LOCUS       NZ_LNBE01000004        10399 bp    DNA     linear   CON 29-MAR-2024
DEFINITION  Streptomyces roseifaciens strain MBT76 scaffold_4, whole genome
            shotgun sequence.
ACCESSION   NZ_LNBE01000004
VERSION     NZ_LNBE01000004.1
KEYWORDS    WGS; RefSeq.
SOURCE      Streptomyces roseifaciens
  ORGANISM  Streptomyces roseifaciens
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1  (bases 1 to 3642602)
  AUTHORS   Wu,C., Zhu,H., van Wezel,G.P. and Choi,Y.H.
  TITLE     Metabolomics-guided analysis of isocoumarin production by
            Streptomyces species MBT76 and biotransformation of flavonoids and
            phenylpropanoids
  JOURNAL   Metabolomics 12, 90 (2016)
   PUBMED   27073352
REFERENCE   2  (bases 1 to 3642602)
  AUTHORS   Wu,C., Zhu,H., van Wezel,G.P. and Choi,Y.H.
  TITLE     Metabolomics-guided analysis of isocoumarin production by
            Streptomyces species MBT76 and biotransformation of flavonoids and
            phenylpropanoids
  JOURNAL   Metabolomics 12, 90 (2016)
   PUBMED   27073352
REFERENCE   3  (bases 1 to 3642602)
  AUTHORS   Wu,C., Du,C., Choi,Y.H. and Van Wezel,G.P.
  TITLE     The complementarity of genome-mining and NMR metabolic-profiling
            facilitates lead-finding
  JOURNAL   Unpublished
REFERENCE   4  (bases 1 to 3642602)
  AUTHORS   Wu,C., Du,C., Choi,Y.H. and Van Wezel,G.P.
  TITLE     The complementarity of genome-mining and NMR metabolic-profiling
            facilitates lead-finding
  JOURNAL   Unpublished
REFERENCE   5  (bases 1 to 3642602)
  AUTHORS   Wu,C., Du,C., Choi,Y.H. and Van Wezel,G.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2015) Molecular Biotechnology, Institute of
            Biology Leiden, Sylviusweg 72, Leiden 2333BE, Netherlands
REFERENCE   6  (bases 1 to 3642602)
  AUTHORS   Wu,C., Du,C., Choi,Y.H. and Van Wezel,G.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-NOV-2015) Molecular Biotechnology, Institute of
            Biology Leiden, Sylviusweg 72, Leiden 2333BE, Netherlands
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Velvet v. 1.2.06
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 200.0x
            Sequencing Technology             :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_001445655.1-RS_2024_03_28
            Annotation Date                   :: 03/28/2024 03:28:28
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,528
            CDSs (total)                      :: 7,450
            Genes (coding)                    :: 7,245
            CDSs (with protein)               :: 7,245
            Genes (RNA)                       :: 78
            rRNAs                             :: 2, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 3, 2 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 205
            CDSs (without protein)            :: 205
            Pseudo Genes (ambiguous residues) :: 0 of 205
            Pseudo Genes (frameshifted)       :: 77 of 205
            Pseudo Genes (incomplete)         :: 168 of 205
            Pseudo Genes (internal stop)      :: 20 of 205
            Pseudo Genes (multiple problems)  :: 56 of 205
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 13:37:43
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 1044746
            Orig. end                         :: 1055145
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            LNBE01000004.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10399
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="ectoine"
                     /region_number="3"
                     /rules="ectoine_synt"
                     /tool="antismash"
     cand_cluster    1..10399
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="ectoine_synt"
                     /kind="single"
