAM (E-value: 1.0, bitscore:
                     1.0, seeds: 0, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MVIRVSAPTPAAPTRRLAAVPEAVVMQPTTLCRPLDCTYCYLPFR
                     KAKHLMPLAVAAAVATSVNAWAKEDSSFEVVWHGGEPLSAGREHLAALMAPFRGVKHTV
                     QTNAALLDDAWCAFLLEKDIGVGVSIDGPAEMNSHRITRAGHPAFQVIMRGIERLRRHE
                     IPFDVIAVVSDPDPAQAADFYTFFAGLGCRSLGVNIEEHEGVNTAHHETEQAAEFWEAL
                     TAAWQTNPAVRVREISRVLDFARAVLNDQGAISRGGRSWDPLPTIAYDGGVVLVSPELT
                     GFLDPRLGDFTSGNVLRTDLKTLLSEADARTSWLAEFWRGVDACKATCPYFDFCGGAHP
                     ANRYFEHDGRLDGTRTRYCTASKIALFEGVTRYADAHCH"
     misc_feature    11192..11208
                     /note="TFBS match to GnfM, Regulator of nitrogen-fixation
                     genes, confidence: weak, score: 19.72"
                     /tool="antismash"
     misc_feature    11205..11218
                     /note="TFBS match to ArgR, Regulator of arginine
                     biosynthesis genes, confidence: weak, score: 17.13"
                     /tool="antismash"
     gene            complement(11224..12075)
                     /locus_tag="AS857_RS26840"
     CDS             complement(11224..12075)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018845068.1"
                     /locus_tag="AS857_RS26840"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="WP_058045786.1"
                     /transl_table=11
                     /translation="MRSAPSSAVREARVALGSRLREIRKAGGFKTARAFAARAGWSESK
                     ASRIENGITQPSDDDLWRYAELCQATDTYPDLLATAHNIEEMYVEWRRLQREGLRPVQE
                     AHVPLYERTRRFRIYEPGVIPGLLQTPAYARSIMGRIIAFWDIPDDTEAAVAVRMGRQR
                     VLRQANRQFGILLEEAALRTRFGDAEVMVSQLGHLLTVSVLQQVSLGIVPMTADRTMWP
                     LEGFWIFDDDQVIIELATAQVTVKQPSEVAIYAQMFSELAQIACYGKEARTLIAAAINA
                     LE"
     gene            complement(12098..12613)
                     /locus_tag="AS857_RS26845"
     CDS             complement(12098..12613)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007263713.1"
                     /locus_tag="AS857_RS26845"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="DUF6879 family protein"
                     /protein_id="WP_338058281.1"
                     /transl_table=11
                     /translation="MPTIEELLASAEHSAVHLEMRDTYMPSDPAFIAWQNGTLTDPVGG
                     DVEFQQWLETVWAATGRGVGIRRARVFSVPESDCIRFEHYVSKANAKAGEDIRWLPRRQ
                     ATDIPFPGNDFWVFDNQLVVVLHFTGDGEAAPDWLEPTRDERVLRLCTSAFEAVWERAV
                     PHAEYRPS"
     gene            complement(12600..12827)
                     /locus_tag="AS857_RS26850"
     CDS             complement(12600..12827)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018090441.1"
                     /locus_tag="AS857_RS26850"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_058045788.1"
                     /transl_table=11
                     /translation="MALRRLGKDPESKSGGSPTIYLDEKKDTYLVQGWKVDDMERLAQL
                     DLPRHETVVEIPRRMVQFFLEVQGGKGADD"
     gene            complement(13018..13833)
                     /locus_tag="AS857_RS26855"
     CDS             complement(13018..13833)
                     /EC_number="2.1.-.-"
                     /GO_function="GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /GO_function="GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1089: methyltransferase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004930043.1"
                     /locus_tag="AS857_RS26855"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="WP_058045789.1"
                     /transl_table=11
                     /translation="MAIPQVAPEIIDFYTDTFDEATRLTATADGMLEMARTQELLRRHL
                     PPAPAALLDVGGGPGAHARWLTADGYRVHLVDPVARHVTQAAGTGCTAELGDARHLTAA
                     DGSYDVTLLLGPLYHLLDRADRDQALAEARRVTASGGLVAAAAINRYASLFEHTATTWL
                     DRESVRGAVTDILATGVHEPGRKGFTAAYFHTAPELAEEMRAAGLHDIRVYAVEGPTWS
                     LLKAAEQHTQQPLPLDSPLFRAALAAARAAEPYPDLLAASSHLLATGRC"
     gene            complement(13875..14054)
                     /locus_tag="AS857_RS26860"
     CDS             complement(13875..14054)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014178882.1"
                     /locus_tag="AS857_RS26860"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="DUF397 domain-containing protein"
                     /protein_id="WP_058045790.1"
                     /transl_table=11
                     /translation="MHDIWMKSSYSNAQHNCVEVAALTEGIGIRDSKDTSIPSLHVHPS
                     AWTSFLHEVRSGRL"
     gene            complement(14047..14976)