                     /product="ectoine"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10399
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5399](-)"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5399)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
     gene            complement(312..1595)
                     /locus_tag="AS857_RS39115"
     CDS             complement(312..1595)
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA]"
                     /GO_function="GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /GO_process="GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1048: sensor
                     histidine kinase"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004002232.1"
                     /locus_tag="AS857_RS39115"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="histidine kinase"
                     /protein_id="WP_058044963.1"
                     /transl_table=11
                     /translation="MTALLRRGAVALVGRRARLRWVHLLLGGALLMPYYLLASTLTAVF
                     VRDTNILFPSTVGRQFGVFALALVPAAVTALVPLVRTLESVAARALCGVPEEALATGPG
                     DSRAARGRTALWYTLHLLAGGIVSGMSLATPPFALTLIALPVTGVPGGEDAPYGLGDLA
                     FPWTLLAVPAGLAVLVLLVGASWGAGTLLARCAPLLLGPTAADRLAEAEQHAMRLALRN
                     RLARELHDSVGHALSAVTLQASAARKVLDADPEFARQALAAIEETTREAVGELDTVLGL
                     LREDGAASAAPAPTLADLDGLLERTRAAGGTVTLASPPALDGVPPVVSREAYRIVQEGL
                     TNALRHAGRVPVLLRITVHDDAVEVALENPVNPDKAPGRAHRRTGGGRGLTGIAERARL
                     LRGSSSSGERDGVWRLAVRLPLGGGAGR"
     gene            complement(1592..2599)
                     /locus_tag="AS857_RS39120"
     CDS             complement(1592..2599)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004002233.1"
                     /locus_tag="AS857_RS39120"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_058044964.1"
                     /transl_table=11
                     /translation="MTSTTTTVPDDHATKRRALRAVTIAACLPYLGLKAAWVAGSHIGI
                     PEGSGLRDADTSMVVANAVTILMDAAVVVLALLLTRPWGKRVPAWLLVLPMWGAVGLLA
                     PIMYAFPAQLIVRALGGSASGSGSEADEPFLDEWVFGVVYTGFIVQGLALGTLFVLYAR
                     ERWGHLWRGRVGDLAGRPPGRGQRAALAAAGVLAVLPGAVHLMWTCGVSAGLSPEMARM
                     YSGTFAAGESGYVLYAAATIAGAVLLLSGRARSGHGGRLPLRVPLLLAGVGSAASAAWG
                     GWQLLAMLTSLADEGRGPTATMAVAYAVQVIIGLLVLAAGTRFFVQRSARAAAL"
     misc_feature    2590..2608
                     /note="TFBS match to LexA_variant_3, Repressor of DNA
                     damage response, confidence: weak, score: 19.55"
                     /tool="antismash"
     misc_feature    complement(2591..2609)
                     /note="TFBS match to LexA_variant_3, Repressor of DNA
                     damage response, confidence: weak, score: 18.4"
                     /tool="antismash"
     gene            2881..3921
                     /locus_tag="AS857_RS21785"
     CDS             2881..3921
                     /NRPS_PKS="Domain: Aminotran_5 (13-340). E-value: 2e-26.
                     Score: 84.7. Matches aSDomain:
                     nrpspksdomains_AS857_RS21785_Aminotran_5.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_5"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1139: aminotransferase class V"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014180697.1"
                     /locus_tag="AS857_RS21785"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="WP_058044965.1"
                     /sec_met_domain="Aminotran_5 (E-value: 5.8e-20, bitscore:
                     69.1, seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MESLAPAHFAPETVYLNTAATGLVPSPAVAAMTEAATAFSLGRPG
                     AEDHFPVVEAVRATFARLVGVPARQVATGGSAAVFCGLVAQSLPEESEVLCAEGDFSSL
                     VNPFHVRPGLRVRTVPLEKIADAVGPGTALVAVSAVQSADGRLAGLGAIGEAARAHGAR
                     TLVDVSQAAGWLPVDAGAHDFTVCVGYKWLMGPRGTAYLTVPEDLGGLTPVFAGWVAGE
                     DPWDSTYGQVSLARSARRYDENPALLPYTGALAGLRLVEELGTERIAAHDRALADRFRT
                     GLAALGHRSAPAEDSAIVSVPGLGRAAERLAAAGVRVSARAGGLRASFHLYNSTDDVDR
                     CLEALA"
     aSDomain        2920..3900
                     /aSDomain="Aminotran_5"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_AS857_RS21785_Aminotran_5.1"
                     /evalue="2.00E-26"
                     /label="AS857_RS21785_Aminotran_5.1"
                     /locus_tag="AS857_RS21785"
                     /protein_end="340"
                     /protein_start="13"
                     /score="84.7"
                     /tool="antismash"
                     /translation="VYLNTAATGLVPSPAVAAMTEAATAFSLGRPGAEDHFPVVEAVRA
                     TFARLVGVPARQVATGGSAAVFCGLVAQSLPEESEVLCAEGDFSSLVNPFHVRPGLRVR
                     TVPLEKIADAVGPGTALVAVSAVQSADGRLAGLGAIGEAARAHGARTLVDVSQAAGWLP
                     VDAGAHDFTVCVGYKWLMGPRGTAYLTVPEDLGGLTPVFAGWVAGEDPWDSTYGQVSLA
                     RSARRYDENPALLPYTGALAGLRLVEELGTERIAAHDRALADRFRTGLAALGHRSAPAE
                     DSAIVSVPGLGRAAERLAAAGVRVSARAGGLRASFHLYNSTDDVDR"
     gene            complement(4080..4997)
                     /gene="thpD"
                     /locus_tag="AS857_RS21790"
     CDS             complement(4080..4997)
                     /EC_number="1.14.11.55"
                     /codon_start=1
                     /gene="thpD"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PhyH"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016436210.1"
                     /locus_tag="AS857_RS21790"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="ectoine hydroxylase"
                     /protein_id="WP_058044966.1"
                     /sec_met_domain="PhyH (E-value: 1.3e-35, bitscore: 121.1,
                     seeds: 39, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MAAVTDLYPTRGTHEVAVPRRDPVVWPGSGSASGAGTGTGTPGPI
                     DAAGLAGYERDGFLTADEMITPDEVQVLRAELDRLIADPAVRADERAVVEPHSKDVRSV
                     FEVHRISEVFARLVRDPRVVGRARQILGSDVYVHQSRVNVKPGFGASGFYWHSDFETWH
                     AEDGLPRMRAVSVSIALTENLDTNGSLMIMPGSHRTFLGCAGVTPRDNYKRSLRMQEAG
                     TPSDSALAALAGRHGIRLFTGAAGSATWFDCNCMHGSGDNITPFPRSNVFIVFNSVENT
                     AVEPFAAPRRRPEFVGARDFTPVK"
     gene            complement(5001..5399)
                     /locus_tag="AS857_RS21795"
     CDS             complement(5001..5399)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     ectoine: ectoine_synt"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006123624.1"
                     /locus_tag="AS857_RS21795"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="ectoine synthase"
                     /protein_id="WP_058044967.1"
                     /sec_met_domain="ectoine_synt (E-value: 4.6e-54, bitscore:
                     179.2, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIVRSLQDIENTDRHVKAKSGTWESKRIVLAKERVGFSLHETVLY
                     AGTETSMWYANHIEAVLCVEGEAELTDDETGEKHWISPGTMYLLDGHERHTLRPKTDFR
                     CVCVFNPPVTGREDHDENGVYPLLTEEG"
     misc_feature    5403..5419
                     /note="TFBS match to HexR, Glucose-responsive regulator,
                     confidence: weak, score: 15.37"
                     /tool="antismash"
     gene            complement(5459..6727)
                     /gene="ectB"
                     /locus_tag="AS857_RS21800"
     CDS             complement(5459..6727)
                     /EC_number="2.6.1.76"
                     /GO_function="GO:0045303 - diaminobutyrate-2-oxoglutarate
                     transaminase activity [Evidence IEA]"
                     /NRPS_PKS="Domain: Aminotran_3 (28-365). E-value: 3.4e-73.