                     /locus_tag="AS857_RS26865"
     CDS             complement(14047..14976)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010986811.1"
                     /locus_tag="AS857_RS26865"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="WP_338058282.1"
                     /transl_table=11
                     /translation="MNTRCQTQPGRRRPRKAMRVGARIGPTARRIALGTELRRMRSEAG
                     MTLEQAVKGLGFSESHLQRVETGSIPLKQSSHLRSLLKRYDVTDEDHIAALTQLHRESA
                     SQEWTSFASTVHPTVRTFAGVESVARAIKAWHPTLVLGLLQTKRYATAIFEMEKLHDEF
                     TSEYVDHGVRLRMMRKELLTREEDPLHLWAILGEAALRHIVGNAEVMREQYAEIAALSE
                     RENVTVQILRTHEVGYRFDTDFTILDLGEALPSTVQADVPWGSLCMSDKPREVGRFSRR
                     FSSLSATAMSPEKTVRFLQQLSGEVSDA"
     gene            15201..15593
                     /locus_tag="AS857_RS26870"
     CDS             15201..15593
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF039764.3"
                     /locus_tag="AS857_RS26870"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="DUF6415 family natural product biosynthesis
                     protein"
                     /protein_id="WP_058045791.1"
                     /transl_table=11
                     /translation="MPPFPRTAHTPGEITLAPSEPSPVSIGQALREATAMETWLPAPSR
                     LSEVTERLLGYIRETVPAVEAAAGRRPVECPVRRSAFGAVEEARHRAGGGPGDGYASAI
                     SFARGLGKAAGDLMHQQRRLQREGER"
     misc_feature    15835..15848
                     /note="TFBS match to RpoN, Regulator of nitrogen
                     assimilation and virulence, confidence: medium, score:
                     16.73"
                     /tool="antismash"
     gene            15868..16137
                     /locus_tag="AS857_RS26875"
     CDS             15868..16137
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="AS857_RS26875"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_058045792.1"
                     /transl_table=11
                     /translation="MPKWLVMEETVVAEGAAPHQRILRSLSGNKTRDQALVELSRVAKR
                     HRPDCLKSTPCVVGQDDDGSFWILPKNGKGHARCNLRVIEQTHP"
     gene            complement(16208..19504)
                     /locus_tag="AS857_RS26880"
     CDS             complement(16208..19504)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014177500.1"
                     /locus_tag="AS857_RS26880"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_058045793.1"
                     /transl_table=11
                     /translation="MVRGAAEGADPFGTARLRRGVLDAWAASPARFREDANAEEDLALG
                     GYRDRLVVELAQNAADAAARAGVPGRLRLTLHAAAGSRPAVLAAANTGAPMDAAGVESL
                     ATLRASAKRDADKGAGPGGHGSVGRFGVGFSAVLAVSDEPAVVGRSGGVRWSLAEARAL
                     AAREAVRAPALEEEIRRREGHVPLLRLPLPAEGSAPAGYDTVVVLPLRDGAAEDLTSRL
                     LAGVDDALLLTLPGLSEVVVETDGVVRTLTRRQEGPYVLVDDSARGTTARWRVERAAGA
                     LDSALLADRPVEERLRPHWSVMWAVPVGEDGAPVRPGTAPVVHAPTPTDEPLGVPALLV
                     ATFPLEPTRRHAAPGALTDFLTGRAAEAYAQLLRDWHPVGPGTLDLVPSPLAQGALDAE
                     LRRRILDRLPGVAFLPSAAGVPAQETAAPRGGGVEGIPWTEGPDAARQTPEPAAAPDAP
                     PAADTWDDPFARSGPGGPGEPYALRPADAEVVEGAGAETVAVLGELFPSLLPAGLERRP
                     ELRALGVARAPLGEVVDRLAGVERAPSWWQRLYDSLAGIDPDRLTGLPVPLADGRTAIG
                     PRQVLLPPPDGADSERLSRLGLKVAHPDAAHPLLEKLGATPSSPRAVLTTPQVRAAVAA
                     SLDADEVWDEDALDADALAELVLGLVRDADLAPGDEPWLGALALPDEDGELAPAGELVL
                     PGSVFERVIRPGELAACDAALAERWGEQPLTAVGVMAGFALVRATDVVLDPDELEPRDG
                     DFAEPDDAGLLDAVDVWCEDVLDRLPDTPVPPVATEIVAVRDLDLVDDDAWPEALALLA
                     EPPLRDALTTPVRILLPDGTTESVRPYTAWWLRGHPVLDGRRPAGLRVAGGDPLLRGLY
                     EAVDAAGLADEQVLRALGVRTSAAALLDEPGGAAELLSRLADPALPVTAPQLHALYGLL
                     ARLDPDQVTLPDELRAVTDGEVRVVDAADAVVADAPDLLPLATGHALLPVTPSRSADLA
                     DLLQVRRLSETLEASAPAGQGELREVPEGVRVLLPGAPETYVEHEELVVDGVELDWRLG
                     ADGILHASTLEGVAAGLAWAAGAWSRRFEAAALLEDPSRTEELARARWFD"
     misc_feature    complement(19574..19589)
                     /note="TFBS match to AfsQ1, Two-component system AfsQ1-Q2,
                     activator of antibiotic production, confidence: medium,
                     score: 17.28"
                     /tool="antismash"
     misc_feature    complement(19637..19656)
                     /note="TFBS match to DosR, Hypoxia stress response
                     regulator, confidence: medium, score: 23.85"
                     /tool="antismash"
     gene            complement(19853..20743)
                     /locus_tag="AS857_RS26885"