                     Score: 238.5. Matches aSDomain:
                     nrpspksdomains_AS857_RS21800_Aminotran_3.1"
                     /codon_start=1
                     /gene="ectB"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_3"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1013: aminotransferase class-III"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014156968.1"
                     /locus_tag="AS857_RS21800"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="diaminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="WP_058044968.1"
                     /sec_met_domain="Aminotran_3 (E-value: 1.1e-75, bitscore:
                     252.4, seeds: 13, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTITPPALSVFETLESEVRSYCRSWPAVFDRAQGSRMYDEDGHTY
                     LDFFAGAGALNYGHNNPVLKRALIDYIERDGVTHGLDMGTTAKRAFLEAFQDVVLRPRD
                     LPYKVMFPGPTGTNAVEAALKLARKVKGREAIVSFTNAFHGMSLGSLAVTGNAFKRAGA
                     GIPLVHGTPMPFDHYLDDQVPDFLWFERLLADQGSGLNTPAAVIVETVQGEGGINVARP
                     EWLRGLAALCERHDMLLIVDDIQMGCGRTGAFFSFEEAGITPDIVTLSKSIGGYGLPMS
                     LTLFRPELDVWEPGEHNGTFRGNNPSFVTAAAALDAYWTDGQMEKQTLARGEQAEEALR
                     AICAEHPELGAHYRGRGLVWGLEFADKPRASAVARRAFELGLLVETSGPEGEVLKLLPA
                     LTISPDELDEGLRIVARAVRETA"
     aSDomain        complement(5633..6643)
                     /aSDomain="Aminotran_3"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_AS857_RS21800_Aminotran_3.1"
                     /evalue="3.40E-73"
                     /label="AS857_RS21800_Aminotran_3.1"
                     /locus_tag="AS857_RS21800"
                     /protein_end="365"
                     /protein_start="28"
                     /score="238.5"
                     /tool="antismash"
                     /translation="FDRAQGSRMYDEDGHTYLDFFAGAGALNYGHNNPVLKRALIDYIE
                     RDGVTHGLDMGTTAKRAFLEAFQDVVLRPRDLPYKVMFPGPTGTNAVEAALKLARKVKG
                     REAIVSFTNAFHGMSLGSLAVTGNAFKRAGAGIPLVHGTPMPFDHYLDDQVPDFLWFER
                     LLADQGSGLNTPAAVIVETVQGEGGINVARPEWLRGLAALCERHDMLLIVDDIQMGCGR
                     TGAFFSFEEAGITPDIVTLSKSIGGYGLPMSLTLFRPELDVWEPGEHNGTFRGNNPSFV
                     TAAAALDAYWTDGQMEKQTLARGEQAEEALRAICAEHPELGAHYRGRGLVWGLEFA"
     gene            complement(6844..7413)
                     /gene="ectA"
                     /locus_tag="AS857_RS21805"
     CDS             complement(6844..7413)
                     /EC_number="2.3.1.178"
                     /GO_function="GO:0033816 - diaminobutyrate
                     acetyltransferase activity [Evidence IEA]"
                     /GO_process="GO:0019491 - ectoine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="ectA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010987798.1"
                     /locus_tag="AS857_RS21805"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="diaminobutyrate acetyltransferase"
                     /protein_id="WP_079110555.1"
                     /transl_table=11
                     /translation="MTAAQADLARARPASLREFPELPEDLTLDVPRVEDGAAIWRIARD
                     SGTLDLNSSYSYLLWCRDFAATSAVARRTGDGEPAGFITGYVRPARPRTLVVWQVAVDR
                     PHRGRGLAAALLEGLVARVARESETGIDGIETTVTPGNTASNRLFASFAERHGAPVERE
                     VLFHGGLFPETGHDPEVLHRIGPLTH"
     misc_feature    complement(7570..7585)
                     /note="TFBS match to CRP, Cyclic AMP receptor protein,
                     confidence: weak, score: 16.03"
                     /tool="antismash"
     misc_feature    complement(7604..7615)
                     /note="TFBS match to CcpA, Catabolite control protein A,
                     confidence: weak, score: 15.74"
                     /tool="antismash"
     misc_feature    complement(7689..7703)