     CDS             complement(19853..20743)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004945121.1"
                     /locus_tag="AS857_RS26885"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="DUF3027 domain-containing protein"
                     /protein_id="WP_058045794.1"
                     /transl_table=11
                     /translation="MRSRTPDRLCAEAVDLARAAAEEAALPGTVGEHVQAVADGDRVVT
                     HSFLCLDPAYRGWRWAVTVARASRAKNVTLDETVLLPGPDALMAPEWVPWSERLRPGDM
                     GPGDLLPTDAEDLRLVPGWSGEDVPPPNSAVSEEMDRLAEAEDADVVPGSPAAQPAVPA
                     RGTIAAVAEELGMRRARVLSRYGLHTAADRWDETHGAKTPMAQAAPASCVSCGFLVPIG
                     GSLGQAFGVCANEFGPADGHLVSLSYGCGGHSEAAVMPKPPRPAPPVIDETLVDPLPLR
                     SEPAGDEEDEELGHS"
ORIGIN
        1 gtcgcagccg gcctcgtcgt cgctgccgac atccccagcg ccaccagcgc cagcgccgac
       61 gcccccagcg ggaaccagcg ctctcccagg agtgccatcg ccaggctgcc cgtcccgccg
      121 cccgcggcca tgcccaggta cagcgcgctg ctgttgaggg agagcagcag cggtgcgtcg
      181 gcggggcgca gggaggccag gcggtggggc tgggccaccg agaccgacca gcctgcgagc
      241 ggcaccacca gcgcgtagac gagcgcgccc gtcatcgtcc ggcccagcca cggcagcgct
      301 gcgaagaaca ccgccgagac cacgctgccg accagcagga ccttgcggac gccccagcgg
      361 tccacgacgt ggccgccgag ccagctgccc gcgaccgagg cgacgcccgt caccagcagg
      421 atggtgctca gccggtccgc gtcgccgccc gtcgccgggt ggacggccgt gcccaggtag
      481 atgtagacgg actggaacgc catgaagaag gcgaaggtcg tcgccgcggc ggccaggacg
      541 cgcccgtcgc gcagcggggc gagccggtcc cgcaggccca ccgtccgggc gggcgcgggc
      601 agttcgcgga ggaagacggc caggcccagc agggccacgg caccgagcgc ggtgacgagc
      661 cacatggtca gccgccagtc cgcccggccc acgtaggtgc cgagcggcac gcccagggcc
      721 gtggccacgg tcatcccgcc gagcacggtc gcgatggccc ggccgcgccg ctccggcggg
      781 acgagggtgg tggcggccgc gctggcgttc gggctgtaga gggcggctcc ggcggcggcc
      841 aggacgcggg tcgcgagaag cgtcgggtag tcgggtgcga gggcgctcag ggcgttcgcg
      901 gcggtgaaca cggcgagtgc cgtgagcagc aggcggcggc gcggccagcg ggcggtcgcg
      961 gcggcgagga ccggggcgag cagcgcgtac gcgagcgcga agaccgtcac cacctggccg
     1021 gccgccagga cggagacgcc gaggtcgtcg gccacccggc cgaggatgcc ggccatgacc
     1081 atggtgtcgg tgcccacggc aaaggtgccg agggcgagca gaagcagtcg cagacgcaca
     1141 ggatccccct tcgagacgca ccgttggatg ctcgtcaaac aattggggat gggtggataa
     1201 ttgtcaaaca attgccgggg ggcgaggagg gaagagcaca tgcagggcga gtggctgccg
     1261 cagcccgcgg ccgaggacat ccggctggtg agagtgctgc acgcgctcgg cgacccggtg
     1321 cgcctgcagc tcctgtcggt ctacttgagg ggccaggagt tcgactgcga cctgagcggg
     1381 ctcggcctgg accacctgca caagtccacg gtgtcgcacc acatgaaggt cctgcgcgag
     1441 gcgggggtga cctcgacgcg cgtctccggc cgcaaccggt tcgtgaagct gcggcgggtg
     1501 gatctcgaca agcggttccc cgggctgctg gacgccctgc tcagggggtt ggagggggag
     1561 gcctcgtagg gcctcccggg caggggtcct gcgcggcggg ggtcacccca actgctgtgg
     1621 cagcggctct gcgtgaagca ccgtcagccc cgacaccgca cgcgtcagcg ccacgtacag
     1681 ccggcgcagc cccgtccgct cgtccggctc gcctgcgacg accgccgccg gctcgtccag
     1741 gaccacgtag tcgtactcca ggcccttcgc cagcgaggcc ggcaccagcg tcaggcgggc
     1801 ctccgccgtc gtctcctcgc ccggggacag gtgcggcagg cccgccgccg ccagcagcgc
     1861 gcccagcgcc tcgatccgcg cgtccgccgc gatcaggccg atcgacccct cccgctccag
     1921 cgactcccgg cacgcggcca cgaccgccgc gtccagctcc ccgccgggca ccgcgcgcac
     1981 cgcgaagtcg cccgccgcct cacggacgga cgtggcctcg gccaggccgg gggcgatggc
     2041 gggcagcagc cgcgacgcgt aggcgatcac ctcgcgcggc acgcggaagc cctgcgtgag
     2101 ctcctccacc gcggcctccg gcttgccgag gtgcgtgagc gcctcctgcc aactggccgt
     2161 cgcccacggc gtcgtgccct gcgcgaggtc gccgaggatc gtcgcggaac cggtcgtgca
     2221 gcgccggccc accgcgcggt actgcatggg ggagaggtcc tgggcctcgt ccaggaccac
     2281 gtggcccagc gacgccgtcc gcgccaccag gtccgccgcc tcgtcgatca gcaccgcgtc
     2341 cgccgccgac cacttcgccg acttcacgcc gcgcgccggc ttcgcccacc ggatctccgc
     2401 ctgctcctcc gccgagagca ccccgtccgc gtgctccgcc aggaactccg cgtcggacag
     2461 cagccgcagc accagcttca ccggatcgac cgcgggccac accgccttga cggccgcctt
     2521 cacggcggcg ttgcgtgcga ccgcgtcctg cacccggtcg tccggcgcct cgcccgcctg
     2581 ctccatgcgc accagcacgg cgtgcgcgat ccgctgcggc agggcctcgc gggcggcgcc
     2641 gtagcggatg tcgcggtcca ggagctcgcg gacgatctcc tcgatctcgt acgcgggcac
     2701 ccgccaccgc cgcgagccgc gcacgacgac cacggactcc tccggcatcg tcaccccgga
     2761 gcgcacggcg cgccgcagca cctccgccat gcgggcgtcg cccttgatgc gggcggcctc
     2821 cgcgccgtcc gtgccgcgca cctccacgtg cgcgacgagg tcgtcgaccg tcgcctgcgc
     2881 cacgttcaac tcgcccagcg cgggcagaac ttgctcgatg tagtgcagga acgaccggtt
     2941 cggtccgatg accagcgtgc ccgtgcgggc gaggcgctcg cggtgcgcgt agagcagata
     3001 ggccacacgg tgcaggccga ccgcggtctt cccggtgccg ggagccccct ggacgcacac
     3061 cgtgccgccg atccccgcac ggacgatctc gtcctgctcc ggctggatcg tggccacgat
     3121 gtcccgcatg gggcccacgc gcggccgctc gatctccgcc tgcagcagct tgctggcctg
     3181 gtcggcgccg gacgtgtcgg tgaggtcctc gtcctcgtac gcggtgagtt cgccgcccgt
     3241 gtagccgaag cgacggcgca tccgcacgtc catcggctcc ttcttggacg cccggtagaa
     3301 cggctgcgag acgggcgcgc gccagtcgat caccatcggg tcgccgtccg cgtcgtgcac
     3361 gtggcggcgg ccgatgtaga actgctcgcc ctcggcccct tcggcctggt ccgcgcccgg
     3421 cgcgtgcgtg tagtccaagc gcccgaagaa cagcggggtg tgcgcgagat cggccagcgc
     3481 cgtgatgcgg tcctcgatct gccgctccag gacggcggcg ttgacccagt tcgcggtgac
     3541 gtccttgatg tccagggcct cgacgtcctc ccgcatcgcg cgcagggcgg cgcgggagga
     3601 ggagaggtgg gtgcgctccc gctccagggg gtcgttcgcg tgggtggtca cttgcggcgc
     3661 gggcacggca gagcctccag acgtacgggg gacgatcgga agccgccggt ttccgtccgg
     3721 tcggcggccg cgctccgtgc ggtggacgct gcgggaggcg ggcgggaagg ggagattcta
     3781 gtcagcgggc cgggggcgtg cgagcgattt tcccgcgctc cccaggggcg tttcagggtc
     3841 gtccccgggg ccgcccccag ggacgtcctc agggtcggcg tccacctccc gtaggacgcc
     3901 atgatcctcg ggtcgtacgc gcgggcctgc gcttttacac cggcaggccg atgtccgggg
     3961 cgcggcgggg aaccatcatg gagacatgaa cacagcaacc gtgaacccgg actccgccgc
     4021 cgacgcgtcc cccctcgcgc acgccctccg tgcgatcaag gccttcgccg gcgcggccgt
     4081 cagcgtcgtc gtcctcgggc agtacgcgga cggccgcagg gcctgacccc ggctctcctc
     4141 cgtacgaggc cggggtcgtg cgcggccgta cggcttacgc ctcctcgccc gcgagcagcg
     4201 agtccgcgtc cacgatgcgg tacgcgtaac cctgctcggc caggaaccgc tgccggtgcg
     4261 ccgcgaagtc ctggtcgacg gtgtcccggg cgaccaccga gtagaagcgc gcctcgtgcc
     4321 cgtccgcctt cgggcgcagc acccggccga ggcgctgtgc ctcctcctgc cgggagccga
     4381 acgtgcccga cacctggatg gcgaccgtcg cctccggaag gtcgatggag aagttcgcga
     4441 ccttcgagac cacgagcacc gagatctcgc cctgccggaa ggcgtcgaag agcttctcgc
     4501 gctgggcatt gctggtctcg cccttgatca cgggcgcgtc gaggtgctcg ccgagctcgt
     4561 cgagctggtc gatgtactgc ccgatgacca gcgtctgctc gccctggtgc cgggccacca
     4621 gcgcctccgt gacgcgccgc ttcgtggcgg tcgtcgcgca gtagcggtac ttctcctccg
     4681 tctcggccgt cgcgtaggcg agccgctcgg agtccgtgag gttgacgcgc acctccacgc
     4741 agtcggccgg cgcgatgtag ccctgcgcct cgatctcctt ccacggcgcg tcgaagcgct
     4801 tcgggccgat gagggagaag acgtcggact cgcgcccgtc ctcgcgcacg agcgtcgccg
     4861 tcaggccgag ccgccgccgc gcctgcaggt cggccgtgaa cttgaagacc ggcgccggca
     4921 gcaggtgcac ctcgtcgtag acgaccaggc cccagtcgcg ggagtcgaac agctccaggt
     4981 gcgggtagat gcccttccgc ttggtcgtga ggacctggta cgtggcgatg gtgaccggcc
     5041 ggatctcctt cttcgtaccg ctgtactcgc cgatctcgtc ctcggtcagc gacgtgcgcc
     5101 tcaccagctc gtgcttccac tgccgcgccg agaccgtgtt cgtgacgagg atcagcgtgg
     5161 tcgccttggc cttggccatc gcaccggcgc ccacgagcgt cttgcccgcg ccgcagggca
     5221 gcacgaccac gcccgaaccg ccgtgccaga acccctcgac ggcctgctgc tggtacggcc
     5281 gcagcgccca gccgtcctcc gccagctcga tcgggtgcgc ctcgccgtcc acgtacccgg
     5341 ccaggtcctc cgccggccag cccagcttca gcagcgtctg cttgatctgc ccgcgctcgg
     5401 aggggtgcac ggcgaccgtg tccgggtcga tccgggcgcc gacgagcggc tggatcttct
     5461 tcgagcggag gatctcctcc aggacggggc ggtccgtcga ctccaggacc agcccgtgcg
     5521 tggggtgctt gagcagccgc agccgtccgt agcggtccat cgtctccgcg atgtcgacga
     5581 ggagggagtg cgggacgggg tagcgcgagt acgtgacgag ggcgtcgacg acctgctcgg
     5641 cgtcgtgccc ggccgcccgg gcgttccaca ggcccagcgg ggtgacccgg taggtgtgga
     5701 tgtgctccgg ggcgcgctcc agctcggcga agggggcgat ggcgcgacgg catgcgtcgg
     5761 cctgctcgtg gtccacctcc aggagcagcg tcttgtcgct ctggacgatc aagcaggaca
     5821 ctcgtacctc cacaaccggg cccggccggc ccaccgcgcc aaactcccga gtctacttgc
     5881 tcgaacgtgg agccggggtg gaacgccggg gtggaacggg gcacgaccgt ttgcgcacgg
     5941 gccgtccggg ggggcgcgcc gggccgcggg ggcgggggtg gctggtcggg ccgggccgcg
     6001 gcgccggggg tcaggcgtgt tcgtccgcca gttcggccac gcctgtgatc cggtgcaggg
     6061 ggtacgtgcg gacctcgtcc gccgtgtggt cgtagccggt cacgaagccg ccctcgacgc
     6121 gcaccggggc gatgacgcgc tggctcgccg cgccctccgc gttcacgtag ccgatccaca
     6181 ccgcggcgcc cgtgagcacc gcggcctgca tcgtggcgag ggtgtccgcg gaggtggtgc
     6241 ggggcaggcc gccgtcggcc ggggcggggg cggcgaccgg cttgcgctcg gccgtggagg
     6301 ccaggtcccc cgcgcggatc gcccgcaccg cggcgcccag cagcgtctcg tcggggaccg
     6361 gcgggccctc cgggacgggg gccggggcgg tgcgctgcgg agtgcggcgg gcgtcggcgc
     6421 gggtgactac gacgtcgccc tcggccgact cggccgccgg ggcgtacccc atggcccgca
     6481 ggccctccag gagggcgtcc ggcgcgacct gcgcggcgag cacggtgggg gcgaggcggc
     6541 gcagccgcag cggggcggag cggcggtcgg cgaggatctc ggcgagcatc gcctcgtcgt
     6601 cgcagcgcag gtaggaggag gcggcgccga cgcgcagccg gccgtggcgg cgggccacgt