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 14.62"
                     /tool="antismash"
     gene            7836..8936
                     /locus_tag="AS857_RS21810"
     CDS             7836..8936
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Amidohydro_1"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003960017.1"
                     /locus_tag="AS857_RS21810"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="amidohydrolase family protein"
                     /protein_id="WP_063278427.1"
                     /sec_met_domain="Amidohydro_1 (E-value: 8.6e-16, bitscore:
                     55.6, seeds: 57, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSDSGVLHIKGRVLVGADPDGGVRDELWAVDGRITFERPVGARDV
                     TTVEGWALPGLVDAHCHVGLDAHGPVDESTAEKQALTDRDAGALLLRDAGSPSDTRWID
                     DREDLPRIIRAGRHIARTRRYIRNYAHEIEPDDLVAYVAREARRGDGWVKLVGDWIDRE
                     TGDLSSCWPRGEVAAAIAEAHRLGARVTAHCFAEESLAPLVEAGIDCIEHATGLTEETI
                     PLFAERGVAIVPTLVNIATFPRLADGGEGKFPRWADHMRRLHARRYETVRAAYDAGIPV
                     FVGTDAGGSLAHGLVGQEVAELVKAGLPPLAALSATTWSARSWLGRPGLEEGAPADIVV
                     YETDPRTDVRVLGSPKRVILRGRVIR"
     gene            complement(9431..10381)
                     /locus_tag="AS857_RS21815"
     CDS             complement(9431..10381)
                     /GO_function="GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003986841.1"
                     /locus_tag="AS857_RS21815"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="WP_058044971.1"
                     /transl_table=11
                     /translation="MSTPRIAPTAADRTARLAVTVFPLLVLAAGGVGLAAPSHFTGWAP
                     AVPWLLGVVMFTMGLTLTGDDFRAVAKRPWAVGLGLVAHYVIMPGLGWLIATVLGLPPQ
                     LAAGVILVGCAPSGTASNVVTYLARGDVALSVSVATVSTLVAPLVTPPLTLLLADRFLD
                     VDAGAMLTDILKTVLVPVLGGALVRMAAGRWIGKVLRALPALSALTIAVLVLIVVSGSA
                     SRIKDAAALVLLAVVLHNGLGLALGYAAGRLARLGRPSSRAMAFEVGMQNSGLAASLAT
                     AHFSPAAALPAAIFSVWHNVSGAAVAAWMSRRPHP"
ORIGIN
        1 gaagccgctg gcgccggcgc gcagcgcgtc gtacacgtag ctgtcgttct cgaacgtggt
       61 gacgaccagg acgcgcggcg gggagtccat gccgccgatc agctgctcgg tggcccggat
      121 cccgtcgacc tcgggcatcc gtacgtccat caggacgacg tcggggcgca gttcgcggac
      181 gacggagacc gcttccgcgc cggtcgtggc ctcgccgacg acctccaggt cctcctcggc
      241 gcccaggatt gcccgcaggg cggtccgcac catgcgctcg tcgtcggcga gcacgatccg
      301 caggggcgcc gtcaccggcc cgcacccccg cccagcggca ggcggacggc gagccgccac
      361 accccgtccc gctcgccgga gctgctgctg ccgcgcagca gccgggcccg ttcggctatg
      421 cccgtcagac cgcggccgcc gccggtgcgg cggtgcgcgc ggccgggggc cttgtcgggg
      481 ttgaccggat tctccaaggc gacctccacg gcgtcgtcgt gcacggtgat ccgcagcagc
      541 acgggcaccc ggcccgcgtg ccgcaaggca ttggtgaggc cctcctgcac gatccggtac
      601 gcctcgcggg agacgaccgg cggcacgccg tccagggccg gaggtgaggc gagggtgacg
      661 gtgccgcccg cggcgcgggt gcgctccagc agcccgtcca ggtcggcgag ggtcggcgcg
      721 ggcgcggcgg acgcggcgcc gtcctcgcgc agcagcccca gcacggtgtc cagttcgccg
      781 accgcctcgc gcgtcgtctc ctcgatcgcg gccagcgcct gccgcgcgaa ctccgggtcg
      841 gcgtcgagga ccttgcgcgc cgcgctcgcc tgcagggtga ccgcgctcag ggcgtgcccc
      901 acggagtcgt gcagctcgcg cgccagccgg ttgcgcaggg ccagccgcat ggcgtgctgc
      961 tcggcctcgg ccagccggtc ggcggccgtc gggccgagga ggaggggtgc gcagcgggcg
     1021 aggagcgtgc ccgcgcccca ggacgccccg acgagcagga ccagcaccgc caggccggcg
     1081 ggcaccgcca gcagcgtcca cgggaaggcc aggtccccca gcccgtacgg ggcgtcctcg
     1141 ccccccggaa cacccgtgac cggcagggcg atcagcgtca gcgcgaacgg gggcgtggcc
     1201 agcgacatgc cgctcacgat gccgccggcc agcaggtgca gggtgtacca cagggcggtc
     1261 cgcccgcgcg cggcccgcga atcgcccggg cccgtcgcga gcgcctcctc cggcaccccg
     1321 cacagggcgc gtgccgccac cgactccagc gtccgcacca agggcaccag cgcggtcacc
     1381 gccgccggca ccagcgccag cgcgaagacg ccgaactgcc ggccgacggt ggaggggaac
     1441 aggatgttcg tgtcccggac gaagaccgcc gtcagcgtgc tcgccagcag gtagtacggc
     1501 atcagcagcg caccgccgag cagcaggtgc acccaccgca gccgggcgcg gcggcccacc
     1561 agggcgacgg cgccgcgccg gaggagagcc gtcacagggc ggcggcccgg gcggagcgct
     1621 ggacgaagaa gcgcgtgccg gcggcgagga ccagcagacc gatgatcact tgcacagcgt
     1681 aggcgacggc catcgtcgcc gtcggccccc ggccttcgtc ggccagggac gtcagcatcg
     1741 cgagcagttg ccagccgccc cacgcggccg aagcggccga gccgacgccc gcgaggagca
     1801 gcggcacccg cagcggcagc cggccgccgt gtccggaccg tgcccggccg gacagcagca
     1861 ggacggcacc cgcgatcgtc gcggcggcgt acagcacgta gcccgactcc ccggccgcga
     1921 aggtgccgct gtacatccgg gccatctccg gcgagaggcc cgccgacacc ccgcacgtcc
     1981 acatcaggtg gaccgcgccg ggcagcacgg ccagcactcc cgccgcggcc agtgcggccc
     2041 gctgcccgcg gccgggcgga cggcccgcca ggtccccgac gcggccccgc cacaggtggc
     2101 cccagcgctc gcgggcgtac agcacgaaca gggtccccag ggccagcccc tggacgatga
     2161 accccgtgta gaccacgccg aagacccact cgtcgaggaa cggctcgtcg gcctcgctcc
     2221 cggaaccgga cgccgaaccg cccagggcac ggacgatcaa ctgtgcggga aaggcgtaca
     2281 tgatgggcgc cagcagcccc accgcccccc acatcggcag cacgagcagc caggcgggga
     2341 cgcgcttgcc ccagggccgg gtcagcagca gggcgaggac cacgaccgcg gcgtccatca
     2401 ggatggtcac ggcgttcgcc acgaccatgc tcgtgtcggc atcccgcagg ccgcttccct
     2461 ccgggatgcc gatgtgactc cccgccaccc aggccgcctt gaggccgagg tagggcaggc
     2521 aggcggcgat ggtcaccgcg cggagcgcgc ggcgtttggt ggcgtggtcg tccgggacgg
     2581 tcgtcgtcgt actggtcatg tgaccagcgt tccgtccggc gggcgccggc gcgtcgccct
     2641 gagcggcgga ccgcctccgc cgcgaggcgg agggacggct agttctcagg caggggacgg
     2701 gaggcccgca cgggcggacc ggtggatgga ccactggacg gaccggcgga cggactgatg
     2761 aacaggctga tggctggact ggtggacgga ccgatggctg gactgatggc cggactgatg
     2821 ggcgtaccga tggctggacc gatggacgga ttgatcgccg gacgcgcata gtgggtgggc
     2881 atggagagcc tcgcccccgc acacttcgcg cccgagaccg tctacctcaa caccgccgcc
     2941 accgggctcg tgccctcgcc cgccgtcgcc gccatgaccg aggccgccac cgcgttctcg
     3001 ctgggccgcc ccggcgcgga ggaccacttc ccggtcgtgg aggccgtccg cgccacgttc
     3061 gcccggctcg tcggcgtgcc cgcccggcag gtcgccaccg ggggttcggc cgccgtcttc
     3121 tgcgggctcg tcgcccagtc gctgcccgag gagtcggaag tcctctgcgc ggagggggac
     3181 ttcagttccc tcgtcaaccc cttccacgtg cggcccgggc tccgcgtccg caccgttccc
     3241 ctggagaaga tcgcggacgc ggtggggccc gggaccgcgc tcgtcgccgt cagcgccgtc
     3301 cagtcggccg acggccgcct cgccggcctc ggggcgatcg gggaggccgc ccgcgcccac
     3361 ggcgcccgga cgctcgtcga cgtcagccag gccgcgggct ggctgcccgt cgacgcgggc
     3421 gcccacgact tcaccgtctg tgtcggctac aagtggctga tgggcccccg cggcacggcc
     3481 tacctcaccg tccccgagga cctcggcggc ctcacccccg tcttcgccgg gtgggtggcg
     3541 ggggaggacc cctgggacag cacgtacggg caggtgtcgc tcgcccgctc ggcccgccgc
     3601 tacgacgaga accccgccct cctgccctac accggcgccc tggccggcct ccggctcgtg
     3661 gaggagctcg ggacggagcg catcgccgca cacgaccggg ccctcgccga ccgcttccgc
     3721 acggggctcg ccgcgctcgg gcaccggtcc gccccggcgg aggactccgc gatcgtctcg
     3781 gtccccggcc tcggccgcgc ggcggagcgc ctcgcggcgg cgggggtacg cgtcagcgcc
     3841 cgcgccgggg gcctgcgggc gtccttccac ctctacaaca gcacggatga cgtggaccgg
     3901 tgcttggagg cgctggcctg agaggtcgcg gggcggggcg gggcgtgggc cggtgaagcc
     3961 cgggggcctt ccctccgccg caacggcgat ccgccacgac gccggtaccc ggcggtgccc
     4021 gcgacctgtc ggtcgggacg gcaccgccgg gcacgtccgg tgggggtgcg cgcggccggt
     4081 tacttcaccg gcgtgaagtc ccgcgccccc acgaattcag ggcggcgtct cggggccgcg
     4141 aacggctcga ccgccgtgtt ctccacgctg ttgaagacga tgaagacgtt gctgcgcggg
     4201 aacggcgtga tgttgtcgcc cgacccgtgc atgcagttgc agtcgaacca cgtcgccgaa
     4261 ccggccgcgc ccgtgaagag ccggatgccg tgccgtcccg ccagcgcggc gagcgcgctg
     4321 tccgacggcg tgccggcctc ctgcatccgc agggaccgct tgtagttgtc ccgcggcgtc