     6661 cgtcgatcag gtaggccagc ggctggggca cgggcgtacg ggagtgctcc gtgaggaacg
     6721 ccttcaggtc ggacgcggtc tgcccggcgt ccagcgcgcg gcgcacggac gcgggcgtga
     6781 agcggtagac cgtggcgccg cccttggact cgacgtccgc gaggaccccg agcacctggg
     6841 cgagcggccg cctcaagggg cccggggcga cggccgtcag gtcggcctgg agcaggatgt
     6901 ggtcgagtgg ctccggcagc agcggggcca gcagggcggc cgcccgtacg gcgcaggcgc
     6961 cctgctcggc gtcgctgagc gggaatgcgg gctccgtgcc ctccggtccc gccggcctct
     7021 ccggcctctc gggtccctcc ggcttttccg gccccagcag ggcgcggccc ggggcggaca
     7081 gggcgccgcg gttggtcacg cccagcagct cggcctcggc cagcgcccag cgggccaggc
     7141 gggtgcgcag gtcctccgtg ccgggctcgg caggcgtccg cggggggcgc tcccagtgca
     7201 tgcggtccag cagcgtgtcc gcggtgaccg aggcgcccgg cgggagcgtg gccaggaggg
     7261 cgagggcgcg gtggcggacc tcgggcgcgg gcgagcggtc cagctccgcg gcgaggacgt
     7321 tgaggggccg gcccttggcg tcccggctgc ccaccaggcc cggggtgcgg gtggccgcga
     7381 gccaggccgt ggccaggcgg gcccagcgct gctcggcggg caggtgcagc cagtcgtcga
     7441 aagcgggcgt gggggcgtag cgctcgtcga cctcgccgtc ggaggcgagc agccccgccg
     7501 cgtacgccag ctcgatccag aaggccgccg cctcctcggt ggtgtcgagg acggccgcgg
     7561 cgcgcttgag gtcgcgcacg ctcaggccgc ccgcgcgcag cacgagcggg ccgtcggact
     7621 cccaggcggc aagcaggtcc tccaccgtcc ccagggccgt gaacgcctgg ccggcggccg
     7681 ccgcgtccac cgtgcgggcg gagtgcggag tggcgggcgc cagctcgggt gccaccggct
     7741 cgggcgtacg gtgcgcgcgg ccgccgcgca ggtgcagggc cgcctcgcgg ggcaggacca
     7801 cgttgccggg cgaggctggc agcagcaggc cgcggtcgag cagccagcgc aggtgcgggg
     7861 cgggatcggc ggtgaccgtg ccgtagggcg ggccccacac caacctgccc aggaccgctg
     7921 tggcctccgc gggcgcaccc gccagcagct ccgccatccg ggtgcggtcc tcgaacacgg
     7981 tcgtgagcgc ggcgaccgcc gacaccgggt cgtgcgtccc cggcagcccc gccgccgcga
     8041 tgatctgctg cagccggccc ggcgacatgc ccgccgtggc ctccgccacg gtcgggccga
     8101 ggccggtggg ggaggggtgc gccgggctcg gcgccagcag ctcgcggacg gtgcggacca
     8161 gacgcagccg gtcctcgccg ccccagacca gggcctgctc gcgcaaggtg gccagggcgc
     8221 ggggcagggc ctcggcgatg cgcgggtcgc cgggggcgtc cccggtcatc agggccgcga
     8281 gagtgccgta cgggcacggg tccggggcga cggcgagggc ctcggccgcc tgcagggcga
     8341 agcggtccag gcgctccagg gcgcgcacga cggaggcgcg ggtgcccgcg cgggtcgcca
     8401 gctgggtcag atcgcccggc acgggattga ggagatcggg gcgggcccgc agcaggcccg
     8461 cgagcgcccc gtcgggccgc gcccgcaact cctcggccag cgtccgcggc ccgccgcgcg
     8521 cggccccgcc gctactgcct gtctcccgcg gtgtggtcat tctgtttacg gtaccgggtc
     8581 gagcgccgca ggccaggggg ttgcggggcg ctgcggcggt gcgggggcgg tgcgggggcg
     8641 ccggtgcgcg gggcgccgcc ggtgggtggg gggtgccggt gcgcggggcg ctgccagtgc
     8701 gtgggggtgc cggtgcgcgg ggcggcttcc ctgagccgcg tcgtcatcga ccgttcccag
     8761 cagaccgaac tcggccaggc gctcatggcc gagctgcacg tgacctacag cgacgacctc
     8821 gaagcctgac caagccaccg acgccggcgg gaacgacgac gggaggtgtc gggggcgcag
     8881 gggtgggggt tcggggcgct gacgtgtatt tgtgggccca atctccgggc acgcacgaca
     8941 tcagggggtc cgggcccgta aatatacggc cccggacccc ctgatgtcgt gcgtgcccgg
     9001 agattgggcc cacaaataca cgtcagcgcc ccgaaccccc acccctgcgc ccccgacacc
     9061 tcccgtcgtc gttcccgccg gcgtcggtgg cttggtcagg cttcgaggtc gtcgctgtag
     9121 gtcacgtgca gctcggccat gagcgcctgg ccgagttcgg tctgctggga acggtcgatg
     9181 acgacgcggc tcagggaagc cgccccgcgc acggcggaga gatcgttgcc gcgaaggtcg
     9241 agccgctggt accggccggg gttgaactcg gtgttccgca gagtgcagtc gctgaggacc
     9301 gcgtcgctca ggtcgcagtc ggtgaatgtg gcttcggaga gtgagcaacg ggagaagacg
     9361 gtcgggccga cggcgcggac cttggtgaag gcggtgaaat cgagcttgca ggattcgaac
     9421 agcacatggt cgagggtgag gccgtcgagg gccgcgccca agagcttgca atggcggaac
     9481 acgacgcggg tcagcttgct ctcggtccat cgggccgagc ccaggtcgca ggcgtcgatc
     9541 tcgaccgaat gcaggtttac ccgttccagc cgcaggcgtg ccgtgtgcag gtttgtgatg
     9601 cggccggtga tgagttgcac gtcggcgtgg tcgaggtcgc gcaagtccgc gtcggcgtag
     9661 tggaagtcct ggacactgcc gtgagccctc tccagggatt gaacgcggtg caggtacagg
     9721 cctggctcgg cgaggtcggg tacgacgatt cgggcacggc cgaagaggag gtggtccaca
     9781 ggactccgag gggtggacga gaggggtgtg ggccgccgag gagcggcccc accgccgggg
     9841 aagctacttg gtcttcttgt tccagttatc ccaggtggtc cggttgtcga actgtggtgt
     9901 gatgttgtcc caggtgggtc gattatcgaa tccgcccccc ggggcagtcg gcgacaaggc
     9961 catcagtgtt tgcagggcgt ccttcgaggc acgtacgcgg tcagtggcag tgggcgtcgg
    10021 catagcgggt gactccttcg aatagggcga tcttcgaggc ggtgcagtaa cgggtgcggg
    10081 tgccgtccag tcggccgtcg tgctcgaagt accggttcgc gggatgggcg ccgccgcaga
    10141 agtcgaagta cgggcatgtg gccttgcacg cgtcgacgcc gcgccagaac tcggcgagcc
    10201 acgacgtgcg tgcgtcagcc tcggacagca gcgtcttgag gtcggtacgc aggacgttgc