     4381 acgcccgcgc agcccaggaa cgtccggtgc gaacccggca tgatcatcag gctgccgttg
     4441 gtgtcgaggt tctccgtcag cgcgatcgac accgacaccg cacgcatccg cggcagcccg
     4501 tcctccgcgt gccaggtctc gaagtccgag tgccagtaga acccggaggc cccgaagccc
     4561 ggcttgacgt tgacccgcga ctggtggacg tagacgtccg agccgaggat ctgccgcgcc
     4621 cggcccacca cgcgcggatc gcgcacgagc cgcgcgaaca cctcgctgat ccggtgcact
     4681 tcgaacaccg accgcacgtc cttcgagtgc ggctcgacga cagctctctc gtcggcccgc
     4741 accgccggat cggcgatcag ccggtcgagc tcggcgcgca ggacctgcac ctcgtccggc
     4801 gtgatcatct cgtcggcggt gaggaacccg tcgcgttcgt agcccgcgag accggccgcg
     4861 tcgatcggcc cgggcgtgcc ggtgccggtg ccggcgccgg aggcggaccc ggacccgggc
     4921 cacaccacgg ggtcacggcg cgggacggcc acctcgtgcg tgccgcgcgt ggggtagagg
     4981 tcggtgaccg cggccatggc tcagccctcc tccgtcagca gcgggtagac gccgttctcg
     5041 tcgtggtcct cccgtcccgt gaccggcggg ttgaagacgc acacgcagcg gaagtcggtc
     5101 ttggggcgca gggtgtgccg ctcgtggccg tcgagcaggt acatcgtgcc gggcgagatc
     5161 cagtgcttct cgccggtctc gtcgtcggtg agctcggcct cgccctccac gcacagcacg
     5221 gcctcgatgt ggttggcgta ccacatggag gtctccgtgc cggcgtagag gacggtctcg
     5281 tgcagggaga agccgacccg ctccttggcg agcacgatcc gcttgctctc ccacgtaccg
     5341 gacttggcct tgacgtggcg gtcggtgttc tcgatgtcct ggagggaacg gacgatcacg
     5401 ggttgttcct ttctttcggt actaccggtg ccggtgccgg tggcggcaac ggtggtggtc
     5461 aggccgtctc gcggaccgca cgggcgacga tccgcaggcc ctcgtccagc tcgtcgggcg
     5521 agatcgtcag ggcgggcagc agcttgagga cctcgccctc ggggcccgac gtctccacga
     5581 gcaggccgag ctcgaacgcc cgccgggcga ccgcggaggc ccggggcttg tcggcgaact
     5641 ccagccccca cacgaggccg cggccccggt agtgcgcgcc cagctcgggg tgttcggcgc
     5701 agatggcgcg cagcgcctcc tcggcctgct cgccgcgggc gagggtctgc ttctccatct
     5761 gcccgtcggt ccagtacgcg tcgagcgccg cggcggcggt gacgaaggag gggttgttgc
     5821 cgcggaacgt gccgttgtgc tcgccgggct cccagacgtc gagctccggg cggaagaggg
     5881 tgagcgacat gggcaggccg tagccgccga tggacttcga gagggtcacg atgtccgggg
     5941 tgatgcccgc ctcctcgaac gagaagaagg cgccggtgcg gccgcagccc atctggatgt
     6001 cgtccacgat cagcagcatg tcgtggcgct cgcacagcgc ggcgagcccg cgcagccact
     6061 cggggcgggc gacgttgatg ccgccctcgc cctgcaccgt ctccacgatg acggcggcgg
     6121 gggtgttcag gcccgagccc tggtcggcca gcagccgctc gaaccacagg aagtccggca
     6181 cctggtcgtc caggtagtgg tcgaacggca tcggcgtgcc gtgcaccagg gggatgcccg
     6241 cgccggcccg cttgaaggcg ttgccggtga cggcgagcga gcccagcgac atgccgtgga
     6301 aggcgttggt gaaggacacg atcgcctcgc ggcccttgac cttgcgggcc agcttcagcg
     6361 cggcctccac ggcgttggtg cccgtcgggc cggggaacat caccttgtac ggcaggtcgc
     6421 gcggccgcag cacgacgtcc tggaaggcct ccaggaacgc gcgcttggcg gtggtgccca
     6481 tgtccaggcc gtgggtgacg ccgtcgcgtt cgatgtaatc gatcagggcg cgtttgagga
     6541 ccgggttgtt gtgcccgtag ttgagagccc cggcgccggc gaagaagtcc aggtacgtgt
     6601 ggccgtcctc gtcgtacatc cggctgccct gggcccggtc gaagaccgcg ggccagctgc
     6661 ggcagtagct gcgcacctcc gactccaggg tctcgaagac gctgagggcg ggcggggtga
     6721 tggtcatggg ggtgctcctg tcggtgcgtg agggttctca gggcgtgagg gttctcgggg
     6781 cgtgagcggt ctcagggcgt gagggttctc ggtgcgtgcg ggggcctcag tgcgtgcggg
     6841 gcctcagtgc gtgagggggc cgatgcggtg caggacctcg gggtcgtgcc ccgtctcggg
     6901 gaacagcccg ccgtggaaga ggacttcgcg ctccacgggc gcgccgtgcc gctcggcgaa
     6961 ggaggcgaac aggcggttgg aggcggtgtt gccgggcgtg acggtggtct cgatcccgtc
     7021 gatgccggtc tcgctctcgc gggcgacgcg tgcgacgagg ccctccagca gcgcggcggc
     7081 caggccgcgg ccgcggtgcg ggcggtccac ggcgacctgc cacaccacga gggtgcgggg
     7141 gcgggccggc cggacgtagc cggtgatgaa gccggcgggc tccccgtcgc cggtgcggcg
     7201 ggccacggcc gaggtggccg cgaagtcgcg gcaccacagc aggtagctgt acgaggagtt
     7261 gaggtccagg gttcccgagt cgcgggcgat gcgccagatc gcggctccgt cctcgacgcg
     7321 cgggacgtcg agggtgaggt cctccggcaa ttccgggaat tcccggaggg aggcgggacg