    10261 cgctggtgaa atcaccgagc cgtggatcga ggaacccggt cagctcgggg gatacgagga
    10321 ccaccccacc gtcgtacgcg atcgtgggaa gcgggtccca cgaccggccg ccccgggaga
    10381 ttgctccctg gtcgttcagc acggcccggg cgaagtccag gacccggctg atctcccgca
    10441 ctcggacggc ggggttggtc tgccacgccg cggtcagcgc ttcccagaac tcggccgcct
    10501 gctccgtctc atggtgagcg gtgttcacgc cctcgtgttc ttcgatgttg acgccgagcg
    10561 agcggcagcc gaggccggcg aagaacgtat agaagtccgc tgcctgtgca gggtcgggat
    10621 cggagacgac agcgatcaca tcgaacggga tttcgtgacg ccgaagcctt tcgataccgc
    10681 gcatgatcac ctggaatgcc ggatgaccgg cacgggtgat gcggtgggag ttcatctccg
    10741 caggaccgtc gatgctgacc cctacgccga tgtccttctc cagaaggaag gcgcaccagg
    10801 cgtcgtcgag gagcgcggcg ttcgtctgca cggtgtgctt cacgccgcgg aacggggcca
    10861 tcagggccgc caggtgctcc cggccggcgg agagcggttc gcctccgtgc cacaccacct
    10921 cgaatgacga atcttccttc gcccaggcgt tgaccgacgt ggcgaccgct gcggccactg
    10981 ccagtggcat gaggtgcttg gccttgcgga agggcagata gcagtatgtg cagtccaggg
    11041 ggcggcacag tgtcgtcggc tgcatgacga cagcctctgg taccgcggca aggcgcctcg
    11101 tcggtgctgc cggagtggga gcggacacgc gtatcaccac cgattcgtgg ggcggtcagg
    11161 ctgcaaacag catctgtagg cggaccggct gtgctcaaga aacttgcacg cgcttgcagc
    11221 ggttcattcc aaggcgttga tcgctgcggc gatcaacgtg cgggcttcct tgccgtagca
    11281 ggcgatctgc gcgagttcgg agaacatttg tgcgtagatg gcgacttcgc tcggttgctt
    11341 cacggtgacc tgggcggtgg ccagctcgat gatcacctgg tcgtcatcga agatccagaa
    11401 gccttccagt ggccacatcg tgcggtcggc cgtcatgggg acgattccga gactgacctg
    11461 ttggagcacg gatacggtca gtaggtggcc gagctggctg accatgacct ccgcgtcacc
    11521 gaagcgggtt cgcagtgccg cttcttccag caggatgccg aactgccgat tggcctggcg
    11581 caggacccgc tgccgcccca tgcggacagc gacggcggcc tcggtgtcgt cagggatgtc
    11641 ccagaaagca atgatgcggc ccatgatcga ccgggcgtag gccggcgttt gcagcaggcc
    11701 ggggatgacg cccggctcgt agatacggaa acgtcgggtg cgctcgtaca gcggaacgtg
    11761 cgcctcttgc acagggcgca gcccttcacg ctggaggcgg cgccactcga cgtacatctc
    11821 ctcgatgttg tgggcggtgg cgaggagatc ggggtacgtg tcggtggcct gacacagttc
    11881 ggcgtatcgc cacagatcgt catcggacgg ttgcgtgatg ccgttctcga tgcgggaggc
    11941 cttggactca ctccagccgg cacgggctgc gaaagcccgt gccgttttga agccgccagc
    12001 tttacggatc tcccgaagcc ggctgcccag agcgacgcgg gcctcccgga cagcggaaga
    12061 gggcgctgat ctcacaggtc tgcttcgttg tcgtcgctca ggagggccgg tattccgcgt
    12121 gcgggaccgc ccgctcccag actgcctcga acgcactcgt gcaaaggcgg aggacccgct
    12181 cgtcccgcgt gggttccagc cagtccggcg ccgcctcccc gtcacccgtg aagtgcagga
    12241 ccacgaccag ctggttgtcg aatacccaga agtcgttgcc cgggaaaggg atgtcggtcg
    12301 cctgcctgcg cggcagccag cggatgtcct cgcccgcttt cgcgttggcc ttcgagacgt
    12361 agtgctcgaa gcggatgcag tccgactccg gtacggagaa aacccgcgcc cggcggatgc
    12421 cgacgccgcg gccggtggca gcccacaccg tctccagcca ctgctggaat tcgacgtcgc
    12481 cacccaccgg atcggtcagg gtcccgttct gccaggcgat gaaggcgggg tcggacggca
    12541 tgtacgtgtc gcgcatctcc agatgcaccg ccgagtgctc ggccgaggcc agaagctcct
    12601 caatcgtcgg cacccttgcc accctgcacc tccaggaaga actggaccat ccgccgggga
    12661 atctccacga ccgtctcgtg cctcggaaga tcgagctgcg ccagccgctc catgtcgtcg
    12721 accttccacc cctggacaag gtaggtgtcc ttcttctcgt ccaggtaaat cgtcggtgat
    12781 ccgcccgact tgctctctgg atccttgcca agcctgcgca gagccatggt tccctcctag
    12841 ctcgggggcg atctccggag accagccttg cacctgccgc aggagagacg cacggaactt
    12901 gcgccgactt gcatgtggac cggcagcctg agtggtcaca gccggccgga ccggacctcg
    12961 tggaggaatg aggtccaggc cgctggctgc ccatggagcg gcacgtcatc cacctcttca
    13021 gcaccggccc gtggccagca ggtgtgaact ggcagcgagt agatccgggt acggctccgc
    13081 ggctcgggcg gcggccaggg cggcccggaa gagcggcgag tcgaggggca acggttgttg
    13141 ggtgtgctgc tcggcggcct tgagtaggga ccacgtgggg ccttcgacgg cgtagacacg
    13201 gatgtcgtgg agtccggcgg cgcgcatttc ctccgccaat tccggggcgg tgtggaaata
    13261 ggccgctgtg aagcccttcc tgcccggctc gtgcacgccc gtggcgagga tgtcggttac
    13321 ggcgccgcgc acgctctcgc ggtccagcca cgtggtggcc gtgtgctcga agagggatgc
    13381 gtaccggttg atggccgcgg cggccaccag gccaccggat gcggtgacgc gacgtgcctc
    13441 ggccagcgcc tggtcgcggt cggcgcggtc gagcaggtgg tagagcgggc cgaggaggag
    13501 cgtgacgtcg tacgacccgt cggcggcggt gaggtggcgc gcgtcgccga gttcggccgt
    13561 gcatccggtt ccggcggcct gtgtcacgtg cctggccacc gggtccacga ggtggacccg
    13621 gtaaccgtcc gcggtgagcc accgcgcgtg ggctcccggc ccgccgccca cgtcgagaag
    13681 tgcggccggg gcgggaggga gatggcgccg gagcagctct tgtgtgcggg ccatctccag
    13741 catgccgtcc gctgttgccg tcagccgggt ggcctcgtcg aaggtgtcgg tgtagaagtc
    13801 gatgatctcc ggggccactt gggggatcgc catggcggcg atgttctacc gtgaccggtc
    13861 gacatgtcca gcggtcacag ccggccggac cggacctcgt ggaggaacga ggtccaggcc