     7381 ggcacgtgca aggtctgctt gtgctgcggt catgcggagg gaatttaccg atgcggatcg
     7441 gaaatcgcat cgggggtccg ggttacccgg gggtgggtgg ttgtgttatc gcgcggaggc
     7501 gcgggggcgc gcaaagcggg tgtgggatgg gatggtttgc ccgttaatat ccgggcaaat
     7561 cgcagactgt gtggagtacg tcacaagtga tcgctggggt cgcgaattcg tttgcggaag
     7621 cgtaaggaaa atgctgcgtt tagggtgcgg aaaagcgggc atgagaatgc gcggagagtt
     7681 gtctccggaa ttatcggaga ggagttccgg ggaattgccc ggggcggtgc ccggccgcgc
     7741 cggccgaggg tccgccgggc atcggcggcg acccggccga gccccggcga cggccccttg
     7801 gcgggcggga ggagcgggct cgtacagtgc ggtccatgag cgacagtggg gtgctgcaca
     7861 tcaaggggcg ggtgctcgtc ggcgcggacc cggacggcgg ggtgcgcgac gagctgtggg
     7921 ccgtcgacgg ccggatcacc ttcgaacggc cggtgggggc gcgggacgtc accaccgtcg
     7981 agggctgggc actgcccggc ctcgtggacg cgcactgcca cgtgggcctc gacgcccacg
     8041 ggccggtcga cgaatccacc gccgagaagc aggcgctcac cgaccgggac gcgggagcgc
     8101 tgctgctgcg cgacgccggc tccccctcgg acacgcgctg gatcgacgac cgcgaggacc
     8161 tgccgcgcat catccgcgcc gggcgccaca tcgcgcgcac ccgccggtac atccgcaact
     8221 acgcgcacga gatcgagccc gacgacctcg tcgcgtacgt ggcgcgggag gcgcggcgcg
     8281 gggacgggtg ggtcaagctc gtcggcgact ggatcgaccg cgagacgggc gacctgtcct
     8341 cctgctggcc gcgcggggag gtcgcggccg cgatcgccga ggcgcaccgc ctcggggcgc
     8401 gcgtgaccgc gcactgcttc gcggaggagt cgctggcgcc gctcgtggaa gcgggcatcg
     8461 actgcatcga gcacgccacc gggctcaccg aggagaccat cccgctcttc gccgagcgcg
     8521 gggtcgccat cgtgccgacg ctcgtcaaca tcgcgacgtt cccgcggctc gcggacggcg
     8581 gcgagggcaa gttcccgcgc tgggcggacc acatgcggcg cctccacgcc cggcgctacg
     8641 agacggtgcg ggcggcgtac gacgcgggca tcccggtctt cgtcggcacg gacgcgggcg
     8701 gctccctcgc ccacggcctc gtgggccagg aggtggccga actcgtcaaa gccggcctgc
     8761 ccccgctcgc ggccctctcg gccaccacgt ggtcggcccg atcctggctc ggccgcccgg
     8821 gcctggagga gggcgccccg gcggacatcg tcgtctacga gacggacccg aggacggacg
     8881 tgagagtcct gggctcaccg aagcgggtga tcctgcgggg gagggtgatc cgctgaccgc
     8941 ggagcaccgg gcgacctgcg gtcggcttgg cccggggggc cggggggtcg gcgtgtgccc
     9001 gccgggctcg gtaacttccc tccgccgcgg cggtgatccg ccacgacggt gctgcccggc
     9061 ggtgcctggc ctccctgggg tcggtttggc accgccgggg gcgttcggtg ggggttgcgc
     9121 gccgtggcgg cggggagagg ggcgtggccc ggttggtgtg gcgggctgct tcggcgtggc
     9181 tccgggccgg gcaactttcc tccgccgcgg cggtgatcag ccacggcggt ggtgtccggc
     9241 ggtgcctgac gacctgcggt cgggtcgggg agcagggcgt gcccgccggg ctcggtaact
     9301 cccccccccc gcggcggtga tccgccacga cggtggtgcc tggcccgcct ggggtcggga
     9361 cggcgccgcc ggggggcgct gcggcggtgc aggagttgct gcccggctgg tggcgtgggc
     9421 ggcgtaggtg ttaggggtgg gggcgtcttg acatccaggc tgctaccgcc gcgcccgaca
     9481 cgttgtgcca taccgagaag atcgccgccg gcagcgccgc cgccgggctg aagtgggccg
     9541 tggccagtga cgccgccagg cccgagttct gcatgccgac ctcgaaggcc atggcccggc
     9601 tcgacgggcg gccgagccgg gcgagccggc ccgccgcgta gccgagggcg aggccgaggc
     9661 cgttgtggag gacgacggcg aggaggacga gcgcggccgc gtccttgatg cgggacgcgc
     9721 tgcccgagac cacgatgagg acgaggacgg cgatcgtcag cgccgagagc gccggcaggg
     9781 cgcgcagcac cttgccgatc caccgccccg cggccatccg cacgagcgcc ccgccgagca
     9841 ccggcacgag cacggtcttg aggatgtcgg tgagcatcgc gcccgcgtcc acgtccagga
     9901 accggtccgc cagcagcagc gtcaacggcg gggtgacgag cggcgcgacg agcgtggaga
     9961 cggtggcgac ggacacggac agggcgacgt ccccgcgcgc gaggtacgtg acgacgttgg
    10021 acgccgtccc gctcggcgcg cagccgacga ggatcacccc ggcggccagc tgcggcggca
    10081 gcccgagcac cgtggcgatc agccagccca gccccggcat gatcacgtag tgggcgacga
    10141 ggccgagccc gaccgcccag ggccgtttgg cgacggcgcg gaagtcgtcg ccggtgagcg
    10201 tgaggcccat cgtgaacatc acgacgccca gcagccaggg cacggcgggc gcccagccgg
    10261 tgaagtggga gggtgcggcg agccccaccc ctccggcggc gaggacgagc agggggaaca
    10321 cggtgacagc cagccgcgcc gtccggtcgg cggctgtcgg ggctatccgc ggggtcgaca
    10381 tacggcggca ccttagcga
//