    13921 gatgggtgta cgtggagtga cgggatgctc gtgtccttgg agtcgcggat gccgatcccc
    13981 tcggtgaggg cggcgacctc gacgcagttg tgttgcgcat tgctgtagga ggacttcatc
    14041 catatgtcat gcatcgctga cctctcctga tagttgttgc agaaaacgca cggtcttctc
    14101 cggcgacatc gccgtggcgc tgagggagct gaaccttcgg gagaagcgtc cgacctctcg
    14161 tggcttgtcc gacatgcaca gggaccccca gggcacgtcg gcctggaccg tcgacggcaa
    14221 tgcctcgccc aggtcgagga tggtgaagtc cgtgtcgaac cggtagccga cctcgtgggt
    14281 gcgcaggatc tgcactgtca cattttcccg ctccgacagc gcggcaatct ctgcatactg
    14341 ctcgcgcatg acctcggcgt ttccgacgat gtggcgtagc gccgcctccc ccaggatcgc
    14401 ccagaggtgc aggggatcct cctcgcgggt caggagttcc ttgcgcatca tgcgcaggcg
    14461 aacgccgtgg tcgacgtact cgctcgtgaa ctcgtcgtgc aacttctcca tctcgaaaat
    14521 ggcggttgcg tacctcttcg tctggagcag gcccaggacc agggtcgggt gccatgcctt
    14581 gatggcgcgg gctacggatt cgacgcctgc gaacgtccgc accgtggggt gcaccgtgct
    14641 ggcgaaggac gtccattcct ggcttgcgga ctcgcggtgc agctgggtga gcgcagcgat
    14701 gtggtcctcg tctgtgacgt cgtaccgctt cagcagactg cgcaggtgac tggattgctt
    14761 gagcggaatg ctccccgtct cgacgcgctg gaggtgggac tcgctgaagc ccaggccctt
    14821 caccgcctgt tcgagtgtca tcccggcttc gctgcgcatg cggcgtagtt cggtgccgag
    14881 tgcgatacga cgagctgttg gtccgatccg tgctcccact ctcatcgcct tccgtggtcg
    14941 cctgcgtcct ggctgagtct ggcaccgcgt gttcatgccg cgcttctcgc tgctcctctt
    15001 gtgtgcgcgc ctgtcgccgt caggcatcgc tcaagtcccg ccagccatgc acgtgttgcc
    15061 gtcctggcat gtgccacttc ggaggctagc gggatatctg tgggatggcg cttgaatcct
    15121 caattcgcca cccggcaggg tggttcccgt accgagtcgc aggtcggggc gatgcccccg
    15181 caccgctcga aggagccgtc atgcctccct tcccccgtac cgcccacacc cccggcgaga
    15241 tcacgctcgc cccctccgag ccgtcccccg tttcgatcgg tcaggccctc cgtgaggcca
    15301 ccgccatgga gacgtggttg cctgccccgt cgcggctgag cgaggtcacc gagcggcttc
    15361 tcgggtacat ccgggagacc gtcccggccg tggaggcggc tgcgggccgt cgccccgtgg
    15421 agtgtccggt ccgccggtcc gcgttcggcg cggtggagga agcccgtcac cgggccgggg
    15481 gcgggcccgg cgacgggtat gcgtccgcga tcagcttcgc ccggggcctg ggcaaggctg
    15541 cgggggacct gatgcaccag cagcggcggc tgcaacggga aggcgagcgg tgaagggggc
    15601 ggctgcgcgg ctgtgggcgt ggtggggtct gtacgggcag ttctcccgtc ccgtaggggc
    15661 cttgtggccg agccgccgac ggacgtatgc caagcggcgc aacgaggtgg tggccgtccc
    15721 ccacaccggt ggcccgcccg cgctcaagca actgccgggc tgcacctgtg aagcctttgt
    15781 cggcggggaa cagagggcgt aggccggtcg ttcaggtctc gcacggtcca ggcaggcatg
    15841 cccgttgcgg actttggaga gtgctcgatg cccaagtggt tggtcatgga ggagacggtc
    15901 gtggccgagg gagcggctcc gcatcagcgg atcctgcggt ccttgagcgg gaacaagacc
    15961 cgggatcagg cgctcgtgga gttgagcaga gtggccaagc gccaccggcc cgactgcctc
    16021 aagagcacgc cgtgcgtcgt ggggcaggac gacgacgggt ccttttggat tctgccgaag
    16081 aacggcaaag ggcacgcccg gtgcaacctc cgggtcattg agcagacgca cccgtaagtg
    16141 acggtcgtga gcgggatggg gcggtcggtc cctgtcttcc tcggggccgc cccatcccgc
    16201 tcggccgtca gtcgaaccag cgcgcccgcg ccagttcctc cgtgcggctc gggtcctcca
    16261 ggagggccgc tgcctcgaag cggcgggacc aggctcctgc cgcccaggcc aggcccgccg
    16321 ccacgccctc cagcgtcgag gcgtgcagga tgccgtccgc tcccaggcgc cagtccagtt
    16381 cgacgccgtc gacgacgagt tcctcgtgct ccacgtacgt ctccggcgcg cccggcagca
    16441 ggacgcgtac gccttccggg acctcgcgga gttcgccctg gcccgccggg gcggatgctt
    16501 ccagggtctc gctcaggcgg cgtacctgga gcaggtcggc caggtccgcc gagcgtgacg
    16561 gcgtcaccgg gaggagggcg tgacccgtgg ccaaggggag gaggtccggg gcgtcggcca
    16621 ctaccgcgtc cgccgcgtcc accacccgca cctcgccgtc ggtcacggcc cgcagctcgt
    16681 ccggcagcgt gacctggtcc gggtcgaggc gggcgaggag gccgtacagg gcgtggagct
    16741 ggggtgcggt caccgggagg gccgggtcgg ccaggcggga gaggagttcc gccgcgccgc
    16801 ccggttcgtc cagcagcgcg gccgcggacg tacggacgcc gagggcgcgc agcacctgct
    16861 cgtcggcgag gccggccgcg tccaccgcct cgtacaggcc gcgcaggagc gggtcgccgc
    16921 ccgcgacgcg cagacctgcg gggcggcggc cgtcgaggac ggggtggccg cgcagccacc
    16981 aggcggtgta cgggcggacg gactccgtcg tgccgtcggg cagcaggatg cgcacgggcg
    17041 tggtgagggc gtcgcgcagc ggcggctcgg cgagcagggc cagggcttcc ggccaggcgt
    17101 cgtcgtcgac gaggtcgagg tcgcggacgg ccacgatctc cgtcgcgacg ggcggcacgg
    17161 gcgtgtcggg cagccggtcg aggacgtcct cgcaccacac gtcgaccgcg tcgagcagcc
    17221 cggcatcgtc gggctcggcg aagtcgccgt cgcgcggttc gagttcgtcg gggtcgagga
    17281 cgacgtccgt ggcgcgcacg agcgcgaagc cggccatgac gccgacggcg gtgagcggct
    17341 gctcgcccca ccgctcggcg agggcggcgt cgcacgcggc gagctcgccg ggccggatca
    17401 cgcgttcgaa tacgcttccg ggcaggacga gttcgcccgc gggggcgagc tcgccgtcct
    17461 cgtcgggcag ggcgagggcg ccgagccagg gctcgtcgcc gggcgcgagg tcggcgtcgc
    17521 gcacgaggcc gaggacgagc tcggcgaggg cgtccgcgtc gagcgcgtcc tcgtcccaca
    17581 cctcgtcggc gtcgagggag gcggccaccg cggcccgcac ctgcggggtg gtcaggacgg
    17641 cccggggaga ggacggggtg gcgccgagct tctccaggag cgggtgcgcg gcgtcggggt
    17701 gggcgacctt cagccccagg cgggagagcc gctccgagtc cgcgccgtcc ggcggcggga
    17761 gcagcacctg ccgcgggccg atggccgtac ggccgtccgc gagcggcacg ggcaggccgg
    17821 tgagccggtc cgggtcgatg ccggcgaggc tgtcgtagag ccgctgccac caggacgggg
    17881 cgcgctcgac gccggcgagc cggtcgacga cctcgccgag cggggcgcgc gcgacgccga
    17941 gggcgcgcag ctcggggcgg cgctccaggc cggcgggcag caggctcggg aagagctcgc
    18001 cgaggacggc gacggtctcg gcgcccgcac cctcgacgac ctcggcgtcg gcggggcgca
    18061 gcgcgtacgg ctcgccgggg ccgccgggcc cgctccgcgc gaacgggtcg tcccacgtgt
    18121 cggcggccgg cggggcgtcc ggggcggcgg ccggctccgg cgtctgccgg gccgcgtccg
    18181 ggccctcggt ccacgggatg ccctccacgc cgccgccccg cggcgcggcc gtctcctggg
    18241 cggggacccc ggccgcgctc ggcaggaacg cgacgcccgg cagccggtcc aggatccgcc
    18301 ggcgcagctc cgcgtccagc gcgccctgcg ccagcgggga gggcacgagg tcgagcgttc
    18361 ccgggccgac cgggtgccag tcgcgcagca gctgcgcgta cgcctcggcc gcgcgcccgg
    18421 tgaggaagtc cgtgagggcg ccgggcgcgg cgtggcggcg cgtgggctcc agcgggaacg
    18481 tggcgacgag cagcgccggg acgcccagcg gctcgtccgt cggcgtcggc gcgtgcacga
    18541 cgggcgcggt gccggggcgt acgggcgcgc cgtcctcgcc caccggcacg gcccacatga
    18601 ccgaccagtg cgggcgcagg cgctcctcca cgggccggtc ggcgagcagc gcggagtcca
    18661 gggccccggc cgcccgctcg acccgccagc gggccgtggt gccgcgcgcg gagtcgtcca
    18721 cgaggacgta cgggccctcc tggcggcgcg tcagggtccg tacgacgccg tccgtctcga
    18781 cgacgacctc ggacaggccg ggcagcgtca gcagcagcgc gtcgtcgacg ccggccagca
    18841 gacgggaggt caggtcctcg gcggcgccgt cgcgcagggg cagcacgacc accgtgtcgt
    18901 agccggccgg ggcggagccc tcggcgggca gcgggagcct cagcagcggc acgtggccct
    18961 cgcggcggcg gatctcctcc tccagggcgg gggcccggac ggcctcgcgc gccgccagcg
    19021 ccctcgcctc cgcgagcgac cagcggacgc cgccggagcg gccgacgacg gccggctcgt
    19081 cgctgacggc caggaccgcg gagaagccga cgccgaaccg gccgacgctg ccgtggccgc
    19141 ccggcccggc gcccttgtcc gcgtcgcgct tggcggaggc gcggagggtg gccagggact
    19201 cgacgcccgc ggcgtccatg ggcgccccgg tgttcgccgc ggccagcacg gccgggcggc
    19261 tgccggcggc cgcgtgcagg gtcagccgca ggcggccggg gacgcccgcg cgggccgcgg
    19321 cgtcggcggc gttctgggcg agctcgacga cgaggcggtc gcggtagccg ccgagcgcga
    19381 ggtcctcctc ggcgttggcg tcctcccgga aacgggccgg cgacgccgcc caggcgtcca
    19441 gcacgccgcg ccgcaggcgc gcggttccga acggatccgc gccttcggcc gcgcctcgca
    19501 ccattgccgt gccgctcacg tgtcgtctcc tgccgccggt gggtgttcct acggcagaac
    19561 gtaccgcgcg ggggtgacag gccggtgtcg ccctacccgt gggcggccgt acgacagggc
    19621 ggggccgtat cgcgcccggg gggcgctcgt cctcaaactc ccccagctac tgctgggagg
    19681 tacccccgga cgggctggat cggcgtcggg ctcgcccgtc aggggcagcc cccgtcagga
    19741 ggccggaggc tccggcccgt ccgttcgcag gccgagtgcg caggccgagt gcgcaggccg
    19801 agcgtacgtc agcccgtctg cggaggcgcc gggcgcgagc ccccggggtg cctcaggagt
    19861 gcccgagctc ctcgtcctcc tcgtcgccgg ccggctcgga acgcagcggc agcgggtcga
    19921 cgagcgtctc gtcgatcacc ggcggcgccg gccgcggcgg cttcggcatg accgcggcct
    19981 cggagtgccc gccgcagccg tacgacaggg acacgaggtg cccgtccgcc ggaccgaact
    20041 cgttggcgca gacgccgaac gcctgtccga gggagccccc gatcggcacg aggaagccgc
    20101 agctgacgca cgacgccggg gcggcctgcg ccatcggggt cttcgcgccg tgggtctcgt
    20161 cccagcggtc ggcggcggtg tgcagcccgt agcgggacag gacgcgggcg cgccgcatcc
    20221 cgagctcctc ggccacggcc gcgatcgtgc cgcgcgccgg gacggccggc tgggcggcgg
    20281 gggagccggg gacgacgtcg gcgtcctcgg cctcggcgag gcggtccatc tcctcggaga
    20341 cggcggagtt cggcggcggg acgtcctcgc cgctccagcc gggcacgagc cgcaggtcct
    20401 cggcgtcggt ggggaggagg tcgcccgggc ccatgtcgcc ggggcgcagg cgctcgctcc
    20461 aggggaccca ctcgggggcc atgagggcgt cgggcccggg cagcaggacc gtctcgtcga
    20521 gggtgacgtt cttggcgcgg gaggcgcggg cgacggtgac ggcccagcgc cagccgcggt
    20581 aggccgggtc caggcagagg aaggagtggg tgacgacgcg gtccccgtcg gctacggcct
    20641 ggacatgctc gccgaccgtg ccgggcagcg cggcctcctc ggccgcagca cgggccaggt
    20701 cgaccgcctc ggcgcacagg cggtccgggg tacggcttcg catcgcagca ctcacaagaa
    20761 tcgattctct cttccacgcc gtctcacgag tgcgccagcc gatcggccgg gtcggcggac
    20821 ggagcggacc tcaggaccgc gtcgacgtcc gcgcccgaac ggcctcgggc acacctagtc
    20881 actcccattc tgcgggatcg agactcggag cgcggccaag aacgaccgcc ggtggcgcgc
    20941 tacgcacgct accccgctcg gcgccaccgg ggatagccgg gccgcggcga gccccgcgct
    21001 cgcccggccg cgagggggcc gcgccaggcc ccgtgcggcc cgcagcggcg gcgaaagtgc
    21061 cctgcccggt gcgagttggg gcagtatggc gtggtgggag ccatccggtc ggtcgagggc
    21121 ggtccgctgc gccgcatggc
//
